CN1369010A - 影响程序化细胞死亡的成分及其在林业植物发育改良中的应用 - Google Patents
影响程序化细胞死亡的成分及其在林业植物发育改良中的应用 Download PDFInfo
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Abstract
本发明提供了一种新的分离的多核苷酸,其与程序化细胞死亡和多种细胞发育机制相关,以及包含这种序列的基因构建物。用于调节森林植物的含量,结构,和代谢,特别是调节程序化细胞死亡和多种细胞发育机制的方法,该方法包括将一个或多个本发明的多核苷酸或基因构建物掺入森林植物的染色体。
Description
发明领域
本发明涉及植物发育反应的修饰。具体地,本发明与影响程序化细胞死亡的多核苷酸和多肽有关。可以将这些多核苷酸和多肽及含有这些多核苷酸和多肽的遗传构建体用于调节程序化细胞死亡,并因此改变林业植物细胞的发育循环,从而改变植物的发育。
发明背景
程序化细胞死亡(PCD)是主动过程,其中基因表达与导致细胞死亡的事件密切相关。植物生命循环有许多这些细胞死亡的例子。在植物繁殖和早期胚胎发育过程中,事件,例如在单性花发育时器官的脱落、花粉发育时绒毛毡层的变性和胚胎发育时胚柄的变性的都参与主动细胞死亡过程。在植物器官发生和成熟过程中,糊粉细胞降解、木质部中导管元件分化的终末期、在一些植物中的(例如,genusMonstera)叶片发育、叶/器官衰老、根冠细胞分化和植物/病原体相互作用中的高敏反应也是在植物发育循环中细胞死亡程序作用的例子。
到目前为止,大多数关于程序化细胞死亡的科学研究涉及哺乳动物细胞中的PCD。明显的形态学特点,例如细胞质凝聚、膜出泡、DNA断裂、细胞核的凝聚和断裂、及最终细胞残片吞噬证实PCD在这些细胞中的存在。在哺乳动物细胞中,PCD提供去除多余细胞和去除病原体或病原体感染细胞的机制。也相信正常PCD机制中的故障在诸多疾病状态,包括许多肿瘤中起重要作用。
还未广泛研究PCD在植物系统的作用。植物和哺乳动物PCD机制的初步比较提示在此机制中的一些相似性。可能的相似性有:需氧量;过氧化氢激活;在主动过程中钙的作用;转录条件;去磷酸化条件;蛋白分解和核分解酶的参与及细胞的凝聚和皱缩。在任何一个或多个区域中PCD机制的改变可影响植物的发育。
发明简述
简要地说,本发明提供分离的多肽,和编码该分离多肽的多核苷酸,该多肽在林业植物物种PCD途径和多种发育途径中具有活性。还提供含有该多核苷酸的遗传构建体和应用该遗传构建体调节林业植物中PCD和多种发育途径的方法。还提供掺入该遗传构建体及与野生型植物相比表现本发明多核苷酸和/或多肽改良内容的转基因细胞和植物。阐述应用本发明多核苷酸和/或多肽调节植物细胞死亡及调节多种林业植物物种发育途径的方法。
在哺乳动物PCD中,细胞循环通道的调节仿佛很重要,并提示细胞循环关卡调节物(cell cycle checkpoint regulators)可参与细胞死亡过程。例如,已知的肿瘤抑制物p45能介导细胞循环阻滞并能诱发PCD。视网膜母细胞瘤基因(RB)是参与p45介导反应的关键基因之一。此肿瘤抑制物能结合并抑制启动进入细胞循环的转录因子。此外,RB依赖其磷酸化状态在细胞死亡过程中起调节作用。RB蛋白水解的磷酸化状态调节和之后细胞中的RB水平对于细胞结局的判定是重要的。已经从林业物种中分离出编码视网膜母细胞瘤-相关多肽(SEQ ID NOS:36、37)的两个多核苷酸。将该多核苷酸编码的视网膜母细胞瘤-相关多肽确定为SEQ ID NOS:80、81。
另一个肿瘤抑制物基因,抑制素(prohibitin),也能阻滞细胞循环。已提示在大鼠B淋巴细胞中抑制素与膜-结合IgM的结合体为这些细胞中PCD的调节子。而且,在酵母菌中,抑制素同族物的缺失导致复制生命周期缩短,后者引起细胞循环时间的连续下降、衰老和细胞衰老。既然已在非-植物系统中进行了上述研究,在植物系统中(发现)有效的相似细胞循环调节子是可能的。已经从林业物种中分离出几个编码抑制素-相关多肽(SEQ ID NOS:22-26)的多核苷酸。将该多核苷酸编码的抑制素-相关多肽确定为SEQ ID NOS:67-71。
具有细胞持家功能的多核苷酸对于细胞的健康和生存是必须的,它们的缺失可导致细胞死亡。最早在温度-敏感突变体仓鼠细胞系中识别的一个此多核苷酸是DAD1(细胞死亡防御子1,DefenderAgainst Cell Death 1)。温度-敏感的突变体仓鼠细胞系中的细胞在限制性温度下发生PCD,并且显示Arabidopsis DAD1能挽救该仓鼠温度-敏感突变体。DAD1的存在也能挽救蠕虫新小杆线虫(Caenorhabditis elegans)发育胚胎中的细胞死亡,其发生发育-调节细胞死亡。DAD1已知为寡糖转移酶的成分,参与N-连接糖基化作用。由DAD1灭活诱导细胞死亡和在发育时期DAD1降低PCD的能力表明此持家基因的基本作用。已经从林业物种中分离出几个编码DAD1-相关多肽的多核苷酸(SEQ ID NOS:6-9)。将该多核苷酸编码的DAD1-相关多肽确定为SEQ ID NOS:51-54。
其它用于控制细胞生存和细胞死亡的持家多核苷酸是TATA盒结合蛋白(TFIID)。TFIID是RNA聚合酶基因转录所需的最主要的共同因子。TFIID与该TATA盒结合并参与转录因子组合的第一步,后者对于基因表达的控制是重要的。进化或组织特异地敲除TFIID活性的能力提供特异地诱导细胞死亡的方法。尚未报道关于植物TFIID敲除。已经从林业物种中分离出编码TFIID-相关转录起始因子的多核苷酸(SEQ ID NOS:41、42)。将该多核苷酸编码的TFIID相关转录起始因子确定为SEQ ID NOS:85、86。
参与控制哺乳动物细胞死亡的其它转录因子是pur-alpha。pur-alpha是单链DNA结合蛋白,已发现其在DNA复制和转录调控中起作用。pur-alpha通过两个机制能抑制哺乳动物细胞的PCD。第一个是阻抑Fas(CD-95)的转录,Fas是通过与其配体相互作用传导细胞死亡信号的受体,第二个是保护哺乳动物细胞免于p53介导的细胞死亡。已经从林业物种中分离出编码植物pur-alpha等位变体的多核苷酸(SEQ ID NOS:90-91)。将该多核苷酸编码的pur-alpha多肽的相应氨基酸序列确定为SEQ ID NOS:141-142。
细胞死亡的实际过程包括蛋白酶和核酸酶介导的蛋白质和核酸的降解。哺乳动物系统的实验工作提示蛋白酶是重要的细胞死亡的诱发剂。在动物中,半胱氨酸蛋白酶的caspase家族是此过程的主要效应剂。在植物中已识别出半胱氨酸蛋白酶,随糊粉粒和导管元件细胞死亡上调并与之特异性相关。已经将在林业物种中编码半胱氨酸蛋白酶的多核苷酸确定为SEQ ID NOS:92-125。将该多核苷酸编码多肽的相应的氨基酸序列确定为SEQ ID NOS:143-176。此外,已发现天冬氨酸核酸酶,nucellin与珠心细胞死亡特异相关。已经从植物物种中分离出编码nucellin类天冬氨酸核酸酶的多核苷酸(SEQ ID NOS:15-16)。将该多核苷酸编码的天冬氨酸核酸酶的相应的氨基酸序列确定为SEQ ID NOS:60-61。
除了实际的蛋白酶活性,在PCD时,通过遍在蛋白-proteosome途径分解性降解的蛋白质的指标是上调的。与果蝇SINA(Seven InAbsentia)基因的人类同源体在PCD时是激活的。在人类和果蝇中,已发现SINA以ubiquitination和降解特异蛋白质为目标。已经从林业物种中分离出编码SINA-相关多肽的多核苷酸(SEQ ID NOS:38-40和200)。将该多核苷酸编码的SINA-相关多肽确定为SEQ ID NOS:82-84和201。
核DNA分裂、核断裂和RNA降解是发生于动物和植物的PCD中的主动过程。在植物糊粉细胞、导管元件、对病原体发生高敏反应的细胞PCD时和在盐应激-诱发的细胞死亡时,已检测出特异性的植物脱氧核糖核酸酶和核糖核酸酶。已经从林业物种中分离出编码植物脱氧核糖核酸酶(SEQ ID NO:10)和木纤维性(xylogenic)核糖核酸酶(SEQ ID NO:45)的多核苷酸。将该多核苷酸编码的脱氧核糖核酸酶和核糖核酸酶的相应氨基酸序列分别确定为SEQ ID NOS:55和89。
在哺乳动物系统中,蛋白质例如Bcl-2的抑制caspase激活,其保护细胞免于死亡。但是,Bcl-2家族的其它成员,如Bax,因为它们能与Bcl-2相互作用并抑制其保护功能,而拮抗Bcl-2的保护作用和促进细胞死亡。发现最近发现的基因,BI-1(Bax抑制剂-1)抑制Bax-诱导的细胞死亡。此基因与早先识别的命名为TEGT的人类基因(睾丸增加的基因转录物)完全相同。将编码从林业物种分离的TEGT多肽的多核苷酸确定为SEQ ID NOS:43-44。将该多核苷酸编码的TEGT多肽的相应的氨基酸序列确定为SEQ OD NOS:87-88。
其它参与抑制PCD的蛋白质是BAG-1(Bcl-2-相关-athanoGene),阻断凋亡的多功能蛋白质,并在哺乳动物细胞中与包括Bcl-2家族蛋白质、激酶Raf-1、某些酪氨酸激酶生长因子受体和类固醇激素受体在内的多物种型蛋白质相互作用。其与激素-受体结合蛋白质RAP46相同。BAG-1与抗-凋亡蛋白质Bcl-2结合并使其产生效应,使细胞更耐受凋亡。人类BAG-1在人类白血病、结肠、子宫颈、乳腺、前列腺和肺癌细胞系中是高表达的。将编码从林业物种中分离的BAG-1多肽的多核苷酸确定为SEQ ID NO:204。将该多核苷酸编码的BAG-1多肽的相应氨基酸序列确定为:SEQ ID NO:205。此分离的编码BAG-1的多核苷酸含有遍在蛋白类结构域的Prosite基序,在人和鼠BAG-1蛋白质中也存在遍在蛋白类结构域。
许多哺乳动物系统的研究表明诱导PCD的处理也引起氧化应激,提示氧化应激在PCD中的作用。观察已经证实ROS(活性氧簇)的增加或细胞抗氧化剂的缺失能导致PCD。PCD可伴随着ROS的诱导,并且通过增加具有抗氧化特性的化合物可以阻抑PCD。活性氧类如超氧化物,羟基残基和过氧化氢可以结合细胞大分子并损害之。而且,它们可以启动链反应,其中自由基从一个分子移向另一个,导致广泛的细胞损伤和毒性。
植物在PCD时,如在渗透压介导的死亡、高敏反应及导管元件分化终末期时也表现ROS诱导。在动物细胞中,结合NADPH叶绿醇复合体的膜导致超氧化物的产生,然后该超氧化物转换成其它的ROS。另外,叶绿醇的激活需要小胞浆蛋白质rac2。当向小鼠插入组成性活性rac2突变体时,与野生型小鼠相比,出现PCD的明显增加。生物化学和免疫化学研究表明NADPH叶绿醇和rac2存在于植物细胞中并在高敏性PCD时相互作用。而且,在渗透压介导的细胞死亡时和在导管元件分化终末期,该NADPH叶绿醇是有活性的。已经从水稻和arabidopsis中克隆了该gp 91 NADPH叶绿醇亚基。已经从林业物种中分离出编码RAC2(SEQ ID NOS:28-35)和gp 91 NADPH叶绿醇亚基(SEQ ID NOS:192)相关多肽的多核苷酸。SEQ ID NOS:28和30-45中给出的多核苷酸编码的RAC-2相关多肽之相应预测氨基酸序列见SEQ ID NOS:73和74-79。该gp 91 NADPH叶绿醇亚基相关多肽的相应预测氨基酸序列见SEQ ID NO:196。
arabidopsis中损伤模拟细胞死亡(lsd1)突变体的发现阐述了超氧化物在植物细胞死亡中的作用。在该突变体中,超氧化物足以诱导和传播细胞死亡且是必须的。认为Lsd1在野生型植物中充当超氧化物依赖信号的监视器并作为植物细胞死亡途径的负调节剂。已经从林业物种中分离出编码lsd1-相关多肽的多核苷酸(SEQ ID NOS:13和14)。将该多核苷酸编码的lsd1-相关多肽确定为SEQ ID NOS:58和59。
将ATL2确定为Arabidopsis cDNA,当在酵母中高表达时其是有毒性的。SEQ ID NOS:1-5给出从林业物种中分离的5个ATL2变体的核苷酸序列,并且相应的预测氨基酸序列见SEQ ID NOS:46-50。
自玉米突变体识别的其它的基因,lls1也用在叶子中以发育方式限制细胞死亡的扩散。已经从林业物种中分离出编码致死叶斑蛋白lls1相关多肽(SEQ ID NOS:11-12)的多核苷酸。将该多核苷酸编码的多肽确定为SEQ ID NOS:56-57。
已发现其它的Arabidopsis植物蛋白质(oxy5)为蛋白质的膜联蛋白家族成员并保护细胞免受氧应激。Oxy5也表现为保护哺乳动物细胞免于肿瘤坏死因子-诱导的细胞死亡。在植物中在多个PCD的例子中氧应激的参与提示oxy5起保护作用。该膜联蛋白序列表现与oxy5具有很好的同源性,因此推测其具有相同或相似的功能。在SEQ IDNOS:17-21中给出从林业物种中分离的膜联蛋白类蛋白质的核苷酸序列,相应的预测氨基酸序列见SEQ ID NOS:62-66。
病原体的超敏反应(HR)是最多研究的植物中PCD的实例。在大多数疾病抵抗(RO基因介导的反应中发现HR。病原体无毒基因产物与受体的结合产生该HR。其启动包括离子溢出、激酶/磷酸酶作用和氧化性爆发在内的级联事件,导致定位细胞死亡和诱导系统获得性抵抗(SAR),其中植物的其它部分产生对病原体的获得性抵抗。已经识别大量的植物疾病受体,包括跨越细胞膜和含有细胞外及细胞质结构域的多肽,以及不含有细胞外结构域的严格细胞质的多肽。
在参与HR的细胞质多肽受体中,主要关注三个家族。第一是RPS2-样家族,其中该多肽含有氨基端亮氨酸折叠区、核苷酸结合位点、内疏水区和羧基端富亮氨酸重复。第二个是RPP5-样家族,其中该多肽含有氨基端Toll类结构域、核苷酸结合位点、内疏水区和羧基端富亮氨酸重复区。在SEQ ID NOS:126-140中给出从林业物种中分离的RPP5类蛋白质的核苷酸序列,相应预测的氨基酸序列见SEQ IDNOS:177-191。
参与HR的细胞质受体的第三个家族是PTO-样家族,其中该多肽含有丝氨酸-苏氨酸激酶结构域。尽管随着几个PTO-相互作用蛋白质基因的识别,已发现在PTO-样家族中有蛋白质-蛋白质相互作用和激酶反应的参与,但是该HR细胞死亡信号转导的确切机制不明。
该最初的无毒性/受体相互作用之后,发生SAR的进展,其中有NPR1基因参与。NPR1基因的突变增加植物对病原体感染的易感性并防止HR PCD和SAR的进展。在转基因植物中R基因的表达使得HR PCD发展和抵抗特异病原体。另外,在不存在病原体的情况下PTO-样家族成员如Fen的表达能导致PCD。在SEQ ID NOS:193-195中给出编码从林业物种中分离的NPR1类蛋白质的核苷酸序列并且相应预测的氨基酸序列见SEQ ID NOS:197-199。在SEQ ID NOS:27中给出编码从林业物种中分离的Fen类蛋白质的核苷酸序列并且相应预测的氨基酸序列见SEQ ID NOS:72。在其它植物PCD实例中这些基因的作用不明。令人感兴趣的是发现该R基因家族和NPRI与参与动物发育和防御的几个蛋白质具有相似性。将发育过程与疾病抵抗相连的共同途径的发现提示HR-相关基因可能在其他植物PCD和发育途径中起作用。
第一方面,本发明提供在所附序列表中确定为SEQ ID NOS:1-45、90-140、192-195、200、202和206的分离的多核苷酸序列;该序列的变体;含有SEQ ID NOS:1-45、90-140、192-195、200、202和206所示序列的延伸序列及其变体;SEQ ID NOS:1-45、90-140、192-195、200、202和206所示序列的延伸序列及其变体的相应探针和引物;含有任一确定为SEQ ID NOS:1-45、90-140、192-195、200、202和206(x-链节)的多核苷酸的至少指定数目邻近残基的多核苷酸;和含有SEQ ID NOS:1-45、90-140、192-195、200、202和206中所示序列片段的延伸序列;所有的这些作为“本发明的多核苷酸”在此供作参考。本发明还提供在所附序列表中确定为SEQ ID NOS:46-89、141-191、196-199、201和205的分离的多核苷酸序列;该序列的多肽变体;和含有该分离的多肽序列和该序列变体的多肽。
确定为SEQ ID NOS:1-45、90-140、192-195、200、202和206的多核苷酸,来源于林业物种资源,即来自巨桉(Eucalyptusgrandis)和辐射松(Pinus radiata)。本发明的一些多核苷酸是“部分”序列,因为它们不代表编码全长多肽的全长基因。应用引物和/或探针及共知的杂交和/或PCR技术,通过分析和对多种DNA文库进行测序可延伸这些不完全序列。可延伸部分序列直至编码多肽的开放阅读框架、全长多核苷酸和/或能表达多肽的基因或该基因组的其它有用部分得以确定。当该延伸的多核苷酸含有SEQ ID NOS:1-45、90-140、192-195、200、202和206或其变体中一个序列的识别序列或其变体,或识别的邻近部分(x-链节)时,将这些延伸序列,包括全长多核苷酸和基因描述为“对应于”确定为SEQ ID NOS:1-45、90-140、192-195、200、202和206或其变体的一个序列,或SEQ ID NOS:1-45、90-140、192-195、200、202和206中一个序列的部分。相似地,应用确定为SEQ ID NOS:1-45、90-140、192-195、200、202和206的cDNA序列,可用常规方法确定和获得与本发明的多核苷酸对应的RNA序列、反向序列、互补序列、反义序列等。
确定为SEQ ID NOS:1-45、90-140、192-195、200、202和206的多核苷酸可含有编码多肽的开放阅读框架(“ORFs”)或部分开放阅读框架。而且,在与SEQ ID NOS:1-45、90-140、192-195、200、202和206所示序列对应的延伸或全长序列中可识别出编码多肽的开放阅读框架。应用本领域共知的技术可识别开放阅读框架。这些技术包括,例如,分析已知起始和终止密码子的定位,基于密码子频率最可能阅读框架的确定等。例如,在国际互联网http://www.ncbi.nlm.nih.gov/gorf/gorf.html.上可获得ORF分析的合适工具和软件。可确定本发明多核苷酸的开放阅读框架和部分开放阅读框架。一旦开放部分阅读框架确定,在该部分开放阅读框架区域应用本领域共知的技术可延伸此多核苷酸直至全长开放阅读框架得以确定。这样应用本发明的多核苷酸可确定开放阅读框架。
一旦本发明多核苷酸的开放阅读框架确定,即可分离和/或合成此开放阅读框架。然后可构建本领域共知的含有该开放阅读框架和合适启动子、起始密码子、终止子等的可表达遗传构建体。可将此遗传构建体导入宿主细胞中以表达该开放阅读框架编码的多肽。适宜的宿主细胞可包括多种原核和真核细胞,包括植物细胞、哺乳动物细胞、细菌细胞、藻类细胞等等。
可表达本发明多核苷酸编码的多肽并用于多种分析以测定它们的生物学活性。此多肽可用于生成抗体,分离对应的相互作用的蛋白质或其它化合物,并定量测定相互作用蛋白质的水平或其它化合物。
本发明也涉及调节林业物种的该多核苷酸和/或多肽内容和成分的方法,此方法包括向该生物基因组稳定插入含有一个或多个本发明多核苷酸的遗传构建体。在实施方案中,该靶生物是林业物种,优选木本植物,更优选松属或桉树属木本植物,并最优选辐射松或巨桉。在相关方面,提供生产具有改变基因型和表型林业植物的方法,此方法包括用本发明的遗传构建体转化植物细胞以提供转基因细胞,并在有益于植物生长再生和成熟的条件下培养该转基因细胞。本发明还涉及和包括这种林业植物:与野生型生物相比,作为调节本发明的多核苷酸或多肽的水平或含量的结果,该生物具有改变了的基因型或表现型;也涉及和包括这些生物的各部分(种子,等)以及这些生物的后代。
本发明分离的多核苷酸也具有基因组绘图、物理绘图和基因定位克隆用途。另外,可应用SEQ ID NOS:1-45、90-140、192-195、200、202和206确定的多核苷酸及其变体设计寡核苷酸探针和引物。寡核苷酸探针和引物具有基本上与该多核苷酸某些部分的兴趣多核苷酸互补的序列。应用本领域共知的技术,如狭线DNA杂交技术,本发明的多核苷酸设计的寡核苷酸探针可用于检测任何生物内基因的存在和检测基因的表达模式,该生物细胞内具有足够相似DNA和RNA序列。应用本发明多核苷酸设计的寡核苷酸引物可用于PCR扩增。还可将应用本发明多核苷酸设计的寡核苷酸探针和引物与多种微阵列技术,包括Synteni所用的微阵列技术(Palo Alto,Califoria)结合应用。
本发明的多核苷酸还可用于标记或确定生物或由此得到的再生材料。例如通过向生物中稳定导入非破坏性无功能性异源多核苷酸标记物完成这些标记,该多核苷酸含有一个本发明的多核苷酸。
在植物中,本发明多肽和编码该多肽的多核苷酸在PCD和多种发育途径中具有活性。通过DNA和多肽相似性研究推导确定该多核苷酸。在附加序列表中,SEQ ID NOS:1-28、30-45、90-140、192-195、200和204是分别编码SEQ ID NOS:46-73、74-89、141-191、196-199、201和205中所列多肽的多核苷酸序列。本发明多核苷酸和多肽证实与下列多肽具有相似性,已知这些多肽参与PCD和/或植物发育过程。
表1
多肽名称 | 多核苷酸SEQID NO. | 多肽SEQ IDNO. |
ATL2 | 1-5 | 46-50 |
DADI(抗细胞死亡防护剂) | 6-9 | 51-54 |
脱氧核糖核酸酶 | 10 | 55 |
lls(致命叶斑) | 11、12 | 56、57 |
lsd1(损害刺激死亡) | 13、14 | 58、59 |
Nucellin类天冬氨酸蛋白酶 | 15、16 | 60、61 |
膜联蛋白 | 17-21 | 62-66 |
抑制素 | 22-26 | 67-71 |
Fen类蛋白质 | 27 | 72 |
RAC2 | 28-35 | 73、74-79 |
视网膜母细胞瘤相关蛋白 | 36、37 | 80、81 |
SINA(Seven in absentia) | 38-40、200 | 82-84、201 |
TFIID(转录起始因子) | 41、42 | 85、86 |
TEGT(睾丸增加的基因转录物) | 43、44 | 87、88 |
木纤维核糖核酸酶 | 45 | 89 |
Pur-alpha | 90、91 | 141、142 |
半胱氨酸蛋白酶 | 92-125 | 143-176 |
RPP5类蛋白质 | 126-140 | 177-191 |
gp 91 NADPH叶绿醇亚基 | 192 | 196 |
NPR类蛋白质 | 193-195 | 197-199 |
BAG-1 | 204 | 205 |
在一实施方案中,本发明分离的多核苷酸含有从下组中选择的序列,包括:(a)SEQ ID NOS:1-45、90-140、192-195、200、202和206中列举的序列;(b)SEQ ID NOS:1-45、90-140、192-195、200、202和206中所列序列的互补序列;(c)SEQ ID NOS:1-45、90-140、192-195、200、202和206中所列序列的反向互补序列;(d)SEQ ID NOS:1-45、90-140、192-195、200、202和206中所列序列的反向序列;和(e)具有与(a)-(d)之序列或其某些区域至少如此处定义50%、75%、90%或98%同一性的序列。
另外,提供本发明多核苷酸编码的分离多肽。在一实施方案中,这些多肽含有SEQ ID NOS:46-89、141-191、196-199、201和205及其变体中所列的氨基酸序列,和本发明多核苷酸包括含有SEQ IDNOS:1-45、90-140、192-195、200和204序列表达的多肽。
另一方面,本发明提供含有本发明多核苷酸的遗传构建体,该遗传构建体含有的多核苷酸是单独的、或与一个或多个另外的本发明多核苷酸结合、或与一个或多个已知的多核苷酸结合,并提供含有这些构建体的细胞和靶生物。
相关方面,本发明提供含有5’-3’方向基因启动子序列、编码本发明多核苷酸编码多肽至少一个功能区域的开放阅读框架和基因终止序列。可将开放阅读框架按有义或反义方向放置。还涉及含有基因启动子序列、本发明多核苷酸和基因终止序列的遗传构建体,及含有基因启动子序列、本发明多核苷酸非翻译区或与非翻译区互补的核苷酸序列和基因终止序列的遗传构建体。该遗传构建体还可包括用于识别转化细胞的标记物。
该基因启动子和终止序列优选在宿主植物中具有功能的,最优选宿主植物天然的序列。应用本领域内通用的启动子和终止序列,如花椰菜花叶病毒(CMV)启动子,有或无增强子例如Kozak序列或Omega增强子和根瘤土壤杆菌胭脂碱合酶增强子。为靶表达一个或多个预期的组织可采用组织-特异性启动子。
在其它方面,提供制备林业植物的方法,与天然生物相比该植物具有本发明多核苷酸或多肽的改变含量。提供用本发明遗传构建体转化林业植物以提供转基因细胞和在有助于再生和成熟植物生长的条件下培养该转基因细胞的方法。提供含有本发明遗传构建体的细胞,并提供含有这些转基因细胞的组织和林业植物,及果实、种子和其它产物、衍化物或这些林业植物的子代植物。
在本发明的其它方面,提供调节PCD和调节植物多种发育途径的方法,这些方法包括向林业植物基因组中稳定导入本发明遗传构建体。更具体地,提供调节发育途径包括木材发育、衰老和生殖发育的方法,和在植物中调节应激反应的方法。优选的林业植物包括木本植物,优选选自桉树、松树、金合欢、白杨、胶皮枫香树、柚木和桃花心木类,更优选选自松树和桉树类,最优选选自巨桉和辐射松。
通过参考下面更详细的描述,本发明的上述和另外特点及获得它们的方式将更加明了,并且本发明将得到更好的理解。
示图简述
图1示用辐射松DAD1基因(SEQ ID NO:8)反义序列转化烟草植物的Southern印记分析。
图2示用辐射松DAD1基因(SEQ ID NO:8)反义序列转化烟草植物的Northern印记分析。
图3图示在转化烟草植物中松树单一序列标记物的检测。泳道A和B表示来自SEQ ID NO:202的探针与烟草植物基因组DNA的杂交(情况),该烟草植物缺少松树单一序列标记物(空载体转化对照载体或野生型植物)。泳道C-E表示该探针与烟草植物基因组DNA的杂交(情况),该植物含有一到三个该松树单一序列标记物拷贝。
图4图示在转化烟草植物中桉树单一序列标记物的检测。泳道A和B表示来自SEQ ID NO:203的探针与烟草植物基因组DNA的杂交(情况),该烟草植物缺少松树单一序列标记物(空载体转化对照载体或野生型植物)。泳道C-E表示该探针与烟草植物基因组DNA的杂交(情况),该植物含有一到二个该桉树单一序列标记物拷贝。
优选实施方案描述
应用本发明的材料和方法,通过修饰该靶生物的多核苷酸和/或多肽内容,例如,通过向该林业植物基因组中导入本发明多核苷酸的有义或反义拷贝,该多核苷酸编码参与PCD和/或特异发育途径的多肽,可调节林业植物PCD和/或特异性发育途径。而且,可在林业植物中控制本发明多核苷酸的拷贝数目和结合,以改变合成多肽的相对数量,从而产生具有改变成分和/或改变发育代谢的生物物质。
根据一实施方案,本发明提供编码或部分编码多肽的分离多核苷酸,该多肽参与林业植物中PCD和/或特异发育途径。从桉树和松树类中分离本发明的多核苷酸,或者可应用传统的合成技术合成。具体地,本发明的分离多核苷酸包括含有下列序列的多核苷酸,该组序列有:确定为SEQ ID NOS:1-45、90-140、192-195、200、204和206的序列;确定为SEQ ID NOS:1-45、90-140、192-195、200、204和206的序列的互补序列;确定为SEQ ID NOS:1-45、90-140、192-195、200、204和206的序列的反向序列;确定为SEQ ID NOS:1-45、90-140、192-195、200、204和206的序列的反向互补序列;任何上述多核苷酸的至少特定数目的邻接残基(x-链节);任何上述多核苷酸的相应反义序列;和任何上述多核苷酸的变体,该术语如此说明书所述。
在SEQ ID NOS:1-45、90-140、192-195、200和204中列举的分离的多核苷酸分别编码或部分地编码在SEQ ID NOS:46-89、141-191、196-199、201和205中列举的参与PCD和/或发育过程的多肽,如表1所示。应用本发明的方法和材料,通过导入多种本发明的多核苷酸,包括这些多核苷酸的非翻译区和这些多核苷酸的反义拷贝,可升高和降低靶生物,如林业物种的多核苷酸和/或多肽含量,从而调节在生物中或该生物组织中PCD,或调节生物中或组织中发育途径。
在另外的实施方案中,本发明提供SEQ ID NOS:1-45、90-140、192-195、200和204的DNA序列编码的分离多肽。依据整理此申请书之时可获得的信息,分别在SEQ ID NOS:46-73、74-89、141-191、196-199、201和205中提供SEQ ID NOS:1-45、90-140、192-195、200和204中所示多核苷酸对应的预测氨基酸序列。
该术语“多核苷酸”,如此处所用,意为脱氧核糖核苷酸或核糖核苷酸碱基的单或双链聚合物并包括DNA和相应的RNA分子,包括HnRNA和mRNA分子,有义和反义链,并包括cDNA、基因组DNA和重组DNA,及整个或部分合成的多核苷酸。HnRNA分子含有内含子并以大致一对一的方式与DNA分子相一致。mRNA分子与HnRNA和切去内含子的DNA分子对应。多核苷酸可含有完整基因,或其任何片段。基因是编码功能蛋白质或RNA分子的DNA序列。可操作性反义多核苷酸可含有该对应多核苷酸的片段,所以“多核苷酸”的定义包括所有这样的可操作性反义片段。反义多核苷酸和涉及反义多核苷酸的技术是本领域共知的,并且在例如,Robinson-Benion等人,“反义技术”,酶学方法(Methods in Enzymol).254(23):363-375(1995);Kawasaki,等人,人工器官(Artific Organs)20(8):836-848(1996)中有所描述。本发明的多核苷酸还包括与已发现序列不同的多核苷酸序列,但是作为遗传密码简并的结果,其编码的多肽与此处公开的DNA序列编码的序列相同。
在此应用的术语“互补”、“反向互补”和“反向序列”的定义通过下面的实施例得以很好的阐释。就序列5’AGGACC 3’而言,该互补、反向互补和反向序列如下:
互补 3’TCCTGG 5’
反向互补 3’GGTCCT 5’
反向序列 5’CCAGGA 3’。
在适宜严格的条件下,应用所有或部分cDNA序列作为探针检测适宜的文库,通过标准的DNA/DNA杂交技术可完成基因组DNA和异源DNA的确定。或者,应用依据已知基因组DNA、cDNA和蛋白质序列设计的寡核苷酸引物,PCR技术,可用于扩增和确定基因组和cDNA序列。通过传统的合成方法可制备该确定序列和变体对应的合成性DNA。将此处描述的多核苷酸分离和纯化,那些术语是本领域中普遍应用的。
其它方面,本发明提供上述多核苷酸编码或部分编码的分离多肽。在此应用,术语“多肽”包括任何长度的氨基酸链,包括全长蛋白质,其中氨基酸残基通过共价肽键连接。在此应用的术语“多核苷酸编码的多肽”包括多核苷酸编码的多肽,该多核苷酸包括在此提供的分离的DNA序列或变体。在某些实施方案中,本发明多肽包括的氨基酸序列选自下组序列:SEQ ID NOS:46-89、141-191、196-199、201和205及这些序列的变体。
通过向表达载体插入编码该多肽的DNA序列和在适宜宿主中表达该多肽,可重组制备本发明的多肽。可采用本领域普通技术人员所知的任何的多种载体。在任何适宜的宿主细胞中可完成表达,该宿主细胞已为含有编码重组多肽DNA分子的表达载体转化或转染。适宜的载体包括原核生物、酵母和高级真核细胞。优选采用的宿主细胞是大肠杆菌、昆虫、酵母或哺乳动物细胞系例如COS或CHO。以此方式表达的该DNA序列可编码自然发生多肽、自然发生多肽片段或其其它的突变体。
在相关方面,提供含有至少一个多肽功能区的多肽,该多肽含有从下组中选择的氨基酸序列,该组由SEQ ID NOS:46-89、141-191、196-199、201和205及其变体所提供的序列组成。在此应用,多肽的“功能区”是指含有影响该多肽功能的必需活性位点的区域,例如该区域分子能与一个或多个反应物结合。该活性位点由在一个或多个多肽链上呈现的分离区域组成,并通常将表现高结合亲和力。
可通过化学或酶酶切该多肽首先制备该多肽的片段,或通过分析编码该多肽的多核苷酸的突变及之后表达该获得的突变多肽,来识别多肽的功能区。然后,应用例如下面提供的代表性分析检测该多肽片段或突变多肽,以确定何区域保留生物学活性。
含有活性位点的功能区域可由在一个或多个多肽链上呈现的和通常表现高底物特异性的分离区域组成。在此应用的术语“多核苷酸编码的多肽”包括多核苷酸编码的多肽,该多核苷酸含有本发明部分分离的多核苷酸。
通过合成或重组方法也可生成本发明多肽的部分和其它变体。合成多肽不超过100个氨基酸,一般不超过大约50氨基酸,应用本领域普通技术人员共知的技术可生成之。例如,应用任何的商业提供的固-相技术,例如Merrifield固-相合成方法,其中将氨基酸连续地加入增长的氨基酸链上,可合成此多肽。自动合成多肽的仪器由提供者例如Perkin Elmer/Applied Biosystems,Inc(Foster City,California)商业提供,根据制造商的说明书可进行操作。应用标准诱变技术例如,寡核苷酸定向位点特异性诱变(Kunkel,T.,美国国家科学院院报(Proc.Natl.Acad.Sci.USA)82:488-492,1985)可制备天然多肽的变体。应用标准技术还可去除DNA序列片段以制备截短多肽。
总而言之,以分离的、大体上纯化的方式制备此处所示的多肽。优选,该多肽至少大约80%纯化;更优选至少大约90%纯化,最优选至少大约99%纯化。在下面详述的某些优选的实施方案中,将该分离的多肽掺入药用成分中或疫苗中用于治疗皮肤疾病。
在此应用的术语“变体”包括与该特异识别序列不同的核苷酸或氨基酸序列,其中缺失、替换或增加一个或多个核苷酸或氨基酸残基。变体可以是自然发生等位基因的变体,或非自然发生变体。变体序列(多核苷酸或多肽)优选与本发明的序列相比表现至少50%;更优选至少75%;最优选至少90%的同一性。测定该同一性百分比,方法是:通过如下排列比较该两个序列、测定匹配区域的同一性残基的数目、将该数目除以本发明(查询)序列的残基总数并将该结果乘以100。
应用公共提供的计算机程序,可进行多核苷酸和多肽序列配对,并测定在某区域与其它多核苷酸的同一性核苷酸百分比。匹配和确定多核苷酸序列相似性的两个示范性程序是BLASTN和FASTA程序。应用BLASTX还可分析多核苷酸,其将核苷酸查询序列(双链)的六-框架概念翻译产物与蛋白质序列数据库进行比较。应用BLASTP程序可检测多肽序列的相似性。在NCBI无名FTP服务器(ftp://ncbi.nlm.nih.gov)/blast/executables/下提供BLASTN、BLASTX和BLASTP程序。该BLASTN程序版本2.0.4[Feb-24-1998]和版本2.0.6[Sep-16-1998]优选用于测定关于本发明的多核苷酸变体,其设置文献中描述的和随程序分配的缺省参数。该BLASTP程序,设置文献中描述的和随程序分配的缺省参数,优选用于测定关于本发明的多核苷酸变体。在NCBI网站URL http://www.ncbi.nlm.nih.gov/BLAST/newblast.html和在ALTSCHUL、STEPHEN F.等人“裂隙BLAST和PSI-BLAST:新一代的蛋白质数据库搜索程序”核酸研究(Nucleic Acids Res).25:3389-3402,1997中描述BLAST家族程序,包括BLASTN、BLASTP和BLASTX的应用。
在Internet的ftp站点ftp://ftp.virginia.edu/pub/fasta/可以获得计算机程序FASTA。2.0u4版本(1996年2月),按照文件中描述的及随着程序面扩散的有默认的参数设置,可用来测定依据本发明的变体。对FASTA程序应用的描述见于Pearson和Lipman,美国科学院院报85:2444-2448,1998;及Pearson WR“应用FASTP和FASTA的快速和敏感序列比较”,酶学方法(Methods in Enzymol.)183:63-98,1990。
应用对E值和同一性百分比起作用的BLASTN测定多核苷酸序列的匹配和相似性,下列运行参数是优选的:Unix运行命令:blastall-Pblastn-d emblbd-e 10-Go-Eo-r 1-v 30-b 30-i queryseq-o结果;参数是:-P程序名[列];-d数据库[列];-e期望值(E)[实数];-G打开一个间隔的贡献(零引起无效行为)[整数];-E延伸一个间隔的贡献(零引起无效行为)[整数];-r一个核苷酸配对的获得值(仅用于BLASTN)[整数];-v单线描述的数字(V)[整数];-b显示的匹配数目(B)[整数];-i询问文件[文件输入];及-0BLAST报告输出文件[文件输出]选择。应用对E值和同一性百分比起作用的BLASTP测定多肽序列的匹配和相似性,下述运行参数是优选的:blastall-p blastp-d swissprotdb-e 10-G O-E O-v 30-b 30-i queryseq-0结果;参数是:-p程序名[列];-d数据库[列];-e期望值(E)[实数];-G打开一个间隔的贡献(零引起无效行为)[整数];-E延伸一个间隔的贡献(零引起无效行为)[整数];-v单线描述的数字(v)[整数];-b显示的匹配数目(b)[整数];-I询问文件[文件输入];-o BLAST报告输出文件[文件输出]选择。通过BLASTN,FASTA,BLASTP或相似程序产生的询问序列对一或多个数据库序列“点击”,进行匹配并识别序列的相同部分。将命中序列按照相似性的程度及序列重叠长度进行排列。一个数据库序列的命中序列通常代表只和询问序列的一部分序列长度相重叠。
BLASTN,FASTA和BLASTP程序也产生匹配的“期望”值。期望值(E)是指当搜索特定大小的数据库时随机察看特定数目的连续序列可以“期望”的命中物的数目。该期望值用作重要的阈值,用以测定命中数据库例如优选的EMBL数据库是否表示真正的相似性。例如,赋予多核苷酸命中者0.1的E值可解释成:在EMBL数据库大小的数据库中,仅通过随机可预期观察到具有相似值的序列的该匹配部分0.1的配对。按照这条标准,多核苷酸序列匹配和配对部分相同的可能性则为90%。对于匹配和配对部分E值为0.01或更小的序列,在EMBL数据库中应用BLASTN或FASTA程序随机发现配对的可能性是1%或更小。
根据一个实施方案,关于本发明的每一个多核苷酸和多肽,“变体”多核苷酸和多肽,优选包括这种序列,其具有本发明多核苷酸或多肽相同或更少数目的核酸或氨基酸,并且将其与本发明的多核苷酸或多肽对比时产生的为E值0.01或更小。即,变体多核苷酸或多肽是指与本发明多核苷酸或多肽序列相同可能性至少为99%的任何序列,在用BLASTN,FASTA或BLASTP程序按上述设定的参数测定时具有0.01或更小的E值。根据优选的实施方案,变体多核苷酸是指具有和本发明多核苷酸相同或较少数目核酸的序列,与本发明的多核苷酸相同的可能性至少为99%,使用如上述设定参数的BLASTN或PASTA程序测定的E值为0.01或更小。类似地,根据优选实施方案,变体多肽是指具有与本发明多肽相同或较少数目氨基酸的序列,与本发明多肽的相同可能性至少为99%,用BLASTP程序按上述设定参数测定的E值为0.01或更小。
或者,本发明的变体多核苷酸或多肽包括的序列显示出和本发明的多核苷酸或多肽至少50%的同一性;更优选至少75%;更进一步优选地至少90%;最优选地至少98%的同一性,测定方法如下所述。通过使用BLASTN、FASTA或BLASTP程序之一进行序列配对,该程序按上述设定运行参数,并确定该配对部分相同核苷酸或氨基酸的数目;将相同核苷酸或氨基酸的数目除以本发明多核苷酸或多肽之核苷酸或氨基酸的总数目;然后再乘以100以确定该同一性百分比。例如,通过在用BLASTN程序应用上述参数产生的匹配中,具有220个核酸的本发明的多核苷酸与EMBL数据库中具有520个核苷酸的多核苷酸有连续23个核苷酸相匹配。命中的23个核苷酸包括21个相同核苷酸,一个间隔和一个不同核苷酸。本发明多核苷酸与EMBL文库中命中者的相似性百分比就是21/220×100,或9.5%。因此,EMBL数据库中的该多核苷酸序列不是本发明多核苷酸的变体。
或者,在严格条件下,本发明的变体多核苷酸与下列多核苷酸杂交:SEQ IDNOS:SEQ ID NOS:1-45、90-140、192-195、200、204和206所列的多核苷酸序列,或那些序列的互补体,反向序列,或反向互补体。正如此处所用的,“严格条件”是指在6×SSC、0.2%SDS的溶液中预清洗;在65℃、6×SSC、0.2%SDS条件下杂交过夜;接着是两次各30分钟的清洗,条件为1×SSC、0.1%SDS、65℃和两次清洗,0.2×SSC、0.1%×SDS、65℃各30分钟。
本发明也包括与发现的序列不同、但是由于遗传密码的简并性,其编码与本发明多核苷酸编码多肽有相似酶活性的多肽的多核苷酸。这样,包括由于保守替换而不同于SEQ ID NOS:1-45、90-140、192-195、200、204和206所述多核苷酸序列,或其互补体、反向序列、或反向互补体的序列的多核苷酸,考虑并位于本发明范围内。另外,本发明亦涉及和包括含有下列序列的多核苷酸,该序列为由于缺失和/或插入而与SEQ ID NOS:1-45、90-140、192-195、200、204和206所述多核苷酸序列,或互补体,反向互补,或反向序列有区别的序列,其缺失和/或插入的总体小于总序列长度的10%。相似地,本发明涉及和包括含有下列序列的多核苷酸,该序列为由于氨基酸替换、插入和/或缺失而与SEP ID NOS:46-89、141-191、196-199、201和205所述的多肽序列有差异的序列,但氨基酸替换、插入和/或缺失总长度小于序列总长度的10%,前提是该变体多肽在PCD或植物发育途径中有活性。
可以从不同的文库中分离或用本领域内共知的技术合成本发明的多核苷酸。例如,应用自动寡核苷酸合成仪(例如,Beckman Dligo1000M DNA合成仪)可以合成多核苷酸,以获得50或更多核酸的多核苷酸片段。而后就可以用分子生物学领域中广为人知的标准DNA操作技术将许多这样的多核苷酸片段连接起来。一个传统的和示范性的多核苷酸合成技术包括合成比如含有80个核苷酸的单链多核苷酸片段,将此片段与一个合成的互补性的85个核苷酸的片段杂交,以产生5个核苷酸的突出端。可以用相似的方式合成下一个片段,在相对链上具有5个核苷酸的突出端。当该两部分杂交时,此“粘性”末端保证了正确连接。用这种方式,可以完全在体外合成本发明的完整多核苷酸。
将确定为SEQ ID NOS:1-53和78-164的一些多核苷酸称为“部分”序列,因为它们并不代表编码自然存在多肽的基因的全长编码部分。通过例如,用基于本发明多核苷酸的杂交探针筛选DNA表达文库,或者用基于本发明多核苷酸的引物进行PCR扩增,可将此处公开的部分多核苷酸序列用来获得不同物种或生物的相应全长基因。以这种方式,运用本领域内共知的方法,可以将相应mRNA的本发明多核苷酸向上游和下游延伸,还可以鉴定对应的基因组DNA,包括完整基因启动子和增强子区域。这样,本发明包括编码功能多肽包括全长基因的分离多核苷酸,其包括SEQ ID NOS:1-53和78-164中确定的序列,或其中一个特定序列的变体。这些延伸的多核苷酸的长度从约50个到约4000个核苷酸或碱基对不等,优选长度小于约4000个核苷酸或碱基对,更优选长度小于约3000核苷酸或碱基对,更进一步优选长度小于2000核苷酸或碱基对。在某些条件下,本发明的延伸多核苷酸的长度小于约1800核苷酸或碱基对,优选地小于约1600核苷酸或碱基对,更加优选地小于约1400核苷酸或碱基对,更进一步优选小于约1200核苷酸或碱基对,最优选地小于约1000核苷酸或碱基对。
本发明的多核苷酸也包括下述多核苷酸,该多核苷酸含有鉴定为SEQ ID NOS:1-45、90-140、192-195、200、204和206的任何多核苷酸、这些序列的互补体、反向序列和反向互补体及其变体中至少特定数目的相邻残基(X-链节)。类似地,含有鉴定为SEQ ID NOS:46-89、141-191、196-199、201和205任一种多肽及其变体中至少特定数目的相邻残基(X-链节)的多肽,也包括在本发明的多肽之内。此处所用的术语“X-链节”,关于“X”的特定值,是指含有任一鉴定为SEQ ID NOS:1-45、90-140、192-195、200、204和206的多核苷酸,或鉴定为SEQ ID NOS:46-89、141-191、196-199、201和205的多肽当中至少特定数目(“X”)相邻残基的序列。根据优选的实施方案,X的值优选至少为20;更优选地,至少40;进一步优选地,至少60;最优选至少80。这样,本发明多核苷酸或多肽包括鉴定为SEQ ID NOS:1-201和204-206的多核苷酸或多肽或其变体的20-链节、40-链节、60-链节、80-链节、100-链节、120-链节、150-链节、180-链节、220-链节、250-链节、或300-链节、400-链节、500-链节或600-链节。
互补于和/或对应于SEQ ID NOS:1-45、90-140、192-195、200、204和206的以及那些序列变体的多肽苷酸探针和引物,也包括在本发明之中。这种寡核苷酸探针和引物主要与该兴趣多核苷酸互补。此处所用的术语“寡核苷酸”指的是多核苷酸序列的相对较短的片段,通常含有6到60个核苷酸,并包括杂交分析中应用的探针以及通过聚合酶链反应扩增DNA当中应用的引物。
当SEQ ID NOS:1-45、90-140、192-195、200、204和206中所示的一个序列或某一序列的变体中含有该寡核苷酸探针或引物或其互补体时,将该寡核苷酸探针和引物描述成“对应于”本发明的多核苷酸,包括SEQ ID NOS:1-45、90-140、192-195、200、204和206中所示的序列或或变体。
具有适宜核苷酸插入和/或缺失的一条链的核苷酸和另一链的核苷酸最优匹配和比较时,至少80%配对,优选至少90%到95%,更优选至少98%到100%配对,就将两条单链序列说成是基本上互补。或者,当第一DNA链在严格的杂交条件下选择性地和第二DNA链杂交时基本互补性存在。测定互补性的严格杂交条件包括:盐浓度小于约1M,更经常地要小于约500mM,优选地小于200mM。杂交温度可以低至5℃,但通常要高于约22℃,更优选高于约30℃,最优选高于约37℃。较长的DNA片段可能需要更高的杂交温度以进行单一性杂交。由于杂交的严格性可以受其他因素,诸如探针的组成、有机溶剂的存在和碱基错配的影响,所以各种参数的组合要比任一单因素的绝对数值更重要。来自植物的或含有植物材料的样品或产品的DNA,可以是基因组DNA或通过样品中RNA制备cDNA再衍生出的DNA。
除DNA-DNA杂交之外,也可以进行DNA-RNA或RNA-RNA杂交分析。在第一种情况下,可取代基因组DNA或衍自样品mRNA的cDNA而检测来自表达基因的mRNA。在第二种情况,可利用RNA探针。另外,也可以应用与靶序列特异性杂交的DNA的人工类似物。
在某些实施方案中,该寡核苷酸探针和/或引物包括至少约6个与本发明的多核苷酸序列互补的相邻残基,更优选地至少约10个相邻残基,最优选地至少约20个相邻残基。本发明的探针和引物的长度可以是约8到100个碱基对,或者,优选地长度约10到50个碱基对,或者更加优选地长度从约15到40个碱基对。用本领域共知的方法,容易筛选探针,这些方法涉及DNA-DNA杂交的严格性,退火和解链温度,环形成的可能性及领域内共知的其他因素。可以从Internet上,例如,在URL http://www.horizonpress.com/pcr/获得适合设计探针的尤其是适合设计PCR引物的工具和软件。设计PCR引物的优选技术在Dieffenbach CW和Dyksler GS的著作中也有公开,见:PCR引物:实验手册(PCR Primeia laboratory manual),CSHL出版社:冷泉港,纽约,1995。
以试剂盒的形式可提供许多与本发明多核苷酸对应的寡核苷酸探针或引物。这些试剂盒通常包括多种DNA或寡核苷酸探针,每一种探针特异性地针对一种多核苷酸序列。本发明的试剂盒可以含有一种或多种与本发明多核苷酸对应的探针或引物,本发明的多核苷酸包括SEQ ID NOS:1-45、90-140、192-195、200、204和206中所确定的多核苷酸序列。
在一个有益于高通量分析实施方案中,本发明的寡核苷酸探针试剂盒含有以阵列形式排布的多种探针,其中将每一种探针固定在固体底物表面的预先规定的、空间上可编址的位置上。本发明中可以有效应用的阵列形式公开于,例如,美国专利号5,412,087和5,545,451;和PCT公开号WO 95/00530,其分开的内容此处引用作为参考。
引物,优选地以阵列形式排列,应用本领域共知的高通量筛选技术,可用来筛选生物中或含有遗传物质的样品或产品中的差异。对于以下应用,在高通量筛选系统中应用探针的重要性是明显的,例如植物育种和质量控制操作,其中需要鉴定大数量的种子批号和植物幼苗,检测样品或产品中不需要的植物材料,为了检疫的目的鉴定植物或含有植物材料的样品或产品,等等,或者鉴定植物或含有植物材料的样品或产品的真正来源。对于后期检测植物育种中基因漂流数量、通过花粉散播的基因渗入等来说,筛选作为标记植物之标识物的本发明多核苷酸的存在与否是有价值的。
如此,且以低耗高效的方式,可将本发明的寡核苷酸探针试剂盒用来快速检测含有不同材料的不同样品或产品中多核苷酸的存在/不存在(或混合物情况下的相对数量)。可应用本发明测定的植物物种的例子包括林业物种,例如松树和桉树物种,其他树种,甚至农业和园艺植物。
就预期调节PCD和/或发育途径的应用而言,可将开放阅读框架以有义或反义方向插入遗传构建体中,这样与野生型生物中该多核苷酸拷贝数量和/或多肽的表达水平相比,具有遗传构建体的林业植物的转化发生多核苷酸拷贝数量或多肽表达水平的改变。转化含有有义方向开放阅读框架的遗传构建体将通常导致该选择多核苷酸编码的多肽表达水平的增加,而转化含有反义方向开放阅读框架的遗传构建体将通常导致该选择多核苷酸编码的多肽表达水平的降低。应用本领域技术人员共知的技术,可检测用含有有义方向或反义方向开放阅读框架的遗传构建体转化的林业植物该选择多核苷酸拷贝数量的增减或兴趣多肽表达水平的增减。这样可鉴定和分离具有预期改变的植物。总而言之,与相应的野生型生物的表达水平相比,兴趣多肽表达水平的增加或减少至少25%,效果明显。
用本发明遗传构建体转化靶生物导致靶生物多肽合成或含量或结构的改变,产生在PCD或发育途径范围不同于野生型植物的改变。本发明的方法涉及改变,通常增强和抑制林业物种中PCD。例如,用含有编码本发明多核苷酸编码多肽的开放阅读框架的遗传构建体转化靶生物,其中该开放阅读框架是有义方向的并且该多肽对PCD水平的起增加作用,通常在靶生物中产生该多肽的量或表达的明显增加,随后引起PCD的明显增加。类似地,用含有编码本发明多核苷酸编码多肽的开放阅读框架的遗传构建体转化靶生物,其中该开放阅读框架是有义方向的并且该多肽对PCD水平的起降低作用,通常在靶生物中产生该多肽的量或表达的明显降低,从而引起PCD的明显降低。用含有反义方向开放阅读框架或多核苷酸非翻译区的遗传构建体转化靶生物,依据该特异多肽的作用,通常产生PCD相应的增加或减少。将认识到用本发明的其它遗传构建体转化将产生多种多肽含量、组成和/或代谢的变化,该多肽在PCD和/或植物发育途径中起作用,因此产生该林业植物所含成分、组成和/或代谢的变化。
更具体地,本发明方法涉及通过如下措施选择性地增强林业植物或细胞群中的PCD,措施有:(1)导入或增加多核苷酸的拷贝数目,该多核苷酸含有的序列选自SEQ ID NOS:1-5、10、15、16、27-35、38-40、45、92-140、192-195和200所列举的该组序列以及这些序列的变体;(2)导入或增加多核苷酸编码的多肽表达水平或活化多肽,该多核苷酸含有的序列选自SEQ ID NOS:1-5、10、15、16、27-35、38-40、45、92-140、192-195和200所列举的该组序列以及这些序列的变体;(3)导入或增加多肽的表达水平或活化多肽,该多肽含有的序列选自SEQ ID NOS:46-50、55、60、61、72-79、82-84、89、143-191、196-199和201所列举的该组序列以及这些序列的变体;(4)降低多核苷酸的拷贝数目,该多核苷酸含有的序列选自SEQ IDNOS:6-9、11-14、17-26、36、37、41-44、90、91和204所列的该组序列及其变体;(5)降低多核苷酸编码多肽的表达水平或将其灭活,该多核苷酸含有的序列选自SEQ ID NOS:6-9、11-14、17-26、36、37、41-44、90、91和204所列的该组序列及其变体;或(6)降低多肽的表达水平或灭活多肽,该多肽含有的序列选自SEQ IDNOS:51-54、56-59、62-71、80、81、85-88、141、142和205所列举的该组序列以及这些序列的变体。本发明方法还涉及通过如下措施选择性地抑制林业植物或细胞群中的PCD,措施有:(1)导入或增加多核苷酸的拷贝数目,该多核苷酸含有的序列选自SEQ ID NOS:6-9、11-14、17-26、36、37、41-44、90、91和204所列举的该组序列以及这些序列的变体;(2)导入或增加多核苷酸编码的多肽表达水平或活化多肽,该多核苷酸含有的序列选自SEQ ID NOS:6-9、11-14、17-26、36、37、41-44、90、91和204所列举的该组序列以及这些序列的变体;(3)导入或增加多肽的表达水平或活化多肽,该多肽含有的序列选自SEQ ID NOS:51-54、56-59、62-71、80、81、85-88、141、142和205所列举的该组序列以及这些序列的变体;(4)降低多核苷酸的拷贝数目,该多核苷酸含有的序列选自SEQ ID NOS:1-5、10、15、16、27-35、38-40、45、92-140、192-195和200所列的该组序列及其变体;(5)降低多核苷酸编码多肽的表达水平或将其灭活,该多核苷酸含有的序列选自SEQ ID NOS:1-5、10、15、16、27-35、38-40、45、92-140、192-195和200所列的该组序列及其变体;或(6)降低多肽的表达水平或灭活多肽,该多肽含有的序列选自SEQ ID NOS:46-50、55、60、61、72-79、82-84、89、143-191、196-199和201SEQ ID NOS:51-54、56-59、62-71、80、81、85-88、141、142和205所列举的该组序列以及这些序列的变体。
在遗传构建体中,按有义或反义方向插入部分本发明开放阅读框可抑制参与PCD或选择发育途径多核苷酸的表达。这些部分不必要是全长多核苷酸,但优选包括本发明多核苷酸的至少25个残基,更加优选地,至少50个残基。更长部分,甚至也可以应用与完整开放阅读框对应的全长多核苷酸。只要有导致靶多核苷酸抑制的足够序列相似性,开放阅读框的该部分没有必要和内源序列精确相同。因此,从一个物种得到的序列可用以抑制不同物种中基因的表达。
依据另一个实施方案,本发明的遗传构建体包括的多核苷酸包含编码本发明多核苷酸编码之多肽的多核苷酸的非翻译区域,或者与该非翻译区域互补的多核苷酸。在这些构建体中能够有效利用的非翻译区的例子有内含子和5’端非编码引导序列。用这样一个遗传构建体去转化靶植物通过共抑制过程通常可引起该多肽表达水平的降低,类似此方式的讨论见,例如,Napoli等在植物细胞(Plant Cell)2:279-290,1990和Carvalho Niebel等在植物细胞(Plant Cell)7:347-358,1995。
或者,通过在核酶构建体中插入适当的序列或亚序列(例如DNA或RNA)可以实现调节(McIntyre CL和Manners JM,“抑制在烟草中过氧化酶基因表达的方法:设计高效核酶”转基因研究(Transgenic Res).5(4):257-262,1996)。核酶是合成的RNA分子,它包括与两个区域互补的杂交区域,两个区域中的任一个都包括本发明的一个多核苷酸编码mRNA分子中至少5个相邻的核苷酸。核酶拥有高度特异性的核酸内切酶活性,它自我催化断裂mRNA。
本发明的遗传构建体还包括基因启动子序列和基因终止序列,其可操纵地与该多核苷酸连接并能够控制多肽的表达。基因启动子序列通常位于待转录多核苷酸的5’末端并用于启动多核苷酸的转录。基因的启动子序列通常发现于基因的5’非翻译区域,但它们可存在于开放阅读框架的下游或内含子内(Luehrsen KR,Mol.Gen.Genet(分子基因遗传学),225:81-93,1991);或者存在于编码区内,例如存在于植物防护基因中(Douglas等,EMBO J.10:1767-1775,1991)。
在本发明遗传构建体中可有效应用的多种基因启动子序列是本领域共知的。基因启动子序列以及基因终止序列,对靶宿主来说可以是内源的或外源的,只要该启动子在靶宿主内有功能的话。例如,当靶生物为植物时,所用启动子和终止序列可以来源于其他植物物种、植物病毒、细菌质粒等。在优选的实施方案中,该基因启动子和终止序列与导入的那些多核苷酸(的启动子和终止序列)相同。
影响启动子选择的因素包括构建体所需组织特异性,以及转录和翻译的时间。例如,在本发明中可以有效应用组成型启动子,例如35S的花椰菜花叶病毒(CaMV35S)启动子,其具有或不具有增强子如KozaK序列或W增强子,以及根癌土壤杆菌(Agrobacterium tumefaciens)胭脂碱合酶终止子。应用组织特异性启动子将仅在兴趣组织中导致期望的有义或反义RNA的产生。应用携有可诱导基因启动子序列的遗传构建体,可通过外源刺激,例如光、热,缺氧应激,以及改变营养条件等调节RNA聚合酶的结合和起始效率。在转化细胞发育的某一特定时间时间调控的启动子可以用来影响对RNA聚合酶结合和起始效率的调节。优选地,应用来源于所讨论基因的原有启动子,或来源于待转化生物如桉树或松树中特定组织靶向基因的启动子。可有效应用于本发明中的基因启动子的其他例子包括甘露氨酸合酶(mas)、章鱼氨酸合酶(ocs)及那些由Chua等(科学(Science)244:174-181,1989)综述的启动子。
基因终止序列,位于转录多核苷酸的3’端,可以和基因启动子序列来源于同一基因启动子序列,也可来自不同的基因启动子序列。本领域共知的许多基因终止序列可以有效应用于本发明中,例如根癌土壤杆菌胭脂碱合酶基因3’端。然则,优选的基因终止序列是那些来自原有多核苷酸或者来自待转化靶物种的序列。
本发明的遗传构建体还可以包括在靶细胞例如,林业植物细胞中有效的选择标记,以促进含有本发明构建体之转化细胞的检测。这些本领域共知的标记物,一般对一种或多种毒素带有抵抗性。一个这种标记物的例子是NPTII基因,其表达导致对卡那霉素或潮霉素的抗性,中等浓度的这些抗生素对植物细胞通常是有毒的(Rogers等,见Weissbach A和Weissbach H编,植物分子生物学方法(Methods forPlant Molecular Biology),Academic Press Inc.:SanDiego,CA,1988)。由此,可以通过它们在含有该讨论的抗生素的培养基中生长的能力鉴定转化的细胞。或者,可以通过本领域共知的其他技术例如Southern和Western印迹测定转化细胞中该期望构建体的存在。
操作性连接本发明的遗传构建体成分的方法是本领域共知的,包括使用含有所述一或多个限制性内切酶位点的合成的连接物,例如,据Sambrook等所述的,分子克隆:实验室手册,CSHL Press:冷泉港,NY,1989。可以将本发明的DNA构建体连接到一个拥有至少一套复制系统的载体中,例如大肠杆菌,而在每一个操作之后,可以克隆、测序该产生的构建体,并可以测定操作的正确性。
可用本发明的遗传构建体转化林业植物,包括裸子植物(如,欧洲赤松(Aronen,Finnish Forest Res.Papers,Vol.595,1996)、白云杉(Ellis等,生物技术(Biotechnology)11:84-89,1993)和落叶松(Huang等,In Vitro Cell 27:201-207,1991)。在一个优选的实施方案中,将本发明的DNA构建体用以转化木本植物,此处定义为多年生树或灌木,其随着木组织的增加每年茎的直径也有增加。优选靶植物选自桉树和松树物种,最优选地是选自巨桉和辐射松。用本发明DNA构建体有效转化的其他物种包括,但不局限于:松树,如美国短叶松(Pinus banksiana),土耳其松(Pinus brutia),加勒比松(Pinus caribaea),沙松(Pinus clausa),小干松(Pinuscontorta),大果松(Pinus coulteri),短叶松(Pinus echinata),Pinus eldarica,湿地松(Pinus ellioti),约弗松(Pinusjeffreyi),糖松(Pinus lambertiana),小白松(Pinus monticola),欧洲黑松(Pinus nigra),长叶松(Pinus palustrus),海岸松(Pinuspinaster),西黄松(Pinus ponderosa),美加红松(Pinusresinosa),刚松(Pinus rigida),晚松(Pinus serotina),北美乔松(Pinus strobus),欧洲赤松(Pinus sylvestris),火炬松(Pinus taeda),北美二针松(Pinus virginiana);其他裸子植物,例如太平洋银枞(Abies amabilis),胶枞(Abies balsamea),白云杉(Abies concolor),巨冷杉(Abies grandis),洛杉矶冷杉(Abies lasiocarpa),红果冷杉(Abies magnifica),壮丽冷杉(Abies procera),美国扁柏(Chamaecyparis lawsonina),黄扁柏(Chamaecyparis nootkatensis),美国尖叶扁柏(Chamaecyparis thyoides),Huniperus virginiana,欧洲落叶松(Larix decidua),美洲落叶松(Larix laricina),日本落叶松(Larix leptolepis),西方落叶松(Larix occidentalis),新疆落叶松(Larix siberica),下延香松(Libocedrus decurrens),欧洲云杉(Picea abies),恩格曼氏云杉(Picea engelmanni),白云杉(Picea glauca),黑云杉(Picea mariana),锐尖北美云杉(Picea pungens),红云杉(Picea rubens),北美云杉(Piceasitchensis),花旗松(Pseudotsuga menziesii),Sequoiagigantea,北美红杉(Sequoia sempervirens),落羽杉(Taxodiumdistichum),加拿大铁松(Tsuga canadensis),异叶铁杉(Tsugaheterophylla),西美山铁杉(Tsuga mertensiana),北美香柏(Thujaoccidentalis),北美乔柏(Thuja plicata);及桉树,如Eucalyptusalba,Eucalyptus bancroftii,Eucalyptus botyroides,Eucalyptusbridgesiana,Eucalyptus calophylla,赤桉(Eucalyptuscamaldulensis),柠檬桉(Eucalyptus citriodora),Eucalyptuscladocalyx,Eucalyptus coccifera,Eucalyptus curtisii,Eucalyptus dalrympleane,Eucalyptus deglupta,Eucalyptusdelagatensis,Eucalyptus diversicolor,Eucalyptus dunnii,美丽花桉(Eucalyptus ficifolia),蓝桉(Eucalyptus globulus),Eucalyptus gomphocephala,Eucalyptus gunnii,Eucalyptushenryi,Eucalyptus laevopinea,Eucalyptus macarthurii,Eucalyptus macrorhycha,花皮桉(Eucalyptus maculata),Eucalyptus marginata,Eucalyptus megacarpa,Eucalyptusmelliodora,Eucalyptus nicholii,Eucalyptus nitens,Eucalyptusnova-anglica,Eucalyptus obliqua,Eucalyptus obtusiflora,Eucalyptus oreades,Eucalyptus pauciflora,Eucalyptuspolybractea,Eucalyptus regnans,Eucalyptus resinifera,大叶按(Eucalyptus robusta),野桉(Eucalyptus rudis),Eucalyptussaligna,Eucalyptus sideroxylon,Eucalyptus stuartiana,细叶桉(Eucalyptus tereticornis),Eucalyptus torelliana,Eucalyptus urnigera,Eucalyptus urophylla,Eucalyptusviminalis,Eucalyptus viridis,Eucalyptus wandoo,Eucalyptusyoumanni。
将遗传构建体稳定地整合进靶植物基因组的技术是本领域共知的。适宜转化植物的技术包括根瘤土壤杆菌介导的导入、电穿孔、原生质融合、生殖器官注射、未成熟胚胎注射、高速发射物导入等等。技术的选择取决于将要转化的靶植物。例如,可用例如Bevan在核酸研究(Nucleic Acid Res.)12:8711-8721,1984中所述的土壤杆菌属Ti质粒技术转化双子叶植物和某些单子叶植物及裸子植物。本发明遗传构建体导入的靶标包括组织,例如叶组织、弥漫性细胞、原生质、种子、胚胎、分生组织区域;子叶、下胚轴等。应用通过根瘤土壤杆菌的外发射诱导或体细胞胚胎发育方法是转化桉树和松树的优选方法。
通过标记,如上述卡那霉素抗性标记可以筛选基因组中整合了本发明遗传构建体的靶细胞。而后应用本领域内共知的技术,就可以在适宜的培养基中培养转基因细胞以再长出完整植株。对原生质而言,在适宜的渗透条件下其细胞壁可以重新形成。对种子或胚胎来说,则应用适宜的发芽或愈伤组织形成培养基。对外植体而言,则应用适宜的再生培养基。对多种植物来说再生的方法已经成熟。关于林业树林再生的综述,见Dunstan等,“木本植物的体细胞胚胎发育”pp.471-450,Thorpe,TA编“植物的体外胚胎发育”(当代植物科学和农业生物技术(Current Plant Science and Biotechnology inAgriculture)20卷)。云杉的特定再生办法讨论见Roberts等人,“云杉的体细胞胚胎发育”,pp.427-449,Redenbaugh K编,种子合成:农作物改良中合成性种子的应用(Synseed:application ofsynthetic seed to crop improvement),CRC出版社,1993。应用本领域共知的方法可有性或无性繁殖该产生的转化植物,以产生转基因植物的子代。
如上所述,通过合适启动子序列的选择,或通过筛选掺入进靶宿主基因组的多核苷酸的功能性拷贝数或整合位点,可控制靶植物细胞中RNA的产生。可以用本发明的一种以上的遗传构建体转化靶宿主生物,由此调节一种以上多肽的浓度或活性、影响一种以上组织或者影响一种以上的表达时间。类似地,可以组装遗传构建体,该遗传构建体含有本发明多核苷酸编码的一个以上的开放阅读框架或多核苷酸的一个以上的非翻译区域。本发明的多核苷酸也可以和其它编码多种多肽的已知序列联合应用。
另外,可将本发明的多核苷酸用作标记生物尤其植物的非破坏性标记物。作为异源的、非功能性的、无破坏性的标记物,可以将含有本发明多核苷酸的遗传构建体稳定地导入生物中。其后就可以通过测定材料样品中标记物的有无来鉴定该生物的起源或来源。也可以用本发明多核苷酸标记除植物以外的生物,包括有商业价值的动物、鱼、细菌和酵母。
可通过应用一系列传统技术通常包括核酸探针的应用来完成对标记物的检测。应用分枝的寡核苷酸可以有效增加分析探针存在的敏感性,如Horn,T,Chang,CA和Urdea,MS“在核酸定量分析中用作放大器的支链寡-脱氧核糖核苷酸的化学合成和描述”,核酸研究(Nucleic Acids Research.)25(23):4842-4849,1997中所述,能够对样品中少至50个DNA分子进行检测。
以说明的形式而非以限制的形式提供下述实施例。
实施例1
辐射松和巨桉cDNA克隆的分离和鉴别
