CN1324956C - Biological disease prevention honeycomb matrix and its production method - Google Patents
Biological disease prevention honeycomb matrix and its production method Download PDFInfo
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- CN1324956C CN1324956C CNB2004100125130A CN200410012513A CN1324956C CN 1324956 C CN1324956 C CN 1324956C CN B2004100125130 A CNB2004100125130 A CN B2004100125130A CN 200410012513 A CN200410012513 A CN 200410012513A CN 1324956 C CN1324956 C CN 1324956C
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Abstract
The present invention relates to a biological honeycomb foundation which can prevent diseases. A composition which is composed of biological insecticide, biological miticide, beneficial microorganisms and nutrient substances is added in a base body of the honeycomb foundation. The base body of the honeycomb foundation comprises components of the proportion by weight: 20 to 30 % of yellow wax, 40 to 50 % of 75 DEG ceresin wax and 25 to 35 % of paraffin. The added composition of per kilogram of the base body comprises the components: 0.1 to 1 g of the biological insecticide, 0.1 to 10 g of the biological miticide, 0.2 to 20 ml of the beneficial microorganisms and 0 to 20 g of amino acid. The biological honeycomb foundation which can prevent diseases of the present invention has the advantages of simple and convenient production, low cost, safe use, no chemical pollution, etc., and the present invention can be widely applied to prevent honeycomb insects and different sizes of bee mites of Apis mellifera and Chinese bees and prevent and treat bee-crawl diseases, chalkbrood diseases and Euramerican foulbrood of the Apis mellifera and sac brood of the Chinese bees.
Description
Technical field
The invention belongs to septum and the production method thereof used in a kind of honeybee beehive, be specifically related to a kind of septum and production method thereof that adopts the comprehensive organism Prevention Technique to be used to prevent and treat the honeybee common disease.
Background technology
The honeybee damage by disease and insect is the first-class difficult problem that bee-keeping is produced, common bee colony disease mainly contains: the nest worm of apis mellifera (being called for short Apis mellifera down) and Chinese honeybee (honeybee in being called for short down), big chalcid fly mite, the crawl disease of bee of Apis mellifera, the sac brood of chalk disease and European and American foulbrook and middle honeybee etc., its etiology is insect or bacterium, fungi, microorganisms such as virus, the general employing of the prevention of honeybee disease and treatment fed, the method of sprinkling or stifling chemical agent, in the treatment disease, unavoidably more or less bee colony and bee product are polluted, and then to taking the human health generation harm of bee product, the new honeybee disease of necessary for this reason exploitation is prevented and treated method.Essential product during septum is produced as bee-keeping and since its with honeybee frequently closely contact probability become the first-selected ideal carrier that the honeybee disease is prevented and treated new method naturally.Yet general septum only has the effect that the honeyflow honeybee is nested, and does not have the honeybee disease to prevent and treat function; " a kind of biology yield increasing septum and method for making thereof " (Chinese patent ZL98118962.8) adopts and add female sex hormone and amino acid whose method in septum, when stimulating the queen bee farrowing, improving bee product output, can partly improve the immunity of bee colony, but the bee colony disease not had the effect of symptomatic treatment yet; And the septum that adds chemical agent adopts antibiotic methods such as forbidden drugs and sulfamethoxyplridazine, tetracycline, terramycin such as interpolation sulfenone, galecron as " anti-beemite prevent the foulbrood beehive base " (Chinese patent ZL91105952); though honeybee disease is had certain prevention and result of treatment; yet because it adopts forbidden drugs and antibiotic; pollute even more serious to bee colony and bee product generation; obviously be unfavorable for taking the healthy of bee product crowd, also be unfavorable for environmental protection simultaneously.
Summary of the invention
The objective of the invention is to develop a kind of employing biological synthesis Prevention Technique, effectively prevent and treat the honeybee common disease and do not produce the septum and the production method thereof of chemical contamination.
Biological disease-preventing septum of the present invention is the composition that adds biologic products such as biological insecticides, biological miticide, beneficial microbe and nutriment in the septum matrix, and the percentage by weight of septum matrix consists of:
20～30%75 ° of ceresine 40～50% of yellow wax, paraffin 25～35%.
The composition that adds consists of:
Biological insecticides 0.1～10g/kg matrix,
Biological miticide 0.1～10g/kg matrix,
Beneficial microbe 0.2～20ml/kg matrix,
Amino acid 0～20g/kg matrix.
Described yellow wax also can be beeswax.But described paraffin commercial grade.
Described biological insecticides are bacillus thuringiensis (Bacillus Thuringien) or its mutation such as pine caterpillars bacillus, bacillus thuringensis, bacillus thruingensis etc., its toxicity evaluation is 8000～24000 international units (IU)/mg, adopt wetting powder, if adopt suspending agent (seeing agricultural industry criteria NY293/95) to be converted into the required volume of corresponding wettable powder agent concentration according to the principle that toxicity evaluation equates.
Described biological miticide is macro tetrolide or nikkomycin, and macro tetrolide is 5wt%～90wt% emulsifiable concentrate, and nikkomycin is 1wt%～50wt% wetting powder.
Described beneficial microbe (Effective Microorganisms) is the suspension that the microorganism of the summations of about more than the 80 kinds of microorganisms of 5 sections such as photosynthetic bacteria, lactic acid bacteria, Bifidobacterium, saccharomycete and actinomycetes or its branch is formed, and its viable count is 10
9～3 * 10
9Individual/ml.
Described amino acid is methionine or lysine, is white solid, and the former is a crystal fine grain, and the latter is a powder.
Production method step of the present invention is as follows:
1, the percentage by weight of pressing the septum matrix is formed 20～30%, 75 ° of ceresine 40～50% of yellow wax, and paraffin 25～35% ℃ dissolves yellow wax, ceresine, heating paraffin to 70～80;
2, the amino acid with 0～20g/kg joins in the wax liquid that dissolves, and mixes (without this step, directly entering next step when amount of amino acid is 0);
3, roll into the sheet wax of the thick 1～3mm of being after the wax liquid cooling but with mixing, roll into two-sided hexagon honeycomb decorative pattern with the flower machine again, simultaneously biological insecticides, biological miticide and beneficial microbe are pressed biological insecticides 0.1～10g/kg matrix, biological miticide 0.1～10g/kg matrix, after beneficial microbe 0.2～20ml/kg matrix adds water and stirs, join in the groove of colored machine below and evenly be pressed into septum, be cut into rectangular artificial honeycomb at last owing to carry.
Production method of the present invention also can be carried out as follows:
1, with above-mentioned step 1;
2, roll into the sheet wax of the thick 1～3mm of being after the wax liquid cooling but with mixing;
3, press biological insecticides 0.1～10g/kg matrix, biological miticide 0.1～10g/kg matrix, beneficial microbe 0.2～20ml/kg matrix, amino acid 0～20g/kg matrix proportion of composing (when amount of amino acid is 0, not adding amino acid), with biological insecticides, biological miticide, beneficial microbe suspension and amino acid solid adding additives mix to thick, evenly be coated on the surface of sheet wax, on it coated with the sheet wax of another uncoated, after rolling into a new sheet wax, crosses a ray machine flower machine, or directly excessively colored machine, roll into two-sided hexagon honeycomb decorative pattern, be cut into rectangular artificial honeycomb at last.
Described binding agent can be one or both in honey, epoxy resin, the white glue with vinyl.
Described thick mixing material slime flux thick outside the sheet wax edge not when being meant even apply.
The present invention is compared with prior art:
Biological disease-preventing septum of the present invention have produce easy, with low cost, safe in utilization, do not have advantage such as chemical contamination, can be widely used in the prevention and the treatment of sac brood of crawl disease of bee, chalk disease and European and American foulbrook and the middle honeybee of the nest worm of Apis mellifera and middle honeybee, big chalcid fly mite, Apis mellifera.On probation showing: the control efficiency to the nest worm promptly reached 100% after 5 days, and after lasting till 30 days, to crawl disease of bee, chalk disease and the European and American foulbrook of big chalcid fly mite, Apis mellifera and in the control efficiency of sac brood of honeybee after 15 days all greater than after 90%, 30 day all greater than 99%.
Embodiment
Embodiment 1: 250g yellow wax, 450g ceresine and 300g heating paraffin to 70 ℃ are dissolved and mix, 10g lysine is added wherein and mix, wax liquid is poured in the opening tub of flat, rectangular, water-cooled is also rolled into the sheet wax of thick about 1mm through ray machine, when the embossing of flower machine is that bacillus thuringiensis suspension 1ml, 2.5% nikkomycin wetting powder 20g and the viable count of 16000IU/l is 2 * 10 with toxicity evaluation
9Being mixed with in the groove that joins colored machine below after beneficial microorganism suspension 2ml adding suitable quantity of water stirs of individual/ml all is pressed into septum, is cut into rectangular artificial honeycomb at last.
Embodiment 2: with 250g yellow wax, 500g ceresine and ℃ fusion of 250g heating paraffin to 80 and mix, a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices, water-cooled, ray machine roll into the sheet wax of thick about 1.5mm, with toxicity evaluation is the pine caterpillars bacillus wetting powder 0.1g of 8000IU/mg, and 10% macro tetrolide missible oil 1g, viable count are 10
9The Bifidobacterium suspension 0.5ml of individual/ml and 2g lysine and proper honey and white glue with vinyl mix, evenly be coated on the surface of a sheet wax, on it coated with the sheet wax of the above-mentioned mixed liquor of another uncoated, roll into a new sheet wax after a ray machine, be cut into rectangular artificial honeycomb after the embossing of flower machine.
Embodiment 3: 300g yellow wax, 400g ceresine and 300g heating paraffin to 75 ℃ are dissolved and mix, the 20g methionine is added wherein and mix, wax liquid is poured in the opening tub of flat, rectangular, water-cooled is also rolled into the sheet wax of thick about 2mm through ray machine, when the embossing of flower machine is that bacillus thuringensis wetting powder 1g, 50% nikkomycin wetting powder 2g and the viable count of 8000IU/l is 10 with toxicity evaluation
9The photosynthetic bacteria suspension 20ml of individual/ml adds in the groove that joins colored machine below after suitable quantity of water stirs and all is pressed into septum, is cut into rectangular artificial honeycomb at last.
Embodiment 4: with 200g yellow wax, 500g ceresine and ℃ fusion of 300g heating paraffin to 75 and mix, a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices, water-cooled, ray machine roll into the sheet wax of thick about 2.5mm, with toxicity evaluation is the bacillus thruingensis wetting powder 10g of 16000IU/mg, and 20% nikkomycin wetting powder 25g and viable count are 2 * 10
9The lactic acid bacteria suspension 10ml of individual/ml and proper honey and epoxy resin mix, and evenly are coated on the surface of a sheet wax, coated with the sheet wax of the above-mentioned mixed liquor of another uncoated, are cut into rectangular artificial honeycomb after the embossing of the direct machine of flower excessively on it.
Embodiment 5: 250g, 400g ceresine and 350g heating paraffin to 80 ℃ are dissolved and mix, wax liquid is poured in the opening tub of flat, rectangular, water-cooled is also rolled into the sheet wax of thick about 3mm through ray machine, when the embossing of flower machine is that bacillus thuringiensis wetting powder 5g, 50% macro tetrolide missible oil 5g and the viable count of 24000IU/l is 3 * 10 with toxicity evaluation
9The actinomycetes suspension 5ml of individual/ml adds suitable quantity of water and stirs and all be pressed into septum in the groove that joins colored machine below, is cut into rectangular artificial honeycomb at last.
