CN1335053A - Method of bacteria-free germination and fast propagation of peltate yam - Google Patents
Method of bacteria-free germination and fast propagation of peltate yam Download PDFInfo
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- CN1335053A CN1335053A CN 01128802 CN01128802A CN1335053A CN 1335053 A CN1335053 A CN 1335053A CN 01128802 CN01128802 CN 01128802 CN 01128802 A CN01128802 A CN 01128802A CN 1335053 A CN1335053 A CN 1335053A
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Abstract
The present invention provides one kind of bacteria-free germination and fast propagation method of seed of dioscorea zingiberensis and belongs to the field of biological technology. The method of the present invention can shorten the breeding period of good variety of dioscorea zingiberensis and improve the quality of and raise the yield of bred good dioscorea zingiberensis variety.
Description
The present invention relates to biological technical field, relate to dioscorea zingiberensis wright seed asepsis sprouting and the method for breeding fast particularly.
In the prior art, dioscorea zingiberensis wright (Dioscorea zingiberensis) rhizome breeding commonly used, but need kind of amount big, the cost height.Though seminal propagation can solve kind of a source problem at short notice, but characteristic because of its dioecism (minority homophyletic), natural hybrization is frequent, add its bad border of diversified mountain area plantation nature, caused the high heterogeneous of hereditary basis, genetic diversity is very abundant, and the seedling of hybridizing for 1 generation just separates, and good strains of seeds is difficult to stablize like this.
At prior art above shortcomings part, the dioscorea zingiberensis wright seed of selecting and purchasing high-load saponin of the present invention, a kind of dioscorea zingiberensis wright (Dioscorea zingiberensis) seed asepsis sprouting and the method for breeding fast are provided, this method has shortened the seed selection time of dioscorea zingiberensis wright good species system, has improved the quality and quantity of fine-variety breeding.
In order to realize above-mentioned purpose of the present invention, the invention provides following scheme:
Dioscorea zingiberensis wright seed asepsis sprouting and the method for breeding fast, comprise screening of seed asepsis sprouting and breeding and quick propagation steps, take the dioscorea zingiberensis wright seed, go to soak with suds behind the seed wing, rinse well, soak into 75% alcohol, 0.1% mercuric chloride sterilization 8-10min, aseptic water washing, be inoculated in the 1/4MS+PH5.8 medium of no hormone, at intensity of illumination 1000LX, light application time 12h/d carries out seed asepsis sprouting under the condition that temperature is 25 ± 1 ℃, change on the medium MS+6-BA0.2mg/l+IAA0.1mg/l+NAA0.1mg/l+PH5.8 and breed fast, the clump bud of band root is divided into simple bud transfers strong sprout in the MS of no hormone medium, transplant.
Compared with prior art, beneficial effect of the present invention is:
1, selecting and purchasing dioscorea zingiberensis wright seed of the present invention, a seed is a line of breeding, sprout the back by number breeding of kind of subsystem at aseptic condition, when treating that each seed reaches some, each takes out several strains and transplants, carry out the screening of outdoor breeding, outdoor The selection result instructs indoor kind subsystem going or staying, therefore can obtain growth cycle weak point, high yield, good species subsystem disease-resistant, that saponin content is high and carry out expanding propagation.
2, the present invention's breeding, the synchronization of taking root, the seed selection time of dioscorea zingiberensis wright good species system has been shortened in indoor and outdoor fine-variety breeding synchronization greatly, improves the quality and quantity of fine-variety breeding, has saved soil and manpower.
3, the present invention organically combines sexual propagation and vegetative propagation in test tube, has fully shown the high benefit application of modern biotechnology in the dioscorea zingiberensis wright industrialization is produced.
Further specify essentiality content of the present invention with embodiments of the invention below, but content of the present invention is not limited thereto:
Embodiment 1:
1, materials and methods
Seed picks up from wild dioscorea zingiberensis wright, its saponin content 3%, select big, the full seed of grain, go to soak 20min with suds behind the seed wing, rinse well with running water again, on super bacterium platform, soak into 30s with 75% alcohol, 0.1% mercuric chloride sterilization, 8～10min behind the aseptic water washing several, is inoculated on the different medium, carry out sprouting test under different illumination conditions and different temperature, the result is as follows:
1.1 medium and hormone are to the influence of dioscorea zingiberensis wright seed asepsis sprouting
At intensity of illumination 1000LX, the result of the test under the condition of culture that 12h/d illumination and temperature are 25 ± 1 ℃ shows (medium PH5.8) (table 1), and seed germination is fast in the medium of no hormone, and the germination rate height adds to sprout behind the hormone being suppressed.Medium concentration becomes negative correlation with seed germination, the suitable seed germination of 1/4MS medium.Observe to find, the seed seedling of in no hormone culture-medium, sprouting, the tubercle about its epicotyl formation 0.01-0.05cm, leaf with medium concentration increase, color burn.Have the medium seed germination of hormone slow, increase with hormone concentration, the tubercle that epicotyl forms increases, petiole, cane overstriking, and leaf quantity also increases, and has occurred eye on the stem tuber.
Medium that table 1 is different and hormone are to the influence of dioscorea zingiberensis wright seed asepsis sprouting:
1.2 kernel maturing is to the influence of its axenic germination 1/4MS+PH5.8 medium with no hormone, at intensity of illumination 1000LX, light application time 12h/d carries out the culture experiment of kernel maturing under the condition that temperature is 25 ± 1 ℃.Result's (table 2) shows that the dioscorea zingiberensis wright seed does not have resting stage, and germination rate becomes positive correlation with kernel maturing, becomes negative correlation with the resting period.
| Medium | Sowing number (grain) | Sowing 30d | Sowing 60d | |||
| Germinative number (grain) | Germination rate (%) | Germinative number (grain) | Germination rate (%) | Stem tuber size (cm) | ||
| 1/4Ms ?1/2MS ?MS ?MS＋BA0.2＋NAA ?0.05 ?MS＋BA0.5＋NAA ?0.05 | ?100 ?100 ?100 ?100 · ?100 | ?56 ?56 ?13 ?6 ?2 | ?56 ?56 ?13 ?6 ?2 | ?96 ?88 ?72 ?53 ?37 | ?96 ?88 ?72 ?53 ?37 | ?0.01～0.05 ?0.01～0.05 ?0.01～0.05 ?0.05～0.1 ?0.1～0.2 |
Table 2 kernel maturing is to the influence (statistical number of sowing 60d) of its axenic germination
| Kernel maturing (color and luster) | The seeds time (room temperature is deposited) | Sowing quantity (grain) | Seed germination number (grain) | Germination rate (%) |
| Seed light green color seed yellow-white seed amber seed brown seed brown seed brown | ----1 year half a year | ?20 ?20 ?20 ?20 ?20 ?20 | ?17 ?19 ?19 ?19 ?18 ?17 | ?85 ?95 ?95 ?95 ?90 ?85 |
1.3 temperature is to the influence of dioscorea zingiberensis wright seed asepsis sprouting
At the medium of the 1/4MS+PH5.8 of no hormone, intensity of illumination 1000LX, the sprouting test under the condition of light application time 12h/d shows (table 3), rise with temperature, the dioscorea zingiberensis wright seed germination rate improves, and temperature seed inertia when being lower than 15 ℃, seed germination also is suppressed when being higher than 30 ℃.This is consistent with the seed germination result of the test of Zhang Youde professor in soil.
Table 3 temperature is to the influence (statistical number of sowing 60d) of dioscorea zingiberensis wright seed asepsis sprouting
| Cultivation temperature (℃) | Sowing quantity (grain) | Sprout quantity (grain) | Germination rate (%) |
| ?10±1 ?15±1 ?20±1 ?25±1 ?28±1 ?30±1 | ?20 ?20 ?20 ?20 ?20 ?20 | 0 1 11 19 19 14 | ?0 ?5 ?55 ?95 ?95 ?70 |
1.4: illumination is to the influence of dioscorea zingiberensis wright seed germination
With the medium of no hormone 1/4MS+PH5.8, in cultivation temperature: carry out the comparative test of different light time and intensity of illumination under 25 ± 1 ℃ the condition of culture.Result's (table 4) shows that illumination does not have influence to the dioscorea zingiberensis wright seed germination, is cultivating 60 days, and its percentage of seedgermination can both reach 95%.
Table 4 illumination is to the influence of dioscorea zingiberensis wright seed germination
| Illumination | Seeding quantity | Germination quantity | Germination rate |
| 1, dark 2,1000LX illumination 12h/d 3,2000LX illumination 12h/d | ?100 ?100 ?100 | ?95 ?95 ?95 | 95％ 95％ 95％ |
1.5: the aseptic fast breeding technique of dioscorea zingiberensis wright kind subsystem
Set up seed germination offspring's the system of nourishing and generating.On medium MS+6-BA0.2mg/l+IAA0.1mg/l+NAA0.1mg/l+PH5.8,30d can breed 5 times of clump buds, and 2～3 roots of clump bud band of 90% are arranged, and the clump bud of band root is divided into simple bud transfers strong sprout in the MS of no hormone medium, just can transplant transplanting survival rate 85% behind the 20d.The synchronization of breeding like this, take root, the seed selection time of dioscorea zingiberensis wright good species system has been shortened in indoor and outdoor fine-variety breeding synchronization greatly, improves the quality and quantity of fine-variety breeding, has saved soil and manpower.
Claims (1)
1, dioscorea zingiberensis wright seed asepsis sprouting and the method for breeding fast, comprise screening of seed asepsis sprouting and breeding and quick propagation steps, it is characterized in that adopting the dioscorea zingiberensis wright seed, go to soak with suds behind the seed wing, rinse well, soak into 75% alcohol, 0.1% mercuric chloride sterilization 8-10min, aseptic water washing, be inoculated in the 1/4MS+PH5.8 medium of no hormone, at intensity of illumination 1000LX, light application time 12h/d carries out seed asepsis sprouting under the condition that temperature is 25 ± 1 ℃, change on the medium MS+6-BA0.2mg/l+IAA0.1mg/l+NAA0.1mg/l+PH5.8 and breed fast, the clump bud of band root is divided into simple bud transfers strong sprout in the MS of no hormone medium, transplant.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011288027A CN1147220C (en) | 2001-08-29 | 2001-08-29 | Method of bacteria-free germination and fast propagation of peltate yam |
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| CNB011288027A CN1147220C (en) | 2001-08-29 | 2001-08-29 | Method of bacteria-free germination and fast propagation of peltate yam |
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| CN1335053A true CN1335053A (en) | 2002-02-13 |
| CN1147220C CN1147220C (en) | 2004-04-28 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1327761C (en) * | 2005-03-31 | 2007-07-25 | 东北师范大学 | Method of breeding in vitro of small tuber for dioscorea nipponica Makino |
| CN100358413C (en) * | 2005-04-22 | 2008-01-02 | 云南农业大学 | Cymbidium plant seed asepsis sprouting and plant cultivating method |
| CN103416183A (en) * | 2013-06-24 | 2013-12-04 | 重庆市药物种植研究所 | Artificial cultivation method of dioscorea zingiberensis |
| CN104067938A (en) * | 2014-06-24 | 2014-10-01 | 中国科学院亚热带农业生态研究所 | Tissue culture one-step seedling formation batch production method of medicinal peltate yam rhizome |
| CN104904601A (en) * | 2015-06-11 | 2015-09-16 | 中国科学院亚热带农业生态研究所 | Rooting method of sterile short shoot of medicinal peltate yam rhizome tissue culture seedling |
-
2001
- 2001-08-29 CN CNB011288027A patent/CN1147220C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1327761C (en) * | 2005-03-31 | 2007-07-25 | 东北师范大学 | Method of breeding in vitro of small tuber for dioscorea nipponica Makino |
| CN100358413C (en) * | 2005-04-22 | 2008-01-02 | 云南农业大学 | Cymbidium plant seed asepsis sprouting and plant cultivating method |
| CN103416183A (en) * | 2013-06-24 | 2013-12-04 | 重庆市药物种植研究所 | Artificial cultivation method of dioscorea zingiberensis |
| CN103416183B (en) * | 2013-06-24 | 2016-05-04 | 重庆市药物种植研究所 | Dioscorea zingiberensis wright artificial method for planting |
| CN104067938A (en) * | 2014-06-24 | 2014-10-01 | 中国科学院亚热带农业生态研究所 | Tissue culture one-step seedling formation batch production method of medicinal peltate yam rhizome |
| CN104904601A (en) * | 2015-06-11 | 2015-09-16 | 中国科学院亚热带农业生态研究所 | Rooting method of sterile short shoot of medicinal peltate yam rhizome tissue culture seedling |
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| Publication number | Publication date |
|---|---|
| CN1147220C (en) | 2004-04-28 |
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