如下所述应用非减数或减数方法构建和筛选辐射松和巨桉cDNA文库。应用Chang等在植物分子生物子报道基因(Plant MolecularBiology Reporter)11:113-116,1993中提供的方案,从植物组织中抽提mRNA。用Poly(A)快速mRNA分离试剂盒(Stratagene,La Jolla,California)或Dynal Oligo(DT)25珠从总的RNA制备物中分离mRNA。通过用逆转录酶合成的方法从纯化的mRNA构建了一个非减数cDNA文库,之后应用ZAP表达cDNA合成试剂盒(StratageneLa Jolla,California)或Superscript Choice System(Gibco BrlLife Technologies,Gaithersburg Maryland),根据制造商提供的方案,在Lambda ZAP中插入该产生的cDNA克隆。用Gigapack IIPackaging Extract(Stratagene),从5μl连接混合物中取1μlDNA样品包装该产生的cDNAs。用具有ExAssist辅助噬菌体(Stratagene)的XL1-Blue MRF’细胞和XLOLR细胞(Stratagene)进行文库的大量切除。切除的噬菌粒用NZY液体培养基(Gibco BRL,Gaithersburg,Maryland)稀释将其并涂布于含有X-gal和异丙基硫代-β-半乳糖苷(IPTG)的LB-卡那霉素琼脂平板上。
如下构建减数辐射松cDNA文库(应用晚期树木木质部[测试DNA]减去早期木质部[驱动DNA]或早期树木木质部[测试DNA]减去晚期木质部[驱动DNA]形成的)。根据Clontech PCR-SelectTMcDNA消减试剂盒(Clontech Laboratories Inc,Palo Alto,California)中制造商的说明将Dynal珠(见上)分离的mRNA用于产生cDNA。用限制性内切酶RsaI酶切该测试和驱动双链cDNA制备物,然后只将该酶切的测试cDNA群体用来产生两个不同的测试cDNA群体,每一个由不同的衔接子连接。应用联合的t测试和驱动cDNA全体进行第一轮杂交,使差异表达序列均等和富集。然后是产生PCR扩增模板的第二轮杂交。应用抑制PCR,差异表达的序列得以指数扩增。然后将该产生的cDNA全体用于第二轮PCR扩增以去除背景并进一步富集差异表达的序列。根据Khan等人,TIG 10:7,July 1994:或Hadjeb和Berkowitz,生物技术(Biotechniques),January 1996)的方法将PCR产物与T-tailed pBluescript II SK+相连。用电-感受态XL1-蓝大肠杆菌细胞与重组质粒和铺至含有X-gal和IPTG的LB-氨苄青霉素板上的细胞进行电穿孔。
在具有适宜抗生素的NZY肉汤中培养含有cDNA插入片段的克隆并以碱性裂解和聚乙二醇(PEG)沉淀的方法纯化cDNA。将1%琼脂糖胶用于检测测序模板的染色体的污染。根据制造商的方案应用TurboCatalyst 800机器(Perkin Elmer/Applied Biosystems Division,Foster City,California)制备染色引物序列。
应用Perkin Elmer/Applied Biosystems Division Prism 377测序仪获得阳性克隆的多核苷酸。cDNA克隆开始是从5’端进行测序,有些情况下也可以从3’端进行。对某些克隆,用亚克隆片段、核酸外切酶III缺失,或通过应用为识别兴趣基因区域而设计的基因-特异引物直接测序来获得其内部序列。应用限制性作图的标准程序进行亚克隆,并亚克隆到pBluescript II SK+载体及其他标准的测序载体。
该测定的cDNA序列,包括本发明的多核苷酸,与EMBL数据库(更新至1998年8月末)中的已知序列进行对比和匹配。具体地,应用按上述参数设定的BLASTN程序2.0.4版本〔1998年2月24日〕和版本2.0.6〔1998年9月16日〕将SEQ ID NOS:1-45、90-195、200、202、204和206确定的多核苷酸与EMBL数据库中的多核苷酸进行对比。具体地,应用得出E值和同一性百分比的BLASTN判定匹配和相似性所需的运行参数如下:Unix运行命令:blastall-p blastn-dembldb-e 10-G 0-E 0-r l-v 30-b 30-i queryseq-O结果。将冗余序列的多重匹配用以建立起可靠的同一性序列。基于来自于其他植物或非植物物种的已知序列的相似性,本发明鉴定为SEQ IDNOS:1-45、90-140、192、195、200和204的分离的多核苷酸,鉴定为编码多肽,其与上述表1中所示的多肽具有相似性。
用计算机程序BLASTN将分离的cDNA序列与EMBL DNA数据库中的序列进行对比。用计算机程序BLASTP将相应的推定多肽序列(在每六个阅读框架中翻译成蛋白质的DNA)与SwissProt数据库中的序列进行了对比。在1999年3月将SEQ ID NOS:1-45、90-195、200、202、204和206提供的DNA序列和EMBL DNA数据库中的序列进行了对比(用BLASTN)并(用BLASTP)对比SEQ ID NOS:165-304提供的氨基酸序列和SwissProt数据库中的序列。应用TBLASTX程,对照所有6个阅读框架(双链)中动态翻译EMBL DNA数据库,进行该氨基酸的分析。用TBLASTX程序将序列SEQ ID NOS:1-45、90-195、200、202、204和206多核苷酸的六读框翻译分析(结果)与EMBL数据库中多核苷酸的六-读框翻译也进行了对比和匹配。BLASTN多核苷酸分析
应用上述计算机程序BLASTN测定SEQ ID NOS:1-7、10-18、20-31、35-40、43、44、90-94、98-107、109、110、112-118、120、121、123-140、192-195、200、204和206的cDNA序列,与EMBL数据库中的序列进行对比,其按上述方法测得的同一性小于50%。用BLASTN按上述办法测定SEQ ID NOS:8、9、33、34、41、42、96、108和111的cDNA序列,与EMBL数据库序列的相比,其按上述方法测得的同一性小于75%。用上述BLASTN测定SEQ ID NOS:32、95、97、119和122的cDNA序列,与EMBL数据库的序列相比,其按上述方法测得的同一性小于90%。用上述BLASTN测定SEQ ID NO:9的cDNA序列,与EMBL数据库的序列相比,其按上述方法测得的同一性小于98%。BLASTP氨基酸分析
用上述的BLASTP计算机程序测定SEQ ID NOS:46,48-50,55,58,60,61,80,81,87-89,141,142,144,156,157,160,161,173,174,177,179,180,182-191,198和205,的预测的氨基酸序列,与SwissProt数据库中序列相比,其按上述方法测得的同一性小于50%。用上述的BLASTP计算机程序测定SEQ ID NOS:49,57,59,66,67,72,82-84,143,145,149,150,152-155,159,162-173,178,181,196,197,199,和201,的预测的氨基酸序列,与SwissProt数据库中序列相比,其按上述方法测得的同一性小于75%。用上述的BLASTP计算机程序测定SEQ ID NOS:51-54,56,62-65,68-71,73,74,79,146,147,151,158,和176,的预测的氨基酸序列,与SwissProt数据库中序列相比,其按上述方法测得的同一性小于90%。用上述的BLASTP计算机程序测定SEQ ID NOS:75、78、85、86和148的预测的氨基酸序列,与SwissProt数据库中序列相比,其按上述方法测得的同一性小于98%。TBLASTN和TBLASTX分析
测定SEQ ID NOS:46,47,49,50,58,60,61,80,83,87,89,141,157,160,161,174,175,177,179-180,182-191,和197-199,的预测氨基酸序列,与EMBL数据库所有6个阅读框架(多核苷酸的双链)中动态翻译的预测氨基酸相比,其按上述方法测得的同一性小于50%。测定SEQID NOS:48,55,57,59,66,67,69,71,72,81,82,88,142-145,149,150,152,153,155,156,162-165,167-172,176,178,181和205,的预测氨基酸序列,与EMBL数据库所有6个阅读框架(多核苷酸的双链)中动态翻译的预测氨基酸相比,其按上述方法测得的同一性小于75%。测定SEQ ID NOS:51,52,56,62-65,68,73,74,84,146,147,151,154,158,159,166,173,和196,的预测氨基酸序列,与EMBL数据库所有6个阅读框架(多核苷酸的双链)中动态翻译的预测氨基酸相比,其按上述方法测得的同一性小于90%;应用TBLASTN程序版本2.0.6[9-16-1998]测定SEQ ID NOS:53、75、78、79、85、86和148的预测氨基酸序列,与EMBL DNA数据库所有6个阅读框架(多核苷酸的双链)中动态翻译本相比,其按上述方法测得的同一性小于98%,该程序如下设定参数:Unix运行命令:blastall-blastn-d embldb-e10-GO-EO-v30-i queryseq-oresult。
最后,应用TBLASTX程序版本2.0.6[9-16-1998]将SEQ ID NOS:1-45、90-140、192-195、200、204和206的多核苷酸的六框架翻译本与EMBL中多核苷酸的六框架翻译本比较并配对,该程序该程序如下设定参数:Unix运行命令:blastall-blastn-d embldb-e10-GO-EO-v30-i queryseq-o resul t。应用计算机程序TBLASTX测定SEQID NOS:1-8、10、12-16、18、20-22、24-27、31、34-40、42-45、90-140、192-195、204和206的多核苷酸的翻译本,与EMBL数据库多核苷酸翻译本相比,其按上述方法测得的同一性小于50%。应用计算机程序TBLASTX测定SEQ ID NOS:17、28-30和41的多核苷酸的翻译本,与EMBL数据库多核苷酸翻译本相比,其按上述方法测得的同一性小于75%。应用计算机程序TBLASTX测定SEQ ID NOS:11、19和23的多核苷酸的翻译本,与EMBL数据库多核苷酸翻译本相比,其按上述方法测得的同一性小于90%。应用计算机程序TBLASTX测定SEQID NO:9的多核苷酸的翻译本,与EMBL数据库多核苷酸翻译本相比,其按上述方法测得的同一性小于98%
实施例2
应用DADI基因修饰烟草用辐射松DADI基因转化烟草植物
应用发表的方法(见An等,“双载体”(Binary vectors),见Gelvin SB和Schilperoort RA编,植物分子生物学手册(PlantMolecular Biology Manual),Klumer Academic Publishers:Derdrecht,1988)通过直接转化构建包括含有辐射松DADI(SEQ IDNO:8)编码区之多核苷酸的反义核苷酸的遗传构建体,并将其插入根瘤土壤杆菌(agrobacterium tumefacien)内。该反义核苷酸的核苷酸序列见SEQ ID NO:206。植物转化的一般方法见Horsch N、FryJ、Hoffman N、Eichholtz N、Rogers S和Fraley R,“将基因转移进植物的简单和一般方法”,Science 227:1229-1231,1985所述。通过PCR扩增该cDNA的开放阅读框架制备反义DNA构建体,然后纯化并将PCR产物克隆入pART27。而后将限制性内切酶NatI酶切的质粒和35S启动子-插入-OCS 3’UTR克隆进植物表达载体pART27(见Gleave A,“具有益于将克隆DNA高效整合入植物基因组T-DNA组织结构的多能双载体”,植物分子生物学(Plant Mol.Biol.),20:1203-1207,1992)。用限制性内切酶酶切及DNA序列测定来证实转基因构建体的存在、完整性和方向。
应用Horsch等人的方法(Science 227:1229-1231,1985)用该反义构建体转化烟草(Nicotiana tabacum cv.Samsan)叶切片。建立该有义构建体的多个独立的转化的植物品系应用SOUTHERN印记试验来证实含有适宜基因构建体的转化植物。
图1示从7个DAD1转基因烟草品系(泳道1-7)及非DAD1对照烟草植物(泳道8)分离的基因组DNA。正如所见到的,植物1-7含有与松树DAD1序列(最后冲洗条件:1×SSC、0.1%SDS 65℃)杂交的DNA,而对照烟草植物不含有该DNA。这证实成功地将与DAD1对应的反义多核苷酸转化进入靶植物中。
从7个DAD1转基因烟草品系和对照(不含DAD1)烟草品系中分离总RNA。应用Northern印记试验分析该RNA样品以判定每品系中的表达水平。将mRNA与松树DAD1探针杂交(最终冲洗条件:1×SSC、0.1%SDS 65℃)。图2示松树DAD1反义mRNA出现于7个DAD1转基因烟草品系(泳道1-7)中,,但是不出现于对照烟草品系(泳道8)中。
实施例3
在植物中单一序列标记物存在/缺失的证实
应用在烟草未发现的单一标记物序列来产生转基因植物。从辐射松SEQ ID NO:202和巨桉SEQ ID NO:203中分离该插入的单一标记物序列。应用已发表的方法(见An G,Ebert PR,Mitra A,Ha SB,等,“双载体”,见Gelvin SB和Schilperoort RA编,植物分子生物学手册,Klumer Academic Publishers:Derdrecht,1988)通过直接转化将该单一标记物序列插入根瘤土壤杆菌LBA4301(由Dr.C.Kado,University of California,Davis,CA惠赠)内。通过限制性酶切和DNA测序证实土壤杆菌转基因构建体中该单一标记物序列的存在和完整性。
应用HORSCH等人的方法(科学,227:1229-1231,1985)转化烟草(Nicotiana tabacum cv.Samsan)叶切片。为所用的每个单一序列标记物建立3个独立转化植物品系。应用缺少单一序列标记物的空基因转移载体建立两个空载体对照植物品系。
应用Southren印记分析来分析该序列标记物的单一性以验证该序列标记物存在于植物基因组中。如果该序列标记物是单一的,因此将其用作标签,那么,该序列标记物将显然不存在于未标记的植物中并将明显存在于已经标记的植物中。在本实施例中,该单一标记物存在于该单一序列标记物转化的转基因植物中。
应用Murry和Thompson的溴棕三甲铵(CTAB)提取方法(核酸研究8:4321-4325,1980)从空载体转化的对照植物和单一序列标记物转化的植物中制备基因组DNA。在用松树单一序列标记物(SEQ IDNO:202)转化植物的情况下应用限制性酶EcoRI酶切此DNA标本;在用桉树单一序列标记物(SEQ ID NO:203)转化植物的情况下应用限制性酶XbaI酶切此DNA标本。用1%的琼脂糖胶溶解在限制性酶切液中产生的DNA片段。
在琼脂糖胶电泳步骤之后,应用Southern(分子生物学杂志(J.MOL.BIOL.98:503-517))建立的方法将该DNA标本转移至Hybond-N+牌尼龙膜(Amersham Life Science,Little Chalfont,Buckinghamshire,England)上。用放射性标记的探针检测尼龙膜上上述的单一序列标记物并在严格条件下(最后冲洗:0.5×柠檬酸钠盐缓冲剂加0.1%十二烷基硫酸钠(SDS),15分钟,65℃)冲洗。应用自动X射线照像术检测该基因组DNA标本中探针与互补序列的杂交。
结果见图3和4。
图3示在Southern印记分析中应用来源于松树序列标记物(SEQID NO:202)的探针检测到的杂交模式。泳道A-B含有来自空载体转化的对照植物的DNA标本,泳道C-E含有来自SEQ ID NO:202转化植物的DNA。在泳道A-B中没有杂交,表示在空载体转化的烟草植物中不出现SEQ ID NO:202;即,SEQ ID NO:202是烟草植物明确标记适宜的单一标签。在泳道C-E中有明显的杂交,表明通过转化接受SEQ ID NO:202的植物已经明显和明确地标记有SEQ ID NO:202所含的单一序列。
图4示在Southern印记分析中应用来源于桉树序列标记物(SEQID NO:203)的探针检测到的杂交模式。泳道A-B含有来自空载体转化的对照植物的DNA标本,泳道C-E含有来自SEQ ID NO:203转化植物的DNA。在泳道A-B中没有杂交,表示在空载体转化的烟草植物中不出现SEQ ID NO:203;即,SEQ ID NO:203是烟草植物明确标记适宜的单一标签。在泳道C-E中有明显的杂交,表明通过转化接受SEQ ID NO:203的植物已经明显和明确地标记有SEQ ID NO:203所含的单一序列。
数据明显证实了明确标记转基因物质的目的说明书中所示序列的实用性。从大量的可能标签中选择单一序列,其在待标记的生物的基因组中不存在。将该标签插入待标记的生物的基因组中并很好建立DNA检测方法,该方法用于清楚检测用作标签的该单一序列标记物。
由于在该实施例中序列-特异性检测方法的应用,在说明书中所公开发明的用户很可能从已公开的列表中,发现将用于标记任何给定生物的序列标记物,并得到证明标记生物仅能通过向待标记生物的基因组精确地加入该单一序列才能获得给定的标记物的明确方法。如果本发明的使用者将给定生物中所用标签的精确序列作为秘密予以保持,那么应用本实施例中表明标签检测技术,可以明确解决有关该生物起源和历史的任何争论。
在所附的序列表给出SEQ ID NO:1-206。所附的序列表中所用核苷酸序列的代码,包括符号“n”,与WIPO标准ST.25(1998),附录2,表1相一致。
此处所引述的所有文献,包括专利文献和非专利出版物,在此完整引用供作参考。尽管在前述的说明书中,就一些优选实施方案对本发明加以描述,为了说明目的许多细节也得以阐明,但对本领域内技术人员来说显而易见的是,由本发明容易获得其他的实施方案,并且在不脱离本发明基本原则情况下,可将此处描述的某些细节进行大量的改动。
序列表
<110>Flinn,Barry
Lasham,Annette
<120>影响程序化细胞死亡的成分及其在林业植物发育改良中的应用
<130>1022PCT
<160>206
<170>FastSEQ for Windows Version 3.0
<210>1
<211>860
<212>DNA
<213>辐射松(Pinus radiata)
<400>1aatgcgtcct cgcagcgttt ttcaccttcg aaaaccattg cgtgttgtaa aagcaagctg 60cgcccggacg gatttcgtcg gaactcagaa gctttttgag ttttcggatt catatattca 120tcggaagtgc ttcccactgg aggaaattga aaccacgttg cagcctggca tttttgcagg 180gcatgatagg gctgaggagc tcagaacggc taattgaaag agatttcaga ggataaattc 240tgtggtttca atttgataca ggatgaaaat gagtagtttt aattaccctc ctcaggacga 300tggcgagccc caatatgcta gctcgggcag tggtgagaat cacatgcctg atgatgatga 360ccccaactgg ggcgatggtt ataaagttta cccacagcca aatcaaggtg aggcccaaga 420tcagcccgaa tatgcaggct tcaatgagga ttaccaggag cagaaaaatg acatctatgg 480taaaatcatg ctatctggca tactagtgtt tatttttatt attttgttag ccattttgct 540gcacgtttat gccagatggt tttggaggcg gtctgctcga ttccccaacc ggaatcgacg 600gagatcatct tctatccgtc atcggtttaa ttttatagaa gaagaaccgg tatggctgcg 660gaacgtgggg ttgcaatctg ccgtattaga gacacttccc atctttgtgt ataaatcaca 720ggatttcaca gatgggctgg agtgcgcagt gtgtctgtgc gaattcgagg agaatgagat 780agctcggctt ctgcccaatt gcaggcacaa ttttcatgtc gagtgtattg acatgtggtt 840tcgttcgcat tccacttgcc 860
<210>2
<211>367
<212>DNA
<213>辐射松(Pinus radiata)
<400>2ccggctggtg ggcgactgca acgccatggg cgacgactac ggcggcggct atcccaacac 60caaattcccc gacgacgggt cgtccaacgc ctacgccctc aacggcagga tcatgctcgc 120cgccatcatc gtcctcttct tcgtcgtcat catcatgatc tccctccacc tctacgcccg 180ctggttcctc ctccgccgcc agcagcgccg ccgcttcctc cgccgcaacc gcctcaaccg 240ccgcacccag atcgtcttct acgccgactt ccccgccccc caggcctccc gcggcctcga 300ctcctccgtc ctcaagtccc tccccgtctt caccttctcc tcctccgccg ccgccgccgc 360cgccgcc 367
<210>3
<211>330
<212>DNA
<213>辐射松(Pinus radiata)
<400>3gcaacgccat gggcgacgac tacggcttcg gcgatcccaa caccgaattc cgcggcaacg 60ggcagtccaa cgcctacgcc ctcaacggca ggatcatgct cgccgccatc atcgtcctct 120tcttcgtcgt catcatcatg atctccctcc acctctacgc ccgctggttc ctcctccgcc 180gccagcagcg ccgccgcttc ctccgccgca accgcctcaa ccgccgcacc cagatcgtct 240tctacgccga cttccccgcc ccccaggcct cccgcggcct cgactcctcc gtcctcaagt 300ccctccccgt cttcaccttc tcctcctccg 330
<210>4
<211>315
<212>DNA
<213>辐射松(Pinus radiata)
<400>4gaacaagact gtgattgagt ccctcccttt cttcaggttc tcctcgctca agggatcgaa 60acaagggcta gagtgcgcgg tgtgcttgtc caagttcgaa gacattgaga ttctccggtt 120actccccaag tgcaggcatg cattccacat cgattgcatc gattattggc ttgagaagca 180ctcaagctgc ccgctctgcc ggcacaaagt cagtgccgag gacccagcaa atttcaccta 240tacgaatagc atgaggctga tgagccaatc tgatatgaga caagattcca acttggagct 300gttcgttcag agaga 315
<210>5
<211>483
<212>DNA
<213>辐射松(Pinus radiata)
<400>5agctagcagt gctctgccat atttgcagag taggctatca aataatttga gggccgagac 60caaccccatt tgaaaggaaa tttctgtggc ttcaacatga gttcggttag cgaaacccat 120gaaccgcctc agtatgggag cgcgcagggt tatgtcatca gtggcaaaat catgttgtcg 180gcaataatat gtctttttgt tgtggtgttg ctcatgtttt tgctgcacct ttatgccaga 240tggatttggc gacactctgc taggttttcc cgacggaatc gacgcagatc agcttctagg 300cgtcgccggc ttcgtttctc gggacaagta ccggcgagtc tccagaatac ggggttggat 360tcttcgatat tgcagactct gcccatgttt gtgtataaat cccaagattt catcgatggt 420ctggagtgcg cagtctgtct gtgcgagttg gaggagaatg agaaagcccg gcttctgcca 480aat 483
<210>6
<211>600
<212>DNA
<213>辐射松(Pinus radiata)
<400>6caagacgaag acgacgacga cgacgagggt cgagaagcga agcaagggtt gcagcaggaa 60gaaagaatgg cgagatcgag cggcgacgac gctcaggctc tgttccactc gctccgatcc 120gcttacgccg ccactcccaa gaacctcaag atcatcgatc tgtacgtggc cttcgccgtc 180ttcacagcac tgattcaggt tgtttatatg gctctggttg gctctttccc tttcaattcg 240ttcctggctg ggggcctttc ttgtattgga acggccgtcc tggctgtttg tctccgtatc 300caagtcaaca aggagaacaa ggagttcaag gatttaccac ctgagcgtgc tttcgcagat 360tttgttctct gcaatttggt gcttcacttg gtgattatga atttccttgg ttaagctgga 420gccacgggtt ttgcaggatc tacatagctt gaggagtgat cgaatagtag taaaataact 480agttgccaat tcattttgct ttactggact gtgaggtgca aagctagtga tatgtgctga 540atgataggat tttgtggatt gactggggag cccaaattta tgttcttgtt tgaggtacac 600
<210>7
<211>778
<212>DNA
<213>辐射松(Pinus radiata)
<400>7caagacgaag acgacgacga cgacgagggt cgagaagcga agcaagggtt gcagcaggaa 60gaaagaatgg cgagatcgag cggcgacgac gctcaggctc tgttccactc gctccgatcc 120gcttacgccg ccactcccaa gaacctcaag atcatcgatc tgtacgtggc cttcgccgtc 180ttcacagcac tgattcaggt tgtttatatg gctctggttg gctctttccc tttcaattcg 240ttcctggctg ggggcctttc ttgtattgga acggccgtcc tggctgtttg tctccgtatc 300caagtcaaca aggagaacaa ggagttcaag gatttaccac ctgagcgtgc tttcgcagat 360tttgttctct gcaatttggt gcttcacttg gtgattatga atttccttgg ttaagctgga 420gccacgggtt ttgcaggatc tacatagctt gaggagtgat cgaatagtag taaaataact 480agttgccaat tcattttgct ttactggact gtgaggtgca aagctagtga tatgtgctga 540atgataggat tttgtggatt gactggggag cccaaattta tgttcttgtt tgaggtacac 600caagaaaaga atgatttttc ttctgccgaa aaaaaaaaaa aaacctttat ttgattagct 660tagttttgta tcatggttgt gcttagtctg ccgacatttg gtcctgtcaa ggatgtcaat 720gggctgtggc atgtcggatt ttataagtaa tccaaaattt tctgttcaaa aaaaaaaa 778
<210>8
<211>771
<212>DNA
<213>辐射松(Pinus radiata)
<400>8attttgtgac cgaaggccgg ggtgatcgaa acaagttgag agatccaagc gaaaatggga 60acctcaacag ctaaggatgc acaagttctc gttgcatcac ttcgatctgc atattctgca 120actcctacca agctgaagat catcgatctg tatgtggtct acgcagttct cacggcagtt 180gtgcaggttg tctacatggc aatagttgga tcatttcctt tcaatgcttt tctttcagga 240gttctatctt gcacggggtc agctgtgctt gcagtttgtt tgcggatgca agtcaacaaa 300gaaaacaagg aattcaagga tctccctcca gaaagagcat ttgcagattt tgttttgtgc 360aatcttgtac ttcacttggt gataatgaac ttcctaggtt agtggacaag gacttgcaga 420tttggaatga gagggtaccc atcacaaagc aacaaaagaa gaattggcta cttgcctttc 480cttgaaatat gtcgttgcta aggatttagt ggtagtcaaa tacacaaatg cctgaaattg 540gctcttcttg gttaatgtag ctcccttaat tttctcgtgc ttttgattct tggcgtagaa 600gacattgtac tcttacatat tgcaaacata aatataagct attgcattat tataccttcg 660tattgatcat ttcataatgt ggttcaggtc tttgaactct gttgtatggt ttgactcttg 720agaatcagtg ctgtcaataa gtatcaagca gaagtgatga gaaaaaaaaa a 771
<210>9
<211>424
<212>DNA
<213>辐射松(Pinus radiata)
<400>9gccaaggcca gggccggaga gaaatatcca atcaaggatg ggaagttcaa cagccaagga 60tgcgcacgta ctcgttgcct cgcttcgatc tgcatattct gcaactccca ccaaacttaa 120gattattgat ctgtatgtgg tttacgccat tctcacggca gttgtgcagg ttgtgtacat 180ggcaatagtt ggatcgtttc cttttaatgc ttttctttca ggagttctat catgcacagg 240gacagctgtg cttgcagttt gtttgaggat gcaagtgaac aaagaaaaca aggaattcaa 300ggatcttcct ccagaaagag catttgcaga ttttgtattg tgcaatcttg tacttcactt 360ggtgataatg aatttcctag gttaatggat aagcatttgt aaaaccggaa tcagagggtg 420gcat 424
<210>10
<211>689
<212>DNA
<213>辐射松(Pinus radiata)
<400>10cagaagcgct catgtttctc tctcatttca ttggagacat tcatcagcct ttgcatgtag 60gcttcactac agacagaggg gccaacgaaa ttgaggtccg ctggtacacc cggaaacaaa 120accttcacca tgtttgggat agtaatataa ttgagaccgc tgaagagaga tactacagct 180cagacacaga cggccttgtt gatgctatcc agcagaacat cacgaatgat tgggcagaag 240aagttaaagg ctgggagacc tgcagttcta ccaagccacc ttgcccagac atatatgcat 300ccgaaagtat cgccgcggcc tgtcagtggg catacaaagg tgtcagtgaa ggttcggtat 360tagaagatcc atatttcctg tcccgtttac ctactgttaa tcttcggtta gctaaagggg 420gagttcgact tgcagccact ctaaaccgca ttttcatgtg agtggcttca agttctggtt 480gaaaatatgg gactaaaagg aggacaaggt gaagatttaa ggcatattgg agtgacagag 540agctactcac agactacaat tgcagttcat gcttgtatag gtagtcatat gttcataccc 600tactgctttc catcttcttg aattgcccaa ctgttcagtg cgttgtcact aaataaagac 660attctgtcta ttaaaaaaaa aaaaaaaaa 689
<210>11
<211>484
<212>DNA
<213>辐射松(Pinus radiata)
<400>11acttcctgtt gttggagatc aaaaatgggt tatatggatc tgctccttca acgtaccaat 60ggcccctggg aagactcgtt ccatcgtttg cagtgctcga aacttctttc agttcacaat 120gccagggcca gcttggtggc aggtgatccc gaggtggcat gagcactgga cttcgaataa 180agtttatgat ggagatatga ttgtccttca gggacaggag aagatcttcc tctccaagtc 240gatggagggt caggaagacg taaatgagca atacacaaag atcacattta cacccactca 300agccgatcgg tttgtcctgg cattccgcaa ttggctaaga cggcacggga acagccaacc 360cgaatggttt ggctcgagca gtcaaaagcc tttgccatct accgtcttgt cgaaacgtca 420gatgcttgat cggttcgagc aacatactct caagtgctcg tcatgcagaa aagcctacga 480agca 484
<210>12
<211>769
<212>DNA
<213>辐射松(Pinus radiata)
<400>12atacaaatgg acgcattaac tcatggaact tctgtcggat tcatcacttt ctcgcccaaa 60attggcagcc aatgtaataa taaatccaag ggagactgca atttctcgtt cttgacagct 120aaagaacaat ctataagaag aagaagaaat aattttgcta caaggaggcg ggatttacat 180gtggtttctg caactgtagc tccgcccacc attcctggct cttcttccgc tgaagatttt 240gacaaagatc gtgaagcaga ggaggagagt gggaaattta tatggagaga tcattggtat 300cccgtttctt taattgaaga cctggacccc aagattccta cgcctttcca gctcttgggt 360cgcgagattg ttctctggca agatgccgag ggaaattgga aagccttcga ggacaagtgc 420ccccacagac ttgctcctct ctcggaaggg agattggatg agaatggatg gcttcaatgc 480tcctaccatg gctggtcttt caaggcagat gggtcatgtg ctcgaatccc gcaggccgca 540tccgaaggac ctgaatctcg ggccgcaagg tcacctcgag cttgtgcagt gagtttcccg 600acaacagtct ctcagggttt gctgttcgtg tggcccgacg agaatggctg ggaaagggcc 660tcaaagttcg agcccccatt gctacctgat gcatttagtg atccacgctt ctcaacggtc 720acaatacaaa gagatctgtt ctatggttat gatacattga tggaaaacg 769
<210>13
<211>482
<212>DNA
<213>辐射松(Pinus radiata)
<400>13gttcaaagcg ggtggtgatt tagccgaggg cgaagaggag gacgaagaag ggcttcgtaa 60caaacgtggc gattgatcct accttagcct gaaaatgctg tcaggaggct acgcaaccag 120atccgacact actactgtca acaacggatc cgctaatggc ccaataggaa gtgctccccc 180aagaattaac tcgatacaaa ataataatcc aggagctgtc aggcctggct ggggaaccat 240gccccttcac atgaatcctt atcatcccca atcaatgcct cttccgcccc ccaatggtat 300gcagggtcag cttgtgtgca gtggatgtag aactcttctt gtttatccgc aaggtgcacc 360aaatgtttgc tgtgcagtat gcaacacagt cactccagtt ccacctcctg ggacagaaat 420ggctcagcta atctgtggac gttgtcgtac attgctaatg tatgttcgtg gagcaactag 480tg 482
<210>14
<211>456
<212>DNA
<213>辐射松(Pinus radiata)
<400>14tttctgttcc accacaaaga atcgatggac caccgtcagg gacaacaccg tctacttcaa 60cgtcaatgcc ccaatctact caaactgtag tggttgaaaa ccccatgtcc gttgacgaga 120gtggcaaact ggtgactaat gtcgtcgtcg gcgtcacaac agagaaaagg tgatctattg 180ccaacagttg aatatggata tggagattgg ttgtgcatac cttgtgagaa agacattcac 240atcagtactg gagtccccag tacattcaga ttcctgtcgc tgtattgagg atcttgtaaa 300acttcgtctt catttgtttg gccctatggt acttgtagag tgaataaaag tcttgatatt 360cctacatgag cttggacaaa aaaggttatc tacttttatc agttgaatat ggagatggat 420gttggtttgt atatgatgtg gataatagca ttttat 456
<210>15
<211>1014
<212>DNA
<213>辐射松(Pinus radiata)
<400>15accaccagaa ataatgtgtc acctgcaact tctaccagtt cttctactgc ttttacaatc 60caaggcaatg tctatccaga tggactttat tatgtgtcaa tgctcattgg gaacccacca 120aagccctatc accttgatgt tgacacaggg agtgatttga cttggatcca gtgtgatgct 180ccttgcagaa gttgtgccaa gggaccacac gctctgtaca aacccaagaa aagccaactt 240gtatcctgca tggcgcctat gtgtgtgaat gtgcaagcag gtcacagcca tgaatgcaca 300agtacctctg aacagtgtga ctatgagata gagtatgctg atctgggatc ctctatggga 360gttcttgcga gagacaatat tagagtgtta ctgacaaatg gctctatagc tcgaacaaat 420tttgtgtttg gatgtgcata tgaccagcag ggttctcttg cagtttcacc tgcagtgaca 480gatggagtat tgggccttag cagtgctcag gttagtttgc catcacaatt agcaagtcaa 540ggtctgacta agaatgtgat tggtcattgt attgctggag atgaaagaga tgaaagaagt 600gggggttata tgttctttgg caatgatctt gtacctgtgt ggggaatgac atgggttccc 660atgtttggca agcctgcaat gaaattatat tccgtgggca gtgcaaatat gaaacttgga 720aacaggccgc ttgtttctaa tgacctaaaa aacaaattag gaggggtggt ttttgatagt 780ggtagctcct ttacatatct cacacagaca gcttacttcg catttgtctc agcagttaaa 840gagaatcttt ttggaggagg gttagtacag gatttgtcag ataaaacttt accgctgtgt 900tggcgggcaa gacatcctat caggtctata gcagatgtga aacctttctt caaaccattg 960actctggatt ttgggggcaa cccatggctt attaagacca aacagtttga catt 1014
<210>16
<211>530
<212>DNA
<213>辐射松(Pinus radiata)
<400>16ttcggcacga gattaagacc aaacagtttg acattccccc agaaggttac ttggtcatca 60gttctcatgg taatgtttgt ctgggaatcc tcaatggcag tgaagtgaac gatggcgcca 120caaatataat tggagacatt tctttgcaag gacaccttat tgtttatgac aatgtcaaga 180accagattgg ctgggtccat gcagattgcc acaagccacc taagatgatg aaggccttcc 240ccttttttaa gtaaaacaat gcagcaggaa ctggttcatg ctttgtaatg agcgatgcaa 300aattttgaac attttgtcaa atatacagaa gacagttgtt aatgtgcaaa tcgtattatt 360agtgttggtt atagcttata tgtacattat ttacatccac attttctcat atttggggat 420ctgtcgagct atcaacgagt cctttcacat atttaagtga aatcactatt ttaatatgtt 480tgtattgtat atattgcatc gatagtagaa tattatcata aaaaaaaaaa 530
<210>17
<211>1293
<212>DNA
<213>辐射松(Pinus radiata)
<400>17ctgactctct ctctctctgt tttgtctcct ccctcctctc tctcgttttc gcttcgtcgt 60gaacgcaccc acacgatctt ccattccctc aacaatgtcg actctcaccg tcccgcagcc 120actgccccct gtagccgatg actgcgagca gctccggaca gccttcgcag gatggggaac 180aaatgagaag ctgatcatat ccatattggg tcataggaat gcggcgcaga ggaagctgat 240tcggcaaacc tatgccgaga cttacggcga ggacctcctc aaggcattgg acagagaact 300taccaatgat ttcgagaggc tggtggtcct ttggtcactt gatccggctg aacgtgatgc 360gtacttggcg aatgaagcga cgaaaagatg gacttcaagc aaccaggttc tcatggaaat 420agcctgcacg aggtctccgc agcagttgct tatggcaaga caagcatatc atgcccgata 480caagaagtca atggaagagg acgtcgctca ccacacaact ggagattttc gtaagttgct 540ggtacctctt gggagctcct accgtaatga tggagatgag gtgaatatga ctttggcaaa 600agcagaggct aagatactcc acgagaagat ctcagagaag gcttatggcc atgaggatct 660cataaggatt ttggctacta ggagcaaagc acaggtcaat gctacgctga atcactacaa 720aaatgagttt ggaaatgata tcaacaagga tttgaaaact gatccaaaag acgcgttcct 780tactatactg agagctacag taaagtgcct gactcgccct gagaagtatt ttgaaaaggt 840tcttcgtcta gccatcaata agcgaggaac agatgaaggg gctctgacca gagtagttgc 900taccagggcc gaggttgaca tgaagtttat aagtgaggag taccagagga ggaatagcat 960ccctctcgat cgtgccattg tcaaggacac tactggagac tatgaaaaaa tgcttctggc 1020attgattggc cacgtcgagg cttgatttac aagtactcat gaagctatcc tggtggaggc 1080aatatctctg tttttggtgt ggtttgaggc atttctattt tccttgcttt ccaacaacgt 1140gtagttacca acatgcctcc ccagttgtca gttgtagcta tgcgaagcaa atacacttct 1200tataatggcg ttggtttatg tacttatgag aagtctttga ttttgatctt taatcaagac 1260tgctagtaag tgatcgtgaa aaaaaaaaaa aaa 1293
<210>18
<211>484
<212>DNA
<213>辐射松(Pinus radiata)
<400>18ggaagctgat tcggcaaacc tatgccgaga cttacggcga ggacctcctc aaggcattgg 60acagagaact taccaatgat tttgaggtct gatcttcttt aattatttgt attcatccca 120tggagacgcg tccctctttc tctcagatta atccatattc attccgtatc gtcagaggct 180ggtggtcctt tggtcgcttg atccggctga acgtgatgcg tacttggcga atgaagcgac 240gaaaagatgg acttcaagca accaggttct catggaaata gcctgcacga ggtctccaca 300gcagttgctc atggcaagac aagcatatca tgctcgatac aagaagtcgc tggaagagga 360cgtcgctcac cacacaactg gagattttcg taagttgctg gtacctcttg tgagctccta 420ccattatgat ggagatgagg tgaatatgac tttggcaaaa gcagaggcta agatactcca 480cgag 484
<210>19
<211>221
<212>DNA
<213>辐射松(Pinus radiata)
<400>19cgtacttggc gaatgaagcg acgaaaagat ggacttcaag caaccaggtt ctaatggaaa 60tagcctgcac gaggtctccg cagcagttgc ttatggcaag acaagcatat catgcccgat 120acaagaagtc gctggaagag gacgtcggtc accacacaac tggagatttt cgtaagttgc 180tggtacctct tgtgagctcc taccgttatg atggagatga g 221
<210>20
<211>789
<212>DNA
<213>辐射松(Pinus radiata)
<400>20atcgtcttcg gctcctcgcg atatcaccaa cttgcttccg cacagagaga gagagagaga 60gagagagaga gaatggcgac tatcgcggtg ccaccgtcgg ttccgtctcc ggctgaggat 120gccgagcagc tccaaaaagc tttcgcagga tgggggacga atgaagatct gatcatatcc 180atactgcctc acagaaacgc agcgcagcgg aaagtaatcc gacaaacata tgccgagaca 240tatggggaag atcttctcaa agcgcttgac aaggaactct ctagtgactt tgagagatct 300gtgcttctgt ggaccctgga tcctgcggag cgtgatgcat tcttgtccaa tgaagctacc 360aagagattga cttcgagcaa ctgggttctc atggaaattg cttgcacgag gtcttcaatg 420gagttattca tggtgaggca ggcctatcat gctcgttata agaaatctct tgaagaagac 480atcgcatatc acactactgg ggatttccgc aagctgcttg ttcctctggc aagtaccttt 540cggtatgagg ggcctgaggt gaacatgaca ttggcgagat cagaggctaa gatacttcat 600gagaagattc acgagaaggc ttacaatcat gatgagctca tcagaattgt tactacaaga 660agtaaagctc agcttaatgc aaccctcaat tactacaaca atgagtttgg gaatgccatc 720aacaaggatc tgaaggctga tccaaatgat gaatttctga aactgctgag atcagcaatt 780aagtgcttg 789
<210>21
<211>704
<212>DNA
<213>辐射松(Pinus radiata)
<400>21gttttgttga gctactagat tttagtaaat caagaattca tcagctataa attgaggcat 60tcgatttcag ttttagttac attttggtga agttggtcga cctgcattgc tgaagatatc 120gtgcgaagta tgtgatttgt cgagaagatg tcaacaatta tagtgccagt tccaataccg 180accccatctg aagactctga acgcctgagg aaggcttttg aagggtgggg cacaaatgag 240aagtcaatca tacaaatatt aggacataga actgcagcac aacgcaaagt aatccgtcaa 300agttattttc aactgtacga agaggatctc ttgaagcgat tagaatctga gctttcaagt 360gactttgaga aagctgtatt cctttgggta ctagatccag ctgaacgtga tgcggtcata 420tctcatggtg caataaagaa gtggaatgca aagaatatat cgcttttaga aatttccagt 480gctcgatctt cggctgaact attgatggtg aggcaagcat atcatattcg gtacaaaaag 540tccctcgaag aagacgtggc tgcacataca agtggaaact tccgtaagtt gctggtagca 600cttgtaagtt catatcggta tgaaggtccg gaagtggata tgcatttggc aagttatgaa 660gcaaagaagc taagtgaatc tataaccgag caaaaaagat aatt 704
<210>22
<211>1235
<212>DNA
<213>辐射松(Pinus radiata)
<400>22cacagctttc cttaaccgga gaagccatag atatgttttg aggttctggg ttttgtgtga 60agcataacac tgcgataatc gagaagagag agagcaatgt ctgtacaaag agcgttatca 120aacatagctg cgctagccat cagcgttgga acgggagtcg gcctcctaaa cgcgtcgctg 180tataccgtgg atggggggca caaagcggtc cttttcgaca gattcagagg cgtcctggac 240accaccgtgg gggaaggcac ccacttcctc attccatggc ttcagaaacc ctacatattc 300gagatcagaa cgaaaccccg ctccatcagc accatcacgg gcaccaagga cctccagatg 360gtgaacatct ctctgaggat acttgccagg cccaaagagg actcgttacc cgacatattc 420cagaggctcg gcctcgatta cgacgagaga gtgttgccct ccattgggaa tgaggtcttg 480aaggccgtcg tggcgcagtt caacgctgac cagctcttga ccgagaggcc aacggtttct 540gctctggtca gggaggctct gctgcatcgc gccaaagatt ttaacatttt gttggacgac 600gtggccatca cccatctgtc ctatggcccc gagttctcaa aagcagtgga gcagaagcag 660gtggcgcagc aggaagccga gaggtcgaag tttgtggttg ccaaagctga gcaggagagg 720agagctgccg tggtgagagc agagggggag agtgaggctg ctaagcttat ttctctggcc 780acgtctgctg cgggactagg gctaattgag ctcaggcgta tcgaggctgc caaggagatt 840gcttccaccc tttctcggag ccctaatgtg gtctacttgc cctctggcaa caacatgctc 900ttgggcttga acccttcgca ttgaacacga ggttactgat ccatcttaga tggtaaggag 960ttgatgatga gcccaagtca cggatgcgat cagcatgtcc agtgtttctt gataaatata 1020caagaaatta tttctgtata ttttttatgc ggtggaggtt ttttgggcaa agaatattca 1080tattaccgat ttagaactcg acaatatttc aaatatcaag atgcagagaa ataacatgct 1140taagccttct aggaaaaaat aagctttccg aaaaacggct ttgctcggat atatatgtta 1200ttaatttatc aaggttggga cgtcgacgcg gccgc 1235
<210>23
<211>497
<212>DNA
<213>辐射松(Pinus radiata)
<400>23ctctctctct atctccgctc gtcgatctgc gcttcgagga ggaagggagg cggggagaat 60ggggagcagc caggcggcgg tctcgttcct gacgaacgtg gcgcgggcgg cgttcggcct 120cggcgccgcc gggacggcgc tgaacgcgtc gctgtacacg gtcgacggcg gccagcgggc 180ggtgatcttc gaccggctcc gcggggtcat ggatgagacg gtcggggagg ggacccactt 240cctcgtcccc tggctccaga agcccttcat tttcgacatc cggacgaggc cgcacacctt 300ctcttccgtc tccgggacca aggatctcca gatggtcaac cttactctcc gagttctctc 360tcggcctcag gtctctcgtt tgccatatat cttccggcac ctgggtctcg agtatgatga 420gaaggtcctt ccgtcaatcg ggaatgaggt tttgaaagct gttgtcgccc aattcaatgc 480ggattcagct tcttact 497
<210>24
<211>527
<212>DNA
<213>辐射松(Pinus radiata)
<400>24gagtgttgcc ttcaatcatc cacgagacac tgaaagctgt ggtagcacag tacaatgcaa 60gtcaacttat cactcagaga gaggcagtaa gcagagaaat aaggagaatt ttgacggaga 120gagctgctaa tttttacatt gcattggacg acgtctctat aacaagcctt actttcggaa 180gagagttcac agctgccatt gaagcaaagc aagttgctgc tcaagaagca gaacgtgcca 240agtttgttgt cgagaaggca gaacaagata aaaagagtgc tatcattaga gctcagggag 300aagccacaag tgctcagctt attggtgaag ctatttctaa taatccagct ttcatcacgt 360tgagaaagat tgaggccagc cgggaaatag cacacactct ctctaactca actaatagga 420tcttcttgag ttctgattca ttactgctaa accttcagga tatgagcctg gacgatgcac 480acaagccacc acctaagcct aaaaagtgat gaacacaata aattatt 527
<210>25
<211>483
<212>DNA
<213>辐射松(Pinus radiata)
<400>25agattcggca cgagagcggt agctgcagcc ttccatcaaa tcaaggagac agagctcaac 60agatcagaga ttctatacaa gaagccgaga agaaacgggt tgggtagtca gagcaagaga 120aggaagatga attttaacaa tgtgagggtg cctggagggg gaggagctgc atgggcgcta 180actaaagccg tagtacttgg tggggctggg ctttacggtg cactcaacag tctctacaat 240gtcgagggag gtcacagggc cattgtcttc aataggatcg ttggtgtcaa ggataaggta 300tatcctgaag gcacacatct tatgattccc tggtttgata ggcctgtcat ctatgatgtc 360agagcacgtc ctcaccttgt agaaagcact tcaggcagcc gtgaccttca gatggttaag 420attggtctcc gagttcttac aagaccaatg ccagatcagt taccaacaat ttacaggccc 480ttg 483
<210>26
<211>384
<212>DNA
<213>辐射松(Pinus radiata)
<400>26cggcacgagt ctctctcccc cccccccccc cctccctcgc tcggtaggtt ccggctggtt 60cagttcagcg tcgactacga cccatttctc cgattcctcc gaccggtcgg cgagttctct 120cggggaatgg attttagaaa tgtcaaagtt ccaaaagtgc caggaggagg ggctacttct 180gctttgctca aactgggagt cataggtggc atagccctct atgcagccac aaacagtctc 240tacaacgttg agggaggtca tcgagccatt gtattcaatc ggctagttgg tgtaaaggac 300aaggtttatc ctgaagggac gcacataatg atcccgtggt ttgagaggcc agttatctac 360gatgtccgtg caagacctca ttta 384
<210>27
<211>768
<212>DNA
<213>辐射松(Pinus radiata)
<400>27gacggattct agcgagaagt cgtctgcctg ccggaatccg gtgactccga acgccctctg 60ccctgccgtg gtcttccgcg tcggcgcttc ttctcttctt ctgcgataag cggggtttgg 120agcgggaact ttccgttagg cttgccgttg agggtttggc agagcttgat ttcagtgaag 180tgaattatcg tctaaacggg aatacaacat gcgtcggttt ttttgctgta cttgctcaac 240agagggtgct cctgaacaac ctgaggccca tttcttgaat gcagctaaga acaatggaaa 300cgggttccag ggagactata agatgtctga gggtgcaaag agtggtccgc cacagaaaat 360agcacctatt gaagcacctg ctttgtcatt agaagaactg aaggaagcaa ctgataactt 420tggggcaaag gctttgattg gggaggggtc ctacggaaga gtttactatg caatgttaag 480tgatggtcaa cctgcagcaa tcaagaaatt ggatgtcaac agccagccag aggcaaattc 540cgaattctta gctcagattt caatggtctc aaggctgaag catgaccata tagttgagct 600ggtgggttac tgtgttgagg gaactctccg tgtattggct tatgagtttg caacaatggg 660ctctttacat gacattttac atggacggaa aggagtgcag ggggctcaac caggtccagt 720tctggattgg atgcaaaggg tgaaaattgc tgttggtgca gcaaaggg 768
<210>28
<211>932
<212>DNA
<213>辐射松(Pinus radiata)
<400>28gcaccgacgc caatcggtgc gctgccaaat tgaatgtggg actggaggct tacaagataa 60aaatgataat agatcagatg attggggtct ctgtaagctc tggaaaatat gagcatttgc 120aggtttatca agtgcgttac agtgggggat ggagctgtgg ggaagacatg cttgctcatc 180tcatatacaa gcaacacatt cccaactgac tacgtaccca cagtgttcga taacttcagt 240gcaaatgtgg tagttgatgg taacaatgtc aatctcggcc tctgggatac tgcagggcaa 300gaagactaca ataggttgag accactgagt tacaggggga cagacgtgtt cctcttggcg 360ttttccctga tcagcaaagc cagttatgaa aatgtttcca agaagtggat tcctgaactc 420aaacattatg tgccatctgt gccaattgtt ctcgtgggaa ccaaactaga tttacgagat 480gacaagcagt tttttagtga tcatcctggt gcagccccta taacaacagc ccagggagaa 540gagctaaaga accagattgg ggctgtagca tatattgagt gcagttctaa aacacagcag 600aacgtcaagg cagtttttga tgctgcaatc aattcggtcc ttcaactacc taaacctgtg 660aaacctcgaa agaaaaggca aacttgtgct gttctttgag gggaagttgg ggacttcttg 720aaaatatggt acctggaatc tggcttaaga tttcttgctt acatgtaatt tgatacattt 780tcttgaatct taacacagtt gccaatatct gcaaacacat tcagcttcag tgtttgctgt 840gtaaaaaaaa cagctgtatc tagatatcat ggatttggaa gtttatatta ttttttaatc 900attcggacag gtgattaaca aaatgtaatg tc 932
