CN1380101A - Biological immunomodulator for preventing and curing mad cattle disease and its production method - Google Patents

Abstract

The present invention relates to a biological immune regulator for preventing and curing and cattle disease. It adopts the Micro-Alternating-field Biotechnology, called MAB for short, and uses yeast to make preparation, and is characterized by that it utilizes the above-mentioned technology to activate some corresponding cellular immune cryptomorphic functional gene in yeast, and makes the recessive gene be activated under the condition of that its structure gene portion is not changed in DNA sequence to implement high-efficiency expression of immunological function so as to obtain the injection and oral preparation of said biological immune regulator for preventing and curing mad cattle disease.

Description

The ox that can prevent and cure mad cattle disease is with biological immunomodulator and production method

The present invention relates to biologic product, particularly adopt micro-alternating bio-electric field (Micro-Alternating-field Biotechnology is called for short MAB) modulation radio ripple to produce, be used for the ox biologic product of prevention and treatment mad cattle disease.

At present, in Europe and some other place, the world and crazy disease appearred, these have been suffered from the ox of mad cattle disease, carried out mostly that killing disappears ruins, to prevent stretching of mad cattle disease, but can prevent or cure mad cattle disease without any medicine or immunomodulator at present, the people who got mad cattle disease does not then successively control, thereby people and the livestock of prevention and treatment mad cattle disease etc. extremely need with biological immunomodulator.

Purpose of the present invention is to provide a kind of and prevents and/or cure the brutish manufacture methods with biological immunomodulator of livestock such as the people of mad cattle disease and Niu, and this kind of producing with the method biological immunomodulator is provided. The object of the present invention is achieved like this, adopt a kind of like this production method of producing biological immunomodulator in micro-alternating bio-electric field (MAB), this biological immunomodulator is applicable to various livestocks, ox particularly, be used for prevention and device and/or cure mad cattle disease, it is characterized in that, adopt barms, and adopt following key step: adopt a kind of like this manufacture method that saccharomycete is made into the livestocks such as the ox usefulness immunomodulator with specific immunologic function, for giving anti-and curing various mad cattle diseases, it is characterized in that, the characteristic radio magnetic wave of employing micro-alternating bio-electric field activates the step of the latent function gene of every Yeasts, includes:

1. gene activation device (2) is set,

2. according to one-tenth assignment system culture medium first (3) P of subordinate list one, and sterilization treatment,

3. select the saccharomyces cerevisiae of IFFI1021 yeast specie, the IFFI1021 wine brewing is thin according to living

Born of the same parents/culture medium 〉=1 * 10⁸Individual ratio is injected the culture medium first, should cultivate first again

(3) put into the gene activation case (23) of gene activation device (2),

4. the temperature in the maintenance container was cultivated H1 hour in the T1 scope,

5. open electromagnetic wave transmitter (21), the output wave frequency is adjusted to gives fixed F model

In enclosing,

6. electromagnetic wave transmitter output electromagnetic field intensity is adjusted in the V1 scope,

7. the gene activation case (23) of the blake bottle of yeast IFFI1021 nutrient solution will be housed, according to

Pattern shown in the gene activation device (2) is installed to the receiver amplifier output, will

Receive frequency is adjusted in the identical F scope with the tranmitting frequency of transmitter (24). With

The time distance of regulating between transmitter (24) and the receiving instrument (25) be L1,

8. under these conditions, and the temperature conditions of maintenance T1, activate H2 hour,

