CN1286373C - Propolis-silver ion composite nanometer disinfectant and preparation method thereof - Google Patents
Propolis-silver ion composite nanometer disinfectant and preparation method thereof Download PDFInfo
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Abstract
The present invention discloses the nanometer disinfectant compounded by propolis and silver ions, which is oil-in-water type emulsion which comprises the propolis of which the concentration is in a scope ranging from 0.125 to 2g/100 ml and the average particle diameter is in a scope ranging from 30 to 100 nanometers and the silver ions of which the concentration is in a scope ranging from 3.125 to 50 ug/l. The present invention has a preparation method for the nanometer disinfectant compounded by the propolis and silver ions that the propolis is dissolved in organic solvent, emulsifier drops are added in the propolis organic solution under the condition of room temperature, and simultaneously, mixed liquor is magnetically stirred until the mixed liquor is clarifying so as to form an oil phase; then, the mixed liquor is stirred and silver ion water solution is dropped and added in the mixed liquor until composite emulsion is finally formed. The emulsion of the present invention has the characteristics of short sterilization time and obvious extermination effect; the present invention can be safely used on skin of a human body and mucous membrane without harm and stimulation effects on environment and the human body, and can be used for preventing and restraining the infection and the pathogenesis of terror sources; in addition, the present invention can carry out disinfection for contaminated environment, devices for civil use, precise instruments, etc.
Description
Technical field
The present invention relates to a kind of Chemical composition that, be specifically related to a kind of novel propolis and silver ion composite nanometer disinfectant and preparation and application as disinfectant.
Background technology
After the calendar year 2001 U.S. " 9.11 " terrorist incident, in a plurality of countries the mailing of " white powder "-anthrax spore appears in succession, and bio-terrorism becomes a reality, and more causes national governments' showing great attention to bio-terrorism.The development of biotechnology and gene engineering is advanced by leaps and bounds, and not only makes the bio-terrorism cause of disease be more prone to obtain, and rebuild and change structure deadly infectious disease is former, has further strengthened the threat that bio-terrorism attacks and the difficulty of biological anti-terrorism.Can use the disinfectant of the similar bio-terrorism cause of disease of reply to be organic chemical industry's goods such as formaldehyde, hypochlorous acid etc. at present, the toxicity of these disinfectants own is bigger, not only the human body skin mucous membrane is had certain damage, and can cause environmental pollution.Therefore, development is efficient, wide spectrum is killed the bio-terrorism cause of disease, can use safely at human body, effectively prevent and suppress the former infection of bio-terrorism, morbidity, and can become one of important topic that bio-terrorism protects the new disinfectant of the sterilizations such as environment, civilian installation and precision instrument of polluting.
The innovation and creation content
The purpose of this invention is to provide a kind of novel propolis and silver ion composite nanometer disinfectant of can wide spectrum killing the bio-terrorism cause of disease.
Another object of the present invention provides the preparation method of a kind of above-mentioned propolis and silver ion composite nanometer disinfectant.
Propolis provided by the invention and silver ion composite nanometer disinfectant are that to contain concentration be that 0.125～2g/100ml, average grain diameter propolis and the concentration between 30～100 nanometers is the oil-in-water emulsion of the silver ion of 3.125～50 μ g/l.
In above-mentioned propolis and the silver ion composite nanometer disinfectant, also comprise organic solvent, described organic solvent is ethanol, methyl alcohol, a kind of in n-butanol or the acetone; Used emulsifier is a kind of in Tween-20, Tween-60 or the Tween-80 in the described emulsion; Described silver ion comes from silver nitrate or silver orthophosphate.
In above-mentioned propolis and the silver ion composite nanometer disinfectant, in per 100 parts by volume emulsions, contain 2 weight portion propolis, 5 parts by volume ethanol, 10～15 parts by volume Tween-80s, 5 * 10
-6The weight portion silver nitrate, and when parts by volume was milliliter, weight portion corresponded to gram, and the rest may be inferred.
