CN1317497A - Process for preparing chitosan - Google Patents
Process for preparing chitosan Download PDFInfo
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- CN1317497A CN1317497A CN 01106664 CN01106664A CN1317497A CN 1317497 A CN1317497 A CN 1317497A CN 01106664 CN01106664 CN 01106664 CN 01106664 A CN01106664 A CN 01106664A CN 1317497 A CN1317497 A CN 1317497A
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Abstract
A process for preparing chitosan from the waste (mycelia0 generated in production of citric acid includes cleaning mycelia which may be blue mold, Aspergillus niger, or rhizopus, reaction with acid, reaction with alkali, oxidization to decolour, preparing chitin, deacetylation and obtaining finished chitosan. Its advantages include cheap raw material, simple process, and high quality.
Description
The present invention relates to technical process, particularly the preparation method of chitosan.
Chitosan is called the poly-acetamido glucose that takes off again.It extensively is present in the cell walls of the shells of ocean arthropods such as shrimp, crab etc. and fungi microbe such as fungies such as Ascomycetes, Basidiomycetes, algae and deuteromycetes.Chitosan has a wide range of applications in fields such as chemical industry, medicine industry, foodstuffs industry and agricultural, in recent years the report that has many chitosans to use in textile industry again.
At present, the technology of producing chitosan mainly can be divided into two classes from the raw material aspect.The first kind is that the shell with crustaceans such as shrimp, crabs is that main raw material is produced, because the supply of this class raw material is subjected to the influence in season to be subjected to the influence of region again in addition, therefore also be subjected to seasonal effect and cost higher with the chitosan of this class raw material production, the quality of its product also changes with the variation in the places of origin of raw materials on the other hand.The second class production technique is that raw material is produced with aseptic screwworm, because the raising of aseptic fly requirement is very high, therefore the chitosan cost of producing with these class methods is very high.
The objective of the invention is: provide that a kind of raw material cheaply is easy to get, the preparation method of the simple chitosan of production process.
For achieving the above object, solution of the present invention is: it comprise the steps: clean mycelium → with acid-respons → with alkali reaction → oxidative decoloration → chitin finished product → deacetylation → chitosan finished product; Be specially:
A, mycelium is put into acidic solution, be heated to 50～100 ℃, keep after 30～60 minutes filter to do.
B, the solid substance after filter among the step a done join and are heated to 50～100 ℃ and kept 10～60 minutes in the basic solution, and filter is done then.
C, the solid substance after filter among the step b done kept 10～60 minutes after joining and being heated to 50～100 ℃ in the oxidizing agent solution, and filter is done then.
D, the solid substance of gained among the step c joined in 0.1%～10% the potassium permanganate solution and soaked 10～120 minutes, the decolouring back adds a certain amount of oxalic acid solution and keeps 10～120 down then minute in 30～100 ℃, is washed to the filter of neutral back then and does.
E, in steps d, add 1～10 times of its weight in the solid substance of gained basic solution in 50～130 ℃ of reactions 0.5～30 hour down, filter then, wash to neutrality dry finished product again with water.
Mycelium is the Penicillium notatum of penicillin fermentation or the black-koji mould or the lactic acid fermented rhizopus of citric acid fermentation among the step a.
Owing to when fermentation production of citric acid, can produce a large amount of mycelium, contain 20% the chitin of having an appointment in these mycelium, contain protein and other carbohydrate in addition.The present invention destroys mycelial cell walls with the acid-alkali synthetic method, removes wherein protein and other carbohydrate then, obtains purified chitin, then chitin is carried out the deacetylation reaction again, obtains high-quality chitosan at last.
The processing method of the production chitosan that the present invention proposes, the waste material (mycelium) that is produced during with the production citric acid is that raw material is produced chitosan.This processing method has that raw material cheaply is easy to get, production process is simple and characteristics such as equipment used is less demanding, and these characteristics make that the chitosan that comes out with this explained hereafter is with low cost, and warp is compared with the commodity chitosan of import, and its quality can match in excellence or beauty with it.
Below embodiment of the present invention are described in further detail:
Get exsiccant mycelium 50 grams, put into 150 milliliter 1% HCl (or sulfuric acid) solution, boil 30 minutes after washing thalline to neutral, filter is done then.
The filter dry of above-mentioned gained is put into 150 milliliter 15% NaOH (or KOH) solution, be heated to behind the 80C and kept 30 minutes.And then wash with water to neutrality, filter is done.
Hydrogen peroxide (the H that in above-mentioned filter dry, adds 100 grams 10%
2O
2) solution (or chlorine bleach liquor), boiled again 35 minutes.The washing solid substance is to neutrality, and filter is done.
The solid substance of above-mentioned gained is joined 0.5% potassium permanganate (KMnO
4) soaked 1 hour in the solution, the decolouring back adds an amount of oxalic acid solution, keeps 30 minutes down in 70 ℃, be washed to the filter of neutral back do chitin.
Get 10 gram chitins, add 60 milliliter 50% NaOH (or KOH) solution, reacted 2 hours down in 110 ℃.Be washed to neutrality then, filter is done after 70 ℃ of vacuum-dryings get the chitosan finished product.
Claims (5)
1, a kind of preparation method of chitosan is characterized in that: comprise the steps:
A, mycelium is put into acidic solution, is heated to 50～100 ℃, keep after 30～60 minutes filter to do:
B, the solid substance after filter among the step a done join and are heated to 50～100 ℃ and kept 10～60 minutes in the basic solution, and filter is done then:
C, the solid substance after filter among the step b done kept 10～60 minutes after joining and being heated to 50～100 ℃ in the oxidizing agent solution, and filter is done then:
D, the solid substance of gained among the step c joined in 0.1%～10% the potassium permanganate solution and soaked 10～120 minutes, the decolouring back adds a certain amount of oxalic acid solution and keeps 10～120 down then minute in 30～100 ℃, is washed to the filter of neutral back then and does;
E, in steps d, add 1～10 times of its weight in the solid substance of gained basic solution in 50～130 ℃ of reactions 0.5～30 hour down, filter then, wash to neutrality dry finished product again with water:
Mycelium is the Penicillium notatum of penicillin fermentation or the black-koji mould or the lactic acid fermented rhizopus of citric acid fermentation among the step a.
2, preparation method as claimed in claim 1 is characterized in that: acidic solution is 0.1%～36% hydrochloric acid or 0.1%～98% sulfuric acid.
3, preparation method as claimed in claim 1 is characterized in that: the basic solution among the step b is 5%～50% NaOH or KOH.
4, preparation method as claimed in claim 1 is characterized in that: the basic solution among the step e is 10%～70% NaOH or KOH.
5, preparation method as claimed in claim 1 is characterized in that: the oxidizing agent solution among the step c is 1%～30% hydrogen peroxide or chlorine bleach liquor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01106664 CN1317497A (en) | 2001-04-27 | 2001-04-27 | Process for preparing chitosan |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01106664 CN1317497A (en) | 2001-04-27 | 2001-04-27 | Process for preparing chitosan |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1317497A true CN1317497A (en) | 2001-10-17 |
Family
ID=4655648
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01106664 Pending CN1317497A (en) | 2001-04-27 | 2001-04-27 | Process for preparing chitosan |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1317497A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100396703C (en) * | 2006-06-20 | 2008-06-25 | 青岛科技大学 | Preparation method of chitin |
| CN101974106A (en) * | 2010-11-18 | 2011-02-16 | 天津泰康生物制药有限公司 | Method for extracting chitin by utilizing citric-acid fermentation waste residue |
| CN101735337B (en) * | 2009-12-31 | 2012-05-30 | 中国科学院微生物研究所 | Method for preparing chitin and chitosan |
| CN103848927A (en) * | 2012-12-05 | 2014-06-11 | 浙江省农业科学院 | Method for preparing Beta-chitin by utilizing needle mushroom residues after extraction of polysaccharide |
-
2001
- 2001-04-27 CN CN 01106664 patent/CN1317497A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100396703C (en) * | 2006-06-20 | 2008-06-25 | 青岛科技大学 | Preparation method of chitin |
| CN101735337B (en) * | 2009-12-31 | 2012-05-30 | 中国科学院微生物研究所 | Method for preparing chitin and chitosan |
| CN101974106A (en) * | 2010-11-18 | 2011-02-16 | 天津泰康生物制药有限公司 | Method for extracting chitin by utilizing citric-acid fermentation waste residue |
| CN101974106B (en) * | 2010-11-18 | 2011-11-23 | 天津泰康生物制药有限公司 | Method for extracting chitin by utilizing citric-acid fermentation waste residue |
| CN103848927A (en) * | 2012-12-05 | 2014-06-11 | 浙江省农业科学院 | Method for preparing Beta-chitin by utilizing needle mushroom residues after extraction of polysaccharide |
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