CN1372971A - Chinese-medicinal injection for treating cancer - Google Patents
Chinese-medicinal injection for treating cancer Download PDFInfo
- Publication number
- CN1372971A CN1372971A CN 02114987 CN02114987A CN1372971A CN 1372971 A CN1372971 A CN 1372971A CN 02114987 CN02114987 CN 02114987 CN 02114987 A CN02114987 A CN 02114987A CN 1372971 A CN1372971 A CN 1372971A
- Authority
- CN
- China
- Prior art keywords
- add
- ethanol
- liquid
- injection
- cancer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
A Chinese medicine in the form of injection for treating lung cancer, esophageal cancer, stomach cancer, liver cancer and cervical cancer is prepared from the Chinese-medicinal material "Tongguanteng" through reflux extraction in alcohol, filtering, concentrating filtrate, taking supernatant, extracting in ethyl acetate, recovering thick extract, diluting, ultrafiltering, spray drying to obtain total glycoside; soaking the residue, decocting, vacuum concentrating, adding alcohol, filtering, deposition, washing, centrifugal separation, spray drying to obtain extract containing polyose; mixing said total glycoside with said extract, adding water for injection and activated carbon, filtering, regulating pH value, filtering and sterilizing.
Description
Affiliated technical field
The present invention relates to a kind of medicine for the treatment of cancer, specifically relate to a kind of Chinese medicine injection for the treatment of cancer.
Background technology
At present, cancer has become after cardiovascular and cerebrovascular disease, and the second largest killer of harm humans health shows that according to interrelated data the annual whole world has 7,000,000 people to die from cancer approximately.Cancer has been a kind of frequently-occurring disease, and with regard to present medical science level, the whole healings of cancer patient still are difficult to accomplish, but the generation of control cancer can be accomplished with the reduction mortality rate.Chinese medicine is of long standing and well established to the understanding of tumor.Aspect morbidity, various tumors due to illness machine are not quite similar, and say on the whole, think it mainly is positive QI-insufficiency, evil poison is taken advantage of a weak point, and causes the stagnation of QI, blood stasis, expectorant and hands over knot with fixed attention mutually, become lump for a long time and gradually, and the pathogen collection of crouching, the circulation of inevitable further retardance qi-blood-body fluid, consume vital essence, corrode the internal organs meridians, cause and effect links to each other, and deteriorated case is grown thickly, final threat to life.The traditional Chinese medicine of China has accumulated many valuable experience on tumor diagnosis and treatment.In fact, traditional Chinese medical science practice in several thousand is just as be equivalent to the once Preliminary screening of the large-scale cancer therapy drug that carries out from natural plants, animal, mineral and marine organisms, the Chinese medicine that these Preliminary screening are come out relends and helps modern technologies to carry out explication de texte, simulation modification and pharmacology and effect research, might develop some brand-new effective antitumor medicines fully, make the traditional Chinese medical science make bigger contribution the mankind's antitumor cause.The determination of treatment based on pathogenesis obtained through differentiation of symptoms and signs of the traditional Chinese medical science, treating both the principal and secondary aspects of a disease, equilibrium between yin and yang scheduling theory and method if can be fused in the Comprehensive Treatment of doctor trained in Western medicine to tumor rightly, also will promote the common raising of the traditional Chinese medical science and oncotherapy.
Summary of the invention
The object of the present invention is to provide a kind of Chinese medicine injection that multiple malignant tumor such as pulmonary carcinoma, the esophageal carcinoma, gastric cancer, hepatocarcinoma, cervical cancer is all had good efficacy.