The invention provides the preparation method of a kind of described propolis and silver ion composite nanometer disinfectant, comprise following operation: propolis is dissolved in the organic solvent, under the room temperature condition, emulsifier is added dropwise in this propolis organic solution, carry out magnetic agitation simultaneously, form oil phase until the mixed liquor clarification, drip silver ionized water solution then while stirring and be described disinfectant until final formation multiple emulsion.
Among the preparation method of above-mentioned propolis and silver ion composite nanometer disinfectant, emulsifier is added dropwise to rate of addition is 1～2ml/min in the propolis organic solution; Rate of addition is 3～5ml/min when dripping silver ionized water solution.
Among the preparation method of above-mentioned propolis and silver ion composite nanometer disinfectant, the optimum volume ratio scope of described emulsifier and propolis organic solution is 1: 1～2: 1.The optimum volume ratio scope of the oil phase that water that described silver ionized water solution forms and emulsifier and propolis organic solution form is 7: 3～4: 1.
Adopt technique scheme, the present invention to utilize propolis wide spectrum antibacterium, fungi and virus and nontoxic characteristic, be equipped with silver ion, improve the activity that propolis is killed pathogenic microorganisms, especially to the deactivation of bacillus gemma; Adopt the inventive method, do not use powerful emulsification machinery, only gentle agitation at room temperature has hydrophilic, oleophilic characteristics by utilizing emulsifier, drips silver ionized water solution, multiple emulsion that can spontaneous formation propolis nano-scale particle.Experimental results show that, emulsion of the present invention has that sterilizing time is short, the tangible characteristics of killing effect, toxicity test shows that this emulsion greatly reduces the content of propolis lytic agent moderate stimulation organic solvent, can use safely on human body skin, mucous membrane, and environment and human body are not had harm and spread effect.Thereby make the NEW TYPE OF COMPOSITE nanometer disinfectant of preparation can potently kill multiple pathogenic microorganisms, and can under multiple condition, use safely.
Embodiment
Antibiotic or the antibacterial functional component that adopts among the present invention is propolis and silver ion.
Studies show that, the antibiotic main active of propolis is contained flavonoids of propolis and polyphenol, utilize micro-calorimetry and electron micrograph technical result to show, propolis can be by stoping the cell differentiation bacteria growing inhibiting, by destroying the order state of cell membrane and endochylema, cause inhibition (the Koo H that part bacteriolyze and bacterioprotein are synthetic, Gomes BP, Rosalen PL.In vitro antimicrobial activity of propolis and arnica Montanaagainst oral pathogens.Arch oral Biol.2000; 45 (2): 1416.).
Studies show that, during the silver ion low concentration (as 50 μ g/l),,, but still have certain vitality (bacteriostasis) though bacterium has some physiological function destroyed can brute force is adsorbed in the cell wall surface; Adsorb at cell surface during the silver ion high concentration, excessive silver ion will enter cell interior by the penetration cell wall; Be trapped on the cell serous coat, with the S on the enzyme that contains sulfydryl herein, O, the combination of N atom, the activity of inhibitory enzyme makes bacterium death (bactericidal action) (Yahya MT, StraubTM, Gerba CP etal.Inactivation of coliphage MS-2 and poliovirus by copper, silver, andchlorine.Can J Microbiol.1992; 38 (5): 430.)
The present invention is a kind of disinfectant with propolis and the compound use of silver ion, and makes it to form a kind of oil-in-water emulsion structure.The toxicity of considering disinfectant should be controlled at zone of reasonableness, the highest 50 μ g/l that are limited to of content of the silver ion that the present invention uses, the single silver ion under this concentration only has bacteriostasis, but with the inventive method with itself and the compound use of propolis, then form synergistic effect, have sterilizing function.
