CN1742763A - Use of Wucenglong extract in preparing health-care product and medicines - Google Patents
Use of Wucenglong extract in preparing health-care product and medicines Download PDFInfo
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- CN1742763A CN1742763A CNA200510081547XA CN200510081547A CN1742763A CN 1742763 A CN1742763 A CN 1742763A CN A200510081547X A CNA200510081547X A CN A200510081547XA CN 200510081547 A CN200510081547 A CN 200510081547A CN 1742763 A CN1742763 A CN 1742763A
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Abstract
The present invention discloses a method for preparing extract of salacia prinoides and its new application. Its preparation method includes the following steps: drying, pulverizing, extracting, centrifugalization, concentrating and drying. Said extract has the action of reducing blood sugar, can be used for preparing health-care food and medicine for preventing and curing diabetes.
Description
Technical field
The present invention relates to the preparation method of the Radix salaciae prinoidis extract and the purposes of extract, relate in particular to the preparation method and the purposes of this extract in preparation health product and medicine of the Radix salaciae prinoidis extract.
Background technology
Salacia prinoides (Willd.) DC. belongs to Hippocrateaceae (Hippocrateaceae) plant, and for climbing or upright shrub, its root can be used as medicine, and has dredge the meridian passage, and the effect of expelling wind and removing dampness is mainly used in the treatment of rheumatic arthralgia, lumbar muscle strain, body nihility power clinically.
In recent years, the sickness rate of diabetes is the trend that increases year by year, has become the commonly encountered diseases and the frequently-occurring disease of the many countries in the whole world, and being needs top-priority disease after many countries are only second to cancer, acquired immune deficiency syndrome (AIDS) and cardiovascular disease.The patient is because blood glucose can not get effective control, and causes and lead to complications the irreversible pathological changes of organs such as the heart, kidney, brain, eye.Because its cause of disease and pathogenesis are still not fully aware of, can only utilize as early as possible at present various hypoglycemic drug blood sugar control rising, reduce insulin resistant etc., with the development of the control course of disease, postpone or reduce the generation of complication.Common oral hypoglycemic has four classes: insulin secretagogue agent, euglycemic agent, alpha-glucosidase inhibitor and compound Chinese medicinal preparation, but there are the few characteristics of action target spot in chemical drugs to treatment of diabetes.Along with people to the further investigation of onset diabetes mechanism and the proposition of " metabolism syndrome ", with glucose level control, improve insulin resistant and have a kind of new trend that many target treatments of preventing and treating complication are current diabetes controls concurrently.Though the blood sugar lowering speed of Chinese herbal medicine and intensity are not as chemical drugs, effect relaxes, few side effects, and the multiformity of Chinese herbal medicine active component shows that it has bigger advantage on many target treatments of diabetes simultaneously.
Summary of the invention
The preparation method and the new purposes of this extract in preparation health product and medicine that the purpose of this invention is to provide the Radix salaciae prinoidis extract.
The preparation method of the Radix salaciae prinoidis extract that the present invention is designed is: after Salacia prinoides (Willd.) DC. drying, pulverizing, add solvent and extract, then that feed liquid is centrifugal, merging, obtain extracting solution, extracting solution is concentrated, spray drying get the Radix salaciae prinoidis extract (I), through sterilization, sealing, preserve standby.Also can be with gained extracting solution partial concentration, behind the thin up, use nonpolar or the absorption of low pole macroporous adsorptive resins, use solvent elution then, after the eluent reconcentration was removed partial solvent, spray-dried or vacuum drying or airpillow-dry got powder and are the Radix salaciae prinoidis extract (II), through sterilization, sealing, preserve standby.Described Salacia prinoides (Willd.) DC. is meant one or both and the two or more mixing in broad-leaved Salacia prinoides (Willd.) DC. (Salacia amplifolia), Hainan Salacia prinoides (Willd.) DC. (Salacia hainanensis), stockless Salacia prinoides (Willd.) DC. (Salacia sessiliflora), the close colored Salacia prinoides (Willd.) DC. (Salaciaconfertiflora); Described solvent is a kind of in water, methanol, ethanol, the acetone, or two or more mixed solvent of forming with any ratio.
The invention still further relates to the blood sugar lowering effect that utilizes the Radix salaciae prinoidis extract to have, preparation can be treated or the health product or the medicine of prevent diabetes.
In order to understand the purposes of the Radix salaciae prinoidis extract of the present invention better, pharmacological evaluation and the result with the broad-leaved Radix salaciae prinoidis extract illustrates its new purposes in health care and pharmaceutical field below.Similarly, other Radix salaciae prinoidis extract also can reach same pharmacological effect, and difference only is the difference on the use amount.
(1) observes the influence of extract to the hyperglycemia model mouse blood sugar level of model induced by alloxan
Get normal mouse tail vein injection alloxan (5% aqueous solution, 60mg/kg), behind the 72h, the mice behind the fasting 8h (can't help water) is carried out the eye socket venous blood collection, measure serum level of glucose, select blood glucose value to try animal as confession the mice of 9.99mmol/L.To be for examination mice random packet: alloxan group (model group), metformin group (100mg/kg), broad-leaved Radix salaciae prinoidis extract group (I) big (4g/kg), in (2g/kg), little (1g/kg) three dosage groups, broad-leaved Radix salaciae prinoidis extract group (II) big (2g/kg), little (0.5g/kg) two dosage groups, every group 10, other gets with the normal mouse of quantity and makes the blank group.Every day, gastric infusion was 1 time, and blank group and model group give isopyknic distilled water respectively, continuously 10d.1h after the last administration gets blood from the mouse orbit vein, uses the kit measurement blood glucose value.
The table 1 broad-leaved Radix salaciae prinoidis extract is to the influence of the hyperglycemia mouse blood sugar level of model induced by alloxan (x ± S)
Group | Number of animals (only) | Dosage (g/kg) | Blood glucose (mmol/L) | |
Before the administration | After the administration | |||
The blank group | 10 | 4.96±1.05 | 4.89±1.06 | |
Model group Model | 10 | 19.87±2.56 △△ | 19.45±2.60 △△ | |
Broad-leaved Radix salaciae prinoidis extract group (I) | 10 | 1 | 19.74±2.36 | 16.60±2.76 * |
Broad-leaved Radix salaciae prinoidis extract group (I) | 10 | 2 | 20.01±2.45 | 15.23±2.48 ** |
Broad-leaved Radix salaciae prinoidis extract group (I) | 10 | 4 | 19.82±1.79 | 13.05±1.89 **# |
Broad-leaved Radix salaciae prinoidis extract group (II) | 10 | 0.5 | 19.88±1.89 | 16.54±2.65 * |
Broad-leaved Radix salaciae prinoidis extract group (II) | 10 | 2 | 19.76±1.46 | 12.45±1.78 **# |
The metformin group | 10 | 0.1 | 19.84±2.76 | 15.36±2.54 ** |
Annotate: △ represents to compare with the blank group,
△ △P<0.01; * expression is compared with model group,
*P<0.05,
*P<0.01; # represents to compare with the metformin group,
#P<0.05.
(2) observe extract glucose is caused the influence that mouse blood sugar raises
Get 60 of normal mouses, random packet is: blank group, glucose group, metformin group (100mg/kg), broad-leaved Radix salaciae prinoidis extract group (I) big (4g/kg), in (2g/kg), little (1g/kg) three dosage groups, broad-leaved Radix salaciae prinoidis extract group (II) big (2g/kg), little (0.5g/kg) two dosage groups, 10 every group.Every day, gastric infusion was 1 time, and blank group and glucose group give isopyknic distilled water respectively, continuously 10d.1h after the last administration, blank group lumbar injection equal-volume normal saline, all the other respectively organize lumbar injection glucose (2g/kg) solution, respectively behind the lumbar injection glucose solution 0.5,1.0, behind the 2.0h, get blood from the mouse orbit vein, use the kit measurement blood glucose value.
The influence of the hyperglycemia model mouse blood sugar level that the table 2 broad-leaved Radix salaciae prinoidis extract causes glucose (x ± S)
Group | Number of animals (only) | Dosage (g/kg) | Blood glucose (mmol/L) | ||
0.5h | 1.0h | 2.0h | |||
Blank group | 10 | 6.36±1.05 | 6.52±1.27 | 5.04±1.16 | |
Glucose group | 10 | 14.27±2.21 △△ | 14.56±1.98 △△ | 14.05±2.43 △△ | |
Broad-leaved Radix salaciae prinoidis extract group (I) | 10 | 1 | 9.34±1.78 ** | 10.16±1.65 ** | 12.36±2.56 |
Broad-leaved Radix salaciae prinoidis extract group (I) | 10 | 2 | 8.78±2.16 ** | 9.97±1.95 ** | 11.91±2.18 |
Broad-leaved Radix salaciae prinoidis extract group (I) | 10 | 4 | 7.92±1.69 ** | 8.56±1.75 ** | 10.04±1.89 ** |
Broad-leaved Radix salaciae prinoidis extract group (II) | 10 | 0.5 | 8.91±2.06 ** | 9.96±2.10 ** | 11.96±2.32 |
Broad-leaved Radix salaciae prinoidis extract group (II) | 10 | 2 | 7.65±1.79 ** | 8.35±1.85 ** | 9.92±1.74 ** |
The metformin group | 10 | 0.1 | 8.06±1.61 ** | 8.71±1.67 ** | 9.15±1.64 ** |
Annotate: △ represents to compare with blank group,
△ △P<0.01; * expression is compared with model group,
*P<0.01.
Above experimental result is learned processing by statistics, show broad-leaved Salacia prinoides (Willd.) DC. root extract (I) and (II) under test dose, all can significantly reduce the blood sugar level of the hyperglycemia model mice of model induced by alloxan, and heavy dose of group obviously is better than small dose group; Broad-leaved Salacia prinoides (Willd.) DC. root extract (I) and test dose group (II) cause that to exogenous glucose the rising of mouse blood sugar also has the obvious suppression effect in 1h simultaneously, and heavy dose of group is particularly evident.
Above result can draw and the invention has the advantages that:
The invention provides the Radix salaciae prinoidis extract and have significant blood sugar lowering effect, can be used to prepare the health food and the medicine of prevention and treatment diabetes.
The preparation method technology of the Radix salaciae prinoidis extract provided by the present invention is simple, and cost is low, can effectively the extracts active ingredients in the Salacia prinoides (Willd.) DC. be come out, and applies to prepare health product or medicine.
The specific embodiment
The present invention is further described with indefiniteness embodiment below.
Example 1.
Take by weighing the coarse powder 10kg after the broad-leaved Salacia prinoides (Willd.) DC. plant roots drying and crushing, the ethanol extraction with 50%, extracting solution merges, concentrating under reduced pressure, spray drying gets the 0.8kg broad-leaved Radix salaciae prinoidis extract, gets this extract 200g, add micropowder silica gel 150g, magnesium stearate 40g, behind the mix homogeneously, add entry 30mL moistening, add Polyethylene Glycol (4000) 20g again as binding agent, by known granulation technique, make every capsule that contains the 150mg extract.
Example 2.
Take by weighing the coarse powder 5.0kg after the Salacia prinoides (Willd.) DC. plant roots drying and crushing of Hainan, extracting in water, extracting solution merges, concentrating under reduced pressure, spray drying gets 604g Hainan Radix salaciae prinoidis extract, gets this extract 200g, add micropowder silica gel 120g, magnesium stearate 50g, behind the mix homogeneously, add a small amount of 95% ethanol moistening after, add Polyethylene Glycol (4000) 25g again as binding agent, by known granulation technique, make every capsule that contains the 100mg extract.
Example 3.
Take by weighing the coarse powder 8.0kg after the stockless Salacia prinoides (Willd.) DC. plant roots drying and crushing, add 95% ethanol extraction, extracting solution merges, concentrating under reduced pressure, spray drying gets the 534g stockless Radix salaciae prinoidis extract, gets this extract 120g, add micropowder silica gel 120g, magnesium stearate 50g, behind the mix homogeneously, add a small amount of 95% ethanol moistening after, add Polyethylene Glycol (4000) 15g again as binding agent, by known granulation technique, make every capsule that contains the 100mg extract.
Example 4.
Take by weighing the coarse powder 4.0kg after the broad-leaved Salacia prinoides (Willd.) DC. plant roots drying and crushing, add 30% ethanol extraction, extracting solution merges, concentrating under reduced pressure, spray drying gets the 430g broad-leaved Radix salaciae prinoidis extract, gets this extract 100g, add micropowder silica gel 150g, magnesium stearate 30g, behind the mix homogeneously, add a small amount of 95% ethanol moistening after, add Polyethylene Glycol (4000) 15g again as binding agent, by known granulation technique, make every capsule that contains the 150mg extract.
Example 5.
Take by weighing the coarse powder 6.0kg after the Salacia prinoides (Willd.) DC. plant roots drying and crushing of Hainan, add 40% ethanol extraction, extracting solution merges, the decompression part is concentrated into proportion 1.25, adds suitable quantity of water and disperses suspendible, with D101 macroporous adsorbent resin on the suspension, water successively, 70% ethanol elution, 75% ethanol elution, behind the concentrating under reduced pressure, spray drying, get the 238g powder, get this powder 50g, add micropowder silica gel 100g, magnesium stearate 20g is behind the mix homogeneously, after adding a small amount of 95% ethanol moistening, add Polyethylene Glycol (4000) 10g again as binding agent,, make every capsule that contains the above-mentioned powder of 80mg by known granulation technique.
Example 6.
Take by weighing the coarse powder 8.0kg after the broad-leaved Salacia prinoides (Willd.) DC. plant roots drying and crushing, extracting in water, extracting solution merges, the decompression part is concentrated into proportion 1.10, after the cooling, with AB-8 macroporous adsorbent resin on the concentrated solution, water successively, 40% ethanol elution, 40% ethanol elution, behind the concentrating under reduced pressure, spray drying, get the 176g powder, get this powder 100g, add micropowder silica gel 150g, magnesium stearate 100g is behind the mix homogeneously, after adding a small amount of 95% ethanol moistening, add Polyethylene Glycol (6000) 50g again as binding agent,, make every capsule that contains the above-mentioned powder of 60mg by known granulation technique.
Claims (5)
1, a kind of preparation method of the Radix salaciae prinoidis extract is characterized in that: after Salacia prinoides (Willd.) DC. drying, pulverizing, add solvent and extract, then that feed liquid is centrifugal, merging obtain extracting solution, extracting solution is concentrated, spray drying, get the Radix salaciae prinoidis extract (I),, preserve standby through sterilization, sealing.
2, according to the preparation method of the said Radix salaciae prinoidis extract of claim 1, it is characterized in that: can be gained extracting solution partial concentration, behind the thin up, use nonpolar or the absorption of low pole macroporous adsorptive resins, use solvent elution then, after the eluent reconcentration is removed partial solvent, spray-dried or vacuum drying or airpillow-dry, getting powder promptly is the Radix salaciae prinoidis extract (II), through sterilization, sealing, preserves standby.
3, according to the extracting method of the said Radix salaciae prinoidis extract of claim 1, it is characterized in that: described Salacia prinoides (Willd.) DC. is meant one or both and the two or more mixing in broad-leaved Salacia prinoides (Willd.) DC. (Salacia amplifolia), Hainan Salacia prinoides (Willd.) DC. (Salacia hainanensis), stockless Salacia prinoides (Willd.) DC. (Salacia sessiliflora), the close colored Salacia prinoides (Willd.) DC. (Salacia confertiflora).
4, according to the preparation method of the claim 1 or the 2 said Radix salaciae prinoidis extracts, it is characterized in that: described solvent is a kind of in water, methanol, ethanol, the acetone, or two or more mixed solvent of forming with any ratio.
5, the Radix salaciae prinoidis extract that is prepared by the preparation method of the claim 1 or the 2 said Radix salaciae prinoidis extracts is in preparation prevention or the health food of treatment diabetes and the application in the medicine.
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CN101815443B (en) * | 2007-09-04 | 2013-08-21 | 富士胶片株式会社 | Foodstuff of tablets or capsules |
CN101888848B (en) * | 2007-12-07 | 2013-03-27 | 富士胶片株式会社 | Agent for reducing intestinal toxic bacterium and food or pharmaceutical preparation comprising the same |
CN106176883A (en) * | 2009-04-22 | 2016-12-07 | 富士胶片株式会社 | Agent for regulating composition ratio of intestinal bacterial flora |
CN102665760B (en) * | 2010-02-25 | 2014-09-03 | 富士胶片株式会社 | Primary bile acid and secondary bile acid generation regulator |
CN102665760A (en) * | 2010-02-25 | 2012-09-12 | 富士胶片株式会社 | Primary bile acid and secondary bile acid generation regulator |
CN103874426A (en) * | 2011-08-16 | 2014-06-18 | 雅培制药有限公司 | Nutritional compositions comprising a soluble viscous fiber and a polyphenol-containing plant extract |
CN103848825B (en) * | 2012-12-03 | 2017-01-25 | 中国药科大学 | Preparation method and application of sulfonium sugar natural product with inhibition activity on alpha-glucosidase and derivatives thereof |
CN103848825A (en) * | 2012-12-03 | 2014-06-11 | 中国药科大学 | Preparation method and application of sulfonium sugar natural product with inhibition activity on alpha-glucosidase and derivatives thereof |
CN105816487A (en) * | 2015-01-06 | 2016-08-03 | 周亚伟 | Novel application of salacia prinoides |
CN107372947A (en) * | 2017-06-21 | 2017-11-24 | 武汉市元大生物科技有限公司 | One kind bubble pocket type blood-sugar reducing tea and preparation method thereof |
CN108125243A (en) * | 2018-03-09 | 2018-06-08 | 北京素维生物科技有限公司 | A kind of composition and its preparation process for inhibiting energy absorption |
CN109096061A (en) * | 2018-10-08 | 2018-12-28 | 云南民族大学 | A kind of new small molecule phenolic compound salaciacochine A and its preparation method and application |
CN109096061B (en) * | 2018-10-08 | 2021-05-11 | 云南民族大学 | Novel micromolecular aromatic compound salacia cochine A as well as preparation method and application thereof |
CN115153024A (en) * | 2022-07-19 | 2022-10-11 | 中山市嘉信医疗器械有限公司 | Health food for regulating blood sugar and preparation method thereof |
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