The Chinese medicine injection of treatment cancer provided by the invention, this injection is prepared by following method:
(1) with the Caulis Marsdeniae Tenacissimae is raw material, add 8~12 times of amount 70~90% ethanol, merceration 0.5~24h refluxes 1~5 time, filters, medicinal residues are standby, merge alcohol extract, being evaporated to relative density is 1.08~1.18, leaves standstill, get clear liquid, ethyl acetate with 5~10 times of amounts divides 5~10 extractions, merges ethyl acetate extraction liquid, reclaims ethyl acetate to most, get thick paste, thin up leaves standstill, and gets the clear liquid molecular cut off and be 50000 and 3000 membrane ultrafiltration, ultrafiltrate is evaporated to relative density 1.10~1.15, and spray drying gets total glycosides extractive;
(2) medicinal residues add the water logging bubble of 6~8 times of amounts, decoct 2~4 times, merge decocting liquid, be evaporated to relative density 1.08~1.20, add ethanol and make and contain the alcohol amount and reach 20~25%, left standstill 12~72 hours, filter, filtrate adds ethanol precipitation again to be made and contains alcohol amount and reach 60~90%, left standstill 24~72 hours, and filtered, it is colourless that precipitation is washed till filtrate with ethanol, the water heating for dissolving that adds 5~7 times of amounts, add tannic acid liquid to nothing and precipitate till the generation, centrifugal, it is 1.15~1.20 that centrifugal liquid is evaporated to relative density, adding ethanol makes and contains alcohol amount and reach 70~80%, left standstill 24~96 hours, and filtered, precipitation is washed till no tannic acid reaction with ethanol, spray drying must contain the extract of polysaccharide;
(3) above-mentioned two kinds of extracts are mixed, add the injection water, after the heated and boiled, add active carbon, continue to boil, filtered while hot adds NaHSO in the filtrate
3, EDTA-Na
2, NaCl, propylene glycol is transferred PH to 7.0 with NaOH liquid, uses filtering with microporous membrane, gets finished fluid, embedding, sterilization, promptly.
Chinese medicine injection of the present invention has to eliminate the phlegm invigorates blood circulation, and the effect of blood stasis-eliminating and stagnation-dissipating is mainly used in the treatment of pulmonary carcinoma, also can be used for esophageal carcinoma, gastric cancer, and hepatocarcinoma, the treatment of multiple malignant tumor such as cervical cancer, curative effect is sure, have no side effect.
Below in conjunction with embodiment and pharmacodynamics test Chinese medicine injection of the present invention is further described.
Embodiment 1
Take by weighing Caulis Marsdeniae Tenacissimae 1200g, add 10 times of amount 85% ethanol, merceration 4h refluxes 4 times, 2h/ time, filter, medicinal residues are standby, merge alcohol extract, being evaporated to relative density is 1.14 (60 ℃), leave standstill, get clear liquid, divide 7 extractions with the ethyl acetate of 7 times of amounts, merge ethyl acetate extraction liquid, reclaim ethyl acetate to the greatest extent, get thick paste, thin up is to 1000ml, leave standstill, get the clear liquid molecular cut off and be 50000 and 3000 membrane ultrafiltration, ultrafiltrate is evaporated to relative density 1.15 (60 ℃), and spray drying gets total glycosides extractive.Medicinal residues add the water logging of 10 times of amounts and steeped 3 hours, decoct 4 times, each 1.5 hours, merge decocting liquid, be evaporated to relative density 1.20 (60 ℃), adding ethanol makes and contains the alcohol amount and reach 20%, left standstill 48 hours, and filtered, filtrate adds ethanol precipitation again to be made and contains the alcohol amount and reach 80%, left standstill 48 hours, filter, it is colourless that precipitation is washed till filtrate with 80% ethanol, adds the water heating for dissolving of 7 times of amounts, adding 20% tannic acid liquid to nothing precipitates till the generation, centrifugal, rotating speed is 20000 rev/mins, and it is 1.15 (60 ℃) that centrifugal liquid is evaporated to relative density, adding ethanol makes and contains alcohol amount and reach 80%, left standstill 72 hours, and filtered, precipitation is washed till no tannic acid reaction with 80% ethanol, spray drying must contain the extract of polysaccharide.Above-mentioned two kinds of extracts are mixed, add 900ml water for injection, after the heated and boiled, add 0.2% active carbon, continue to boil 30min, filtered while hot.Add NaHSO in the filtrate
32g, EDTA-Na
20.2g, NaCl7.07g, propylene glycol 120g transfers PH to 7.0 with 40%NaOH liquid.Use the 0.22um filtering with microporous membrane, get finished fluid, embedding, sterilization, promptly.Embodiment 2
