CN1294990C - Composition of nanometer SOD and ginseng or its extract and its preparation method - Google Patents
Composition of nanometer SOD and ginseng or its extract and its preparation method Download PDFInfo
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Abstract
The present invention relates to the composition of nanometer SOD and ginseng or the extract of ginseng, and a preparation method of the composition. In 1 to 100000 active units of nanometer SOD, 1 to 50 g of nanometer ginseng or the extract of nanometer ginseng is contained, and the grain diameter is smaller than or equal to 10 to 80 nanometers. The combination application of the nanometer composition of the nanometer SOD and the ginseng or the extract of the ginseng enhances the concentration and the effective components of the drug. The anti-oxidation activity is enhanced by more than 10 times, the skin and mucous membrane absorptivity reaches more than 98%, and the half-life is prolonged by more than two orders of magnitude. The composition of nanometer SOD and ginseng or the extract of ginseng can enter cells to directly clear free radicals, and has stronger anti-lipid peroxidation. The comprehensive drug effects are more thoroughly exerted and are much better than the drug effects of the traditional compound Chinese drugs. The composition of nanometer SOD and ginseng or the extract of ginseng is used for first aid and for treating diseases of cardiovascular systems, diseases of the stomach and the liver, diabetes, psychopathy, neurasthenia and impotence. Ginseng contains rich SOD, and is suitable for athletes, emergent patients, and people with external wounds. The absorption speed is high. The drug effect of the composition of nanometer SOD and ginseng or the extract of ginseng is 3 to 5 times higher than that of SOD, and is 5 to 10 times higher than that of ginseng. The composition of nanometer SOD and ginseng or the extract of ginseng is contribution to the human medicine career.
Description
Affiliated field
The present invention relates to the composition and method of making the same of superoxide dismutase SOD and Chinese herbal medicine Radix Ginseng, particularly nanometer SOD and Radix Ginseng or its extract.With the compositions nanorize of SOD and Radix Ginseng or its extract, be to utilize both complementary functions, eliminate unnecessary superoxide anion free radical and hydroxy radical in the body, stabilizing cell membrane and antienzyme activity are used for first aid; The treatment cardiovascular system diseases; Treatment harmonization of the stomach hepatic disease; The treatment diabetes; Treat in psychosis; The treatment neurasthenia; Treatment sexual impotence, itself is rich in SOD Radix Ginseng, can satisfy the needs of human health longevity.
Background technology
The Chinese herbal medicine Radix Ginseng is the root (dry root of Radix Ginseng) of Araliaceae Radix Ginseng.The main root hypertrophy, meat, cylindric, often divide severally, practise title " clever body " or " horizontal body " by root top difference person; Bottom difference person practises title " stupid body " or " along body "; Fibrous root is long, and most small wart-like substances are arranged; The stem trace is arranged on the rhizome, give birth to several adventitious roots sometimes.Be born in the dense woods; Various amino acid contents are respectively 1.6884%, and 0.2342%1.358%0.9435% is rich in necessary aminoacid of human body and drug effect aminoacid, and content of ginsenoside is up to 16.3%; Pharmacology: to neural influence, Radix Ginseng is to the central nervous system, and particularly there is certain specific action at its senior position; To the influence of body characteristic, it is similar that the people participates in Radix Et Caulis Acanthopanacis Senticosi, Radix Schisandrae Bicoloris etc., has the effect of " adaptogen " sample, can enhancing body to the defence capability of various destructive stimuluses; Influence to hormonal system, (1) to hypophysis-interrenal system (two) to gonad: Radix Ginseng does not have gonadotropic Effect, and can be excited hypophysis secretion promoting sexual gland hormone (three) to other endocrine gland: the heavy dose of Radix Ginseng short-term is used, can strengthen the influence of the thyroid function (four) of rabbit, a, to carbohydrate metabolism b, to other substance metabolismes to substance metabolism; To blood circulation influence a, to heart b, to blood pressure; Other effects: Radix Ginseng infusion and erythrocyte effect, can cause variable color and turbidity and precipitation, but haemolysis not; Function cures mainly: strongly invigorating primordial QI, promote the production of body fluid admittedly take off, and calm the nerves, it is deficient to control impairment caused by overstrain, lack of appetite, asthenia, food is told in regurgitation, the stool lingering diarrhea, empty cough with asthma is short, the spontaneous perspiration sudden collapse, palpitation with fear, forgetful, dizziness and headache, sexual impotence, frequent micturition is quenched one's thirst, woman uterine bleeding, and children's's intermittent infantile convulsion, and prolonged deficiency is not multiple, the card of all qi-blood-body fluid deficiencies; Have that strongly invigorating primordial QI, multiple arteries and veins take off admittedly, invigorating the spleen to benefit the lung, promoting the production of body fluid and inducing sedation of the mind effect.Be used for weak body and prostration, the cold extremities faint pulse, insufficiency of the spleen lack of appetite, the deficiency of the lung is breathed with cough, and Tianjin wound is thirsty, and interior-heat is quenched one's thirst, and prolonged illness is empty wins, chi throb with fear insomnia, sexual impotence cold womb, mental and physical efforts fail and exhaust cardiogenic shock; For oral administration: as to fry in shallow oil soup, 4～15 grams, big agent 1.5～50 grams; Also can endure cream, or go into ball, diffusing; Clinical report: be used for first aid; The treatment cardiovascular system diseases; Treatment harmonization of the stomach hepatic disease; The treatment diabetes; The treatment psychosis; The treatment neurasthenia; Treatment sexual impotence.
As a kind of functional component, the physiological function of nanometer SOD can be summarized as: remove and produce excessive ultra-oxygen anion free radical in the body metabolic process, delay owing to free radical is encroached on the aging phenomenon that occurs, as delaying decrepitude of skin and the sedimentary appearance of lipofuscin; Improve the resistance of human body, comprise tumor, inflammation, emphysema, cataract and autoimmune disease etc. inducing an illness owing to the free radical infringement; Improve the resistance of human body to the extraneous incitant of free radical, as smog, radiation, toxic chemical and poisonous pharmaceuticals etc., enhancing body is the adaptive faculty of environment to external world; Pain and the serious adverse of ameliorate tumor patient when carrying out chemotherapy, radiotherapy is as bone marrow injury and leukopenia etc.; Eliminate organism fatigue, strengthen adaptive faculty the over loading large amount of exercise.In vivo, nanometer SOD can influence the structure of organic minute ligand of some non-enzyme, participates in the metabolism of interior various materials of body and energy, thereby demonstrates the physiological function that it has: be the constituent of multiple metalloenzyme in the body; Protection stabilate film; Promote the growth promoter and the tissue regeneration of body; Regulate immunologic function; Influence hormonal system; Improve the sense of taste and promote appetite; Promote the homergy and the visual performance of vitamin A; The normal function of protection skin and skeleton; Promote intelligence development.
The principal element that influences the SOD clinical practice has: biological half-life; The foreign protein immunogenicity; The permeability of cell membrane and stability.After this showed that modification SOD enters the body circulation, it was slack-off to eliminate speed, but distribution speed is still very fast; Compare with free SOD, modification SOD is the content height in liver and kidney, and content is also higher in brain; The free distribution sequence trend of SOD in tissue is stomach, intestinal, muscle, lung, brain, liver, brain, kidney, spleen, the heart and testis, the result proves that variation has taken place modification enzyme's metabolic pathway, this and enzyme cell membrane permeability-related, content during the modification enzyme organizes in brain is higher, illustrate that the modification enzyme can pass through blood brain barrier, this is significant for the treatment cerebral trauma.Mainly concentrate on radioprotective at present both at home and abroad, antitumor, on defying age and the autoimmune disease, the disease kind of treatment has: ischemic reperfusion syndrome, oxygen intoxication, radiation damage, dermatosis, osteoarthritis, rheumatoid arthritis, burn, senile cataract, familial muscular dystrophy etc., because the SOD that uses is heterologous protein at present, and molecular weight is all greater than 30000, therefore human body is the problem of very paying close attention in the application to its toleration, illustrate that SOD has no side effect, the people has stronger tolerance to it; Reorganization SOD is the very little biomacromolecule of toxicity and side effect, but as antigen, repeatedly injects in the body and still can bring out immunoreation, so optimal method is the SOD with sod gene clone and expressing human; Perhaps nanorize makes the macromole peptide become little peptide, is beneficial to absorption of human body, and this all is our settled problem.
Traditional Chinese herbal medicine is medical to be single less, mostly be compound recipe, being particular about monarch, is the main treatment disease with one, two flavor monarchs drug often, but because Chinese herbal medicine composition complexity, except that active ingredient, also have a large amount of impurity, even harmful material, have side effects, so that poison, need other ministerial drug assistants to make and eliminate the effects of the act, help to absorb, thereby the prescription volume is excessive, unfavorable use, also existence disappears, and this answers that toxic and side effects, and cancels out each other, and reduces drug effect, be difficult to bring into play the real effect of principal agent, particularly absorption is slow, drug effect is low.
Summary of the invention
The present invention is directed to the prior art deficiency, utilize modern biochemical technology and Chinese medicine and pharmacy theory, the composition and method of making the same of a kind of nanometer SOD and Radix Ginseng or its extract is provided, it is the compositions use in conjunction of nanometer SOD and Radix Ginseng or its extract nanoization, improve drug level and active ingredient, antioxidant activity improves more than 10 times, and skin, mucosa absorption rate reach more than 98%, and the half-life prolongs two more than the order of magnitude; Can enter and directly remove free radical in the cell, have the effect of stronger anti peroxidation of lipid, the comprehensive drug performance is more thorough, it is comparable to be far from traditional herbal mixture, is used to improve anti-anoxia ability, the performance anti-fatigue effect, improve exercise tolerance, be fit to the athlete, emergency case patient, wound wound personnel use, infiltration rate is exceedingly fast, drug effect improves 3-5 doubly than SOD, improves 5-10 doubly than Radix Ginseng, is the contribution greatly to human medicinal career.
The compositions of nanometer SOD and Radix Ginseng or its extract, its proportioning is: nanometer SOD 1-10 ten thousand active units: nano ginseng or its extract 1-50 gram, particle diameter≤10-80 nanometer.
The preparation method of composition of nanometer SOD and Radix Ginseng or its extract is nanorize after the combination of compositions of SOD and Radix Ginseng or its extract; The perhaps combination of nanometer SOD and nano ginseng or its extract.The compositions of described nanometer SOD and Radix Ginseng or its extract is above-mentioned two kinds of compositionss.The nanometer SOD Radix Ginseng, first monomer nanorize recombinant; Perhaps nanorize again after the combination, effect is suitable.
SOD can be the SOD composition of plant or animal extraction.
Radix Ginseng or extract can be compositions such as the ginsenoside, ginseng polysaccharide, ginseng polypeptide, Radix Ginseng hemiterpene of Chinese herbal medicine Radix Ginseng: root contains ginsenoside I～VI etc., divide 2 classes according to its glycoside unit, panaquilon unit is the panaxatriol, and the glycoside unit that ginsenoside IV～VI tells is the panoxadiol; Root contains that volatile oil is about 0.05%, and main component is the Panacene, is the special fragrance of Radix Ginseng source; The Radix Ginseng root still contains panax acid (being palmitic acid, stearic acid, oleic acid and linoleic mixture), plant sterol, choline (0.1～0.2%, be the composition that brings high blood pressure down in the Radix Ginseng), each seed amino acid and peptide class, saccharides such as glucose, fructose, maltose, sucrose, several panose, pectin, vitamin B
1, B
2, nicotinic acid, pantothenic acid etc.; Aerial parts contains nonacosane, 1-larane and cupreol; Contain Saponin again, glycol, panaxatriol and oleanolic acid are joined in hydrolysis then stranger; The ginsenoside is the main component of Radix Ginseng, and content is about 4%, can be divided into the olive acids by glycoside unit difference, Protopanaxatriol's class, the protopanoxadiol class, now isolation identification 29 kinds of saponin components; The ginsenoside presses the size order of silica gel thin-layer chromatography Rf value, ascending called after Ro, Ra, Rb
1, Rb
2, Rc, Rd, Re, Rf, Rg
1, Rg
2, Rg
3, Rh etc.Ginsenoside's great majority are white amorphous powder or colourless crystallization, and little sweetness and bitterness of distinguishing the flavor of has hygroscopicity, generally to acid unstable (except the ginsenoside Ro), be hydrolyzable under the weak acid, but can not get real original shape ruscogenin after hydrolysis.The ginsenoside is soluble in water, methanol, ethanol, dissolves in n-butyl alcohol, acetic acid, ethyl acetate, is insoluble to ether, benzene.Have optical activity, be right-hand helicity more.The aqueous solution jolting produces strong foam.
Nanometer is a long measure, 1 nanometer equal 1 meter part per billion.Nanotechnology is the new and high technology that developed recently gets up.It typically refers to handles atom and molecule in 0.1-100 nanometer bulk, and material is studied or material is processed, thus the new product that preparation has specific function.When a kind of material constantly is cut to a certain degree, its particle is little to be nano material to nanometer scale.Nano material tends to produce some new physicochemical properties.Micronizing is the unit operations that material is crushed to the following granularity of 10um, and air-flowing type commonly used is pulverized.On functional food is made, because the use amount of functional component is very little usually, need micronizing to enough tiny, could guarantee its uniform distribution.Nanotechnology is applied to medical technical elements, has overcome an insurmountable difficult problem on some conventional medicament and the technology, provide brand-new technology for promoting medicine to carry in vivo etc.
Method for making Nano, micro emulsifying method, liposome method and supersonic jet method, it is the new and high technology that contemporary physical chemistry technology can be adopted in biology, medicine and pharmacology field, it is certain fat-soluble that the nanometer SOD Radix Ginseng is had, therefore can protect the biomembrane lipid to avoid radical damage, have stronger lipoid peroxidization resistant; Because granularity is little, has overcome proteinic antigenicity problem, has solved the biomembrane permeability and improved and avoided the destructive stability of protease.
The nanometer SOD Radix Ginseng is a kind of spherical emulsion, and according to inner phase and foreign minister's character, emulsion is divided into two types: a class is that oil content is dispersed in the water, claims oil-in-water emulsions, represents with O/W; Another kind of is in the aqueous dispersion oil, is called for short water in oil type emulsion, represents with W/O.Emulsifying agent is also corresponding so is divided into two types, i.e. water-in-oil emulsifier and oil-in-water emulsifiers.Whether emulsifying agent dissolves easily in water, and just hydrophilic size is an important index for the rational Application emulsifying agent.Because emulsifying agent contains hydrophilic group and lipophilic group simultaneously, interact again between these two bases, for the hydrophilic that compares emulsifying agent just represents with the HLB value that this is a very important empirical method.The HLB value is exactly a hydrophile-lipophile balance value, must select suitable emulsifying agent in order to obtain stable emulsion.The implication of the HLB value of emulsifying agent is: the emulsifying agent hydrophilic percentage ratio HLB value of HLB value is greatly between 0-20.As the HLB value is 0, represents that then hydrophilic is 0, and the HLB value is that 20 expression hydrophilic are 100%.HLB is big more, and the effect of expression hydrophilic is big more; The more little expression lipophile of its value is big more.The nanometer SOD Radix Ginseng also can be a dry powder.Nanometer SOD Radix Ginseng particle diameter can be controlled in≤the 10-80 nanometer range in, further refinement or alligatoring in case of necessity.
The nanometer SOD Radix Ginseng is the free radical street cleaner, remove patient's unnecessary free radical (patient often free radical is more than the normal person), be absorbed by the body when advancing into drug effect at medicine, interior free yl removed, medicine is absorbed by human body comprehensively with nanometer SOD, the performance maximum efficacy, because the drug effect of radical pair medicine directly influences, drug effect was had a greatly reduced quality, the reason of medicine can not bring into play maximum effect that Here it is in the past, have to increase dose, tackle the infringement of free radical on the one hand; Performance is renderd a service to disease on the other hand, has not nowadays had the side effect of free radical, and the efficacy of a drug has just strengthened greatly.Take the nanometer SOD Radix Ginseng for a long time and both can remove free radical, free radical is disappeared or minimizing, its amount of medication has significantly reduced when curing the disease, drug effect is better than compound medicines, and this is the preferred approach that the medicine performance is renderd a service, and is a kind of brand new ideas, will make major contribution for the human health cause, these of the present invention just one big characteristics will cause the revolution of materia medica, biology, pharmacology, pharmacodynamics, cause the new thinking of treatment disease.
Nanometer SOD and Radix Ginseng merge the Therapeutic Method that uses, and utilize both complementary functions, can change both and use the best inadequately deficiency of curative effect respectively.
The use of Chinese medicine is more and more general in the world in recent years, and Chinese medicine and natural goods comprise that the sales volume of antioxidant all increases like multiple every year; We propose to replace or assist western medicine with the Chinese medicine natural goods, have obtained good effect, at home, Thailand, Japan, the U.S. and Europe more and more generally paid attention to.
Confirm by experiment:
1, antioxidant activity experiment: detection method chemiluminescence, protein electrophoresis, biochemical measurement, UV scanning.The tolerant weight of nanometer SOD Radix Ginseng in the capsule: 0.35 ± 0.05g/ grain; Antioxidant activity, every capsules anti-oxidant vigor is equivalent to 32000 unit of activity; Sample 60 ℃ the heating 2 hours after its anti-oxidant vigor be equivalent to 6500 unit of activity; Determining the protein quantity: sample is measured total protein meeting content 9.5mg/ grain, albumin content＜2mg/ grain on Hitachi's automatic clinical chemistry analyzer; Protein electrophoresis is analyzed: sample thief (concentration is 50mg/ml) and bovine serum albumin (25mg/ml), and electrophoresis under the standard conditions, show sample does not have protein band as a result, and the colour developing of standard quality controlled serum and bovine serum albumin is all very clear; Metal ion is measured in the sample: show that every capsules contains magnesium 0.25mg, do not measure calcium, phosphorus, ferrum plasma; UV scanning: it is all constant at the 260nm place to show whether no matter sample heat its maximum absorbance, brief summary: contain anti-oxidation active substance in this sample, improve 10 times than SOD Radix Ginseng.
2, radioprotective experiment: matched group NS; Test group corn nanometer SOD Radix Ginseng, the experiment of peripheral blood leucocyte counting, date processing and result judge, leukocyte count is a measurement data, adopt variance analysis, but need to carry out homogeneity test of variance earlier by the program of variance analysis, variance is neat, calculates the P value, P value＜0.05, conclusion: difference has significance between test group and the matched group, the peripheral blood leucocyte counting of P＜0.05 pre-irradiation is used for respectively organizing the harmonious check of leukocyte count purpose, and dosage group and radiation model control group are relatively, difference does not have significance, proceeds irradiation and subsequent experimental.The leukocyte count that shines back 3 days radiation model control group carries out self relatively with pre-irradiation respectively, and difference has significance, and the radiation damage model is set up, the 5th day, the 14th day dosage group in irradiation back and the comparison of radiation model control group, total white blood cells increases, and difference has significance, and this tests positive.
Leukocyte count is relatively before and after the radiation:
| C(0) | C(5) | C(14) | T(0) | Y(5) | T(14) | |
| M nanometer SOD Radix Ginseng P | 7.55 1.58 | 0.6883 1.31 | 2.2000 0.18 | 7.28 0.48 0.80 | 1.38 1.37 0.0044 | 2.84 0.53 0.0479 |
3, resisting fatigue experiment: medicine preparation (0.5ml//time), experiment group B, 12mg/ml P+L (2/3-100ml); Test group C, 12mg/ml P+L (2/3-100ml); Corn nanometer SOD Radix Ginseng (40000U/ml); Test group D16mg/ml Trionyx sinensis Wiegmann glue; Test group E80mg/ml Trionyx sinensis Wiegmann glue, the alleviating physical fatigue function.Can stablize 24h under the carbamide room temperature in the serum, can stablize more than 7 days at 4-6 ℃.Blood lactic acid is compared with matched group, and experiment group B, C, D, E and control group A compare, and the P value is respectively 0.04,0.84,0.17,0.19.Difference has significance, and this tests positive.
Carbamide in the serum compares:
| A | B | C | D | E |
| UN 9.2±0.9 P | 8.5±1.3 0.2467 | 7.7±1.0 0.0083 ☆ | 9.4±0.9 0.6052 | 8.5±1.2 0.2135 |
Mice swimming with a load attached to the body time ratio is:
| A | B | C | D | E | |
| MN nanometer SOD Radix Ginseng p | 88 20.74825 | 114 31.48714 0.055799 | 116 28.2101 0.028482 | 95 26.96368 0.516779 | 103 13.62351 0.084634 |
4, nanometer SOD Radix Ginseng absorption experiment:
(1), gastric acid toleration result shows that the nanometer SOD Radix Ginseng is 37 ℃ of maintenance 150min in mice simulation gastric acid, vigor still remaining 91%.
(2), Gl tract protease and pancreatin show that to the enzymolysis result of nanometer SOD Radix Ginseng its vigor of maintenance 300min is remaining under 37 ℃ is respectively 92% and 94%.
(3), the amount of appearing of nanometer SOD Radix Ginseng changes over time after the rat small intestine in vitro ligation, the gross activity that adds small intestinal when the gross activity of the outer nanometer SOD Radix Ginseng of small intestinal is near test behind the 10h, this shows that the nanometer SOD Radix Ginseng can see through small intestinal and arrive opposite side from goldbeater's skin one side.
(4), 2h behind its mouse oral absorption nanometer SOD Radix Ginseng, nanometer SOD Radix Ginseng activity reaches peak in the blood, improve 40%%～50% with nanometer SOD in matched group is compared body is active, so oral administration nanometer SOD Radix Ginseng can obviously improve the nanometer SOD Radix Ginseng level in the blood.
(5), the test with Fluorescein isothiocyanate (PITC) marking nano SOD Radix Ginseng shows that label obviously increases in the oral 1h bleeding from anus of mice, reaches summit behind the 2h.
Result of the test confirms that the nanometer SOD Radix Ginseng of oral uptake can enter in the body.Give mouse stomach with the nanometer SOD Radix Ginseng, in blood, reach summit behind the 2h, most of nanometer SOD Radix Ginseng is distributed in the blood plasma, and fraction enters in the hemocyte, and the distribution sequence of nanometer SOD Radix Ginseng in tissue is stomach, intestinal, muscle, lung, brain, liver, brain, kidney, spleen, the heart and testis.
5, nanometer SOD Radix Ginseng resisting fatigue experiment:
Mice swimming with a load attached to the body test
Group dosage number of animals swimming time P value
(g/kg) (only) X ± S
Negative control group 0 10 88 ± 20.42
SOD Radix Ginseng group 80 10 103 ± 14 0.05
Nanometer SOD Radix Ginseng group 80 10 148 ± 32 0.001
Experimental result shows, is tried SOD Radix Ginseng and nanometer SOD Radix Ginseng group mice swimming time and negative control group comparison in the thing, and the SOD Radix Ginseng obviously prolongs (P＜0.05), and the average swimming time of nanometer SOD Radix Ginseng group is significant prolongation (P＜0.001) then.Mice swimming with a load attached to the body result of the test is positive.Two kinds of SOD Radix Ginsengs all have anti-fatigue effect, and the antifatigue effect of nanometer SOD Radix Ginseng is more obvious.
According to the cross correlation of nitric oxide, superoxide radical and superoxide dismutase, the nanometer SOD Radix Ginseng can be used as one of effective ingredient of oral formulations.It can be used for preventing and treating gastral chronic inflammatory disease, and can prevent or the interior cancer of auxiliary treatment digestive tract.High-new engineering enzyme engineering is to utilize the catalytic action of enzyme to carry out the material transformed technology, be that the enzyme that will have specific catalysis in the organism separates, in conjunction with the chemical industry technology, in liquid medium, be fixed on the specific solid phase carrier, as urging the not reactor of biochemical reaction, and enzyme is carried out chemical modification, or adopt the structural transformation of polypeptide chain, enzymology stability, catalytic performance even antigen performance are changed, to reach the engineering of specific purpose.Enzyme engineering is widely used functional food, such as the manufacturing of effect compositions such as functional oligose, peptide, aminoacid, vitamin.Nanometer SOD is a kind of important enzyme of defence oxidative damage in the organism, and energy catalysis superoxide radical generation dismutation reaction is kept the interior ultra-large type oxygen-derived free radicals of cell and is in harmless low-level state.
We use free radical biology, free radical medical science, Chinese medicine are learned and the threpsology, in Radix Ginseng or its extract, add and contain the antioxidant nanometer SOD and antioxidant vitamins is main complexing agent, become the compositions of nanometer SOD Radix Ginseng or its extract, through a series of experiments, prove that it is a kind of medicine efficiently or health product.The permeability of nanometer SOD Radix Ginseng in the human small intestine greatly improves; Rate of release improves; Effect and effective rate of utilization improve greatly; can effectively break through of the restriction of body biological barrier to the effect of medicine food; make medicine food enter blood circulation better; the natural biological barrier that human body has many protection bodies to be without prejudice, as blood brain barrier, blood-eye barrier; cell biological envelope barrier etc.; general medicine is difficult to by these body biological barriers, and nanometer SOD Radix Ginseng microgranule then can pass these barriers, and lesions position is played therapeutical effect.
The nanometer SOD Radix Ginseng, utilize modern biochemical technology and modern Chinese medicine scientific principle opinion, it is the nanorize applied in any combination of the SOD and the Radix Ginseng extract of purification, but not the direct application of SOD and original Chinese herbal medicine Radix Ginseng, improve drug level and active ingredient, antioxidant activity improves more than 10 times, and skin, mucosa absorption rate reach more than 98%, and the half-life prolongs two more than the order of magnitude; Can enter directly to bring into play in the cell and remove free radical, effect with stronger anti peroxidation of lipid, the comprehensive drug performance is more thorough, and it is comparable to be far from Chinese medicine, be used to improve respiratory function, improve anti-anoxia ability, the performance anti-fatigue effect improves exercise tolerance, this technology has overcome the application limitations and the deficiency of bio proteases such as present SOD and Chinese herbal medicine, having opened new gate for its extensive use, is the big invention to human medicinal career, is suitable for penetration and promotion.
Description of drawings
Accompanying drawing 1 of the present invention is that nanometer SOD combines sketch map with Radix Ginseng, and accompanying drawing 2 is a resisting fatigue experimental result sketch map.
The specific embodiment:
Embodiment 1
Nanometer SOD and Radix Ginseng or its extractive composition, proportioning is: nanometer SOD 10,000 active units: nano ginseng 1 gram, particle diameter 50 nanometers.
Embodiment 2
We adopt a kind of corn superoxide dismutase from corn SOD compound enzyme method for large scale production, and the SOD nanorize of producing, nanometer SOD mother solution can reach 6000-10000 active unit/gram; Nanometer SOD dry powder can reach 40000-60000 active unit/gram.
Embodiment 3
Be used for the treatment of the cardiovascular system diseases medicine: nanometer SOD mother solution 6 grams, 60000 active units, nano ginseng 6 grams.
Embodiment 4
Be used to bring into play anti-fatigue effect, improve the exercise tolerance medicine: nanometer SOD dry powder 3 grams, 48000 active units, nano ginseng Saponin 1.5 grams.
Embodiment 5
Be to mix homogenizing with the Radix Ginseng emulsion by weight 2: 1, place nanometer ram machine, pressure 200Mpa, nanochannel 10nm nanometer with purification SOD.
Embodiment 6
Be to mix by the mixed liquid of proportioning and glycerol or other oil-based liquid and surfactant with nanometer SOD and nano ginseng polypeptide, proportioning is 1-10: 1-10: 1-5; After forming emulsion, to wherein dripping surfactant (as n-butyl alcohol), system becomes Water-In-Oil (W/O) microemulsion of clear when being added drop-wise to some.
Embodiment 7
Be that nanometer SOD and Radix Ginseng or its extractive composition as intermediate, are used for the carrier of food, health product, medicine, cosmetics, daily-use chemical industry goods, tobacco and wine goods.
Embodiment 8
Be nanometer SOD and Radix Ginseng or its extractive composition, as the carrier of soft capsule, capsule, Emulsion, tablet, injection or other dosage form.
Embodiment 9
Be nanometer SOD and Radix Ginseng or its extractive composition, can add various medicines according to various objectives, as the carrier of multiple formulations.
Embodiment 10
4-6/2 next day of nanometer SOD Radix Ginseng oral capsule, every day the nanometer SOD Radix Ginseng oral should be greater than 1.2 ten thousand active units; The treatment wound can 50,000 active units; In treatment sexual impotence 4-15g nanometer SOD 10,000 active units; Be suitable for aged debility, safety good (Radix Pseudostellariae, Radix Codonopsis, Radix Panacis Quinquefolii), consumption reduces after adding nanometer SOD, reduce the nasal bleeding side effect, Radix Ginseng is in the course of processing, many organized enzymes are killed, and therefore add the effect that SOD can strengthen its defying age, radioprotective, increase intelligence and treatment cardiovascular disease.
Claims (10)
1, the compositions of nanometer SOD and Radix Ginseng or its extract is that proportioning is: nanometer SOD 1-10 ten thousand active units: nano ginseng or its extract 1-50 gram, particle diameter≤80 nanometers.
2, according to the compositions of the described nanometer SOD of claim 1 and Radix Ginseng or its extract, be that proportioning is: nanometer SOD 10,000 active units: nano ginseng 1 gram, particle diameter 50 nanometers.
3,, be that extract is ginsenoside, ginseng polysaccharide, ginseng polypeptide, Radix Ginseng hemiterpene according to the compositions of the described nanometer SOD of claim 1 and Radix Ginseng or its extract.
4, according to the compositions of the described nanometer SOD of claim 1 and Radix Ginseng or its extract, be to be used for the treatment of the cardiovascular system diseases medicine: nanometer SOD mother solution 6 grams, 60000 active units, nano ginseng 6 grams.
5, according to the compositions of the described nanometer SOD of claim 1 and Radix Ginseng or its extract, be to be used to bring into play anti-fatigue effect, improve the exercise tolerance medicine: nanometer SOD dry powder 3 grams, 48000 active units, nano ginseng Saponin 1.5 grams.
6, the preparation method of composition of nanometer SOD and Radix Ginseng or its extract is nanorize after the combination of compositions of SOD and Radix Ginseng or its extract; The perhaps combination of nanometer SOD and nano ginseng or its extract.
7,, be that the nanometer SOD mother solution reaches 6000-10000 active unit/gram according to the preparation of compositions method of the described nanometer SOD of claim 6 and Radix Ginseng or its extract; Nanometer SOD dry powder reaches 40000-60000 active unit/gram.
8, according to the preparation of compositions method of the described nanometer SOD of claim 6 and Radix Ginseng or its extract, be to mix homogenizing with the Radix Ginseng emulsion by weight 2: 1 with purification SOD, place nanometer ram machine, pressure 200Mpa, nanochannel 10nm.
9, according to the preparation of compositions method of the described nanometer SOD of claim 6 and Radix Ginseng or its extract, be to mix by the mixed liquid of proportioning and glycerol or other oil-based liquid and surfactant with nanometer SOD and nano ginseng polypeptide, proportioning is 1-10: 1-10: 1-5; After forming emulsion, to wherein dripping surfactant, system becomes Water-In-Oil (W/O) microemulsion of clear when being added drop-wise to some.
10, the compositions of nanometer SOD and Radix Ginseng or its extract is used to prepare the carrier of food, health product, medicine, cosmetics, daily-use chemical industry goods, tobacco and wine goods as intermediate.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102125572A (en) * | 2010-12-30 | 2011-07-20 | 黑龙江珍宝岛药业股份有限公司 | Pharmaceutical composition and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102217757B (en) * | 2011-04-27 | 2015-05-20 | 张卫国 | Plant superoxide dismutase buccal tablets and preparation method thereof |
| CN102250740B (en) * | 2011-07-25 | 2012-09-05 | 周昭明 | Ginseng superoxide dismutase (SOD) red wine and preparation method thereof |
| CN102309751B (en) * | 2011-08-31 | 2012-12-26 | 苏州爱斯欧蒂生物科技有限公司 | Superoxide dismutase (SOD) composite capsule and preparation method thereof |
| CN114796120B (en) * | 2022-05-25 | 2024-04-02 | 中山大学 | Anti-hair loss hair-growing liposome and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1110178A (en) * | 1994-04-08 | 1995-10-18 | 上海中药制药二厂 | Superoxide dismutase capsule and preparation method thereof |
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| CN1110178A (en) * | 1994-04-08 | 1995-10-18 | 上海中药制药二厂 | Superoxide dismutase capsule and preparation method thereof |
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| CN102125572A (en) * | 2010-12-30 | 2011-07-20 | 黑龙江珍宝岛药业股份有限公司 | Pharmaceutical composition and application thereof |
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