9. IFFI1021 yeast cells after above condition activates adopts vacuum refrigeration in dry

Method for producing ampoules or a powder saved. Cattle and other livestock, and an immunomodulatory agent used for the prevention and treatment of a variety of mad cow disease I, Wherein the yeast is the use of the method for producing the above-described injectable preparation, and Oral gavage agent. Biological agents present invention has the following features and benefits: 1 analog gene-specific immunity "Life Radio" artificial wave method, with Completely different from the traditional life science research; (2) The active material has a rapid adjustment of life the immune function of the body; 3 rapid adjustment by the immune function, to livestock such as that with BSE Healthy people can quickly restore the body's effectiveness; 4 biological agents present invention is not medicine, not medicine, but not antibiotics, Interferons, hormones, etc., but a safe non-toxic side effects of biological agents; 5 is highly specific for different diseases caused by decreased immunity, damage Or missing must use a different biological immunomodulator. The method of the present invention, the theoretical basis and products as follows: Current biological techniques have been developed to gene stage, for a gene, while recognizing Its sequence but enzyme gene expression mechanism, the mechanism of enzymatic substrate, and enzymatic Of activity of the substrate is still difficult today biotechnology research, greatly limiting the biological Technology development. As mentioned above, why biotechnology research in this unfavorable situation? The present invention recognize The key is a research method for the problem. Conventional biotechnology research is used in biochemical Science, biological molecules and other methods, these methods are the conventional chemical methods evolve over The. Chemical methods is essentially a compound of nonliving matter, decomposition, redox Other changes. Biology is a living material, these living matter every minute of every hour Is constantly changing, the older generation dies generation rebirth alternately unchanged. Only in the composition The smallest unit of the body - the metabolism of cells, the conversion of substances and orderly conduct, Never stops. Today's biotechnology research to flesh this tangible material as life All actually very incomplete. The study suggests that the present invention, a living Organisms should be two basic parts, part tangible flesh Body, namely the physical substance; another part is the conventional theory that the invisible invisible Substance - "farm." Referred to herein, "field" is found in every living organism on "Life Radio." In a living body, this life constantly emit radio waves, When this invisible "Life Radio" after a loss, life will stop, the flesh will Become dead matter - corpses (the flesh). All the studies in the world today are only studied dead Substance - the flesh, but no one of "life waves." Sense The flesh is a dead matter, "Life Radio" is the living substance, flesh only "life Radio "host, namely emission source only when the flesh and the" Life Radio "organically Results Together can be called living matter - creatures. In summary, in the life sciences The research, "Life Radio" research study is more important than the flesh! Of course, "Health Life wave "research must be combined with the flesh in order to be a complete biological research. The present invention has been able to successfully obtained for a variety of disease prevention and control of biological immunomodulators Products, the key is the use of biotechnology research with different conventional methods. The present invention Involved in the research method is a combination of the organism's life essence, both of the living body Physical characteristics, but also to study life forms living material "life waves." The invention of "life Life Radio "to boarding a necessary condition in the flesh, but of" Life Radio "on Regulation of the composition of the host material. Through this research successfully won the world's first Biological immunomodulating agents. Experimental results show that immunomodulatory agent is safe, non-toxic side Effect, the effect is significant. So, for the prevention and treatment of human and bovine BSE and other raw Thereof. Cause of mad cow disease from pre to consider the following factors: Recent 200 years is highly developed chemical technology era, especially in the 20th century 70 Years of chemistry, biochemistry, biotechnology, etc. With the rapid development. Thus creating Several hundreds of millions of dollars made chemical products, from human use of tools, wearing clothes, food Food, decorative cosmetics and construction materials are chemical products; chemical make Into a traditional agricultural chemical agriculture, using either fertilizers, or prevention, pesticides, Weeding are chemical substances; in order to obtain high-yield crops, the use of chemical substances to stimulate the rapid growth of Is the use of chemicals. In medicine, especially in Western countries occupy 100% of the Market, is in China also occupied nearly 82% of the market. Large doses of antibiotics, hormones, Interferon and other kinds of chemical drugs everywhere. Either in the world today from The family still has the heart of every corner of the world, is full of chemicals Ling Lang everywhere. In recent years, many scientists believe that the chemical industry has brought a high level of human development, but also Meanwhile brought to mankind health hazards. This is because a large number of the ring caused by chemicals Environmental pollution, either human or animal consumption such as foods, or drinking water, are not With the extent of contamination by chemical; large number of chemical drugs, particularly antibiotics, Interferons, hormone drugs more dangerous; never even use of antibiotics, even from the To not take medicine and livestock animals can not escape from agricultural products, meat, eggs, milk and its Our products bring harm to the body substance. Ingest a lot of people and livestock, etc. Chemicals, resulting in a weird incurable diseases incurable. This is because the Various kinds of chemicals in humans and livestock, causing damage to the immune system, reduce or even Lacking. Also a large number of various chemical substances in the environment pollution induced the Various kinds of pathogenic microorganisms from a variety of reports in recent years shows that the world today Pathogenic microorganisms from both varieties, or from the pathogenic ability has been greatly improved. Thus creating a growing number of human diseases, more surprisingly, more and more difficult to treat. Ratio Such a wide variety of tumors, hepatitis, diabetes, uremia is very common, but when present Japan does not have a valid treatment approach. Throughout the world, various research institutions to Seen at a large number of scientists engaged in medical research, governments invested countless resources Gold support medical research, a variety of chemical drugs to market, however, is not only the traditional The disease has not been effectively controlled, but produced a new variety of strange diseases not Stop issued a challenge to the medical profession! Such as AIDS, mad cow disease, etc. were introduced, the medical profession beam Do nothing! Some data indicate that the use of antibiotics in the world today as many as 100 kinds of hormones are also a few Ten kinds, various chemicals are just countless. These antibiotics, hormones not only in the human Body to use, but also in livestock, poultry farming, aquaculture and other industries use More extensive and become an essential component of the feed, or the number of species are either Far more than the amount used in the human body. So eating meat, eggs, milk and products in the Remnants of a large number of antibiotics, hormones, and a variety of chemical drugs, indirectly, into human, animal body Within mutilation of the body of humans and livestock, with the use of this antibiotic was found not only This antibiotic is no longer so efficacious, but induced a new disease, a new pathogenic microorganisms. Caused by a variety of new incurable diseases, such as: BSE, H5N1 avian influenza virus. A variety of diseases is the enemy of livestock and human health, but also cause death of livestock and humans The death of the most important factor. Numerous studies indicate that, no matter what kind of diseases are associated with muscle The body's immune system related. When the body's immune system is strong variety of diseases will not happen. However, with various chemical substances, a variety of antibiotics, hormones, etc., causing the body Immune cell damage, cell metabolism, immune disorders, immune gene expression does not, Resulting in immune function decline. Immunity of the body is vulnerable to recession various disease Original invasion of bacteria, also subject to a variety of toxic substances harm, resulting in a variety of Kind of disease. Decreased body immunity according to the conventional detection method is difficult to send Now the. Since conventional methods detect body will find within the body of B, T, K, NK, etc. Normal numbers of immune cells. The study suggests that the present invention, the number of immune cells of normal and No body immune function can only be one of the indicators, but does not mean that the normal immune gene expression, But can not sign the nature of the immune enzyme, activity is normal. This means that the immune cells Not only the immune function of B, T, K, NK cells the number, but also to identify which These immune cells of the immune gene expression in normal immune whether the nature of the enzyme, the enzyme immune Catalytic activity is sufficient. The present invention is that the immune genes expressed only Immunoenzyme Number should be sufficient, and must have sufficient catalytic effect, that is, when the body B, T, K, NK cell count is normal, as expressed are not necessarily the number of immune enzyme sufficient quantities, when free When a sufficient number of Phytophthora enzyme, it is not necessarily a good catalytic activity. Immune genes and their His genes, divided into two parts according to their role (see Figure a), the left part of the Free Epidemic portion gene promoters, including the promoter. Promoter charged with starting Functional expression of the structural gene immunization the right amount of enzyme, the expression of function of time. Right Called functional structural gene portion, which is mainly structural genes, structural genes determine Expression of the gene structure of the enzyme, i.e. an enzyme properties, ie as long as the structural gene Jian motif Columns and curled chain gene structure unchanged, they express the enzyme structure and properties change does not occur Technology, but its expression and expression quantity time, when some of the table less enzyme, when expressed enzyme Amount more, when expressing the enzyme to peak so completely under the control of the left promoter. When the B, T, K, NK immune cells of the immune gene promoter sequence in part by a variety of Factors of harm, resulting in abnormal expression, it directly affects the functional structure of gene expression Immunoenzymatic number, expression time curve. When the immune gene function in part by structural gene To outside interference factor, causing its Xian group or curled shape changes, it will affect the function of junction Constitutive gene expression or the nature of the immune enzyme activity, the body's immune system has been Severely damaged, but routine testing is not easy to find. In addition, when the immune gene expression Immune enzyme quantity, nature is constant, the immune genes influence of harmful factors make express The reaction time is also very important to be consistent, which is said to be immune genes A capacity, when the immune response genes decreased ability to advance or delay the response time or when they Will exhibit a decline in immunity, resulting in body disease. Why is the body of B, T, K, NK cells of the immune genes have the problem then? The study found that many of the present invention with An anion or cation of the "free radicals", a variety of pathogenic microorganisms generated Toxic substances, as well as a variety of neurological disorders, from various sources and so may cause B, T, K, NK immune cell gene mutation or "rigid", rigidity of this gene, in the present Ming called "recessive gene", this recessive gene can not be timely, accurate expression, On the outside and a variety of pathogenic microorganisms pathogenic factors can not be timely to resist, causing the body to disease. After years of the present invention found that a variety of immune genes in the course of their life activities, Are of a very specific emission "life wave", different immune genes emitted "Life Radio" is different immune genes rely on these "life waves" transmit immunization information Against the body's immune response to harmful factors. So when the immune gene changes, The launch of the "life-wave" will change, which pipe is a small change is Resulting in "life wave" changes. Critical to the invention is the discovery of the gene of the "Health Life wave "caused due to harmful factors change, but can also be substances with a beneficial waves Under the control back to normal, caused by harmful factor "rigid" and "recessive gene", can With a beneficial factor activated. The present invention based on the above principles, the use of simulated body immune gene "Life Radio" artificial Waves, creating a "beneficial factor" and made from biological materials such beneficial factor system Agents, the use of such biological agents to control immune gene mutation, so as to regulate the immune Function. Through the regulation of immune function, enhance the body's ability to resist disease, So that the body of patients suffering from a variety of fast recovery. In the invention, a large number of studies suggest that, through artificial "Life Radio" was a Kind of gene activity can regulate immune substances, is a very difficult task. The present After 20 years of exploration Ming found the ubiquitous nature of microorganisms human use Only a "drop in the bucket", this is because people know much about microbial gene function. Today, all human beings to understand the composition and function of microbial genes only 26 species, and And it is also only 26 kinds of microorganisms kind of simple structure, for complex eukaryotic microorganisms person Class do not know. Especially humans frequently used yeast microorganism, containing a large not Be exploited genes that are not due to the long application has become a "rigid base Cause "of the present invention, said these" rigid gene "for" recessive genes. "Is more important Want is in these "recessive genes" in the present invention, there is need, can use Regulation of humans and livestock in the immune function of the body of the beneficial protein substances. The present invention will Is the use of artificial life-wave method to activate the yeast expression and regulation of the body immune to Functional protein substance "recessive genes" to achieve. The invention uses radio waves to activate the yeast life simulation "recessive genes", and encouraged The four kinds of active B, T, K, NK4 yeast species specific immune cells, made The regulation of the body immune function of biological agents. The present invention, in order to make these four species-specific Yeast, passed the stomach after eating, and can not be guaranteed in the stomach to kill a large number of acidic substances Dead, the present invention turn these four species-specific yeast resistant PH ≤ 2.5 the conditioned medium. Train After raising the yeast cells will successfully reach the small intestine through the stomach organs. Various enzymes in the small intestine Under specific yeast cells were lysed to release the intracellular packaging specific immunity Functional regulation of the enzyme (protein). These specific immune regulatory enzyme "specificity" of the shock Live, the regulation of immune cells in the body corresponding to the expression of immune genes. The invention relates to The four kinds of body immune specificity yeast, because they are human long-term consumption of livestock Or the microorganism used in the production, does not produce any toxic effects. These specific yeast Mother intracellular release of active enzyme, with instant activation, regulate immune genes within the body is Indeed, the role of high-level expression, and will vary within the body of metabolic activity in turn quickly lose Becomes the body of nutrients to be absorbed without causing the body of the residual. The present invention relates to the regulation of the body's immune function biologics brief description of the mechanism of As follows: regulation of biological agents in the body immune function is the core component of four kinds were stimulated with Live body B, T, K, NK "recessive immune gene" protein, which is divided into four kinds of proteins Do not concealed in four species-specific yeast. The four yeast artificial mode of the invention is to To be "life wave" active microorganisms, in the present invention, said in the four simulated "life Radio "activation" recessive genes "for the specificity of yeast yeast. These four species-specific Yeast cells were activated immune function containing enzyme, enzyme immune function of these Activation of specific immune B cells within the body, the immune T cells, immune cells and immune K NK cells to restore immune genes, activation, so that expression of these immune genes correctly and efficiently. This Yeast is some specificity through their specific simulation "Life Radio" condition cultivate Come. Activation of the present invention is used in the body-specific B-cell immunity yeast, the Use of artificial radio frequency and signal strength is determined by immune B cells of the immune genes Specificity "Life Radio" decisions; activate the body immune function-specific T cells of yeast, Used in artificial wave frequencies and signal strength is determined by immune T cells of the immune-based Due to specific "Life Radio" decisions; activate specific cellular immune function of the body K leaven Mother, the use of artificial analog radio frequency and signal strength, is free from the immune cells K Gene-specific epidemic "Life Radio" decisions; activated NK cell immune function special body Straight yeast, the use of artificial wave frequency and signal strength, is immune NK Cells of the immune gene-specific "Life Radio" decision. The four different functions Specific yeast, after recessive gene activation, simulated "Life Radio" condition training, so Produced within the cell having the activated immune genes control the four kinds of active enzyme (protein). Using these four specific yeast preparation, the specificity of the yeast into the body of the small intestine, in Under the action of many enzymes intestinal cell lysis, lysis of cells release immune gene activation Regulation of enzymes. These immune regulation of gene activation intestinal enzyme was immediately absorbed into the blood, through the With blood are transported to the four kinds of immune cells, thereby to achieve activation, four kinds of immune regulation Gene expression correctly improve immunity purposes. The present invention is bioregulators immune function, through the following two aspects of the steps. The first step is to use specific aspect analog "life wave" Activation Normal leaven Mother, so that they become a different yeast regulate B, T, K, NK cell immune function Specificity of yeast and yeast for resistance to these low PH conditioned medium; second aspect of the procedure In the specific analog "life wave" conditions, the expansion of the specific cultivation of yeast, Then these specific regulation of immune function in yeast made use of biological products. ...

It is bacterial classification gene structure key diagram that this specification includes following accompanying drawing: Fig. 1. Fig. 2 is latent function gene activation device key diagram. Fig. 3 is that the wireless mode of latent function gene is laid key diagram. Fig. 4 is the step key diagram of the method that is activated of latent function gene. Fig. 5 is strain domestication device key diagram. Fig. 6 is the step key diagram of the bacterial classification method of being tamed. Fig. 7 will pass through the square frame key diagram of the bacterial classification of domestication to the step of the biological immunomodulator of making the oral agents form. Fig. 8 is that special yeast enlarges the culture process schematic diagram after activating. Fig. 9 is specific yeast liquid hybrid technique schematic diagram. Figure 10 is the concentrated key diagram of specific yeast liquid. Figure 11 is cooling metering and finished product packing schematic diagram.