Take by weighing Caulis Marsdeniae Tenacissimae 1000g, add 8 times of amount 90% ethanol, merceration 4h refluxes 3 times, 1h/ time, filter, medicinal residues are standby, merge alcohol extract, being evaporated to relative density is 1.10 (60 ℃), leave standstill, get clear liquid, divide 5 extractions with the ethyl acetate of 5 times of amounts, merge ethyl acetate extraction liquid, reclaim ethyl acetate to the greatest extent, get thick paste, thin up is to 1000ml, leave standstill, get the clear liquid molecular cut off and be 50000 and 3000 membrane ultrafiltration, ultrafiltrate is evaporated to relative density 1.13 (60 ℃), and spray drying gets total glycosides extractive.Medicinal residues add the water logging of 8 times of amounts and steeped 1 hour, decoct 3 times, each 1 hour, merge decocting liquid, be evaporated to relative density 1.10 (60 ℃), adding ethanol makes and contains the alcohol amount and reach 25%, left standstill 24 hours, and filtered, filtrate adds ethanol precipitation again to be made and contains the alcohol amount and reach 70%, left standstill 24 hours, filter, it is colourless that precipitation is washed till filtrate with 70% ethanol, adds the water heating for dissolving of 5 times of amounts, adding 20% tannic acid liquid to nothing precipitates till the generation, centrifugal, rotating speed is 16000 rev/mins, and it is 120 (60 ℃) that centrifugal liquid is evaporated to relative density, adding ethanol makes and contains alcohol amount and reach 70%, left standstill 24 hours, and filtered, precipitation is washed till no tannic acid reaction with 70% ethanol, spray drying must contain the extract of polysaccharide.Above-mentioned two kinds of extracts are mixed, add 900ml water for injection, after the heated and boiled, add 0.2% active carbon, continue to boil 30min, filtered while hot.Add NaHSO in the filtrate
32g, EDTA-Na
20.2g, NaCl 7.07g, propylene glycol 120g transfers pH to 7.0 with 40%NaOH liquid.Use the 0.22um filtering with microporous membrane, get finished fluid, embedding, sterilization, promptly.Pharmacodynamics test
Adopt under the common light microscopic photography and mtt assay observe and detected the injection of 5 kinds of concentration of the present invention (20.5,25.6,32.0,40.0, the influence of 50.0mg crude drug/ml) human large intestine cancer Lovo cell, human hepatoma HepG2 cell, QGY-7703 cell, Hep3B cell and people's gastric cancer SGC-7901 cell of In vitro culture are grown, simultaneously, also detected the time-effect relationship of Caulis Marsdeniae Tenacissimae injection to the HepG2 cyto-inhibition of In vitro culture, and with ad pro injection and 5-Fu as positive control.The result shows, injection 20.5 to 50.0mg crude drug/ml of the present invention Lovo cell and SGC-7901 cell inhibitory rate are respectively 11.1% are increased to 50.9% and 7.5% to 43.3%, but the entire amount effect relationship is not obvious; To HepG2 cell inhibiting effect from 11.2% to 59.6%, and show certain dose-effect relationship on the whole; Similar to effect of QGY-7703 cell inhibiting and HepG2 cell; Except that individual concentrations suppression ratio>30%, injection of the present invention is obviously low than other hepatoma carcinoma cell to the Hep3B cyto-inhibition, and dose-effect relationship is not obvious, and this may be relevant with this cell disappearance antioncogene P53.When mainly showing as low concentration, the influence of pair cell form do not have obvious influence, but along with concentration increases, it is round that cellular morphology becomes gradually, cell density reduces, disperse each other, the cell transparency weakens, visible particle in the cell that has, but QGY-7703, Hep3B and SGC-7901 cellular morphology are not had obvious influence.Time-effect relationship shows, the Caulis Marsdeniae Tenacissimae injection promptly showed inhibitory action in first day, suppression ratio is 16.5~28.0%, inhibitory action further strengthened in second day, reach 32.9~67.8%, inhibitory action reaches the peak, about 67.8~71.6% during to the 3rd day, performance still in the 4th day has 28.6~34.1% suppression ratio, suppresses to drop to minimum on the 5th day.Conclusion: the Caulis Marsdeniae Tenacissimae injection has the obvious suppression effect to human liver cancer cell, stomach cancer cell and the colorectal cancer cells of In vitro culture, and inhibiting part mechanism may suppress oncogene P53 with enhancing and express relevant; Time-effect relationship shows, Caulis Marsdeniae Tenacissimae injection sustainable 4 days to the inhibitory action of tumor, and wherein, inhibitory action was the strongest in the 3rd day, almost disappeared to the 5th day inhibitory action.