The present invention utilizes propolis organic solution and silver ionized water solution to be primary raw material, is equipped with an amount of emulsifier, with suitable mode it is mixed a kind of emulsion of formation and promptly obtains disinfectant of the present invention.In this disinfectant, oil phase comprises propolis, a kind of or several are as being used to dissolve the organic solvent and the emulsifier of propolis, wherein organic solvent can be Different concentrations of alcohol solution or methyl alcohol, n-butanol, acetone etc., emulsifier is Tweens activating agent a kind of of variable concentrations, as Tween-20, and Tween-60, Tween-80 etc., the average grain diameter of propolis is between the 30-100 nanometer; Water is a silver ionized water solution, can be by certain density silver ionized water solution compositions such as silver nitrate, silver orthophosphates.In the disinfectant of the present invention, the propolis final concentration is 0.125～2.0g/100ml more fortunately, and concentration of silver ions 3.125～50 μ g/l more fortunately.
The present invention also provides the preparation method of a kind of propolis and silver ion composite nanometer disinfectant, this method is not used powerful emulsification machinery, gentle agitation at room temperature only, by utilizing emulsifier to have hydrophilic, oleophilic characteristics, drip silver ionized water solution, multiple emulsion that can spontaneous formation propolis nano-scale particle.Concrete steps are: under the room temperature condition, emulsifier is added dropwise in the propolis organic solution, magnetic agitation to mixed liquor clarification forms oil phase; Drip silver ionized water solution then while stirring, be brown yellow transparent liquid, promptly obtain disinfectant of the present invention until final multiple emulsion.In preparation process, wherein the optimum volume ratio scope of emulsifier and propolis organic solution is 1: 1～2: 1 in the oil phase, and the optimum volume ratio scope of water and oil phase is 7: 3～4: 1.
Embodiment 1,
It is among 50% the ethanol 10ml that propolis 2g is dissolved in volumetric concentration, under the room temperature condition, the 10ml Tween-80 is added dropwise in this propolis ethanolic solution, and rate of addition 1～2ml/min carries out magnetic agitation simultaneously, forms oil phase until the mixed liquor clarification; Dripping concentration then while stirring is the silver nitrate aqueous solution 80ml of 62.5 μ g/l, and rate of addition 3～5ml/min is brown yellow transparent liquid until final multiple emulsion, promptly obtains this routine disinfectant 100ml.
Each component final concentration is respectively in this routine disinfectant: propolis 2g/100ml, ethanol 5% (v/v), Tween-80 are 10% (v/v), silver nitrate 5 μ g/100ml.Propolis particle average grain diameter is about 50nm in the emulsion.
Embodiment 2,
Propolis 2g is dissolved among the straight alcohol 5ml, under the room temperature condition, the 15ml Tween-80 is added dropwise in this propolis ethanolic solution, rate of addition 1～2ml/min carries out magnetic agitation simultaneously, forms oil phase until the mixed liquor clarification; Dripping concentration then while stirring is the silver nitrate aqueous solution 80ml of 62.5 μ g/l, and rate of addition 3～5ml/min is brown yellow transparent liquid until final multiple emulsion, promptly obtains this routine disinfectant 100ml.
Each component final concentration is respectively in this routine disinfectant: propolis 2g/100ml, ethanol 5% (v/v), Tween-80 are 15% (v/v), silver nitrate 5 μ g/100ml.Propolis particle average grain diameter is about 90nm in the emulsion.
Embodiment 3,
It is among 50% the n-butanol 10ml that propolis 1g is dissolved in volumetric concentration, under the room temperature condition, the 20ml Tween-60 is added dropwise in this propolis butanol solution, and rate of addition 1～2ml/min carries out magnetic agitation simultaneously, forms oil phase until the mixed liquor clarification; Dripping concentration then while stirring is the silver orthophosphate aqueous solution 70ml of 71.4 μ g/l, and rate of addition 3～5ml/min is brown yellow transparent liquid until final multiple emulsion, promptly obtains this routine disinfectant 100ml.
Each component final concentration is respectively in this routine disinfectant: propolis 1g/100ml, n-butanol 5% (v/v), Tween-60 are 20% (v/v), silver orthophosphate 5 μ g/100ml.Propolis particle average grain diameter is about 60nm in the emulsion.
Embodiment 4,
It is among 50% the ethanol 10ml that propolis 1g is dissolved in volumetric concentration, under the room temperature condition, the 15ml Tween-80 is added dropwise in this propolis ethanolic solution, and rate of addition 1～2ml/min carries out magnetic agitation simultaneously, forms oil phase until the mixed liquor clarification; Dripping concentration then while stirring is the silver nitrate aqueous solution 75ml of 33.3 μ g/l, and rate of addition 3～5ml/min is brown yellow transparent liquid until final multiple emulsion, promptly obtains this routine disinfectant 100ml.
Each component final concentration is respectively in this routine disinfectant: propolis 1g/100ml, ethanol 5% (v/v), Tween-80 are 15% (v/v), silver nitrate 2.5 μ g/100ml.Propolis particle average grain diameter is about 30nm in the emulsion.
After tested, the disinfectant of the foregoing description preparation all satisfies following physicochemical character:
Outward appearance: pale brown look transparent
PH value: 6.5
The centrifugal back of 4500rpm: no layering or muddiness
Particle diameter under the Electronic Speculum (nm): 30-100
General flavone content (g): 0.55
Antibacterial, the bactericidal assay of propolis of the present invention and silver ion composite nanometer disinfectant
1. each component is to the inhibitory action of Escherichia coli 8099 relatively in multiple emulsion and the emulsion
The propolis and the silver ion composite nanometer disinfectant (abbreviation multiple emulsion) of embodiment 1 preparation were made 1: 4 of sterile water, 1: 8,1: 16,1: 32 dilution, carry out the suspension bacteriostatic experiment of Escherichia coli 8099 according to the requirement of disinfection technology standard, determine minimal inhibitory concentration (MIC), and contrast the bacteriostasis of variable concentrations, propolis, Tween-80, ethanol simultaneously.The results are shown in Table 1:
Table 1 multiple emulsion is to the bacteriostasis of Escherichia coli 8099
| Grouping | Bacteriostatic agent | Dilution ratio | To the Escherichia coli fungistatic effect |
| Experimental group | Dilution back multiple emulsion | 1∶4 | - |
| 1∶8 | - | ||
| 1∶16 | - | ||
| 1∶32 | + | ||
| Tween-80 (v/v) | 10％ | + | |
| 5％ | + | ||
| 2.5％ | + |
| Control group | Ethanol (v/v) | 25％ | - |
| 12.5％ | + | ||
| 6.25％ | + | ||
| Propolis (g/100ml) | 1％ | - | |
| 0.5％ | - | ||
| 0.25％ | + | ||
| Ag+(μg/l) | 12.5 | + | |
| 50 | - |
Annotate: the tilt-pour process count plate is cultivated the back: the asepsis growth or 1-5 the bacterium colony of only growing be designated as-and, showing has inhibitory action; Mark+show there is not inhibitory action.
Table 1 result shows: multiple emulsion is 1: 16 to the minimal inhibitory concentration of Escherichia coli 8099, the concentration of propolis is 0.125% (g/100ml) in the multiple emulsion after this dilution, concentration of silver ions is 3.125 μ g/l, and the multiple emulsion under this concentration conditions just has bacteriostasis; And use separately fungistatic effect is arranged just when propolis and silver ion MIC are respectively 0.5% (g/100ml) and 50 μ g/l, separately the component Mlc be multiple emulsion concentration 4-8 doubly.Table 1 result shows Tween-80 contained in the multiple emulsion and the no bacteriostasis of ethanol (1.25%) simultaneously, and what bacteriostasis had been described is multiple emulsion.
2. multiple emulsion is to the shortest antibacterial time experiment of Escherichia coli 8099
With 1: 4,1: 8, dilution in 1: 16 was determined the minimum inhibition time with the bacteriostatic test behind the suspension effect different time of dilution and Escherichia coli 8099 with the multiple emulsion of embodiment 2, and contrast antibacterial time of use propolis, silver ion separately.The results are shown in Table 2:
Table 2 multiple emulsion is to the shortest antibacterial time of Escherichia coli 8099
| Be subjected to reagent | The inhibitory action of effect different time (min) | ||||||
| Multiple emulsion | Dilution factor | 1 | 3 | 5 | 10 | 15 | 30 |
| 1∶4 | - | - | - | ||||
| 1∶8 | - | - | - | ||||
| 1∶16 | - | - | - | ||||
| 2% (g/100ml) propolis | + | + | + | + | + | - | |
| Silver ion 50 μ g/l | + | + | + | + | + | - | |
To annotate: after tilt-pour process words bacterium counting was cultivated, the asepsis growth or 1-5 the bacterium colony of only growing be designated as-,+unrestraint effect.
The visible multiple emulsion of table 2 result is 1min to the minimum inhibition time of Escherichia coli 8099, and uses propolis (2%) separately, and the MIC of silver ion (50 μ g/l) is respectively 30min and 15min.The antibacterial time that shows multiple emulsion is used obviously shortening more separately.
3. detect propolis-silver ion nanoemulsion to Escherichia coli 8099, staphylococcus aureus (ATCC6538), Pseudomonas aeruginosa and Candida albicans (ATCC10231) kill the shortest time, kill least concentration and kill logarithm value.Test method the results are shown in Table 3 referring to the suspension quantitative disinfecting test of " disinfection technology standard ".
Table 3 composite Nano emulsion is killed the result of the test of different microorganisms
| The multiple emulsion dilution factor | Tried bacterial classification | The average kill ratio (%) of effect different time | |||
| 1min | 3min | 5min | 10min | ||
| 1∶4 | Escherichia coli 8099 | 100 | 100 | 100 | |
| 1∶8 | 100 | 100 | 100 | ||
| 1∶16 | 100 | 100 | 100 | ||
| 1∶4 | Staphylococcus aureus ATCC6835 | 100 | 100 | 100 | |
| 1∶8 | 100 | 100 | 100 | ||
| 1∶16 | 100 | 100 | 100 | ||
| 1∶4 | Pseudomonas aeruginosa ATCC15442 | 95 | 100 | 100 | |
| 1∶8 | 90 | 100 | 100 | ||
| 1∶16 | 75 | 100 | 100 | ||
| 1∶4 | Candida albicans ATCC10231 | 60 | 70 | 100 | 100 |
| 1∶8 | 35 | 50 | 100 | 100 | |
Annotate: test temperature 18-22 ℃.The average bacterium number of positive controls, Escherichia coli are 2.78 * 10
8Cfu/mL, staphylococcus aureus is 2.92 * 10
8Cfu/mL, Pseudomonas aeruginosa is 2.53 * 10
8Cfu/mL, Candida albicans are 2.56 * 10
7Cfu/mL; Multiple emulsion uses embodiment 1 preparation.
From table 3 data as seen, the multiple emulsion after the dilution is to Escherichia coli, and staphylococcus aureus acts on 1min, Pseudomonas aeruginosa effect 3min, average kill ratio is 100%, and MCC is dilution in 1: 16 of stoste, and killing logarithm value (KL) is 8.48 and 8.42 and 8.31.To Candida albicans effect 5min, average kill ratio can reach 100%.MCC is dilution in 1: 8 of stoste, and killing logarithm value (KL) is 7.56.And use propolis (2g/100ml) and silver ion (50 μ g/l) only to have bacteriostasis and do not have bactericidal action separately.
More than test explanation, utilize composite Nano emulsion that the present invention prepares effectively wide spectrum kill various bacteria such as Escherichia coli, staphylococcus aureus, Pseudomonas aeruginosa and fungi Candida albicans, virus and bacillus brood cell etc., and use separately propolis and silver ion solution only bacteriostasis to be arranged and do not have bactericidal action, illustrate that activity that propolis silver ion composite nanometer disinfectant of the present invention is killed various pathogenic microorganismss derives from the synergistic function of each component of multiple emulsion of assembly of the present invention with concentration.
The acute toxicity experience of propolis of the present invention and silver ion composite nanometer disinfectant
This experiment is carried out according to " disinfection technology standard " specific requirement.Respectively the multiple emulsion of embodiment 1 and embodiment 3 is tested.
1, acute oral toxicity test: choose 20 of body weight 18～22g kunming mouses, male and female half and half.Be divided into 2 groups at random, 10 every group.Irritate the preceding overnight fasting of stomach, press the disposable filling stomach of maximum dose 5000mg/kg, experimental group is irritated multiple emulsion stoste, and control group is irritated physiological saline.Observe poisoning manifestations, death time and the dead number of elements of mouse in the 14d.The results are shown in Table 4
Table 4 composite Nano emulsion per os toxicity result
| Group | The reaction number of animals | Behavioral activity | Motion | Dead (in the 14d) |
| Composite Nano emulsion stoste group | 0/10 | No abnormal | No abnormal | 0/10 |
| The physiological saline control group | 0/10 | No abnormal | No abnormal | 0/10 |
The result shows, the LD of composite Nano emulsion
50Greater than 10000mg/kg,, belong to actual avirulence.
2. skin irritatin test: choose 3 purebred Japan large ear rabbits.24h before the test, the unhairing left and right sides, backbone both sides Ge Yue 3cm * 3cm, not injured skin.Stoste 0.5ml is dripped in the left side, and the physiological saline that 0.5ml is dripped on the right side in contrast.Behind the 4h, remove the residual thing that tried with warm water.Observe the reaction of 1h, 24h and 48h local skin.Evaluate stimulus intensity by degree score and calculating skin irritation index that erythema and oedema form.The results are shown in Table 5.
Table 5 skin irritatin result of the test
| Group | The skin irritatin reaction: erythema and oedema form | Stimulate average integral | Skin irritation index | Stimulus intensity | ||
| The multiple emulsion group | 1h does not have | 24h does not have | 48h does not have | 0 | 0 | Non-stimulated |
| Control group | Do not have | Do not have | Do not have | 0 | 0 | Non-stimulated |
The result shows that composite Nano emulsion belongs to actual no skin irritation.
3. acute eye irritation test: choose 3 no abnormal purebred Japan large ear rabbits of eyes.Draw the 0.1ml multiple emulsion and splash in the white rabbit conjunctiva of left eye capsule, right eye compares with physiological saline.After splashing into, the passive 4s that closes one's eyes uses normal saline flushing behind the 30s.24h, 48h and 72h judge the stimulation degree of multiple emulsion to eyes with the average score of slit lamp observation white rabbit eye iris, corneal injury and conjunctival congestion, 4 aspects of oedema behind eye drip.The results are shown in Table 6.
Table 6 acute eye irritation test result
| Group | Eye iris, cornea and conjunctiva infringement and congested situation | Stimulate scoring | Stimulate classification | ||
| 24h | 48h | 72h | |||
| Be subjected to examination eye side | Do not have | Do not have | Do not have | 0 | Non-stimulated |
| Contrast eye side | Do not have | Do not have | Do not have | 0 | Non-stimulated |
The result shows that multiple emulsion is to stimulation average integral=0 of rabbit corneal, conjunctiva and iris, no eye irritation.
4. vaginal mucomembranous irritant test: choose 6 purebred female Japan large ear rabbits of health, each 3 of experimental group and control groups.Multiple emulsion or physiological saline 2ml are slowly injected vagina.Perfusion back 24h puts to death animal, takes out complete vagina, and the congestion and edema performance is observed in rip cutting, and fixing 3d in 10% formalin gets the upper and lower end and the central part histologic section of vagina, and after the HE dyeing, pathology detect.According to the situation scoring of epithelial tissue, leukocyte infiltration, the congestion of blood vessel and oedema, calculate the evaluation of average mark and stimulation index to the vaginal mucosa irritation response intensity.The results are shown in Table 7.
Table 7 vaginal mucomembranous irritant test result
| Group | The vagina tissue reaction | The reaction score | Stimulation index | Response intensity | |||
| Epithelial tissue | Leukocyte infiltration | The congestion of blood vessel | Oedema | ||||
| Organized by examination | Intact | Do not have | Do not have | Do not have | 0 | ＜1 | Non-stimulated |
| Control group | Intact | Do not have | Do not have | Do not have | 0 | ＜1 | Non-stimulated |
The result shows, multiple emulsion is to vaginal mucosa irritation index＜1, no mucous membrane irritation.
The acute toxicity experience comprehensive evaluation result: composite Nano emulsion safety of the present invention, avirulence and have no stimulation can and have the characteristics of protective effect in skin, the safe handling of mucous membrane multipath of human body.The former infection of bio-terrorism, morbidity can be effectively prevented and suppress, and the new disinfectant of sterilization can be implemented environment, civilian installation and the precision instrument etc. that pollute.
Claims (9)
1, a kind of propolis and silver ion composite nanometer disinfectant is characterized in that, it is that to contain concentration be that propolis and the concentration of 0.125～2g/100ml average grain diameter between 30～100 nanometers is the oil-in-water emulsion of the silver ion of 3.125～50 μ g/l.
2, propolis according to claim 1 and silver ion composite nanometer disinfectant is characterized in that, also comprise organic solvent, and described organic solvent is ethanol, methyl alcohol, a kind of in n-butanol or the acetone.
3, propolis according to claim 1 and 2 and silver ion composite nanometer disinfectant is characterized in that, used emulsifier is a kind of in Tween-20, Tween-60 or the Tween-80 in the described emulsion.
4, propolis according to claim 1 and 2 and silver ion composite nanometer disinfectant is characterized in that, described silver ion comes from silver nitrate or silver orthophosphate.
5, propolis according to claim 1 and 2 and silver ion composite nanometer disinfectant, it is characterized in that, in per 100 parts by volume emulsions, contain 2 weight portion propolis, 5 parts by volume ethanol, 10～15 parts by volume Tween-80s, 5 * 10-6 weight portion silver nitrate, and when parts by volume was milliliter, weight portion corresponded to gram, and the rest may be inferred.
6, the preparation method of arbitrary described propolis of a kind of claim 1 to 5 and silver ion composite nanometer disinfectant, comprise following operation: propolis is dissolved in the organic solvent, under the room temperature condition, emulsifier is added dropwise in this propolis organic solution, carry out magnetic agitation simultaneously, form oil phase until the mixed liquor clarification, drip silver ionized water solution then while stirring and be described disinfectant until final formation multiple emulsion.
7, the preparation method of propolis according to claim 6 and silver ion composite nanometer disinfectant is characterized in that, emulsifier is added dropwise to rate of addition is 1～2ml/min in the propolis organic solution; Rate of addition is 3～5ml/min when dripping silver ionized water solution.
According to the preparation method of claim 6 or 7 described propolis and silver ion composite nanometer disinfectant, it is characterized in that 8, the volume ratio scope of described emulsifier and propolis organic solution is 1: 1～2: 1.
According to the preparation method of claim 6 or 7 described propolis and silver ion composite nanometer disinfectant, it is characterized in that 9, the volume ratio scope of the oil phase that water that described silver ionized water solution forms and emulsifier and propolis organic solution form is 7: 3～4: 1.
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| CN102579702B (en) * | 2012-03-14 | 2015-07-08 | 山东赛克赛斯药业科技有限公司 | Medicine for treating dental ulcer and preparation method thereof |
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| PL228642B1 (en) | 2014-12-06 | 2018-04-30 | Peszke Jerzy Przemyslaw | Propolis-silver formulation accelerating soft tissue regeneration and method for obtaining it |
| CN106852344A (en) * | 2016-11-22 | 2017-06-16 | 戴琪 | A kind of preparation method of safty of drinking water disinfectant |
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