Embodiment 6: with 200g yellow wax, 450g ceresine and ℃ fusion of 350g heating paraffin to 70 and mix, a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices, water-cooled, ray machine roll into the sheet wax of thick about 2mm, with toxicity evaluation is the bacillus thruingensis suspension 5ml of 8000IU/mg, and 90% macro tetrolide missible oil 11g, viable count are 3 * 10
9Saccharomycete suspension 0.2ml, 5g methionine and the proper honey of individual/ml mix, evenly be coated on the surface of a sheet wax, on it coated with the sheet wax of the above-mentioned mixed liquor of another uncoated, roll into a new sheet wax after a ray machine, be cut into rectangular artificial honeycomb after the embossing of flower machine.
6 kinds of septums of the foregoing description 1～6 are used for the nest worm of Apis mellifera and honeybee, the control of big chalcid fly mite, at first respectively 2 every kind new septum displacements are infected 2 old honeycombs of the standard group of nest worm or big chalcid fly mite, repeated after three days, 10 beehive is all until the 13rd day old honeycombs are all replaced.Inspect the parasitic number of nest worm before the control on the old honeycomb that displaces for the first time during control nest worm, and enter the 5th day, the 15th day, the 30th day of this biological disease-preventing septum in displacement and inspect in the beehive the parasitic number of nest worm after the control on all honeycombs, calculate control efficiency.When preventing and treating big chalcid fly mite by honeybee mite mean parasitized number in 100 capping lairs of method statistic of 13～15 pages of " China's bee-keeping " 5 phases of nineteen ninety " the exhausted test report of mite of compound " and before the control on 100 honeybee bodies, again in checked in the 5th day, the 15th day, the 30th day in 100 capping lairs and 100 honeybee bodies on control after honeybee mite mean parasitized number, and calculate control efficiency respectively, the two on average is average control efficiency.The computing formula of its control efficiency is as follows, the results are shown in Table 1.
Table 1 biological disease-preventing septum is used for the nest worm of Apis mellifera and honeybee, the control efficiency of big chalcid fly mite
| The nest worm | Big chalcid fly mite | |||||||
| Borer population/spleen | Preventive effect % | Mite number/honeybee | Preventive effect % | Mite number/nest | Preventive effect % | Average preventive effect % | ||
| Embodiment 1 septum | Before the control | 8.5 | -- | 3.21 | -- | 6.12 | -- | -- |
| After 5 days | 0 | 100 | 1.69 | 47.35 | 3.82 | 37.58 | 42.47 | |
| After 15 days | 0 | 100 | 0.11 | 96.57 | 0.30 | 95.10 | 95.84 | |
| After 30 days | 0 | 100 | 0 | 100 | 0 | 100 | 100 | |
| Embodiment 2 septums | Before the control | 6.0 | -- | 10.64 | -- | 15.23 | -- | -- |
| After 5 days | 0 | 100 | 4.19 | 60.62 | 9.41 | 38.21 | 49.42 | |
| After 15 days | 0 | 100 | 1.05 | 90.13 | 1.47 | 90.35 | 90.24 | |
| After 30 days | 0 | 100 | 0 | 100 | 0 | 100 | 100 | |
| Embodiment 3 septums | Before the control | 7.5 | -- | 4.96 | -- | 6.73 | -- | -- |
| After 5 days | 0 | 100 | 0.79 | 84.07 | 2.41 | 64.19 | 74.13 | |
| After 15 days | 0 | 100 | 0.03 | 99.40 | 0.24 | 96.43 | 97.92 | |
| After 30 days | 0 | 100 | 0 | 100 | 0 | 100 | 100 | |
| Embodiment 4 septums | Before the control | 11.0 | -- | 7.49 | -- | 9.28 | -- | -- |
| After 5 days | 0 | 100 | 3.64 | 51.40 | 4.91 | 47.09 | 49.25 | |
| After 15 days | 0 | 100 | 0.51 | 93.19 | 0.87 | 90.63 | 91.91 | |
| After 30 days | 0 | 100 | 0 | 100 | 0 | 100 | 100 | |
| Embodiment 5 septums | Before the control | 9.5 | -- | 2.54 | -- | 4.42 | -- | -- |
| After 5 days | 0 | 100 | 1.02 | 59.84 | 2.15 | 51.36 | 55.60 | |
| After 15 days | 0 | 100 | 0.24 | 90.55 | 0.43 | 90.27 | 90.41 | |
| After 30 days | 0 | 100 | 0 | 100 | 0 | 100 | 100 | |
| Embodiment 6 septums | Before the control | 4.5 | -- | 8.73 | -- | 10.96 | -- | -- |
| After 5 days | 0 | 100 | 3.14 | 64.03 | 4.87 | 55.57 | 59.80 | |
| After 15 days | 0 | 100 | 0.22 | 97.48 | 1.08 | 90.15 | 93.81 | |
| After 30 days | 0 | 100 | 0 | 100 | 0 | 100 | 100 | |
6 kinds of septums of the foregoing description 1～6 are used for the sac brood of crawl disease of bee, chalk disease and European and American foulbrook and the honeybee of Apis mellifera, and method of replacing is the same.The crawl disease of bee of Apis mellifera shows as disease honeybee belly and expands, and is slow in action, and the little volume of wing is sagging, can not circle in the air, and can only creep around beehive, and midgut bleaches to expand and swells, and ring grain disappears, the long-pending full green watery stools of hindgut; The chalk disease show as that dead larva corpse becomes dry, hardening, form the chalk shape, adularescent is like the mycelia of cotton fiber shape or capsule spore spherical in shape is arranged; The dead larva that shows as of European and American foulbrook is pale asphyxia at first, is pitchy at last, has the acid smell flavor, and is inviscid, can not pull into filament; In honeybee sac brood to show as ill larva dead in the capping lair mostly, dead larva is yellowish-brown, without putrefaction is inviscid, no stink.At first dead (disease) honeybee number of every hive bee group before the displacement on the same day is added up, to not circling in the air and the quantity of the honeybee that creeps around the crawl disease of bee statistics beehive, haul out beehive nest dead larva quantity outdoors for chalk disease and European and American foulbrook statistics by worker bee, sac brood is chosen out the quantity that 100 capping lairs are added up dead larva immediately, and enter the 5th day, the 15th day, the 30th day of this biological disease-preventing septum in displacement dead (disease) honeybee number of every hive bee group is added up, statistical method is the same, and calculates control efficiency.The computing formula of its control efficiency is as follows, the results are shown in Table 2.
Table 2 biological disease-preventing septum is used for the control efficiency of crawl disease of bee, chalk disease, foulbrood, sac brood
| The Apis mellifera crawl disease of bee | Apis mellifera chalk disease | The Apis mellifera foulbrood | Chinese bee sacbrood | ||||||
| Sick honeybee number | Preventive effect % | Dead honeybee number | Preventive effect % | Dead honeybee number | Preventive effect % | Dead honeybee number/nest | Preventive effect % | ||
| Embodiment 1 septum | Before the control | 323 | - | 86 | - | 59 | - | 0.11 | - |
| After 5 days | 169 | 47.68 | 51 | 40.70 | 31 | 47.46 | 0.03 | 72.73 | |
| After 15 days | 29 | 91.02 | 5 | 94.19 | 5 | 91.53 | 0.01 | 90.91 | |
| After 30 days | 1 | 99. 69 | 0 | 100 | 0 | 100 | 0 | 100 | |
| Embodiment 2 septums | Before the control | 243 | - | 103 | - | 96 | - | 0.08 | - |
| After 5 days | 101 | 58.44 | 61 | 40.78 | 45 | 53.13 | 0.02 | 75.00 | |
| After 15 days | 22 | 90.95 | 10 | 90.29 | 8 | 91.67 | 0 | 100 | |
| After 30 days | 2 | 99.18 | 1 | 99.03 | 0 | 100 | 0 | 100 | |
| Embodiment 3 septums | Before the control | 468 | - | 64 | - | 46 | - | 0.33 | - |
| After 5 days | 204 | 56.41 | 23 | 64.06 | 13 | 71.74 | 0.12 | 63.64 | |
| After 15 days | 39 | 91.67 | 2 | 96.88 | 3 | 93.48 | 0.02 | 93.94 | |
| After 30 days | 3 | 99.36 | 0 | 100 | 0 | 100 | 0 | 100 | |
| Embodiment 4 septums | Before the control | 165 | - | 79 | - | 78 | - | 0.25 | - |
| After 5 days | 84 | 49.09 | 43 | 45.57 | 38 | 51.28 | 0.09 | 64.00 | |
| After 15 days | 10 | 93.94 | 7 | 91.14 | 7 | 91.03 | 0.02 | 92.00 | |
| After 30 days | 0 | 100 | 0 | 100 | 0 | 100 | 0 | 100 | |
| Embodiment 5 septums | Before the control | 209 | - | 121 | - | 63 | - | 0.018 | - |
| After 5 days | 112 | 46.41 | 71 | 41.32 | 25 | 60.32 | 0.07 | 61.11 | |
| After 15 days | 18 | 91.39 | 11 | 90.91 | 5 | 92.06 | 0.01 | 91.44 | |
| After 30 days | 1 | 99.52 | 1 | 99.17 | 0 | 100 | 0 | 100 | |
| Embodiment 6 septums | Before the control | 361 | - | 58 | - | 31 | - | 0.28 | - |
| After 5 days | 192 | 46.81 | 31 | 46.55 | 12 | 61.29 | 0.10 | 64.29 | |
| After 15 days | 34 | 90.58 | 4 | 93.10 | 2 | 93.55 | 0.02 | 92.86 | |
| After 30 days | 3 | 99.17 | 0 | 100 | 0 | 100 | 0 | 100 | |
Claims (9)
1, a kind of biological disease-preventing septum is characterized in that it being the composition that adds biological insecticides, biological miticide, beneficial microbe and composition of nutritive substance in the septum matrix, and the percentage by weight of septum matrix consists of:
Yellow wax 20～30% 75 ° ceresine 40～50%, paraffin 25～35%
The composition that adds consists of:
Biological insecticides 0.1～10g/kg matrix,
Biological miticide 0.1～10g/kg matrix,
Beneficial microbe 0.2～20ml/kg matrix,
Amino acid 0～20g/kg matrix.
2, a kind of biological disease-preventing septum as claimed in claim 1 is characterized in that described biological insecticides are bacillus thuringiensis or its pine caterpillars bacillus, bacillus thuringensis, bacillus thruingensis mutation, and its toxicity evaluation is 8000～24000 international units (IU)/mg.
3, a kind of biological disease-preventing septum as claimed in claim 1 or 2 is characterized in that described biological insecticides are wetting powder or suspending agent.
4, a kind of biological disease-preventing septum as claimed in claim 1 is characterized in that described biological miticide is macro tetrolide or nikkomycin, and macro tetrolide is 5wt%～90wt% emulsifiable concentrate, and nikkomycin is 1wt%～50wt% wetting powder.
5, a kind of biological disease-preventing septum as claimed in claim 1, it is characterized in that described beneficial microbe is the suspension that the microorganism of the summation of photosynthetic bacteria, lactic acid bacteria, Bifidobacterium, saccharomycete and the multiple microorganism of actinomycetes or its branch is formed, its viable count is 10
9～3 * 10
9Individual/ml.
6, a kind of biological disease-preventing septum as claimed in claim 1 is characterized in that described amino acid is methionine or lysine.
7,, it is characterized in that comprising that step is as follows as the production method of each a kind of biological disease-preventing septum of claim 1-6:
(1) percentage by weight of pressing the septum matrix is formed 20～30%, 75 ° of ceresine 40～50% of yellow wax, and paraffin 25～35% ℃ dissolves yellow wax, ceresine, heating paraffin to 70～80;
(2) amino acid with 0～20g/kg joins in the wax liquid that dissolves, and mixes;
(3) roll into the sheet wax of the thick 1～3mm of being after the wax liquid cooling but with mixing, roll into two-sided hexagon honeycomb decorative pattern with the flower machine again, simultaneously biological insecticides, biological miticide and beneficial microbe are pressed biological insecticides 0.1～10g/kg matrix, biological miticide 0.1～10g/kg matrix, after beneficial microbe 0.2～20ml/kg matrix adds water and stirs, join in the groove of colored machine below and evenly be pressed into septum, be cut into rectangular artificial honeycomb at last owing to carry.
8,, it is characterized in that comprising that step is as follows as the production method of each a kind of biological disease-preventing septum of claim 1-6:
(1) percentage by weight of pressing the septum matrix is formed 20～30%, 75 ° of ceresine 40～50% of yellow wax, and paraffin 25～35% ℃ dissolves yellow wax, ceresine, heating paraffin to 70～80;
(2) roll into the sheet wax of the thick 1～3mm of being after the wax liquid cooling but with mixing;
(3) press biological insecticides 0.1～10g/kg matrix, biological miticide 0.1～10g/kg matrix, beneficial microbe 0.2～20ml/kg matrix, amino acid 0～20g/kg matrix proportion of composing, with biological insecticides, biological miticide, beneficial microbe suspension and amino acid solid adding additives mix to thick, evenly be coated on the surface of sheet wax, on it coated with the sheet wax of another uncoated, after rolling into a new sheet wax, crosses a ray machine flower machine, or directly excessively colored machine, roll into two-sided hexagon honeycomb decorative pattern, be cut into rectangular artificial honeycomb at last.
9, the production method of a kind of biological disease-preventing septum as claimed in claim 8 is characterized in that described binding agent is one or both in honey, epoxy resin, the white glue with vinyl.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100125130A CN1324956C (en) | 2004-09-06 | 2004-09-06 | Biological disease prevention honeycomb matrix and its production method |
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|---|---|---|---|
| CNB2004100125130A CN1324956C (en) | 2004-09-06 | 2004-09-06 | Biological disease prevention honeycomb matrix and its production method |
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| CN1596625A CN1596625A (en) | 2005-03-23 |
| CN1324956C true CN1324956C (en) | 2007-07-11 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101810201A (en) * | 2010-04-16 | 2010-08-25 | 中国农业科学院蜜蜂研究所 | Biological preparation for preventing and treating nest insects |
| CN102247405B (en) * | 2011-08-19 | 2013-01-02 | 杨荣良 | Method for preparing preparation for treating and preventing Chinese sacbrood |
| CN103238631B (en) * | 2013-05-23 | 2014-06-04 | 中国农业科学院蜜蜂研究所 | Environment-friendly insecticide for killing wax-moth larvae and eggs thereof |
| CN105454316A (en) * | 2015-12-20 | 2016-04-06 | 欧必煜 | Formula of Chinese bee Chinese herbal medicine comb foundation and making method |
| CN107466908A (en) * | 2017-08-07 | 2017-12-15 | 福建农林大学 | A kind of septum that can reduce agricultural chemicals and pesticide residues |
| CN111316934B (en) * | 2018-12-17 | 2021-10-26 | 重庆师范大学 | Bee birth and fertility analyzer and method |
| CN109874702B (en) * | 2019-04-12 | 2021-09-10 | 云南农业大学 | Method for manufacturing epoxy resin honeycomb model by using temperature demoulding |
-
2004
- 2004-09-06 CN CNB2004100125130A patent/CN1324956C/en not_active Expired - Fee Related
Non-Patent Citations (1)
| Title |
|---|
| 多功能巢础造脾治螨除虫生产实验报告 王孟林，蜜蜂杂志，第01期 2000 * |
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