Claims (1)
1, a kind of Chinese medicine injection for the treatment of cancer, this injection is prepared by following method:
(1) with the Caulis Marsdeniae Tenacissimae is raw material, add 8~12 times of amount 70~90% ethanol, merceration 0.5~24h refluxes 1~5 time, filters, medicinal residues are standby, merge alcohol extract, being evaporated to relative density is 1.08~1.18, leaves standstill, get clear liquid, ethyl acetate with 5~10 times of amounts divides 5~10 extractions, merges ethyl acetate extraction liquid, reclaims ethyl acetate to most, get thick paste, thin up leaves standstill, and gets the clear liquid molecular cut off and be 50000 and 3000 membrane ultrafiltration, ultrafiltrate is evaporated to relative density 1.10~1.15, and spray drying gets total glycosides extractive;
(2) medicinal residues add the water logging bubble of 6~8 times of amounts, decoct 2~4 times, merge decocting liquid, be evaporated to relative density 1.08~1.20, add ethanol and make and contain the alcohol amount and reach 20~25%, left standstill 12~72 hours, filter, filtrate adds ethanol precipitation again to be made and contains alcohol amount and reach 60~90%, left standstill 24~72 hours, and filtered, it is colourless that precipitation is washed till filtrate with ethanol, the water heating for dissolving that adds 5~7 times of amounts, add tannic acid liquid to nothing and precipitate till the generation, centrifugal, it is 1.15~1.20 that centrifugal liquid is evaporated to relative density, adding ethanol makes and contains alcohol amount and reach 70~80%, left standstill 24~96 hours, and filtered, precipitation is washed till no tannic acid reaction with ethanol, spray drying must contain the extract of polysaccharide;
(3) above-mentioned two kinds of extracts are mixed, add the injection water, after the heated and boiled, add active carbon, continue to boil, filtered while hot adds NaHSO in the filtrate
3, EDTA-Na
2, NaCl, propylene glycol is transferred PH to 7.0 with NaOH liquid, uses filtering with microporous membrane, gets finished fluid, embedding, sterilization, promptly.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB021149879A CN1137721C (en) | 2002-03-21 | 2002-03-21 | Chinese-medicinal injection for treating cancer |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB021149879A CN1137721C (en) | 2002-03-21 | 2002-03-21 | Chinese-medicinal injection for treating cancer |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1372971A true CN1372971A (en) | 2002-10-09 |
CN1137721C CN1137721C (en) | 2004-02-11 |
Family
ID=4743393
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB021149879A Expired - Fee Related CN1137721C (en) | 2002-03-21 | 2002-03-21 | Chinese-medicinal injection for treating cancer |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN1137721C (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101073564B (en) * | 2006-05-19 | 2010-10-13 | 山东轩竹医药科技有限公司 | Medicinal composition containing L-glutamine |
CN102028726A (en) * | 2010-12-22 | 2011-04-27 | 中国人民解放军第三○二医院 | Extract of Chinese herb marsdenia tenacissima as well as preparation method and application thereof |
CN1954845B (en) * | 2005-10-27 | 2012-03-07 | 山东轩竹医药科技有限公司 | Sarcandra compound medical composite and its preparation method |
CN104840499A (en) * | 2015-04-21 | 2015-08-19 | 四川国康药业有限公司 | Xiaoaiping tablet and preparation method thereof |
CN105193710A (en) * | 2015-08-07 | 2015-12-30 | 杨凌科森生物制药有限责任公司 | Preparation of Xiaoaiping oral solution |
-
2002
- 2002-03-21 CN CNB021149879A patent/CN1137721C/en not_active Expired - Fee Related
