CN1273604A - 抗冻蛋白、dna及其表达体系 - Google Patents

抗冻蛋白、dna及其表达体系 Download PDF

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Publication number
CN1273604A
CN1273604A CN98809742A CN98809742A CN1273604A CN 1273604 A CN1273604 A CN 1273604A CN 98809742 A CN98809742 A CN 98809742A CN 98809742 A CN98809742 A CN 98809742A CN 1273604 A CN1273604 A CN 1273604A
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CN
China
Prior art keywords
polypeptide
plant
protein
dna
chitinase
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Pending
Application number
CN98809742A
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English (en)
Chinese (zh)
Inventor
乔伊·休
熊非
芭芭拉·莫法特
玛丽莲·格里菲思
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ICE BIOTECH Inc
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ICE BIOTECH Inc
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Publication date
Application filed by ICE BIOTECH Inc filed Critical ICE BIOTECH Inc
Publication of CN1273604A publication Critical patent/CN1273604A/zh
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2442Chitinase (3.2.1.14)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01014Chitinase (3.2.1.14)
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • A01K2217/05Animals comprising random inserted nucleic acids (transgenic)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
CN98809742A 1997-07-31 1998-07-31 抗冻蛋白、dna及其表达体系 Pending CN1273604A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US90387297A 1997-07-31 1997-07-31
US08/903,872 1997-07-31

Publications (1)

Publication Number Publication Date
CN1273604A true CN1273604A (zh) 2000-11-15

Family

ID=25418191

Family Applications (1)

Application Number Title Priority Date Filing Date
CN98809742A Pending CN1273604A (zh) 1997-07-31 1998-07-31 抗冻蛋白、dna及其表达体系

Country Status (5)

Country Link
EP (1) EP1002101A2 (fr)
CN (1) CN1273604A (fr)
AU (1) AU8620698A (fr)
CA (1) CA2299620A1 (fr)
WO (1) WO1999006565A2 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104975034A (zh) * 2015-06-25 2015-10-14 吉林省农业科学院 羊草几丁质酶LcChi2基因在提高植物耐冷性方面的应用
CN111471082A (zh) * 2019-01-24 2020-07-31 华东师范大学 一种萝卜苗质外体抗冻蛋白的提取方法
CN111896742A (zh) * 2015-07-07 2020-11-06 思齐乐 流感效力试验
CN117164674A (zh) * 2023-10-24 2023-12-05 上海水大技术转移有限公司 一种抗冻蛋白及其基因、酵母工程菌和应用

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000049044A1 (fr) * 1999-02-19 2000-08-24 Astrazeneca Ab Expression de polypeptides helicobacter dans pichia pastoris
JP3234897B2 (ja) * 1999-03-25 2001-12-04 農林水産省北海道農業試験場長 低温発現型キチナーゼ遺伝子およびその単離方法
EP1181865A1 (fr) * 2000-08-23 2002-02-27 Universite Catholique De Louvain Solutions cryoprotectrices
WO2003055320A1 (fr) * 2002-01-04 2003-07-10 Unilever Nv Proteines antigel dans des legumes
US7132263B2 (en) 2002-09-09 2006-11-07 Agrigenesis Biosciences Limited Antifreeze proteins isolated from forage grasses and methods for their use
US7968767B2 (en) 2002-09-09 2011-06-28 Jeroen Demmer Antifreeze proteins isolated from forage grasses and methods for their use
KR100543063B1 (ko) * 2004-03-16 2006-01-20 (주)넥스젠 재조합 단백질의 제조 및 분리 방법
ES2334741B1 (es) * 2008-06-26 2011-02-17 Consejo Superior De Investigaciones Cientificas (Csic) Proteina crioprotectora con actividad quitinasa a bajas temperaturas,procedimiento para su obtencion y sus aplicaciones.
ES2334743B1 (es) * 2008-06-27 2011-02-17 Consejo Superior De Investigaciones Cientificas (Csic) Proteina crioprotectora con actividad 1-3 b - glucanasa a bajas temperaturas, procedimiento para su obtencion y sus aplicaciones.
KR20240046159A (ko) * 2021-06-15 2024-04-08 글로바켐 엔브이 키틴분해 효소-기반 식물 보호제

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5554521A (en) * 1984-03-26 1996-09-10 Dna Plant Technology Corporation Chitinase-producing plants
ES2223041T3 (es) * 1991-06-13 2005-02-16 Microstar Biotech Inc. Tolerancias al frio en plantas.
US5627051A (en) * 1995-06-07 1997-05-06 University Of Notre Dame Du Lac Nucleic acid sequences encoding dendroides antifreeze proteins
SK9099A3 (en) * 1996-07-26 1999-06-11 Unilever Nv Frozen confectionery products

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104975034A (zh) * 2015-06-25 2015-10-14 吉林省农业科学院 羊草几丁质酶LcChi2基因在提高植物耐冷性方面的应用
CN104975034B (zh) * 2015-06-25 2018-11-27 吉林省农业科学院 羊草几丁质酶LcChi2基因在提高植物耐冷性方面的应用
CN111896742A (zh) * 2015-07-07 2020-11-06 思齐乐 流感效力试验
CN111471082A (zh) * 2019-01-24 2020-07-31 华东师范大学 一种萝卜苗质外体抗冻蛋白的提取方法
CN117164674A (zh) * 2023-10-24 2023-12-05 上海水大技术转移有限公司 一种抗冻蛋白及其基因、酵母工程菌和应用
CN117164674B (zh) * 2023-10-24 2024-02-02 上海水大技术转移有限公司 一种抗冻蛋白及其基因、酵母工程菌和应用

Also Published As

Publication number Publication date
WO1999006565A3 (fr) 1999-06-03
CA2299620A1 (fr) 1999-02-11
AU8620698A (en) 1999-02-22
EP1002101A2 (fr) 2000-05-24
WO1999006565A2 (fr) 1999-02-11

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