CN100384992C - Method for improving stability of alkaline pectase in cotton spinning and refining process - Google Patents
Method for improving stability of alkaline pectase in cotton spinning and refining process Download PDFInfo
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- CN100384992C CN100384992C CNB2005100400293A CN200510040029A CN100384992C CN 100384992 C CN100384992 C CN 100384992C CN B2005100400293 A CNB2005100400293 A CN B2005100400293A CN 200510040029 A CN200510040029 A CN 200510040029A CN 100384992 C CN100384992 C CN 100384992C
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- pectase
- alkaline pectase
- refining
- cotton
- alkali
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- Chemical Or Physical Treatment Of Fibers (AREA)
- Detergent Compositions (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The present invention relates to a method for improving the stability of alkali pectase in cotton manufacturing refining technology, particularly to the technical field of the application technology of the alkali pectase in the cotton manufacturing refining technology. The present invention uses bacillus (Bacillus sp.)WSHB04-02 as original strains, and the alkali pectase is prepared by submerged fermentation. The application property of the alkali pectase is conspicuously enchanced by adding ammonium acetate and magnesium chloride of stabilizing agents, and the alkali pectase is suitable for refining cotton spinning fabrics at higher temperature. The present invention can be used for refining the alkali pectase, replace the traditional soda boiling refining technology, reduce the dosage of enzyme preparation, reduce cost, improve biological environment, and improve the product quality of the fabrics.
Description
Technical field
The present invention relates to the applied technical field of alkaline pectase in cotton spinning and refining process for a kind of method that improves alkaline pectase stability in cotton spinning and refining process.
Background technology
Crude fabric after the destarch, its water-absorbent and whiteness are all unsatisfactory.Tradition is concise after the concentrated base kiering, needs to consume a large amount of clear water and carries out rinsing, produces large amount of sewage, and is totally unfavorable to environment.As it is concise to adopt biological enzyme that fabric is carried out, and to remove non-cellulosic impurity, can reduce the influence to environment greatly.But alkaline pectase decompose pectin matter, and impurity such as lipid that will adhere to and protein are together removed from the cotton fibre surface.Carry out concisely with enzyme, can make and reduce to minimum the destruction of cotton fibre.Therefore, the good characteristic of alkaline pectase makes it more and more to be subjected to people's attention.
Summary of the invention
Stability when the objective of the invention is to improve alkaline pectase and at high temperature using is to adapt to the needs of cotton spinning and refining.Use the alternative traditional alkaline scouring technology of the present invention, reduce enzyme preparation amount, improve environment, improve the fabric product quality.
Technical scheme of the present invention: adopt genus bacillus (Bacillus sp.) WSHB04-02 as starting strain, this bacterial strain is open in another patent application (application number 200410065807.X) of the application unit, deposit number is CCTCC NO:M204082, alkaline pectase through the liquid submerged fermentation preparation, after Plate Filtration and ultrafiltration, obtain the liquid enzyme formulation of enzyme 200～300u/mL alive, add stablizer ammonium acetate 4～6% and MgCl in the quality percentage therein
2H
2O 1.5～2%.The refining process condition is: the zymin consumption that has added stablizer is 4～8mL/L, 55～65 ℃ of Applicable temperature scopes, pH scope 9.0～10.0,1～1.5 hour treatment time.
Beneficial effect of the present invention: improved the stability when alkaline pectase is at high temperature used greatly.It is concise that the alternative traditional alkaline scouring technology of described zymin is used for cotton-spinning fabric, reduces enzyme preparation amount to reduce production costs; Improve environment, reduced salts contg in the discharge water and COD etc.; Improve product quality, improved the flexibility and the intensity of cotton fabric, have good dyeing behavior simultaneously.
Embodiment 1
Adopt genus bacillus (Bacillus sp.) WSHB04-02 as starting strain, alkaline pectase through the liquid submerged fermentation preparation, after Plate Filtration and ultrafiltration, obtain the liquid enzyme formulation of enzyme 200～300u/mL alive, add stablizer ammonium acetate 4～6% and MgCl in the quality percentage therein
2H
2O 1.5～2%, make to use the high modified alkaline pectase preparation of stability.
Embodiment 2
With cotton fabric boiling water treating 10min, respectively with original enzyme liquid and the enzyme liquid concise (processing condition are enzyme dosage 4mL/L, bath raio 1: 30,57 ℃ of temperature, pH9.2, treatment time 75min) that has added stablizer, use the clear water rinsing more again.The latter's pectin clearance can reach 68.7%, satisfies concise requirement, and wettability can satisfy the requirement of bleaching and dyeing process; Pectin clearance in the former cotton has only 5.1%, can not satisfy concise requirement, influences the carrying out of subsequent technique.
Claims (2)
- One kind improve alkaline pectase in cotton spinning and refining process stability method, it is characterized in that adopting genus bacillus (Bacillus sp.) WSHB04-02, preserving number CCTCC NO:M204082 is as starting strain, alkaline pectase through the liquid submerged fermentation preparation, after Plate Filtration and ultrafiltration, obtain the liquid enzyme formulation of enzyme 200～300 μ alive/mL, add stablizer ammonium acetate 4%～6% and MgCl in the quality percentage therein 2H 2O 1.5%～2% is to improve the application stability of this alkaline pectase in the fabric refining process.
- 2. method using method of adding the alkaline pectase behind the stablizer according to claim 1, it is characterized in that being used for the refining processing of cotton-spinning fabric, processing condition are: the zymin consumption 4～8mL/L behind the interpolation stablizer, 55～65 ℃ of Applicable temperature scopes, pH scope 9.0～10.0,1～1.5 hour treatment time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100400293A CN100384992C (en) | 2005-05-12 | 2005-05-12 | Method for improving stability of alkaline pectase in cotton spinning and refining process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100400293A CN100384992C (en) | 2005-05-12 | 2005-05-12 | Method for improving stability of alkaline pectase in cotton spinning and refining process |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1699563A CN1699563A (en) | 2005-11-23 |
| CN100384992C true CN100384992C (en) | 2008-04-30 |
Family
ID=35475810
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100400293A Expired - Fee Related CN100384992C (en) | 2005-05-12 | 2005-05-12 | Method for improving stability of alkaline pectase in cotton spinning and refining process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100384992C (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101311272B (en) * | 2007-05-24 | 2011-06-22 | 中国科学院上海生命科学研究院 | Process for preparing acetone, butanol and ethanol by tapioca fermentation |
| CN101157900B (en) * | 2007-09-04 | 2011-07-27 | 江南大学 | Alkaline pectic enzyme producing engineering strain and its construction and method for producing alkaline pectic enzyme with the same |
| CN105524672A (en) * | 2016-01-28 | 2016-04-27 | 赛景波 | Production method of environment-friendly enzyme solution for coal |
| CN107740280A (en) * | 2017-09-15 | 2018-02-27 | 江苏新瑞贝科技股份有限公司 | A kind of enzyme pretreating reagent and processing method for cotton fiber |
| CN114317364B (en) * | 2021-12-30 | 2023-06-13 | 中国科学院青岛生物能源与过程研究所 | Geobacillus altitudinalis and application thereof in production of high-stability alkaline pectase |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1177003A (en) * | 1997-03-19 | 1998-03-25 | 武汉大学 | Novel basophilic Bacillus strain and application in degumming of ramie |
| JP2001061473A (en) * | 1999-08-30 | 2001-03-13 | Agency Of Ind Science & Technol | New polygalacturonase |
-
2005
- 2005-05-12 CN CNB2005100400293A patent/CN100384992C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1177003A (en) * | 1997-03-19 | 1998-03-25 | 武汉大学 | Novel basophilic Bacillus strain and application in degumming of ramie |
| JP2001061473A (en) * | 1999-08-30 | 2001-03-13 | Agency Of Ind Science & Technol | New polygalacturonase |
Non-Patent Citations (2)
| Title |
|---|
| 碱性果胶酶及其在棉纺织预处理中的应用. 董云舟等.工业微生物，第34卷第2期. 2004 |
| 碱性果胶酶及其在棉纺织预处理中的应用. 董云舟等.工业微生物，第34卷第2期. 2004 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1699563A (en) | 2005-11-23 |
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Assignee: Hangzhou Jiangnan Scientific Research Institute Co., Ltd. Assignor: Jiangnan University Contract fulfillment period: 2009.8.20 to 2016.8.19 contract change Contract record no.: 2009330002715 Denomination of invention: Method for improving stability of alkaline pectase in cotton spinning and refining process Granted publication date: 20080430 License type: Exclusive license Record date: 20091030 |
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Free format text: EXCLUSIVE LICENSE; TIME LIMIT OF IMPLEMENTING CONTACT: 2009.8.20 TO 2016.8.19; CHANGE OF CONTRACT Name of requester: HANGZHOU JIANGNAN SCIENCE INSTITUTE CO., LTD. Effective date: 20091030 |
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Granted publication date: 20080430 Termination date: 20140512 |