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1954845B (en) * | 2005-10-27 | 2012-03-07 | 山东轩竹医药科技有限公司 | Sarcandra compound medical composite and its preparation method |
CN101073564B (en) * | 2006-05-19 | 2010-10-13 | 山东轩竹医药科技有限公司 | Medicinal composition containing L-glutamine |
CN102028726A (en) * | 2010-12-22 | 2011-04-27 | 中国人民解放军第三○二医院 | Extract of Chinese herb marsdenia tenacissima as well as preparation method and application thereof |
CN104840499A (en) * | 2015-04-21 | 2015-08-19 | 四川国康药业有限公司 | Xiaoaiping tablet and preparation method thereof |
CN104840499B (en) * | 2015-04-21 | 2019-01-11 | 四川国康药业有限公司 | A kind of XIAOAIPING PIAN and preparation method thereof |
CN105193710A (en) * | 2015-08-07 | 2015-12-30 | 杨凌科森生物制药有限责任公司 | Preparation of Xiaoaiping oral solution |
Also Published As
Publication number | Publication date |
---|---|
CN1137721C (en) | 2004-02-11 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103768534B (en) | A kind of Chinese medicine composition with antitumor activity | |
CN104263790B (en) | A kind of saline cistanche polypeptide extract, preparation method and its usage | |
CN101904974B (en) | Chinese medicinal composition for treating malignant tumor diseases and preparation method thereof | |
CN1137721C (en) | Chinese-medicinal injection for treating cancer | |
CN103977206A (en) | Traditional Chinese medicine preparation capable of improving gastrointestinal digestive function and production method thereof | |
CN103142793A (en) | Traditional Chinese medicine formula for treating infantile diarrhea | |
CN104826012A (en) | Traditional Chinese medicine preparation for pituitary tumor surgery postoperative nursing and preparation method thereof | |
CN108936675A (en) | A kind of health food and preparation method thereof with treatment gout effect | |
CN103330781A (en) | Traditional Chinese medicine composite with anti-tumor effect and preparation method of injection of same | |
CN109275751A (en) | Folium cortex eucommiae blood-pressure decreasing tea and preparation method thereof | |
CN1692938A (en) | Compound traditional Chinese medicine for improving eyesight and its prepn. method | |
CN103585206B (en) | A kind of preparation method of Chrysosplenium extract and application thereof | |
CN102138957A (en) | Colocynth extract, and production method and application thereof | |
CN108635560A (en) | A kind of Chinese medicine and preparation method thereof for treating lymthoma | |
CN104188007B (en) | Bamboo-shoot beverage and preparation method thereof | |
CN114848786B (en) | Anticancer and immunity-increasing compound three-ginseng decoction | |
CN1119157C (en) | Cough and asthma relieving and phlegm eliminating capsule and its preparation | |
CN101015680B (en) | Use of F-Glucoside peptide in preparing medicine for treating tumour | |
CN1895503A (en) | Hairy antler capsules | |
CN106177416A (en) | A kind of traditional Chinese medicinal composition with effect of reducing blood sugar and preparation method thereof | |
CN106539981A (en) | A kind of complex polysaccharide of anti-curing oncoma and preparation method thereof | |
CN102048805A (en) | Chinese medicinal preparation for treating malignant tumor and preparation method thereof | |
CN102824605B (en) | A kind of Chinese medicine for the treatment of gastric cancer | |
CN108728314A (en) | A kind of SOD white wine preparation methods for supporting kidney function effect with protect liver | |
CN115245554A (en) | Poria cocos medicinal composition with stomach invigorating and spleen eliminating effects and application thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C19 | Lapse of patent right due to non-payment of the annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |