CN100571564C - Enzyme-aided pearl glossing process - Google Patents
Enzyme-aided pearl glossing process Download PDFInfo
- Publication number
- CN100571564C CN100571564C CNB2005100503529A CN200510050352A CN100571564C CN 100571564 C CN100571564 C CN 100571564C CN B2005100503529 A CNB2005100503529 A CN B2005100503529A CN 200510050352 A CN200510050352 A CN 200510050352A CN 100571564 C CN100571564 C CN 100571564C
- Authority
- CN
- China
- Prior art keywords
- pearl
- enzyme
- aided
- enzyme preparation
- whitening agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title claims abstract description 22
- 230000008569 process Effects 0.000 title claims abstract description 17
- 102000004190 Enzymes Human genes 0.000 claims abstract description 57
- 108090000790 Enzymes Proteins 0.000 claims abstract description 57
- 238000002360 preparation method Methods 0.000 claims abstract description 38
- 239000006081 fluorescent whitening agent Substances 0.000 claims abstract description 31
- 239000000243 solution Substances 0.000 claims abstract description 26
- VQLYBLABXAHUDN-UHFFFAOYSA-N bis(4-fluorophenyl)-methyl-(1,2,4-triazol-1-ylmethyl)silane;methyl n-(1h-benzimidazol-2-yl)carbamate Chemical compound C1=CC=C2NC(NC(=O)OC)=NC2=C1.C=1C=C(F)C=CC=1[Si](C=1C=CC(F)=CC=1)(C)CN1C=NC=N1 VQLYBLABXAHUDN-UHFFFAOYSA-N 0.000 claims abstract description 21
- 238000005282 brightening Methods 0.000 claims abstract description 18
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 10
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical class [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims abstract description 9
- 239000007853 buffer solution Substances 0.000 claims abstract description 6
- 238000011049 filling Methods 0.000 claims abstract description 4
- 239000000872 buffer Substances 0.000 claims abstract description 3
- 229940088598 enzyme Drugs 0.000 claims description 51
- 108091005804 Peptidases Proteins 0.000 claims description 48
- 239000004365 Protease Substances 0.000 claims description 37
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical group OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 33
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 29
- 235000019419 proteases Nutrition 0.000 claims description 29
- 241000228212 Aspergillus Species 0.000 claims description 19
- 102000035195 Peptidases Human genes 0.000 claims description 19
- 235000019833 protease Nutrition 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 150000001875 compounds Chemical class 0.000 claims description 17
- 239000007788 liquid Substances 0.000 claims description 13
- 102000004882 Lipase Human genes 0.000 claims description 11
- 108090001060 Lipase Proteins 0.000 claims description 11
- 239000004367 Lipase Substances 0.000 claims description 10
- 235000019421 lipase Nutrition 0.000 claims description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 9
- 108090000526 Papain Proteins 0.000 claims description 9
- 235000019834 papain Nutrition 0.000 claims description 8
- 229940055729 papain Drugs 0.000 claims description 8
- 102000004169 proteins and genes Human genes 0.000 claims description 7
- 108090000623 proteins and genes Proteins 0.000 claims description 7
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 6
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 6
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 6
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 claims description 6
- 230000009286 beneficial effect Effects 0.000 claims description 6
- 239000000049 pigment Substances 0.000 claims description 6
- 230000001925 catabolic effect Effects 0.000 claims description 5
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical group [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 5
- 230000007935 neutral effect Effects 0.000 claims description 5
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 5
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 4
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 4
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 claims description 4
- -1 carrene Chemical compound 0.000 claims description 4
- JHIVVAPYMSGYDF-UHFFFAOYSA-N cyclohexanone Chemical compound O=C1CCCCC1 JHIVVAPYMSGYDF-UHFFFAOYSA-N 0.000 claims description 4
- 238000011010 flushing procedure Methods 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 4
- ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 claims description 4
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 239000003960 organic solvent Substances 0.000 claims description 3
- 239000002904 solvent Substances 0.000 claims description 3
- OFKABAMEQKYPLK-UHFFFAOYSA-N 1-butoxybutan-1-ol Chemical compound CCCCOC(O)CCC OFKABAMEQKYPLK-UHFFFAOYSA-N 0.000 claims description 2
- ZWEHNKRNPOVVGH-UHFFFAOYSA-N 2-Butanone Chemical compound CCC(C)=O ZWEHNKRNPOVVGH-UHFFFAOYSA-N 0.000 claims description 2
- ZNQVEEAIQZEUHB-UHFFFAOYSA-N 2-ethoxyethanol Chemical compound CCOCCO ZNQVEEAIQZEUHB-UHFFFAOYSA-N 0.000 claims description 2
- SVONRAPFKPVNKG-UHFFFAOYSA-N 2-ethoxyethyl acetate Chemical compound CCOCCOC(C)=O SVONRAPFKPVNKG-UHFFFAOYSA-N 0.000 claims description 2
- XHMWPVBQGARKQM-UHFFFAOYSA-N 3-ethoxy-1-propanol Chemical compound CCOCCCO XHMWPVBQGARKQM-UHFFFAOYSA-N 0.000 claims description 2
- CNGYZEMWVAWWOB-VAWYXSNFSA-N 5-[[4-anilino-6-[bis(2-hydroxyethyl)amino]-1,3,5-triazin-2-yl]amino]-2-[(e)-2-[4-[[4-anilino-6-[bis(2-hydroxyethyl)amino]-1,3,5-triazin-2-yl]amino]-2-sulfophenyl]ethenyl]benzenesulfonic acid Chemical compound N=1C(NC=2C=C(C(\C=C\C=3C(=CC(NC=4N=C(N=C(NC=5C=CC=CC=5)N=4)N(CCO)CCO)=CC=3)S(O)(=O)=O)=CC=2)S(O)(=O)=O)=NC(N(CCO)CCO)=NC=1NC1=CC=CC=C1 CNGYZEMWVAWWOB-VAWYXSNFSA-N 0.000 claims description 2
- DKPFZGUDAPQIHT-UHFFFAOYSA-N Butyl acetate Natural products CCCCOC(C)=O DKPFZGUDAPQIHT-UHFFFAOYSA-N 0.000 claims description 2
- NTIZESTWPVYFNL-UHFFFAOYSA-N Methyl isobutyl ketone Chemical compound CC(C)CC(C)=O NTIZESTWPVYFNL-UHFFFAOYSA-N 0.000 claims description 2
- 238000004140 cleaning Methods 0.000 claims description 2
- 229940028356 diethylene glycol monobutyl ether Drugs 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- 239000012530 fluid Substances 0.000 claims description 2
- FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 claims description 2
- JCGNDDUYTRNOFT-UHFFFAOYSA-N oxolane-2,4-dione Chemical compound O=C1COC(=O)C1 JCGNDDUYTRNOFT-UHFFFAOYSA-N 0.000 claims description 2
- 230000003139 buffering effect Effects 0.000 claims 1
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 abstract description 16
- 239000013078 crystal Substances 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 4
- 230000000975 bioactive effect Effects 0.000 abstract description 3
- 230000007062 hydrolysis Effects 0.000 abstract description 3
- 238000006460 hydrolysis reaction Methods 0.000 abstract description 3
- 230000009467 reduction Effects 0.000 abstract description 3
- 239000011049 pearl Substances 0.000 description 71
- 238000006243 chemical reaction Methods 0.000 description 12
- 239000008363 phosphate buffer Substances 0.000 description 11
- 108091005508 Acid proteases Proteins 0.000 description 8
- 238000004061 bleaching Methods 0.000 description 6
- 229910000019 calcium carbonate Inorganic materials 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 102000011782 Keratins Human genes 0.000 description 5
- 108010076876 Keratins Proteins 0.000 description 5
- 238000000354 decomposition reaction Methods 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 5
- 239000002932 luster Substances 0.000 description 5
- YTPLMLYBLZKORZ-UHFFFAOYSA-N Thiophene Chemical compound C=1C=CSC=1 YTPLMLYBLZKORZ-UHFFFAOYSA-N 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 230000002708 enhancing effect Effects 0.000 description 4
- 239000012467 final product Substances 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 4
- 239000007983 Tris buffer Substances 0.000 description 3
- 239000003513 alkali Substances 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- GPRLSGONYQIRFK-UHFFFAOYSA-N hydron Chemical compound [H+] GPRLSGONYQIRFK-UHFFFAOYSA-N 0.000 description 3
- 229910052742 iron Inorganic materials 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 229910052708 sodium Inorganic materials 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- 239000003643 water by type Substances 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- QGBSISYHAICWAH-UHFFFAOYSA-N dicyandiamide Chemical compound NC(N)=NC#N QGBSISYHAICWAH-UHFFFAOYSA-N 0.000 description 2
- ZUOUZKKEUPVFJK-UHFFFAOYSA-N diphenyl Chemical compound C1=CC=CC=C1C1=CC=CC=C1 ZUOUZKKEUPVFJK-UHFFFAOYSA-N 0.000 description 2
- 210000002615 epidermis Anatomy 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 125000002971 oxazolyl group Chemical group 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- PUZPDOWCWNUUKD-UHFFFAOYSA-M sodium fluoride Chemical compound [F-].[Na+] PUZPDOWCWNUUKD-UHFFFAOYSA-M 0.000 description 2
- 229910052712 strontium Inorganic materials 0.000 description 2
- 229930192474 thiophene Natural products 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- VAJVDSVGBWFCLW-UHFFFAOYSA-N 3-Phenyl-1-propanol Chemical compound OCCCC1=CC=CC=C1 VAJVDSVGBWFCLW-UHFFFAOYSA-N 0.000 description 1
- 244000003416 Asparagus officinalis Species 0.000 description 1
- 235000005340 Asparagus officinalis Nutrition 0.000 description 1
- 241000131386 Aspergillus sojae Species 0.000 description 1
- 102100035606 Beta-casein Human genes 0.000 description 1
- 241000237519 Bivalvia Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 240000006432 Carica papaya Species 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 108010059378 Endopeptidases Proteins 0.000 description 1
- 102000005593 Endopeptidases Human genes 0.000 description 1
- 101000947120 Homo sapiens Beta-casein Proteins 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- DYUQAZSOFZSPHD-UHFFFAOYSA-N Phenylpropyl alcohol Natural products CCC(O)C1=CC=CC=C1 DYUQAZSOFZSPHD-UHFFFAOYSA-N 0.000 description 1
- 101710180319 Protease 1 Proteins 0.000 description 1
- 101710180316 Protease 2 Proteins 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 101710137710 Thioesterase 1/protease 1/lysophospholipase L1 Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 125000005605 benzo group Chemical group 0.000 description 1
- 239000011942 biocatalyst Substances 0.000 description 1
- 230000033558 biomineral tissue development Effects 0.000 description 1
- 239000004305 biphenyl Substances 0.000 description 1
- 235000010290 biphenyl Nutrition 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229940099352 cholate Drugs 0.000 description 1
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000002478 diastatic effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 239000000413 hydrolysate Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 150000004032 porphyrins Chemical class 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
- 235000021067 refined food Nutrition 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000010944 silver (metal) Substances 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 239000011775 sodium fluoride Substances 0.000 description 1
- 235000013024 sodium fluoride Nutrition 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- CIOAGBVUUVVLOB-UHFFFAOYSA-N strontium atom Chemical compound [Sr] CIOAGBVUUVVLOB-UHFFFAOYSA-N 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 125000000999 tert-butyl group Chemical group [H]C([H])([H])C(*)(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Cosmetics (AREA)
Abstract
The present invention relates to a kind of pearl aftertreatment technology, a kind ofly handle pearl surface by bioactive enzyme and improve nacreous treatment process thereby relate in particular to; May further comprise the steps: a. preliminary treatment; B. dispose buffer solution or saturated sodium carbonate solution: the c. enzyme preparation is added lustre to: the non-gloss that pearl aragonite crystal is produced that steps such as d. fluorescent whitening agent light filling promptly utilize the biocatalysis hydrolysis of biology enzyme in buffer environment to remove attached to the pearl surface transmits better; The combined with fluorescent brightening agent makes the required time that adds lustre to shorten to a week, and the centre need not be changed system again.Significantly the reduction of erection time, save cost.
Description
Technical field
The present invention relates to a kind of pearl aftertreatment technology, a kind ofly handle pearl surface by bioactive enzyme and improve nacreous treatment process thereby relate in particular to.
Background technology
Pearl is the outer embrane cell of some shellfish of mollusk Bivalvia secreted a kind of biomineralization tissue in metabolic process, having unique crystal structure, is a concentric spheroid that is formed by crystallization of thousands of layers of calcium carbonate aragonitic and the orderly at interval arrangement of shell keratin interlaminate.The chemical analysis of pearl mainly is inorganic compositions such as calcium carbonate, be aided with the shell keratin, water, wherein containing trace element has S, P, Na, Mg, Al, Fe, Cu, Ag, Ti, K, Sr etc., and amino acid has lucid asparagus, Soviet Union, silk, paddy, sweet, third, half Guang, figured silk fabrics, egg, different bright, bright, junket, phenylpropyl alcohol, relies, group, smart, auxilliary, look etc.Pearl has the feature of all Gem Studies: promptly specific color, gloss, color and luster, transparency, shape and size, density, hardness, index of refraction and flaw
12There is research to point out: the chemical composition of pearl, trace element, and quality such as the content of organic substance and pearl color are closely related, and minor metallic element iron, manganese etc. mainly are to form the form existence of complicated complex compound (porphyrin etc.) with organic matter or to be adsorbed by organic substance.In being in all the time, pearl in the environment utricule, is capsule liquid parcel in forming process.Its outer surface also has other lipid, albumen composition as film except keratin.The gloss of pearl mainly by the physical arrangement decision of calcium carbonate aragonite crystal, is difficult to the method improvement with chemistry, physics on this level; But materials such as lipid that its skin adheres to and albumen can influence seeing through of light and reflection and refraction, thereby influence the gloss of pearl.So can handle the pearl surface with protease or lipase, reach light enhancing effect.
Summary of the invention
Thereby the object of the present invention is to provide a kind of bioactive enzyme that adopts pearl to be handled the production technology that reaches pearl brightening.
Solution of the present invention is based on the inventor by long experimental study, utilize the characteristic of the distinctive biocatalyst of biology enzyme, and enzyme in processing procedure to the requirement of processing environment, draw and a kind of pearl had light enhancing effect, but do not damage the treatment process of pearl again.
Above-mentioned technical problem of the present invention is finished by the following technical programs:
A kind of enzyme-aided pearl glossing process may further comprise the steps:
A. preliminary treatment:
(1) classification is promptly carried out rough classification by thickness, color, gloss, form and the big wisp pearl of nacre, so that handle respectively;
(2) surface preparation wraps in gauze with pearl and is built in heat treated in the water-bath, and temperature generally is controlled at about 55 ℃, continues 3 hours to 80 hours, makes most of pigment be beneficial to bleaching in decomposition;
B. dispose buffer solution or saturated sodium carbonate solution: will cushion solute and add in the entry configuration to make (by mass percentage) be 0.1%～1.0% buffer solution; Wherein the pH value of cushioning liquid be 6.0～10.0 or the configuration saturated sodium carbonate solution;
C. enzyme preparation is added lustre to: above-mentioned cushioning liquid is joined in the glass container, add pearl, the enzyme preparation of (by mass percentage) 0.05～2%; Take out after 3～15 hours in water-bath under 35～65 ℃ the constant temperature; After washing 2～5 times, dry; Or enzyme preparation that will (by mass percentage) 0.05～2% joins in the glass container, adjusts pH value to 6.0～10.0 with saturated sodium carbonate solution, adds pearl, takes out after 3～7 hours in water-bath under 40～65 ℃ the constant temperature; After washing 2～5 times, dry;
D. fluorescent whitening agent light filling: it is 0.05%～1.0% fluorescent brightening system that above-mentioned pearl of drying is put into concentration, places lighting box, and room temperature was taken out after 5～10 days, after the cleaning fluid flushing, dried.
As preferably, described cushioning liquid is phosphate buffer, and solute is dipotassium hydrogen phosphate and potassium dihydrogen phosphate, and wherein the concentration of cushioning liquid (by mass percentage) is 0.1%～1.0%.
The inventor gets dipotassium hydrogen phosphate and potassium dihydrogen phosphate is dissolved in water, preparation phosphate buffer (pH=8.5).In conical flask 1, add phosphate buffer, 30 pearls; In conical flask 2, add phosphate buffer, 30 pearls and enzyme preparation (1%); In water bath with thermostatic control 5 hours, the dereaction of inclining liquid, water is rinsed well repeatedly, and the pearl of bottle in 2 be bright than in the bottle 1 obviously, and white slightly.And pearl and former pearl are than obviously dark matt in the conical flask 1, and bottle 2 pearls are brighter slightly than former pearl, and are white, and be more inferior than then with the commodity pearl of adding lustre to.
The inventor gets Pehanorm again and adds stirring and dissolving, and is diluted to 1000ml, regulates pH to 8.0 with the 6mol/L hydrochloric acid solution and promptly gets the tris buffer solution.
In bottle 1, add 50 ml phosphate buffers and 30 pearls; In bottle 2, add 50 milliliters of tris buffer solutions and restrain enzyme preparation (1%) with 30 pearls and 0.5.In 53 ℃ of waters bath with thermostatic control 5 hours, the dereaction of inclining liquid, water is rinsed well repeatedly.The pearl of bottle in 2 with use phosphate buffer not compare obviously to improve, illustrate that use phosphate buffered liquor ratio tris buffer solution is more suitable for enhanced shine to pearl as the catalytic environment of enzyme preparation.
As preferably: described enzyme preparation (by mass percentage) is 0.05～4% compound protease, and wherein the enzyme preparation temperature of adding lustre to is 40～55 ℃, and 4～5 hours time that enzyme preparation is added lustre to, the add lustre to pH value of solution of enzyme preparation is 6.0～10.0.Compound protease: optimal reaction temperature is 40～55 degrees centigrade, and optimal pH is 6.0～10.0, usually addition (mass fraction): 0.01～0.5%.Papain, Aspergillus proteinase, neutrality and alkalescent protease that this enzyme contains equivalent (be may I ask here and could be used code name? such as protease 1, protease 2 or the like, cannot be insufficient because of occurring like this disclosing), have the ability of very strong aminosal.
As preferably, described compound protease is that two or more papain, Aspergillus proteinase, lipase, neutral protein catabolic enzyme, acid protease, alkalescent protease formed, and wherein must contain Aspergillus proteinase.
Papain: optimal reaction temperature is 50～65 degree, and optimal pH is 6.0～7.0, addition (mass fraction): 0.2～0.6%.This enzyme adopts biotechnology to refine from papaya plant green fruit and forms.Form by 212 amino acid, molecular weight 21000, belong to and contain the sulfydryl endopeptidase, activity with protease and lipase, specificity is widely arranged, animal/vegetable protein, polypeptide, acid amides etc. by stronger enzymolysis ability, are widely used in food, medicine, daily use chemicals, feed, leather and textile industry.
Aspergillus proteinase: optimal reaction temperature is 50～55 degree, and optimal pH is 7.0～10.0.Make through operations such as liquid deep layer fermenting, extractions by the Aspergillus sojae protease strain, contain neutrality and alkali protease, have the ability of very strong aminosal.
Lipase: optimal reaction temperature is short time 40～42 degree, long-time 38～40 degree, optimal pH 7.0～7.5.Effect ' RCO-OR ', main product: lipid---aliphatic acid, glycerine and other incomplete hydrolysate.Heavy metal ion copper, iron and sodium fluoride and aliphatic acid have inhibitory action to enzyme, and calcium, strontium and cholate have activation to it.
The neutral protein catabolic enzyme: optimal reaction temperature is 45～50 degree, optimal pH 5.0～7.0.Addition (mass fraction): 0.00025～0.01%.Be mainly based on neutral proteinase and contain diastatic complex enzyme, can improve bread the quality of production, enhance productivity.Utilize vegeto-animal protein raw materials, make all kinds of processed foods, flavouring etc.Going up biomedical high-tech development corporation, Ltd. of marine section produces.
Acid protease (sumizyme AP Japan): optimal reaction temperature 40 degree, optimal pH 4.0, addition (mass fraction) 0.1%.
Acid protease (homemade): optimal reaction temperature 40～50 degree, optimal pH 6.0, addition (mass fraction): 0.1%.Shanghai CASB Biotechnology Co., Ltd (Shanghai Research Center of Biotechnology) development.
The alkalescent protease: optimal reaction temperature is 45～50 degree, optimal pH 6.0～9.0, addition (mass fraction): 0.08～0.12%.Can decompose with the animal/vegetable protein in neutrality or the alkalescent scope is the protein of raw material.Going up biomedical high-tech development corporation, Ltd. of marine section provides.
Based on existing various bleaching shining methods, chemical property in conjunction with available various protease and lipase (comprising papain, Aspergillus proteinase, lipase, neutral protein catabolic enzyme, acid protease, alkalescent protease), we at first design and have prepared compound protease, investigate the validity of protease to pearl brightening.Experimental result shows that the long-time pearl of soaking obviously tarnishes in pure phosphate buffer, and if add compound protease in this buffer solution, then the gloss of pearl can keep fully, even slight increase is arranged.Infer that thus compound protease or a certain protease wherein play enhanced shine to the pearl surface.
As preferably, described enzyme preparation (by mass percentage) is 0.01～1.0% Aspergillus proteinase, and wherein the enzyme preparation temperature of adding lustre to is 40～65 ℃, and 4～5 hours time that enzyme preparation is added lustre to, the add lustre to pH value of solution of enzyme preparation is 7.0～10.0.
In conjunction with the optimal reaction temperature and the pH value of various enzymes, the phosphate system of selected pH 7.8 is a cushioning liquid, to compound protease, and papain, Aspergillus proteinase, the alkalescent protease, neutral protein catabolic enzyme and lipase screen, and the result is as follows:
Table 1
| Enzyme | Aspergillus proteinase | Compound protease | Neutral proteinase | Papain | Lipase | Alkali protease |
| Gloss changes | + | + | 0 | 0 | - | - |
Experimental result shows that compound protease and Aspergillus proteinase have tangible enhanced shine to pearl.And with regard to compound protease, when pH7.8, compare with pH8.5 last time, light enhancing effect is better.Because contain Aspergillus proteinase and the invalid enzyme of other proof in the compound protease prescription, so the component that wherein works should be Aspergillus proteinase.Under this reaction condition, independent neutral proteinase and independent papain do not have obvious effect.And independent lipase can make the pearl surface more tarnish, and may be because the little fat of former bead surface has been removed in its hydrolysis, influences gloss thereby the permeability of light is changed.As for independent alkali protease, may be that its strong excessively protein hydrolysate ability is damaged the keratic complete structure of shell.In addition, two kinds of acid proteases have been eliminated in independent reaction.Because pearl mainly is made up of aragonite crystal and keratin, hydrogen ion meeting and calcium carbonate react under the acid condition, have changed the internal structure of pearl, make pearl that serious " peeling " phenomenon take place.So acid protease is inadvisable.Handle pearl with acid protease (sumizyme AP Japan), find in the thermostatic process that the pearl epidermis heaves, in have bubble to generate, and epidermis is complete.Show under the acid condition hydrogen ion can and the calcium carbonate generation carbon dioxide that reacts, complete not being damaged of adventitia illustrates that this enzyme is inoperative to keratin.Do not see in the homemade acid protease constant temperature processing procedure that bubble generates, present the frosted shape but handle pearl surface, back, feel is coarse.Illustrate that this kind enzyme carries out through film and calcium carbonate reaction simultaneously to pearl keratic destruction in surface and hydrogen ion.
As preferably, described fluorescent brightening system (by mass percentage) is 0.01%～2.0% water soluble fluorescence brightening agent.
As preferably, described water soluble fluorescence brightening agent is fluorescent whitening agent PRS (4,4 '-two [(6-anilino--4-methoxyl group-1,3,5-triazines-2-yl) amino] talan-2,2 '-sodium disulfonate), fluorescent whitening agent VBL (4,4 '-two [(4-hydroxyethylamine-6-anilino--1,3,5-triazines-2-yl) amino] talan-2,2 '-sodium disulfonate), a kind of in the fluorescent brightener CBS (4,4-two (2-two yellow acid styryls) biphenyl).
As preferably, described fluorescent brightening system (by mass percentage) is 0.1～0.4% oil-soluble fluorescent whitening agent, and wherein solvent is a kind of in methyl alcohol, ethanol, ethylene glycol, acetone, ether, ethyl acetate, DAA, 2-butanone, MIBK, butanediol monobutyl ether, diethylene glycol monobutyl ether, ethylene glycol monoethyl ether, toluene, dimethylbenzene, hydroxypropyl ethylether, isopropyl alcohol, cyclohexanone, carrene, n-butanol, isobutanol, ethyl cellosolve acetate, butyl acetate, the n-hexane organic solvent.
As preferably, described oil-soluble fluorescent whitening agent is fluorescent whitening agent OB (two (the 5-tert-butyl group-2-benzo mouth oxazolyl) thiophene of 2.5-), fluorescent whitening agent PF (two (5-methyl-2-benzo mouth the oxazolyl)-ethene of 1.2-), fluorescent whitening agent PB (a kind of in 2.5-two-(the benzo mouth oxazole-2-) thiophene).
Through the pearl that enzyme was handled, its gloss obviously strengthens, but still has a certain distance with the commodity pearl ratio that adds lustre to.For further strengthening pearly-lustre, also need to carry out light filling with fluorescent whitening agent.Fluorescent whitening agent is divided into two big classes according to dissolubility, water soluble fluorescence brightening agent such as fluorescent whitening agent PRS, oil-soluble fluorescent whitening agent such as fluorescent whitening agent OB, PRS is water-soluble with fluorescent whitening agent, fluorescent whitening agent OB is dissolved in the organic solvents such as methyl alcohol, ethanol, ethylene glycol, add pearl respectively, place lighting box.Found that the methanol solution best results of fluorescent whitening agent OB.(table 2)
Table 2
After soaking in the solution of water soluble fluorescence brightening agent PRS, the pearl outer surface part is enclosed one deck white films, and adhesion-tight, and pearl has increased original gloss a little.It is tighter to analyze reason and may be ionic brightening agent PRS absorption, and for fluorescent whitening agent OB, experiment finds that solvent polarity is strong more, and then light enhancing effect is good more.
The current method of adding lustre to is to use fluorescent whitening agent OB in the pearl processing now, cooperates different complicated dicyandiamide solutions, gets through long-time the immersion.Need during this time to change dicyandiamide solution repeatedly, the time is long, and workload is big.Use Aspergillus proteinase combined with fluorescent brightening agent, required time shortens to a week, and system need not be changed in the centre.Significantly the reduction of erection time, save cost
Therefore the present invention has the following advantages:
The non-gloss that pearl aragonite crystal is produced that the present invention utilizes the biocatalysis hydrolysis of biology enzyme in buffer environment to remove attached to the pearl surface transmits better; The combined with fluorescent brightening agent makes the required time that adds lustre to shorten to a week, and the centre need not be changed system again.Significantly the reduction of erection time, save cost.
Specific embodiments
Below by embodiment, technical scheme of the present invention is described in further detail.
Embodiment 1
(1) classification is promptly carried out rough classification by thickness, color, gloss, form and the big wisp pearl of nacre, so that handle respectively.
(2) surface preparation wraps in gauze with pearl and is built in heat treated in the water-bath, and temperature generally is controlled at 50 ℃, continues 80 hours, makes most of pigment be beneficial to bleaching in decomposition;
Get dipotassium hydrogen phosphate 5.59g and potassium dihydrogen phosphate 0.41g is dissolved in water to 1000ml, preparation phosphate buffer (pH=8.5).In conical flask, add the 50ml phosphate buffer, 30 pearls, 0.5 gram compound protease (1%); In 35 ℃ of waters bath with thermostatic control 15 hours, the dereaction of inclining liquid, water is rinsed well repeatedly, and fluorescent whitening agent OB 2.5 grams are added in the 1250ml methyl alcohol, and ultrasonic wave is handled and is made dissolving fully.Pearl 1000 grams that enzyme was handled are put into this system, place lighting box, room temperature was taken out after 5 days, used an amount of washed with methanol, dried to get final product.Pearly luster is strong.
Embodiment 2
(1) classification is promptly carried out rough classification by thickness, color, gloss, form and the big wisp pearl of nacre, so that handle respectively.
(2) surface preparation wraps in gauze with pearl and is built in heat treated in the water-bath, and temperature generally is controlled at about 55 ℃, continues 30 hours, makes most of pigment be beneficial to bleaching in decomposition;
Take by weighing Aspergillus proteinase 1.25 grams, with 1250mL deionized water dissolving (pH 6.4), 1.3mL transfers to 6.0 with pH value of solution with saturated sodium carbonate solution.1000 gram pearls are put into this Aspergillus proteinase solution, and 40 ℃ of water-baths were taken out after 7 hours, and flushing is dried repeatedly.Fluorescent whitening agent OB 2.5 grams are added in the 1250ml methyl alcohol, and ultrasonic wave is handled and is made dissolving fully.Pearl 1000 grams that enzyme was handled are put into this system, place lighting box, room temperature was taken out after 7 days, used an amount of washed with methanol, dried to get final product.Pearly luster is strong.
Embodiment 3
(1) classification is promptly carried out rough classification by thickness, color, gloss, form and the big wisp pearl of nacre, so that handle respectively.
(2) surface preparation wraps in gauze with pearl and is built in heat treated in the water-bath, and temperature generally is controlled at about 60 ℃, continues 3 hours, makes most of pigment be beneficial to bleaching in decomposition;
Get dipotassium hydrogen phosphate 7.59g and potassium dihydrogen phosphate 2.41g is dissolved in water to 1000ml, preparation phosphate buffer (pH=7.5).In conical flask, add the 50ml phosphate buffer, 30 pearls, 1.0 gram Aspergillus proteinases (2%); In 53 ℃ of waters bath with thermostatic control 5 hours, the dereaction of inclining liquid, water is rinsed well repeatedly, and fluorescent whitening agent PRS 1.25 grams are added in the 1250ml deionized water, and ultrasonic wave is handled and is made dissolving fully.Pearl 1000 grams that enzyme was handled are put into this system, place lighting box, room temperature was taken out after 10 days, used an amount of washed with methanol, dried to get final product.Pearly luster is stronger.
Embodiment 4
(1) classification is promptly carried out rough classification by thickness, color, gloss, form and the big wisp pearl of nacre, so that handle respectively.
(2) surface preparation wraps in gauze with pearl and is built in heat treated in the water-bath, and temperature generally is controlled at about 55 ℃, continues 7 hours 7, makes most of pigment be beneficial to bleaching in decomposition;
Take by weighing compound protease 1.25 grams, with 1250mL deionized water dissolving (pH 6.4), 1.3mL transfers to 7.0 with pH value of solution with saturated sodium carbonate solution.1000 gram pearls are put into this compound protein enzyme solutions, and 50 ℃ of water-baths were taken out after 4 hours, and flushing is dried repeatedly.
Fluorescent whitening agent PB25 gram is added in the 1250ml methyl alcohol, and ultrasonic wave is handled and is made dissolving fully.Pearl 1000 grams that enzyme was handled are put into this system, place lighting box, room temperature was taken out after 5 days, used an amount of washed with methanol, dried to get final product.Pearly luster is strong.
Specific embodiment described herein only is that the present invention's spirit is illustrated.The technical staff of the technical field of the invention can make various modifications or replenishes or adopt similar mode to substitute described specific embodiment, but can't depart from spirit of the present invention or surmount the defined scope of appended claims.
Claims (9)
1. enzyme-aided pearl glossing process may further comprise the steps:
A. preliminary treatment:
(1) classification is promptly carried out rough classification by thickness, color, gloss, form and the big wisp pearl of nacre, so that handle respectively;
(2) surface preparation wraps in gauze with pearl and is built in heat treated in the water-bath, and temperature is controlled at 50～60 ℃, continues 3 hours to 80 hours, most of pigment is decomposed be beneficial to add lustre to;
B. dispose buffer solution or saturated sodium carbonate solution: will cushioning solute, to add in the entry that configuration makes be 0.1%～1.0% buffer solution by mass percentage; Wherein the pH value of cushioning liquid be 6.0～10.0 or the configuration saturated sodium carbonate solution;
C. enzyme preparation is added lustre to: above-mentioned cushioning liquid is joined in the glass container, add pearl, add the enzyme preparation of mass percent 0.05%～2%; Water-bath was taken out after 3～15 hours under a constant temperature that is between 35～65 ℃; After washing 2～5 times, dry;
Or 0.05%～2% enzyme preparation joins in the glass container by mass percentage, adjusts pH value to 6.0～10.0 with saturated sodium carbonate solution, adds pearl, and water-bath was taken out after 3～7 hours under a constant temperature that is between 40～65 ℃; After washing 2～5 times, dry;
Above-mentioned enzyme preparation contains Aspergillus proteinase;
D. fluorescent whitening agent light filling: it is 0.05%～1.0% fluorescent brightening system that above-mentioned pearl of drying is put into concentration, places lighting box, and room temperature was taken out after 5～10 days, after the cleaning fluid flushing, dried.
2. enzyme-aided pearl glossing process according to claim 1 is characterized in that, described buffering solute is dipotassium hydrogen phosphate and potassium dihydrogen phosphate, and described buffer concentration is 0.1%～1.0% by mass percentage.
3. enzyme-aided pearl glossing process according to claim 1 and 2, it is characterized in that, described enzyme preparation is that mass percent is 0.05%～2% compound protein enzyme preparation, wherein the enzyme preparation temperature of adding lustre to is 40～55 ℃, 4～5 hours time that enzyme preparation is added lustre to, the add lustre to pH value of solution of enzyme preparation is 6.0～10.0.
4. enzyme-aided pearl glossing process according to claim 3, it is characterized in that, described compound protease is made of at least a enzyme in Aspergillus proteinase and following group, described group by papain, lipase, and the neutral protein catabolic enzyme except above-mentioned papain, Aspergillus proteinase, lipase, acid protease, alkalescent protease constitute.
5. enzyme-aided pearl glossing process according to claim 1, it is characterized in that, described enzyme preparation is 0.05%～1.0% Aspergillus proteinase preparation by mass percentage, wherein the enzyme preparation temperature of adding lustre to is 40～65 ℃, 4～5 hours time that enzyme preparation is added lustre to, the add lustre to pH value of solution of enzyme preparation is 7.0～10.0.
6, enzyme-aided pearl glossing process according to claim 1 is characterized in that, described fluorescent brightening system is that mass percent is 0.1～1.0% water soluble fluorescence brightening agent.
7. enzyme-aided pearl glossing process according to claim 6 is characterized in that, described water soluble fluorescence brightening agent is a kind of in fluorescent whitening agent PRS, fluorescent whitening agent VBL, the fluorescent brightener CBS.
8. enzyme-aided pearl glossing process according to claim 1, it is characterized in that, described fluorescent brightening system is that mass percent is 0.1%～0.4% oil-soluble fluorescent whitening agent, and wherein solvent is a methyl alcohol, ethanol, ethylene glycol, acetone, ethyl acetate, ether, DAA, the 2-butanone, MIBK, the butanediol monobutyl ether, diethylene glycol monobutyl ether, ethylene glycol monoethyl ether, toluene, dimethylbenzene, the hydroxypropyl ethylether, isopropyl alcohol, cyclohexanone, carrene, n-butanol, isobutanol, ethyl cellosolve acetate, butyl acetate, a kind of in the n-hexane organic solvent.
9. enzyme-aided pearl glossing process according to claim 8 is characterized in that, described oil-soluble fluorescent whitening agent is a kind of among fluorescent whitening agent OB, fluorescent whitening agent PF, the fluorescent whitening agent PB.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100503529A CN100571564C (en) | 2005-05-18 | 2005-05-18 | Enzyme-aided pearl glossing process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100503529A CN100571564C (en) | 2005-05-18 | 2005-05-18 | Enzyme-aided pearl glossing process |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1864579A CN1864579A (en) | 2006-11-22 |
| CN100571564C true CN100571564C (en) | 2009-12-23 |
Family
ID=37423865
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100503529A Active CN100571564C (en) | 2005-05-18 | 2005-05-18 | Enzyme-aided pearl glossing process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100571564C (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101209147B (en) * | 2006-12-28 | 2010-11-10 | 浙江山下湖珍珠集团股份有限公司 | Pearl whitening treatment technique |
| CN104490029B (en) * | 2014-11-19 | 2016-01-20 | 浙江工业大学 | The fast bleaching method of a kind of pearl and bleaching liquid |
| CN105011488B (en) * | 2015-07-02 | 2016-08-31 | 广东海洋大学 | A kind of surface decorating liquid bleaching useless pearl and the method modifying useless pearl thereof |
| CN108652153A (en) * | 2018-04-02 | 2018-10-16 | 浙江天使之泪珍珠股份有限公司 | A kind of neutralisation treatment technique in pearl process procedure |
-
2005
- 2005-05-18 CN CNB2005100503529A patent/CN100571564C/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN1864579A (en) | 2006-11-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100571564C (en) | Enzyme-aided pearl glossing process | |
| CN100553518C (en) | Enzyme-aided pearl bleaching process | |
| Islam et al. | Recent advances in extraction of chitin and chitosan | |
| Ravikumar et al. | A protease from the medicinal mushroom Pleurotus sajor-caju; production, purification and partial characterization | |
| CN104388351A (en) | Preparation method of natural colorless biological mildew-proof anticorrosive bactericide | |
| CN103598400A (en) | Extraction method of aquatic collagen | |
| CN108456261A (en) | The method of shrimp and crab shells steam explosion cleaning refining Poly-generation | |
| Lancia et al. | Adaptive differences in digestive enzyme activity in the crab Neohelice granulata in relation to sex and habitat | |
| CN104894034B (en) | A kind of marine source micromonospora SCSIO 01819, enzymes soln and its preparation method and application | |
| CN101461593A (en) | Combined enzymolysis and solid-liquid biphase bleaching method for bleaching fresh water pearl | |
| CN1321180C (en) | Method for raising stability for preserving catalase prepared by microbiological method | |
| CN102212452A (en) | Method for clarifying yellow rice wine | |
| CN103725738B (en) | The method preparing collagen polypeptide with Carnis Pseudosciaenae leftover bits and pieces | |
| CN1326987C (en) | Technological process of maturing grain spirit | |
| CN101164469A (en) | Method for optimizing pearl color, light and brightness | |
| CN103602605B (en) | A kind of genetic engineering bacterium of high expression aspergillus oryzae prolyl endopeptidase and application thereof | |
| CN111990652B (en) | Glossy ganoderma collagen peptide powder and preparation method thereof | |
| CN104642937A (en) | Production method of iron-rich germinated brown rice | |
| WO2012115409A2 (en) | Method for using an enzyme resistant to high pressures | |
| CN103589741B (en) | A kind of new proline specific endo protease gene and application thereof | |
| CN105603031A (en) | Aquatic protein active peptide preparing method without adopting high-temperature treatment | |
| CN107535775A (en) | One kind is based on biotechnology processing egg biologic beverage technique | |
| CN102293488B (en) | Pearl brightening process | |
| CN105821099A (en) | Process for preparing antioxidant peptides from pinctada martensii by adopting fermentation method | |
| CN105624242A (en) | Method for preparing active peptide through high-temperature fermentation of aquatic protein |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Enzymatic brightening of Pearl Effective date of registration: 20211209 Granted publication date: 20091223 Pledgee: Shanxiahu sub branch of Zhejiang Zhuji Rural Commercial Bank Co.,Ltd. Pledgor: ZHEJIANG RUANS PEARL HOLDING Co.,Ltd. Registration number: Y2021980014509 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20221201 Granted publication date: 20091223 Pledgee: Shanxiahu sub branch of Zhejiang Zhuji Rural Commercial Bank Co.,Ltd. Pledgor: ZHEJIANG RUANS PEARL HOLDING Co.,Ltd. Registration number: Y2021980014509 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Enzymatic brightening process of pearl Effective date of registration: 20221208 Granted publication date: 20091223 Pledgee: Zhejiang Zhuji Rural Commercial Bank Co.,Ltd. Pledgor: ZHEJIANG RUANS PEARL HOLDING Co.,Ltd. Registration number: Y2022980025669 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20240102 Granted publication date: 20091223 Pledgee: Zhejiang Zhuji Rural Commercial Bank Co.,Ltd. Pledgor: ZHEJIANG RUANS PEARL HOLDING Co.,Ltd. Registration number: Y2022980025669 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right |