<210>29
<211>603
<212>DNA
<213>辐射松(Pinus radiata)
<400>29gctctggaaa atatgagcat ttgcaggttt atcaagtgcg ttacagtggg ggatggagct 60gtggggaaga catgcttgct catctcatat acaagcaaca cattcccaac tgactacgta 120cccacagtgt tcgataactt cagtgcaaat gtggtagttg atggtaacaa tgtcaatctc 180ggcctctggg atactgcagg gcaagaagac tacaataggt tgagaccact gagttacagg 240gggacagacg tgttcctctt ggcgttttcc ctgatcagca aagccagtta tgaaaatgtt 300tccaagaagt ggattcctga actcaaacat tatgtgccat ctgtgccaat tgttctcgtg 360ggaaccaaac tagatttacg agatgacaag cagtttttta gtgatcatcc tggtgcagcc 420cctataacaa cagcccaggg agaagagcta aagaaccaga ttggggctgt agcatatatt 480gagtgcagtt ctaaaacaca gcagaacgtc aaggcagttt ttgatgctgc aatcaattcg 540gtccttcaac tacctaaacc tgtgaaacct cgaaagaaaa ggcaaacttg tgctgttctt 600tga 603
<210>30
<211>472
<212>DNA
<213>辐射松(Pinus radiata)
<400>30caaccaaata taaccccaaa agcaggaaaa taaaagaagt tcacaagacc cagaattcca 60gaatttctgc tttttagatt caaggaaagc ttctttgttc ctttcttcct gggggtgttt 120cttcaatggc tgcgagtgcg tcaagattca tcaaatgtgt gacagttggt gatggggctg 180tgggcaagac ttgcatgctg atctgctaca ccagcaacaa gtttccaact gattatatac 240caacagtatt cgataacttc agtgcaaatg tagtagttga aggtaccact gttaacctgg 300gactctggga taccgctggg caagaagatt acaatagatt aagacccctg agctacagag 360gtgcagatgt ctttgtcttg gctttctctt tagttagtcg agctagttac gagaacatac 420ttaaaaagtg gatccctgaa ctccagcatt atgcaccagg aaatccctct gg 472
<210>31
<211>614
<212>DNA
<213>辐射松(Pinus radiata)
<400>31ccgagctccg ctttttataa aatcctttaa tcgcaagctg gcaagagttg tctctctcat 60tccagcccct cctccgtaaa gaaaatcaag aaagaaaatc cctctctctc tctagttcgc 120tctctctctc tctctctctc tctctcttgt gggtagtcag agtacgtttc cctccgcgga 180cgacttgctt ttcgctgcct ttccctggtt tcaatgcgcc ccatttcgcg gccacagagt 240ttcgccgtct ccgttcaaga ctagccggtg aagaggggcg gacgtttccg aagttcttgg 300gggggcaaga ggaggaggag gaggaggagg atatgagcgc gtcgaggttc atcaagtgcg 360tcaccgtcgg cgacggggcc gtcggcaaga cttgcatgct catctcctac actagcaaca 420ccttccccac ggactatgta ccaactgtgt tcgacaattt cagtgcaaat gtcgttgtgg 480atggaagcac tgttaacctg ggtttgtggg atacagctgg acaggaagac tataatagac 540taagacctct tagctaccgt ggggctgatg ttttcctgct cactttctct ctcattagca 600aggccagcta tgaa 614
<210>32
<211>408
<212>DNA
<213>辐射松(Pinus radiata)
<400>32atcaagtgcg tcaccgtcgg cgacggtgcc gtcggcaaaa cctgcctgtt gatttcttat 60accagcaaca ctttccccac ggactatgtg cccacggtgt ttgacaattt tagtgcaaat 120gtggtggtta atggaagtac tgttaatctg ggattgtggg atactgctgg acaagaggat 180tataacagat taagacctct aagttaccgt ggagcagatg tttttatact tgctttctct 240ctcataagca aggccagtta tgaaaacgtt tcaaagaagt ggattccaga attaaagcat 300tatgcacctg gtgtgccaat aattctcgtg ggaacaaagc tggatttgcg ggatgataag 360cagttcttta tagaccatcc tggtgcagtg cctattacga cccagcag 408
<210>33
<211>331
<212>DNA
<213>辐射松(Pinus radiata)
<400>33aagtgcgtga cggtcggcga cggggcggtg ggcaagactt gcttgctcat ttcctacacc 60agcaacacct tccccacgga ttatgtgccc accgtcttcg acaacttcag tgctaatgtg 120gttgtcaacg gaagcactgt gaacctggga ctgtgggata ctgcaggaca ggaggattac 180aacagactaa gacctttgag ttatcggggg gcagatgttt tcattctagc attctctctc 240atcagcaagg ccagctatga aaatgtctct aagaagtgga ttccggtgtt gaagcattat 300gcacctggtg tcccaattgt tcttgttggg a 331
<210>34
<211>466
<212>DNA
<213>辐射松(Pinus radiata)
<400>34gagtttttct gaagagtcag agcaaccatg agtactgcca gatttattaa gtgtgtgact 60gtgggggatg gtgctgtggg aaagacttgc atgcttattt cctacacgag caacacattt 120ccaacggact atgtaccaac agtgtttgat aacttcagtg caaatgtagt ggtggatgga 180agtacagtga atcttggcct ctgggacacg gcggggcaag aggattacaa caggctcagg 240cccctgagtt acagaggtgc agatgtcttc ctcctggctt tctccttgat cagcaaggcc 300agttatgaaa acattttcaa aaattggatt ccagaattga gacactacgc accatctgtg 360cctatcattt tggtgggaac aaaattagat ttacgagaag acaaacagtt ttttgcggat 420catcccggag cagccccaat ctcgacagct caaggtgaaa atttga 466
<210>35
<211>367
<212>DNA
<213>辐射松(Pinus radiata)
<400>35ctcgtgccgc gattgtctca ttcctcatca aatagttaaa accagcagga caatctgtag 60cagtgtagcc tggctgtttt ctcctacctc ctcttcaacc tcttctcttc tctctttctc 120ctccttcaaa gtgtccatca gtttcaactg tggctggaga gcttctcgca tagttccact 180ggggttttct tgtggggaag aaagatgagc gcctccaagt tcatcaagtg cgtcactgtc 240ggagatggag ctgtgggcaa gacttgcatg ctcatttgct acaccagcaa caagttccct 300actgattaca tacccacagt gtttgataac ttcagtgcaa atgtggctgt ggatgggaac 360atagtca 367
<210>36
<211>549
<212>DNA
<213>辐射松(Pinus radiata)
<400>36agagttaaag gatattgaca cagacacttt ctcctatttt gagggtctca tggaagagag 60ttcacttgta tcaagcattc aaatcctaga aaaagattat gaagatgcaa tatatgatag 120aggggaactt gacgagcgca tgtttgtgaa tgaagaggat agtttgtttg gttcagcaag 180caactcggga ggttctgtca ctgtgtctgg agtgaagaga aaatttgata gtatttcctc 240acctacaaag acaataacaa gtccaccatc tccacgaggc tctcctgttg catcccctgt 300gaaagagagc tctgccactg ccagtactaa gatgccaccc cctacaccag tgagcacagc 360aatgacaact gcaaagtggc ttcgaactgt tatagctcca cttcctccaa aaccttcttc 420agagcttggg cattttcttt catcatgtga cagggacatt accgcagatg taagtcaccg 480agcaagaata gtactagaag caattttcct agtagtcacc tggggaaaga tgtgttgtgg 540agcccagag 549
<210>37
<211>433
<212>DNA
<213>辐射松(Pinus radiata)
<400>37cacagatggt tttagctatt ttgagggtct catggaggaa acctccctga attcaagcat 60aagaatttta gagagtaact acatgaatgc aattcatgat agaggagaac tggatgagag 120gatgtttgtg aatgatgagg atagtttgtt tggttctgtc catgcatctg ttgattctgt 180caatatattg ggggcaaagc gaaagtatga ggctatctcc tctccaatga agagaataac 240aagtccatta tcttccccag tctctccatc agcttcagga acaatatatt caaatgtcaa 300gatgttgcca cctacaccag tgagcacaac aatgactact gcgaagtggc ttcggactgt 360catagcacct cttccagctg aaccttgtaa agagcttaac agttttttgc ttcttgtgac 420agagatgtaa ctg 433
<210>38
<211>383
<212>DNA
<213>辐射松(Pinus radiata)
<400>38cactggaggc aatggtcgga aactgatttg gcatggggtt cctcgaagca tcagagattg 60tcacaggaaa gttcatgaca gtagtgacgg actaattata caaagagatg tggcactctt 120tttctcaggt ggtgacataa atgaattgaa tcttagattg acaggacaca tattgaagga 180acaataatat atgcactttt caaagatcta tggactagga aaagtaagtc atatctcctg 240ttatttatct tctcctttgc tgctgattaa tattgtaaag gttcagatcc tttcagtagc 300aagctgtcat tgccagaaca acgagagaga aaaatcatat ctagaaagtg tataggttga 360ccacggcaca ggtgtatgcc att 383
<210>39
<211>1036
<212>DNA
<213>辐射松(Pinus radiata)
<400>39gctcatcatt ttgtgtgaaa ataagcgatg ggttggttcc acaggtgttt tggattcatt 60agaaagaaga agaagcagaa aagcccaaaa tctgagcctc cgtctcgtga acatttactg 120aagtccacac aagaagaatt cgagaataca aagggagctc aatacaaata tcaccgcaga 180tttcctgctg ttagggataa aaccgagcag gttgcgacgc gatcatttgg ggatttggat 240ggagcttcac taatagaaac tcctggcaga gaaaccctcc aaattgttat aacagagtgc 300ccaaatactc gtacagtatg ttctggatgt aaatccaggt taagcaactg gtgtccgtcc 360tgcagatgca accttggaaa ttttaggtgc ttagctcctg aaacggagac atcatctcaa 420gaacttactt gcatgtatca aagctatggt tgtgaggata tgtatcctta ctacagtgaa 480ttaagacatg aagctcagtg caattttagg ccatacaact gtccctatgc tggctccgaa 540tgcaagctag ttggagatat tccctttttg gtggctcatt taagagatga tcacaaagtt 600tatatgcata atagttgcac ctttgatcat cgatatgtaa agtcaaatcc actcgaggtt 660gagaatgcta tttggatgcc aactgtaatc aattgttttg ggcaattctt ttgtctacat 720tttgaagcgt ttctattaga catggcccct gtatatatag cttttctgat tttcatggga 780gatgataatg aagctaaaaa ctttagctat tgcctcgaga ctggaggcaa tggtcggaaa 840ctgatttggc atggggttcc tcgaagcatc agagattgtc acaggaaagt tcatgacagt 900agtgacggac taattataca aagagatgtg gcactctttt tctcaggtgg tgacataaat 960gaattgaatc ttagattgac aggacacata ttgaaggaac aataatatat gctggatcct 1020ctagagtcct gcttta 1036
<210>40
<211>563
<212>DNA
<213>辐射松(Pinus radiata)
<400>40ttttaaaggg cgcaatagtg atcaagtttc ggttgatctc caggttttta gatgccttgg 60tcagtatttt tgcctgcact tcgaggcctt tcagctcgga atggcaccag tctacatagc 120attccttcgg tttatgggtg atgacaacga ggcgaaaaac tacagttaca gcctcgaggt 180tggtgggaac gggagaaaga tgatctggca aggtgtgcct cggagcataa gggacagcca 240ccgcaaagtc cgcgatagtt tcgatggtct catcatccaa cgcaacatgg ctctcttctt 300ctctggtggc gaccggaagg aactgaagct tagggtgact ggtaggatct ggaaagaaca 360gtgacgacag ggtacttccg tctcatgctc ctttagatta tcctgctctt aaaaatcaga 420atagttcgtc cgcgtagtga tcaccacgtc ttcttgggca tgtatttttt gggaatttta 480gggggggcaa tgtctctttg cttgaactat gcatcgaata catgaatggg aagacagcaa 540aaatgtcgta tattccaagc aaa 563
<210>41
<211>868
<212>DNA
<213>辐射松(Pinus radiata)
<400>41ctctctctct ctctccctct acctccctcc ctccctcctt ctccctcatc cctctctcat 60ttttaagcct ccagatacaa atctttcatc tataaacata taaaagacgc gccttttcga 120acttttggcg ctccacccgc ccgttttctt cccttgattc tgctcggatc tgtcccctct 180gagccgatcc caacggtcaa aaccccgggt ttcgaagaaa aggtggacag gggtttctgt 240gttggattgt gtgtggagat ttggagcgac gagtcatggc ggatcaggcc ttggagggaa 300gccaaccggt tgatctgtcc aagcatcctt caggaatcgt tcccactctt cagaacatag 360tctcaacagt gaatttggac tgcaaattgg atcttaaggc cattgctttg caagctagaa 420atgctgagta taatcccaag cgttttgctg ctgtaattat gagaataagg gagccaaaga 480caacggcatt gatatttgct tcagggaaaa tggtttgtac tggagccaag agtgaacaac 540aatcaaagtt agcagcgcgg aagtatgctc gaatcattca gaaacttgga ttcccggcta 600aatttaagga tttcaaaatt caaaatattg tgggttcttg tgatgtgaaa ttccccatca 660ggcttgaagg tcttgcatat tcacatggtg ctttctcaag ttatgaacca gagctgttcc 720ctggattgat atatcggatg aaacagccaa aaatcgtgct gctaatcttt gtgtcaggaa 780aaattgtcct cactggggca aaggtgcgag atgagacgta cactgccttt gagaacatat 840accctgtgct cactgagttc aggaaaat 868
<210>42
<211>722
<212>DNA
<213>辐射松(Pinus radiata)
<400>42ttttcttcga gactcctctg ccgcagcagc tctctcctcg cccttttgag gatttacaat 60ccacatactc tataaaaacc gggacaaatc aaatcaactc aattcaaaca ccacctataa 120atacaaaagc taaccagatt caagagattc tctcaggatt tagtataaga aggatcgaga 180tttcattttc cgagggcatt ataaaagctt ttctgtttca ttcgatttcg attgtgtagt 240gaagagcatg gccgaacagg tcttggaagg gagtcagcca gtggatctcg agaagcatcc 300ttcaggcatc gttcccaccc tccagaatat agtgtccact gtaaacttgg attgcaaatt 360ggacttgaaa gccattgctc ttcaagctcg aaatgcagag tacaatccca agcgttttgc 420agcagtcata atgagaataa gggagcccaa aactacagca ctgatatttg catcagggaa 480gatggtttgc acaggtgcaa aaagtgaaca acagtcaaaa cttgctgcaa gaaagtatgc 540tcgtattatc caaaaattgg gctttcctgc tcatttcaag gattttaaga tccagaatat 600cgtggggtct tgtgatgtta aatttcctat tagattggaa gggcttgcat actcccatgg 660tgctttctca agctatgaac cagaattgtt tccaggccta atttatcgaa tgaaacagcc 720ca 722
<210>43
<211>884
<212>DNA
<213>辐射松(Pinus radiata)
<400>43aagggtttat ttgtcgctta gctgtgccct cgtaacagca gcgatcggtg tttatttgca 60tcttctgttg aatattggag ggctcctcac ggggctcgct tgcattggtt ctgtaatcgg 120gctcttatcc gtccctactt cctcgaacaa tgagggtaag agagctgcgc tgctcctggc 180agctgctgcg ttcaagggag ctactctggg accgctcatc gacgcggtca ttaatattga 240ctccagtata ctggtgagtg cgtttgttgg gacctctttg gccttcgctt gcttttcggc 300agcagcaatc acagccagga gacgggaata cctatttttg ggaggattat tgggctcggg 360aatcagcata ttgatgtggc tgcaactagc atcctcgatt tttggtggtt cttcggcgat 420ttacacattt gagatctact tcggtctgct agttttcctt gggtatatta tatttgacac 480acagatgatc atcgagaaag cggaccatgg agactatgat tatttaaaac attcactgga 540cctcttcatt gacttcgttg ctgtatttgt tcgcctgatg gtcataatgg caaagaatgc 600agacagtaaa tccagggaag ggaaaaagaa gagaagggct tgaactagtg atgtaatgag 660gcgtctttgg atacaaaaat agaagcactg gttcttgatg gcaatatggc cgtttaggtt 720gttttcagtt gtaaatacaa catctctctg aacattttgt tttgtttgga tatttcaaat 780agtggttccg taaaatcttg tagcgatgct ctttcttttt gtgtatggtg ttctatggac 840agatataaat ataaatagcc tttgtcatta aaaaaaaaaa aaaa 884
<210>44
<211>527
<212>DNA
<213>辐射松(Pinus radiata)
<400>44ccgatttcga agaacgagtt tggtcgacga tttcgcgatc cgcccgcctg cgaaaaaagt 60tcatcttcct cccaatcgat cgtttctcgc cgagcatcga atcgacatgg acgcgttcgc 120ttccctgttc cagtcgagcg gcaaggggtg gagccacgat tccctcaaga acttccgcca 180gatatctccc gccgtccaat ctcacctcaa gaatgtttat ctgtccttat gctgtgcctt 240gatggcttcg gccggtggtg cttacctgca tctgatgctg aacatcggcg ggctcctcac 300gacaattgct tgcatcggaa gcatcgtgtg gctgctttcg attcctccac atgaagagca 360aaagaggttt ggtctgctca tggcggcggc tctctttgaa ggagcgtgta tcggtcctct 420catcgaagcg gccattaagg tcgacccgag cattgtgata agcgcatttg tgggatctgc 480gctggccttc gcttgtttct cgggcgcagc atgttggcta ggcggag 527
<210>45
<211>864
<212>DNA
<213>辐射松(Pinus radiata)
<400>45aaagttacta gcaggaaatc caactaggta tcatgaagac taccaacgca ggctcgataa 60tgttggtgct cattattttt gggtgctgtt tcattggggt catagctaca tcttttgatt 120tctattactt cgttcaacag tggcctggtt catactgcga tactcgtaga ggatgctgtt 180accctcgcac gggaaggcct gcttccgaat tttccattca tggcctctgg cccaactaca 240agaccggtaa atggccacag ttctgtggtt cctccgaaga attcgactac tcaaagatct 300cagatctgga ggaggagctg aacaggtatt ggggttcgtt aagctgtcca agcagcgatg 360gacaggaatt ttggggacac gagtgggaga aacatggcac ttgctctctc aatcttgatg 420agcattcata ctttgagaag gctctctcct tgagacaaaa tatagacatt cttggggctc 480ttaaaactgc aggtattaaa cccgatggaa gccaatacag tttgagcgat atcaaggaag 540ccattaaaca aaacactggg cagctcccag gaatcgattg caacacgagc gcagagggag 600agcatcaact atatcaggtg tatgtgtgtg ttgataaatc cgatgcttcc actgttattg 660aatgccccat ttatccacac agcaattgcc catccatggt tgtgtttcct ccttttgggg 720aggatcagga ggaccgagat ggttacacag aaggaatgta cgagctgtag atctggacaa 780acagcatttc ttctctccgc atttgatttt tatcaatgaa atttccgatt ccaacatttt 840gtaaaaaaaa aaaaaaaaac tcga 864
<210>46
<211>199
<212>PRT
<213>辐射松(Pinus radiata)
<400>46Met Lys Met Ser Ser Phe Asn Tyr Pro Pro Gln Asp Asp Gly Glu Pro1 5 10 15Gln Tyr Ala Ser Ser Gly Ser Gly Glu Asn His Met Pro Asp Asp Asp
20 25 30Asp Pro Asn Trp Gly Asp Gly Tyr Lys Val Tyr Pro Gln Pro Asn Gln
35 40 45Gly Glu Ala Gln Asp Gln Pro Glu Tyr Ala Gly Phe Asn Glu Asp Tyr
50 55 60Gln Glu Gln Lys Asn Asp Ile Tyr Gly Lys Ile Met Leu Ser Gly Ile65 70 75 80Leu Val Phe Ile Phe Ile Ile Leu Leu Ala Ile Leu Leu His Val Tyr
85 90 95Ala Arg Trp Phe Trp Arg Arg Ser Ala Arg Phe Pro Asn Arg Asn Arg
100 105 110Arg Arg Ser Ser Ser Ile Arg His Arg Phe Asn Phe Ile Glu Glu Glu
115 120 125Pro Val Trp Leu Arg Asn Val Gly Leu Gln Ser Ala Val Leu Glu Thr
130 135 140Leu Pro Ile Phe Val Tyr Lys Ser Gln Asp Phe Thr Asp Gly Leu Glu145 150 155 160Cys Ala Val Cys Leu Cys Glu Phe Glu Glu Asn Glu Ile Ala Arg Leu
165 170 175Leu Pro Asn Cys Arg His Asn Phe His Val Glu Cys Ile Asp Met Trp
180 185 190Phe Arg Ser His Ser Thr Cys
195
<210>47
<211>116
<212>PRT
<213>辐射松(Pinus radiata)
<400>47Asn Ala Met Gly Asp Asp Tyr Gly Gly Gly Tyr Pro Asn Thr Lys Phe1 5 10 15Pro Asp Asp Gly Ser Ser Asn Ala Tyr Ala Leu Asn Gly Arg Ile Met
20 25 30Leu Ala Ala Ile Ile Val Leu Phe Phe Val Val Ile Ile Met Ile Ser
35 40 45Leu His Leu Tyr Ala Arg Trp Phe Leu Leu Arg Arg Gln Gln Arg Arg
50 55 60Arg Phe Leu Arg Arg Asn Arg Leu Asn Arg Arg Thr Gln Ile Val Phe65 70 75 80Tyr Ala Asp Phe Pro Ala Pro Gln Ala Ser Arg Gly Leu Asp Ser Ser
85 90 95Val Leu Lys Ser Leu Pro Val Phe Thr Phe Ser Ser Ser Ala Ala Ala
100 105 110Ala Ala Ala Ala
115
<210>48
<211>109
<212>PRT
<213>辐射松(Pinus radiata)
<400>48Asn Ala Met Gly Asp Asp Tyr Gly Phe Gly Asp Pro Asn Thr Glu Phe1 5 10 15Arg Gly Asn Gly Gln Ser Asn Ala Tyr Ala Leu Asn Gly Arg Ile Met
20 25 30Leu Ala Ala Ile Ile Val Leu Phe Phe Val Val Ile Ile Met Ile Ser
35 40 45Leu His Leu Tyr Ala Arg Trp Phe Leu Leu Arg Arg Gln Gln Arg Arg
50 55 60Arg Phe Leu Arg Arg Asn Arg Leu Asn Arg Arg Thr Gln Ile Val Phe65 70 75 80Tyr Ala Asp Phe Pro Ala Pro Gln Ala Ser Arg Gly Leu Asp Ser Ser
85 90 95Val Leu Lys Ser Leu Pro Val Phe Thr Phe Ser Ser Ser
100 105
<210>49
<211>104
<212>PRT
<213>辐射松(Pinus radiata)
<400>49Asn Lys Thr Val Ile Glu Ser Leu Pro Phe Phe Arg Phe Ser Ser Leu1 5 10 15Lys Gly Ser Lys Gln Gly Leu Glu Cys Ala Val Cys Leu Ser Lys Phe
20 25 30Glu Asp Ile Glu Ile Leu Arg Leu Leu Pro Lys Cys Arg His Ala Phe
35 40 45His Ile Asp Cys Ile Asp Tyr Trp Leu Glu Lys His Ser Ser Cys Pro
50 55 60Leu Cys Arg His Lys Val Ser Ala Glu Asp Pro Ala Asn Phe Thr Tyr65 70 75 80Thr Asn Ser Met Arg Leu Met Ser Gln Ser Asp Met Arg Gln Asp Ser
85 90 95Asn Leu Glu Leu Phe Val Gln Arg
100
<210>50
<211>129
<212>PRT
<213>辐射松(Pinus radiata)
<400>50Met Ser Ser Val Ser Glu Thr His Glu Pro Pro Gln Tyr Gly Ser Ala1 5 10 15Gln Gly Tyr Val Ile Ser Gly Lys Ile Met Leu Ser Ala Ile Ile Cys
20 25 30Leu Phe Val Val Val Leu Leu Met Phe Leu Leu His Leu Tyr Ala Arg
35 40 45Trp Ile Trp Arg His Ser Ala Arg Phe Ser Arg Arg Asn Arg Arg Arg
50 55 60Ser Ala Ser Arg Arg Arg Arg Leu Arg Phe Ser Gly Gln Val Pro Ala65 70 75 80Ser Leu Gln Asn Thr Gly Leu Asp Ser Ser Ile Leu Gln Thr Leu Pro
85 90 95Met Phe Val Tyr Lys Ser Gln Asp Phe Ile Asp Gly Leu Glu Cys Ala
100 105 110Val Cys Leu Cys Glu Leu Glu Glu Asn Glu Lys Ala Arg Leu Leu Pro
115 120 125Asn
<210>51
<211>115
<212>PRT
<213>辐射松(Pinus radiata)
<400>51Met Ala Arg Ser Ser Gly Asp Asp Ala Gln Ala Leu Phe His Ser Leu1 5 10 15Arg Ser Ala Tyr Ala Ala Thr Pro Lys Asn Leu Lys Ile Ile Asp Leu
20 25 30Tyr Val Ala Phe Ala Val Phe Thr Ala Leu Ile Gln Val Val Tyr Met
35 40 45Ala Leu Val Gly Ser Phe Pro Phe Asn Ser Phe Leu Ala Gly Gly Leu
50 55 60Ser Cys Ile Gly Thr Ala Val Leu Ala Val Cys Leu Arg Ile Gln Val65 70 75 80Asn Lys Glu Asn Lys Glu Phe Lys Asp Leu Pro Pro Glu Arg Ala Phe
85 90 95Ala Asp Phe Val Leu Cys Asn Leu Val Leu His Leu Val Ile Met Asn
100 105 110Phe Leu Gly
115
<210>52
<211>115
<212>PRT
<213>辐射松(Pinus radiata)
<400>52Met Ala Arg Ser Ser Gly Asp Asp Ala Gln Ala Leu Phe His Ser Leu1 5 10 15Arg Ser Ala Tyr Ala Ala Thr Pro Lys Asn Leu Lys Ile Ile Asp Leu
20 25 30Tyr Val Ala Phe Ala Val Phe Thr Ala Leu Ile Gln Val Val Tyr Met
35 40 45Ala Leu Val Gly Ser Phe Pro Phe Asn Ser Phe Leu Ala Gly Gly Leu
50 55 60Ser Cys Ile Gly Thr Ala Val Leu Ala Val Cys Leu Arg Ile Gln Val65 70 75 80Asn Lys Glu Asn Lys Glu Phe Lys Asp Leu Pro Pro Glu Arg Ala Phe
85 90 95Ala Asp Phe Val Leu Cys Asn Leu Val Leu His Leu Val Ile Met Asn
100 105 110Phe Leu Gly
115
<210>53
<211>115
<212>PRT
<213>辐射松(Pinus radiata)
<400>53Met Gly Thr Ser Thr Ala Lys Asp Ala Gln Val Leu Val Ala Ser Leu1 5 10 15Arg Ser Ala Tyr Ser Ala Thr Pro Thr Lys Leu Lys Ile Ile Asp Leu
20 25 30Tyr Val Val Tyr Ala Val Leu Thr Ala Val Val Gln Val Val Tyr Met
35 40 45Ala Ile Val Gly Ser Phe Pro Phe Asn Ala Phe Leu Ser Gly Val Leu
50 55 60Ser Cys Thr Gly Ser Ala Val Leu Ala Val Cys Leu Arg Met Gln Val65 70 75 80Asn Lys Glu Asn Lys Glu Phe Lys Asp Leu Pro Pro Glu Arg Ala Phe
85 90 95Ala Asp Phe Val Leu Cys Asn Leu Val Leu His Leu Val Ile Met Asn
100 105 110Phe Leu Gly
115
<210>54
<211>115
<212>PRT
<213>辐射松(Pinus radiata)
<400>54Met Gly Ser Ser Thr Ala Lys Asp Ala His Val Leu Val Ala Ser Leu1 5 10 15Arg Ser Ala Tyr Ser Ala Thr Pro Thr Lys Leu Lys Ile Ile Asp Leu
20 25 30Tyr Val Val Tyr Ala Ile Leu Thr Ala Val Val Gln Val Val Tyr Met
35 40 45Ala Ile Val Gly Ser Phe Pro Phe Asn Ala Phe Leu Ser Gly Val Leu
50 55 60Ser Cys Thr Gly Thr Ala Val Leu Ala Val Cys Leu Arg Met Gln Val65 70 75 80Asn Lys Glu Asn Lys Glu Phe Lys Asp Leu Pro Pro Glu Arg Ala Phe
85 90 95Ala Asp Phe Val Leu Cys Asn Leu Val Leu His Leu Val Ile Met Asn
100 105 110Phe Leu Gly
115
<210>55
<211>152
<212>PRT
<213>辐射松(Pinus radiata)
<400>55Glu Ala Leu Met Phe Leu Ser His Phe Ile Gly Asp Ile His Gln Pro1 5 10 15Leu His Val Gly Phe Thr Thr Asp Arg Gly Ala Asn Glu Ile Glu Val
20 25 30Arg Trp Tyr Thr Arg Lys Gln Asn Leu His His Val Trp Asp Ser Asn
35 40 45Ile Ile Glu Thr Ala Glu Glu Arg Tyr Tyr Ser Ser Asp Thr Asp Gly
50 55 60Leu Val Asp Ala Ile Gln Gln Asn Ile Thr Asn Asp Trp Ala Glu Glu65 70 75 80Val Lys Gly Trp Glu Thr Cys Ser Ser Thr Lys Pro Pro Cys Pro Asp
85 90 95Ile Tyr Ala Ser Glu Ser Ile Ala Ala Ala Cys Gln Trp Ala Tyr Lys
100 105 110Gly Val Ser Glu Gly Ser Val Leu Glu Asp Pro Tyr Phe Leu Ser Arg
115 120 125Leu Pro Thr Val Asn Leu Arg Leu Ala Lys Gly Gly Val Arg Leu Ala
130 135 140Ala Thr Leu Asn Arg Ile Phe Met145 150
<210>56
<211>161
<212>PRT
<213>辐射松(Pinus radiata)
<400>56Leu Pro Val Val Gly Asp Gln Lys Trp Val Ile Trp Ile Cys Ser Phe1 5 10 15Asn Val Pro Met Ala Pro Gly Lys Thr Arg Ser Ile Val Cys Ser Ala
20 25 30Arg Asn Phe Phe Gln Phe Thr Met Pro Gly Pro Ala Trp Trp Gln Val
35 40 45Ile Pro Arg Trp His Glu His Trp Thr Ser Asn Lys Val Tyr Asp Gly
50 55 60Asp Met Ile Val Leu Gln Gly Gln Glu Lys Ile Phe Leu Ser Lys Ser65 70 75 80Met Glu Gly Gln Glu Asp Val Asn Glu Gln Tyr Thr Lys Ile Thr Phe
85 90 95Thr Pro Thr Gln Ala Asp Arg Phe Val Leu Ala Phe Arg Asn Trp Leu
100 105 110Arg Arg His Gly Asn Ser Gln Pro Glu Trp Phe Gly Ser Ser Ser Gln
115 120 125Lys Pro Leu Pro Ser Thr Val Leu Ser Lys Arg Gln Met Leu Asp Arg
130 135 140Phe Glu Gln His Thr Leu Lys Cys Ser Ser Cys Arg Lys Ala Tyr Glu145 150 155 160Ala
<210>57
<211>256
<212>PRT
<213>辐射松(Pinus radiata)
<400>57Ile Gln Met Asp Ala Leu Thr His Gly Thr Ser Val Gly Phe Ile Thr1 5 10 15Phe Ser Pro Lys Ile Gly Ser Gln Cys Asn Asn Lys Ser Lys Gly Asp
20 25 30Cys Asn Phe Ser Phe Leu Thr Ala Lys Glu Gln Ser Ile Arg Arg Arg
35 40 45Arg Asn Asn Phe Ala Thr Arg Arg Arg Asp Leu His Val Val Ser Ala
50 55 60Thr Val Ala Pro Pro Thr Ile Pro Gly Ser Ser Ser Ala Glu Asp Phe65 70 75 80Asp Lys Asp Arg Glu Ala Glu Glu Glu Ser Gly Lys Phe Ile Trp Arg
85 90 95Asp His Trp Tyr Pro Val Ser Leu Ile Glu Asp Leu Asp Pro Lys Ile
100 105 110Pro Thr Pro Phe Gln Leu Leu Gly Arg Glu Ile Val Leu Trp Gln Asp
115 120 125Ala Glu Gly Asn Trp Lys Ala Phe Glu Asp Lys Cys Pro His Arg Leu
130 135 140Ala Pro Leu Ser Glu Gly Arg Leu Asp Glu Asn Gly Trp Leu Gln Cys145 150 155 160Ser Tyr His Gly Trp Ser Phe Lys Ala Asp Gly Ser Cys Ala Arg Ile
165 170 175Pro Gln Ala Ala Ser Glu Gly Pro Glu Ser Arg Ala Ala Arg Ser Pro
180 185 190Arg Ala Cys Ala Val Ser Phe Pro Thr Thr Val Ser Gln Gly Leu Leu
195 200 205Phe Val Trp Pro Asp Glu Asn Gly Trp Glu Arg Ala Ser Lys Phe Glu
210 215 220Pro Pro Leu Leu Pro Asp Ala Phe Ser Asp Pro Arg Phe Ser Thr Val225 230 235 240Thr Ile Gln Arg Asp Leu Phe Tyr Gly Tyr Asp Thr Leu Met Glu Asn
245 250 255
<210>58
<211>129
<212>PRT
<213>辐射松(Pinus radiata)
<400>58Met Leu Ser Gly Gly Tyr Ala Thr Arg Ser Asp Thr Thr Thr Val Asn1 5 10 15Asn Gly Ser Ala Asn Gly Pro Ile Gly Ser Ala Pro Pro Arg Ile Asn
20 25 30Ser Ile Gln Asn Asn Asn Pro Gly Ala Val Arg Pro Gly Trp Gly Thr
35 40 45Met Pro Leu His Met Asn Pro Tyr His Pro Gln Ser Met Pro Leu Pro
50 55 60Pro Pro Asn Gly Met Gln Gly Gln Leu Val Cys Ser Gly Cys Arg Thr65 70 75 80Leu Leu Val Tyr Pro Gln Gly Ala Pro Asn Val Cys Cys Ala Val Cys
85 90 95Asn Thr Val Thr Pro Val Pro Pro Pro Gly Thr Glu Met Ala Gln Leu
100 105 110Ile Cys Gly Arg Cys Arg Thr Leu Leu Met Tyr Val Arg Gly Ala Thr
115 120 125Ser
<210>59
<211>56
<212>PRT
<213>辐射松(Pinus radiata)
<400>59Ser Val Pro Pro Gln Arg Ile Asp Gly Pro Pro Ser Gly Thr Thr Pro1 5 10 15Ser Thr Ser Thr Ser Met Pro Gln Ser Thr Gln Thr Val Val Val Glu
20 25 30Asn Pro Met Ser Val Asp Glu Ser Gly Lys Leu Val Thr Asn Val Val
35 40 45Val Gly Val Thr Thr Glu Lys Arg
50 55
<210>60
<211>338
<212>PRT
<213>辐射松(Pinus radiata)
<400>60Thr Thr Arg Asn Asn Val Ser Pro Ala Thr Ser Thr Ser Ser Ser Thr1 5 10 15Ala Phe Thr Ile Gln Gly Asn Val Tyr Pro Asp Gly Leu Tyr Tyr Val
20 25 30Ser Met Leu Ile Gly Asn Pro Pro Lys Pro Tyr His Leu Asp Val Asp
35 40 45Thr Gly Ser Asp Leu Thr Trp Ile Gln Cys Asp Ala Pro Cys Arg Ser
50 55 60Cys Ala Lys Gly Pro His Ala Leu Tyr Lys Pro Lys Lys Ser Gln Leu65 70 75 80Val Ser Cys Met Ala Pro Met Cys Val Asn Val Gln Ala Gly His Ser
85 90 95His Glu Cys Thr Ser Thr Ser Glu Gln Cys Asp Tyr Glu Ile Glu Tyr
100 105 110Ala Asp Leu Gly Ser Ser Met Gly Val Leu Ala Arg Asp Asn Ile Arg
115 120 125Val Leu Leu Thr Asn Gly Ser Ile Ala Arg Thr Asn Phe Val Phe Gly
130 135 140Cys Ala Tyr Asp Gln Gln Gly Ser Leu Ala Val Ser Pro Ala Val Thr145 150 155 160Asp Gly Val Leu Gly Leu Ser Ser Ala Gln Val Ser Leu Pro Ser Gln
165 170 175Leu Ala Ser Gln Gly Leu Thr Lys Asn Val Ile Gly His Cys Ile Ala
180 185 190Gly Asp Glu Arg Asp Glu Arg Ser Gly Gly Tyr Met Phe Phe Gly Asn
195 200 205Asp Leu Val Pro Val Trp Gly Met Thr Trp Val Pro Met Phe Gly Lys
210 215 220Pro Ala Met Lys Leu Tyr Ser Val Gly Ser Ala Asn Met Lys Leu Gly225 230 235 240Asn Arg Pro Leu Val Ser Asn Asp Leu Lys Asn Lys Leu Gly Gly Val
245 250 255Val Phe Asp Ser Gly Ser Ser Phe Thr Tyr Leu Thr Gln Thr Ala Tyr
260 265 270Phe Ala Phe Val Ser Ala Val Lys Glu Asn Leu Phe Gly Gly Gly Leu
275 280 285Val Gln Asp Leu Ser Asp Lys Thr Leu Pro Leu Cys Trp Arg Ala Arg
290 295 300His Pro Ile Arg Ser Ile Ala Asp Val Lys Pro Phe Phe Lys Pro Leu305 310 315 320Thr Leu Asp Phe Gly Gly Asn Pro Trp Leu Ile Lys Thr Lys Gln Phe
325 330 335Asp Ile
<210>61
<211>83
<212>PRT
<213>辐射松(Pinus radiata)
<400>61Arg His Glu Ile Lys Thr Lys Gln Phe Asp Ile Pro Pro Glu Gly Tyr1 5 10 15Leu Val Ile Ser Ser His Gly Asn Val Cys Leu Gly Ile Leu Asn Gly
20 25 30Ser Glu Val Asn Asp Gly Ala Thr Asn Ile Ile Gly Asp Ile Ser Leu
35 40 45Gln Gly His Leu Ile Val Tyr Asp Asn Val Lys Asn Gln Ile Gly Trp
50 55 60Val His Ala Asp Cys His Lys Pro Pro Lys Met Met Lys Ala Phe Pro65 70 75 80Phe Phe Lys
<210>62
<211>316
<212>PRT
<213>辐射松(Pinus radiata)
<400>62Met Ser Thr Leu Thr Val Pro Gln Pro Leu Pro Pro Val Ala Asp Asp1 5 10 15Cys Glu Gln Leu Arg Thr Ala Phe Ala Gly Trp Gly Thr Asn Glu Lys
20 25 30Leu Ile Ile Ser Ile Leu Gly His Arg Asn Ala Ala Gln Arg Lys Leu
35 40 45Ile Arg Gln Thr Tyr Ala Glu Thr Tyr Gly Glu Asp Leu Leu Lys Ala
50 55 60Leu Asp Arg Glu Leu Thr Asn Asp Phe Glu Arg Leu Val Val Leu Trp65 70 75 80Ser Leu Asp Pro Ala Glu Arg Asp Ala Tyr Leu Ala Asn Glu Ala Thr
85 90 95Lys Arg Trp Thr Ser Ser Asn Gln Val Leu Met Glu Ile Ala Cys Thr
100 105 110Arg Ser Pro Gln Gln Leu Leu Met Ala Arg Gln Ala Tyr His Ala Arg
115 120 125Tyr Lys Lys Ser Met Glu Glu Asp Val Ala His His Thr Thr Gly Asp
130 135 140Phe Arg Lys Leu Leu Val Pro Leu Gly Ser Ser Tyr Arg Asn Asp Gly145 150 155 160Asp Glu Val Asn Met Thr Leu Ala Lys Ala Glu Ala Lys Ile Leu His
165 170 175Glu Lys Ile Ser Glu Lys Ala Tyr Gly His Glu Asp Leu Ile Arg Ile
180 185 190Leu Ala Thr Arg Ser Lys Ala Gln Val Asn Ala Thr Leu Asn His Tyr
195 200 205Lys Asn Glu Phe Gly Asn Asp Ile Asn Lys Asp Leu Lys Thr Asp Pro
210 215 220Lys Asp Ala Phe Leu Thr Ile Leu Arg Ala Thr Val Lys Cys Leu Thr225 230 235 240Arg Pro Glu Lys Tyr Phe Glu Lys Val Leu Arg Leu Ala Ile Asn Lys
245 250 255Arg Gly Thr Asp Glu Gly Ala Leu Thr Arg Val Val Ala Thr Arg Ala
260 265 270Glu Val Asp Met Lys Phe Ile Ser Glu Glu Tyr Gln Arg Arg Asn Ser
275 280 285Ile Pro Leu Asp Arg Ala Ile Val Lys Asp Thr Thr Gly Asp Tyr Glu
290 295 300Lys Met Leu Leu Ala Leu Ile Gly His Val Glu Ala305 310 315
<210>63
<211>111
<212>PRT
<213>辐射松(Pinus radiata)
<400>63Ser Ile Phe Ile Pro Tyr Arg Gln Arg Leu Val Val Leu Trp Ser Leu1 5 10 15Asp Pro Ala Glu Arg Asp Ala Tyr Leu Ala Asn Glu Ala Thr Lys Arg
20 25 30Trp Thr Ser Ser Asn Gln Val Leu Met Glu Ile Ala Cys Thr Arg Ser
35 40 45Pro Gln Gln Leu Leu Met Ala Arg Gln Ala Tyr His Ala Arg Tyr Lys
50 55 60Lys Ser Leu Glu Glu Asp Val Ala His His Thr Thr Gly Asp Phe Arg65 70 75 80Lys Leu Leu Val Pro Leu Val Ser Ser Tyr His Tyr Asp Gly Asp Glu
85 90 95Val Asn Met Thr Leu Ala Lys Ala Glu Ala Lys Ile Leu His Glu
100 105 110
<210>64
<211>73
<212>PRT
<213>辐射松(Pinus radiata)
<400>64Tyr Leu Ala Asn Glu Ala Thr Lys Arg Trp Thr Ser Ser Asn Gln Val1 5 10 15Leu Met Glu Ile Ala Cys Thr Arg Ser Pro Gln Gln Leu Leu Met Ala
20 25 30Arg Gln Ala Tyr His Ala Arg Tyr Lys Lys Ser Leu Glu Glu Asp Val
35 40 45Gly His His Thr Thr Gly Asp Phe Arg Lys Leu Leu Val Pro Leu Val
50 55 60Ser Ser Tyr Arg Tyr Asp Gly Asp Glu65 70
<210>65
<211>239
<212>PRT
<213>辐射松(Pinus radiata)
<400>65Met Ala Thr Ile Ala Val Pro Pro Ser Val Pro Ser Pro Ala Glu Asp1 5 10 15Ala Glu Gln Leu Gln Lys Ala Phe Ala Gly Trp Gly Thr Asn Glu Asp
20 25 30Leu Ile Ile Ser Ile Leu Pro His Arg Asn Ala Ala Gln Arg Lys Val
35 40 45Ile Arg Gln Thr Tyr Ala Glu Thr Tyr Gly Glu Asp Leu Leu Lys Ala
50 55 60Leu Asp Lys Glu Leu Ser Ser Asp Phe Glu Arg Ser Val Leu Leu Trp65 70 75 80Thr Leu Asp Pro Ala Glu Arg Asp Ala Phe Leu Ser Asn Glu Ala Thr
85 90 95Lys Arg Leu Thr Ser Ser Asn Trp Val Leu Met Glu Ile Ala Cys Thr
100 105 110Arg Ser Ser Met Glu Leu Phe Met Val Arg Gln Ala Tyr His Ala Arg
115 120 125Tyr Lys Lys Ser Leu Glu Glu Asp Ile Ala Tyr His Thr Thr Gly Asp
130 135 140Phe Arg Lys Leu Leu Val Pro Leu Ala Ser Thr Phe Arg Tyr Glu Gly145 150 155 160Pro Glu Val Asn Met Thr Leu Ala Arg Ser Glu Ala Lys Ile Leu His
165 170 175Glu Lys Ile His Glu Lys Ala Tyr Asn His Asp Glu Leu Ile Arg Ile
180 185 190Val Thr Thr Arg Ser Lys Ala Gln Leu Asn Ala Thr Leu Asn Tyr Tyr
195 200 205Asn Asn Glu Phe Gly Asn Ala Ile Asn Lys Asp Leu Lys Ala Asp Pro
210 215 220Asn Asp Glu Phe Leu Lys Leu Leu Arg Ser Ala Ile Lys Cys Leu225 230 235
<210>66
<211>184
<212>PRT
<213>辐射松(Pinus radiata)
<400>66Met Ser Thr Ile Ile Val Pro Val Pro Ile Pro Thr Pro Ser Glu Asp1 5 10 15Ser Glu Arg Leu Arg Lys Ala Phe Glu Gly Trp Gly Thr Asn Glu Lys
20 25 30Ser Ile Ile Gln Ile Leu Gly His Arg Thr Ala Ala Gln Arg Lys Val
35 40 45Ile Arg Gln Ser Tyr Phe Gln Leu Tyr Glu Glu Asp Leu Leu Lys Arg
50 55 60Leu Glu Ser Glu Leu Ser Ser Asp Phe Glu Lys Ala Val Phe Leu Trp65 70 75 80Val Leu Asp Pro Ala Glu Arg Asp Ala Val Ile Ser His Gly Ala Ile
85 90 95Lys Lys Trp Asn Ala Lys Asn Ile Ser Leu Leu Glu Ile Ser Ser Ala
100 105 110Arg Ser Ser Ala Glu Leu Leu Met Val Arg Gln Ala Tyr His Ile Arg
115 120 125Tyr Lys Lys Ser Leu Glu Glu Asp Val Ala Ala His Thr Ser Gly Asn
130 135 140Phe Arg Lys Leu Leu Val Ala Leu Val Ser Ser Tyr Arg Tyr Glu Gly145 150 155 160Pro Glu Val Asp Met His Leu Ala Ser Tyr Glu Ala Lys Lys Leu Ser
165 170 175Glu Ser Ile Thr Glu Gln Lys Arg
180
<210>67
<211>275
<212>PRT
<213>辐射松(Pinus radiata)
<400>67Met Ser Val Gln Arg Ala Leu Ser Asn Ile Ala Ala Leu Ala Ile Ser1 5 10 15Val Gly Thr Gly Val Gly Leu Leu Asn Ala Ser Leu Tyr Thr Val Asp
20 25 30Gly Gly His Lys Ala Val Leu Phe Asp Arg Phe Arg Gly Val Leu Asp
35 40 45Thr Thr Val Gly Glu Gly Thr His Phe Leu Ile Pro Trp Leu Gln Lys
50 55 60Pro Tyr Ile Phe Glu Ile Arg Thr Lys Pro Arg Ser Ile Ser Thr Ile65 70 75 80Thr Gly Thr Lys Asp Leu Gln Met Val Asn Ile Ser Leu Arg Ile Leu
85 90 95Ala Arg Pro Lys Glu Asp Ser Leu Pro Asp Ile Phe Gln Arg Leu Gly
100 105 110Leu Asp Tyr Asp Glu Arg Val Leu Pro Ser Ile Gly Asn Glu Val Leu
115 120 125Lys Ala Val Val Ala Gln Phe Asn Ala Asp Gln Leu Leu Thr Glu Arg
130 135 140Pro Thr Val Ser Ala Leu Val Arg Glu Ala Leu Leu His Arg Ala Lys145 150 155 160Asp Phe Asn Ile Leu Leu Asp Asp Val Ala Ile Thr His Leu Ser Tyr
165 170 175Gly Pro Glu Phe Ser Lys Ala Val Glu Gln Lys Gln Val Ala Gln Gln
180 185 190Glu Ala Glu Arg Ser Lys Phe Val Val Ala Lys Ala Glu Gln Glu Arg
195 200 205Arg Ala Ala Val Val Arg Ala Glu Gly Glu Ser Glu Ala Ala Lys Leu
210 215 220Ile Ser Leu Ala Thr Ser Ala Ala Gly Leu Gly Leu Ile Glu Leu Arg225 230 235 240Arg Ile Glu Ala Ala Lys Glu Ile Ala Ser Thr Leu Ser Arg Ser Pro
245 250 255Asn Val Val Tyr Leu Pro Ser Gly Asn Asn Met Leu Leu Gly Leu Asn
260 265 270Pro Ser His
275
<210>68
<211>146
<212>PRT
<213>辐射松(Pinus radiata)
<400>68Met Gly Ser Ser Gln Ala Ala Val Ser Phe Leu Thr Asn Val Ala Arg1 5 10 15Ala Ala Phe Gly Leu Gly Ala Ala Gly Thr Ala Leu Asn Ala Ser Leu
20 25 30Tyr Thr Val Asp Gly Gly Gln Arg Ala Val Ile Phe Asp Arg Leu Arg
35 40 45Gly Val Met Asp Glu Thr Val Gly Glu Gly Thr His Phe Leu Val Pro
50 55 60Trp Leu Gln Lys Pro Phe Ile Phe Asp Ile Arg Thr Arg Pro His Thr65 70 75 80Phe Ser Ser Val Ser Gly Thr Lys Asp Leu Gln Met Val Asn Leu Thr
85 90 95Leu Arg Val Leu Ser Arg Pro Gln Val Ser Arg Leu Pro Tyr Ile Phe
100 105 110Arg His Leu Gly Leu Glu Tyr Asp Glu Lys Val Leu Pro Ser Ile Gly
115 120 125Asn Glu Val Leu Lys Ala Val Val Ala Gln Phe Asn Ala Asp Ser Ala
130 135 140Ser Tyr145
<210>69
<211>168
<212>PRT
<213>辐射松(Pinus radiata)
<400>69Val Leu Pro Ser Ile Ile His Glu Thr Leu Lys Ala Val Val Ala Gln1 5 10 15Tyr Asn Ala Ser Gln Leu Ile Thr Gln Arg Glu Ala Val Ser Arg Glu
20 25 30Ile Arg Arg Ile Leu Thr Glu Arg Ala Ala Asn Phe Tyr Ile Ala Leu
35 40 45Asp Asp Val Ser Ile Thr Ser Leu Thr Phe Gly Arg Glu Phe Thr Ala
50 55 60Ala Ile Glu Ala Lys Gln Val Ala Ala Gln Glu Ala Glu Arg Ala Lys65 70 75 80Phe Val Val Glu Lys Ala Glu Gln Asp Lys Lys Ser Ala Ile Ile Arg
85 90 95Ala Gln Gly Glu Ala Thr Ser Ala Gln Leu Ile Gly Glu Ala Ile Ser
100 105 110Asn Asn Pro Ala Phe Ile Thr Leu Arg Lys Ile Glu Ala Ser Arg Glu
115 120 125Ile Ala His Thr Leu Ser Asn Ser Thr Asn Arg Ile Phe Leu Ser Ser
130 135 140Asp Ser Leu Leu Leu Asn Leu Gln Asp Met Ser Leu Asp Asp Ala His145 150 155 160Lys Pro Pro Pro Lys Pro Lys Lys
165
<210>70
<211>119
<212>PRT
<213>辐射松(Pinus radiata)
<400>70Met Asn Phe Asn Asn Val Arg Val Pro Gly Gly Gly Gly Ala Ala Trp1 5 10 15Ala Leu Thr Lys Ala Val Val Leu Gly Gly Ala Gly Leu Tyr Gly Ala
20 25 30Leu Asn Ser Leu Tyr Asn Val Glu Gly Gly His Arg Ala Ile Val Phe
35 40 45Asn Arg Ile Val Gly Val Lys Asp Lys Val Tyr Pro Glu Gly Thr His
50 55 60Leu Met Ile Pro Trp Phe Asp Arg Pro Val Ile Tyr Asp Val Arg Ala65 70 75 80Arg Pro His Leu Val Glu Ser Thr Ser Gly Ser Arg Asp Leu Gln Met
85 90 95Val Lys Ile Gly Leu Arg Val Leu Thr Arg Pro Met Pro Asp Gln Leu
100 105 110Pro Thr Ile Tyr Arg Pro Leu
115
<210>71
<211>86
<212>PRT
<213>辐射松(Pinus radiata)
<400>71Met Asp Phe Arg Asn Val Lys Val Pro Lys Val Pro Gly Gly Gly Ala1 5 10 15Thr Ser Ala Leu Leu Lys Leu Gly Val Ile Gly Gly Ile Ala Leu Tyr
20 25 30Ala Ala Thr Asn Ser Leu Tyr Asn Val Glu Gly Gly His Arg Ala Ile
35 40 45Val Phe Asn Arg Leu Val Gly Val Lys Asp Lys Val Tyr Pro Glu Gly
50 55 60Thr His Ile Met Ile Pro Trp Phe Glu Arg Pro Val Ile Tyr Asp Val65 70 75 80Arg Ala Arg Pro His Leu
85
<210>72
<211>186
<212>PRT
<213>辐射松(Pinus radiata)
<400>72Met Arg Arg Phe Phe Cys Cys Thr Cys Ser Thr Glu Gly Ala Pro Glu1 5 10 15Gln Pro Glu Ala His Phe Leu Asn Ala Ala Lys Asn Asn Gly Asn Gly
20 25 30Phe Gln Gly Asp Tyr Lys Met Ser Glu Gly Ala Lys Ser Gly Pro Pro
35 40 45Gln Lys Ile Ala Pro Ile Glu Ala Pro Ala Leu Ser Leu Glu Glu Leu
50 55 60Lys Glu Ala Thr Asp Asn Phe Gly Ala Lys Ala Leu Ile Gly Glu Gly65 70 75 80Ser Tyr Gly Arg Val Tyr Tyr Ala Met Leu Ser Asp Gly Gln Pro Ala
85 90 95Ala Ile Lys Lys Leu Asp Val Asn Ser Gln Pro Glu Ala Asn Ser Glu
100 105 110Phe Leu Ala Gln Ile Ser Met Val Ser Arg Leu Lys His Asp His Ile
115 120 125Val Glu Leu Val Gly Tyr Cys Val Glu Gly Thr Leu Arg Val Leu Ala
130 135 140Tyr Glu Phe Ala Thr Met Gly Ser Leu His Asp Ile Leu His Gly Arg145 150 155 160Lys Gly Val Gln Gly Ala Gln Pro Gly Pro Val Leu Asp Trp Met Gln
165 170 175Arg Val Lys Ile Ala Val Gly Ala Ala Lys
180 185
<210>73
<211>196
<212>PRT
<213>辐射松(Pinus radiata)
<400>73Met Ser Ile Cys Arg Phe Ile Lys Cys Val Thr Val Gly Asp Gly Ala1 5 10 15Val Gly Lys Thr Cys Leu Leu Ile Ser Tyr Thr Ser Asn Thr Phe Pro
20 25 30Thr Asp Tyr Val Pro Thr Val Phe Asp Asn Phe Ser Ala Asn Val Val
35 40 45Val Asp Gly Asn Asn Val Asn Leu Gly Leu Trp Asp Thr Ala Gly Gln
50 55 60Glu Asp Tyr Asn Arg Leu Arg Pro Leu Ser Tyr Arg Gly Thr Asp Val65 70 75 80Phe Leu Leu Ala Phe Ser Leu Ile Ser Lys Ala Ser Tyr Glu Asn Val
85 90 95Ser Lys Lys Trp Ile Pro Glu Leu Lys His Tyr Val Pro Ser Val Pro
100 105 110Ile Val Leu Val Gly Thr Lys Leu Asp Leu Arg Asp Asp Lys Gln Phe
115 120 125Phe Ser Asp His Pro Gly Ala Ala Pro Ile Thr Thr Ala Gln Gly Glu
130 135 140Glu Leu Lys Asn Gln Ile Gly Ala Val Ala Tyr Ile Glu Cys Ser Ser145 150 155 160Lys Thr Gln Gln Asn Val Lys Ala Val Phe Asp Ala Ala Ile Asn Ser
165 170 175Val Leu Gln Leu Pro Lys Pro Val Lys Pro Arg Lys Lys Arg Gln Thr
180 185 190Cys Ala Val Leu
195
<210>74
<211>115
<212>PRT
<213>辐射松(Pinus radiata)
<400>74Met Ala Ala Ser Ala Ser Arg Phe Ile Lys Cys Val Thr Val Gly Asp1 5 10 15Gly Ala Val Gly Lys Thr Cys Met Leu Ile Cys Tyr Thr Ser Asn Lys
20 25 30Phe Pro Thr Asp Tyr Ile Pro Thr Val Phe Asp Asn Phe Ser Ala Asn
35 40 45Val Val Val Glu Gly Thr Thr Val Asn Leu Gly Leu Trp Asp Thr Ala
50 55 60Gly Gln Glu Asp Tyr Asn Arg Leu Arg Pro Leu Ser Tyr Arg Gly Ala65 70 75 80Asp Val Phe Val Leu Ala Phe Ser Leu Val Ser Arg Ala Ser Tyr Glu
85 90 95Asn Ile Leu Lys Lys Trp Ile Pro Glu Leu Gln His Tyr Ala Pro Gly
100 105 110Asn Pro Ser
115
<210>75
<211>94
<212>PRT
<213>辐射松(Pinus radiata)
<400>75Met Ser Ala Ser Arg Phe Ile Lys Cys Val Thr Val Gly Asp Gly Ala1 5 10 15Val Gly Lys Thr Cys Met Leu Ile Ser Tyr Thr Ser Asn Thr Phe Pro
20 25 30Thr Asp Tyr Val Pro Thr Val Phe Asp Asn Phe Ser Ala Asn Val Val
35 40 45Val Asp Gly Ser Thr Val Asn Leu Gly Leu Trp Asp Thr Ala Gly Gln
50 55 60Glu Asp Tyr Asn Arg Leu Arg Pro Leu Ser Tyr Arg Gly Ala Asp Val65 70 75 80Phe Leu Leu Thr Phe Ser Leu Ile Ser Lys Ala Ser Tyr Glu
85 90
<210>76
<211>136
<212>PRT
<213>辐射松(Pinus radiata)
<400>76Ile Lys Cys Val Thr Val Gly Asp Gly Ala Val Gly Lys Thr Cys Leu1 5 10 15Leu Ile Ser Tyr Thr Ser Asn Thr Phe Pro Thr Asp Tyr Val Pro Thr
20 25 30Val Phe Asp Asn Phe Ser Ala Asn Val Val Val Asn Gly Ser Thr Val
35 40 45Asn Leu Gly Leu Trp Asp Thr Ala Gly Gln Glu Asp Tyr Asn Arg Leu
50 55 60Arg Pro Leu Ser Tyr Arg Gly Ala Asp Val Phe Ile Leu Ala Phe Ser65 70 75 80Leu Ile Ser Lys Ala Ser Tyr Glu Asn Val Ser Lys Lys Trp Ile Pro
85 90 95Glu Leu Lys His Tyr Ala Pro Gly Val Pro Ile Ile Leu Val Gly Thr
100 105 110Lys Leu Asp Leu Arg Asp Asp Lys Gln Phe Phe Ile Asp His Pro Gly
115 120 125Ala Val Pro Ile Thr Thr Gln Gln
130 135
<210>77
<211>110
<212>PRT
<213>辐射松(Pinus radiata)
<400>77Lys Cys Val Thr Val Gly Asp Gly Ala Val Gly Lys Thr Cys Leu Leu1 5 10 15Ile Ser Tyr Thr Ser Asn Thr Phe Pro Thr Asp Tyr Val Pro Thr Val
20 25 30Phe Asp Asn Phe Ser Ala Asn Val Val Val Asn Gly Ser Thr Val Asn
35 40 45Leu Gly Leu Trp Asp Thr Ala Gly Gln Glu Asp Tyr Asn Arg Leu Arg
50 55 60Pro Leu Ser Tyr Arg Gly Ala Asp Val Phe Ile Leu Ala Phe Ser Leu65 70 75 80Ile Ser Lys Ala Ser Tyr Glu Asn Val Ser Lys Lys Trp Ile Pro Val
85 90 95Leu Lys His Tyr Ala Pro Gly Val Pro Ile Val Leu Val Gly
100 105 110
<210>78
<211>146
<212>PRT
<213>辐射松(Pinus radiata)
<400>78Met Ser Thr Ala Arg Phe Ile Lys Cys Val Thr Val Gly Asp Gly Ala1 5 10 15Val Gly Lys Thr Cys Met Leu Ile Ser Tyr Thr Ser Asn Thr Phe Pro
20 25 30Thr Asp Tyr Val Pro Thr Val Phe Asp Asn Phe Ser Ala Asn Val Val
35 40 45Val Asp Gly Ser Thr Val Asn Leu Gly Leu Trp Asp Thr Ala Gly Gln
50 55 60Glu Asp Tyr Asn Arg Leu Arg Pro Leu Ser Tyr Arg Gly Ala Asp Val65 70 75 80Phe Leu Leu Ala Phe Ser Leu Ile Ser Lys Ala Ser Tyr Glu Asn Ile
85 90 95Phe Lys Asn Trp Ile Pro Glu Leu Arg His Tyr Ala Pro Ser Val Pro
100 105 110Ile Ile Leu Val Gly Thr Lys Leu Asp Leu Arg Glu Asp Lys Gln Phe
115 120 125Phe Ala Asp His Pro Gly Ala Ala Pro Ile Ser Thr Ala Gln Gly Glu
130 135 140Asn Leu145
<210>79
<211>54
<212>PRT
<213>辐射松(Pinus radiata)
<400>79Met Ser Ala Ser Lys Phe Ile Lys Cys Val Thr Val Gly Asp Gly Ala1 5 10 15Val Gly Lys Thr Cys Met Leu Ile Cys Tyr Thr Ser Asn Lys Phe Pro
20 25 30Thr Asp Tyr Ile Pro Thr Val Phe Asp Asn Phe Ser Ala Asn Val Ala
35 40 45Val Asp Gly Asn Ile Val
50
<210>80
<211>182
<212>PRT
<213>辐射松(Pinus radiata)
<400>80Glu Leu Lys Asp Ile Asp Thr Asp Thr Phe Ser Tyr Phe Glu Gly Leu1 5 10 15Met Glu Glu Ser Ser Leu Val Ser Ser Ile Gln Ile Leu Glu Lys Asp
20 25 30Tyr Glu Asp Ala Ile Tyr Asp Arg Gly Glu Leu Asp Glu Arg Met Phe
35 40 45Val Asn Glu Glu Asp Ser Leu Phe Gly Ser Ala Ser Asn Ser Gly Gly
50 55 60Sar Val Thr Val Ser Gly Val Lys Arg Lys Phe Asp Ser Ile Ser Ser65 70 75 80Pro Thr Lys Thr Ile Thr Ser Pro Pro Ser Pro Arg Gly Ser Pro Val
85 90 95Ala Ser Pro Val Lys Glu Ser Ser Ala Thr Ala Ser Thr Lys Met Pro
100 105 110Pro Pro Thr Pro Val Ser Thr Ala Met Thr Thr Ala Lys Trp Leu Arg
115 120 125Thr Val Ile Ala Pro Leu Pro Pro Lys Pro Ser Ser Glu Leu Gly His
130 135 140Phe Leu Ser Ser Cys Asp Arg Asp Ile Thr Ala Asp Val Ser His Arg145 150 155 160Ala Arg Ile Val Leu Glu Ala Ile Phe Leu Val Val Thr Trp Gly Lys
165 170 175Met Cys Cys Gly Ala Gln
180
<210>81
<211>142
<212>PRT
<213>辐射松(Pinus radiata)
<400>81Thr Asp Gly Phe Ser Tyr Phe Glu Gly Leu Met Glu Glu Thr Ser Leu1 5 10 15Asn Ser Ser Ile Arg Ile Leu Glu Ser Asn Tyr Met Asn Ala Ile His
20 25 30Asp Arg Gly Glu Leu Asp Glu Arg Met Phe Val Asn Asp Glu Asp Ser
35 40 45Leu Phe Gly Ser Val His Ala Ser Val Asp Ser Val Asn Ile Leu Gly
50 55 60Ala Lys Arg Lys Tyr Glu Ala Ile Ser Ser Pro Met Lys Arg Ile Thr65 70 75 80Ser Pro Leu Ser Ser Pro Val Ser Pro Ser Ala Ser Gly Thr Ile Tyr
85 90 95Ser Asn Val Lys Met Leu Pro Pro Thr Pro Val Ser Thr Thr Met Thr
100 105 110Thr Ala Lys Trp Leu Arg Thr Val Ile Ala Pro Leu Pro Ala Glu Pro
115 120 125Cys Lys Glu Leu Asn Ser Phe Leu Leu Leu Val Thr Glu Met
130 135 140
<210>82
<211>61
<212>PRT
<213>辐射松(Pinus radiata)
<400>82Thr Gly Gly Asn Gly Arg Lys Leu Ile Trp His Gly Val Pro Arg Ser1 5 10 15Ile Arg Asp Cys His Arg Lys Val His Asp Ser Ser Asp Gly Leu Ile
20 25 30Ile Gln Arg Asp Val Ala Leu Phe Phe Ser Gly Gly Asp Ile Asn Glu
35 40 45Leu Asn Leu Arg Leu Thr Gly His Ile Leu Lys Glu Gln
50 55 60
<210>83
<211>325
<212>PRT
<213>辐射松(Pinus radiata)
<400>83Met Gly Trp Phe His Arg Cys Phe Gly Phe Ile Arg Lys Lys Lys Lys1 5 10 15Gln Lys Ser Pro Lys Ser Glu Pro Pro Ser Arg Glu His Leu Leu Lys
20 25 30Ser Thr Gln Glu Glu Phe Glu Asn Thr Lys Gly Ala Gln Tyr Lys Tyr
35 40 45His Arg Arg Phe Pro Ala Val Arg Asp Lys Thr Glu Gln Val Ala Thr
50 55 60Arg Ser Phe Gly Asp Leu Asp Gly Ala Ser Leu Ile Glu Thr Pro Gly65 70 75 80Arg Glu Thr Leu Gln Ile Val Ile Thr Glu Cys Pro Asn Thr Arg Thr
85 90 95Val Cys Ser Gly Cys Lys Ser Arg Leu Ser Asn Trp Cys Pro Ser Cys
100 105 110Arg Cys Asn Leu Gly Asn Phe Arg Cys Leu Ala Pro Glu Thr Glu Thr
115 120 125Ser Ser Gln Glu Leu Thr Cys Met Tyr Gln Ser Tyr Gly Cys Glu Asp
130 135 140Met Tyr Pro Tyr Tyr Ser Glu Leu Arg His Glu Ala Gln Cys Asn Phe145 150 155 160Arg Pro Tyr Asn Cys Pro Tyr Ala Gly Ser Glu Cys Lys Leu Val Gly
165 170 175Asp Ile Pro Phe Leu Val Ala His Leu Arg Asp Asp His Lys Val Tyr
180 185 190Met His Asn Ser Cys Thr Phe Asp His Arg Tyr Val Lys Ser Asn Pro
195 200 205Leu Glu Val Glu Asn Ala Ile Trp Met Pro Thr Val Ile Asn Cys Phe
210 215 220Gly Gln Phe Phe Cys Leu His Phe Glu Ala Phe Leu Leu Asp Met Ala225 230 235 240Pro Val Tyr Ile Ala Phe Leu Ile Phe Met Gly Asp Asp Asn Glu Ala
245 250 255Lys Asn Phe Ser Tyr Cys Leu Glu Thr Gly Gly Asn Gly Arg Lys Leu
260 265 270Ile Trp His Gly Val Pro Arg Ser Ile Arg Asp Cys His Arg Lys Val
275 280 285His Asp Ser Ser Asp Gly Leu Ile Ile Gln Arg Asp Val Ala Leu Phe
290 295 300Phe Ser Gly Gly Asp Ile Asn Glu Leu Asn Leu Arg Leu Thr Gly His305 310 315 320Ile Leu Lys Glu Gln
325
<210>84
<211>120
<212>PRT
<213>辐射松(Pinus radiata)
<400>84Phe Lys Gly Arg Asn Ser Asp Gln Val Ser Val Asp Leu Gln Val Phe1 5 10 15Arg Cys Leu Gly Gln Tyr Phe Cys Leu His Phe Glu Ala Phe Gln Leu
20 25 30Gly Met Ala Pro Val Tyr Ile Ala Phe Leu Arg Phe Met Gly Asp Asp
35 40 45Asn Glu Ala Lys Asn Tyr Ser Tyr Ser Leu Glu Val Gly Gly Asn Gly
50 55 60Arg Lys Met Ile Trp Gln Gly Val Pro Arg Ser Ile Arg Asp Ser His65 70 75 80Arg Lys Val Arg Asp Ser Phe Asp Gly Leu Ile Ile Gln Arg Asn Met
85 90 95Ala Leu Phe Phe Ser Gly Gly Asp Arg Lys Glu Leu Lys Leu Arg Val
100 105 110Thr Gly Arg Ile Trp Lys Glu Gln
115 120
<210>85
<211>197
<212>PRT
<213>辐射松(Pinus radiata)
<400>85Met Ala Asp Gln Ala Leu Glu Gly Ser Gln Pro Val Asp Leu Ser Lys1 5 10 15His Pro Ser Gly Ile Val Pro Thr Leu Gln Asn Ile Val Ser Thr Val
20 25 30Asn Leu Asp Cys Lys Leu Asp Leu Lys Ala Ile Ala Leu Gln Ala Arg
35 40 45Asn Ala Glu Tyr Asn Pro Lys Arg Phe Ala Ala Val Ile Met Arg Ile
50 55 60Arg Glu Pro Lys Thr Thr Ala Leu Ile Phe Ala Ser Gly Lys Met Val65 70 75 80Cys Thr Gly Ala Lys Ser Glu Gln Gln Ser Lys Leu Ala Ala Arg Lys
85 90 95Tyr Ala Arg Ile Ile Gln Lys Leu Gly Phe Pro Ala Lys Phe Lys Asp
100 105 110Phe Lys Ile Gln Asn Ile Val Gly Ser Cys Asp Val Lys Phe Pro Ile
115 120 125Arg Leu Glu Gly Leu Ala Tyr Ser His Gly Ala Phe Ser Ser Tyr Glu
130 135 140Pro Glu Leu Phe Pro Gly Leu Ile Tyr Arg Met Lys Gln Pro Lys Ile145 150 155 160Val Leu Leu Ile Phe Val Ser Gly Lys Ile Val Leu Thr Gly Ala Lys
165 170 175Val Arg Asp Glu Thr Tyr Thr Ala Phe Glu Asn Ile Tyr Pro Val Leu
180 185 190Thr Glu Phe Arg Lys
195
<210>86
<211>158
<212>PRT
<213>辐射松(Pinus radiata)
<400>86Met Ala Glu Gln Val Leu Glu Gly Ser Gln Pro Val Asp Leu Glu Lys1 5 10 15His Pro Ser Gly Ile Val Pro Thr Leu Gln Asn Ile Val Ser Thr Val
20 25 30Asn Leu Asp Cys Lys Leu Asp Leu Lys Ala Ile Ala Leu Gln Ala Arg
35 40 45Asn Ala Glu Tyr Asn Pro Lys Arg Phe Ala Ala Val Ile Met Arg Ile
50 55 60Arg Glu Pro Lys Thr Thr Ala Leu Ile Phe Ala Ser Gly Lys Met Val65 70 75 80Cys Thr Gly Ala Lys Ser Glu Gln Gln Ser Lys Leu Ala Ala Arg Lys
85 90 95Tyr Ala Arg Ile Ile Gln Lys Leu Gly Phe Pro Ala His Phe Lys Asp
100 105 110Phe Lys Ile Gln Asn Ile Val Gly Ser Cys Asp Val Lys Phe Pro Ile
115 120 125Arg Leu Glu Gly Leu Ala Tyr Ser His Gly Ala Phe Ser Ser Tyr Glu
130 135 140Pro Glu Leu Phe Pro Gly Leu Ile Tyr Arg Met Lys Gln Pro145 150 155
<210>87
<211>213
<212>PRT
<213>辐射松(Pinus radiata)
<400>87Arg Val Tyr Leu Ser Leu Ser Cys Ala Leu Val Thr Ala Ala Ile Gly1 5 10 15Val Tyr Leu His Leu Leu Leu Asn Ile Gly Gly Leu Leu Thr Gly Leu
20 25 30Ala Cys Ile Gly Ser Val Ile Gly Leu Leu Ser Val Pro Thr Ser Ser
35 40 45Asn Asn Glu Gly Lys Arg Ala Ala Leu Leu Leu Ala Ala Ala Ala Phe
50 55 60Lys Gly Ala Thr Leu Gly Pro Leu Ile Asp Ala Val Ile Asn Ile Asp65 70 75 80Ser Ser Ile Leu Val Ser Ala Phe Val Gly Thr Ser Leu Ala Phe Ala
85 90 95Cys Phe Ser Ala Ala Ala Ile Thr Ala Arg Arg Arg Glu Tyr Leu Phe
100 105 110Leu Gly Gly Leu Leu Gly Ser Gly Ile Ser Ile Leu Met Trp Leu Gln
115 120 125Leu Ala Ser Ser Ile Phe Gly Gly Ser Ser Ala Ile Tyr Thr Phe Glu
130 135 140Ile Tyr Phe Gly Leu Leu Val Phe Leu Gly Tyr Ile Ile Phe Asp Thr145 150 155 160Gln Met Ile Ile Glu Lys Ala Asp His Gly Asp Tyr Asp Tyr Leu Lys
165 170 175His Ser Leu Asp Leu Phe Ile Asp Phe Val Ala Val Phe Val Arg Leu
180 185 190Met Val Ile Met Ala Lys Asn Ala Asp Ser Lys Ser Arg Glu Gly Lys
195 200 205Lys Lys Arg Arg Ala
210
<210>88
<211>140
<212>PRT
<213>辐射松(Pinus radiata)
<400>88Met Asp Ala Phe Ala Ser Leu Phe Gln Ser Ser Gly Lys Gly Trp Ser1 5 10 15His Asp Ser Leu Lys Asn Phe Arg Gln Ile Ser Pro Ala Val Gln Ser
20 25 30His Leu Lys Asn Val Tyr Leu Ser Leu Cys Cys Ala Leu Met Ala Ser
35 40 45Ala Gly Gly Ala Tyr Leu His Leu Met Leu Asn Ile Gly Gly Leu Leu
50 55 60Thr Thr Ile Ala Cys Ile Gly Ser Ile Val Trp Leu Leu Ser Ile Pro65 70 75 80Pro His Glu Glu Gln Lys Arg Phe Gly Leu Leu Met Ala Ala Ala Leu
85 90 95Phe Glu Gly Ala Cys Ile Gly Pro Leu Ile Glu Ala Ala Ile Lys Val
100 105 110Asp Pro Ser Ile Val Ile Ser Ala Phe Val Gly Ser Ala Leu Ala Phe
115 120 125Ala Cys Phe Ser Gly Ala Ala Cys Trp Leu Gly Gly
130 135 140
<210>89
<211>245
<212>PRT
<213>辐射松(Pinus radiata)
<400>89Met Lys Thr Thr Asn Ala Gly Ser Ile Met Leu Val Leu Ile Ile Phe1 5 10 15Gly Cys Cys Phe Ile Gly Val Ile Ala Thr Ser Phe Asp Phe Tyr Tyr
20 25 30Phe Val Gln Gln Trp Pro Gly Ser Tyr Cys Asp Thr Arg Arg Gly Cys
35 40 45Cys Tyr Pro Arg Thr Gly Arg Pro Ala Ser Glu Phe Ser Ile His Gly
50 55 60Leu Trp Pro Asn Tyr Lys Thr Gly Lys Trp Pro Gln Phe Cys Gly Ser65 70 75 80Ser Glu Glu Phe Asp Tyr Ser Lys Ile Ser Asp Leu Glu Glu Glu Leu
85 90 95Asn Arg Tyr Trp Gly Ser Leu Ser Cys Pro Ser Ser Asp Gly Gln Glu
100 105 110Phe Trp Gly His Glu Trp Glu Lys His Gly Thr Cys Ser Leu Asn Leu
115 120 125Asp Glu His Ser Tyr Phe Glu Lys Ala Leu Ser Leu Arg Gln Asn Ile
130 135 140Asp Ile Leu Gly Ala Leu Lys Thr Ala Gly Ile Lys Pro Asp Gly Ser145 150 155 160Gin Tyr Ser Leu Ser Asp Ile Lys Glu Ala Ile Lys Gln Asn Thr Gly
165 170 175Gln Leu Pro Gly Ile Asp Cys Asn Thr Ser Ala Glu Gly Glu His Gln
180 185 190Leu Tyr Gln Val Tyr Val Cys Val Asp Lys Ser Asp Ala Ser Thr Val
195 200 205Ile Glu Cys Pro Ile Tyr Pro His Ser Asn Cys Pro Ser Met Val Val
210 215 220Phe Pro Pro Phe Gly Glu Asp Gln Glu Asp Arg Asp Gly Tyr Thr Glu225 230 235 240Gly Met Tyr Glu Leu
245
<210>90
<211>1323
<212>DNA
<213>辐射松(Pinus radiata)
<400>90actttttctc ttaatttgtg aatccattac aatgatggta tgcaggtgaa aggaatagga 60gtgggattct tattaagcaa tggaaggtta cgctgcgaat aacgatgcag aacttctgag 120caaaaccctt caagtggaac agaagttgtt ctatttcgat ctcaaggaaa acccccgagg 180tcaatacctt aaaatctctg agaagacctc cggctcacgg tctacaataa ttgtgcccat 240tggtggagtt gcatggttcc tcgatctctt taattattat gtcgacggag atgacgagga 300agttttgagc aaggaattgc agctggatgc caaggtattt tatttcgatg ttggggtgaa 360taaaaggggt cggttcttga agatttctga agcatctaca tcctacagtc gcagcacaat 420cattgtacct gtaggaaaca caagaaaaga tggttgggca gcatttagaa atattttagg 480agagataaat gaagcttccc aacaagcttc tggcccatcc gaacatttgg gagggctttc 540cgatgaagtt ggtgctggtt tcctatcagg tggaagtgga gaagcagcat ttgaggcaga 600cacaactggt gacagagcca tgggtttaac tccagcagaa gatacgggtg aagcagttgt 660ttcaaaagtg attcgagctg atcagaaacg tttctttttt gatcttgggt gtaacaaccg 720gggccagttt cttagaattt ctgaggtgat aggtcctgac cgttcagcta ttattgttcc 780tgtatctgct ttggagcagt ttcatgatgt gctaggccat tttgttgaca tcaccagaac 840tcagggtctt gcagctgcaa gtggtgcaaa tgtgcgcaca gtagcagctg cacacagacg 900aaatgaaaac tagattacct gcatataaac cgaaatgtga tttgattgga ctgatcatag 960ggagcattgg aaatggtcaa tgacacttca caagtttatg actgtcattt gcggccattt 1020catcatgcaa ataatcttct ttgttgccat cacattatgt gagtataacc tgtttgttga 1080agtgctactg aagcacatga ccattataac ctttgctatt ggtggacttt tgaaggaaag 1140gcaccaatga aacttcatgg gttatttgag gtttacatat atggttggcg gatctatatc 1200acttaaggag agtttttatg caacagcatt tttgtttcta tcagaaggct gctttaatcc 1260agaagccatt aaacaatttc aaaatgaaat aaatttgtct taaatttcaa aaaaaaaaaa 1320aaa 1323
<210>91
<211>344
<212>DNA
<213>辐射松(Pinus radiata)
<400>91aaattaatgg actcagcgag gtgaaaggaa taggagtggg attcttatta agcaatggaa 60ggttacgctg cgaataacga tgcagaactt ctgagcaaaa cccttcaagt ggaacagaag 120ttgttctatt tcgatctcaa ggaaaacccc cgtggtcaat accttaaaat ctctgagaag 180acctccggct cacggtctac aataattgtg cccattggtg gagttgcatg gttcctcgat 240ctctttaatt attatgtcga cggagatgac gaggaagttt tgagcaagga attgcagctg 300gatgccaagg tattttattt cgatgttggg gtgaataaaa gggg 344
<210>92
<211>1463
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>92cctcctcctc ctcctccttc ttccttcttc ttcctccaat cttcctcctt catagtcgtc 60gtccatccat ggcgatcccg gccaccgccg ccgcggccct cctcctcttc tgcgccgtcg 120ccgccgtcgc tgcggccgcg aggaccgacg aggaggtcat gggcatgtac gagctctggc 180tcgccaagca cggcaaggcc tacaacggcc tcggcgagcg ggagcggcgg ttcgagatct 240tccgcgacaa cctccggttc gtcgacgagc acaacgccct caaccggtcg tacaccctcg 300ggatgaaccg gttcgccgac ctcaccaacg aggagtaccg cgccgtctac ctcggcaccc 360ggagcgaccc catgcgccgg gtggcgaagg cggcgcgcgc gagcggccgc tacgcccccc 420gccccgacga catgctgccg gcagccgtcg actggaggac ccgtggcgcc gttaacaagg 480tcaaggacca gggagcttgc ggaagctgct gggccttctc taccatagct gctgtggaag 540gaatcaacca gatcgtcacc ggcgagttca tatccctctc cgagcaagag ctcgtggatt 600gtgaccgggc ctacgatgcc ggttgcaatg gtgggctcat ggactatgcc ttccagttca 660tcattgacaa tggtggcatt gacactgatg aggactactc ttacacggga gtcgatggaa 720cctgtgatgc gtctaaggtt aactcaaagg tggtgagcat tgatgggtac gaggatgtcc 780cggcctttga cgagagagcc ttgaagaagg ctgtggctca tcaacccgtg agtgttgcca 840ttgaagctgg aggcagagat ttccaacttt atgaatccgg cgtgttcact ggagaatgtg 900gaactgcact ggaccacggt gtgatcgcag ttggatacgg gaggcaacat ggtgctgact 960actggcttgt aaggaactcg tggggctcct tgtggggcga gagcggatac atcaagatgg 1020agaggaactt ggccaacaac tactttggca agtgcggtat cgcaatggag gcttcttacc 1080ctgtcaagac cactcagaac cctgctagta aatattcttc aatgggaagc agtggtggca 1140tcgagctcgt cagcagttct tgaagcccgg aatagaggaa aacttacaga aaggtgcaag 1200attgattgtc tttgagttcg gggatcactc tgggttcaca taattgtgtt atattccttc 1260ccgggggtcg gtttatggaa gggggaaagg aatatggatg cacatcgggc ggttttctta 1320tgattctact gtaaatatct ttcatttcgt caacatttga attccgaaga attgcaccat 1380aagaagcaat agttattgta actcgaactg cctagttcaa gtcttcaatt gaaactcctc 1440tctttcacaa aaaaaaaaaa aaa 1463
<210>93
<211>330
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>93ccttcctcca atcttcctcc ttcatcgtcc ttgtccatcc atggcgatcc cggccgccgc 60cgccgccgcc ctcctcctct tctccgccgt cgccgccgtc gccgccgccg ctgcgaggac 120tgacgaggag gtcatgggca tgtacgagct ttggctcgtc aagcacggca aggcctacaa 180cggcctcggc gagcgggagc ggcggttcga gatcttccgc gacaacctcc ggttcgtgga 240cgagcacacc ggcctcaacc ggtcgtacgc cctcgggatg aaccggttcg ccgacctcac 300caacgaggag taccgcgcca tctacctcgg 330
<210>94
<211>886
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>94cagatcgtca ccggtgagct catatccctc tctgagcaag agcttgtgga ttgtgaccgg 60tcctatgatg ccggttgcaa tggtgggctc atggactatg ccttccagtt catcattgac 120aatggtggca tcgacactga tgaggactac tcttacacgg gagtcgatgg aacctgtgat 180gcgtccaagg ttaactcaaa ggtggtgagc attgatgggt acgaggatgt cccggccttt 240gacgagagag ccttgaagaa ggctgtggct catcaacccg tgagtgttgc cattgaagct 300ggaggcagag atttccaact ttatgaatcc ggcgtgttca ctggcgaatg tggaactgca 360ctggaccacg gtgtgatcgc ggttggatac gggaggcaac atggtgctga ctactggctt 420gtaaggaact cgtggggctc cttgtggggt gagagcggat acatcaagat ggagaggaac 480ttggccaaca actactttgg caagtgcggc atcgcaatgg aggcttctta ccctgtcaag 540acctcccaga accctgctag taaatattct tcaatgggaa gcagcggtgg catcgagctt 600gtcagcagtt cttgaagccc ggaatagagg aaaacttaca gaaaggtgca agatcgattg 660tctttgagtt cggggatcac tctgggttca cataattgtg ttatattcct tccggggggt 720cggtttatgg aagggggaaa ggaatatgga tgcacatcgg gcggttttct tatgattcta 780ctgtaaatat ctttcatttc atcaacattt gaattccgaa gaattgcacc ataagaagta 840atagttattg taactcgaac tgcctagttc aagtcttcaa ttgaaa 886
<210>95
<211>948
<212>DNA
<213>辐射松(Pinus radiata)
<400>95acaaagtggt caggcccttt tgcttctctt ggtcaatgcc ctctctgcgt taacatttcg 60ttcgcagcaa gccaccggga aaggcctgat ccagccaaaa gaagtacgga tctcgaggtg 120gaagatctac tgtagatatg ggaattcttc tgttctttgc tctgctggca atgttagcca 180tggctggcaa tgcatcaaga gcggattttt ccatcatcag caacaaggat ctgagagaag 240atgacgcaat catggagctc tatgaactgt ggcttgcaga gcacaaaaaa gcctacaatg 300gtcttgacga gaagcagaag aggttcactg tattcaaaga caattttctg tatattcacg 360agcacaacca ggggaatcgg tcctacaaac tgggtctgaa ccagtttgca gatctgagcc 420acgaggaatt caaggccaca tatctgggtg ccaagctgga tactaagaaa cgcttgctga 480ggtctcccag ccctcgatac cagtattccg acggcgagga tttgccaaag tccattgact 540ggagagaaaa gggagccgtg gctcctgtga aggaccaggg tgcatgtgga agttgttggg 600cgttctcaac tgtggcggcc gttgaaggaa tcaatcaaat cgtgaccggc gatttgattt 660cgctgtccga gcaagaactg gtggactgtg atacttctta caaccaagga tgcaacggtg 720gcctcatgga ttacgctttc gagtttatca taaacaatgg tggacttgac agcgaggagg 780attaccccta cacggcctac gatggatcat gtgacgctta caggaaaaat gcccatgtgg 840tgacaatcga tgactacgaa gatgtgcctg aaaacgatga gaaatcgttg aagaaggctg 900cggctaatca gccaattagc gttgccatcg aagccagcgg gagggagt 948
<210>96
<211>883
<212>DNA
<213>辐射松(Pinus radiata)
<400>96gtggtcaggc ccttttgctt ctcttggtca atgccctctc tgcgttagca tttcgttcgc 60agcaagccac cgggaaaggc ctgatccagc caaaagaagt acggatctcg aggtggaaga 120tctactgtag atatgggaat tcttctgttc tttgctctgc tggcaatgtc agccatggct 180ggcagtgcat caagagcgga tttttccatc atcagcaaca aggatctgag agaagatgac 240gcaatcatgg agctctatga actgtggctt gcagagcaca aaaaagccta caatggtctt 300gacgagaagc agaagaggtt caccgtattc aaagacaatt ttctgtatat tcacgagcac 360aaccagggga atcggtccta caaactgggt ctgaaccagt ttgcagatct gagccacgag 420gaattcaagg ccacatatct gggtgccaag ctggatacta agaaacgctt gctgaggtct 480cccagccctc gataccagta ttccgacggc gaggatttgc caaagtccat tgactggaga 540gaaaagggag ccgtggctcc tgtgaaggac cagggtgcat gtggaagttg ttgggcgttc 600tcaactgtgg cggccgttga aggaatcaat caaatcgtga ccggcgattt gatttcgctg 660tccgagcaag aactggtgga ctgtgatact tcttacaacc aaggatgcaa cggtggcctc 720atggattacg ctttcgagtt tatcataaac aatggtggac ttgacagcga ggaggattac 780ccctacacgg cctacgatgg atcatgtgac gcttacagga aaaatgccca tgtggtgaca 840atcgatgact acgaagatgt gcctgaaaac gatgagaaat cgt 883
<210>97
<211>1066
<212>DNA
<213>辐射松(Pinus radiata)
<400>97caggaacgcc cacccttttc attttgaggg tgactgcttt gcttggtcca tccatgtggc 60tttgaatcct ttgagagact ggtttggttt tcagcgaggg ttacgcccag accggtggag 120agaaattccc tatttgtgcc caaatcagtc aaaaactaag attgtatcag tatgggaatc 180cttctgctgt ttgctctgct ggcactgttt gccatggcag gcagtgcttc cagagcagat 240ttttccatca tcgggtatga tagcaaagat ctgagggaag atgatgcgat catggagctt 300tatgaactgt ggctcgctca gcacaggaaa gcctataatg gccttgacga gaaacagaag 360aggttctctg tatttaaaga caattttctg tatattcatc agcacaacaa ccaagggaac 420ccatccttca aaatggggct gaaccagttt gcagacctaa gtcacgagga attcaaggcc 480acatatttgg ggtgcgaact ggataccaag aaacgcttgt ccaagtctcc cagccctcga 540taccagtatt cggagggcga gaatttgcca gagtccgttg actggagaga aaagggagcc 600gtggctgctg ttaaggacca gggctcctgc ggaagttgtt gggcgttctc gacggtggct 660gccgttgaag gcatcaatca aattgtgact ggcaatttga cttccctgtc cgagcaggaa 720ctggtggatt gcgatacttc ttacaaccaa ggatgcaatg gcggtctcat ggattatgct 780ttccagttta tcatagacaa cggtgggctt gacagtgagg atgattaccc ttacatggcc 840aacgatggca gctgtgacgc ttaccgaaaa aatgcccatg tggtgacaat tgatagctac 900gaagatgtgc ctgagaacga tgagaagtcg ctgaagaagg ccgcggcgca tcagccgatt 960agcgttgcca tcgaagccag cggaagggcg ttccagtttt acgaatctgg cgtgttcaca 1020agcacctgcg gaactcagct ggaccacggt gtcactctgg tagggg 1066
<210>98
<211>1600
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>98attttctctc tctatacttc tctctctctc ctctgatgct ccgatcggat acgccgaagc 60caagaagatg gatctcaaat cgccgccggc ggcggccgcc gtggccgtcc tcgccctcgc 120gctggccctg acgacgatcg cctccgccct cgacatgtcc atcgtcagct acgatcgggc 180ccacggcgac cggtcctcct cctcctcctc ctcctggagg tccgacgacg aggtgatggc 240cgtctacgag agctggctcg ccaagcacgg caaggcctac aacgccctgg gcgagaagga 300gaagcgcttc caggtcttca aggacaacct ccggttcatc gacgaccaca acgccggcgg 360ggaccggacc tacacggtcg gcctcaacca gttcgccgac ctcactaacg aggagtaccg 420gtccatgtac ctgggcgcca ggatggatcg gtcggggcgg cggctcggga gggcccgcag 480cgatcggtac gccgtggccg ccggggagga gctgccggcg tccgtcgatt ggaggaagga 540aggcgccgtt gttgacgtca aggaccaggg aagctgcggg agttgctggg cgttctctac 600aattgctgct gtggagggga taaacaagct tgtgactggt gatttgatct ctctgtccga 660gcaggaactt gtggactgcg atacatccta caatgaagga tgtaatggcg ggctcatgga 720ttatgccttt gaattcatta tcaacaacgg aggcattgat accgaggaag attatcccta 780tagagctgta gatagcactt gtgaccaata caggaagaac gcaaaggttg tgacgattga 840cgattatgaa gatgttccag aaaatgatga gaaagcattg caaaaggcag ttgctaatca 900accagtcagt gtggccattg aagcaggagg ccgggaattc cagttttatg attcgggtat 960atttactggc aaatgtggga cagctctgga tcatggggtt actgcagtcg gatatggcac 1020agaaaacgga gttgattact ggatagtgaa gaactcatgg ggcggtagct ggggagagca 1080agggtacatc aaaatggcac gaaatgtggc caatagcccc actggcaaat gtggtatagc 1140aatggaggcc tcctacccca tcaagaaagg ccaaaatcct cctaatcccg gcccatctcc 1200tccatctcca gtgaagcccc caactgtctg cgacaattac tactcttgtc ccgagagcaa 1260cacctgctgc tgtgtctatg agtatgcaaa ctactgcttt gcctggggat gctgccctct 1320cgaggcagcc acctgttgtg aagaccacta tagttgctgc cctcaagact tcccggtctg 1380caatgtaaac gctgggacct gccagatgag caaagacaat ccactaggag tcaaggcatt 1440gaagcgcact cctgctaaat ttcactgggc ttttggaagt gatggacaga agagcagtgc 1500ataaaaaaaa aacttgggat tgtatgctgt agatggagat tcttaaggag agtcaagaaa 1560atcacagcga ctcatcttct cctcattctc attaaactgc 1600
<210>99
<211>700
<212>DNA
<213>辐射松(Pinus radiata)
<400>99ggatcaaggc gactgtggaa gttgttgggc attctcggca gcggcagcta tggaagggat 60taccatgatc aagaagggga agttggtgcc actctcagta caagaactcg tggattgtga 120cgttgacgat aatggctgcc atggcgggct catggacagg gccttcaagt ttatcaagag 180caagggcgga ctctcgactg aggcgaacta cccctaccag gcaaataacg gaacctgcaa 240tacggccaag atggcaaacc ccgtggcctc aataactgga taccaggatg tgccggccaa 300taacgaaaag gccctcttgc aggccgtggc aaaccagcca gtctcggtgg caattgaggg 360gagcgggttc aatttccaat tctactcaag cggtgtgttc tctggctcgt gtggaaccag 420tatcgaccat gccgtcacgg cggtcgggta tgggaagacc tccaggggaa ccaagtattg 480gctgctgaag aattcatggg gcactggatg gggtgagagc gggtacatga ggatccagag 540ggacgtgagt tctaacgctg gtctctgtgg ccttgccatg gaagcttctt atccaaccgc 600atgaagaaag aagaagaaga aaaaatacag tatgagctta tataagtgat actggttctt 660aggctctcta tacgtatgaa ataatgtcta gtgtctctgt 700
<210>100
<211>739
<212>DNA
<213>巨杉(Eucalyptua grandis)
<400>100caattacttg aggacgaaca agctcctctc tctttccgag caagagctgg tggactgcga 60caacacacaa aaccacgggt gcaacggcgg gctcatggat attgcattcg aattcatcaa 120gcagaaggga ggcatcacgt ccgagtcgaa ttacccttac caagcaagca atgggacctg 180tgacgctgcc aaggagaatt ccccggtggt gtcgatcgat ggacatgaaa atgttcccgc 240caacgacgag gatgcgttgc agaaggcggt cgctaatcag cctgtgtctg tggccataga 300agcaagcggt gcagattttc aattctactc cgagggtgta ttcactggaa gctgtggcac 360acacctagat cacggagtcg cgattgtcgg ctacgggagc actctccagg ggaccaagta 420ctggattgtg aggaactcct ggggtccaga atggggcgag aagggctacc taaggatgga 480gcgtgggatc gaagctaagg aagggctgtg cggcatagcc atggaggcct cataccccat 540caagaactcc tcagataatc ccgccggagt ttcatctcct gtcaaggatg aactctaggc 600aatgactcaa gattgactag tgatcacagt ttcttaggct ttcgtttgtg tcctctgctt 660gatagtgtgc atgtcctgtg tccacataga cacaaataat gtctgctccc tatgattaca 720cagtgaatgt ttatagggc 739
<210>101
<211>1474
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>101aagacacagg gaagaagaaa agaatccgtg caaaatcatc tgctaaaaga aagttacaac 60tcaccaagtc actccaattg tgtcctagac agtacccaga gcacgactat ggccaaacaa 120aaccaattct cattcctcac atcagctgct cttttg0tca ttata`tttc tgtttcggaa 180acgctttgtc gccctcttga agaggaacag ttgttaaagc aacatgagga gtggatggca 240atccacgggc gtgtctacaa ggacgcggtc gaaaaggcaa aacggtatga gatatttaaa 300gagaacgtta agcgcatcaa cgcctttaat aatggtaagg acgtggggta taaaatggct 360gtgaataagt tcgcagacct aaccaatgag gagttccgcg cttcctacac cggctacaag 420aggaggccca caagggtcct gtcctctggc gaaaaaaaac cgttcaagta tgccaacttc 480accgccattc cagctgcctt agactggcga accaagaagg ctgtgacacc tgttaaggat 540caaggcggct gtggaagttg ttgggcattc tcggctgtgg cagctatgga agggattacc 600atgatcaaga aggggaagtt ggtgccactc tcggtgcaag aactcgtgga ttgtgatgat 660aacgatgaag gttgcagggg cgggctcatg gacagtgcct tcaagttcat tgtaagcaat 720ggtggcctca cgactgaggc gaactatcct taccagggaa atgacgggac ctgcaatacg 780gccaagacgg caaaccccgc agcctcaata actgggtatc aggatgtgcc ggccaacaac 840gagaaggccc tcttgcaggc cgtggcaaac cagccagtct cggtggcaat tgaggggggc 900gggtataatt tccaattcta ctcaagcggc gtgttcacag gctcatgtgg gaccgacatc 960gaccacgcgg tcacggcagt cgggtatggg aagacctctg gctctggcgg gaccaagtat 1020tggctgatga agaattcgtg gggcaccggg tggggagaga aagggtacat gaggatccag 1080aaggatgtga gctctaaggc tggtctctgt ggtcttgcca cggaagcttc ttatccagct 1140gcatgaagaa ataagaagat gaaaaaataa attatgagtc catatatgtg atagtggttc 1200ttgggctctc tatatgtatg aaatgtagtc tagcgtctct gtaaaagaag caagttttaa 1260gtgacagtac gtcactaggg ttggtgtgtc ctacaacaga aactaaatgt tgtaagcaaa 1320attaggagcc tactaactaa acttggttct aagtgttcaa ataaatgtgt gggacaagat 1380aaaagaacag tcccgtgaat gaatggaaca gtttcgtcag cgtaatttta tagtaaaaaa 1440aaaaaaaaaa aaaactcgag actaggtctc tctc 1474
<210>102
<211>2167
<212>DNA
<213>辐射松(Pinus radiata)
<400>102aagaaaatcg tcttcccctt tctccacaca aattctcatt atctctctga attttgttgt 60tatttcgtga tggcgagtgt ttcaaaggca accctgttac tcttccttgc gaccaccttg 120tggactttat cagccaatgc ttcggattct tcccccggat ttacagatga agacctcaag 180tctgaggaaa gtctgcgact cctttatgac aaatgggcac ttcggcatcg caccaccaga 240agtttggatt cggatgagca tgccaaacga ttcgagatat tcaaagacaa tgtgaaatat 300atcgactccg tgaatcagaa ggatggtcca tacaaacttg gattgaataa gtttacagat 360ctgagtaacg aagaattcaa ggccatgcac atgacaacta gaatggagaa gcacaagagt 420ctgcgccgag acagaggaac acacagtgga tcattcatgt accaaaactc cgacaacctg 480ccagagtcta ttgattggag agaaaaggga gccgttaatc ctgtgaagaa ccaaggccaa 540tgtggaagtt gttgggcatt ttcaactata gcttctgtag agggcattag ctatgtcaaa 600acagggaagc tggtttcttt atccgagcaa cagttggtag attgcagtaa agagaacgca 660ggctgcaacg gagggcttat ggacagcgcc ttccaatata tcatagataa tggtgggatc 720gttgctgaag atgagtatcc atatactgct gaagccagcg aatgcagtcc ctccaaggtt 780aaaccgaatg caatagcagc aactattgat ggttttgaag atgttcctgc taacaatgaa 840aaagctctta aagaagcagt gggccaccaa cctgtgtctg tcgccattga agcaagtggt 900aaagattttc aattttattc aaaaggagta ttcactggtg aatgtggcac tgaacttgac 960catggagttg tagccgttgg ttatggcaag tctcctgagg gaattaatta ttggatagtt 1020aggaactctt ggggacctga atggggagaa gaagggtata taaaaatgca acgagatatt 1080gaagcagtag aagggaagtg tggtattgcc atgcaagctt catatccgac gaagaaaaca 1140caaggcattg atattgaact ggatgtcgct catgttagtg acgaactgtg aaaattgtct 1200caacaaaaaa ttggtattgt gaattcaata atatgaagat tcgttcttca tttattagtt 1260aaatttagtc attatactac attattgttc ataggtacgc gttccacgag cgccacgaga 1320agaaacaaga cgaggaaacc gaggaagccg aaggtggccg caagcctcgc cacaatctct 1380tctgaatttg gccatgcctc tctaatggtg gacttttgga gcaaaagcca attatagcag 1440ttggtgatta ctatgttcag tgttaagtag taataaagtt atgtaatttt ttttcagtgg 1500acttgttaca gtaaggtagt taggtgctgt cgttacttga gttacagttc agttgaattt 1560gtgatatgta tctctgtata tgagtgaata taaagtacct cagatcactg tttgctttga 1620cattgatgcc aatggtatcc tgaacgtctc tgctgaggat aaaacgacgg ggcagaaaaa 1680caagatcacc attaccaatg acaagggcag gctaagcaag gatgagatag aaaagatggt 1740tcaggatgca gagaaataca aggctgagga tgaagagctc aaattgaagg tagaagctaa 1800aaattctcta gagaactatg catacaacat gaggaatacc atcaaagatg ataagattgc 1860aggaaagttg gaccctgctg acaagaagaa aatcgaggat gcagttgatg gaattatcag 1920ctggttggat ggaaaccagt tggccgagaa ggaagagttt gaggataagt tgaaagagct 1980ggagtctact tgcaatccta tcattgcaaa aatgtaccag ggtgaagggg gtgcgggatt 2040tccaggtgct gatgcttttg gtggagcttc tggagctggt gacgagggtg caagtggccc 2100tggtcccaag atcgaggaag tcgactagat atatttcttc atcacctcct acgtgttttt 2160tttgtta 2167
<210>103
<211>557
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>103gaagattatc cgtataaagc tgtcgatggc aaatgtgacc aatatcgaaa gaatgccaaa 60gttgttacga tagatgatta tgaagatgtt ccagcgaacg atgagaaagc attgcaaaag 120gcagttgcta atcaaccagt gagtgtggcc attgaagccg gtggtcgggc atttcagttg 180taccagtcgg gtgttttcag tggacgatgt ggtactgcac tggaccacgg agtgactgcc 240gtaggatatg gcacagaaaa aggtatgaat tactggatcg taaagaactc ttggggcaaa 300agctggggag agcagggtta catcagaatg gagcgtagct tgaccaatac tataactggc 360aagtgtggga tcgcaatgga agcatcttac cccatcaaaa atggcccgaa tcccccgaac 420ccagggccat ctcccccgtc tccaataaaa ccaccgacca cttgtgatcg ttattactct 480tgtgctgaga gcacgacttg ctgctgcgtc tatcagtatg ccaactattg cttcgcctgg 540ggatgctgcc cgcttga 557
<210>104
<211>482
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>104catcaacacc caggaactcc tcccaagtca attctttcaa gtaccaaggt tcgaatagca 60tcccagagag catcgattgg gtccaaaaag gtgctgtcaa ccctatcaaa taccaacgtc 120aatgtggatc ttgctggtcg ttctccgtgg tggcggcagt agaagcaatc acacagatca 180ccactggcgt gctaccgagc ctgtccgagc agcaactcat agactgcacc actgatggga 240accacggctg tgaaggcggc tcaatggaca atggcttcga gtacatcatc aacaataacg 300gcatcagctc cgagacgaac tacccatacg ttggggtcga cggcacctgc aatgtgcagg 360cctcctctgt cgccgaggcc aaaatatcag accacaagga cgtcccctca aacgaggatg 420acatgctgaa ggccgtggcg atgcagccgg tgtcggcggc aatcgatgca aacggagacg 480tg 482
<210>105
<211>817
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>105agtcactcca attgtgtcct agatagtacc cacaccagta ctatggccaa acaaaaccaa 60ctcccattcc tcacattagc cactcttttg gtcatcatag tttttgtttc ggaaacgctt 120tgccgccccc ttggagagga acacctgtta aagcaacatg agcagtggat ggcggttcac 180gggcgcgtgt acaaggatgc ggatgaaaaa gcgaaacggt atgagatatt caaacagaac 240gttaaccgca tcaacgcctt taataatgat aaggacgcgg cgtataaact ggctgtgaac 300aagttcgcgg acctaaccaa cgaggagttc cgcgcttcct tcaccggcta caagaggagg 360tccacccgtg tcctgacctc tgtggacgag aaaccgttca agtacgcgaa cttcaccgct 420gctccacccg tcttggactg gcgaaccaag aaagctgtga catctgtcaa ggatcaaagc 480agctgtggag cttgttgggc gttctcggct gtggcggcta tggaagggat taccatgctc 540aagaagggga agttggtgtc actctcggag caagaactcg tggattgcga tgttaacggt 600gtcaaccaag gctgcgaggg cgggctcatg gacagtgcct tccagttcat caaaagcaag 660ggtggcctca cctccgaggc aaactaccct ttccagggga atgacgggac ctgcagaacg 720gccaaggcgg cgaatatcgt ggcctcgata gcaggctacc aggatgtgcc ggccaacaat 780gagaaggccc ttctgcaggc ccgtggcgaa ccagcca 817
<210>106
<211>368
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>106agtcactcca attgtgtcct agatagtacc cacaccagta ctatggccaa acaaaaccaa 60ctcccattcc tcacattagc cactcttttg gtcatcatag tttctgtttc ggaaacgctt 120tgccgccctc tcggagagga acacctgtta aagcaacatg agcagtggat ggcggttcac 180gggcgtgtgt acaaggacgc ggatgaaaaa gcgaaacggt atgagatatt caaacagaac 240gttaaccgca tcaacgcctt taataatgat aaggacgcgg ggtataaact ggctgtgaac 300aagttcgcgg acctaaccaa cgaggagttc cgcgcttcct tcaccggcta caagaggagg 360tccacccg 368
<210>107
<211>1407
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>107ccgacccaca tttcttggaa tccacagaga gagagagaga gagagagaga gagagaaatg 60gctcgcgcga ggctcctgtg ctccgccgtc ctcctcctcg tcgccgtcgt cgtctccgcc 120gcggcgtcga gcttcgagga gtccaacccc atccggctct tccccgacgg cggcctccgc 180gacctcgagt cctccatcgt ccagatcgtc ggccgcaccc gccacgcctt ctccttcgcc 240cgcttcgcca acaggtatgg gaagaggtac gagaccgcgg aggagatcaa gctgcggttc 300gagatcttca gggagaatct caagttgatc cgatccacca acaagaaggg cttgccctac 360accctcggtg tcaataagtt tgctgattgg agctgggagg agttcaggag gcacagactg 420ggagctgctc aaaactgctc tgccaccacc aagggcaacc acaagctcac cgacgaagct 480cttcccgaga tgaaagactg gagagaaaag ggcattgtaa gcccaattaa agatcagggg 540cactgtggat cttgctggac tttcagtacc actggagctc ttgaggctgc ttatcaccaa 600gcattcggga aacaaatctc tctgtctgag cagcagctcg tggactgtgc tggggctttc 660aacaactttg gatgtagtgg tggactgcca tcccaagcct ttgagtacgt caagtacaac 720ggtggccttg ataccgagga agcatatcct tataccgcag tggatggtag ctgcaaattc 780tcggctgata atgttggtgt ccaagtgctc gactctgtta acatcacctt gggtgctgag 840gatgaactaa agcatgcagt tgccttcgtc cggccagtga gtgtggcatt ccaggtcgtg 900aaagacttca gattgtacaa gtcgggtgtc tacacgagcg atacatgcgg tagcacttcc 960atggatgtga accatgctgt tctcgctgtt ggttatggag ttgaagatgg tgttccgttc 1020tggctcatca agaattcctg gggagcagac tggggtgacc acggatactt caagatggag 1080atgggaaaga acatgtgtgg agtcgctact tgtgcatcat accctgttgt ggcctagatt 1140gtttccagag gatcaatggg ttcgtgtgcc aggtaattcg agatatatat atatttcgca 1200tgaagaattg ctccgccctg agttggaatg ctatagtgtt agcacaaaag ggtttagctt 1260agtgcaaaaa ataatcatca ggacagctgc aactccatat ggttattgtt atatgaacaa 1320agaccttgta aatacctttc tatgtttgtg tggcatggaa cgctatcttt gcaagaataa 1380cgcatcaaat tcccaccaaa aaaaaaa 1407
<210>108
<211>388
<212>DNA
<213>辐射松(Pinus radiata)
<400>108agccatgcat atgaaaacca ggatggagaa gcacaggagc ctgcgcggag acagaggagt 60gcagggtgga tcattcatgt atcaaaactc taagcacctg ccggcgtcta tcgattggag 120aaaaaaggga gcggttactc cagtgaagaa tcaaggccaa tgcggaagtt gttgggcatt 180ttcaactgta gcttcggtag agggcattaa ctatatcaaa acagggaagc tggtttcttt 240atcagagcaa cagttggtag attgcagtaa agagaacgca ggttgcaacg gagggcttat 300ggacaacgcc tttcaatata tcatagataa tggcgggatc gttagtgaag cagagtatcc 360ttacactgcc gaagctaggg agtgcagt 388
<210>109
<211>1062
<212>DNA
<213>巨杉(Eucalyptus grandia)
<400>109gctcaaccca agttgagaga acatcatcat gggttcttct ccaagaactc atcatcaact 60aggtcttgcc atcctcttct ttgcttccct cgcgcgcctg tctctgtcac acgcctctct 120ccccagcgag tactcgatca tcggccatgg acaggacccg aatggcgccg tgtcggacga 180acgtgccgtc gagctcttcc ggagatggca ggcgcagcac aagaaggtgt acaagcacgc 240cggggaggcc gagaggcggc tcgagaactt caaaaggaac ctgaggtacg tgatggagag 300aagccggagg gatggcggca agcagcatgg cgtggggctg aacaagttcg ccgacctgag 360caacgaggag ttcaggcagc gctacctgtc caaggtgaag aagtcggtga accagaagtg 420gagggccaag agggagagct tgatgaggaa caagaggaag ggagcggagt cctgcaaggc 480gccgtcgtct ctggattgga ggaactacgg cattgtcacc ggcgtgaagg accagggaga 540atgcggaagt tgctgggcgt tctcttcgac cggagcaatg gaaggaatca atgcgctcaa 600gagcggggac ctgatcagcc tttccgagca agagctcgtg gactgcgaca ccaccaacga 660cggctgcgac ggcggctaca tggactatgc gttcgagtgg gtcatcaaca acggcggtat 720cgattcggaa gaagactatc cctacaccag cgtctttggc gagggtggta tctgcaacgt 780caccaaggag gagaacaaca aggcggtcac cattgatggg tatgtggacg tttatccgtc 840ggacgacggc cttctctgca cggtcatcca acagccgatt agcgtcggca tggacggctc 900ggcgatagat ttccagctct acactggggg catctacgac ggtagctgtt cagcgaatcc 960cgacgacatc gaccatgcgg tcctgatagt tggttatggc tccgaaggcg gcgaagatta 1020ttggatcgtg aaagaactcc ttggggaaca gatttgggga gt 1062
<210>110
<2l1>765
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>110caagctcaac ccaagttgag agaacatcat catgggttct tctccaagaa ctcatcatca 60actaggtctt gccatcctcg tctttgcttc cctcgcgcgc ctgtctctgt cacacgcctc 120tctccccagc gagtactcga tcatcggcca tggacaggac ccgaacggcg ccgtgtcgga 180cgaacgagcc gtcgagctct tccggagatg gcaggcgcag cacaagaagg tgtacaagca 240cgccggggag gccgagaggc ggctcgagaa cttcaaaagg aacctgaggt acgtgatgga 300gagaagccgg agggatggcg gcaagcagca tggcgtgggg ctgaacaagt tcgccgacct 360gagcaacgag gagttcaggc agctctacct gtccaaggtg aagaagtcgg tgaaccagaa 420gtggagggcc aagagggaga gcttgatgag gaacaagagg aagggagcgg agtcctgcaa 480ggcgccgtcg tctctggact ggaggaacta cggcattgtc accggcgtga aggaccaggg 540agaatgcgga agttgctggg cgttctcttc gaccggagca atggaaggaa tcaatgcgct 600caagagcggt gacctgatca gcctttccga gcaagagctc gtggactgcg acaccaccaa 660cgacgggtgc gacggcggct acatggacta tgccttcgag tgggtcatca acaacggcgg 720tatcgattcg gaagaagact atccctacac cagcgtcttt ggcga 765
<210>111
<211>677
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>111gagagagaga gagagaatgg ctggtgctag atttctgtgt tccttcctcc tcctcttgac 60cgcttgctcc gccacagcag ctggcttcca gggcgccgac ctcgagtcct ccatcctcca 120aaccgttggc cacggccgtc ccgccctctc cttcgtagac tttgccagca ggtacgagaa 180gaggtacgag acagcggagg agatcaagtt gaggttcgat aattacaggg agaatctcaa 240gctcattcga tccaccaacc agaagggctt gccttacact ctcgctgtta atcagtatgc 300tgactggagc tgggaggagt tcaagacgca cagactggga gcttctcaag actgctctgc 360caccaccaag ggcagccaca agctcaccga cgctgttctt cccaaaacga aagactggag 420aaaagagggc attgtaagcc cagttaaaaa tcaaggcggc tgtggatctt gctggagttt 480cagcgccact ggagctctcg aggctgctta tcaccaagca cacgggaaag gaatctctct 540gtctgagcag cagctcgtgg actgcgctac ggctttcaac aactttggat gcgatggcgg 600gttgccgtca caagccttcg agtacatcaa gtacaacggt ggccttgaga ccgaggaagc 660ttatccttat actgcac 677
<210>112
<211>522
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>112cacagtttgt ggaaaccaca gggagagaga gagagagaga gaatggctgg tgctagattt 60ctgtgttcct tcctcctcgt cgtgaccgct tgctccgccg cagcagctgg cttcgagggc 120gctgaccttg agtcctccat cctccaaacc gtcggccaca cccgtcccgc cctctccttc 180gtagacttcg cccgcgggca cgggaagact tacaagacag cggaggagat caagttgagg 240ttcgataatt acagggagaa tctcaagctc attcgatcca ccaaccagaa gggcttgcct 300tacactctcg ctgttaatca gtatgctgac tggagctggg aggagttcaa gacgcacaga 360ctgggagctt ctcaagactg ctctgccacc accaagggca gccacaagct caccgacgat 420gttcttcccg aaacgaaaga ctgggagaga aaagggcatt gtaggcccca gttaaagatc 480aaggcggctt gtggatcttg ctggagtttc agcgcaactg ga 522
<210>113
<211>392
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>113ctggattggg tggcaaaagg agccgtaaat gccataaaag atcaaggtcg atgtggctct 60tgctgggcct tctcagcggt agcggcaata gaatcgatta cccagatcaa gactggtaag 120ttactggaac tatcagagca acaattggta gactgtacta tcgaaaacta tggctgcagt 180gggggttgga tggacaccgc gttcgactac ataatacaaa atggaggcat ctcctctgaa 240actaattatc cctacaattc atcggacgga acatgcaatg ctcacatggc gtccctgagc 300gtggccaaaa ttgtgggtta tgaggatgtc cctgacaaca atgagggaga gatcttaaag 360gccgtggcga tgcaaccggt ctcggtcgcc ct 392
<210>114
<211>835
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>114tcttcttctg cgcactcttc accaaccatg aatcgcttcc tctctctcct cgctctcttc 60tccctcgcca tcgtctcggc ctacgcctcc tcggaggtcg acggcgatgc gctgatccgg 120caggtcgtgg acggcgccgc cgccgatggc gacctctcga ccgaggacca ccgccacttc 180tcgctcttca agaggcgttt cggcaagtcg tacgcctccc aggaggagca cgatcaccga 240ttcgcggtgt tcagggcgaa cctgcgccgc gcgaggaggc accaggagct cgatccctcg 300gcggtccacg gcgtcactcg gttctccgac ctgacgccct ccgagttcag gaggagtcat 360ttggggatca gaggcgggct ccggttgccg aaggacgcga atgaggcccc gctcctgccg 420accgacgacc tgcccgagga tttcgattgg agagatcacg gagctgtcac cggtgtcaaa 480aatcaaggct cgtgtgggtc atgctggagt ttcagcgcga caggagcact ggaaggcgcg 540cattaccttg ctactggaga actagttagc ctcagcgagc aacaacttgt ggattgtgat 600catgagtgtg atccagatga acccggttca tgtgactctg gatgcaatgg tggattgatg 660aacagtgctt ttgagtacac tcttaaagca ggtgggctta tgcgagaggg tgactacccc 720tacactggca ctgatcgcgg aacttgcaaa tttgacaagt ccaagattgc tgcatcagtg 780tccaacttca gcgttgtttc ccttaatgaa gatcaaattg cagcaaatct tgtga 835
<210>115
<211>291
<212>DNA
<213>辐射松(Pinus radiata)
<400>115cttcgctctc cgagcaagag ctcatcgact gtgacacaac ttacaacaat gggtgcaatg 60gcggtctcat ggactatgca ttctcttaca ttatctcaaa tggcgggctt cacaaggaag 120aagactatcc ttacatcatg gaagaaggaa cctgcgagat gaccaaggac caatcggagg 180tggtaactat cactggctac aaggatgtgc cggtggacaa tgagcagggc ctcttgaagg 240cactcgccaa ccagccactc agtgttgcca tcgaagcctc gggcagagac t 291
<210>116
<211>445
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>116aaaatgaccc tcgaggcggc ctcaaacctc ccgacatctt gttcaccccg attattgcag 60ttctccgacc tgaccccgtc ggagttccgg aggacgcacc tggggctccg gaggaaggtc 120aagctgccca aggacgcgaa cgaggcgccg atcttgccca cccaggatct gccgaaagat 180ttcgattgga gagatcatgg agccgtcacc gcggtcaaga atcagggttc atgcggatcg 240tgctggagtt tcagcaccac cggagccttg gaaggcgcaa actaccttgc aaccgggaaa 300cttgtcagcc tcagcgagca acagcttgtg gactgtgatc acgagtgtga tccagaagaa 360ccaggttcct gtgactcggg ttgcaatggt ggtttgatga acagtgcctt tgaatacact 420ctcagtaccg gtgtatgggt agtct 445
<210>117
<211>360
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>117ggcccaccag ggtcctgtcc tctgtggatg tgaaaccgtt caagtacgcg aacttcaccg 60ccattccagc tgccttggat tggcgaacca agaaggccgt gacgtctgtc aaggatcaag 120gcgtctgcgg atgttgttgg gcattctcgg cagtggcagc tatggaaggg cttacccagc 180tcaagaagag gaagttggtg ccactctcgg tgcaagaact tgtcgattgc gatgttaatg 240gtaaggataa aggctgcagg ggcggttaca tggacagtgc ctttgagttc gtaataagca 300acggcggcct cacgactgag gcagaatatc cttaccaggg aactgaccgg acctgcaaca 360
<210>118
<211>1428
<212>DNA
<213>辐射松(Pinus radiata)
<400>118aattgctcaa caatgggatc ctccacgctg ctgcttttgg ctctctgtat ttcctctgta 60atttgccttt cctcggccat aaggcctgac gatgacctta ttcgtcaagt gacggacgaa 120gtagattcag atccacagat cctcgatgct cggagcgccc tgttcaacgc tgaagcgcac 180ttcaggcgct tcatcaggcg ctacgggaag aagtactcgg ggccggaaga gcacgagcac 240cgcttcggtg tcttcaagag caatttacta agagccttgg agcaccagaa gctcgacccc 300caggcctccc atggcgtcac agaattctct gatttgacac aagaggagtt ccgacgacag 360tatctagggc tcagggcacc accgatccga gacgcccacg atgctccaat tttgcccaca 420aacgatctgc cggaggagtt cgattggaga gagaagggag ccgtgaccga ggttaagaat 480cagggatcgt gcggttcctg ctgggctttc agcacaaccg gggcgctaga gggcgcgaat 540ttcctgaaga cggggaagct ggtgagcctg agcgagcaac aattggtgga ctgcgatcac 600gagtgcgatc cttcggacgc aagatcatgt gattctggtt gcaatggtgg gctaatgacg 660agtgcttatc aatatgctct gaaagctggt ggattggaga aggaagagga ctacccatat 720actggaaaag acggaacttg cagctttaac aagaacaaaa ttgtggcaca ggtttcgaat 780ttcagcgttg tttctattga tgaagatcaa attgctgcaa atctggtgaa gaatggacct 840ctatcagtgg gaatcaatgc tgcatttatg cagacatacg taggaggtgt atcttgccca 900tacatctgca gcaagcgcat gttggatcat ggtgtgctcc tggtaggata tggttctgca 960ggctttgctc ccattagaat gaaggacaaa ccctactgga tcataaagaa ctcatgggga 1020cctaactggg gagaaaatgg attctacaaa ctttgcaggg gacataacgt ttgcggaatc 1080aacaacatgg tttccactgt tgcagctatc tgagcattca aattagatca gtttcttgta 1140tatacctgtt tctttagact ttggttgaaa ctatgttgtt gaatgcacat acatattcaa 1200tatacattga atatagttta ttcaagtata ctgaaggatt taagtattta aaggatttac 1260aaatgaagca ctctgatgaa tactcttaag aatattaata tggtttgtgg ttcaaaaaac 1320tgccagtcaa gggcttcaaa ctctggtttc caagtttgtt tgaggtgatg taaatatggg 1380agtttatgat ttgtcataat atgggaaatt gtcgaatcaa aaaaaaaa 1428
<210>119
<211>413
<212>DNA
<213>辐射松(Pinus radiata)
<400>119ctcaacaatg ggatcctcca cgctgctgct tttggctctc tgtatttcct ctgtaatttg 60cctttcctcg gccataaggc ctgacgatga ccttattcgt caagtgacgg acgaagtaga 120ttcagaccca cagatcctcg atgctcggag cgccctgttc aacgctgaag cgcacttcag 180gcgcttcatc aggcgctacg ataagaagta ctcggggccg gaagagcacg agcatcgctt 240cggtgtcttc aagagcaatt tactaagagc cttggagcac cagaagctcg acccccaggc 300ctcccatggc gtcacagaat tctctgattt gacacaagag gagttccgac gacagtatct 360agggctcagg gcaccaccga tccgagacgc ccacgatgct ccaattttgc cca 413
<210>120
<211>475
<212>DNA
<213>辐射松(Pinus radiata)
<400>120caatcccaag tctctagagt ttgcggagtt cgctgtcaga tatggcaaga ggtacgattc 60tgtccatcag cttgtgcata gattcaatgt ctttgtgaag aacgtggagc tgatcgagtc 120aagaaacaga atgaagcttc cttatacttt ggcaataaat gagtttgctg acataacatg 180ggaggaattc catggacaat atttgggtgc ttcacagaac tgttcggcta cccacagtaa 240ccataagttg acgtatgccc agcttcctgc gaagaaagac tggagacaag aaggcatagt 300gagtcctgta aaaaaccaag cccattgtgg atcctgctgg acattcagca ctactggagc 360actagaagct gcctatactc aggctacagg aaagactgtt atcctgtctg aacagcagct 420ggttgactgt gctggagcat ttaacaactt tggttgcaat ggtggactgc catcc 475
<210>121
<211>399
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>121cagcggcgga gagatggaca ccgcgttttc cttcatccaa cgcaacggcg gcatcacttc 60cgagtccgat tatccctacc gaggacggga cggatcttgc gacgcggcca tgctcaggag 120tcacgcggcc acgattagcg gatacggcga tgtgcccccc aacgatgagc ggagcctgca 180agccgcggtg gctcgccaac cgatctccgt ggccatcgac gcgggcggac tggaattcca 240gctatactcc agggggattt tcacgggcat atgcggatac gacttgaatc acggggtggc 300ggcggtcggg tacggatccg agggctcgag aaattattgg atcgtgaaga attcgtgggg 360gcgcgactgg ggcgaggacg gctacgtaag gatgctcaa 399
<210>122
<211>441
<212>DNA
<213>辐射松(Pinus radiata)
<400>122acatacaagc ttggattgaa taaatttgca gatctgagta acgaagaatt caaagccatg 60catatgacaa ccacgatgga gaaccacagg agtctgcgca gagatagagg agtgcagagt 120ggatcattca tgtatcaaaa ttcgaagcat ttgccagcat ctattgattg gagaaaaaag 180ggagctgtta ctccggtgaa gagtcaaggc caatgcggaa gttgttgggc attttcaact 240gtagcttcgg tagagggcat taactatatc aaaacaggga agctggtttc tttatcagag 300cacaggttgg tagattgcag taaagagaac gcaggttgca atggaggact tatggataac 360gccttccaat atatcataga taatggcggg atcgttagtg aagcagagta tccttacact 420gccgaagcga gcgagtgcag t 441
<210>123
<211>370
<212>DNA
<213>辐射松(Pinus radiata)
<400>123gatggcgagt gtttccaagg caaccctgtt actcttcctt gcgaccacct tgtggacttt 60atcagcccat gcttcggatt cttcccctgg atttacagat gaagacctca agtctgagga 120aagtctgcga ctcctttatg acaaatgggc acttcggcat cgcaccacca gaagtttgga 180ttcggatgag catgccaaac gattcgagat attcaaagac aatgtgaaat atatcgactc 240cgtgaatcag aaggatggtc catacaaact tggattgaat aagtttacag atctgagtaa 300cgaagaattc aaggccatgc acatgacaac tagaatggag aagcacaaga gtctgcgccg 360agacagagga 370
<210>124
<211>370
<212>DNA
<213>辐射松(Pinus radiata)
<400>124gatggcgagt gtttccaagg caaccctgtt actcttcctt gcgaccacct tgtggacttt 60atcagcccat gcttcggatt cttcccctgg atttacagat gaagacctca agtctgagga 120aagtctgcga ctcctttatg acaaatgggc acttcggcat cgcaccacca gaagtttgga 180ttcggatgag catgccaaac gattcgagat attcaaagac aatgtgaaat atatcgactc 240cgtgaatcag aaggatggtc catacaaact tggattgaat aagtttacag atctgagtaa 300cgaagaattc aaggccatgc acatgacaac tagaatggag aagcacaaga gtctgcgccg 360agacagagga 370
<210>125
<211>392
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>125gcaaactata agaatccatc caccccccct cctctctctc tatctctaac aaacttctgc 60accagaagct cgacccctcg gccgcccacg gcgtgacgca gttctccgac ctgaccccgt 120cggagttccg gaggacgcac ctggggctcc ggaggaaggt caagctgccc aaggacgcga 180acgaggcgcc gatcttgccc acccaggatc tgccgaaaga tttcgattgg agagatcatg 240gagccgtcac cgcggtcaag aatcagggtt catgcggatc gtgctggagt ttcagcacca 300ccggagcctt ggaaggcgca aactaccttg caaccgggaa acttgtcagc ctcagcgagc 360aacagcttgt ggactgtgat cacgagtgtg at 392
<210>126
<211>452
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>126ggcacgagct taggcaactc attgttcatt gttaaccttc tctcttcctt gagaggaacc 60ccaaaggagt tacgaagtaa tggcttcttc ttcctccaag gggcccaaaa ggagttacga 120tgcaatggct tcttcttcct ccaagaagcc cagaagaagt tacgatgtct tcctgagttt 180cagaggtcca gatgtccgca atcactttct cagtcatctc tacgtagctc tagatcaagc 240agggatatcc acttacatcg acaaaaaaga gctggggaag ggagaacaaa tatcacctgc 300acttatgaaa gcgatcgagg aatcgcacat cgcgatcgtg gttttctctg aggactacgc 360ctcttcgtca tggtgtttgg aagagttaac gaaaatcatg gagtgcaagg agcaaaaagg 420cctcatggtc tttccagtgt tttacaaagt ag 452
<210>127
<211>452
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>127gcaactcatt gttcattgtt aaccttctct cttccttgag aggaacccca aaggagttac 60gaagtaatgg cttcttctcc ctccaaggag cccaaaagga gttacgatgt cttcctgagt 120ttcagaggtc cagatgtccg caatcacttt ctcagtcatc tctacgcagc tctagatcaa 180gtagggatat ccacttacat cgacaatgaa gagctgagga agggagaaca aatatcacct 240gcacttatga aagcgattga ggaatcgcaa attgcaattg tggttttctc tgagaactac 300gcctcttcaa catggtgttt ggaagagata tcgaaaatca tggagtgcaa ggagaaaaaa 360ggcctcaagg tccttccggt gttttacaaa gtagaaccaa gagaagtgag agggcagaaa 420cagagctatg gaaaagctat ggatgagcat ga 452
<210>128
<211>3095
<212>DNA
<213>辐射松(Pinus radiata)
<400>128agctggagct cgcgcgcctg caggtcgaca ctagtggatc caaagaattc ggcacgaggc 60tgttttgttt tcaatcgtgt gcccaaacat tggggtgttg atacacacac aagaatagca 120aaggcgcagc tggtaagttg cttgccctcc agaatctttc tgacgcaaat attgagtctc 180accgcacttt ctccatctct gcaaaatatc ttcgatctta cttctctgtt attctgtatc 240caatggcaaa cgttaacttt acccccgccg cccgtgacgg aacatcctcc gcatccacct 300cccagggcaa taccaacagc tatgtgtatc aagtgtttct gaatcaccgc ggtcctgacg 360taaagaaagg actcgctacc cacatctacc atcgtcttaa agatctcgga ttatcagttt 420ttctggacca gcaagaactg caaagaggag agaagttgga gccccaaatc gaaggggcta 480ttcgaacagc ttctgttcat gtagcgatat tctcgccaaa ttatgctcaa tctagatggt 540gtctggatga actcgtccag atgttggaga tgttggagtc agggtccaca ataattccag 600tcttctacaa agtagatccc gcagatctcc ggtggacgcg cggaggaaaa ggagtttatg 660ctagagattt gggcgagctt gaaaggaaga gagcatctga ttctcaggaa ccacggtacg 720accccgagac catagaaaaa tggaggaatg ctctttctgc tgtggcggat atagtcggtt 780ttgagctgaa ggacaaggaa gagtcgcagc tcgtccagga ggtcgtccaa caagttgtga 840aaaaggttcg caaaccgcct ctcaacgtcg ccaaatatcc tactggcctc gatgaaaaaa 900ttgaagatgt ggacagaaca ttgtcactgc agcggcaaag cgagaaggct acaattttgg 960gaattgtagg ttttggcggg gtcgggaagt ctaccctggc taaacaattc ttcaaccgag 1020aaagatcaaa ttacgatcga tcttgcttct tatctgatat cagatccaaa tctttgcctt 1080ccgtgcagag tagccttctt aaggatctga ttcaatcgga tgcacaaata aatagcgttg 1140ctgaaggcat agagaagctc aaaagagttt ctcaacggtg tcttatcatt ttagatgata 1200ttgatcatat tgatcaaatg gatgcattat atgcacccgt tataaggtct attcatgtag 1260gtagcttaat tttaattaca tcccgtaata aggatgtgct tagaagcgca ggcattggag 1320agtcatccat ttgcacactg aaaggtctca atggagagca ctcgcaagag ctcttttgct 1380ggcatgcctt cggtcgaccc agtcctgttg taggatttga aaaagtggtt gagaagtttt 1440tgaatgcctg caatggtttg cccctctcgc ttaaagtgct tggggcgctc cttcatggaa 1500aagacgattt gaagctttgg aatgcgcaat tgcgcaaaac ctccaaagta cttccggaag 1560atatacggag cacacttaga attagctatg atgctctaga taaagaggaa aagcaaatat 1620ttttagatat cgcctgtttc tttataggga aaaacaggga tagtgctata agagtatggg 1680atggatccaa ctgggaaggg ttgctgggtc tttggaagct ggaaaacaga tgcctcgtgg 1740aggtggacag ttcgaactgt ctcagaatgc atgaccacct tcgagacata ggcagaggta 1800tagctgaata tctagaatat ccccgtcgtc tttggcattt cgaagagaat tttcttgtaa 1860gtattctgaa gctttcgacg gactttcaaa atagcccctt gttatcaaaa ttgtctgtca 1920atttctaaat cttaaaacta acagcttggg gtgaacaatt cagatgcagg tgcatggaat 1980tagcaggtcc aaccgaagca tgttgcagct gcttaggact gaaagtgact ctgttgaacg 2040aatattcagc agtgaacagt taccgccgct tgtatatctg cactggaagc gctgtccgaa 2100gtcctcttta gctccctgga ttccattaaa taatttaagt gtgttacata tagaggggga 2160tcaactggaa acactctcac tctggcaaca tgaatttcag gcacctctgc aattgaggga 2220gttgcatatt gatgctcccc tttcaaaggt tccagagtcc attggaaaac tgaagtacct 2280tgaaaaagtt catctggaga gcaagcagct gcagacgcta ccagagtcga ttgggaacct 2340gtcgggcctc caaagtcttg acctgatcgg gtgctccagt ctgcagacac tcccagactc 2400agtggggaac ctgtcgggcc tccaaagtct taaattggtc ttctgcttca gtctgcagac 2460actcccagac tcagtgggga acctgtcggg cctccaaagt cttgacttga tcgggtgctc 2520cagtctgcag acactcccag actcagtggg gaacctgtcg ggcctccaaa gtcttaactt 2580gaacatgtgc aggagtctgc agacactccc agactcagtg ggtaacctgt cgggcctcca 2640aagtcttaac ttgagcgggt gcaggagtct gcagacactc ccagactcaa tggggaacct 2700gtcgggcctc caaagtcttg acttgagctg gtgcaaaaaa ctgcggacac tcccacactc 2760actggagaac ctgtcgggcc tccaacgtcg cgacgatgat tatttgaatt ttgatcacaa 2820aatccacaaa atctgataag tgattttatt gggagttgtc tataatgcga ctttttggag 2880attccggtga cggaatccgg cggatgtaat gaggttaaat cttgattttt agcagttaaa 2940atggtttttt aactggattc agcgccctga aaaacccctg ggagcctttc attgtgcaga 3000aactagctat caaacttggg attccaatga attgctgccg gatttttacc ggaaaaatcg 3060gccaaaaaga ttttcaaaaa aaaaaaaaaa aaaaa 3095
<210>129
<211>543
<212>DNA
<213>辐射松(Pinus radiata)
<400>129gcatctgatt ctcagaaatc acggtacgac accgatacca tagaaaaatg gaggaatgct 60ctttcttctg tggcggatat agtcggtttt gagctgaaag acaaggaaga gtcgcagctc 120gtccaggagg tcgtccaaca agttgtgaaa aagtttccca aaccgcctct cgacgtcgcc 180aaatatccta ctggcctcga cgaaaaaatt aaagatgtgg acagaacatt gtcactgcag 240cggcaaagcg agaaggctac aattttggga attgtaggtt ttggcggggt cgggaagtct 300accctggcta aacaattctt caaccgagaa agatcaaatt acgatcgatc ttgcttctta 360tttgatatca gatccaaatc tttgccttcc gtgcagagta gccttctcac ggatctgatt 420caaccgaatg cacaaataaa caacgttgat gaaggcatag agaggctcaa aacagtttct 480caacggtgtc ttatcatttt agatgatatt gatcatattg atcaaatgga tgcattatat 540gca 543
<210>130
<211>397
<212>DNA
<213>辐射松(Pinus radiata)
<400>130attgatcaac tggatgcatt atgtgcaccc gttatagata ctattgatgt aggtagctta 60attttaatta catctcggaa taaggatgta cttagaagcg caggtattgg agagtcatcc 120atttacacac tgaaaggtct caatggaaag cactcgcaag agctcttttg ctggcatgcc 180ttcggtcaac ccagtcctgt tgtaggattt gaaaaagtgg ttgagaagtt tttgaatgtc 240tgccatggtt tgcccctctc gcttaaagtg tttggggcgc ttcttcgtgg aaaagacgat 300ttggagcttt ggaatgcgga attgcgcaaa acctccaaag tacttccgaa agatatacgg 360agcacactta gaattagcta tgatgctcta gataagc 397
<210>131
<211>3124
<212>DNA
<213>辐射松(Pinus radiata)
<400>131ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagccgc aattacttgt 60gagttagctc actcattagg caccccaggc tttacacttt atacttccgg ctcgtatatt 120gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc ttgattacgc 180caagctcgaa attaaccctc actaaaggga acaaaagctg gagctcgcgc gcctgcaggt 240cgacactagt ggatccaaag aattcggcac gagacccaat cttctacaat ttctctgcgc 300ttttaaaaca gtgaatcgtc ccgttgcaga ctctgtgtct atagcatcca accattacta 360ccttaatttc tgttttgatc tcggcaatgg catcatcttc atcctccacc ttctccagca 420acaaatccca ccaagttttc ataaaccatc gcggggttga tgtgaagaaa accttcgcca 480ggtctctata ccttcgcctt cgcgagaaag gattgacggc tttcctcgat gacgaagaga 540tgcaagtcgg atatgaaatt tctcctcaac tcgcagacgc tattaggacg gcctccgttc 600atgtggccat cttctctccg agatacgctg aatcagaatg gtgcctcaat gagctccttg 660agatgttaaa gtcaaagaaa cctataattc ctgtatttta cggcatcagt ccggctgagg 720ttcggtggat gcagggcgtc tatggtaaag ctctacaaac tcataaagag aagccgcgaa 780atgatcccag cactattgaa gaatggagaa atgcactcca tcaagttgca aacaggagcg 840ggttcgagct ggacaagtac cctgggtaaa aactcaaacc ttcctctggc tgtattttat 900attggataaa taggcataga cgtttgtttc tcactaggtc tacttaattt ctccagccag 960gatttggtgc tggaactgct agataaggtg gttaaacatc tgttggaatt ggtgccaaag 1020ccagatttgt acgtggcgga gtatccaacc ggcctcgatg acaaactgaa agattttgaa 1080gatacagtcc tgttgaggca acaacagggc cgaaaacccc agatcttagg gattgtgggg 1140ttaggtggtg tgggaaagac aacacttgcc acagcattct tcaataagaa gaaatcagct 1200tatcatagaa gttgtttttt atgtgatgtc agagaaaata caaccaatag gtctttacat 1260ttgttgcaga gtcaacttct taatagcttg actggcttca ataatcaggt aaacagtgag 1320cgtgaaggta aggggatgct tatagagcct ctcaaatctt gtaaagccat aatgatcttt 1380gatgatgttg acgatgtgga tcaggtgaag gcatttttgc cccaatctga tgtactcaat 1440tcagagagct taatattgat tacaactaga gataggaatg ttttgagaag cttaaaagtt 1500gaaaattcat caatttatag cctatcagga cttaataaag agcactccct agaactcttt 1560tgttcccatg ccttcagccc agcatttcct ctcccagaat ttaaatccct ggtagataag 1620ttcatagatt attgtaatgg attgcccctg tctttaaaaa tatttggggc acttctttat 1680ggtaaagata tatctcagtg gaaagaagaa tgggagagtc ttagacaaat agctcccatt 1740gccatacatg acacatttaa aataagctat gactccctca atcaagagga aaaagatata 1800ttcttagaca ttgcatgctt tttgcgatgt caccacagag atgccgcaat aagcatatgg 1860aacaaatcgg gctggagagg aaatcggggg tttctaaatc tacaggacaa atctcttgtg 1920gaagttgacg cctttaattg tatacagatg cacaaccacc taagggacct gggaagacag 1980gttgcagcgt cttcgttgcc tcctcgtctt ttaataacaa agaacctcat ccataatttg 2040tcacaccaat catctgtaag tgttcagtca ttcaatcctt tgtttgttat tcactaaaag 2100tcagagattg tttgttgaat gaagaagtaa taacgattgt ttttcaacaa tggacaggaa 2160ataaccttcc gcggaattgg gatggttccc ggtgaatata gtgcccaaga tgatgatgat 2220gatgatgtat ttggcgtcgt tgatactgaa aatgctgtct tggaacgcag tttcaggagt 2280gtgcaaattc ttgaaatgga aggttgtcac ctggaaccca ttttgaggaa ggcaaagcca 2340ccaaacctat catgcctcag ctggaacaga tgtcctcact cttccctgcc ttcctgggtt 2400tcagtgaaga atttaaggat tctacatctg gagcaatgcg aactaaaggc attgtggcct 2460tcccagtttt cagtgaagaa tttatgggct ctattgtggc actggaaaag tgaatcacag 2520tcacctctgc agtttccgat atctttggag cagctggtgg tacgccaatg tgagaaattg 2580agaagcataa ccgggttggt gcatgcgaca aatcttcggg agctaaatgt ttctaactgc 2640tctgagttag aagaactgtc tagtttggaa gcattggtat cgttggagca tttgcaggct 2700gatggatgta agaaactgaa agccatacga gggttggtgc atgcgacaag gcttagagta 2760ctggatgttt ctaactgctc tgagttagaa gaacttccta gtttggaaac attggtatcg 2820ttggagggtt tgtgggctga tggatgtaag aaactgaaag gcatacgagg tttagcccac 2880gccacaaatc ttcggacgct aagtgttcgt gagtgctttg ccttggaaga attcagagat 2940gtctctgaat gtcacaaatt gagtcacaaa ttggtgtggg aatagcggag cagttgtttg 3000ggaatagtcg atgtctctga atgtcacaaa ttggtcctcc tgtccatcat gtatagtggt 3060gtcatttaaa cacaactcca caaaagaatt tataaattat ttgtactaaa aaaaaaaaaa 3120aaaa 3124
<210>132
<211>1653
<212>DNA
<213>辐射松(Pinus radiata)
<400>132agacgaaaat attgaggttc accgctcatt cccaatctct gtaaaacatc ttggatctcg 60cttgcttctt tcttctctgt aaaacgtctt ggatctcgct tgcttctttc ttcttctgtg 120tccgatggcg gaccataccg gggatatcac atgcatcgcc tcttcttcgt cttcatccac 180caacactggc caggtttttg acgttttcct caaccaccgc ggtcccgaca caaagaaagg 240cctcgccagc catatctacc gtggccttat tgtccgtggg ttaagagtat ttctcgacca 300gcccgagttg cgtaagggag aggacaacct ttctcaaatc aaagaggcta ttcgaaccgc 360ctcagtgcat gttgcaatat tttctccaaa ctatgctcaa tcaagatggt gtcttgatga 420gctggcacta atggtggaat ccgagtcgac aataattcca gtcttccatg atgtcgatcc 480ctccgaactt cggtggcagc agagtggaga tggagtcgag agtatcatcc gctgtctctg 540tccgtgtcta ctgggcggaa agggacggta tgctcgagac ctgcacatgc tccaaaagaa 600gacaacattg gatcctcata ccaacaaaaa gaagccacga catgactcga gaactctgca 660aaaatggagg aaagctctct ctgatgtctc caataaaagc ggttttatta tcaacgcata 720caatggtgac gaagggcagc tagttgatgc ggtcgtcgaa gaagtgtggc gaaaggttga 780gaaaacacct cttaatgtgg ccaaataccc tactggcctc gttgagaaaa tagaagacgt 840gggaagaatg gtactcttgc agcatcaaag ccagaagact aaggttgtgg gaattgtggg 900tcttggcggt gtagggaaaa cgacccttgc aaaagaattc ttcaacagac acagatcaaa 960ttatgatcga tcttgttttc tgtttgatgt gagagaaacc gcagccaaga gctctttaag 1020ttcattgcag acacaacttc ttaaacactt ggctcatttg caggacgagc aaataagaaa 1080caccgatgaa gtcatagaga agctccgaaa gcatctctca tcctcccccc gatctctgat 1140agtcttagat gatgttgatc atattgatca acttgacgca ttgttttcac cggtgataga 1200taccattcag gccagtagtt taatcttagt tacttctcgc aatagggatg tacttataag 1260ctcgggaatt ctagaggcgt ctatttacca gcaaacaggt cttaacccac aacagtccag 1320agaactattc tgctcgcatg cctttgacca gagttgtcct gtgacgggat ttgaacaact 1380ggttgaagat tttttggatt tctgtgatgg attgccctta tctcttaagg tcatcggagc 1440ggctattcgt ggaaaagatt ctgagttttg gataggacag ctagacaaaa acagaagaat 1500acttcacacg gatattcata gcaagcttaa aattagctat gatggtctgg ataaagagga 1560gcagcagatc tttttagacg tcgcttgttt ttttatagga gaaaacagag ataccgccat 1620cagaagatgg aatggatctg acggtaagtg tac 1653
<210>133
<211>346
<212>DNA
<213>辐射松(Pinus radiata)
<400>133cagccatatc taccgtggcc ttattgtccg tgggttaaga gtatttctgg accagcccga 60gttgcgtaag ggaaaggaca tcccttctca aatcaaagag gctattcgaa ccgactcagt 120gcatgttgca atattttctc caacatatgc tcaatcaaga tggtgtcttg atgagctcgc 180actaatggtg gaatccaagt cgacaattat tccagtcttc catgatgtcg atcccttcga 240acttcggtgg ccgcagagtg gagatggagt cgagagtatc atccgctgtc tctgtccgtg 300tctactgggc ggaaagggac ggtatgctcg agacctgcac atgctc 346
<210>134
<211>1737
<212>DNA
<213>辐射松(Pinus radiata)
<400>134cttgtagaga tcgacagtga agggtgtata agaatgcacg accacctgcg cgatctcggc 60agagatgtag cagaaaagga acatccgctt cggctttcgc gaccaaatgt caatcttctt 120cgtactcttt ccccctcatc gcctgtgcga ggaattagca tgaattatgg aaatggcggc 180aaacaatttt tggaatacat tagggtcaac tgcaacctaa gcaggttgga actgcttagg 240ggtgaaggtt cttttgttga aagcatattc agtgcagggg agatacgaca actggtatat 300ctccaatgga aggagtgtcc gatctcctca atatctttca caatcccaac aaggaattta 360agcgtgttat acatacaggg ttatgctttg aaaacactct ggcaacatga atctcaggca 420ccactacagt tgacggaatt gtatattgat gctacccttt cagaggttcc acagtctatt 480ggtaaactga atcagctcga aagaattgtc ctgaaaaatg gttattttaa aactttacca 540aatgaattct atgatatgca ttcattgaag catatcacgt tacaaaactg tgaacaaatg 600atgttgctgc cggattcagt tgggatcttg acgggccgcc aaacgcacga cttttccggg 660tgctccaacc tgcaagcgct cccagactcg gtggggcagc tgacgggcct caagacgctc 720gacttggaag actgcaccag cctgcagggg ctcccagact cggtggggca gctgacgggc 780ctccagagcc tcgacttgga acactgcacc agcctgcagg ggctcccaga ctcggtgggg 840cagctgacgg gcctccagac actcgacttg cgtgggtgct ccagcctgca ggggctgcca 900gactcggtgg ggcagctgac gggcctcgag ggactctact tgagcgggtg cttcagcctg 960caagggctcc cagactcggt ggagcagctg acgggcctcg agggactcta cttgagcggg 1020tgcttcagcc tgcaagggct cccagactcg gtggggcagc tgacgggcct ccagagcctc 1080aacttggaat actgcaccag cctggagggg ctcccagact cggtggggca gctgacggac 1140ctcccgatac tcgacttgaa tacgtgcatc agcctgcagg ggcttccaga ctcggtgggg 1200cagctgaggg gcctccagaa cctcgacttg cgttggtgcg acagcctgca ggggctccca 1260gactcggtgg ggcaactgac gggcctccag atactcgact tgagtgggtg caccagcttg 1320caggggctcc cagactccgt ggggcagctg acgggcctcc ggacgctcca cttggaaaac 1380tgcaccagcc tgcaggggct cccagactca gtcgggaact taacgagtct caaatggctt 1440aacttatctg ggtgttccaa tttacagatg ctgcccaatt tccgtcattt gagctcgttg 1500gaggagcttc acttgtctgg atgttccaat ttacagatgc cgcccaatgt tcagcatttg 1560agctcgctgg tggagctttc tgtgtctcac tgttcaaaac tgcaatgggg tgctggagta 1620gtcgagtccc tgcgccatcg actgggaaat ggcttcatcg aagaaggcgg cgaaaacatt 1680gataaagaaa gttgggaaga aggcagcgaa aaaagtgata aagaaagttg ggaagaa 1737
<210>135
<211>527
<212>DNA
<213>辐射松(Pinus radiata)
<400>135cggtcctcca gacactcgac ttgcgtaggt gctccagcct gcaggggctg ccagagtcgg 60tggggcagct gacgggcctc cagagcctca acttggaaaa gtgcaccaga ctacaggggc 120tgccagagtc ggtggggcag ctgacgggcc tccagacact cgacttgcgt aggtgctcca 180gcctacaggg gctgccagag tcggtggggc agctgacggg cctccagagc ctcaacttga 240aagagtgcac tagcttgcag gggctcccaa actctctggg gcagctgacg ggcctccata 300gcctctactt ggttgagtgc tccagcctgc aggggctccc agactcggtg gggcagctga 360cgggcctcca gagcatcaac ttgcaaggct gctccagcct gcaggggctc ccagattcgg 420tggggcagct gacgggcctc cacagcctca acttagaagg ctgctccaga ctgcaggggc 480tcccagactt ggtggggcag ctgacaggcc tccagagcct caaattg 527
<210>136
<211>485
<212>DNA
<213>辐射松(Pinus radiata)
<400>136tggaccgctg ctccagcctg caggggctcc cagactcggt ggggcagctg acgggcctcc 60ggcaactcaa cttgaacggg tgctccagcc tgcaggggct cccagactcg gtggggcagc 120tgacgggcct ctggatactc gacctgaccg ggtgctccag cctgcagggg ctcccagact 180cggtgaggca gctgaggtgc ctccggggcg gaagcggacg ggcctgcgga cgggcagcgg 240aagcaccagc ctacaatggc gggctgccag gcgggctcgc agaatcacga ttagcgagag 300gctattggct taacttgtct gggtgttcca atttacagat gccgcccaat gttcagcatt 360tgagctcgct gttgaagctt tatgtgtctc actgttcaaa actgcaatgg ggtgctggag 420tagtcgagtc cctgcgccat cgactggaaa taacttcgtc gaagaaggcg gcgaaaacat 480caatg 485
<210>137
<211>918
<212>DNA
<213>辐射松(Pinus radiata)
<400>137ggggctccca gactcggtgg ggcagctgac gggcctccag acactcgact tgcgtgggtg 60ctccagcctg caggggctgc cagactcggt ggggcagctg acgggcctcg agggactcta 120cttgagcggg tgcttcagcc tgcaagggct cccagactcg gtggagcagc tgacgggcct 180cgagggactc tacttgagcg ggtgcttcag cctgcaaggg ctcccagact cggtggggca 240gctgacgggc ctccagagcc tcaacttgga atactgcacc agcctggagg ggctcccaga 300ctcggtgggg cagctgacgg acctcccgat actcgacttg aatacgtgca tcagcctgca 360ggggcttcca gactcggtgg ggcagctgag gggcctccag aacctcgact tgcgttggtg 420cgacagcctg caggggctcc cagactcggt ggggcaactg acgggcctcc agatactcga 480cttgagtggg tgcaccagct tgcaggggct cccagactcc gtggggcagc tgacgggcct 540ccggacgctc cacttggaaa actgcaccag cctgcagggg ctcccagact cagtcgggaa 600cttaacgagt ctcaaatggc ttaacttatc tgggtgttcc aatttacaga tgctgcccaa 660tttccgtcat ttgagctcgt tggaggagct tcacttgtct ggatgttcca atttacagat 720gccgcccaat gttcagcatt tgagctcgct ggtggagctt tctgtgtctc actgttcaaa 780actgcaatgg ggtgctggag tagtcgagtc cctgcgccat cgactgggaa atggcttcat 840cgaagaaggc ggcgaaaaca ttgataaaga aagttgggaa gaaggcagcg aaaaaagtga 900taaagaaagt tgggaaga 918
<210>138
<211>257
<212>DNA
<213>辐射松(Pinus radiata)
<400>138atgctgcccc attttcggca tttgagcttg atggaggagc ttcacttgtc tggatgttcc 60aatttacaga tgccgcccaa tgttcagcat ttgagctcgc tggtgaagct ttatgtgtct 120cactgttcaa aactgcaatg gggtgctgga gtagtcgagt ccctgcgcca tcgactggga 180aatggcttca tcgaagaagg cggagaaaac agtaatgaat acaactgcag cgagttgtat 240aatataagag aacttct 257
<210>139
<211>1166
<212>DNA
<213>辐射松(Pinus radiata)
<400>139tgaacagctg caccagcctg caggggctcc cagactcggt ggggcagctg acgggcctcc 60ggacactcga cttgaacagc tgcaccagcc tgcaggggct cccagactcg gtggggcagc 120tgacgggcct ccggacactc gacttgcaca gctgcaccag cctgcagggg ctcccagact 180cggtggggca gctgacgggc ctcgagacac tcgacttgca agactgcacc agcctgcagg 240ggctcccaga ctcggtgggg cagctgacgg gcctccaggt actctacttg agacggtgct 300ccaacctgca ggggctccca gactcggtgg ggcagctgac gtgcctcaag gtactgtgct 360tgagatggtg ctccaacctg caagcgctcc cagactcggt ggggcagcta acgggcctca 420aggcactcaa cttgcaagac tgcaccagcc tgcaggggct cccagacttg gtggggcagc 480tgacgggcct caaggcactc aacttgcaaa actgcaccag cctgcagggg ctcccagact 540cggtggggca gctgaccggt ctccaggtac tctacttgag acagtgctcc aacctgcaag 600cgctcccaga ctcagtgggg cagctgacgg gcctgaataa actctacttg aacgggtgct 660ccagcctaca ggggctccca gactcagtcg agaacttaac gagactcaaa tggcttatat 720tgtctgggtg ttccaattta cagatgctgc ccaattttcg gcatttgagg tcgttggaga 780ggcttcactt gtctggatgt tccaatttac agatgccgcc caatgttcag catttgagct 840cgctggtgca gctttatgtg tctcactgtt caaaactgca atggggtgct ggagtagtcg 900agtccctgcg ccatcgactg ggaaatggct tcatcgaaga aggcggcgaa aacagtaatg 960aataaagctg cagctagttg tataatataa gagaacttct taagatcaga tggccacggt 1020gctcattaat cttcatagag ctgtaatcag aggcaaaatg aattacacaa tgtacagaat 1080gctctacatt ttaaaaaaaa aaaaaaaaaa aaaactcgag agtacttcta gagcggccgc 1140gggcccatcg attttccacc ccaggt 1166
<210>140
<211>1921
<212>DNA
<213>辐射松(Pinus radiata)
<400>140cttgcattat aggttgttct aaagtttctg aaactggttt gcaatttaca tcaatcattg 60aaagctatcc atcgcttatt cggcaatggc atccaccgcc tccactgcca tcaacacaag 120caattatcaa tacgacgtgt ttctgaatca tcgcggccgc gatacgaaga acgacttcgc 180cggtcatctc tactctcgcc ttcgttcacg tggaatccgt gtctttctgg acaaggaaga 240gatgcaagtg ggagacgatt taacttatca gataaaagcc gttattaaaa ccgcctctgt 300ccatctggcc attttcagtg agaactacgc cgagtcaaag tggtgccttg atgagcttgt 360gctgatggtc gagtcggagg ctaccatcat ccccgtcttt tttaaaaatg tggaacctga 420tcatcttcag tggattggaa gccagactcg ggataccgag cagagcgggg ataccaagca 480gagcggaagc cagactcggg aaaccgagca gagcggaagc caagcaaaca agaagccaca 540gaagacaaag tttgctgatg gcttctggaa acttcaaaat aagacggata atgggcagcg 600gcgacacaaa catgagacta ttggaaaatg ggcatctgct ctttcaacag tttccgaaag 660aacctgtttc aagctggcag cgtttaatgg gtgagaactc aatttccctt ttcagtatgc 720tacatagatg taaatggacg tttctcactt gttctattta ctctcttgtg aataaccagc 780gacgaggtgg agctaatcga gaagatcgtc cagtctgtgt tagaaaaagt gaatagatct 840tccttctatg tgcccaaata tgaggtcggt ttggatgaga atgtagaaaa gtttcgtaaa 900aaggtaaaag aatggtcgca gcagcggcag aatgaaaaag ctcaggtcgt ggggatagtg 960gggttgggtg atgttggaaa gacaacgcta gtaaaggaat tcttccctac agagagtcca 1020gcataccgta acttctgctt ttaccctgtg aggcgaaatg gatgtataac acgtccagat 1080tgtttgattg gagagctttt taagggctcg agtggtttgg tttcgtcacc aaccagcgtt 1140gatgcagtta aaatattgcc ggacgcgtct aatccctcag tagacatgat tcgaaacaat 1200ccctccttag tagttttaga tggagtagat aatgttgagg agagggagaa tcttttgaaa 1260attcaagaaa ggctccactc caaaagttta atattgatta catctaggga cccagaagtt 1320ctgaggtgct cagaagttga aaagatttat cacttaaatg gccttaatga accgtgttcc 1380cgaaagcttt tttgcttcca tgccttccac caggcagctc cacttcaagg gtatgaatac 1440ctggttgctt gggttttaag agtatgcgac ggattgcctt tattattgaa actgttgggg 1500gcattacttt gtggaaataa tgacagattt tattgggaag acctatgtga tagtcttcaa 1560gcaaaaaaga tagaggaaaa gcttaaagtt atttacgaca cgttgggaac agaggaacaa 1620cagacctttt tggacattgc ctgtaatttg gtgggtaaaa acgcagatat ttggttaaaa 1680tcaggaaaga aaggtattat ttggtttcag attctactgg aaaagcgtct agtggaggtg 1740gatagtgaaa attgtataca gatgcatgat cttcttaaaa atttgggagg agaaattgct 1800aaggcaacaa aatcgccgcg tcctcttttt ttcggctgat tagtcgcaaa aatcccatgt 1860aagtgtaata aaaatctgtg cagagttttt taattcttca tcatttttaa gcaaaaaact 1920c 1921
<210>141
<211>278
<212>PRT
<213>辐射松(Pinus radiata)
<400>141Ala Met Glu Gly Tyr Ala Ala Asn Asn Asp Ala Glu Leu Lau Ser Lys1 5 10 15Thr Leu Gln Val Glu Gln Lys Leu Phe Tyr Phe Asp Leu Lys Glu Asn
20 25 30Pro Arg Gly Gln Tyr Leu Lys Ile Ser Glu Lys Thr Ser Gly Ser Arg
35 40 45Ser Thr Ile Ile Val Pro Ile Gly Gly Val Ala Trp Phe Leu Asp Leu
50 55 60Phe Asn Tyr Tyr Val Asp Gly Asp Asp Glu Glu Val Leu Ser Lys Glu65 70 75 80Leu Gln Leu Asp Ala Lys Val Phe Tyr Phe Asp Val Gly Val Asn Lys
85 90 95Arg Gly Arg Phe Leu Lys Ile Ser Glu Ala Ser Thr Ser Tyr Ser Arg
100 105 110Ser Thr Ile Ile Val Pro Val Gly Asn Thr Arg Lys Asp Gly Trp Ala
115 120 125Ala Phe Arg Asn Ile Leu Gly Glu Ile Asn Glu Ala Ser Gln Gln Ala
130 135 140Ser Gly Pro Ser Glu His Leu Gly Gly Leu Ser Asp Glu Val Gly Ala145 150 155 160Gly Phe Leu Ser Gly Gly Ser Gly Glu Ala Ala Phe Glu Ala Asp Thr
165 170 175Thr Gly Asp Arg Ala Met Gly Leu Thr Pro Ala Glu Asp Thr Gly Glu
180 185 190Ala Val Val Ser Lys Val Ile Arg Ala Asp Gln Lys Arg Phe Phe Phe
195 200 205Asp Leu Gly Cys Asn Asn Arg Gly Gln Phe Leu Arg Ile Ser Glu Val
210 215 220Ile Gly Pro Asp Arg Ser Ala Ile Ile Val Pro Val Ser Ala Leu Glu225 230 235 240Gln Phe His Asp Val Leu Gly His Phe Val Asp Ile Thr Arg Thr Gln
245 250 255Gly Leu Ala Ala Ala Ser Gly Ala Asn Val Arg Thr Val Ala Ala Ala
260 265 270His Arg Arg Asn Glu Asn
275
<210>142
<211>97
<212>PRT
<213>辐射松(Pinus radiata)
<400>142Ala Met Glu Gly Tyr Ala Ala Asn Asn Asp Ala Glu Leu Leu Ser Lys1 5 10 15Thr Leu Gln Val Glu Gln Lys Leu Phe Tyr Phe Asp Leu Lys Glu Asn
20 25 30Pro Arg Gly Gln Tyr Leu Lys Ile Ser Glu Lys Thr Ser Gly Ser Arg
35 40 45Ser Thr Ile Ile Val Pro Ile Gly Gly Val Ala Trp Phe Leu Asp Leu
50 55 60Phe Asn Tyr Tyr Val Asp Gly Asp Asp Glu Glu Val Leu Ser Lys Glu65 70 75 80Leu Gln Leu Asp Ala Lys Val Phe Tyr Phe Asp Val Gly Val Asn Lys
85 90 95Arg
<210>143
<211>386
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>143Ser Ser Ser Ser Ser Phe Phe Leu Leu Leu Pro Pro Ile Phe Leu Leu1 5 10 15His Ser Arg Arg Pro Ser Met Ala Ile Pro Ala Thr Ala Ala Ala Ala
20 25 30Leu Leu Leu Phe Cys Ala Val Ala Ala Val Ala Ala Ala Ala Arg Thr
35 40 45Asp Glu Glu Val Met Gly Met Tyr Glu Leu Trp Leu Ala Lys His Gly
50 55 60Lys Ala Tyr Asn Gly Leu Gly Glu Arg Glu Arg Arg Phe Glu Ile Phe65 70 75 80Arg Asp Asn Leu Arg Phe Val Asp Glu His Asn Ala Leu Asn Arg Ser
85 90 95Tyr Thr Leu Gly Met Asn Arg Phe Ala Asp Leu Thr Asn Glu Glu Tyr
100 105 110Arg Ala Val Tyr Leu Gly Thr Arg Ser Asp Pro Met Arg Arg Val Ala
115 120 125Lys Ala Ala Arg Ala Ser Gly Arg Tyr Ala Pro Arg Pro Asp Asp Met
130 135 140Leu Pro Ala Ala Val Asp Trp Arg Thr Arg Gly Ala Val Asn Lys Val145 150 155 160Lys Asp Gln Gly Ala Cys Gly Ser Cys Trp Ala Phe Ser Thr Ile Ala
165 170 175Ala Val Glu Gly Ile Asn Gln Ile Val Thr Gly Glu Phe Ile Ser Leu
180 185 190Ser Glu Gln Glu Leu Val Asp Cys Asp Arg Ala Tyr Asp Ala Gly Cys
195 200 205Asn Gly Gly Leu Met Asp Tyr Ala Phe Gln Phe Ile Ile Asp Asn Gly
210 215 220Gly Ile Asp Thr Asp Glu Asp Tyr Ser Tyr Thr Gly Val Asp Gly Thr225 230 235 240Cys Asp Ala Ser Lys Val Asn Ser Lys Val Val Ser Ile Asp Gly Tyr
245 250 255Glu Asp Val Pro Ala Phe Asp Glu Arg Ala Leu Lys Lys Ala Val Ala
260 265 270His Gln Pro Val Ser Val Ala Ile Glu Ala Gly Gly Arg Asp Phe Gln
275 280 285Leu Tyr Glu Ser Gly Val Phe Thr Gly Glu Cys Gly Thr Ala Leu Asp
290 295 300His Gly Val Ile Ala Val Gly Tyr Gly Arg Gln His Gly Ala Asp Tyr305 310 315 320Trp Leu Val Arg Asn Ser Trp Gly Ser Leu Trp Gly Glu Ser Gly Tyr
325 330 335Ile Lys Met Glu Arg Asn Leu Ala Asn Asn Tyr Phe Gly Lys Cys Gly
340 345 350Ile Ala Met Glu Ala Ser Tyr Pro Val Lys Thr Thr Gln Ash Pro Ala
355 360 365Ser Lys Tyr Ser Ser Met Gly Ser Ser Gly Gly Ile Glu Leu Val Ser
370 375 380Ser Ser385
<210>144
<211>109
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>144Leu Pro Pro Ile Phe Leu Leu His Arg Pro Cys Pro Ser Met Ala Ile1 5 10 15Pro Ala Ala Ala Ala Ala Ala Leu Leu Leu Phe Ser Ala Val Ala Ala
20 25 30Val Ala Ala Ala Ala Ala Arg Thr Asp Glu Glu Val Met Gly Met Tyr
35 40 45Glu Leu Trp Leu Val Lys His Gly Lys Ala Tyr Asn Gly Leu Gly Glu
50 55 60Arg Glu Arg Arg Phe Glu Ile Phe Arg Asp Asn Leu Arg Phe Val Asp65 70 75 80Glu His Thr Gly Leu Asn Arg Ser Tyr Ala Leu Gly Met Asn Arg Phe
85 90 95Ala Asp Leu Thr Asn Glu Glu Tyr Arg Ala Ile Tyr Leu
100 105
<210>145
<211>204
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>145Gln Ile Val Thr Gly Glu Leu Ile Ser Leu Ser Glu Gln Glu Leu Val1 5 10 15Asp Cys Asp Arg Ser Tyr Asp Ala Gly Cys Asn Gly Gly Leu Met Asp
20 25 30Tyr Ala Phe Gln Phe Ile Ile Asp Asn Gly Gly Ile Asp Thr Asp Glu
35 40 45Asp Tyr Ser Tyr Thr Gly Val Asp Gly Thr Cys Asp Ala Ser Lys Val
50 55 60Asn Ser Lys Val Val Ser Ile Asp Gly Tyr Glu Asp Val Pro Ala Phe65 70 75 80Asp Glu Arg Ala Leu Lys Lys Ala Val Ala His Gln Pro Val Ser Val
85 90 95Ala Ile Glu Ala Gly Gly Arg Asp Phe Gln Leu Tyr Glu Ser Gly Val
100 105 110Phe Thr Gly Glu Cys Gly Thr Ala Leu Asp His Gly Val Ile Ala Val
115 120 125Gly Tyr Gly Arg Gln His Gly Ala Asp Tyr Trp Leu Val Arg Asn Ser
130 135 140Trp Gly Ser Leu Trp Gly Glu Ser Gly Tyr Ile Lys Met Glu Arg Asn145 150 155 160Leu Ala Asn Asn Tyr Phe Gly Lys Cys Gly Ile Ala Met Glu Ala Ser
165 170 175Tyr Pro Val Lys Thr Ser Gln Asn Pro Ala Ser LVs TVr Ser Ser Met
180 185 190Gly Ser Ser Gly Gly Ile Glu Leu Val Ser Ser Ser
195 200
<210>146
<211>286
<212>PRT
<213>辐射松(Pinus radiata)
<400>146Ser Ser Gln Lys Lys Tyr Gly Ser Arg Gly Gly Arg Ser Thr Val Asp1 5 10 15Met Gly Ile Leu Leu Phe Phe Ala Leu Leu Ala Met Leu Ala Met Ala
20 25 30Gly Asn Ala Ser Arg Ala Asp Phe Ser Ile Ile Ser Asn Lys Asp Leu
35 40 45Arg Glu Asp Asp Ala Ile Met Glu Leu Tyr Glu Leu Trp Leu Ala Glu
50 55 60His Lys Lys Ala Tyr Asn Gly Leu Asp Glu Lys Gln Lys Arg Phe Thr65 70 75 80Val Phe Lys Asp Asn Phe Leu Tyr Ile His Glu His Asn Gln Gly Asn
85 90 95Arg Ser Tyr Lys Leu Gly Leu Asn Gln Phe Ala Asp Leu Ser His Glu
100 105 110Glu Phe Lys Ala Thr Tyr Leu Gly Ala Lys Leu Asp Thr Lys Lys Arg
115 120 125Leu Leu Arg Ser Pro Ser Pro Arg Tyr Gln Tyr Ser Asp Gly Glu Asp
130 135 140Leu Pro Lys Ser Ile Asp Trp Arg Glu Lys Gly Ala Val Ala Pro Val145 150 155 160Lys Asp Gln Gly Ala Cys Gly Ser Cys Trp Ala Phe Ser Thr Val Ala
165 170 175Ala Val Glu Gly Ile Asn Gln Ile Val Thr Gly Asp Leu Ile Ser Leu
180 185 190Ser Glu Gln Glu Leu Val Asp Cys Asp Thr Ser Tyr Asn Gln Gly Cys
195 200 205Asn Gly Gly Leu Met Asp Tyr Ala Phe Glu Phe Ile Ile Asn Asn Gly
210 215 220Gly Leu Asp Ser Glu Glu Asp Tyr Pro Tyr Thr Ala Tyr Asp Gly Ser225 230 235 240Cys Asp Ala Tyr Arg Lys Asn Ala His Val Val Thr Ile Asp Asp Tyr
245 250 255Glu Asp Val Pro Glu Asn Asp Glu Lys Ser Leu Lys Lys Ala Ala Ala
260 265 270Asn Gln Pro Ile Ser Val Ala Ile Glu Ala Ser Gly Arg Glu
275 280 285
<210>147
<211>266
<212>PRT
<213>辐射松(Pinus radiata)
<400>147Ser Ser Gln Lys Lys Tyr Gly Ser Arg Gly Gly Arg Ser Thr Val Asp1 5 10 15Met Gly Ile Leu Leu Phe Phe Ala Leu Leu Ala Met Ser Ala Met Ala
20 25 30Gly Ser Ala Ser Arg Ala Asp Phe Ser Ile Ile Ser Asn Lys Asp Leu
35 40 45Arg Glu Asp Asp Ala Ile Met Glu Leu Tyr Glu Leu Trp Leu Ala Glu
50 55 60His Lys Lys Ala Tyr Asn Gly Leu Asp Glu Lys Gln Lys Arg Phe Thr65 70 75 80Val Phe Lys Asp Asn Phe Leu Tyr Ile His Glu His Asn Gln Gly Asn
85 90 95Arg Ser Tyr Lys Leu Gly Leu Asn Gln Phe Ala Asp Leu Ser His Glu
100 105 110Glu Phe Lys Ala Thr Tyr Leu Gly Ala Lys Leu Asp Thr Lys Lys Arg
115 120 125Leu Leu Arg Ser Pro Ser Pro Arg Tyr Gln Tyr Ser Asp Gly Glu Asp
130 135 140Leu Pro Lys Ser Ile Asp Trp Arg Glu Lys Gly Ala Val Ala Pro Val145 150 155 160Lys Asp Gln Gly Ala Cys Gly Ser Cys Trp Ala Phe Ser Thr Val Ala
165 170 175Ala Val Glu Gly Ile Asn Gln Ile Val Thr Gly Asp Leu Ile Ser Leu
180 185 190Ser Glu Gln Glu Leu Val Asp Cys Asp Thr Ser Tyr Asn Gln Gly Cys
195 200 205Asn Gly Gly Leu Met Asp Tyr Ala Phe Glu Phe Ile Ile Asn Asn Gly
210 215 220Gly Leu Asp Ser Glu Glu Asp Tyr Pro Tyr Thr Ala Tyr Asp Gly Ser225 230 235 240Cys Asp Ala Tyr Arg Lys Asn Ala His Val Val Thr Ile Asp Asp Tyr
245 250 255Glu Asp Val Pro Glu Asn Asp Glu Lys Ser
260 265
<210>148
<211>302
<212>PRT
<213>辐射松(Pinus radiata)
<400>148Asp Cys Ile Ser Met Gly Ile Leu Leu Leu Phe Ala Leu Leu Ala Leu1 5 10 15Phe Ala Met Ala Gly Ser Ala Ser Arg Ala Asp Phe Ser Ile Ile Gly
20 25 30Tyr Asp Ser Lys Asp Leu Arg Glu Asp Asp Ala Ile Met Glu Leu Tyr
35 40 45Glu Leu Trp Leu Ala Gln His Arg Lys Ala Tyr Asn Gly Leu Asp Glu
50 55 60Lys Gln Lys Arg Phe Ser Val Phe Lys Asp Asn Phe Leu Tyr Ile His65 70 75 80Gln His Asn Asn Gln Gly Asn Pro Ser Phe Lys Met Gly Leu Asn Gln
85 90 95Phe Ala Asp Leu Ser His Glu Glu Phe Lys Ala Thr Tyr Leu Gly Cys
100 105 110Glu Leu Asp Thr Lys Lys Arg Leu Ser Lys Ser Pro Ser Pro Arg Tyr
115 120 125Gln Tyr Ser Glu Gly Glu Asn Leu Pro Glu Ser Val Asp Trp Arg Glu
130 135 140Lys Gly Ala Val Ala Ala Val Lys Asp Gln Gly Ser Cys Gly Ser Cys145 150 155 160Trp Ala Phe Ser Thr Val Ala Ala Val Glu Gly Ile Asn Gln Ile Val
165 170 175Thr Gly Asn Leu Thr Ser Leu Ser Glu Gln Glu Leu Val Asp Cys Asp
180 185 190Thr Ser Tyr Asn Gln Gly Cys Asu Gly Gly Leu Met Asp Tyr Ala Phe
195 200 205Gln Phe Ile Ile Asp Asn Gly Gly Leu Asp Ser Glu Asp Asp Tyr Pro
210 215 220Tyr Met Ala Asn Asp Gly Ser Cys Asp Ala Tyr Arg Lys Asn Ala His225 230 235 240Val Val Thr Ile Asp Ser Tyr Glu Asp Val Pro Glu Asn Asp Glu Lys
245 250 255Ser Leu Lys Lys Ala Ala Ala His Gln Pro Ile Ser Val Ala Ile Glu
260 265 270Ala Ser Gly Arg Ala Phe Gln Phe Tyr Glu Ser Gly Val Phe Thr Ser
275 280 285Thr Cys Gly Thr Gln Leu Asp His Gly Val Thr Leu Val Gly
290 295 300
<210>149
<211>500
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>149Phe Ser Leu Ser Ile Leu Leu Ser Leu Ser Ser Asp Ala Pro Ile Gly1 5 10 15Tyr Ala Glu Ala Lys Lys Met Asp Leu Lys Ser Pro Pro Ala Ala Ala
20 25 30Ala Val Ala Val Leu Ala Leu Ala Leu Ala Leu Thr Thr Ile Ala Ser
35 40 45Ala Leu Asp Met Ser Ile Val Ser Tyr Asp Arg Ala His Gly Asp Arg
50 55 60Ser Ser Ser Ser Ser Ser Ser Trp Arg Ser Asp Asp Glu Val Met Ala65 70 75 80Val Tyr Glu Ser Trp Leu Ala Lys His Gly Lys Ala Tyr Asn Ala Leu
85 90 95Gly Glu Lys Glu Lys Arg Phe Gln Val Phe Lys Asp Asn Leu Arg Phe
100 105 110Ile Asp Asp His Asn Ala Gly Gly Asp Arg Thr Tyr Thr Val Gly Leu
115 120 125Asn Gln Phe Ala Asp Leu Thr Asn Glu Glu Tyr Arg Ser Met Tyr Leu
130 135 140Gly Ala Arg Met Asp Arg Ser Gly Arg Arg Leu Gly Arg Ala Arg Ser145 150 155 160Asp Arg Tyr Ala Val Ala Ala Gly Glu Glu Leu Pro Ala Ser Val Asp
165 170 175Trp Arg Lys Glu Gly Ala Val Val Asp Val Lys Asp Gln Gly Ser Cys
180 185 190Gly Ser Cys Trp Ala Phe Ser Thr Ile Ala Ala Val Glu Gly Ile Asn
195 200 205Lys Leu Val Thr Gly Asp Leu Ile Ser Leu Ser Glu Gln Glu Leu Val
210 215 220Asp Cys Asp Thr Ser Tyr Asn Glu Gly Cys Asn Gly Gly Leu Met Asp225 230 235 240Tyr Ala Phe Glu Phe Ile Ile Asn Asn Gly Gly Ile Asp Thr Glu Glu
245 250 255Asp Tyr Pro Tyr Arg Ala Val Asp Ser Thr Cys Asp Gln Tyr Arg Lys
260 265 270Asn Ala Lys Val Val Thr Ile Asp Asp Tyr Glu Asp Val Pro Glu Asn
275 280 285Asp Glu Lys Ala Leu Gln Lys Ala Val Ala Asn Gln Pro Val Ser Val
290 295 300Ala Ile Glu Ala Gly Gly Arg Glu Phe Gln Phe Tyr Asp Ser Gly Ile305 310 315 320Phe Thr Gly Lys Cys Gly Thr Ala Leu Asp His Gly Val Thr Ala Val
325 330 335Gly Tyr Gly Thr Glu Asn Gly Val Asp Tyr Trp Ile Val Lys Asn Ser
340 345 350Trp Gly Gly Ser Trp Gly Glu Gln Gly Tyr Ile Lys Met Ala Arg Asn
355 360 365Val Ala Asn Ser Pro Thr Gly Lys Cys Gly Ile Ala Met Glu Ala Ser
370 375 380Tyr Pro Ile Lys Lys Gly Gln Asn Pro Pro Asn Pro Gly Pro Ser Pro385 390 395 400Pro Ser Pro Val Lys Pro Pro Thr Val Cys Asp Asn Tyr Tyr Ser Cys
405 410 415Pro Glu Ser Asn Thr Cys Cys Cys Val Tyr Glu Tyr Ala Asn Tyr Cys
420 425 430Phe Ala Trp Gly Cys Cys Pro Leu Glu Ala Ala Thr Cys Cys Glu Asp
435 440 445His Tyr Ser Cys Cys Pro Gln Asp Phe Pro Val Cys Asn Val Asn Ala
450 455 460Gly Thr Cys Gln Met Ser Lys Asp Asn Pro Leu Gly Val Lys Ala Leu465 470 475 480Lys Arg Thr Pro Ala Lys Phe His Trp Ala Phe Gly Ser Asp Gly Gln
485 490 495Lys Ser Ser Ala
500
<210>150
<211>200
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>150Asp Gln Gly Asp Cys Gly Ser Cys Trp Ala Phe Ser Ala Ala Ala Ala1 5 10 15Met Glu Gly Ile Thr Met Ile Lys Lys Gly Lys Leu Val Pro Leu Ser
20 25 30Val Gln Glu Leu Val Asp Cys Asp Val Asp Asp Asn Gly Cys His Gly
35 40 45Gly Leu Met Asp Arg Ala Phe Lys Phe Ile Lys Ser Lys Gly Gly Leu
50 55 60Ser Thr Glu Ala Asn Tyr Pro Tyr Gln Ala Asn Asn Gly Thr Cys Asn65 70 75 80Thr Ala Lys Met Ala Asn Pro Val Ala Ser Ile Thr Gly Tyr Gln Asp
85 90 95Val Pro Ala Asn Asn Glu Lys Ala Leu Leu Gln Ala Val Ala Asn Gln
100 105 110Pro Val Ser Val Ala Ile Glu Gly Ser Gly Phe Asn Phe Gln Phe Tyr
115 120 125Ser Ser Gly Val Phe Ser Gly Ser Cys Gly Thr Ser Ile Asp His Ala
130 135 140Val Thr Ala Val Gly Tyr Gly Lys Thr Ser Arg Gly Thr Lys Tyr Trp145 150 155 160Leu Leu Lys Asn Ser Trp Gly Thr Gly Trp Gly Glu Ser Gly Tyr Met
165 170 175Arg Ile Gln Arg Asp Val Ser Ser Asn Ala Gly Leu Cys Gly Leu Ala
180 185 190Met Glu Ala Ser Tyr Pro Thr Ala
195 200
<210>151
<211>198
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>151Asn Tyr Leu Arg Thr Asn Lys Leu Leu Ser Leu Ser Glu Gln Glu Leu1 5 10 15Val Asp Cys Asp Asn Thr Gln Asn His Gly Cys Asn Gly Gly Leu Met
20 25 30Asp Ile Ala Phe Glu Phe Ile Lys Gln Lys Gly Gly Ile Thr Ser Glu
35 40 45Ser Asn Tyr Pro Tyr Gln Ala Ser Asn Gly Thr Cys Asp Ala Ala Lys
50 55 60Glu Asn Ser Pro Val Val Ser Ile Asp Gly His Glu Asn Val Pro Ala65 70 75 80Asn Asp Glu Asp Ala Leu Gln Lys Ala Val Ala Asn Gln Pro Val Ser
85 90 95Val Ala Ile Glu Ala Ser Gly Ala Asp Phe Gln Phe Tyr Ser Glu Gly
100 105 110Val Phe Thr Gly Ser Cys Gly Thr His Leu Asp His Gly Val Ala Ile
115 120 125Val Gly Tyr Gly Ser Thr Leu Gln Gly Thr Lys Tyr Trp Ile Val Arg
130 135 140Asn Ser Trp Gly Pro Glu Trp Gly Glu Lys Gly Tyr Leu Arg Met Glu145 150 155 160Arg Gly Ile Glu Ala Lys Glu Gly Leu Cys Gly Ile Ala Met Glu Ala
165 170 175Ser Tyr Pro Ile Lys Asn Ser Ser Asp Asn Pro Ala Gly Val Ser Ser
180 185 190Pro Val Lys Asp Glu Leu
195
<210>152
<211>381
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>152Lys Thr Gln Gly Arg Arg Lys Glu Ser Val Gln Asn His Leu Leu Lys1 5 10 15Glu Ser Tyr Asn Ser Pro Ser His Ser Asn Cys Val Leu Asp Ser Thr
20 25 30Gln Ser Thr Thr Met Ala Lys Gln Asn Gln Phe Ser Phe Leu Thr Ser
35 40 45Ala Ala Leu Leu Val Ile Ile Val Ser Val Ser Glu Thr Leu Cys Arg
50 55 60Pro Leu Glu Glu Glu Gln Leu Leu Lys Gln His Glu Glu Trp Met Ala65 70 75 80Ile His Gly Arg Val Tyr Lys Asp Ala Val Glu Lys Ala Lys Arg Tyr
85 90 95Glu Ile Phe Lys Glu Asn Val Lys Arg Ile Asn Ala Phe Asn Asn Gly
100 105 110Lys Asp Val Gly Tyr Lys Met Ala Val Asn Lys Phe Ala Asp Leu Thr
115 120 125Asn Glu Glu Phe Arg Ala Ser Tyr Thr Gly Tyr Lys Arg Arg Pro Thr
130 135 140Arg Val Leu Ser Ser Gly Glu Lys Lys Pro Phe Lys Tyr Ala Asn Phe145 150 155 160Thr Ala Ile Pro Ala Ala Leu Asp Trp Arg Thr Lys Lys Ala Val Thr
165 170 175Pro Val Lys Asp Gln Gly Gly Cys Gly Ser Cys Trp Ala Phe Ser Ala
180 185 190Val Ala Ala Met Glu Gly Ile Thr Met Ile Lys Lys Gly Lys Leu Val
195 200 205Pro Leu Ser Val Gln Glu Leu Val Asp Cys Asp Asp Asn Asp Glu Gly
210 215 220Cys Arg Gly Gly Leu Met Asp Ser Ala Phe Lys Phe Ile Val Ser Asn225 230 235 240Gly Gly Leu Thr Thr Glu Ala Asn Tyr Pro Tyr Gln Gly Asn Asp Gly
245 250 255Thr Cys Asn Thr Ala Lys Thr Ala Asn Pro Ala Ala Ser Ile Thr Gly
260 265 270Tyr Gln Asp Val Pro Ala Asn Asn Glu Lys Ala Leu Leu Gln Ala Val
275 280 285Ala Asn Gln Pro Val Ser Val Ala Ile Glu Gly Gly Gly Tyr Asn Phe
290 295 300Gln Phe Tyr Ser Ser Gly Val Phe Thr Gly Ser Cys Gly Thr Asp Ile305 310 315 320Asp His Ala Val Thr Ala Val Gly Tyr Gly Lys Thr Ser Gly Ser Gly
325 330 335Gly Thr Lys Tyr Trp Leu Met Lys Asn Ser Trp Gly Thr Gly Trp Gly
340 345 350Glu Lys Gly Tyr Met Arg Ile Gln Lys Asp Val Ser Ser Lys Ala Gly
355 360 365Leu Cys Gly Leu Ala Thr Glu Ala Ser Tyr Pro Ala Ala
370 375 380
<210>153
<211>396
<212>PRT
<213>辐射松(Pinus radiata)
<400>153Lys Lys Ile Val Phe Pro Phe Leu His Thr Asn Ser His Tyr Leu Ser1 5 10 15Glu Phe Cys Cys Tyr Phe Val Met Ala Ser Val Ser Lys Ala Thr Leu
20 25 30Leu Leu Phe Leu Ala Thr Thr Leu Trp Thr Leu Ser Ala Asn Ala Ser
35 40 45Asp Ser Ser Pro Gly Phe Thr Asp Glu Asp Leu Lys Ser Glu Glu Ser
50 55 60Leu Arg Leu Leu Tyr Asp Lys Trp Ala Leu Arg His Arg Thr Thr Arg65 70 75 80Ser Leu Asp Ser Asp Glu His Ala Lys Arg Phe Glu Ile Phe Lys Asp
85 90 95Asn Val Lys Tyr Ile Asp Ser Val Asn Gln Lys Asp Gly Pro Tyr Lys
100 105 110Leu Gly Leu Asn Lys Phe Thr Asp Leu Ser Asn Glu Glu Phe Lys Ala
115 120 125Met His Met Thr Thr Arg Met Glu Lys His Lys Ser Leu Arg Arg Asp
130 135 140Arg Gly Thr His Ser Gly Ser Phe Met Tyr Gln Asn Ser Asp Asn Leu145 150 155 160Pro Glu Ser Ile Asp Trp Arg Glu Lys Gly Ala Val Asn Pro Val Lys
165 170 175Asn Gln Gly Gln Cys Gly Ser Cys Trp Ala Phe Ser Thr Ile Ala Ser
180 185 190Val Glu Gly Ile Ser Tyr Val Lys Thr Gly Lys Leu Val Ser Leu Ser
195 200 205Glu Gln Gln Leu Val Asp Cys Ser Lys Glu Asn Ala Gly Cys Asn Gly
210 215 220Gly Leu Met Asp Ser Ala Phe Gln Tyr Ile Ile Asp Asn Gly Gly Ile225 230 235 240Val Ala Glu Asp Glu Tyr Pro Tyr Thr Ala Glu Ala Ser Glu Cys Ser
245 250 255Pro Ser Lys Val Lys Pro Asn Ala Ile Ala Ala Thr Ile Asp Gly Phe
260 265 270Glu Asp Val Pro Ala Asn Asn Glu Lys Ala Leu Lys Glu Ala Val Gly
275 280 285His Gln Pro Val Ser Val Ala Ile Glu Ala Ser Gly Lys Asp Phe Gln
290 295 300Phe Tyr Ser Lys Gly Val Phe Thr Gly Glu Cys Gly Thr Glu Leu Asp305 310 315 320His Gly Val Val Ala Val Gly Tyr Gly Lys Ser Pro Glu Gly Ile Asn
325 330 335Tyr Trp Ile Val Arg Asn Ser Trp Gly Pro Glu Trp Gly Glu Glu Gly
340 345 350Tyr Ile Lys Met Gln Arg Asp Ile Glu Ala Val Glu Gly Lys Cys Gly
355 360 365Ile Ala Met Gln Ala Ser Tyr Pro Thr Lys Lys Thr Gln Gly Ile Asp
370 375 380Ile Glu Leu Asp Val Ala His Val Ser Asp Glu Leu385 390 395
<210>154
<211>185
<212>PRT
<213>巨杉(Eucalyptus grandis)Glu Asp Tyr Pro Tyr Lys Ala Val Asp Gly Lys Cys Asp Gln Tyr Arg1 5 10 15Lys Asn Ala Lys Val Val Thr Ile Asp Asp Tyr Glu Asp Val Pro Ala
20 25 30Asn Asp Glu Lys Ala Leu Gln Lys Ala Val Ala Asn Gln Pro Val Ser
35 40 45Val Ala Ile Glu Ala Gly Gly Arg Ala Phe Gln Leu Tyr Gln Ser Gly
50 55 60Val Phe Ser Gly Arg Cys Gly Thr Ala Leu Asp His Gly Val Thr Ala65 70 75 80Val Gly Tyr Gly Thr Glu Lys Gly Met Asn Tyr Trp Ile Val Lys Asn
85 90 95Ser Trp Gly Lys Ser Trp Gly Glu Gln Gly Tyr Ile Arg Met Glu Arg
100 105 110Ser Leu Thr Asn Thr Ile Thr Gly Lys Cys Gly Ile Ala Met Glu Ala
115 120 125Ser Tyr Pro Ile Lys Asn Gly Pro Asn Pro Pro Asn Pro Gly Pro Ser
130 135 140Pro Pro Ser Pro Ile Lys Pro Pro Thr Thr Cys Asp Arg Tyr Tyr Ser145 150 155 160Cys Ala Glu Ser Thr Thr Cys Cys Cys Val Tyr Gln Tyr Ala Asn Tyr
165 170 175Cys Phe Ala Trp Gly Cys Cys Pro Leu
180 185
<210>155
<211>160
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>155Ser Thr Pro Arg Asn Ser Ser Gln Val Asn Ser Phe Lys Tyr Gln Gly1 5 10 15Ser Asn Ser Ile Pro Glu Ser Ile Asp Trp Val Gln Lys Gly Ala Val
20 25 30Asn Pro Ile Lys Tyr Gln Arg Gln Cys Gly Ser Cys Trp Ser Phe Ser
35 40 45Val Val Ala Ala Val Glu Ala Ile Thr Gln Ile Thr Thr Gly Val Leu
50 55 60Pro Ser Leu Ser Glu Gln Gln Leu Ile Asp Cys Thr Thr Asp Gly Asn65 70 75 80His Gly Cys Glu Gly Gly Ser Met Asp Asn Gly Phe Glu Tyr Ile Ile
85 90 95Asn Asn Asn Gly Ile Ser Ser Glu Thr Asn Tyr Pro Tyr Val Gly Val
100 105 110Asp Gly Thr Cys Asn Val Gln Ala Ser Ser Val Ala Glu Ala Lys Ile
115 120 125Ser Asp His Lys Asp Val Pro Ser Asn Glu Asp Asp Met Leu Lys Ala
130 135 140Val Ala Met Gln Pro Val Ser Ala Ala Ile Asp Ala Asn Gly Asp Val145 150 155 160
<210>156
<211>272
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>156Ser His Ser Asn Cys Val Leu Asp Ser Thr His Thr Ser Thr Met Ala1 5 10 15Lys Gln Asn Gln Leu Pro Phe Leu Thr Leu Ala Thr Leu Leu Val Ile
20 25 30Ile Val Phe Val Ser Glu Thr Leu Cys Arg Pro Leu Gly Glu Glu His
35 40 45Leu Leu Lys Gln His Glu Gln Trp Met Ala Val His Gly Arg Val Tyr
50 55 60Lys Asp Ala Asp Glu Lys Ala Lys Arg Tyr Glu Ile Phe Lys Gln Asn65 70 75 80Val Asn Arg Ile Asn Ala Phe Asn Asn Asp Lys Asp Ala Ala Tyr Lys
85 90 95Leu Ala Val Asn Lys Phe Ala Asp Leu Thr Asn Glu Glu Phe Arg Ala
100 105 110Ser Phe Thr Gly Tyr Lys Arg Arg Ser Thr Arg Val Leu Thr Ser Val
115 120 125Asp Glu Lys Pro Phe Lys Tyr Ala Asn Phe Thr Ala Ala Pro Pro Val
130 135 140Leu Asp Trp Arg Thr Lys Lys Ala Val Thr Ser Val Lys Asp Gln Ser145 150 155 160Ser Cys Gly Ala Cys Trp Ala Phe Ser Ala Val Ala Ala Met Glu Gly
165 170 175Ile Thr Met Leu Lys Lys Gly Lys Leu Val Ser Leu Ser Glu Gln Glu
180 185 190Leu Val Asp Cys Asp Val Asn Gly Val Asn Gln Gly Cys Glu Gly Gly
195 200 205Leu Met Asp Ser Ala Phe Gln Phe Ile Lys Ser Lys Gly Gly Leu Thr
210 215 220Ser Glu Ala Asn Tyr Pro Phe Gln Gly Asn Asp Gly Thr Cys Arg Thr225 230 235 240Ala Lys Ala Ala Asn Ile Val Ala Ser Ile Ala Gly Tyr Gln Asp Val
245 250 255Pro Ala Asn Asn Glu Lys Ala Leu Leu Gln Ala Arg Gly Glu Pro Ala
260 265 270
<210>157
<211>122
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>157Ser His Ser Asn Cys Val Leu Asp Ser Thr His Thr Ser Thr Met Ala1 5 10 15Lys Gln Asn Gln Leu Pro Phe Leu Thr Leu Ala Thr Leu Leu Val Ile
20 25 30Ile Val Ser Val Ser Glu Thr Leu Cys Arg Pro Leu Gly Glu Glu His
35 40 45Leu Leu Lys Gln His Glu Gln Trp Met Ala Val His Gly Arg Val Tyr
50 55 60Lys Asp Ala Asp Glu Lys Ala Lys Arg Tyr Glu Ile Phe Lys Gln Asn65 70 75 80Val Asn Arg Ile Asn Ala Phe Asn Asn Asp Lys Asp Ala Gly Tyr Lys
85 90 95Leu Ala Val Asn Lys Phe Ala Asp Leu Thr Asn Glu Glu Phe Arg Ala
100 105 110Ser Phe Thr Gly Tyr Lys Arg Arg Ser Thr
115 120
<210>158
<211>378
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>158Pro Thr His Ile Ser Trp Asn Pro Gln Arg Glu Arg Glu Arg Glu Arg1 5 10 15Glu Arg Glu Met Ala Arg Ala Arg Leu Leu Cys Ser Ala Val Leu Leu
20 25 30Leu Val Ala Val Val Val Ser Ala Ala Ala Ser Ser Phe Glu Glu Ser
35 40 45Asn Pro Ile Arg Leu Phe Pro Asp Gly Gly Leu Arg Asp Leu Glu Ser
50 55 60Ser Ile Val Gln Ile Val Gly Arg Thr Arg His Ala Phe Ser Phe Ala65 70 75 80Arg Phe Ala Asn Arg Tyr Gly Lys Arg Tyr Glu Thr Ala Glu Glu Ile
85 90 95Lys Leu Arg Phe Glu Ile Phe Arg Glu Asn Leu Lys Leu Ile Arg Ser
100 105 110Thr Asn Lys Lys Gly Leu Pro Tyr Thr Leu Gly Val Asn Lys Phe Ala
115 120 125Asp Trp Ser Trp Glu Glu Phe Arg Arg His Arg Leu Gly Ala Ala Gln
130 135 140Asn Cys Ser Ala Thr Thr Lys Gly Asn His Lys Leu Thr Asp Glu Ala145 150 155 160Leu Pro Glu Met Lys Asp Trp Arg Glu Lys Gly Ile Val Ser Pro Ile
165 170 175Lys Asp Gln Gly His Cys Gly Ser Cys Trp Thr Phe Ser Thr Thr Gly
180 185 190Ala Leu Glu Ala Ala Tyr His Gln Ala Phe Gly Lys Gln Ile Ser Leu
195 200 205Ser Glu Gln Gln Leu Val Asp Cys Ala Gly Ala Phe Asn Asn Phe Gly
210 215 220Cys Ser Gly Gly Leu Pro Ser Gln Ala Phe Glu Tyr Val Lys Tyr Asn225 230 235 240Gly Gly Leu Asp Thr Glu Glu Ala Tyr Pro Tyr Thr Ala Val Asp Gly
245 250 255Ser Cys Lys Phe Ser Ala Asp Asn Val Gly Val Gln Val Leu Asp Ser
260 265 270Val Asn Ile Thr Leu Gly Ala Glu Asp Glu Leu Lys His Ala Val Ala
275 280 285Phe Val Arg Pro Val Ser Val Ala Phe Gln Val Val Lys Asp Phe Arg
290 295 300Leu Tyr Lys Ser Gly Val Tyr Thr Ser Asp Thr Cys Gly Ser Thr Ser305 310 315 320Met Asp Val Asn His Ala Val Leu Ala Val Gly Tyr Gly Val Glu Asp
325 330 335Gly Val Pro Phe Trp Leu Ile Lys Asn Ser Trp Gly Ala Asp Trp Gly
340 345 350Asp His Gly Tyr Phe Lys Met Glu Met Gly Lys Asn Met Cys Gly Val
355 360 365Ala Thr Cys Ala Ser Tyr Pro Val Val Ala
370 375
<210>159
<211>129
<212>PRT
<213>辐射松(Pinus radiata)
<400>159Ala Met His Met Lys Thr Arg Met Glu Lys His Arg Ser Leu Arg Gly1 5 10 15Asp Arg Gly Val Gln Gly Gly Ser Phe Met Tyr Gln Asn Ser Lys His
20 25 30Leu Pro Ala Ser Ile Asp Trp Arg Lys Lys Gly Ala Val Thr Pro Val
35 40 45Lys Asn Gln Gly Gln Cys Gly Ser Cys Trp Ala Phe Ser Thr Val Ala
50 55 60Ser Val Glu Gly Ile Asn Tyr Ile Lys Thr Gly Lys Leu Val Ser Leu65 70 75 80Ser Glu Gln Gln Leu Val Asp Cys Ser Lys Glu Asn Ala Gly Cys Asn
85 90 95Gly Gly Leu Met Asp Asn Ala Phe Gln Tyr Ile Ile Asp Asn Gly Gly
100 105 110Ile Val Ser Glu Ala Glu Tyr Pro Tyr Thr Ala Glu Ala Arg Glu Cys
115 120 125Ser
<210>160
<211>348
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>160Glu Asn Ile Ile Met Gly Ser Ser Pro Arg Thr His His Gln Leu Gly1 5 10 15Leu Ala Ile Leu Phe Phe Ala Ser Leu Ala Arg Leu Ser Leu Ser His
20 25 30Ala Ser Leu Pro Ser Glu Tyr Ser Ile Ile Gly His Gly Gln Asp Pro
35 40 45Asn Gly Ala Val Ser Asp Glu Arg Ala Val Glu Leu Phe Arg Arg Trp
50 55 60Gln Ala Gln His Lys Lys Val Tyr Lys His Ala Gly Glu Ala Glu Arg65 70 75 80Arg Leu Glu Asn Phe Lys Arg Asn Leu Arg Tyr Val Met Glu Arg Ser
85 90 95Arg Arg Asp Gly Gly Lys Gln His Gly Val Gly Leu Asn Lys Phe Ala
100 105 110Asp Leu Ser Asn Glu Glu Phe Arg Gln Arg Tyr Leu Ser Lys Val Lys
115 120 125Lys Ser Val Asn Gln Lys Trp Arg Ala Lys Arg Glu Ser Leu Met Arg
130 135 140Asn Lys Arg Lys Gly Ala Glu Ser Cys Lys Ala Pro Ser Ser Leu Asp145 150 155 160Trp Arg Asn Tyr Gly Ile Val Thr Gly Val Lys Asp Gln Gly Glu Cys
165 170 175Gly Ser Cys Trp Ala Phe Ser Ser Thr Gly Ala Met Glu Gly Ile Asn
180 185 190Ala Leu Lys Ser Gly Asp Leu Ile Ser Leu Ser Glu Gln Glu Leu Val
195 200 205Asp Cys Asp Thr Thr Asn Asp Gly Cys Asp Gly Gly Tyr Met Asp Tyr
210 215 220Ala Phe Glu Trp Val Ile Asn Asn Gly Gly Ile Asp Ser Glu Glu Asp225 230 235 240Tyr Pro Tyr Thr Ser Val Phe Gly Glu Gly Gly Ile Cys Asn Val Thr
245 250 255Lys Glu Glu Asn Ash Lys Ala Val Thr Ile Asp Gly Tyr Val Asp Val
260 265 270Tyr Pro Ser Asp Asp Gly Leu Leu Cys Thr Val Ile Gln Gln Pro Ile
275 280 285Ser Val Gly Met Asp Gly Ser Ala Ile Asp Phe Gln Leu Tyr Thr Gly
290 295 300Gly Ile Tyr Asp Gly Ser Cys Ser Ala Asn Pro Asp Asp Ile Asp His305 310 315 320Ala Val Leu Ile Val Gly Tyr Gly Ser Glu Gly Gly Glu Asp Tyr Trp
325 330 335Ile Val Lys Glu Leu Leu Gly Glu Gln Ile Trp Gly
340 345
<210>161
<211>248
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>161Glu Asn Ile Ile Met Gly Ser Ser Pro Arg Thr His His Gln Leu Gly1 5 10 15Leu Ala Ile Leu Val Phe Ala Ser Leu Ala Arg Leu Ser Leu Ser His
20 25 30Ala Ser Leu Pro Ser Glu Tyr Ser Ile Ile Gly His Gly Gln Asp Pro
35 40 45Asn Gly Ala Val Ser Asp Glu Arg Ala Val Glu Leu Phe Arg Arg Trp
50 55 60Gln Ala Gln His Lys Lys Val Tyr Lys His Ala Gly Glu Ala Glu Arg65 70 75 80Arg Leu Glu Asn Phe Lys Arg Asn Leu Arg Tyr Val Met Glu Arg Ser
85 90 95Arg Arg Asp Gly Gly Lys Gln His Gly Val Gly Leu Asn Lys Phe Ala
100 105 110Asp Leu Ser Asn Glu Glu Phe Arg Gln Leu Tyr Leu Ser Lys Val Lys
115 120 125Lys Ser Val Asn Gln Lys Trp Arg Ala Lys Arg Glu Ser Leu Met Arg
130 135 140Asn Lys Arg Lys Gly Ala Glu Ser Cys Lys Ala Pro Ser Ser Leu Asp145 150 155 160Trp Arg Asn Tyr Gly Ile Val Thr Gly Val Lys Asp Gln Gly Glu Cys
165 170 175Gly Ser Cys Trp Ala Phe Ser Ser Thr Gly Ala Met Glu Gly Ile Asn
180 185 190Ala Leu Lys Ser Gly Asp Leu Ile Ser Leu Ser Glu Gln Glu Leu Val
195 200 205Asp Cys Asp Thr Thr Asn Asp Gly Cys Asp Gly Gly Tyr Met Asp Tyr
210 215 220Ala Phe Glu Trp Val Ile Asn Asn Gly Gly Ile Asp Ser Glu Glu Asp225 230 235 240Tyr Pro Tyr Thr Ser Val Phe Gly
245
<210>162
<211>225
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>162Arg Glu Arg Glu Arg Met Ala Gly Ala Arg Phe Leu Cys Ser Phe Leu1 5 10 15Leu Leu Leu Thr Ala Cys Ser Ala Thr Ala Ala Gly Phe Gln Gly Ala
20 25 30Asp Leu Glu Ser Ser Ile Leu Gln Thr Val Gly His Gly Arg Pro Ala
35 40 45Leu Ser Phe Val Asp Phe Ala Ser Arg Tyr Glu Lys Arg Tyr Glu Thr
50 55 60Ala Glu Glu Ile Lys Leu Arg Phe Asp Asn Tyr Arg Glu Asn Leu Lys65 70 75 80Leu Ile Arg Ser Thr Asn Gln Lys Gly Leu Pro Tyr Thr Leu Ala Val
85 90 95Asn Gln Tyr Ala Asp Trp Ser Trp Glu Glu Phe Lys Thr His Arg Leu
100 105 110Gly Ala Ser Gln Asp Cys Ser Ala Thr Thr Lys Gly Ser His Lys Leu
115 120 125Thr Asp Ala Val Leu Pro Lys Thr Lys Asp Trp Arg Lys Glu Gly Ile
130 135 140Val Ser Pro Val Lys Asn Gln Gly Gly Cys Gly Ser Cys Trp Ser Phe145 150 155 160Ser Ala Thr Gly Ala Leu Glu Ala Ala Tyr His Gln Ala His Gly Lys
165 170 175Gly Ile Ser Leu Ser Glu Gln Gln Leu Val Asp Cys Ala Thr Ala Phe
180 185 190Asn Asn Phe Gly Cys Asp Gly Gly Leu Pro Ser Gln Ala Phe Glu Tyr
195 200 205Ile Lys Tyr Asn Gly Gly Leu Glu Thr Glu Glu Ala Tyr Pro Tyr Thr
210 215 220Ala225
<210>163
<211>174
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>163His Ser Leu Trp Lys Pro Gln Gly Glu Arg Glu Arg Glu Arg Met Ala1 5 10 15Gly Ala Arg Phe Leu Cys Ser Phe Leu Leu Val Val Thr Ala Cys Ser
20 25 30Ala Ala Ala Ala Gly Phe Glu Gly Ala Asp Leu Glu Ser Ser Ile Leu
35 40 45Gln Thr Val Gly His Thr Arg Pro Ala Leu Ser Phe Val Asp Phe Ala
50 55 60Arg Gly His Gly Lys Thr Tyr Lys Thr Ala Glu Glu Ile Lys Leu Arg65 70 75 80Phe Asp Asn Tyr Arg Glu Asn Leu Lys Leu Ile Arg Ser Thr Asn Gln
85 90 95Lys Gly Leu Pro Tyr Thr Leu Ala Val Asn Gln Tyr Ala Asp Trp Ser
100 105 110Trp Glu Glu Phe Lys Thr His Arg Leu Gly Ala Ser Gln Asp Cys Ser
115 120 125Ala Thr Thr Lys Gly Ser His Lys Leu Thr Asp Asp Val Leu Pro Glu
130 135 140Thr Lys Asp Trp Glu Arg Lys Gly His Cys Arg Pro Gln Leu Lys Ile145 150 155 160Lys Ala Ala Cys Gly Ser Cys Trp Ser Phe Ser Ala Thr Gly
165 170
<210>164
<211>130
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>164Leu Asp Trp Val Ala Lys Gly Ala Val Asn Ala Ile Lys Asp Gln Gly1 5 10 15Arg Cys Gly Ser Cys Trp Ala Phe Ser Ala Val Ala Ala Ile Glu Ser
20 25 30Ile Thr Gln Ile Lys Thr Gly Lys Leu Leu Glu Leu Ser Glu Gln Gln
35 40 45Leu Val Asp Cys Thr Ile Glu Asn Tyr Gly Cys Ser Gly Gly Trp Met
50 55 60Asp Thr Ala Phe Asp Tyr Ile Ile Gln Asn Gly Gly Ile Ser Ser Glu65 70 75 80Thr Asn Tyr Pro Tyr Asn Ser Ser Asp Gly Thr Cys Asn Ala His Met
85 90 95Ala Ser Leu Ser Val Ala Lys Ile Val Gly Tyr Glu Asp Val Pro Asp
100 105 110Asn Asn Glu Gly Glu Ile Leu Lys Ala Val Ala Met Gln Pro Val Ser
115 120 125Val Ala
130
<210>165
<211>278
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>165Ser Ser Ser Ala His Ser Ser Pro Thr Met Asn Arg Phe Leu Ser Leu1 5 10 15Leu Ala Leu Phe Ser Leu Ala Ile Val Ser Ala Tyr Ala Ser Ser Glu
20 25 30Val Asp Gly Asp Ala Leu Ile Arg Gln Val Val Asp Gly Ala Ala Ala
35 40 45Asp Gly Asp Leu Ser Thr Glu Asp His Arg His Phe Ser Leu Phe Lys
50 55 60Arg Arg Phe Gly Lys Ser Tyr Ala Ser Gln Glu Glu His Asp His Arg65 70 75 80Phe Ala Val Phe Arg Ala Asn Leu Arg Arg Ala Arg Arg His Gln Glu
85 90 95Leu Asp Pro Ser Ala Val His Gly Val Thr Arg Phe Ser Asp Leu Thr
100 105 110Pro Ser Glu Phe Arg Arg Ser His Leu Gly Ile Arg Gly Gly Leu Arg
115 120 125Leu Pro Lys Asp Ala Asn Glu Ala Pro Leu Leu Pro Thr Asp Asp Leu
130 135 140Pro Glu Asp Phe Asp Trp Arg Asp His Gly Ala Val Thr Gly Val Lys145 150 155 160Asn Gln Gly Ser Cys Gly Ser Cys Trp Ser Phe Ser Ala Thr Gly Ala
165 170 175Leu Glu Gly Ala His Tyr Leu Ala Thr Gly Glu Leu Val Ser Leu Ser
180 185 190Glu Gln Gln Leu Val Asp Cys Asp His Glu Cys Asp Pro Asp Glu Pro
195 200 205Gly Ser Cys Asp Ser Gly Cys Asn Gly Gly Leu Met Asn Ser Ala Phe
210 215 220Glu Tyr Thr Leu Lys Ala Gly Gly Leu Met Arg Glu Gly Asp Tyr Pro225 230 235 240Tyr Thr Gly Thr Asp Arg Gly Thr Cys Lys Phe Asp Lys Ser Lys Ile
245 250 255Ala Ala Ser Val Ser Asn Phe Ser Val Val Ser Leu Asn Glu Asp Gln
260 265 270Ile Ala Ala Asn Leu Val
275
<210>166
<211>96
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>166Ser Leu Ser Glu Gln Glu Leu Ile Asp Cys Asp Thr Thr Tyr Asn Asn1 5 10 15Gly Cys Asn Gly Gly Leu Met Asp Tyr Ala Phe Ser Tyr Ile Ile Ser
20 25 30Asn Gly Gly Leu His Lys Glu Glu Asp Tyr Pro Tyr Ile Met Glu Glu
35 40 45Gly Thr Cys Glu Met Thr Lys Asp Gln Ser Glu Val Val Thr Ile Thr
50 55 60Gly Tyr Lys Asp Val Pro Val Asp Asn Glu Gln Gly Leu Leu Lys Ala65 70 75 80Leu Ala Asn Gln Pro Leu Ser Val Ala Ile Glu Ala Ser Gly Arg Asp
85 90 95
<210>167
<211>148
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>167Lys Met Thr Leu Glu Ala Ala Ser Asn Leu Pro Thr Ser Cys Ser Pro1 5 10 15Arg Leu Leu Gln Phe Ser Asp Leu Thr Pro Ser Glu Phe Arg Arg Thr
20 25 30His Leu Gly Leu Arg Arg Lys Val Lys Leu Pro Lys Asp Ala Asn Glu
35 40 45Ala Pro Ile Leu Pro Thr Gln Asp Leu Pro Lys Asp Phe Asp Trp Arg
50 55 60Asp His Gly Ala Val Thr Ala Val Lys Asn Gln Gly Ser Cys Gly Ser65 70 75 80Cys Trp Ser Phe Ser Thr Thr Gly Ala Leu Glu Gly Ala Asn Tyr Leu
85 90 95Ala Thr Gly Lys Leu Val Ser Leu Ser Glu Gln Gln Leu Val Asp Cys
100 105 110Asp His Glu Cys Asp Pro Glu Glu Pro Gly Ser Cys Asp Ser Gly Cys
115 120 125Asn Gly Gly Leu Met Asn Ser Ala Phe Glu Tyr Thr Leu Ser Thr Gly
130 135 140Val Trp Val Val145
<210>168
<211>119
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>168Pro Thr Arg Val Leu Ser Ser Val Asp Val Lys Pro Phe Lys Tyr Ala1 5 10 15Asn Phe Thr Ala Ile Pro Ala Ala Leu Asp Trp Arg Thr Lys Lys Ala
20 25 30Val Thr Ser Val Lys Asp Gln Gly Val Cys Gly Cys Cys Trp Ala Phe
35 40 45Ser Ala Val Ala Ala Met Glu Gly Leu Thr Gln Leu Lys Lys Arg Lys
50 55 60Leu Val Pro Leu Ser Val Gln Glu Leu Val Asp Cys Asp Val Asn Gly65 70 75 80Lys Asp Lys Gly Cys Arg Gly Gly Tyr Met Asp Ser Ala Phe Glu Phe
85 90 95Val Ile Ser Asn Gly Gly Leu Thr Thr Glu Ala Glu Tyr Pro Tyr Gln
100 105 110Gly Thr Asp Arg Thr Cys Asn
115
<210>169
<211>370
<212>PRT
<213>辐射松(Pinus radiata)
<400>169Asn Cys Ser Thr Met Gly Ser Ser Thr Leu Leu Leu Leu Ala Leu Cys1 5 10 15Ile Ser Ser Val Ile Cys Leu Ser Ser Ala Ile Arg Pro Asp Asp Asp
20 25 30Leu Ile Arg Gln Val Thr Asp Glu Val Asp Ser Asp Pro Gln Ile Leu
35 40 45Asp Ala Arg Ser Ala Leu Phe Asn Ala Glu Ala His Phe Arg Arg Phe
50 55 60Ile Arg Arg Tyr Gly Lys Lys Tyr Ser Gly Pro Glu Glu His Glu His65 70 75 80Arg Phe Gly Val Phe Lys Ser Asn Leu Leu Arg Ala Leu Glu His Gln
85 90 95Lys Leu Asp Pro Gln Ala Ser His Gly Val Thr Glu Phe Ser Asp Leu
100 105 110Thr Gln Glu Glu Phe Arg Arg Gln Tyr Leu Gly Leu Arg Ala Pro Pro
115 120 125Ile Arg Asp Ala His Asp Ala Pro Ile Leu Pro Thr Asn Asp Leu Pro
130 135 140Glu Glu Phe Asp Trp Arg Glu Lys Gly Ala Val Thr Glu Val Lys Asn145 150 155 160Gln Gly Ser Cys Gly Ser Cys Trp Ala Phe Ser Thr Thr Gly Ala Leu
165 170 175Glu Gly Ala Asn Phe Leu Lys Thr Gly Lys Leu Val Ser Leu Ser Glu
180 185 190Gln Gln Leu Val Asp Cys Asp His Glu Cys Asp Pro Ser Asp Ala Arg
195 200 205Ser Cys Asp Ser Gly Cys Asn Gly Gly Leu Met Thr Ser Ala Tyr Gln
210 215 220Tyr Ala Leu Lys Ala Gly Gly Leu Glu Lys Glu Glu Asp Tyr Pro Tyr225 230 235 240Thr Gly Lys Asp Gly Thr Cys Ser Phe Asn Lys Asn Lys Ile Val Ala
245 250 255Gln Val Ser Asn Phe Ser Val Val Ser Ile Asp Glu Asp Gln Ile Ala
260 265 270Ala Asn Leu Val Lys Asn Gly Pro Leu Ser Val Gly Ile Asn Ala Ala
275 280 285Phe Met Gln Thr Tyr Val Gly Gly Val Ser Cys Pro Tyr Ile Cys Ser
290 295 300Lys Arg Met Leu Asp His Gly Val Leu Leu Val Gly Tyr Gly Ser Ala305 310 315 320Gly Phe Ala Pro Ile Arg Met Lys Asp Lys Pro Tyr Trp Ile Ile Lys
325 330 335Asn Ser Trp Gly Pro Asn Trp Gly Glu Asn Gly Phe Tyr Lys Leu Cys
340 345 350Arg Gly His Asn Val Cys Gly Ile Asn Asn Met Val Ser Thr Val Ala
355 360 365Ala Ile
370
<210>170
<211>137
<212>PRT
<213>辐射松(Pinus radiata)
<400>170Ser Thr Met Gly Ser Ser Thr Leu Leu Leu Leu Ala Leu Cys Ile Ser1 5 10 15Ser Val Ile Cys Leu Ser Ser Ala Ile Arg Pro Asp Asp Asp Leu Ile
20 25 30Arg Gln Val Thr Asp Glu Val Asp Ser Asp Pro Gln Ile Leu Asp Ala
35 40 45Arg Ser Ala Leu Phe Asn Ala Glu Ala His Phe Arg Arg Phe Ile Arg
50 55 60Arg Tyr Asp Lys Lys Tyr Ser Gly Pro Glu Glu His Glu His Arg Phe65 70 75 80Gly Val Phe Lys Ser Asn Leu Leu Arg Ala Leu Glu His Gln Lys Leu
85 90 95Asp Pro Gln Ala Ser His Gly Val Thr Glu Phe Ser Asp Leu Thr Gln
100 105 110Glu Glu Phe Arg Arg Gln Tyr Leu Gly Leu Arg Ala Pro Pro Ile Arg
115 120 125Asp Ala His Asp Ala Pro Ile Leu Pro
130 135
<210>171
<211>158
<212>PRT
<213>辐射松(Pinus radiata)
<400>171Asn Pro Lys Ser Leu Glu Phe Ala Glu Phe Ala Val Arg Tyr Gly Lys1 5 10 15Arg Tyr Asp Ser Val His Gln Leu Val His Arg Phe Asn Val Phe Val
20 25 30Lys Asn Val Glu Leu Ile Glu Ser Arg Asn Arg Met Lys Leu Pro Tyr
35 40 45Thr Leu Ala Ile Asn Glu Phe Ala Asp Ile Thr Trp Glu Glu Phe His
50 55 60Gly Gln Tyr Leu Gly Ala Ser Gln Asn Cys Ser Ala Thr His Ser Asn65 70 75 80His Lys Leu Thr Tyr Ala Gln Leu Pro Ala Lys Lys Asp Trp Arg Gln
85 90 95Glu Gly Ile Val Ser Pro Val Lys Asn Gln Ala His Cys Gly Ser Cys
100 105 110Trp Thr Phe Ser Thr Thr Gly Ala Leu Glu Ala Ala Tyr Thr Gln Ala
115 120 125Thr Gly Lys Thr Val Ile Leu Ser Glu Gln Gln Leu Val Asp Cys Ala
130 135 140Gly Ala Phe Asn Asn Phe Gly Cys Asn Gly Gly Leu Pro Ser145 150 155
<210>172
<211>132
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>172Ser Gly Gly Glu Met Asp Thr Ala Phe Ser Phe Ile Gln Arg Asn Gly1 5 10 15Gly Ile Thr Ser Glu Ser Asp Tyr Pro Tyr Arg Gly Arg Asp Gly Ser
20 25 30Cys Asp Ala Ala Met Leu Arg Ser His Ala Ala Thr Ile Ser Gly Tyr
35 40 45Gly Asp Val Pro Pro Asn Asp Glu Arg Ser Leu Gln Ala Ala Val Ala
50 55 60Arg Gln Pro Ile Ser Val Ala Ile Asp Ala Gly Gly Leu Glu Phe Gln65 70 75 80Leu Tyr Ser Arg Gly Ile Phe Thr Gly Ile Cys Gly Tyr Asp Leu Asn
85 90 95His Gly Val Ala Ala Val Gly Tyr Gly Ser Glu Gly Ser Arg Asn Tyr
100 105 110Trp Ile Val Lys Asn Ser Trp Gly Arg Asp Trp Gly Glu Asp Gly Tyr
115 120 125Val Arg Met Leu
130
<210>173
<211>147
<212>PRT
<213>辐射松(Pinus radiata)
<400>173Thr Tyr Lys Leu Gly Leu Asn Lys Phe Ala Asp Leu Ser Asn Glu Glu1 5 10 15Phe Lys Ala Met His Met Thr Thr Thr Met Glu Asn His Arg Ser Leu
20 25 30Arg Arg Asp Arg Gly Val Gln Ser Gly Ser Phe Met Tyr Gln Asn Ser
35 40 45Lys His Leu Pro Ala Ser Ile Asp Trp Arg Lys Lys Gly Ala Val Thr
50 55 60Pro Val Lys Ser Gln Gly Gln Cys Gly Ser Cys Trp Ala Phe Ser Thr65 70 75 80Val Ala Ser Val Glu Gly Ile Asn Tyr Ile Lys Thr Gly Lys Leu Val
85 90 95Ser Leu Ser Glu His Arg Leu Val Asp Cys Ser Lys Glu Asn Ala Gly
100 105 110Cys Asn Gly Gly Leu Met Asp Asn Ala Phe Gln Tyr Ile Ile Asp Asn
115 120 125Gly Gly Ile Val Ser Glu Ala Glu Tyr Pro Tyr Thr Ala Glu Ala Ser
130 135 140Glu Cys Ser145
<210>174
<211>123
<212>PRT
<213>辐射松(Pinus radiata)
<400>174Met Ala Ser Val Ser Lys Ala Thr Leu Leu Leu Phe Leu Ala Thr Thr1 5 10 15Leu Trp Thr Leu Ser Ala His Ala Ser Asp Ser Ser Pro Gly Phe Thr
20 25 30Asp Glu Asp Leu Lys Ser Glu Glu Ser Leu Arg Leu Leu Tyr Asp Lys
35 40 45Trp Ala Leu Arg His Arg Thr Thr Arg Ser Leu Asp Ser Asp Glu His
50 55 60Ala Lys Arg Phe Glu Ile Phe Lys Asp Asn Val Lys Tyr Ile Asp Ser65 70 75 80Val Asn Gln Lys Asp Gly Pro Tyr Lys Leu Gly Leu Asn Lys Phe Thr
85 90 95Asp Leu Ser Asn Glu Glu Phe Lys Ala Met His Met Thr Thr Arg Met
100 105 110Glu Lys His Lys Ser Leu Arg Arg Asp Arg Gly
115 120
<210>175
<211>123
<212>PRT
<213>辐射松(Pinus radiata)
<400>175Met Ala Ser Val Ser Lys Ala Thr Leu Leu Leu Phe Leu Ala Thr Thr1 5 10 15Leu Trp Thr Leu Ser Ala His Ala Ser Asp Ser Ser Pro Gly Phe Thr
20 25 30Asp Glu Asp Leu Lys Ser Glu Glu Ser Leu Arg Leu Leu Tyr Asp Lys
35 40 45Trp Ala Leu Arg His Arg Thr Thr Arg Ser Leu Asp Ser Asp Glu His
50 55 60Ala Lys Arg Phe Glu Ile Phe Lys Asp Asn Val Lys Tyr Ile Asp Ser65 70 75 80Val Asn Gln Lys Asp Gly Pro Tyr Lys Leu Gly Leu Asn Lys Phe Thr
85 90 95Asp Leu Ser Asn Glu Glu Phe Lys Ala Met His Met Thr Thr Arg Met
100 105 110Glu Lys His Lys Ser Leu Arg Arg Asp Arg Gly
115 120
<210>176
<211>127
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>176Glu Ser Ile His Pro Pro Ser Ser Leu Ser Ile Ser Asn Lys Leu Leu1 5 10 15His Gln Lys Leu Asp Pro Ser Ala Ala His Gly Val Thr Gln Phe Ser
20 25 30Asp Leu Thr Pro Ser Glu Phe Arg Arg Thr His Leu Gly Leu Arg Arg
35 40 45Lys Val Lys Leu Pro Lys Asp Ala Asn Glu Ala Pro Ile Leu Pro Thr
50 55 60Gln Asp Leu Pro Lys Asp Phe Asp Trp Arg Asp His Gly Ala Val Thr65 70 75 80Ala Val Lys Asn Gln Gly Ser Cys Gly Ser Cys Trp Ser Phe Ser Thr
85 90 95Thr Gly Ala Leu Glu Gly Ala Asn Tyr Leu Ala Thr Gly Lys Leu Val
100 105 110Ser Leu Ser Glu Gln Gln Leu Val Asp Cys Asp His Glu Cys Asp
115 120 125
<210>177
<211>133
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>177Glu Glu Pro Gln Arg Ser Tyr Glu Val Met Ala Ser Ser Ser Ser Lys1 5 10 15Gly Pro Lys Arg Ser Tyr Asp Ala Met Ala Ser Ser Ser Ser Lys Lys
20 25 30Pro Arg Arg Ser Tyr Asp Val Phe Leu Ser Phe Arg Gly Pro Asp Val
35 40 45Arg Asn His Phe Leu Ser His Leu Tyr Val Ala Leu Asp Gln Ala Gly
50 55 60Ile Ser Thr Tyr Ile Asp Lys Lys Glu Leu Gly Lys Gly Glu Gln Ile65 70 75 80Ser Pro Ala Leu Met Lys Ala Ile Glu Glu Ser His Ile Ala Ile Val
85 90 95Val Phe Ser Glu Asp Tyr Ala Ser Ser Ser Trp Cys Leu Glu Glu Leu
100 105 110Thr Lys Ile Met Glu Cys Lys Glu Gln Lys Gly Leu Met Val Phe Pro
115 120 125Val Phe Tyr Lys Val
130
<210>178
<211>137
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>178Glu Glu Pro Gln Arg Ser Tyr Glu Val Met Ala Ser Ser Pro Ser Lys1 5 10 15Glu Pro Lys Arg Ser Tyr Asp Val Phe Leu Ser Phe Arg Gly Pro Asp
20 25 30Val Arg Asn His Phe Leu Ser His Leu Tyr Ala Ala Leu Asp Gln Val
35 40 45Gly Ile Ser Thr Tyr Ile Asp Asn Glu Glu Leu Arg Lys Gly Glu Gln
50 55 60Ile Ser Pro Ala Leu Met Lys Ala Ile Glu Glu Ser Gln Ile Ala Ile65 70 75 80Val Val Phe Ser Glu Asn Tyr Ala Ser Ser Thr Trp Cys Leu Glu Glu
85 90 95Ile Ser Lys Ile Met Glu Cys Lys Glu Lys Lys Gly Leu Lys Val Leu
100 105 110Pro Val Phe Tyr Lys Val Glu Pro Arg Glu Val Arg Gly Gln Lys Gln
115 120 125Ser Tyr Gly Lys Ala Met Asp Glu His
130 135
<210>179
<211>608
<212>PRT
<213>辐射松(Pinus radiata)
<400>179Tyr Thr His Lys Asn Ser Lys Gly Ala Ala Gly Lys Leu Leu Ala Leu1 5 10 15Gln Asn Leu Ser Asp Ala Asn Ile Glu Ser His Arg Thr Phe Ser Ile
20 25 30Ser Ala Lys Tyr Leu Arg Ser Tyr Phe Ser Val Ile Leu Tyr Pro Met
35 40 45Ala Asn Val Asn Phe Thr Pro Ala Ala Arg Asp Gly Thr Ser Ser Ala
50 55 60Ser Thr Ser Gln Gly Asn Thr Asn Ser Tyr Val Tyr Gln Val Phe Leu65 70 75 80Asn His Arg Gly Pro Asp Val Lys Lys Gly Leu Ala Thr His Ile Tyr
85 90 95His Arg Leu Lys Asp Leu Gly Leu Ser Val Phe Leu Asp Gln Gln Glu
100 105 110Leu Gln Arg Gly Glu Lys Leu Glu Pro Gln Ile Glu Gly Ala Ile Arg
115 120 125Thr Ala Ser Val His Val Ala Ile Phe Ser Pro Asn Tyr Ala Gln Ser
130 135 140Arg Trp Cys Leu Asp Glu Leu Val Gln Met Leu Glu Met Leu Glu Ser145 150 155 160Gly Ser Thr Ile Ile Pro Val Phe Tyr Lys Val Asp Pro Ala Asp Leu
165 170 175Arg Trp Thr Arg Gly Gly Lys Gly Val Tyr Ala Arg Asp Leu Gly Glu
180 185 190Leu Glu Arg Lys Arg Ala Ser Asp Ser Gln Glu Pro Arg Tyr Asp Pro
195 200 205Glu Thr Ile Glu Lys Trp Arg Asn Ala Leu Ser Ala Val Ala Asp Ile
210 215 220Val Gly Phe Glu Leu Lys Asp Lys Glu Glu Ser Gln Leu Val Gln Glu225 230 235 240Val Val Gln Gln Val Val Lys Lys Val Arg Lys Pro Pro Leu Asn Val
245 250 255Ala Lys Tyr Pro Thr Gly Leu Asp Glu Lys Ile Glu Asp Val Asp Arg
260 265 270Thr Leu Ser Leu Gln Arg Gln Ser Glu Lys Ala Thr Ile Leu Gly Ile
275 280 285Val Gly Phe Gly Gly Val Gly Lys Ser Thr Leu Ala Lys Gln Phe Phe
290 295 300Asn Arg Glu Arg Ser Asn Tyr Asp Arg Ser Cys Phe Leu Ser Asp Ile305 310 315 320Arg Ser Lys Ser Leu Pro Ser Val Gln Ser Ser Leu Leu Lys Asp Leu
325 330 335Ile Gln Ser Asp Ala Gln Ile Asn Ser Val Ala Glu Gly Ile Glu Lys
340 345 350Leu Lys Arg Val Ser Gln Arg Cys Leu Ile Ile Leu Asp Asp Ile Asp
355 360 365His Ile Asp Gln Met Asp Ala Leu Tyr Ala Pro Val Ile Arg Ser Ile
370 375 380His Val Gly Ser Leu Ile Leu Ile Thr Ser Arg Asn Lys Asp Val Leu385 390 395 400Arg Ser Ala Gly Ile Gly Glu Ser Ser Ile Cys Thr Leu Lys Gly Leu
405 410 415Asn Gly Glu His Ser Gln Glu Leu Phe Cys Trp His Ala Phe Gly Arg
420 425 430Pro Ser Pro Val Val Gly Phe Glu Lys Val Val Glu Lys Phe Leu Asn
435 440 445Ala Cys Asn Gly Leu Pro Leu Ser Leu Lys Val Leu Gly Ala Leu Leu
450 455 460His Gly Lys Asp Asp Leu Lys Leu Trp Asn Ala Gln Leu Arg Lys Thr465 470 475 480Ser Lys Val Leu Pro Glu Asp Ile Arg Ser Thr Leu Arg Ile Ser Tyr
485 490 495Asp Ala Leu Asp Lys Glu Glu Lys Gln Ile Phe Leu Asp Ile Ala Cys
500 505 510Phe Phe Ile Gly Lys Asn Arg Asp Ser Ala Ile Arg Val Trp Asp Gly
515 520 525Ser Asn Trp Glu Gly Leu Leu Gly Leu Trp Lys Leu Glu Asn Arg Cys
530 535 540Leu Val Glu Val Asp Ser Ser Asn Cys Leu Arg Met His Asp His Leu545 550 555 560Arg Asp Ile Gly Arg Gly Ile Ala Glu Tyr Leu Glu Tyr Pro Arg Arg
565 570 575Leu Trp His Phe Glu Glu Asn Phe Leu Val Ser Ile Leu Lys Leu Ser
580 585 590Thr Asp Phe Gln Asn Ser Pro Leu Leu Ser Lys Leu Ser Val Asn Phe
595 600 605
<210>180
<211>181
<212>PRT
<213>辐射松(Pinus radiata)
<400>180Ala Ser Asp Ser Gln Lys Ser Arg Tyr Asp Thr Asp Thr Ile Glu Lys1 5 10 15Trp Arg Asn Ala Leu Ser Ser Val Ala Asp Ile Val Gly Phe Glu Leu
20 25 30Lys Asp Lys Glu Glu Ser Gln Leu Val Gln Glu Val Val Gln Gln Val
35 40 45Val Lys Lys Phe Pro Lys Pro Pro Leu Asp Val Ala Lys Tyr Pro Thr
50 55 60Gly Leu Asp Glu Lys Ile Lys Asp Val Asp Arg Thr Leu Ser Leu Gln65 70 75 80Arg Gln Ser Glu Lys Ala Thr Ile Leu Gly Ile Val Gly Phe Gly Gly
85 90 95Val Gly Lys Ser Thr Leu Ala Lys Gln Phe Phe Asn Arg Glu Arg Ser
100 105 110Asn Tyr Asp Arg Ser Cys Phe Leu Phe Asp Ile Arg Ser Lys Ser Leu
115 120 125Pro Ser Val Gln Ser Ser Leu Leu Thr Asp Leu Ile Gln Pro Asn Ala
130 135 140Gln Ile Asn Asn Val Asp Glu Gly Ile Glu Arg Leu Lys Thr Val Ser145 150 155 160Gln Arg Cys Leu Ile Ile Leu Asp Asp Ile Asp His Ile Asp Gln Met
165 170 175Asp Ala Leu Tyr Ala
180
<210>181
<211>132
<212>PRT
<213>辐射松(Pinus radiata)
<400>181Ile Asp Gln Leu Asp Ala Leu Cys Ala Pro Val Ile Asp Thr Ile Asp1 5 10 15Val Gly Ser Leu Ile Leu Ile Thr Ser Arg Asn Lys Asp Val Leu Arg
20 25 30Ser Ala Gly Ile Gly Glu Ser Ser Ile Tyr Thr Leu Lys Gly Leu Asn
35 40 45Gly Lys His Ser Gln Glu Leu Phe Cys Trp His Ala Phe Gly Gln Pro
50 55 60Ser Pro Val Val Gly Phe Glu Lys Val Val Glu Lys Phe Leu Asn Val65 70 75 80Cys His Gly Leu Pro Leu Ser Leu Lys Val Phe Gly Ala Leu Leu Arg
85 90 95Gly Lys Asp Asp Leu Glu Leu Trp Asn Ala Glu Leu Arg Lys Thr Ser
100 105 110Lys Val Leu Pro Lys Asp Ile Arg Ser Thr Leu Arg Ile Ser Tyr Asp
115 120 125Ala Leu Asp Lys
130
<210>182
<211>395
<212>PRT
<213>辐射松(Pinus radiata)
<400>182Ile Gly Ile Asp Val Cys Phe Ser Leu Gly Leu Leu Asn Phe Ser Ser1 5 10 15Gln Asp Leu Val Leu Glu Leu Leu Asp Lys Val Val Lys His Leu Leu
20 25 30Glu Leu Val Pro Lys Pro Asp Leu Tyr Val Ala Glu Tyr Pro Thr Gly
35 40 45Leu Asp Asp Lys Leu Lys Asp Phe Glu Asp Thr Val Leu Leu Arg Gln
50 55 60Gln Gln Gly Arg Lys Pro Gln Ile Leu Gly Ile Val Gly Leu Gly Gly65 70 75 80Val Gly Lys Thr Thr Leu Ala Thr Ala Phe Phe Asn Lys Lys Lys Ser
85 90 95Ala Tyr His Arg Ser Cys Phe Leu Cys Asp Val Arg Glu Asn Thr Thr
100 105 110Asn Arg Ser Leu His Leu Leu Gln Ser Gln Leu Leu Asn Ser Leu Thr
115 120 125Gly Phe Asn Asn Gln Val Asn Ser Glu Arg Glu Gly Lys Gly Met Leu
130 135 140Ile Glu Pro Leu Lys Ser Cys Lys Ala Ile Met Ile Phe Asp Asp Val145 150 155 160Asp Asp Val Asp Gln Val Lys Ala Phe Leu Pro Gln Ser Asp Val Leu
165 170 175Asn Ser Glu Ser Leu Ile Leu Ile Thr Thr Arg Asp Arg Asn Val Leu
180 185 190Arg Ser Leu Lys Val Glu Asn Ser Ser Ile Tyr Ser Leu Ser Gly Leu
195 200 205Asn Lys Glu His Ser Leu Glu Leu Phe Cys Ser His Ala Phe Ser Pro
210 215 220Ala Phe Pro Leu Pro Glu Phe Lys Ser Leu Val Asp Lys Phe Ile Asp225 230 235 240Tyr Cys Asn Gly Leu Pro Leu Ser Leu Lys Ile Phe Gly Ala Leu Leu
245 250 255Tyr Gly Lys Asp Ile Ser Gln Trp Lys Glu Glu Trp Glu Ser Leu Arg
260 265 270Gln Ile Ala Pro Ile Ala Ile His Asp Thr Phe Lys Ile Ser Tyr Asp
275 280 285Ser Leu Asn Gln Glu Glu Lys Asp Ile Phe Leu Asp Ile Ala Cys Phe
290 295 300Leu Arg Cys His His Arg Asp Ala Ala Ile Ser Ile Trp Asn Lys Ser305 310 315 320Gly Trp Arg Gly Asn Arg Gly Phe Leu Asn Leu Gln Asp Lys Ser Leu
325 330 335Val Glu Val Asp Ala Phe Asn Cys Ile Gln Met His Asn His Leu Arg
340 345 350Asp Leu Gly Arg Gln Val Ala Ala Ser Ser Leu Pro Pro Arg Leu Leu
355 360 365Ile Thr Lys Asn Leu Ile His Asn Leu Ser His Gln Ser Ser Val Ser
370 375 380Val Gln Ser Phe Asn Pro Leu Phe Val Ile His385 390 395
<210>183
<211>523
<212>PRT
<213>辐射松(Pinus radiata)
<400>183Asn Val Leu Asp Leu Ala Cys Phe Phe Leu Leu Leu Cys Pro Met Ala1 5 10 15Asp His Thr Gly Asp Ile Thr Cys Ile Ala Ser Ser Ser Ser Ser Ser
20 25 30Thr Asn Thr Gly Gln Val Phe Asp Val Phe Leu Asn His Arg Gly Pro
35 40 45Asp Thr Lys Lys Gly Leu Ala Ser His Ile Tyr Arg Gly Leu Ile Val
50 55 60Arg Gly Leu Arg Val Phe Leu Asp Gln Pro Glu Leu Arg Lys Gly Glu65 70 75 80Asp Asn Leu Ser Gln Ile Lys Glu Ala Ile Arg Thr Ala Ser Val His
85 90 95Val Ala Ile Phe Ser Pro Asn Tyr Ala Gln Ser Arg Trp Cys Leu Asp
