CN1275515C - Method for refining seeding in cell engineering - Google Patents
Method for refining seeding in cell engineering Download PDFInfo
- Publication number
- CN1275515C CN1275515C CN200410064730.4A CN200410064730A CN1275515C CN 1275515 C CN1275515 C CN 1275515C CN 200410064730 A CN200410064730 A CN 200410064730A CN 1275515 C CN1275515 C CN 1275515C
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- China
- Prior art keywords
- cell engineering
- seedling
- cup
- plant cell
- hardening
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000007670 refining Methods 0.000 title claims description 6
- 238000010899 nucleation Methods 0.000 title claims description 5
- 229910052902 vermiculite Inorganic materials 0.000 claims description 12
- 235000019354 vermiculite Nutrition 0.000 claims description 12
- 239000010455 vermiculite Substances 0.000 claims description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 239000007921 spray Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000011159 matrix material Substances 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 4
- 235000008935 nutritious Nutrition 0.000 claims description 4
- 244000037666 field crops Species 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 2
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 2
- 239000006013 carbendazim Substances 0.000 claims description 2
- 230000000249 desinfective effect Effects 0.000 claims description 2
- 239000008187 granular material Substances 0.000 claims description 2
- 239000012286 potassium permanganate Substances 0.000 claims description 2
- 239000008223 sterile water Substances 0.000 claims description 2
- 238000013019 agitation Methods 0.000 claims 2
- 239000000645 desinfectant Substances 0.000 claims 1
- 230000008569 process Effects 0.000 abstract description 7
- 230000004083 survival effect Effects 0.000 abstract description 6
- 239000000463 material Substances 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 230000006978 adaptation Effects 0.000 abstract 3
- 230000007774 longterm Effects 0.000 abstract 1
- 230000001932 seasonal effect Effects 0.000 abstract 1
- 238000000889 atomisation Methods 0.000 description 5
- 239000010410 layer Substances 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000010923 batch production Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 231100000225 lethality Toxicity 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000003716 rejuvenation Effects 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 229920001875 Ebonite Polymers 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 235000011089 carbon dioxide Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000012407 engineering method Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000011229 interlayer Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The present invention belongs to the technical field of plant cell engineering. The present invention solves the problem of low survival rate for plant cell engineering seedlings during the process of seedling adaptation, which has puzzled scientific and technological workers for a long term. The present invention ensures that the plant cell engineering seedlings live through the seedling adaptation stage easily so as to improve the survival rate greatly. The present invention relates to a technique for indoor three-dimensional industrial seedling adaptation. The present invention can save area, avoid seasonal interference, prolong production time, and exert the advantages of the plant cell engineering seedlings fully. Materials used in the present invention can be repeatedly utilized, so the materials are sustainable.
Description
One, technical field
This invention belongs to field of plant cell engineering technology.
Two, background technology
The plant cell engineering seedling must pass through this significant process of hardening from the laboratory before big Tanaka's cultivation.And general hardening off method usually makes the lethality of the plant cell engineering seedling that has just shifted out from this fat city of medium very high, this problem fails to be well solved for a long time, the advantage that is difficult to real plant cell engineering seedling has hindered the promotion and application of this clone's raising technology of plant cell engineering seedling.
The plant cell engineering seedling was very delicate when existing hardening off method failed to solve just from media transfer to the field, the problem of bad adaptability.Therefore, existing hardening off method very easily makes the plant cell engineering seedling occur withering or rots, and lethality is very high.The present invention can increase substantially the survival rate in the plant cell engineering seedling refining process, and the large tracts of land of this no sex clone raising technology of plant cell engineering seedling is promoted provides reliable guarantee.The real plant cell engineering seedling of realizing can expand numerous advantage fast, has also reduced the cost of producing the plant cell engineering seedling simultaneously, for the practical application of this quick multiplication technique provides wide prospect.
Still there are not this plant cell engineering method for refining seeding research and relevant report at present both at home and abroad.
Three, summary of the invention
(1) the present invention's problem that need solve is:
1. in the hardening process, the plant cell engineering seedling is more delicate, and very easily dehydration withers
2. the process from media transfer to hardening alms bowl body, cause that easily seedling hinders root.
3. avoid the harmful effect of rhythm and pace of moving things pair cell engineering seedling hardening in season.
4. solve general hardening and take up an area of many problems, realize indoor, three-dimensional intensification, batch production hardening.
(2) concrete technical scheme of the present invention is:
We are at these characteristics of plant cell engineering seedling, having invented in plant cell engineering seedling hardening process can be for it provides good environmental condition such as suitable temperature, humidity, illumination, carbonic acid gas, oxygen and root, foliar nutrition, has also designed to be the diseases prevention of plant cell engineering seedling, the scheme of curing the disease.For the plant cell engineering seedling provides good animation, impel growing and rejuvenation of plant cell engineering shoot root, stem, leaf, improve its adaptability and resistance, improve its survival rate greatly, lay a solid foundation for the plant cell engineering seedling can large batch ofly move towards the land for growing field crops from the laboratory.Concrete technical scheme is as follows:
1. the preparation of hardening device and debugging
2. cultivate alms bowl body and matrix treatments
3. the processing of plant cell engineering seedling and transplanting
4. hardening.
(2) the present invention compared with the prior art, its significance progress is:
1. the present invention utilizes gas permeability, good water absorption and material that can autoclave sterilization to make matrix,, makes seedling be in the wetting comfortable air and carries out photosynthesis for the plant cell engineering seedling provides superfine little vaporific liquid with the self-timing atomization system.And in conjunction with root, blade face for fertilizer and sterilization measure, guarantee that the plant cell engineering seedling tides over the hardening phase smoothly.The survival rate of plant cell engineering seedling can be reached more than 90%, improve the survival rate of its hardening greatly.
2. the present invention is few to plant cell engineering seedling injury, and can survive for the plant cell engineering seedling provides suitable in the hardening process, growth, rejuvenation condition.
3. the present invention is not subjected to the restriction in season, can carry out the hardening of plant cell engineering seedling throughout the year, has prolonged the production time greatly.
4. this method adopts indoor stereo multilayer posture hardening, can be in enormous quantities, batch production, intensification hardening, solved the problem more than hardening is taken up an area of.
5. the used material economy of this method is easy to get and can repeatedly recycles, and has sustainability etc.
Four, embodiment
(1) preparation of hardening device and debugging: buy the shelf angle steel,, be processed into the multiple field cabinet-type framework of certain size size according to the place size; About the frame body, about and rear side with the sealing of the high plastic film of light transmittance; Each interlayer is made collet with hard rubber sheet, and makes a call to a certain size a circular hole in the central, slightly is longer than support body and circular hole to one and passes circular hole with the plastic tube in footpath, makes pipeline be positioned at the central authorities of support body.And then the pipeline of each layer upper end makes a call to all apertures in the place slightly down, and the nozzle of atomizer is connected with support body lower end of duct mouth; On every layer one side top a miniature air perturbator is installed; Mobilizable door curtain is made with plastic foil in the front that framework is every layer.Be placed on and see that sunlight place or every layer of installation fluorescent tube replenish illumination.Debug suitable atomization quantity and time set, the alcohol with 70% sprays each layer sterilization and ultraviolet lamp disinfection processing back is standby.
(2) cultivation alms bowl body and matrix treatments: buy the disposal plastic cup of the excellent 250ml of quality, in its bottom with beat some small garden holes on every side; With 2mm sieve vermiculite being sieved is divided into thick, thin two parts, standby behind the autoclave sterilization.Earlier the coarse granule vermiculite is put into cup to 2/3 height, the thin vermiculite of one deck is spread on the surface, and the sterilization ling that sprays 500ppm is stand-by.
(3) processing of plant cell engineering seedling and transplanting: cultured plant cell engineering seedling of taking root good taken out from medium put into clear water, the careful medium of removing root, use disinfecting solution of potassium permanganate, with after the rinsed with sterile water it being put on the thin vermiculite of above-mentioned cup gently, around seedling, spread thin vermiculite lightly with populated around its root, under honest several gently on the table, it is high by about 4/5 to cup to spread thick vermiculite again above it again, then cup is emitted in the pallet of nutritious liquid (seeing Table 1) and soaks into, also can drip nutrient solution to running through at the cup face simultaneously.To the carbendazim solution of seedling and rim of a cup face sprinkling 500ppm, it is emitted in the hardening device at last.
Table 1 is supported the liquid composition proportion
| Composition | Dosage (g/L) | Composition | Dosage (g/L) | ||
| 1 | KH2PO 4 | 0.14 | 7 | Ironic citrate | 0.02 |
| 2 | KNO 3 | 0.51 | 8 | ZnSO 4·7H 2O | 0.22 |
| 3 | Ca(NO 3) 2 | 0.82 | 9 | CuSO 4·5H 2O | 0.08 |
| 4 | MgSO 4 | 0.49 | 10 | H 2MO 4·H 2O | 0.02 |
| 5 | H 3BO 3 | 2.86 | |||
| 6 | MnCl 2·4H 2O | 1.81 |
(4) hardening: atomizer and miniature air perturbator are set at regularly alternation switch of circulation in 24 hours, and promptly atomisation, miniature air perturbator stop, and---atomizer stops spraying, the miniature air perturbator starts, and---atomisation, miniature air perturbator stop---atomizer stops spraying, the miniature air perturbator starts---...Switch time, length can be decided as the case may be.Daytime, spray time was short at interval, and evening, spray time was long at interval; Behind the water of atomizing regular period, insert the primary atomization nutrient solution.
(5) change basin: hardening carefully was transplanted to seedling in the cup of nutritious matrix after 10 to 15 days, continued hardening after 5 to 10 days, seedling is taken out in the lump together with medium move into the land for growing field crops.
Claims (1)
1, a kind of hardening off method of cell engineering Seedling, it is characterized in that utilizing the refining seeding in cell engineering device of being made up of atomizer, nozzle, air agitation device, timing control switch, cabinet-type framework to provide exercise from blake bottle to big Tanaka's existence for cell engineering Seedling, concrete grammar is as follows:
(1) bottom and the sidewall that will be used for cultivating the alms bowl body of cell engineering Seedling stamped aperture;
(2) the refining seeding in cell engineering device is with 70% alcohol spray disinfectant, ultraviolet disinfection;
(3) with 2mm sieve vermiculite is sieved and be divided into thick, thin two parts, after the sterilization, the coarse granule vermiculite is put into cup to 2/3 height, the thin vermiculite of one deck is spread on the surface, and the sterilization ling that sprays 500ppm is stand-by;
(4) the plant cell engineering seedling that will take root good takes out from medium and puts into clear water, remove the medium of root, use disinfecting solution of potassium permanganate, with putting it into after the rinsed with sterile water in the above-mentioned cup on the thin vermiculite, around seedling, spread thin vermiculite with populated around its root, it is high by 4/5 to cup to spread thick vermiculite in the above, then cup is emitted in the pallet of nutritious liquid to soak into, and drips nutrient solution to running through at the cup face simultaneously;
(5) to the carbendazim solution of seedling and rim of a cup face sprinkling 500ppm, be emitted in the hardening device;
(6) air agitation device, atomizer circulation timing in 24 hours alternation switch;
(7) hardening is 10～15 days, is transplanted in the cup of nutritious matrix, continues hardening after 5-10 days, and seedling and medium are taken out the immigration land for growing field crops in the lump.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200410064730.4A CN1275515C (en) | 2004-09-24 | 2004-09-24 | Method for refining seeding in cell engineering |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200410064730.4A CN1275515C (en) | 2004-09-24 | 2004-09-24 | Method for refining seeding in cell engineering |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1600081A CN1600081A (en) | 2005-03-30 |
| CN1275515C true CN1275515C (en) | 2006-09-20 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200410064730.4A Expired - Fee Related CN1275515C (en) | 2004-09-24 | 2004-09-24 | Method for refining seeding in cell engineering |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1275515C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11945186B2 (en) | 2019-08-08 | 2024-04-02 | Azionaria Costruzioni Macchine Automatiche A.C.M.A. S.P.A. | Machine and method for forming containers from blanks |
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2004
- 2004-09-24 CN CN200410064730.4A patent/CN1275515C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11945186B2 (en) | 2019-08-08 | 2024-04-02 | Azionaria Costruzioni Macchine Automatiche A.C.M.A. S.P.A. | Machine and method for forming containers from blanks |
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| Publication number | Publication date |
|---|---|
| CN1600081A (en) | 2005-03-30 |
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Granted publication date: 20060920 |