Below in conjunction with accompanying drawing, each feature of method of the present invention is described in further detail. Consult Fig. 1, Fig. 1 is bacterial classification gene structure key diagram. As previously mentioned, shown in the figure is gene structure display, and one section of the left side is promotor gene, and one section on the right is structural gene, and promotor gene has the functional structure gene expression immuno-enzymatic quantity that starts the right side and the function of catalytic activity. And structural gene has the immunoenzymatic effect of expression under the promotor gene in illustrated left side starts, as long as its structure is constant, the character of expressed enzyme can not change, but the time of its expression and expression is subject to the control of left side promotor gene. Consult Fig. 2, Fig. 2 is an embodiment key diagram of latent function gene activation device (2), shown in the figure, the device of present embodiment mainly includes frequency generator (21), amplifier (22), gene activation case (23), wherein, frequency generator (21) is connected with amplifier (22) phase telecommunication, normally the wired mode by transmission line connects, be provided with radial shield (231) in the gene activation case (23), amplifier (22) is linked on the radial shield (231) in the gene activation case (23) by output line, the bacterial classification that preparation is activated is placed in the gene activation case (23), open frequency generator (21) is to desired frequency F, amplifier (22) is that the life electric wave of F is amplified with the frequency of input, export by the power that gives provisioning request, be transferred on the radial shield (231), radial shield (231) activates the latent function gene of the bacterial classification in gene activation case (23) namely by frequency F radiation electric wave. Frequency generator (21) and amplifier (22) are electronic products commonly used, can directly buy or self manufacture in market, and radial shield (231) is the radio wave transmission antenna, can adopt the structures such as tabular, shaft-like, netted. Consult Fig. 3, Fig. 3 is the another embodiment of latent function gene excitation apparatus (2), the embodiment that adopts wireless laying mode, on Fig. 2 basis, changed, described device includes frequency generator (21), amplifier (22), gene activation case (23), particularly, can also include transmitter unit (24), receiving element (25), wherein, transmitter unit (24) is connected with frequency generator (21), the electric wave that frequency generator (21) occurs is exported away through transmitter unit (24), export to receiving element (25), the way of output can be wireless, also can be wired, receiving element (25) is connected with amplifier (22), receiving element (25) receives the electric wave signal that transmitter unit (24) is launched, when transmitter unit (24) is exported with the radio wave form, be connected without transmission line between receiving element (25) and the transmitter unit (25), as frequency generator (21) and the transmitter unit (24) of radiating portion, can separate with amplifier (22) and gene activation case (23) with the receiving element (25) as receiving unit. This set can bring convenience in some cases. As long as receiving element (23) can be received the signal that obtains emission part branch emission smoothly, be separated by far away also be fine, therefore, the life electric wave that sometimes can adopt the mode of wireless remote control to carry out bacterial classification excites. The most suitable employing wireless mode of the laying of the embodiment of this figure excites bacterial classification. Frequency generator among Fig. 2 and Fig. 3 (21) also usually is called electric wave transmitter (21), and transmitter unit (24) is installed in the electric wave transmitter (21) sometimes, and this specification also has employing. Consult Fig. 4, Fig. 4 adopts barms to carry out the method step key diagram of gene regulation. As everyone knows, common yeast is the zymophyte of the many kinds of substances such as class fermentation starch, carbohydrate, protide, multiplex bacterial classification in brewing alcoholic beverages, make bread, make various food, make medicine and multiple product thereof, although saccharomycetic various in style, Various Functions, but never the people utilizes the expressed specific proteins of yeast, activate respectively, regulate B, T, K, NK immunogene function, to carry out recessive gene in the method for not using patent of the present invention be not possess above-mentioned functions before activating to these yeast cells certainly. The below is used for regulation and control B cellular immune function gene yeasts " latent function gene " as example to activate, the present invention's method step in this regard is described: a large amount of gene technology studies have shown that, a complete B cellular immune function gene forms (seeing accompanying drawing one) by the two large divisions, a part is the promotor gene of B cellular immune function gene, and another part is the structural gene of B cellular immune function gene. The structural gene of B cellular immune function gene has determined its expressed immunoenzymatic character; The expressed immunoenzymatic quantity of structural gene, immunocompetence and immune response ability, i.e. immunoenzymatic the tiring of the promotor gene control B cellular immune function gene of B cellular immune function gene. Therefore, keep the character of the expressed enzyme of B cellular immune function gene constant, must manage to keep the structural gene dna sequence dna of B cellular immune function gene constant; The structural gene that reaches B cellular immune function gene efficiently expresses, and keeps the best immunocompetence of expressed immuno-enzymatic and immune response ability timely, must manage to make the promotor gene of B cellular immune function gene efficiently to start. The present invention activates yeast " latent function gene " by manual simulation's " life electric wave " method, obtains to have the specific yeast of expression regulation B cellular immune function gene protein. The present invention adopts manual simulation's " life electric wave " to activate the method for yeast " latent function gene ", it is characterized in that, comprise the steps: that 1. arrange bacterial classification active device (2), 2. prepare culture medium first (3) according to the composition of subordinate list one, and sterilization treatment:

Subordinate list one: activate regulation and control (B, T, K, NK) cell used yeast " recessiveness

Functional gene " the medium component table

Medium component	Quantity
		Sweet mellow wine	16g
K 2HPO 4	0.25g
		MgSO 4·7H 2O	0.2g
NaCL	0.22g
		CaSO 4·H 2O	0.5g
CaCO 3	6.0g
		Urea	0.2--0.5g
Serum	100--300ml
		Aquae destillata	700--900mL

3. select suitable yeast specie, selectable yeast sees attached list three, and subordinate list three is listed in this specification last part, optional wherein any one or more, the Candida arborea of genus class 14 or IFFI class saccharomyces cerevisiae etc. under for example selecting are according to active yeast cell/culture medium 〉=1 * 10⁸The ratio of Ge/1000ml, be injected among the training Raising Ji Jia (3); And cultivates the gene activation case (23) that the culture medium first (3) of injecting barms is put into bacterial classification active device (2), 4. the temperature in the maintainer gene maintainer gene activation case (23) is in the T1 scope, cultivated H1 hour, wherein, T1 can be between 37 ± 5 ℃, H1 can be between 24-56 hour, 5. open the frequency generator (21) of bacterial classification active device (2), to regulation and control B cell, output frequency is adjusted to the F1 scope. The F1 scope can be 6000M to 18000MHz, 6. when bacterial classification active device (2) adopts wireless transmission method shown in Figure 3, the electromagnetic field intensity of the output of transmitter unit (24) is adjusted to the E1 scope, E1 can be 230 ± 15mv/cm, 7. the receive frequency of receiving element (25) is transferred on the frequency consistent with transmitter unit (24) tranmitting frequency, regulate simultaneously the distance L 1 between transmitter unit (24) and the receiving element (25), L1 can be 100 ± 20cm, according to L1 and go out the output voltage V 1 of transmitter unit (24) according to the numerical computations of E1, when L1 is 100cm, output voltage V 1=(230 ± 15mv/cm) * 100cm=21.5V to 24.5V, when bacterial classification active device (2) adopts wired direct way of output shown in Figure 2, output voltage and field intensity are determined with reference to above-mentioned steps, make the field intensity in gene activation case (23) identical, 8. under these conditions, and the temperature conditions of maintenance T1, activate H2 hour, H2 can be 42-72 hour, 9. the yeast cells that activates through above condition adopts the method for vacuum freeze drying to make ampoule or make the pulvis preservation. The below illustrates respectively the example that activates regulating cell immunologic function gene, among following each embodiment, all adopt gene activation device (2) to Figure 3 shows that example: example one: the method that activate to be used for regulation and control B cellular immune function gene yeasts " latent function gene " is exemplified below: 1. according to the one-tenth assignment system culture medium first 1000-2000ml of subordinate list one, and sterilization treatment, 2. select the IFFI1021 yeast specie, ratio according to the IFFI1021 cell/culture medium of living, inject the interior culture medium first of gene activation case (23) of gene activation device (2) shown in the accompanying drawing 3,3. the temperature T 1 in the maintainer gene activation case (23) is between 37 ± 5 ℃, cultivating H1 is 24-56 hour, 4. open the electric wave transmitter (21) in the gene activation device (2) shown in the accompanying drawing 3, the output wave frequency is adjusted in the 6000M-18000MHz scope of F1,5. electric wave transmitter (21) is adjusted to through transmitter unit (24) output electromagnetic field intensity: in VF21.5-24.5V (take distance as 100com as the example) scope, 6. the gene activation case (23) of yeast IFFI1021 nutrient solution will be housed, be installed to the output of receiving unit amplifier (22) according to the pattern shown in the accompanying drawing 3, receive frequency and the tranmitting frequency of transmitter are adjusted in the identical F1 scope, the distance of regulating simultaneously between transmitter unit (24) and the receiving element (25) is 100cm, 7. under these conditions, and the temperature conditions of maintenance T1, activating H2 is 42-72 hour, 8. IFFI1021 yeast cells after above condition activates adopts the side of vacuum freeze drying to make ampoule or make the pulvis preservation. About activating the method step that is used for modulating T cell immunologic function gene yeast " latent function gene ": a large amount of gene technology studies have shown that, a complete T cellular immune function gene forms (seeing accompanying drawing one) by the two large divisions, a part is the promotor gene of T cellular immune function gene, and another part is the structural gene of T cellular immune function gene. The structural gene of T cellular immune function gene has determined its expressed immunoenzymatic character; The expressed immunoenzymatic quantity of structural gene, immunocompetence and the immuno-enzymatic of the promotor gene control T cellular immune function gene of T cellular immune function gene are answered ability, i.e. immunoenzymatic tiring. Therefore, keep the character of the expressed enzyme of T cellular immune function gene constant, must manage to keep the structural gene dna sequence dna of T cellular immune function gene constant; The structural gene that reaches T cellular immune function gene efficiently expresses, and keeps the best immunocompetence of expressed immuno-enzymatic and immune response ability timely, must manage to make the promotor gene of T cellular immune function gene efficiently to start. The present invention activates yeast " latent function gene " by manual simulation's " life electric wave " method, obtains to have the specific yeast of expression regulation T cellular immune function gene protein. Example two: the method that activate to be used for modulating T cell immunologic function gene yeast " latent function gene " is exemplified below: 1. according to the one-tenth assignment system culture medium first 1000-2000ml of subordinate list one, and sterilization treatment, 2. select the IFFI1212 yeast specie, according to IFFI1212 cell/culture medium 〉=1 * 10 of living⁸The ratio of Ge/1000ml, inject the culture medium first of gene activation case (23) shown in the accompanying drawing 3,3. maintainer gene activates temperature in the case (23) between T1=37 ± 5 ℃, cultivated H1=24-56 hour, 4. open the electric wave transmitter (21) shown in the accompanying drawing 3, to export wave frequency, to be adjusted to F2 be in the 7000M-19000MHz scope, 5. electric wave transmitter (21) output electromagnetic field intensity is adjusted to: in V1=21.5-24.5V (take distance as 100cm as the example) scope, 6. the gene activation case (23) of the blake bottle of yeast IFFI1212 nutrient solution will be housed, be installed to reception part amplifier (22) output according to the pattern shown in the accompanying drawing 3, receive frequency and tranmitting frequency are adjusted in the identical F2 scope, the distance of regulating simultaneously between transmitter unit (24) and the receiving element (25) is 100cm, 7. under these conditions, and the temperature conditions of maintenance T1, activate H2=42-72 hour, 8. IFFI1212 yeast cells after above condition activates adopts the method for vacuum freeze drying to make ampoule or make the pulvis preservation. About activating the method step that is used for regulation and control K cellular immune function gene yeasts " latent function gene ": a large amount of gene technology studies have shown that, a complete K cellular immune function gene forms (seeing accompanying drawing one) by the two large divisions, a part is the promotor gene of K cellular immune function gene, and another part is the structural gene of K cellular immune function gene. The structural gene of K cellular immune function gene has determined its expressed immunoenzymatic character; The expressed immunoenzymatic quantity of structural gene, immunocompetence and immune response ability, i.e. immunoenzymatic the tiring of the promotor gene control K cellular immune function gene of K cellular immune function gene. Therefore, keep the character of the expressed enzyme of K cellular immune function gene constant, must manage to keep the structural gene dna sequence dna of K cellular immune function gene constant; The structural gene that reaches K cellular immune function gene efficiently expresses, and keeps the best immunocompetence of expressed immuno-enzymatic and immune response ability timely, must manage to make the promotor gene of K cellular immune function gene efficiently to start. The present invention activates yeast " latent function gene " by manual simulation's " life electric wave " method, obtains to have the specific yeast of expression regulation K cellular immune function gene protein. Example three: the method that activate to be used for regulation and control K cellular immune function gene yeasts " latent function gene " is exemplified below: 1. according to the one-tenth assignment system culture medium first 1000-2000ml of subordinate list one, and sterilization treatment, 2. select the IFFI1301 yeast specie, according to IFFI1301 cell/culture medium 〉=1 * 10 of living⁸The ratio of Ge/1000ml, inject the culture medium first of gene activation case (23) shown in the accompanying drawing 3,3. maintainer gene activates temperature in the case (23) between T1=37 ± 5 ℃, cultivated H1=24-56 hour, 4. open the electric wave transmitter (21) shown in the accompanying drawing 3, to export wave frequency, to be adjusted to F3 be in the 8000M-17000MHz scope, 5. electric wave transmitter (21) output electromagnetic field intensity is adjusted to: in F1=21.5-24.5V (take distance as 100cm as the example) scope, 6. the gene activation case (23) of the blake bottle of yeast IFFI1301 nutrient solution will be housed, be installed to reception part amplifier (22) output according to the pattern shown in the accompanying drawing 3, receive frequency and tranmitting frequency are adjusted in the identical F3 scope, the distance of regulating simultaneously between transmitter unit (24) and the receiving element (25) is 100cm, 7. under these conditions, and the temperature conditions of maintenance T1, activate H2=42-72 hour, 8. IFFI1301 yeast cells after above condition activates adopts the method for vacuum freeze drying to make ampoule or make the pulvis preservation. About activating the method step that is used for regulation and control NK cellular immune function gene yeasts " latent function gene ": a large amount of gene technology studies have shown that, a complete NK cellular immune function gene forms (seeing accompanying drawing one) by the two large divisions, a part is the promotor gene of NK cellular immune function gene, and another part is the structural gene of NK cellular immune function gene. The structural gene of NK cellular immune function gene has determined its expressed immunoenzymatic character; The expressed immunoenzymatic quantity of structural gene, immunocompetence and immune response ability, i.e. immunoenzymatic the tiring of the promotor gene control NK cellular immune function gene of NK cellular immune function gene. Therefore, keep the character of the expressed enzyme of NK cellular immune function gene constant, must manage to keep the structural gene dna sequence dna of NK cellular immune function gene constant; The structural gene that reaches NK cellular immune function gene efficiently expresses, and keeps the best immunocompetence of expressed immuno-enzymatic and immune response ability timely, must manage to make the promotor gene of NK cellular immune function gene efficiently to start. The present invention activates yeast " latent function gene " by manual simulation's " life electric wave " method, obtains to have the specific yeast of expression regulation NK cellular immune function gene protein. Example four: the method that activate to be used for regulation and control NK cellular immune function gene yeasts " latent function gene " is exemplified below: 1. according to the one-tenth assignment system culture medium first 1000-2000ml of subordinate list one, and sterilization treatment, 2. select the IFFI1048 yeast specie, ratio according to IFFI1048 cell/culture medium 〉=1 * 108 Ge/1000ml that lives, inject the culture medium first of gene activation case (23) shown in the accompanying drawing 3,3. maintainer gene activates temperature in the case (23) between T1=37 ± 5 ℃, cultivated H1=24-56 hour, 4. open the electric wave transmitter (21) shown in the accompanying drawing 3, to export wave frequency, to be adjusted to F4 be in the 8000-16000MHz scope, 5. electric wave transmitter (21) output electromagnetic field intensity is adjusted to: in V1=21.5-24.5V (take distance as 100cm as the example) scope, 6. the gene activation case (23) of the blake bottle of yeast IFFI1048 nutrient solution will be housed, be installed to reception part amplifier (22) output according to the pattern shown in the accompanying drawing 3, receive frequency and tranmitting frequency are adjusted in the identical F4 scope, the distance of regulating simultaneously between transmitter unit (24) and the receiving element (25) is 100cm, 7. under these conditions, and the temperature conditions of maintenance T1, activate H2=42-72 hour, 8. IFFI1048 yeast cells after above condition activates adopts the method for vacuum freeze drying to make ampoule or make the pulvis preservation. But the hundreds of saccharomycete that provided 54 genus in this specification subordinate list three are choice for use all, shows listed microorganism but microbe species involved in the present invention is not limited to this. The activation of this moment the saccharomycete of the various cellular immune function genes of regulation and control of latent function gene be exactly the product of method of the present invention, namely biological immunomodulator can make the injection injection and use. The below provides the biological immunomodulator injection effectiveness test example of making as stated above. Experiment for example experiment gives an example one: a. gets 60 of wates rats, is divided into totally 3 groups of A, B, C, every group of 20 rats. The A group is experimental group, and the B group is for using the cyclophosphamide-a control group, and C is the blank group. B. adopt 180 ascites tumors, big white mouse is respectively organized in inoculation respectively. C. from postvaccinal second day, A organizes that (4 species specificity yeast cells all 〉=1 * 10 to this biologic product liquid⁸Ge/ml), dosage is 0.6ml/kg; B associative ring phosphamide, dosage are 20ug/kg; C organizes to physiological saline 0.6ml/kg. Once a day. D. dissect to detect afterwards the size of ascites tumor in seven days, such as following table:

Experiment gives an example two: a. gets 90 of wates rats, is divided into totally 3 groups of A, B, C, every group of 30 rats. The A group is experimental group, and the B group is for using the cyclophosphamide-a control group, and C is the blank group. B. adopt the U-14 solid tumor, big white mouse is respectively organized in inoculation respectively. C. from postvaccinal second day, A organizes that (4 species specificity yeast cells all 〉=1 * 10 to this biologic product liquid⁸Ge/ml), dosage is 0.6ml/kg; B associative ring phosphamide, dosage are 20ug/kg; C organizes to physiological saline 0.6ml/kg. Once a day. D. take and dissected afterwards the size that ascites tumor is washed in inspection in 14 days, such as following table:If adopt orally to gavage or add when feeding in the feed, the processing of the domestication that bacterial classification also must acidproof through specific yeast (anti-low PH). Illustrated that more than the present invention adopts the method for manual simulation's " life electric wave ", activate respectively yeast difference " latent function gene ", obtain 4 kinds of specific yeasts that are respectively applied to activate, regulate and control B cellular immunity gene, T cellular immunity gene, K cellular immunity gene and NK cellular immunity gene function. But this 4 species specificity yeast can't be directly used in the oral various immunogenes of regulation and control after the stomach effect that gavage, and this is because they can't adapt to the environment of human body. As everyone knows, be a very complex environment in the human body, and various envirment factor is all changing work all the time. When this 4 species specific yeast enters the approach of small intestine by oral cavity, stomach, be difficult to ensure its ferment born of the same parents' integrality, more therefore the activity of purpose in the Customers ' Legal Right yeast cells ensures that the yeast cells activity is very crucial. The present invention has adopted the envirment factor domestication of 4 species specificity yeast has been cultivated, and the domestication and culture method step is following to be described. The environmental suitability domestication of 4 kinds of functional microorganisms of the present invention is by realizing such as the device of Fig. 5 and method shown in Figure 6. Consult Fig. 5, an embodiment key diagram of the strain domestication device (6) that Fig. 5 adopts when being the specific yeast domestication of acid-resistance. Shown in the figure, described domesticating device comprises frequency generator (21), strain domestication tank (26), wherein, electric wave radial shield (261) is installed in the strain domestication tank (26), and the output of frequency generator (21) is connected on the electric wave radial shield (261) by transmission line. When domestication, the bacterial classification after putting into corresponding culture medium and be activated in strain domestication tank (26) is transferred to the frequency that will tame with frequency generator (21), can carry out the electric wave domestication. Consult Fig. 6, Fig. 6 processes the specific yeast bacterium that has produced certain cellular immune function gene through activating, the key diagram of the step of taming, bacterial classification after being activated will be tamed, could adapt to the livestock vivo environments such as oral cavity and stomach, the domestication step is (to adopt the 1000ml culture medium as example): 81. arrange domesticating device (6), 82. preparation domestication is with culture medium second (7), 83. get the saccharomycete that institute among the result of some aforementioned method steps activates rear preservation

Kind, to pour in the strain domestication tank (26), the quantity that adds is according to demand with set

Domesticating device scale (2) and deciding during as an example of the 1000ml culture medium example, adds such

Species specificity yeast juice 10ml (yeast juice living cells content 〉=1 * 10 of cell⁸Ge

/ ml), 84. pour the culture medium second (7) of respective amount into bacterial classification A tames in the tank (26), for example injects

1000ml, 85. open frequency generators (21), will export electric wave frequently be adjusted to regulation and control such cell exempt from

On the specific yeast selectivity frequency F of epidemic disease gene, namely, B is thin when regulation and control

During the specific yeast of born of the same parents' immunogene, get frequency F1, when modulating T cell is exempted from

During the specific yeast of epidemic disease gene, get frequency F2, when regulation and control K cellular immunity base

During the specific yeast of cause, get frequency F3, when regulation and control NK cellular immunity gene

During specific yeast, get frequency F4, F1 wherein, F2, F3, F4 are aforementioned

Each corresponding frequencies, electric wave is exported electric field regulates by 5-10mv/ml, 1000ml

The employed received-signal strength of culture medium second is the 5-10 volt, by strain domestication tank (26)

In electric wave radial shield (261) bacterial classification in strain domestication tank (26) is tamed

Process, 86. acclimation temperatures are T2, and the domestication time is H2 hour, and T2 can get 37 ± 5 ℃,

H2 can get 48 to 96 hours, was kept under 0-4 ℃ the condition strain separating for subsequent use after 87. domestications. Culture medium second (7) composition such as subordinate list two subordinate lists two of special use in the above-mentioned steps, culture medium second component list (take 1000ml as example)

Medium component	Quantity	Explanation
			Wild Jujube Juice	300ml	With the clear liquid that dry acid jujube/water=the 1g/5ml ratio is made
Malus baccata juice	500ml	With the clear liquid that dried Malus baccata/water=the 1g/5ml ratio is made
			(NH4) 2SO 4	0.25g
K 2HPO 4	0.2g
			MgSO 4·7H 2O	0.22g
NaCL	0.5g
			CaSO 4·2H 2O	0.3g
CaCO 3	3.0g
			Specific yeast nutrient solution after the activation	Each 20ml	Contain active yeast cell 〉=1 * 108Individual/ml

The bacterial classification of this moment is cultivated through enlarging, and can be made into oral agents, takes to human and livestock birds and beasts. The method step of the present invention's 4 species specificity yeast environmental suitabilities domestication is described as follows respectively, equipment therefor is take Fig. 5 as example: (one). and the specific yeast step 1. of domestication regulation and control B cellular immunity gene configures culture medium second according to the method for subordinate list two, and sterilization treatment, 2. get in the strain domestication tank (26) that culture medium second 1000ml is injected into accompanying drawing 5,3. get species specificity yeast juice 10ml (yeast juice living cells content 〉=1 of regulation and control B cell

* 108 Ge/ml) inject the strain domestication tank (26) shown in the accompanying drawing 5,4. open wave generator (21) as shown in Figure 5, and are adjusted to regulation and control B cell and exempt from

On the specific yeast selectivity frequency F1 of epidemic disease gene, the electric wave output electric field of 5. regulating as shown in Figure 5 is that (1000ml cultivates 5-10mv/ml

The employed received-signal strength of base is 5-10v), 6. keep above-mentioned wave frequency and electric-field intensity constant, at 37 ± 5 ℃ temperature conditions

Cultivate after 48-96 hour, separate under the condition that is kept at 0-4 ℃ for subsequent use. (2). the specific yeast step 1. of domestication modulating T cell immunogene configures cultivation second according to the method for subordinate list two, and sterilization treatment, 2. get in the strain domestication tank (26) that culture medium second 1000ml is injected into accompanying drawing 5 species specificity yeast juice 10ml (yeast juice living cells content 〉=1 of 3. getting modulating T cell

×10 ⁸Ge/ml) injects the strain domestication tank (26) shown in the accompanying drawing 5,4. opens wave generator (21) as shown in Figure 5, and is adjusted to modulating T cell and exempts from

On the specific yeast selectivity frequency F2 of epidemic disease gene, the electric wave output electric-field intensity of 5. regulating as shown in Figure 5 is 5-10mv/ml (1000ml

The employed received-signal strength of culture medium is 5-10v), 6. keep above-mentioned wave frequency and received-signal strength constant, at 37 ± 5 ℃ temperature conditions

Cultivate after 48-96 hour, separate under the condition that is kept at 0-4 ℃ for subsequent use. (3). the specific yeast step 1. of domestication regulation and control K cellular immunity gene configures culture medium second according to the method for subordinate list two, and sterilization treatment, 2. get in the strain domestication tank (26) that culture medium second 1000ml is injected into accompanying drawing 5,3. get species specificity yeast juice 10ml (yeast juice living cells content 〉=1 of regulation and control K cell

×10 ⁸Ge/ml) injects the strain domestication tank (26) shown in the accompanying drawing 5,4. opens wave generator (21) as shown in Figure 5, and is adjusted to regulation and control B cell and exempts from

On the specific yeast selectivity frequency F3 of epidemic disease gene, the electric wave output electric-field intensity of 5. regulating as shown in Figure 5 is 5-10mv/ml (1000ml

The employed received-signal strength of culture medium is 5-10v), 6. keep above-mentioned wave frequency and received-signal strength constant, at 37 ± 5 ℃ temperature conditions

Cultivate after 48-96 hour, separate under the condition that is kept at 0-4 ℃ for subsequent use. (4). the specific yeast step 1. of domestication regulation and control NK cellular immunity gene configures culture medium second according to the method for subordinate list two, and sterilization treatment. 2. get in the strain domestication tank (26) that culture medium second 1000ml is injected into accompanying drawing 5,3. get species specificity yeast juice 10ml (the yeast juice living cells content of regulation and control NK cell

≥1×10 ⁸Ge/ml) injects the strain domestication tank (26) shown in the accompanying drawing 5,4. opens wave generator (21) as shown in Figure 5, and is adjusted to regulation and control B cell and exempts from

On the specific yeast selectivity frequency F4 of epidemic disease gene, the electric wave output electric-field intensity of 5. regulating as shown in Figure 5 is 5-10mv/ml (1000ml

The employed received-signal strength of culture medium is 5-10v), 6. keep above-mentioned wave frequency and received-signal strength constant, at 37 ± 5 ℃ temperature conditions

Cultivate after 48-96 hour, separate under the condition that is kept at 0-4 ℃ for subsequent use. Consult Fig. 7, Fig. 7 is with through the bacterial classification of the domestication square frame key diagram to the step of making the oral biological immunomodulator that gavages the agent form. Specific yeast involved in the present invention is cultivated totally 4 kinds. This 4 species specificity yeast only is to have obtained seed (71) after obtaining through said method, make a large amount of biologic products of the present invention, and the specific yeast of capacity must be arranged, and therefore needs to enlarge and cultivates. The 4 species specificity yeast juices (72) that the present invention obtains through above specific yeast culture process respectively, this yeast juice is alone also can, but mix with then effect is better, therefore, as required, select several for example two kinds, three kinds, four kinds to mix, for example send into the hybrid technique step (73) of 4 primary yeast liquid. 4 species specificity yeast juice mixtures enter concentration step (74), then carry out canned (75), become finished product. Consult Fig. 8, Fig. 8 is that special yeast enlarges the cultivation schematic diagram after activating, the standby yeast of the spy who adopts enlarges culture process device (8) and includes frequency generator (21) and three culture tank (A, B, C), be provided with electric wave expelling plate (81) in each culture tank, frequency generator (21) is connected with each electric wave expelling plate (81) by output line, after frequency generator (21) is opened, the electric wave of its output through electric wave expelling plate (81) at corresponding culture tank (A, B, C) emission enlarges cultivation to special yeast in. The frequency generator here (21) can be identical with aforesaid frequency generator. The below describes respectively the cultivation of the specific yeast of the immunogene function of B, T, K, NK cell. About the specific yeast culture process journey of the specific yeast culture process adjusting involved in the present invention B cellular immune function of regulating B cellular immunity gene function as shown in Figure 8. Consult Figure of description 8 of the present invention, the specific yeast culture process step of adjusting B cellular immune function involved in the present invention is as follows: the 81.1 one-tenth assignment system culture mediums the third according to subordinate list four, and after sterilization treatment, annotate respectively

Enter in culture tank 8A, 8B to this accompanying drawing 8, the 8C tank, 81.2 will activate through manual simulation's life electric wave method, and through tolerating low PH (less than PH2.5)

The specific yeast of the adjusting B cellular immune function that obtains of cultivation, be input to

Among the seeding tank 8A shown in this accompanying drawing 8, as seed liquor, then according to necessarily

Ratio 8A tank seed liquor is injected into enlarge in the culture medium of 8B tank and cultivates,

The ratio of injecting is: 8A seed liquor/8B nutrient solution=5ml/1000ml, 81.3 regulate wave generators (21), making it export biological electric wave is F1, and simultaneously according to

0.5-1.0v/L requirement calculate, set received-signal strength (as cultivating in the hypothesis 8B tank

The quantity of liquid is 50L, and single intensity of wave should be: 0.5-1.0v/L * 50L=25v-50v), 81.4 keep in above-mentioned wave frequency and the constant situation of received-signal strength, under 37 ± 5 ℃ of conditions

Cultivated 56-72 hour, the 81.5 specific yeast living cells of regulating B cellular immunity gene in the B tank reach 20

During hundred million/ml, in the defeated 8C tank of 8B tank yeast juice, prepare to be transported to lower road

Hybrid technique. The present invention relates to regulate the specific yeast culture process engineering of T cellular immune function shown in this accompanying drawing 8 about the specific yeast culture process of regulating T cellular immunity gene function. Consult this accompanying drawing 8, the specific yeast culture process step of adjusting T cellular immune function involved in the present invention is as follows: the 82.1 one-tenth assignment system culture mediums the third according to subordinate list four, well are annotated respectively after sterilization place li

Enter in culture tank 8A, 8B to this accompanying drawing 8, the 8C tank, 82.2 will activate through manual simulation's life electric wave method, and through tolerating low PH (less than PH2.5)

The specific yeast of the adjusting T cellular immune function that obtains of cultivation, be input to

Among the seeding tank 8A shown in this accompanying drawing 8, as seed liquor, then according to necessarily

Ratio 8A tank seed liquor is injected into enlarge in the cultivation of 8B tank and cultivates, annotate

The ratio that enters is: 8A seed liquor/8B nutrient solution=5ml/1000ml, 82.3 regulate wave generators (21), making it export biological electric wave is F2, and with according to

0.5-1.0v/L requirement calculate, set received-signal strength (as cultivating in the hypothesis 8B tank

The quantity of liquid is 50L, and single intensity of wave should be: 0.5-1.0v/L * 50L=25v-50v), 82.4 keep in above-mentioned wave frequency and the constant situation of received-signal strength, under 37 ± 5 ℃ of conditions

Cultivated 56-72 hour, the 82.5 specific yeast living cells of regulating T cellular immunity gene in the B tank reach 20

During hundred million/ml, in 8B tank yeast juice input 8C tank, prepare to be transported to lower road

Hybrid technique. About the specific yeast culture process engineering of the specific yeast culture process adjusting involved in the present invention K cellular immune function of regulating K cellular immunity gene function shown in this accompanying drawing 8. Consult this accompanying drawing 8, the specific yeast culture process step of adjusting K cellular immune function involved in the present invention is as follows: the 83.1 one-tenth assignment system culture mediums the third according to subordinate list four, well are after sterilization treatment, respectively

Be injected in culture tank 8A, 8B, the 8C tank of this accompanying drawing 8,83.2 will activate through manual simulation's life electric wave method, and through tolerate low PH (less than

The specificity ferment of the adjusting K cellular immune function that cultivation PH2.5) obtains

Mother is input among the seeding tank 8A shown in this accompanying drawing 8, and is as seed liquor, right

Be injected in the culture medium of 8B tank according to certain ratio 8A tank seed liquor afterwards and expand

The large cultivation, the ratio of injection is: 8A seed liquor/B nutrient solution=5ml/1000ml, 83.3 regulate wave generator (21), and making it export biological electric wave is F3, and presses simultaneously

Requirement according to 0.5-1.0v/L is calculated, and sets received-signal strength (as training in the hypothesis 8B tank

The quantity of supporting liquid is 50L, and single intensity of wave should be: 0.5-1.0v/L * 50L-25v-

50v), 83.4 keep training under 37 ± 5 ℃ of conditions in above-mentioned wave frequency and the constant situation of intensity of wave

Supported 56-72 hour, the 83.5 specific yeast living cells of regulating K cellular immunity gene in the 8B tank reach 20

During hundred million/ml, in 8B tank yeast juice input 8C tank, prepare to be transported to down

The road hybrid technique. About the specific yeast culture process engineering of the specific yeast culture process adjusting involved in the present invention NK cellular immune function of regulating NK cellular immunity gene function shown in this accompanying drawing 8. Consult that this is attached 8, the specific yeast culture process step of adjusting NK cellular immune function involved in the present invention is as follows: the 84.1 composition preparation culture mediums the third according to subordinate list four, and after sterilization treatment, respectively

Be injected in culture tank 8A, 8B, the 8C tank of this accompanying drawing 8,84.2 will activate through manual simulation's life electric wave method, and through tolerate low PH (less than

The specificity ferment of the adjusting NK cellular immune function that cultivation PH2.5) obtains

Mother is input among the seeding tank 8A shown in this accompanying drawing 8, and is as seed liquor, right

According to certain ratio 8A tank seed liquor is injected in the culture medium of 8B tank afterwards

Enlarge and cultivate, the ratio of injection is: 8A seed liquor/B nutrient solution

=5ml/1000ml, 84.3 regulate wave generator (21), and making it export biological electric wave is F4, and presses simultaneously

Requirement according to 0.5-1.0v/L is calculated, and sets received-signal strength (as training in the hypothesis 8B tank

The quantity of supporting liquid is 50L, and single intensity of wave should be: 0.5-1.0v/L * 50L=25v-

50v), 84.4 keep in above-mentioned wave frequency and the constant situation of received-signal strength 37 ± 5 ℃ of conditions

Lower cultivation 56-72 hour, the 84.5 specific yeast living cells of regulating NK cellular immunity gene in the 8B tank reach

During 2,000,000,000/ml, in the defeated 8C tank of 8B tank yeast juice, prepare to be transported to

Lower road hybrid technique. Consult Fig. 9, Fig. 9 is specific yeast liquid hybrid technique schematic diagram, the mixing arrangement (9) that adopts includes 4 storage tanks (9A, 9B, 9C, 9D) and a blending tank (M), B, T, K, NK Cell regulate yeast juice have been stored up respectively in 4 storage tanks, usually these all are large tanks, have used literal as decorating and explanation on the tank surface. 4 related species specificity yeast juices of the present invention mix and can according to different targets, adopt different proportion to mix. In this example, be to mix according to the ratio of following table. Specific yeast liquid mixed proportion list (in the 4000L mixed liquor)

Specific yeast liquid kind	Quantity	Ratio	Requirement
				Regulation and control B cellular immunity gene function yeast juice	1000L		25％	In nutrient solution
Modulating T cell immunogene function yeast juice	1000L		25％						In nutrient solution
				Regulation and control K cellular immunity gene function yeast juice	1000L		25％	In nutrient solution
Regulation and control NK cellular immunity gene function yeast juice	1000L		25％						In nutrient solution

The mixing of specific yeast liquid of the present invention is to carry out according to the mode of this accompanying drawing 9. Consult this accompanying drawing 9, this technique realizes by following steps: 91. are input to respectively storage tank 9A, 9B, 9C, 9D with 4 species specificity yeast juices

In the tank, 92. with 4 species specificity yeast juices in storage tank 9A, 9B, 9C, the 9D tank according to

The ratio of equivalent is input among the blending tank M mixes, and 93. are injected into mixed yeast juice in the concentration technology shown in the accompanying drawing 10, prepares

Concentrated. Consult Figure 10, Figure 10 is specific yeast liquid concentration technique key diagram, and shown in the figure, device therefor is thickener 10A and 10B, has used literal as decoration and explanation on the thickener surface, and has used arrow explanation mixed liquor to flow to. Concentration technology is with the mixed liquid concentration of 4 species specificity yeast juices in the above-mentioned hybrid technique, and concentrated purpose is that the requirement when reaching the embedding finished product is set. Concentration technology is divided into two-stage, and being transported to thickener 10B after the first order concentrates with thickener 10A, to carry out the second level concentrated again, and finally reaching enrichment is about 64%. Concentrating of different in nature yeast juice involved in the present invention, realize according to following steps: 10.1 will through the mixed mixed liquor of above-mentioned hybrid technique, be transported to originally shown in Figure 10

Among the concentration technology first order thickener 10A, 10.2 are concentrated into the specific yeast mixed liquor in the tank of first order thickener 10A

80% (measurement basis calculation), then be transported among the thickener 10B of the second level concentrated,

Also can adopt the concentrated or side such as concentrated that heats of cold air Vacuum Concentration, normal temperature partial vacuum

Method, but all must be with the activity of retention performance yeast cells without which kind of concentrated mode

Benchmark, 10.3 are concentrated into 80% again in the tank of second level thickener 10B, about what concentrate

Condition when requiring still to concentrate with the first order is identical, is transported to immediately after concentrating

Dosing technology is prepared potting. Figure 11 is cooling metering and finished product packing schematic diagram, be called for short the dosing technology step, device therefor shown in the figure includes cooler (11A), dosing machine (11B), potting machine (11C), with finished product bottle (11D), used corresponding text decoration on each machine, with arrow flow sequence is described among the figure. Dosing technology involved in the present invention is cooling, metering and three processes of embedding. The purpose of cooling is that the temperature rise that causes in the concentration process will be got off, in order to avoid produce gas after the embedding; Metering is to concentrate the control requirement that whether reaches total amount in order to check, and prepares for the used bottle quantity of embedding; Embedding is the final step of specific yeast liquid being made the finished product main technique. This technique is to realize according to the step shown in this accompanying drawing 11: 11.1 special sexupara mixed liquors after will concentrating are transported to the cooling shown in this accompanying drawing 11 but

In the tank of machine (11A), be cooled to 12-15 ℃, 11.2 will through the mixed liquor of cooler tank cooling, be transported in the tank of dosing machine (11B)

Metering is transported to filling and sealing machine (11C) with the mixed liquor after the metering and carries out embedding, system

Become finished product (11D). Become oral or gavage the biological immunomodulator that ox is used that is mainly of liquid form, be used for giving anti-and cure mad cattle disease. The microbe species (showing listed microorganism but be not limited only to this) that table 3. this patent is related

The affiliated class-01 that belongs to	Saccharomyces cerevisiae Hansen saccharomyces cerevisiae
					Former purposes-01	Wine brewing, edible and food manufacturing
ACCC2034	ACCC2035	ACCC2036	ACCC2037	ACCC2038
					ACCC2039	ACCC2040	ACCC2041	ACCC2042	AS2.1
AS2.4	AS2.11	AS2.14	AS2.16	AS2.56
					AS2.69	AS2.70	AS2.93	AS2.98	AS2.101
AS2.109	AS2.110	AS2.112	AS2.139	AS2.173
					AS2.174	AS2.182	AS2.196	AS2.242	AS2.336
AS2.346	AS2.369	AS2.374	AS2.375	AS2.379
					AS2.380	AS2.382	AS2.390	AS2.393	AS2.395
AS2.396	AS2.397	AS2.398	AS2.399	AS2.400
					AS2.406	AS2.408	AS2.409	AS2.413	AS2.414
AS2.415	AS2.416	AS2.422	AS2.423	AS2.430
					AS2.431	AS2.432	AS2.451	AS2.452	AS2.453
AS2.458	AS2.460	AS2.463	AS2.467	AS2.486
					AS2.501	AS2.502	AS2.503	AS2.504	AS2.516
AS2.535	AS2.536	AS2.558	AS2.560	AS2.561
					AS2.562	AS2.576	AS2.593	AS2.594	AS2.614
AS2.620	AS2.628	AS2.631	AS2.666	AS2.982
					AS2.1190	AS2.1364	AS2.1396	IFFI1001	IFFI1002
IFFI1005	IFFI1006	IFFI1008	IFFI1009	IFFI1010
					IFFI1012	IFFI1021	IFFI1027	IFFI1037	IFFI1042
IFFI1043	IFFI1045	IFFI1048	IFFI1049	IFFI1050

IFFI1052	IFFI1059	IFFI1060	IFFI1063	IFFI1202
					IFFI1203	IFFI1206	IFFI1209	IFFI1210	IFFI1211
IFFI1212	IFFI1213	IFFI1214	IFFI1215	IFFI1220
					IFFI1221	IFFI1224	IFFI1247	IFFI1248	IFFI1251
IFFI1270	IFFI1277	IFFI1287	IFFI1289	IFFI1290
					IFFI1291	IFFI1292	IFFI1293	IFFI1297	IFFI12300
IFFI1301	IFFI1302	IFFI1307	IFFI1308	IFFI1309
					IFFI1310	IFFI1311	IFFI1335	IFFI1336	IFFI1337
IFFI1338	IFFI1339	IFFI1340	IFFI1345	IFFI1348
					IFFI1396	IFFI1397	IFFI1399	IFFI1411	IFFI1413
The affiliated class-02 that belongs to	Saccharomyces cerevisiae Hansen Var.ellipsoideus (Hansen) Dekker ellipse brewing yeast
					Former purposes-02	Wine brewing, edible and food manufacturing
ACCC2043	AS2.2	AS2.3	AS2.8	AS2.53
					AS2.163	AS2.168	AS2.483	AS2.541	AS2.559
AS2.606	AS2.607	AS2.611	AS2.612
The affiliated class-03 that belongs to	Saccharomyces chevalieri Xue Guilliermond watt yeast
					Former purposes-03	Wine brewing, edible and food manufacturing
AS2.131	AS2.213
					The affiliated class-04 that belongs to	Saccharomyces delbrueckii Dare cloth yeast
Former purposes-04	Food fermentation
					AS2.285
The affiliated class-05 that belongs to	Saccharomyces delbrueckii Lindner ver.mongolicus (Saito) Lodder et van Rij Mongolia Dare cloth yeast
					Former purposes-05	Food fermentation
AS2.209	AS2.1157

The affiliated class-06 that belongs to	Saccharomyces exiguous Hansen saccharomyces exiguus
					Former purposes-06	Food fermentation
AS2.349	AS2.1158
					The affiliated class-07 that belongs to	Saccharomyces fermentati (Saito) Lodder et van Rij saccharomyces fermentati
Former purposes-07	Food fermentation
					AS2.286	AS2.343
The affiliated class-08 that belongs to	Saccharomyces Logos van laer et Denamur ex Jorgensen Lip river lattice yeast
					Former purposes-08	The functions such as class, food fermentation make grape wine
AS2.156	AS2.327	AS2.335
					The affiliated class-08 that belongs to	Saccharomyces mellis (Fabian et Quinet) Lodder et kreger van Rij saccharomyces mellis
Former purposes-08	With carbohydrate, starch based fermentation
					AS2.195
The affiliated class-09 that belongs to	The little saccharomyces ellipsoideus of Saccharomyces mellis Microellipsoides Osterwalder
					Former purposes-09	With carbohydrate, starch based fermentation
AS2.699
					The affiliated class-10 that belongs to	Saccharomyces oviformis Osteralder ellipsoideus yeast
Former purposes-10	With carbohydrate, starch based fermentation
					AS2.100
The affiliated class-11 that belongs to	Saccharomyces rosei (Guilliermond) Lodder et Kreger van Rij Luo Si yeast

Former purposes-11	With carbohydrate, starch based fermentation
					AS2.287
The affiliated class-12 that belongs to	Saccharomyces rouxii Boutroux Lu Shi yeast
					Former purposes-12	With carbohydrate, starch based fermentation, make edible paste, soy sauce etc.
AS2.178	AS2.180	AS2.370	AS2.371
					The affiliated class-13 that belongs to	Saccharomyces Sake Yabe saccharomyces sake
Former purposes-13	With carbohydrate, starch based fermentation
					ACCC2045
The affiliated class-14 that belongs to	Candida arborea Candida arborea
					Former purposes-14	Be used for feed, amino acids manufacturing, cellulose, starch, carbohydrate, protein fermentation etc.
AS2.566
					The affiliated class-15 that belongs to	Candida lambica (Lindner et Genoud) van.Uden et Buckley; Bright Bick Candida
Former purposes-15	Be used for producing ester under the high temperature, make flavoring essence etc.
					AS2.1182
The affiliated class-16 that belongs to	Candida Krusei (Castellani) Berkhout Cruise yeast
					Former purposes-16	Be used for wine brewing, make feed, amino acids, albumen etc.
AS2.1045
The affiliated class-17 that belongs to	Candida lipolytica (Harrison) Diddens et Lodder Candida lipolytica

Former purposes-17	Be used for oil dewaxing, make organic acid substance
					AS2.1207	AS2.1216	AS2.1220	AS2.1379	AS2.1398
AS2.1399	AS2.1400
					The affiliated class-17 that belongs to	The medium-sized level and smooth Candida of Candida parapsilosis (Ashford) Langeron et Talice Var. intermedia Van Rij et Verona
Former purposes-17	Utilize carbohydrate, starch based fermentation to make feed
					AS2.491
The affiliated class-18 that belongs to	Candida parapsilosis (Ashford) Langeron et Talice Candida parapsilosis
					Former purposes-18	Utilize pentose class hydrolyzate to make feed
AS2.590
					The affiliated class-19 that belongs to	Candida pulcherriman (Lindner) Windisch iron oxide red yeast
Former purposes-19	Stimulating growth
					AS2.492
The affiliated class-20 that belongs to	Candida rugosa (Anderson) Diddens et Lodder fold candida
					Former purposes-20	Oil dewaxing, production organic acid etc.
AS2.511	AS2.1367	AS2.1369	AS2.1372	AS2.1373
					AS2.1377	AS2.1378	AS2.1384
The affiliated class-21 that belongs to	Candida tropicalis (Castellani) Berkhout candida tropicalis
					Former purposes-21	Carbohydrate fermentation; Cellulose, hemicellulose fermentation; Paper pulp already ferments; Sulfurous acid tobacco fermentation; Feed is made; Yeast extract, ergosterol manufacturing etc.
ACCC2004	ACCC2005	ACCC2006	AS2.164	AS2.402
					AS2.564	AS2.565	AS2.567	AS2.568	AS2.617
AS2.637	AS2.1387	AS2.1397

The affiliated class-22 that belongs to	Candida utilis Henneberg Lodder et Kreger Van Rij candida utili
					Former purposes-22	Eat and the feed manufacturing
AS2.120	AS2.281	AS2.1180
					The affiliated class-23 that belongs to	The false capsule yeast of Crebrothecium ashbyii (Guilliermond) Routein=Eremothecium ashbyii Guilliermond A Shu
Former purposes-23	Be used for riboflavin manufacturing etc.
					AS2.481	AS2.482	AS2.1197
The affiliated class-24 that belongs to	Geotrichum candidum Link geotrichum candidum
					Former purposes-24	Feed is made;
ACCC2016	AS2.361	AS2.498	AS2.616	AS2.1035
					AS2.1062	AS2.1080	AS2.1132	AS2.1175	AS2.1183
					The affiliated class-25 that belongs to	Hansenula anomala (Hansen) Het Psydow Hansenula anomala
Former purposes-25	Spices is made; Improve drinks fragrance, food flavor etc.;
					ACCC2018	AS2.294	AS2.295	AS2.296	AS2.297
AS2.298	AS2.299	AS2.300	AS2.302	AS2.338
					AS2.339	AS2.340	AS2.341	AS2.470	AS2.592
AS2.641	AS2.642	AS2.782	AS2.635	AS2.794
					The affiliated class-26 that belongs to	Hansenula arabitolenes Fang arabite Hansenula yeast
Former purposes-26	Produce the pure and mild glycerine of arabinose;
					AS2.887
The affiliated class-27 that belongs to	The outstanding fourth Hansenula yeast of Hansenula jadinii (A.et R Sartory Weill et Meyer) Wickerham

Former purposes-27	Do not find the report of application
					ACCC2019
The affiliated class-28 that belongs to	Hansenula saturnus (Klocker) H et P sydow Saturn Hansenula yeast
					Former purposes-28	Do not find the report of application
ACCC2020
					The affiliated class-29 that belongs to	Hansenula schneggii (Weber) Dekker Amur Hansenula yeast
Former purposes-29	Do not find the report of application
					AS2.304
The affiliated class-30 that belongs to	The inferior film Hansenula yeast of Hansenula subpelliculosa Bedford
					Former purposes-30	From multiple liquor raw material processed or poor slag, obtain, but do not find the report of application
AS2.740	AS2.760	AS2.761	AS2.770	AS2.783
					AS2.790	AS2.798	AS2.866
The affiliated class-31 that belongs to	Kloeckera apiculata (Reess emend.Klocker) Janke lemon shape Ke Leke yeast
					Former purposes-31	Do not find the report of application
ACCC2022	ACCC2023	AS2.197	AS2.496	AS2.714
					ACCC2021	AS2.711
The affiliated class-32 that belongs to	Lipomycess starkeyi Lodder et van Rij saccharomyces oleaginosus
					Former purposes-32	Do not find the report of application
AS2.1390	ACCC2024
					The affiliated class-33 that belongs to	Pichia farinose (Lindner) Hansen pichia farinose
Former purposes-33	Do not find the report of application
					ACCC2025	ACCC2026	AS2.86	AS2.87	AS2.705

AS2.803
					The affiliated class-34 that belongs to	Pichia membranaefaciens Hansen Pichia membranaefaciens
Former purposes-34	Do not find the report of application
					ACCC2027	AS2.89	AS2.661	AS2.1039
The affiliated class-35 that belongs to	Rhodosporidium toruloides Banno spore yeast of red winter
					Former purposes-35	Do not find the report of application
ACCC2028
					The affiliated class-35 that belongs to	Rhodotorula glutinis (Fresenius) Harrison rhodotorula
Former purposes-35	Fermenting carbohydrate, fermentation protein class, manufacturing food, condiment etc.
					AS2.2029	AS2.280	ACCC2030	AS2.102	AS2.107
AS2.278	AS2.499	AS2.694	AS2.703	AS2.704
					AS2.1146
The affiliated class-36 that belongs to	The little rhodotorula of Rhodotorula minuta (Saito) Harrison
					Former purposes-36	Fermenting carbohydrate, fermentation protein class, manufacturing food, condiment etc.
AS2.277
					The affiliated class-37 that belongs to	Rhodotorula rubar (Demme) Lodder rhodothece rubra
Former purposes-37	Fermenting carbohydrate, fermentation protein class, manufacturing food, condiment, feed etc.
					AS2.21	AS2.22	AS2.103	AS2.105	AS2.108
AS2.140	AS2.166	AS2.167	AS2.272	AS2.279
					AS2.282	ACCC2031

The affiliated class-38 that belongs to	Saccharomyces carlsbergensis Hansen Ka Ersi yeast
					Former purposes-38	Make food, brew alcoholic beverages, feed etc.
AS2.113	ACCC2032	ACCC2033	AS2.312	AS2.116
					AS2.118	AS2.121	AS2.132	AS2.162	AS2.189
AS2.200	AS2.216	AS2.265	AS2.377	AS2.417
					AS2.420	AS2.440	AS2.441	AS2.443	AS2.444
AS2.459	AS2.595	AS2.605	AS2.638	AS2.742
					AS2.745	AS2.748	AS2.1042
The affiliated class-39 that belongs to	Saccharomyces uvarum Beijer saccharomyces uvarum
					Former purposes-39	Make food, brew alcoholic beverages, feed etc.
IFFI1023	IFFI1032	IFFI1036	IFFI1044	IFFI1072
					IFFI1205	IFFI1207
The affiliated class-40 that belongs to	Saccharomyces willianus Saccardo Weir yeast
					Former purposes-40	Make food, brew alcoholic beverages, feed etc.
AS2.5	AS2.7	AS2.119	AS2.152	AS2.293
					AS2.381	AS2.392	AS2.434	AS2.614	AS2.1189
					The affiliated class-41 that belongs to	Saccharomyces sp. saccharomycete
Former purposes-41	Make brandy etc.
					AS2.311
The affiliated class-42 that belongs to	Saccharomycodes ludwigii Hansen rood class yeast
					Former purposes-42	Do not see the report of application
ACCC2044	AS2.243	AS2.508

The affiliated class-43 that belongs to	Saccharomycodes sinenses Yue China class yeast
					Former purposes-43	Do not see the report of application
AS2.1395
					The affiliated class-44 that belongs to	Schizosaccharomyces octosporus Beijerinck eight spore fission yeasts
Former purposes-44	Do not see the report of application
					ACCC2046	AS2.1148
The affiliated class-45 that belongs to	Schizosaccharomyces pombe Lindner chestnut wine fission yeast
					Former purposes-45	Lactose fermenters, brew alcoholic beverages, feed etc.
ACCC2047	ACCC2048	AS2.214	AS2.248	AS2.249
					AS2.255	AS2.257	AS2.259	AS2.260	AS2.274
AS2.994	AS2.1043	AS2.1149	AS2.1178	IFFI1056
					The affiliated class-46 that belongs to	Sporobolomyces roseus Kluyver et van Niel shadow yeast
Former purposes-46	Lactose fermenters, brew alcoholic beverages, feed, antibiotic etc.
					ACCC2049	ACCC2050	AS2.19	AS2.962	AS2.1036
ACCC2051	AS2.261	AS2.262
					The affiliated class-47 that belongs to	Torulopsis Candida (Saito) Lodder Torulopsis candida
Former purposes-47
					AS2.270	ACCC2052
The affiliated class-48 that belongs to	The unknown torulopsis of Torulopsis famta (Harrisn) Lodder et van Rij
					Former purposes-48
ACCC2053	AS2.685

The affiliated class-49 that belongs to	Torulopsis globosa (Olson et Hammer) Lodder et van Rij torulopsis
					Former purposes-49
ACCC2054	AS2.202
					The affiliated class-50 that belongs to	The usual torulopsis of Torulopsis inconspicua Lodder et Kreger van Rij
Former purposes-50
					AS2.75
					The affiliated class-51 that belongs to	Trichosporon behrendii Lodder et Kreger van Rij shellfish thunder trichosporon cutaneum
Former purposes-51
					ACCC2056	AS2.1193
The affiliated class-52 that belongs to	Trichosporon capitatum Diddens et Lodder head trichosporon cutaneum
					Former purposes-52
ACCC2056	AS2.1385
					The affiliated class-53 that belongs to	Trichosporon cutaneum (de Beurm et al.) Ota trichosporon cutaneum
Former purposes-53
					ACCC2057	AS2.25	AS2.570	AS2.571	AS2.1374
The affiliated class-54 that belongs to	Wickerhamia fluorescens (Soneda) Soneda Brunswick yeast
					Former purposes-54
ACCC2058	AS2.1388

Table 4. specific yeast enlarges medium component table (in the 1000L nutrient solution)

Medium component	Quantity
		Haw liquid	200L
Fruit of Chinese magnoliavine liquid	200L
		Date liquid	200L
Soybean juice	200L
		Apple liquid	200L

Annotate: 1. in the table various liquid all be according to: the ratio of material/water=1/10 is processed into.

2. upper table nutrient solution will be adjusted in pH2.5 ± 0.2 scope.

Claims (12)

1. one kind is made into livestocks such as ox with specific immunologic function with the manufacture method of immunomodulator with saccharomycete, for giving anti-and curing various mad cattle diseases, it is characterized in that, adopt device and the production method of micro-alternating bio-electric field, adopt the characteristic radio ripple to activate the step of the latent function gene of every Yeasts, include:

1. gene activation device (2) is set,

2. according to one-tenth assignment system culture medium first (3) P of subordinate list one, and sterilization treatment,

3. select the saccharomyces cerevisiae of IFFI1021 yeast specie, according to IFFI1021 wine brewing cell/culture medium 〉=1 * 10 of living⁸Individual ratio is injected the culture medium first, will cultivate the gene activation case (23) that first (3) is put into gene activation device (2) again,

4. the temperature in the maintenance container was cultivated H1 hour in the T1 scope,

5. open electromagnetic wave transmitter (21), the output wave frequency be adjusted to give in the fixed F scope,

6. electric wave transmitter output electromagnetic field intensity is adjusted in the V1 scope,

7. the gene activation case (23) of the blake bottle of yeast IFFI1021 nutrient solution will be housed, be installed to the receiver amplifier output according to the pattern shown in the gene activation device (2), receive frequency is adjusted in the identical F scope with the tranmitting frequency of transmitter (24). The distance of regulating simultaneously between transmitter (24) and the receiving instrument (25) is L1,

8. under these conditions, and the temperature conditions of maintenance T1, activate H2 hour,

9. IFFI1021 yeast cells after above condition activates adopts vacuum refrigeration to make ampoule or make the pulvis preservation in dry method.

2. immunomodulator that the livestocks such as ox are used is used for giving anti-and cures various mad cattle diseases, it is characterized in that, is the injection agent that utilizes saccharomycete to adopt method described above to make, and orally gavages agent.

3. the livestock such as ox as claimed in claim 1 is characterized in that with the manufacture method of immunomodulator, adopts 4 kinds of different characteristic radio magnetic wave frequency F1, F2, F3, F4 can form and activate respectively human body B, T, K, 4 species specificity yeast of the albumen of the latent immunity gene of NK.

4. the domestic animal such as ox as claimed in claim 1 is held the manufacture method with immunomodulator, it is characterized in that, when characteristic frequency F1 is the 6000M-18000MHz scope, activate and be used for regulation and control B cellular immune function because of yeast, when characteristic frequency F2 is the 7000-19000MHz scope, activate and be used for the modulating T cell immunologic function because of yeast, when characteristic frequency F3 is the 8000M-17000MHz scope, activate and be used for regulation and control K cellular immune function because of yeast, when characteristic frequency F4 is the 8000M-16000MHz scope, activate for regulation and control NK cellular immune function because of yeast.

5. the livestock such as ox as claimed in claim 1 is with the manufacture method of immunomodulator, it is characterized in that, it is 37 ± 5 ℃ that container when adopting the characteristic radio ripple to activate saccharomycetic latent function gene keeps temperature T 1 scope, incubation time H1 scope when adopting the characteristic radio ripple to activate saccharomycetic latent function gene is 24-56 hour, electric wave transmitter output electromagnetic field unit strength scope is 230 ± 15Mv/cm, distance L 1 between electric wave transmitter and the receiving instrument is 100 ± 10cm, electric wave transmitter output electromagnetic field intensity V1 scope is 21.5 to 24.5 volts, saccharomycetic activationary time H2 is 42 to 72 hours during radiation when adopting the characteristic radio ripple to activate saccharomycetic latent function gene, after adopting the characteristic radio ripple to activate saccharomycetic latent function gene, adopt the mode of vacuum freeze drying to make ampulla or make pulvis and preserve, the quantity P of the culture medium first (3) of employing can be 1000ml or more than.

6. livestocks such as ox are with the oral agent manufacture method that gavages of immunomodulator, it is characterized in that, adopt following steps, first step, adopt the method for claim 1 to produce and activate the barms that is used for regulation and control immunologic function gene, second step, the saccharomycete that has activated the latent function gene is carried out the envirment factor domestication to be cultivated, third step, to cultivate through enlarging through the saccharomycete of envirment factor domestication, decide kind and mix concentratedly by giving, desired saccharomycete human immunity conditioning agent is namely made in modulation.

7. the livestock such as ox as claimed in claim 6 is with the oral manufacture method that gavages agent of immunomodulator, it is characterized in that, the saccharomycete that has activated the latent function gene is carried out the step that the envirment factor domestication is cultivated, include: 1. domesticating device (6) is set, 2. according to the good culture medium second of recipe configuration (7) of subordinate list two, and sterilization treatment, 3. get the bacterial classification that culture medium second 1000ml is injected in the domesticating device (6) and tame tank (26)

In, 4. get at every turn regulation and control certain cell a kind of specific yeast liquid 10ml (the yeast juice living cells contains

Amount 〉=1 * 10⁸Individual/as ml), to inject the bacterial classification of domesticating device (6) and tame tank (26), 5. open the wave generator (21) of domesticating device (6), and be adjusted to this kind of regulation and control cell and exempt from

On the specific yeast selectivity frequency F of epidemic disease gene, the electric wave output voltage of 6. regulating domesticating device (6) is that every 100ml culture medium is employed

Received-signal strength is 5-10v, 7. keeps above-mentioned wave frequency and intensity of wave constant, cultivates at 37 ± 5 ℃ temperature conditions

After 48-96 hour, separate under the condition that is kept at 0-4 ℃ for subsequent use.

8. the livestock such as ox as claimed in claim 6 is with the oral manufacture method that gavages agent of immunomodulator, it is characterized in that, to comprise following content through enlarging to cultivate to the step of making finished product through the saccharomycete of envirment factor domestication: 1. specific yeast is set enlarges culture apparatus (8), 2. prepare culture medium third, and after sterilization treatment, be injected into respectively culture tank 8A, 8B,

In the 8C tank, 3. will activate through manual simulation's life electric wave method, and through tolerating low PH (less than PH2.5)

The adjusting B that obtains of cultivation, T, K, the specificity ferment of NK cellular immune function

Mother, at every turn a kind of, be input to the conduct kind that specific yeast enlarges culture apparatus (8)

Among the culture tank A of sub-tank, as seed liquor, then will cultivate according to giving certainty ratio

Tank A tank seed liquor is injected into to enlarge in the culture medium of culture tank B tank and cultivates, and 4. regulates wave generator (21), and making it export biological electric wave is F, and simultaneously according to

0.5-1.0v/ the requirement that rises is calculated, and sets received-signal strength, 5. keeps in above-mentioned wave frequency and the constant situation of received-signal strength 37 ± 5 ℃ temperature strip

Cultivated 56-72 hour under the part, the specific yeast living cells of 6. regulating the cellular immunity gene in culture tank B tank reaches 20

During hundred million/ml, in B tank yeast juice input C tank, prepare to be transported to lower road and mix

Technique. And selected needed saccharomycetic kind, and various saccharomycetic mixing ratio are mixed as follows, and be concentrated, makes finished product, and mixing arrangement (9) is 1. set up in encapsulation, and 4 species specificity yeast juices are input to respectively each storage tank

9A, 9B, 9C among the 9D, 2. is input to blending tank with this species specificity yeast juice in each tank according to the ratio of equivalent

Mix among the M, 3. mixed yeast juice be injected in the concentrator (10), prepare concentrated, 4. in concentrated (10A) tank of the first order of concentrator (10) with the specific yeast mixed liquor,

Be concentrated into 80% (measurement basis calculation), then be transported in the second level thickener (10B) dense

Contracting. Concentrated can adopt that freezing vacuum is concentrated, the normal temperature partial vacuum is concentrated or heat concentrated etc.

Method, but which kind of concentrated mode all must take the work that keeps special yeast cells as

Benchmark 5. is concentrated into 80% again in second level thickener tank (10B), the specific yeast mixed liquor after 6. will concentrating is transported to the cooling tank of cooling packaging system

In, be cooled to 12-15 ℃, 7. will through the mixed liquor of cooling tank cooling, be transported in the measuring tank and measure, after will measuring

Mixed liquor be transported to embedding in the potting machine, make the oral agent finished product that gavages.

9. such as livestock immunomodulator manufacture methods such as claim 1 or 6 described oxen, it is characterized in that the barms that is suitable for is any bacterial classification in the table 3.

10. livestocks such as ox gavage agent with immunomodulator oral, are used for giving anti-and cure mad cattle disease, it is characterized in that it is manufactured by step claimed in claim 6.

11. the oral agent that gavages as claimed in claim 10 is characterized in that, the saccharomycete of its utilization can be the arbitrarily saccharomycete in the table 3.

12. such as livestock immunomodulators such as claim 1 or 10 described oxen, it is characterized in that, it can contain in the following specific yeast bacterium any one, any two kinds, any three kinds, any four kinds, namely regulate the specific yeast bacterium of B cellular immunity gene, namely regulate the specific yeast bacterium of T cellular immunity gene, namely regulate the specific yeast bacterium of K cellular immunity gene, namely regulate the specific yeast bacterium of NK cellular immunity gene.

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