100 105 110Glu Leu Ala Leu Met Val Glu Ser Glu Ser Thr Ile Ile Pro Val Phe
115 120 125His Asp Val Asp Pro Ser Glu Leu Arg Trp Gln Gln Ser Gly Asp Gly
130 135 140Val Glu Ser Ile Ile Arg Cys Leu Cys Pro Cys Leu Leu Gly Gly Lys145 150 155 160Gly Arg Tyr Ala Arg Asp Leu His Met Leu Gln Lys Lys Thr Thr Leu
165 170 175Asp Pro His Thr Asn Lys Lys Lys Pro Arg His Asp Ser Arg Thr Leu
180 185 190Gln Lys Trp Arg Lys Ala Leu Ser Asp Val Ser Asn Lys Ser Gly Phe
195 200 205Ile Ile Asn Ala Tyr Asn Gly Asp Glu Gly Gln Leu Val Asp Ala Val
210 215 220Val Glu Glu Val Trp Arg Lys Val Glu Lys Thr Pro Leu Asn Val Ala225 230 235 240Lys Tyr Pro Thr Gly Leu Val Glu Lys Ile Glu Asp Val Gly Arg Met
245 250 255Val Leu Leu Gln His Gln Ser Gln Lys Thr Lys Val Val Gly Ile Val
260 265 270Gly Leu Gly Gly Val Gly Lys Thr Thr Leu Ala Lys Glu Phe Phe Asn
275 280 285Arg His Arg Ser Asn Tyr Asp Arg Ser Cys Phe Leu Phe Asp Val Arg
290 295 300Glu Thr Ala Ala Lys Ser Ser Leu Ser Ser Leu Gln Thr Gln Leu Leu305 310 315 320Lys His Leu Ala His Leu Gln Asp Glu Gln Ile Arg Asn Thr Asp Glu
325 330 335Val Ile Glu Lys Leu Arg Lys His Leu Ser Ser Ser Pro Arg Ser Leu
340 345 350Ile Val Leu Asp Asp Val Asp His Ile Asp Gln Leu Asp Ala Leu Phe
355 360 365Ser Pro Val Ile Asp Thr Ile Gln Ala Ser Ser Leu Ile Leu Val Thr
370 375 380Ser Arg Asn Arg Asp Val Leu Ile Ser Ser Gly Ile Leu Glu Ala Ser385 390 395 400Ile Tyr Gln Gln Thr Gly Leu Asn Pro Gln Gln Ser Arg Glu Leu Phe
405 410 415Cys Ser His Ala Phe Asp Gln Ser Cys Pro Val Thr Gly Phe Glu Gln
420 425 430Leu Val Glu Asp Phe Leu Asp Phe Cys Asp Gly Leu Pro Leu Ser Leu
435 440 445Lys Val Ile Gly Ala Ala Ile Arg Gly Lys Asp Ser Glu Phe Trp Ile
450 455 460Gly Gln Leu Asp Lys Asn Arg Arg Ile Leu His Thr Asp Ile His Ser465 470 475 480Lys Leu Lys Ile Ser Tyr Asp Gly Leu Asp Lys Glu Glu Gln Gln Ile
485 490 495Phe Leu Asp Val Ala Cys Phe Phe Ile Gly Glu Asn Arg Asp Thr Ala
500 505 510Ile Arg Arg Trp Asn Gly Ser Asp Gly Lys Cys
515 520
<210>184
<211>115
<212>PRT
<213>辐射松(Pinus radiata)
<400>184Ser His Ile Tyr Arg Gly Leu Ile Val Arg Gly Leu Arg Val Phe Leu1 5 10 15Asp Gln Pro Glu Leu Arg Lys Gly Lys Asp Ile Pro Ser Gln Ile Lys
20 25 30Glu Ala Ile Arg Thr Asp Ser Val His Val Ala Ile Phe Ser Pro Thr
35 40 45Tyr Ala Gln Ser Arg Trp Cys Leu Asp Glu Leu Ala Leu Met Val Glu
50 55 60Ser Lys Ser Thr Ile Ile Pro Val Phe His Asp Val Asp Pro Phe Glu65 70 75 80Leu Arg Trp Pro Gln Ser Gly Asp Gly Val Glu Ser Ile Ile Arg Cys
85 90 95Leu Cys Pro Cys Leu Leu Gly Gly Lys Gly Arg Tyr Ala Arg Asp Leu
100 105 110His Met Leu
115
<210>185
<211>579
<212>PRT
<213>辐射松(Pinus radiata)
<400>185Leu Val Glu Ile Asp Ser Glu Gly Cys Ile Arg Met His Asp His Leu1 5 10 15Arg Asp Leu Gly Arg Asp Val Ala Glu Lys Glu His Pro Leu Arg Leu
20 25 30Ser Arg Pro Asn Val Asn Leu Leu Arg Thr Leu Ser Pro Ser Ser Pro
35 40 45Val Arg Gly Ile Ser Met Asn Tyr Gly Asn Gly Gly Lys Gln Phe Leu
50 55 60Glu Tyr Ile Arg Val Asn Cys Asn Leu Ser Arg Leu Glu Leu Leu Arg65 70 75 80Gly Glu Gly Ser Phe Val Glu Ser Ile Phe Ser Ala Gly Glu Ile Arg
85 90 95Gln Leu Val Tyr Leu Gln Trp Lys Glu Cys Pro Ile Ser Ser Ile Ser
100 105 110Phe Thr Ile Pro Thr Arg Asn Leu Ser Val Leu Tyr Ile Gln Gly Tyr
115 120 125Ala Leu Lys Thr Leu Trp Gln His Glu Ser Gln Ala Pro Leu Gln Leu
130 135 140Thr Glu Leu Tyr Ile Asp Ala Thr Leu Ser Glu Val Pro Gln Ser Ile145 150 155 160Gly Lys Leu Asn Gln Leu Glu Arg Ile Val Leu Lys Asn Gly Tyr Phe
165 170 175Lys Thr Leu Pro Asn Glu Phe Tyr Asp Met His Ser Leu Lys His Ile
180 185 190Thr Leu Gln Asn Cys Glu Gln Met Met Leu Leu Pro Asp Ser Val Gly
195 200 205Ile Leu Thr Gly Arg Gln Thr His Asp Phe Ser Gly Cys Ser Asn Leu
210 215 220Gln Ala Leu Pro Asp Ser Val Gly Gln Leu Thr Gly Leu Lys Thr Leu225 230 235 240Asp Leu Glu Asp Cys Thr Ser Leu Gln Gly Leu Pro Asp Ser Val Gly
245 250 255Gln Leu Thr Gly Leu Gln Ser Leu Asp Leu Glu His Cys Thr Ser Leu
260 265 270Gln Gly Leu Pro Asp Ser Val Gly Gln Leu Thr Gly Leu Gln Thr Leu
275 280 285Asp Leu Arg Gly Cys Ser Ser Leu Gln Gly Leu Pro Asp Ser Val Gly
290 295 300Gln Leu Thr Gly Leu Glu Gly Leu Tyr Leu Ser Gly Cys Phe Ser Leu305 310 315 320Gln Gly Leu Pro Asp Ser Val Glu Gln Leu Thr Gly Leu Glu Gly Leu
325 330 335Tyr Leu Ser Gly Cys Phe Ser Leu Gln Gly Leu Pro Asp Ser Val Gly
340 345 350Gln Leu Thr Gly Leu Gln Ser Leu Asn Leu Glu Tyr Cys Thr Ser Leu
355 360 365Glu Gly Leu Pro Asp Ser Val Gly Gln Leu Thr Asp Leu Pro Ile Leu
370 375 380Asp Leu Asn Thr Cys Ile Ser Leu Gln Gly Leu Pro Asp Ser Val Gly385 390 395 400Gln Leu Arg Gly Leu Gln Asn Leu Asp Leu Arg Trp Cys Asp Ser Leu
405 410 415Gln Gly Leu Pro Asp Ser Val Gly Gln Leu Thr Gly Leu Gln Ile Leu
420 425 430Asp Leu Ser Gly Cys Thr Ser Leu Gln Gly Leu Pro Asp Ser Val Gly
435 440 445Gln Leu Thr Gly Leu Arg Thr Leu His Leu Glu Asn Cys Thr Ser Leu
450 455 460Gln Gly Leu Pro Asp Ser Val Gly Asn Leu Thr Ser Leu Lys Trp Leu465 470 475 480Asn Leu Ser Gly Cys Ser Asn Leu Gln Met Leu Pro Asn Phe Arg His
485 490 495Leu Ser Ser Leu Glu Glu Leu His Leu Ser Gly Cys Ser Asn Leu Gln
500 505 510Met Pro Pro Asn Val Gln His Leu Ser Ser Leu Val Glu Leu Ser Val
515 520 525Ser His Cys Ser Lys Leu Gln Trp Gly Ala Gly Val Val Glu Ser Leu
530 535 540Arg His Arg Leu Gly Asn Gly Phe Ile Glu Glu Gly Gly Glu Asn Ile545 550 555 560Asp Lys Glu Ser Trp Glu Glu Gly Ser Glu Lys Ser Asp Lys Glu Ser
565 570 575Trp Glu Glu
<210>186
<211>175
<212>PRT
<213>辐射松(Pinus radiata)
<400>186Val Leu Gln Thr Leu Asp Leu Arg Arg Cys Ser Ser Leu Gln Gly Leu1 5 10 15Pro Glu Ser Val Gly Gln Leu Thr Gly Leu Gln Ser Leu Asn Leu Glu
20 25 30Lys Cys Thr Arg Leu Gln Gly Leu Pro Glu Ser Val Gly Gln Leu Thr
35 40 45Gly Leu Gln Thr Leu Asp Leu Arg Arg Cys Ser Ser Leu Gln Gly Leu
50 55 60Pro Glu Ser Val Gly Gln Leu Thr Gly Leu Gln Ser Leu Asn Leu Lys65 70 75 80Glu Cys Thr Ser Leu Gln Gly Leu Pro Asn Ser Leu Gly Gln Leu Thr
85 90 95Gly Leu His Ser Leu Tyr Leu Val Glu Cys Ser Ser Leu Gln Gly Leu
100 105 110Pro Asp Ser Val Gly Gln Leu Thr Gly Leu Gln Ser Ile Asn Leu Gln
115 120 125Gly Cys Ser Ser Leu Gln Gly Leu Pro Asp Ser Val Gly Gln Leu Thr
130 135 140Gly Leu His Ser Leu Asn Leu Glu Gly Cys Ser Arg Leu Gln Gly Leu145 150 155 160Pro Asp Leu Val Gly Gln Leu Thr Gly Leu Gln Ser Leu Lys Leu
165 170 175
<210>187
<211>161
<212>PRT
<213>辐射松(Pinus radiata)
<400>187Asp Arg Cys Ser Ser Leu Gln Gly Leu Pro Asp Ser Val Gly Gln Leu1 5 10 15Thr Gly Leu Arg Gln Leu Asn Leu Asn Gly Cys Ser Ser Leu Gln Gly
20 25 30Leu Pro Asp Ser Val Gly Gln Leu Thr Gly Leu Trp Ile Leu Asp Leu
35 40 45Thr Gly Cys Ser Ser Leu Gln Gly Leu Pro Asp Ser Val Arg Gln Leu
50 55 60Arg Cys Leu Arg Gly Gly Ser Gly Arg Ala Cys Gly Arg Ala Ala Glu65 70 75 80Ala Pro Ala Tyr Asn Gly Gly Leu Pro Gly Gly Leu Ala Glu Ser Arg
85 90 95Leu Ala Arg Gly Tyr Trp Leu Asn Leu Ser Gly Cys Ser Asn Leu Gln
100 105 110Met Pro Pro Asn Val Gln His Leu Ser Ser Leu Leu Lys Leu Tyr Val
115 120 125Ser His Cys Ser Lys Leu Gln Trp Gly Ala Gly Val Val Glu Ser Leu
130 135 140Arg His Arg Leu Glu Ile Thr Ser Ser Lys Lys Ala Ala Lys Thr Ser145 150 155 160Met
<210>188
<211>305
<212>PRT
<213>辐射松(Pinus radiata)
<400>188Gly Leu Pro Asp Ser Val Gly Gln Leu Thr Gly Leu Gln Thr Leu Asp1 5 10 15Leu Arg Gly Cys Ser Ser Leu Gln Gly Leu Pro Asp Ser Val Gly Gln
20 25 30Leu Thr Gly Leu Glu Gly Leu Tyr Leu Ser Gly Cys Phe Ser Leu Gln
35 40 45Gly Leu Pro Asp Ser Val Glu Gln Leu Thr Gly Leu Glu Gly Leu Tyr
50 55 60Leu Ser Gly Cys Phe Ser Leu Gln Gly Leu Pro Asp Ser Val Gly Gln65 70 75 80Leu Thr Gly Leu Gln Ser Leu Asn Leu Glu Tyr Cys Thr Ser Leu Glu
85 90 95Gly Leu Pro Asp Ser Val Gly Gln Leu Thr Asp Leu Pro Ile Leu Asp
100 105 110Leu Asn Thr Cys Ile Ser Leu Gln Gly Leu Pro Asp Ser Val Gly Gln
115 120 125Leu Arg Gly Leu Gln Asn Leu Asp Leu Arg Trp Cys Asp Ser Leu Gln
130 135 140Gly Leu Pro Asp Ser Val Gly Gln Leu Thr Gly Leu Gln Ile Leu Asp145 150 155 160Leu Ser Gly Cys Thr Ser Leu Gln Gly Leu Pro Asp Ser Val Gly Gln
165 170 175Leu Thr Gly Leu Arg Thr Leu His Leu Glu Asn Cys Thr Ser Leu Gln
180 185 190Gly Leu Pro Asp Ser Val Gly Asn Leu Thr Ser Leu Lys Trp Leu Asn
195 200 205Leu Ser Gly Cys Ser Asn Leu Gln Met Leu Pro Asn Phe Arg His Leu
210 215 220Ser Ser Leu Glu Glu Leu His Leu Ser Gly Cys Ser Asn Leu Gln Met225 230 235 240Pro Pro Asn Val Gln His Leu Ser Ser Leu Val Glu Leu Ser Val Ser
245 250 255His Cys Ser Lys Leu Gln Trp Gly Ala Gly Val Val Glu Ser Leu Arg
260 265 270His Arg Leu Gly Asn Gly Phe Ile Glu Glu Gly Gly Glu Asn Ile Asp
275 280 285Lys Glu Ser Trp Glu Glu Gly Ser Glu Lys Ser Asp Lys Glu Ser Trp
290 295 300Glu305
<210>189
<211>85
<212>PRT
<213>辐射松(Pinus radiata)
<400>189Met Leu Pro His Phe Arg His Leu Ser Leu Met Glu Glu Leu His Leu1 5 10 15Ser Gly Cys Ser Asn Leu Gln Met Pro Pro Asn Val Gln His Leu Ser
20 25 30Ser Leu Val Lys Leu Tyr Val Ser His Cys Ser Lys Leu Gln Trp Gly
35 40 45Ala Gly Val Val Glu Ser Leu Arg His Arg Leu Gly Asn Gly Phe Ile
50 55 60Glu Glu Gly Gly Glu Asn Ser Asn Glu Tyr Asn Cys Ser Glu Leu Tyr65 70 75 80Asn Ile Arg Glu Leu
85
<210>190
<211>320
<212>PRT
<213>辐射松(Pinus radiata)
<400>190Asn Ser Cys Thr Ser Leu Gln Gly Leu Pro Asp Ser Val Gly Gln Leu1 5 10 15Thr Gly Leu Arg Thr Leu Asp Leu Asn Ser Cys Thr Ser Leu Gln Gly
20 25 30Leu Pro Asp Ser Val Gly Gln Leu Thr Gly Leu Arg Thr Leu Asp Leu
35 40 45His Ser Cys Thr Ser Leu Gln Gly Leu Pro Asp Ser Val Gly Gln Leu
50 55 60Thr Gly Leu Glu Thr Leu Asp Leu Gln Asp Cys Thr Ser Leu Gln Gly65 70 75 80Leu Pro Asp Ser Val Gly Gln Leu Thr Gly Leu Gln Val Leu Tyr Leu
85 90 95Arg Arg Cys Ser Asn Leu Gln Gly Leu Pro Asp Ser Val Gly Gln Leu
100 105 110Thr Cys Leu Lys Val Leu Cys Leu Arg Trp Cys Ser Asn Leu Gln Ala
115 120 125Leu Pro Asp Ser Val Gly Gln Leu Thr Gly Leu Lys Ala Leu Asn Leu
130 135 140Gln Asp Cys Thr Ser Leu Gln Gly Leu Pro Asp Leu Val Gly Gln Leu145 150 155 160Thr Gly Leu Lys Ala Leu Asn Leu Gln Asn Cys Thr Ser Leu Gln Gly
165 170 175Leu Pro Asp Ser Val Gly Gln Leu Thr Gly Leu Gln Val Leu Tyr Leu
180 185 190Arg Gln Cys Ser Asn Leu Gln Ala Leu Pro Asp Ser Val Gly Gln Leu
195 200 205Thr Gly Leu Asn Lys Leu Tyr Leu Asn Gly Cys Ser Ser Leu Gln Gly
210 215 220Leu Pro Asp Ser Val Glu Asn Leu Thr Arg Leu Lys Trp Leu Ile Leu225 230 235 240Ser Gly Cys Ser Asn Leu Gln Met Leu Pro Asn Phe Arg His Leu Arg
245 250 255Ser Leu Glu Arg Leu His Leu Ser Gly Cys Ser Asn Leu Gln Met Pro
260 265 270Pro Asn Val Gln His Leu Ser Ser Leu Val Gln Leu Tyr Val Ser His
275 280 285Cys Ser Lys Leu Gln Trp Gly Ala Gly Val Val Glu Ser Leu Arg His
290 295 300Arg Leu Gly Asn Gly Phe Ile Glu Glu Gly Gly Glu Asn Ser Asn Glu305 310 315 320
<210>191
<211>355
<212>PRT
<213>辐射松(Pinus radiata)
<400>191Ile Thr Ser Asp Glu Val Glu Leu Ile Glu Lys Ile Val Gln Ser Val1 5 10 15Leu Glu Lys Val Asn Arg Ser Ser Phe Tyr Val Pro Lys Tyr Glu Val
20 25 30Gly Leu Asp Glu Asn Val Glu Lys Phe Arg Lys Lys Val Lys Glu Trp
35 40 45Ser Gln Gln Arg Gln Asn Glu Lys Ala Gln Val Val Gly Ile Val Gly
50 55 60Leu Gly Asp Val Gly Lys Thr Thr Leu Val Lys Glu Phe Phe Pro Thr65 70 75 80Glu Ser Pro Ala Tyr Arg Asn Phe Cys Phe Tyr Pro Val Arg Arg Asn
85 90 95Gly Cys Ile Thr Arg Pro Asp Cys Leu Ile Gly Glu Leu Phe Lys Gly
100 105 110Ser Ser Gly Leu Val Ser Ser Pro Thr Ser Val Asp Ala Val Lys Ile
115 120 125Leu Pro Asp Ala Ser Asn Pro Ser Val Asp Met Ile Arg Asn Asn Pro
130 135 140Ser Leu Val Val Leu Asp Gly Val Asp Asn Val Glu Glu Arg Glu Asn145 150 155 160Leu Leu Lys Ile Gln Glu Arg Leu His Ser Lys Ser Leu Ile Leu Ile
165 170 175Thr Ser Arg Asp Pro Glu Val Leu Arg Cys Ser Glu Val Glu Lys Ile
180 185 190Tyr His Leu Asn Gly Leu Asn Glu Pro Cys Ser Arg Lys Leu Phe Cys
195 200 205Phe His Ala Phe His Gln Ala Ala Pro Leu Gln Gly Tyr Glu Tyr Leu
210 215 220Val Ala Trp Val Leu Arg Val Cys Asp Gly Leu Pro Leu Leu Leu Lys225 230 235 240Leu Leu Gly Ala Leu Leu Cys Gly Asn Asn Asp Arg Phe Tyr Trp Glu
245 250 255Asp Leu Cys Asp Ser Leu Gln Ala Lys Lys Ile Glu Glu Lys Leu Lys
260 265 270Val Ile Tyr Asp Thr Leu Gly Thr Glu Glu Gln Gln Thr Phe Leu Asp
275 280 285Ile Ala Cys Asn Leu Val Gly Lys Asn Ala Asp Ile Trp Leu Lys Ser
290 295 300Gly Lys Lys Gly Ile Ile Trp Phe Gln Ile Leu Leu Glu Lys Arg Leu305 310 315 320Val Glu Val Asp Ser Glu Asn Cys Ile Gln Met His Asp Leu Leu Lys
325 330 335Asn Leu Gly Gly Glu Ile Ala Lys Ala Thr Lys Ser Pro Arg Pro Leu
340 345 350Phe Phe Gly
355
<210>192
<211>433
<212>DNA
<213>辐射松(Pinus radiata)
<400>192ctgaggaaga aggcgtaagt ttctttaaaa gttttcattt tggtttttat ccccgagctt 60aaatctttga ttttctcctc taaatggtta gcatttgcag agcattgcaa atgtaggaaa 120aagtaagaaa aaaactttca atacagttcg tgcattcttc tactgggtta ctaaagagga 180aggatcattt gattggttca agggagtcat gaatgaagtg gcagaaattg atcaaaaggg 240cataattgag ttgcacaact attgcacaag tgtttatgaa gagggtgatg ctcgatcagc 300attgatcgct atgttgcagg cactaaatca tgccaaacat ggtgttgata ttgtatcggg 360caccagggtt tgcacacact ttgccaagcc taattggaga aacgcttcaa gagtattgct 420ctcatgcaca aag 433
<210>193
<211>431
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>193agacccctta cgtgagaaga gaattaagag aattcacaaa gccttagact ccgatgatat 60tgaacttgtt acgcttctct tgagcgagtc caatatcaac ttagatgaag cctatggctt 120acattatgct gcagcttact gtgaccccaa ggttgtctct gagttgcttg gtttgggctt 180ggctaacgtc aaccttcgga acccaagggg atacactgtg ctccatgttg ctgcaatgag 240gaaggagact aagatcatag tctcattgtt gtcaaaaggt gcttgtgcat cggaattgac 300acctgatgga cagaatgctg tcagcatctg ccgaaggttg acaaggccta aggattataa 360tgctaaaaca gagcagtgcc aggaagcaaa caaggacagg ctatgcatag atgtactgga 420gagggaattg t 431
<210>194
<211>427
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>194tcaataagat catcgatacc ggtgacttgt catggctggg aactgttggt gatgacactc 60cagaggaaca acttcgtaag aagcaaaggc atatggaagt tgaagaacag atgactgaag 120cttatactca agacaaagag gagactgata agtctagtat attgtcttct tcatcgtcga 180cttcaatgag tttccttaag cctaatggta aattaacgac aagtgaccag cagcggcact 240gcgagattta agatgccaaa agacatcttt ctcattgtac aatattccaa ttttttccca 300tgtgatgtat ctttatagcc agtcgtttgt aagtactcat ctacacagca aatagatgag 360gagtgatata cgtacatgca gatgattgtc ttaatagaaa atcatttctc ttaaaaaaaa 420aaaaaaa 427
<210>195
<211>306
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>195cacaggtgct tgttggcggc tcggagccag tttcttcacg agtttttcaa gcagggaggc 60ggcgacaatg caagggaagg aaagccgagg tatcccattt cggacttagt gaaaaagggt 120catgttggat gtgaggcttt taaatatgtg ttgagataca tgtacacggg gaagctcaag 180ctatttccag cggaggtgtc gacatgcgtg gacagcagtt gcgcacatga cgtgtgcggc 240cctgctatta attatgccgt ggagttgatg tatgcctcgg ccacttttga gatagcagag 300ttagtg 306
<210>196
<211>114
<212>PRT
<213>辐射松(Pinus radiata)
<400>196His Leu Gln Ser Ile Ala Asn Val Gly Lys Ser Lys Lys Lys Thr Phe1 5 10 15Asn Thr Val Arg Ala Phe Phe Tyr Trp Val Thr Lys Glu Glu Gly Ser
20 25 30Phe Asp Trp Phe Lys Gly Val Met Asn Glu Val Ala Glu Ile Asp Gln
35 40 45Lys Gly Ile Ile Glu Leu His Asn Tyr Cys Thr Ser Val Tyr Glu Glu
50 55 60Gly Asp Ala Arg Ser Ala Leu Ile Ala Met Leu Gln Ala Leu Asn His65 70 75 80Ala Lys His Gly Val Asp Ile Val Ser Gly Thr Arg Val Cys Thr His
85 90 95Phe Ala Lys Pro Asn Trp Arg Asn Ala Ser Arg Val Leu Leu Ser Cys
100 105 110Thr Lys
<210>197
<211>143
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>197Asp Pro Leu Arg Glu Lys Arg Ile Lys Arg Ile His Lys Ala Leu Asp1 5 10 15Ser Asp Asp Ile Glu Leu Val Thr Leu Leu Leu Ser Glu Ser Asn Ile
20 25 30Asn Leu Asp Glu Ala Tyr Gly Leu His Tyr Ala Ala Ala Tyr Cys Asp
35 40 45Pro Lys Val Val Ser Glu Leu Leu Gly Leu Gly Leu Ala Asn Val Asn
50 55 60Leu Arg Asn Pro Arg Gly Tyr Thr Val Leu His Val Ala Ala Met Arg65 70 75 80Lys Glu Thr Lys Ile Ile Val Ser Leu Leu Ser Lys Gly Ala Cys Ala
85 90 95Ser Glu Leu Thr Pro Asp Gly Gln Asn Ala Val Ser Ile Cys Arg Arg
100 105 110Leu Thr Arg Pro Lys Asp Tyr Asn Ala Lys Thr Glu Gln Cys Gln Glu
115 120 125Ala Asn Lys Asp Arg Leu Cys Ile Asp Val Leu Glu Arg Glu Leu
130 135 140
<210>198
<211>82
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>198Asn Lys Ile Ile Asp Thr Gly Asp Leu Ser Trp Leu Gly Thr Val Gly1 5 10 15Asp Asp Thr Pro Glu Glu Gln Leu Arg Lys Lys Gln Arg His Met Glu
20 25 30Val Glu Glu Gln Met Thr Glu Ala Tyr Thr Gln Asp Lys Glu Glu Thr
35 40 45Asp Lys Ser Ser Ile Leu Ser Ser Ser Ser Ser Thr Ser Met Ser Phe
50 55 60Leu Lys Pro Asn Gly Lys Leu Thr Thr Ser Asp Gln Gln Arg His Cys65 70 75 80Glu Ile
<210>199
<211>102
<212>PRT
<213>巨杉(Eucalyptus grandis)
<400>199His Arg Cys Leu Leu Ala Ala Arg Ser Gln Phe Leu His Glu Phe Phe1 5 10 15Lys Gln Gly Gly Gly Asp Asn Ala Arg Glu Gly Lys Pro Arg Tyr Pro
20 25 30Ile Ser Asp Leu Val Lys Lys Gly His Val Gly Cys Glu Ala Phe Lys
35 40 45Tyr Val Leu Arg Tyr Met Tyr Thr Gly Lys Leu Lys Leu Phe Pro Ala
50 55 60Glu Val Ser Thr Cys Val Asp Ser Ser Cys Ala His Asp Val Cys Gly65 70 75 80Pro Ala Ile Asn Tyr Ala Val Glu Leu Met Tyr Ala Ser Ala Thr Phe
85 90 95Glu Ile Ala Glu Leu Val
100
<210>200
<211>1425
<212>DNA
<213>辐射松(Pinus radiata)
<400>200atcattttgt gtgaaaataa gcgatgggtt ggttccacag gtgttttgga ttcattagaa 60agaagaagaa gcagaaaagc ccaaaatctg agcctccgtc tcgtgaacat ttactgaagt 120ccacacaaga agaattcgag aatacaaagg gagctcaata caaatatcac cgcagatttc 180ctgctgttag ggataaaacc gagcaggttg cgacgcgatc atttggggat ttggatggag 240cttcactaat agaaactcct ggcagagaaa ccctccaaat tgttataaca gagtgcccaa 300atactcgtac agtatgttct ggatgtaaat ccaggttaag caactggtgt ccgtcctgca 360gatgcaacct tggaaatttt aggtgcttag ctcctgaaac ggagacatca tctcaagaac 420ttacttgcat gtatcaaagc tatggttgtg aggatatgta tccttactac agtgaattaa 480gacatgaagc tcactgcaat tttaggccat acaactgtcc ctatgctggc tccgaatgca 540agctagttgg agatattccc tttttggtgg ctcatttaag agatgatcac aaagtttata 600tgcataatag ttgcaccttt gatcatcgat atgtaaagtc aaatccactc gaggttgaga 660atgctatttg gatgccaact gtaatcaatt gttttgggca attcttttgt ctacattttg 720aagcgtttct attagacatg gcccctgtat atatagcttt tctgattttc atgggagatg 780ataatgaagc taaaaacttt agctattgcc tcgagactgg aggcaatggt cggaaactga 840tttggcatgg ggttcctcga agcatcagag attgtcacag gaaagttcat gacagtagtg 900acggactaat tatacaaaga gatgtggcac tctttttctc aggtggtgac ataaatgaat 960tgaatcttag attgacagga cacatattga aggaacaata atatatgcac ttttcaaaga 1020tctatggact aggaaaagta agtcatatct cctgttattt atcttctcct ttgctgctga 1080ttaatattgt aaaggttcag atcctttcag tagcaagctg tcattgccag aacaacgaga 1140gagaaaaatc atatctagaa agtgtatagg ttgaccacgg cacaggtgta tgccattttc 1200tcatgtaaag acattctcct aattgctaaa gaatgtactt gaattgaatt gaatgccctt 1260tatttatgga ttgtctgatc gtaatcatgg agagaatatt tgttgttgtt taccactgcc 1320aacaatacta cgagcgggag aggatttggg tggtagtggt tgtgtaggaa ttaagaatcc 1380ggactcaaag gttttgataa taaggtttga atcttaaaaa aaaaa 1425
<210>201
<211>325
<212>PRT
<213>辐射松(Pinus radiata)
<400>201Met Gly Trp Phe His Arg Cys Phe Gly Phe Ile Arg Lys Lys Lys Lys1 5 10 15Gln Lys Ser Pro Lys Ser Glu Pro Pro Ser Arg Glu His Leu Leu Lys
20 25 30Ser Thr Gln Glu Glu Phe Glu Asn Thr Lys Gly Ala Gln Tyr Lys Tyr
35 40 45His Arg Arg Phe Pro Ala Val Arg Asp Lys Thr Glu Gln Val Ala Thr
50 55 60Arg Ser Phe Gly Asp Leu Asp Gly Ala Ser Leu Ile Glu Thr Pro Gly65 70 75 80Arg Glu Thr Leu Gln Ile Val Ile Thr Glu Cys Pro Asn Thr Arg Thr
85 90 95Val Cys Ser Gly Cys Lys Ser Arg Leu Ser Asn Trp Cys Pro Ser Cys
100 105 110Arg Cys Asn Leu Gly Asn Phe Arg Cys Leu Ala Pro Glu Thr Glu Thr
115 120 125Ser Ser Gln Glu Leu Thr Cys Met Tyr Gln Ser Tyr Gly Cys Glu Asp
130 135 140Met Tyr Pro Tyr Tyr Ser Glu Leu Arg His Glu Ala His Cys Asn Phe145 150 155 160Arg Pro Tyr Asn Cys Pro Tyr Ala Gly Ser Glu Cys Lys Leu Val Gly
165 170 175Asp Ile Pro Phe Leu Val Ala His Leu Arg Asp Asp His Lys Val Tyr
180 185 190Met His Asn Ser Cys Thr Phe Asp His Arg Tyr Val Lys Ser Asn Pro
195 200 205Leu Glu Val Glu Asn Ala Ile Trp Met Pro Thr Val Ile Asn Cys Phe
210 215 220Gly Gln Phe Phe Cys Leu His Phe Glu Ala Phe Leu Leu Asp Met Ala225 230 235 240Pro Val Tyr Ile Ala Phe Leu Ile Phe Met Gly Asp Asp Asn Glu Ala
245 250 255Lys Asn Phe Ser Tyr Cys Leu Glu Thr Gly Gly Asn Gly Arg Lys Leu
260 265 270Ile Trp His Gly Val Pro Arg Ser Ile Arg Asp Cys His Arg Lys Val
275 280 285His Asp Ser Ser Asp Gly Leu Ile Ile Gln Arg Asp Val Ala Leu Phe
290 295 300Phe Ser Gly Gly Asp Ile Asn Glu Leu Asn Leu Arg Leu Thr Gly His305 310 315 320Ile Leu Lys Glu Gln
325<210>202
<211>1474
<212>DNA
<213>辐射松(Pinus radiata)
<400>202gaattcggca cgagaaaacg tccatagctt ccttgccaac tgcaagcaat acagtacaag 60agccagacga tcgaatcctg tgaagtggtt ctgaagtgat gggaagcttg gaatctgaaa 120aaactgttac aggatatgca gctcgggact ccagtggcca cttgtcccct tacacttaca 180atctcagaaa gaaaggacct gaggatgtaa ttgtaaaggt catttactgc ggaatctgcc 240actctgattt agttcaaatg cgtaatgaaa tggacatgtc tcattaccca atggtccctg 300ggcatgaagt ggtggggatt gtaacagaga ttggcagcga ggtgaagaaa ttcaaagtgg 360gagagcatgt aggggttggt tgcattgttg ggtcctgtcg cagttgcggt aattgcaatc 420agagcatgga acaatactgc agcaagagga tttggaccta caatgatgtg aaccatgacg 480gcacacctac tcagggcgga tttgcaagca gtatggtggt tgatcagatg tttgtggttc 540gaatcccgga gaatcttcct ctggaacaag cggcccctct gttatgtgca ggggttacag 600ttttcagccc aatgaagcat ttcgccatga cagagcccgg gaagaaatgt gggattttgg 660gtttaggagg cgtggggcac atgggtgtca agattgccaa agcctttgga ctccacgtga 720cggttatcag ttcgtctgat aaaaagaaag aagaagccat ggaagtcctc ggcgccgatg 780cttatcttgt tagcaaggat actgaaaaga tgatggaagc agcagagagc ctagattaca 840taatggacac cattccagtt gctcatcctc tggaaccata tcttgccctt ctgaagacaa 900atggaaagct agtgatgctg ggcgttgttc cagagccgtt gcacttcgtg actcctctct 960taatacttgg gagaaggagc atagctggaa gtttcattgg cagcatggag gaaacacagg 1020aaactctaga tttctgtgca gagaagaagg tatcatcgat gattgaggtt gtgggcctgg 1080actacatcaa cacggccatg gaaaggttgg agaagaacga tgtccgttac agatttgtgg 1140tggatgttgc tagaagcaag ttggataatt agtctgcaat caatcaatca gatcaatgcc 1200tgcatgcaag atgaatagat ctggactagt agcttaacat gaaagggaaa ttaaattttt 1260atttaggaac tcgatactgg tttttgttac tttagtttag cttttgtgag gttgaaacaa 1320ttcagatgtt tttttaactt gtatatgtaa agatcaattt ctcgtgacag taaataataa 1380tccaatgtct tctgccaaat taatatatgt attcgtattt ttatatgaaa aaaaaaaaaa 1440aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa 1474
<210>203
<211>414
<212>DNA
<213>巨杉(Eucalyptus grandis)
<400>203cacgctcgac gaattcggta ccccgggttc gaaatcgata agcttggatc caaagcaaca 60cattgaactc tctctctctc tctctctctc tctctctctc tcccccaccc ccccttccca 120accccaccca catacagaca agtagatacg cgcacacaga agaagaaaag atgggggttt 180caatgcagtc aatcgcacta gcgacggttc tggccgtcct aacgacatgg gcgtggaggg 240cggtgaactg ggtgtggctg aggccgaaga ggctcgagag gcttctgaga cagcaaggtc 300tctccggcaa gtcctacacc ttcctggtcg gcgacctcaa ggagaacctg cggatgctca 360aggaagccaa gtccaagccc atcgccgtct ccgatgacat caagcctcgt ctct 414
<210>204
<211>755
<212>DNA
<213>辐射松(Pinus radiata)
<400>204ggcggccgtt cctaaggctc caatggcgat gggtgggagt gcagccaagg atgtggcaga 60ttccattgat tgggaggtta ggcctggagg aatgctggtt atgtggcaga ttccattgat 120tgggaggtta ggcctggagg aatgctggtt caaggtcaga gtcacctacg gctcttcctt 180gcatgaagtt tctgtcagta tgcagaccac atttggtgag ttgaaaaaac tacttgctcc 240tgagactgga ttagaaccac aagatcaaaa gcttatcttt agaggaaaag aaagggatgg 300caaggacttc ttagatttag caggtgtgaa agacaagtca aagatcgtgc ttatggaaga 360tccgatgagt cgagaaaaga agtacattga aatgaggaag aatgcaaaaa ttgagagggc 420gaccagagct attgctgacg tgagcctaga ggtggataaa cttgcagcac agttgtcttc 480cctggaagca ctgattgtta aaggcaaaag agtagctgaa aatgatttgg ttgacctcat 540tgaaatgctt atgagacaac tagtaaaatt agatagcatc ccagctgatg gagatgccaa 600attgcagaga agaatgcagg ttagaagggt gcaaaagtat gtcgagacat tggatgttct 660gaaggttccc aatgctacac agaactcatc atctcaacaa cctgtagtgg tgacaacaaa 720gtgggaaacc ttcgaaactt aattgccacc atgga 755
<210>205
<211>246
<212>PRT
<213>辐射松(Pinus radiata)
<400>205Ala Ala Val Pro Lys Ala Pro Met Ala Met Gly Gly Ser Ala Ala Lys1 5 10 15Asp Val Ala Asp Ser Ile Asp Trp Glu Val Arg Pro Gly Gly Met Leu
20 25 30Val Met Trp Gln Ile Pro Leu Ile Gly Arg Leu Gly Leu Glu Glu Cys
35 40 45Trp Phe Lys Val Arg Val Thr Tyr Gly Ser Ser Leu His Glu Val Ser
50 55 60Val Ser Met Gln Thr Thr Phe Gly Glu Leu Lys Lys Leu Leu Ala Pro65 70 75 80Glu Thr Gly Leu Glu Pro Gln Asp Gln Lys Leu Ile Phe Arg Gly Lys
85 90 95Glu Arg Asp Gly Lys Asp Phe Leu Asp Leu Ala Gly Val Lys Asp Lys
100 105 110Ser Lys Ile Val Leu Met Glu Asp Pro Met Ser Arg Glu Lys Lys Tyr
115 120 125Ile Glu Met Arg Lys Asn Ala Lys Ile Glu Arg Ala Thr Arg Ala Ile
130 135 140Ala Asp Val Ser Leu Glu Val Asp Lys Leu Ala Ala Gln Leu Ser Ser145 150 155 160Leu Glu Ala Leu Ile Val Lys Gly Lys Arg Val Ala Glu Asn Asp Leu
165 170 175Val Asp Leu Ile Glu Met Leu Met Arg Gln Leu Val Lys Leu Asp Ser
180 185 190Ile Pro Ala Asp Gly Asp Ala Lys Leu Gln Arg Arg Met Gln Val Arg
195 200 205Arg Val Gln Lys Tyr Val Glu Thr Leu Asp Val Leu Lys Val Pro Asn
210 215 220Ala Thr Gln Asn Ser Ser Ser Gln Gln Pro Val Val Val Thr Thr Lys225 230 235 240Trp Glu Thr Phe Glu Thr
245
<210>206
<211>347
<212>DNA
<213>辐射松(Pinus radiata)
<400>206tacccttgga gttgtcgatt cctacgtgtt caagagcaac gtagtgaagc tagacgtata 60agacgttgag catggttcga cttctagtag ctagacatac accagatgcg tcaagagtgc 120cgtcaacacg tccaacagat ctaccgttat caacctagta aaggaaagtt acgaaaagaa 180agtcctcaag atagaacgtg ccccagtcga cacgaacgtc aaacaaacgc ctacgttcag 240ttgtttcttt tgttccttaa gttcctagag ggaggtcttt ctctaaacgt ctaaaacaaa 300acacgttaga acatgaagtg aaccactatt acttgaagga tccaatc 347
Claims (22)
1.分离的多核苷酸,含有选自下组的多核苷酸序列:(1)SEQ IDNOS:1-45、90-140、192-195、200、204和206列举的序列;(2)SEQ ID NOS:1-45、90-140、192-195、200、204和206列举序列的互补体;(3)SEQ ID NOS:1-45、90-140、192-195、200、204和206列举序列的反向互补体;(4)SEQ ID NOS:1-45、90-140、192-195、200、204和206列举的序列的反向序列;(5)序列,包括具有与对比序列有至少50%同一性的多核苷酸序列,该对比序列选自SEQ IDNOS:1-7,10-18,20-31,35-40,43,44,90-94,98-107,109,110,112-118,120,121,123-140,192-195,200,204,和206,列举的多核苷酸序列,应用BLASTN程序通过2.04版本将该序列与对比序列进行匹配测定同一性百分比,该程序设定如下运行参数值:Unix运行命令:blastall-p blastn-dembldb-e 10-G0-E0-r1-v 30-b 30-i queryseq-o结果,确定该序列与对比序列在匹配部分相同核苷酸的数目,将相同核酸的数目除以对比序列核酸的总数,再乘以100以测定相似性百分比;(6)序列,包括与对比序列有至少75%同一性的多核苷酸序列,对比序列选自SEQ ID NOS:8、9、33、34、41、42、96、108和111列举的多核苷酸序列,按以上(5)中所述进行同一性百分比测定;(7)序列,包括与对比序列有至少90%同一性的多核苷酸序列,对比序列选自SEQID NOS:32、95、97、119和122列举的多核苷酸序列,按以上(5)中所述进行同一性百分比测定;(8)序列,包括在严格杂交条件下与包括上面(1)-(7)所述序列的多核苷酸杂交的核苷酸序列;(9)序列,包括上面(1)-(7)所述序列的200-链节的多核苷酸序列;(10)序列,包括上面(1)-(7)所述序列的100-链节的多核苷酸序列;和(11)序列,包括的多核苷酸序列与上面(1)-(4)中所述序列的差异仅在于1或多个保守替换。
2.含有权利要求1的至少一个多核苷酸的遗传构建体。
3.含有权利要求1的至少两个多核苷酸的遗传构建体。
4.含有权利要求2的至少一个遗传构建体的转基因林业植物细胞。
5.遗传构建体包括,5’-3’方向:
(a)基因启动子序列;
(b)包括至少下列一个多核苷酸的多核苷酸序列:(1)包括权利要求1中核苷酸序列的多核苷酸,该核苷酸序列编码PCD途径中活性多肽至少一个功能部分和(2)包括权利要求1中核苷酸序列的多核苷酸,该核苷酸序列含有编码在PCD途径中具有活性的多肽的多核苷酸非翻译区;和
(c)基因终止序列。
6.权利要求5的构建体,其中该多核苷酸是有义方向的。
7.权利要求5的构建体,其中该多核苷酸是反义方向的。
8.权利要求5的构建体,其中该基因启动子序列和基因终止序列在林业植物宿主中具有功能。
9.含有权利要求5的构建体的林业植物细胞。
10.权利要求9的林业植物细胞,其中该多核苷酸是有义方向的。
11.权利要求9的林业植物细胞,其中该多核苷酸是反义方向的。
12.含有权利要求9的林业植物细胞的林业植物,和果实和种子及其子代。
13.权利要求12的林业植物,其中该林业植物是木本植物。
14.权利要求13的林业植物,其中该植物选自由桉树和松树物种组成的组别。
15.调节林业植物中PCD途径的方法,包括稳定地将权利要求2的构建体掺入该林业植物的基因组中。
16.权利要求15的方法,其中掺入该林业植物基因组中的构建体提供在该林业植物的自然形式中不存在的PCD途径。
17.在林业植物中调节细胞死亡途径的方法,包括将权利要求5的构建体稳定掺入该林业植物的基因组中。
18.制备含有改变的细胞死亡途径的林业植物的方法,包括:
(a)应用权利要求2的构建体转化植物细胞以提供转基因植物细胞;和
(b)在益于繁殖和植物生长成熟的条件下培养该转基因植物细胞。
19.权利要求1的多核苷酸编码的分离的多肽。
20.权利要求19的在林业植物细胞死亡途径中具有活性的多肽。
21.含有多核苷酸表达氨基酸序列的分离多肽,该多核苷酸与SEQ ID NOS:1-45、90-140、192-195、200、204和206所示序列在严格杂交条件下杂交。
22.包括选自以下成分所组成之组别的氨基酸序列的分离多肽:(1)SEQ ID NOS:46-89、141-191、196-199、201和205所示序列;(2)包括与对比序列至少有50%同一性的氨基酸序列的序列,对比序列选自SEQ ID NOS: 46,48-50,55,58,60,61,80,81,87-89,141,142,144,156,157,160,161,173,174,177,179,180,182-191,198和205,列举的氨基酸序列;(3)包括与对比序列至少有75%同一性的氨基酸序列的序列,对比序列选自SEQ ID NOS:49,57,59,66,67,72,82-84,143,145,149,150,152-155,159,162-173,178,181,196,197,199,和201,所列举的氨基酸序列;(4)包括与对比序列至少有90%同一性的氨基酸序列的序列,对比序列选自SEQ ID NOS:51-54,56,62-65,68-71,73,74,79,146,147,151,158,和176,所列举的氨基酸序列;(5)包括与对比序列至少有98%同一性的氨基酸序列的序列,对比序列选自75、78、85、86和148所列举的氨基酸序列。
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US09/325,932 US6451604B1 (en) | 1999-06-04 | 1999-06-04 | Compositions affecting programmed cell death and their use in the modification of forestry plant development |
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CN113416747A (zh) * | 2020-03-03 | 2021-09-21 | 山东舜丰生物科技有限公司 | 一种创建温度敏感型雄性不育植物的方法 |
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US20020116734A1 (en) * | 2000-09-14 | 2002-08-22 | Dickman Martin B. | Plant derived bag homologues |
WO2004062366A2 (en) * | 2003-01-03 | 2004-07-29 | The Texas A & M University System | Stem-regulated, plant defense promoter and uses thereof in tissue-specific expression in monocots |
RU2375452C2 (ru) * | 2003-03-12 | 2009-12-10 | Басф Плант Сайенс Гмбх | Способ повышения стойкости к стрессовым факторам в растениях |
DE102006018583B4 (de) * | 2006-04-21 | 2017-05-11 | Alyoniq Ag | Abwehrstoff gegen die Bildung von Haustorien als Pflanzenschutzmittel gegen Pflanzenparasiten |
US7977535B2 (en) | 2006-07-12 | 2011-07-12 | Board Of Trustees Of Michigan State University | DNA encoding ring zinc-finger protein and the use of the DNA in vectors and bacteria and in plants |
EP2081954B1 (de) * | 2006-10-24 | 2012-06-13 | BASF Plant Science GmbH | Verfahren zur erhöhung der resistenz gegen biotrophe pilze in pflanzen |
EP2527455A3 (en) | 2008-11-03 | 2013-03-06 | Swetree Technologies AB | Vegetabile material, plants and a method of producing a plant having altered lignin properties |
US10106813B2 (en) | 2014-02-10 | 2018-10-23 | Board Of Trustees Of Michigan State University | Drought-tolerance in plants |
US20160017316A1 (en) * | 2014-07-18 | 2016-01-21 | Bionano Genomics, Inc. | Isolation of megabase-sized dna from plant and animal tissues |
EP3124493A1 (en) * | 2015-07-31 | 2017-02-01 | Albert-Ludwigs-Universität Freiburg | Overexpression of plant genes involved in programmed cell death for yield increase |
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CN113416747A (zh) * | 2020-03-03 | 2021-09-21 | 山东舜丰生物科技有限公司 | 一种创建温度敏感型雄性不育植物的方法 |
CN113416747B (zh) * | 2020-03-03 | 2023-09-12 | 山东舜丰生物科技有限公司 | 一种创建温度敏感型雄性不育植物的方法 |
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US6451604B1 (en) | 2002-09-17 |
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EP1187921A4 (en) | 2003-08-27 |
AU5258000A (en) | 2000-12-28 |
BR0011322A (pt) | 2002-03-05 |
MXPA01012398A (es) | 2003-09-10 |
JP2003501094A (ja) | 2003-01-14 |
WO2000075331A1 (en) | 2000-12-14 |
CA2375409A1 (en) | 2000-12-14 |
US20030082724A1 (en) | 2003-05-01 |
AR024172A1 (es) | 2002-09-04 |
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