CN1317540A - Powdered stevia rebaudiana and enriched liquid and mfg. process thereof - Google Patents
Powdered stevia rebaudiana and enriched liquid and mfg. process thereof Download PDFInfo
- Publication number
- CN1317540A CN1317540A CN 00106501 CN00106501A CN1317540A CN 1317540 A CN1317540 A CN 1317540A CN 00106501 CN00106501 CN 00106501 CN 00106501 A CN00106501 A CN 00106501A CN 1317540 A CN1317540 A CN 1317540A
- Authority
- CN
- China
- Prior art keywords
- sweet stevia
- powder
- concentrated solution
- sweet
- stevia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
A powder and concentrated liquid of stevia rebaudinum is disclosed, which does not contain synthetic substance and can be used to clean soil, improved growth of animal and plane and prmote health of people. Said powder is a mixture of the stevia rebaudnium leaf powder and the stevia rebaudnium stem powder. Said concentrated liquid is prepared from said powder through extracting in hot water, concentrating, fermenting and ageing.
Description
The present invention relates to sweet Stevia powder and manufacture method thereof and sweet Stevia concentrated solution and manufacture method thereof, this product can be used as the soil improvement material that cleaning soil is used, the perhaps animal and plant growth auxiliary agent that various animals and plants are worked, perhaps be added on the sweetening agent in food, the pharmaceuticals, also can in fertilizer, feed, food, pharmaceuticals, use in addition.
Handling aspect the contaminated soil, often not to need integrated mill, be exactly cost is too high, be difficult in the time of most realize, placed without any measure ground.In plant growing,, use a lot of deleterious agricultural chemicals or residual environmentally harmful chemical fertilizer for the mouthfeel that improves food or output, pre-preventing disease and pest as agriculture central task.To the VITAMIN of the cheapness of having used chemosynthesis as the domestic animal of animal industry central task or use the mixed feed of agricultural chemicals, the cheap cultivation of chemical fertilizer, used microbiotic during disease.In food, added the sweet Stevia sweeting agent that extracts from sweet Stevia in the promptly so-called heath food as simple sweetening agent.In addition, hormone preparation or microbiotic etc. in pharmaceuticals, have been used.
In the processing of contaminated soil, need not to build integrated mill, also need not too high cost, only the concentrated solution that stirs sweet Stevia by spraying in soil just can reduce the toxicity of contaminated soil, can realize labor-saving to greatest extent processing.In plant growing as agriculture central task, the sweet Stevia powder is spread in the soil or the diluent of sweet Stevia concentrated solution is scattered in the irrigation water of soil or on the blade face, can make plant materials become healthy, be difficult for being encroached on by disease and pest.Thus, the agricultural chemicals that use pollutes environment can be reduced, and residual farm chemical ingredients or chemical fertilizer, cleaning soil can be decomposed.And plant is as agricultural-food, and taste and harvest yield can improve.To domestic animal, sweet Stevia powder or concentrated solution separately or be mixed in the feed and feed, can play improvement and under severe condition, raise the disease that stress and cause that causes as the animal industry central task.Improve meat or newborn matter.Need not at food, be to add sweet Stevia powder or sweet Stevia concentrated solution in the so-called heath food as simple sweetening agent, only need to use separately or in food, add, just can make blood glucose value, blood pressure, immunologic function recover normal by diet etc., also have the pharmacological action that improves stomach and intestine or hepatic diseases.
The raw material of sweet Stevia powder and sweet Stevia concentrated solution is the composite family perennial plant sweet Stevia of using as the sweeting agent raw material that originates in Paraguay (Stevia rebaudiana Bertoni), and its security is determined.When using, can consider the side effect by the chemical process synthetic drug and use restriction etc. according to above-mentioned using method.
The object of the present invention is to provide sweet Stevia powder and manufacture method thereof and sweet Stevia concentrated solution and manufacture method thereof, wherein do not conform to synthetic, is raw material with the sweet Stevia only.
As the sweet Stevia of raw material of the present invention, can be plant arbitrarily such as offspring or cuttage seeding or tree seedling etc., wish to cultivate annual harvesting 1 time by the organic farming of no agricultural chemicals.
With above-mentioned sweet Stevia drying, the leaf part is separated with stem portion.Leaf after the separation and stem separated pulverizing mix stevioside leaf powder and the sweet Stevia stem powder pulverized to about 20 orders.The ratio of stevioside leaf powder is adjusted into 10%～40%, is the sweet Stevia powder.At this moment, should not mix fully into the composition beyond any sweet Stevia.
Above-mentioned sweet Stevia powder with hot water is extracted, from sweet Stevia, extracted liquid, this liquid reconcentration, adjustment concentration, fermentation, slaking.
Below specify the present invention.In this specification sheets, if do not add specified otherwise, then described ratio all is as the criterion with weight.
(preparation of sweet Stevia powder)
The offspring of sweet Stevia or cuttage seeding or tree seedling etc. plant arbitrarily, to cultivate by the organic farming of no agricultural chemicals, 1 gained person is as the raw material sweet Stevia in annual harvesting.Stevioside leaf is taken with hand before harvesting, behind the excision leaf, at the bottom-up 10cm of sweet Stevia stem place the sweet Stevia stem is cut off.
Stevioside leaf that above-mentioned hand is plucked and the sweet Stevia stem cut off are in plastic greenhouse or use the drying machine drying.Make moisture content drop to 5%～10%.
The sweet Stevia cured leaf of above-mentioned gained and sweet Stevia scapus separated pulverizing are to about 20 orders.Stevioside leaf powder after pulverizing and the sweet Stevia stem powder mixed with 2: 8 is stirred.The mixed powder of gained is the sweet Stevia powder thus.
(preparation of sweet Stevia concentrated solution)
Above-mentioned sweet Stevia powder with hot water is extracted and is handled.The blending ratio of sweet Stevia powder and water is sweet Stevia powder 1kg, 10～15 liters in water.Extraction time is different because of the composition of sweet Stevia powder, temperature, humidity etc., preferred 30 minutes～2 hours.This abstraction process carries out 1～3 time.Make extracting solution and sweet Stevia powder separation with adding pressure type, spiral or rotary type water extracter, do not contained the pure extracting solution of sweet Stevia powder.
The extracting solution that abstraction process is obtained concentrates in enrichment process.Concentrate modes such as adopting evaporating kettle or decompression heating.Preferably reach and end to concentrate when 8 degree～25 are spent in the pol of concentrated solution.
With the slaking of fermenting in the sealed vessel with weather resistance of packing into of the concentrated solution of last operation gained.Has reliever in the sealed vessel.The fermentation slaking does not add vibration and left standstill 90～360 days at 10 degree～25 degree Celsius.
If promote fermentation, can certainly join the yeast of cultivating in advance in the concentrated solution, carry out above-mentioned fermentation curing step then.
The fermentation slaking liquid of gained is the sweet Stevia concentrated solution thus.This sweet Stevia concentrated solution can directly use with stoste, perhaps also can water etc. dilution be solution.
Main component is the effective constituent of sweet Stevia in sweet Stevia powder of the present invention and the sweet Stevia concentrated solution.Its main effect is as follows.
Environment: reduce the toxicity of objectionable impurities, improve the soil.
Plant: promote growth, improve pol or fragrance, improve harvest yield or quality, prevention and elimination of disease and pests improves the persistence after gathering.
Animal: promote growth, preventing disease improves a poor appetite, and improves and oestruses and conceptual quotient, and improvement stress.
Human: as to improve the symptom of viscera disease, improve pressure value and blood glucose value, improve immunity function, improve dermatosis etc.
Below enumerate embodiment the present invention is specifically described, but the present invention is not limit by embodiment.
Embodiment
Early October is gathered in the crops sweet Stevia, and stevioside leaf is plucked with hand getting, at the bottom-up 10cm of sweet Stevia stem place the sweet Stevia stem is cut off then.Utilize Exposure to Sunlight and drying machine drying.Make moisture drop to 5%.Above-mentioned sweet Stevia cured leaf and sweet Stevia scapus are crushed to 20 orders with pulverizer respectively.Stevioside leaf powder after pulverizing and sweet Stevia stem powder stirred with 2: 8 mixed make the sweet Stevia powder.
Add above-mentioned sweet Stevia powder 1kg in the pot that the aluminum volume is 20 liters, add 10 liters in water, heating.Began heated and stirred 1 hour from boiling, and make it acutely not seethe with excitement.Mixture is installed in the cotton bag, separate sweet Stevia powder and extracting solution with the rotary type water extracter.Isolated extracting solution is transferred in the pot of 20 liters of volumes of another mouthful.Sweet Stevia powder after the dehydration adds 8 liters in water again, moves in the pot and heats.Began heated and stirred 1 hour from boiling, and make it acutely not seethe with excitement.Mixture is installed in the cotton bag, separate sweet Stevia powder and extracting solution with the rotary type water extracter.The extracting solution of 7 liters of isolated extracting solutions and the 1st time is mixed, and heating concentrates 5 hours.Obtain 1 liter of sweet Stevia concentrated solution thus.With the fermentation slaking 180 days of packing in the sealed vessel with weather resistance of the concentrated solution of last operation gained, obtain the sweet Stevia concentrated solution thus.
The sweet Stevia powder of the foregoing description gained and the experimental example of sweet Stevia concentrated solution are as follows.
Grape is used
| Kind | The red spirit of grape (セ キ レ イ) |
| Using method | Soil broadcasts sowing 1 blade face of 500 times of liquid of sweet Stevia concentrated solution and broadcasts sowing 700 times of liquid of sweet Stevia concentrated solution 3 times |
| The result | Pol is checked sweet Stevia concentrated solution application zone 18.2 check plots 16.6 |
Grape is used
| Kind | Grape リ-ス リ Application ゲ |
| Using method | Soil broadcasts sowing 3 blade faces of 500 times of liquid of sweet Stevia concentrated solution and broadcasts sowing 800 times of liquid of sweet Stevia concentrated solution 5 times |
| The result | The sweet Stevia concentrated solution uses district's contrast pol 18.6 18.3 acid 0.60% 0.58 pH 3.90 3.81 |
Greenhouse melon (Ha ウ ス メ ロ Application) is used
| Kind | The greenhouse cultivation muskmelon |
| Using method | Bear fruit back the 10th day is removed and is broadcast sweet Stevia powder 1.5kg for per 10 ares |
| The result | Pol is checked sweet Stevia concentrated solution application zone 15.6 check plots 14.0 |
Greenhouse melon is used
| Kind | The greenhouse cultivation muskmelon |
| Using method | Bear fruit the back the 10th day per 2.5 ares broadcast sowing sweet Stevia powder 0.5kg |
| The result | Pol is checked sweet Stevia concentrated solution application zone 16.0 check plots 14.0 |
Greenhouse papaya (Ha ウ ス パ パ イ ヤ) is used
| Kind | Greenhouse papaya (Ha ウ ス パ パ イ ヤ) |
| Using method | When bearing fruit per 10 ares broadcast sowing sweet Stevia powder 2kg really long to ball when big or small soil broadcast sowing 500 times of liquid of sweet Stevia concentrated solution 1 time |
| The result | The sweet Stevia concentrated solution uses 56 52 pols 20 16 of district's contrast harvest yield |
Beef cattle (black wool and kind are female) is raised with the sweet Stevia powder
Ratio in 0.2% in the feed of the bad ox of the developmental condition that 4 are imported is simultaneously added, and selects 5 of normal oxen and measures body weight after about 1 month in contrast, estimates developmental condition.
| No | Ox name | Body weight before adding | Body weight after 33 days | The body weight increment | Every day the body weight increment | |
| The interpolation group | ????1 ????2 ????3 ????4 | ????3080 ????3096 ????3081 ????3087 | ?514????kg ?544 ?552 ?535 | ?541????kg ?572 ?581 ?560 | ?27?kg ?28 ?29 ?25 | ?0.818 ?0.848 ?0.878 ?0.757 |
| Mean value | ?27.25 | ?0.825 | ||||
| Control group | ????5 ????6 ????7 ????8 ????9 | ????3067 ????3061 ????2896 ????2908 ????2971 | ?530 ?563 ?620 ?529 ?626 | ?541 ?589 ?634 ?612 ?660 | ?11 ?26 ?14 ?20 ?34 | ?0.333 ?0.787 ?0.424 ?0.606 ?1.030 |
| Mean value | ?21.00 | ?0.636 | ||||
The interpolation group was only taken food about 7kg before adding, and when just having raised with the sweet Stevia powder, because the foreign odor of sweet Stevia, feed slightly reduces, and appearance to be added such as had just become after a few days, and stink also has been accustomed to, and appetite reaches more than the 10kg, not residue.It is as shown in the table for body weight increment after 1 month, the few especially reason of body weight increment every day of control group can think the corral environmental degradation cause.The interpolation group shows the normal development more than 0.825, and in appearance, fetal hair disappears, and hair also shows gloss.Result after delivering for sale, das Beinfleisch has medium above on the upper side meat.
The test of sweet Stevia concentrated solution Dui dioxin effect
(1) purpose
Have the information of dioxin capacity of decomposition according to the sweet Stevia concentrated solution, confirm whether the sweet Stevia concentrated solution has the ability of decomposing actual burning ash Zhong dioxin.
(2) experimental technique
The known burning ash of dioxin concentration is adopted in experiment.
Operation is carried out by the following method.
(2-1) in transparent 200mL Erlenmeyer flask, add the grey 1g of burning, add sweet Stevia concentrated solution 100mL, stirred 10 days with stirrer.
(2-2) stir after 10 days, leach solid substance with glass fiber filter paper.
(2-3) dioxins materials in analysis solid substance (leaching thing) and the liquid (filtrate) confirms whether decompose.Replace the sweet Stevia concentrated solution as blank with ion exchanged water, carry out above-mentioned same operation.Analysis is according to " offal treatment Zhong dioxin standard test handbook is carried out.
(3) result
| Dioxin concentration (PCDD s) | Unit | Diphenylene-oxide concentration (PCDF s) | Unit | Toxicity concentration TEQ of equal value | Unit | |
| The sweet Stevia blank | ????3100 | ?ng/g | ????710 | ?Ng/g | ????44 | ?ng-TEQ/g |
| Sweet Stevia 10 days | ????1700 | ?ng/g | ????440 | ?Ng/g | ????26 | ?ng-TEQ/g |
Acute toxicity test (LD
50)
(1) uses animal
Mouse ddy is male every group 10 3 groups of 5 all ages
(2) test method
Administration: select healthy mice, fasting is used after 18 hours and is irritated stomach pin oral administration experimental liquid (stoste) by force.
Dosage: 10 is 1 group, and each group is pressed the amount administration of every 1mL/20g, 0.75mL/20g, 0.5mL/20g respectively.
The raising condition: under the fixed temperature and humidity condition, ad lib water and feed between 0～7 day after the administration.
(3) observe process
Maximum dosage-feeding 1mL/20g group, all discovery in about 30 minutes has autonomic movement low after the administration, symptoms such as respiration inhibition, full recovery in back 30 minutes.0.75mL/20g administration group in addition, after the administration accidental autonomic movement low, recover about 20 minutes after the administration.
(4) test-results
Measure body weight every day according to above-mentioned raising condition, observe general symptom, body weight normally increases therebetween, does not observe special phenomenon in 7 days.By above result, the LD50 of this sweet Stevia concentrated solution is more than the 50ml/kg, and it is extremely low to infer acute toxicity.
Claims (14)
1. sweet Stevia powder is with stevioside leaf powder and sweet Stevia stem powder mixes and obtain.
2. according to the sweet Stevia powder of claim 1, wherein the blending ratio of stevioside leaf powder is 10～40 overall weight % of powder.
3. sweet Stevia concentrated solution is that the sweet Stevia powder with hot water of claim 1 is extracted, concentrated, the fermentation of gained concentrated solution, slaking and concentrated solution.
4. according to the sweet Stevia concentrated solution of claim 4, the time of the slaking of wherein fermenting is 90～360 days.
5. the sweet Stevia manufacturing method of power is characterized in that the leaf of stevioside plant is separated with stem, and is respectively dry, the sweet Stevia cured leaf of gained and sweet Stevia scapus pulverized be to mix stevioside leaf powder and sweet Stevia stem powder and stir.
6. according to the sweet Stevia manufacturing method of power of claim 5, the moisture content of wherein dried sweet Stevia cured leaf and sweet Stevia scapus is 5%～10%.
7. according to the sweet Stevia manufacturing method of power of claim 5, wherein mix the stevioside leaf powder and the sweet Stevia stem powder that stir and be crushed to about 20 orders.
8. according to the sweet Stevia manufacturing method of power of claim 5, wherein the blending ratio of stevioside leaf powder is 10～40 overall weight % of powder.
9. the manufacture method of sweet Stevia concentrated solution is characterized in that just the sweet Stevia powder with hot water of claim 5 gained is extracted, concentrated, the fermentation of gained concentrated solution, slaking, thereby acquisition sweet Stevia concentrated solution.
10. according to the manufacture method of the sweet Stevia concentrated solution of claim 9, wherein the extraction time of hot water is 30 minutes to 2 hours.
11. according to the manufacture method of the sweet Stevia concentrated solution of claim 9, above-mentioned hot water extraction is carried out 1～3 time.
12. according to the manufacture method of the sweet Stevia concentrated solution of claim 9, the fermentation slaking, the fermentation curing time is 90～360 days.
13. according to the manufacture method of the sweet Stevia concentrated solution of claim 9, the temperature of fermentation slaking is 10 degree～25 degree Celsius.
14. according to the manufacture method of the sweet Stevia concentrated solution of claim 9, do not add vibration during the fermentation slaking, leave standstill.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00106501 CN1230493C (en) | 2000-04-07 | 2000-04-07 | Powdered stevia rebaudiana and enriched liquid and mfg. process thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00106501 CN1230493C (en) | 2000-04-07 | 2000-04-07 | Powdered stevia rebaudiana and enriched liquid and mfg. process thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1317540A true CN1317540A (en) | 2001-10-17 |
| CN1230493C CN1230493C (en) | 2005-12-07 |
Family
ID=4578345
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00106501 Expired - Fee Related CN1230493C (en) | 2000-04-07 | 2000-04-07 | Powdered stevia rebaudiana and enriched liquid and mfg. process thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1230493C (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101062077B (en) * | 2007-06-18 | 2011-04-06 | 石任兵 | Method for preparing stevia whole stevioside and stevia whole flavone at the same time |
| CN102001885A (en) * | 2010-10-28 | 2011-04-06 | 浙江大学 | Biological compound leaf fertilizer and preparation method thereof |
| CN101062078B (en) * | 2007-06-18 | 2011-04-06 | 石任兵 | Extract of stevia whole stevioside and stevia whole flavone and the preparing method thereof |
| CN102050678A (en) * | 2010-11-25 | 2011-05-11 | 海城市三星生态农业有限公司 | Stevia functional fertilizer and preparation method thereof |
| CN102199060A (en) * | 2011-04-06 | 2011-09-28 | 云南千州生物有机肥有限公司 | Production method of functional organic fertilizer used for tobacco |
| CN101674841B (en) * | 2007-04-06 | 2013-03-20 | 株式会社夏洛美 | Anti-histamine substance and method for production thereof |
| CN103141810A (en) * | 2013-03-27 | 2013-06-12 | 湖南农业大学 | Method for processing lactic acid fermentation type stevia rebaudian concentrated juice |
| CN110638852A (en) * | 2019-09-16 | 2020-01-03 | 上海维丘康敬生物科技有限公司 | Pure natural plant antioxidant derived from natural stevia rebaudiana and preparation method thereof |
| CN110981562A (en) * | 2019-09-16 | 2020-04-10 | 上海维丘康敬生物科技有限公司 | Use of stevia preparations in agriculture |
-
2000
- 2000-04-07 CN CN 00106501 patent/CN1230493C/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101674841B (en) * | 2007-04-06 | 2013-03-20 | 株式会社夏洛美 | Anti-histamine substance and method for production thereof |
| CN101062077B (en) * | 2007-06-18 | 2011-04-06 | 石任兵 | Method for preparing stevia whole stevioside and stevia whole flavone at the same time |
| CN101062078B (en) * | 2007-06-18 | 2011-04-06 | 石任兵 | Extract of stevia whole stevioside and stevia whole flavone and the preparing method thereof |
| CN102001885A (en) * | 2010-10-28 | 2011-04-06 | 浙江大学 | Biological compound leaf fertilizer and preparation method thereof |
| CN102001885B (en) * | 2010-10-28 | 2013-07-03 | 浙江大学 | Biological compound leaf fertilizer and preparation method thereof |
| CN102050678A (en) * | 2010-11-25 | 2011-05-11 | 海城市三星生态农业有限公司 | Stevia functional fertilizer and preparation method thereof |
| CN102199060A (en) * | 2011-04-06 | 2011-09-28 | 云南千州生物有机肥有限公司 | Production method of functional organic fertilizer used for tobacco |
| CN103141810A (en) * | 2013-03-27 | 2013-06-12 | 湖南农业大学 | Method for processing lactic acid fermentation type stevia rebaudian concentrated juice |
| CN110638852A (en) * | 2019-09-16 | 2020-01-03 | 上海维丘康敬生物科技有限公司 | Pure natural plant antioxidant derived from natural stevia rebaudiana and preparation method thereof |
| CN110981562A (en) * | 2019-09-16 | 2020-04-10 | 上海维丘康敬生物科技有限公司 | Use of stevia preparations in agriculture |
| CN110638852B (en) * | 2019-09-16 | 2022-05-17 | 上海维丘康敬生物科技有限公司 | Pure natural plant antioxidant derived from natural stevia rebaudiana and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1230493C (en) | 2005-12-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1618293A (en) | Non-pollution agricultural chemicals, and prepn. method therefor | |
| KR102222811B1 (en) | Additive composition for fertilizer and animal feed, and manufacturing mehtod thereof | |
| CN106912697A (en) | A kind of deodorant pig feed and preparation method thereof | |
| KR20190062995A (en) | Feed composition comprising soybean curd cake for Protaetia brevitarsis seulensis larva | |
| CN1230493C (en) | Powdered stevia rebaudiana and enriched liquid and mfg. process thereof | |
| CN101861953A (en) | Feed Chinese medical herb composition, feed additive, feed and preparation method | |
| CN110590444A (en) | Special bio-organic fertilizer for hot pepper and preparation method and application thereof | |
| KR102134543B1 (en) | Chicken feed composition | |
| CN104402617A (en) | Pleurotus eryngii cultivation material with use of garlic straw and pleurotus eryngii production method | |
| KR101168632B1 (en) | A feed composition | |
| KR100936015B1 (en) | Feed additives for livestock and feed including the same | |
| Makkar et al. | Effect of wood ash on tannin content of oak (Quercus incana) leaves | |
| CN104326821B (en) | A kind of banana stalks leaf fertilizer and manufacture method | |
| KR101042432B1 (en) | Manufacturing method of non-toxic mineral sulfur removed harmful substances and environment friendly non-antibiotic livestock breeding method using thereof | |
| CN1552234A (en) | Feed additive for improving animal disease-resistant ability | |
| KR20210010422A (en) | Feed additives for the sterilization, deodorization and promotion of decomposting of decomposted organic fertilizers and method of manufacture the same | |
| CN106900650A (en) | A kind of cultural method of selenium enriched chicken | |
| CN103283965B (en) | Plant flavor changer and method for preparing animal feed by using same | |
| CN103387429A (en) | Organic fertilizer and production method thereof | |
| Dasuki et al. | Preliminary investigation of nutritional quality of Senna obtusifolia for potential use in fish feed. | |
| KR101284727B1 (en) | Fermented bee feed using enzyme and method of producing the same | |
| CN112335786A (en) | Marigold stem leaf feed with acid aroma smell and preparation method thereof | |
| Bai et al. | Utilization of water hyacinth biomass for animal feed | |
| KR102627671B1 (en) | Composition of high concentration saponin fertilizer including ellow lacquer extract, The fertilizer using the composition, And the cultivation grow using the fertilizer | |
| Chin et al. | Supplementing Tithonia diversifolia with Guinea grass or tree foliages: effects on feed intake and live weight gain of growing goats |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |