CN1255528C - Method for producing bush mycorrhizal fungi preparation - Google Patents
Method for producing bush mycorrhizal fungi preparation Download PDFInfo
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- CN1255528C CN1255528C CN 02159722 CN02159722A CN1255528C CN 1255528 C CN1255528 C CN 1255528C CN 02159722 CN02159722 CN 02159722 CN 02159722 A CN02159722 A CN 02159722A CN 1255528 C CN1255528 C CN 1255528C
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- mycorrhizal fungi
- bush mycorrhizal
- culture medium
- microbial inoculum
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Abstract
The present invention relates to a method for producing bush mycorrhizal fungus preparations. A propagule fungus preparation with bush mycorrhizal fungi, which is equivalent to 5% to 10% of the volume of base material, is inoculated on a culture medium composed of 15 to 25 portions of grass carbon, 15 to 20 portions of vermiculite, 30 to 45 portions of perlite, and 15 to 25 (weight) portions of zeolite; the mode of the mix inoculation of various host plants is adopted for cultivation; nutrient solution is watered once each 2 to 3 days; after the host plants are cultured for 17 to 18 weeks, the culture medium and plant roots are collected. The culture medium provided by the present invention has the advantages of easy acquisition, simple device, low cost, advanced production technology and environmental friendliness.
Description
Technical field:
The present invention relates to a kind of method of producing the bush mycorrhizal fungi microbial inoculum.
Background technology:
Bush mycorrhizal fungi (Arbuscular mycorrhizal fungus) is the fungi that a class can form symbiotic relationship with the land plant more than 80%, it can help and promote nutritive elements such as plant absorbing phosphorus, zinc, copper, potassium and boron, significantly improve the nutritional status of plant, and can improve plant drought-resistant, saline and alkaline, resistance against diseases, improve Soil structure.
The conventional cultural method of bush mycorrhizal fungi mainly is that employing soil or river sand are matrix, and monocrop (as corn, trifolium) is a host plant.The culture efficiency of mycorrhizal fungi can be improved by composition and the culture condition of regulating culture medium.Atimanav etc. have compared influence (the Atimanav Gaur of the expansion mineral material of different-grain diameter to bush mycorrhizal fungi production, A.dholeya Effects of theparticle size of soil-less substrates upon AM fungus inoculumproduction 2000,10:43-48); Britain PLANT WORK company (www.plantworksuk.co.uk) adopts a kind of expansion mineral to cultivate bush mycorrhizal fungi as matrix, has used the growth of the control of slow-releasing phosphate fertilizer and adjusting plant and mycorrhizal fungi simultaneously in culturing process.These methods adopt single expansion mineral or expansion mineral to add the mode of careless carbon mostly, and culture efficiency is not high enough.Document (Sylvia, D.M.and D.H.Hubbell.1986.Growth and sporulationof vesicular-arbuscular mycorrhizal fungi in aeroponic and membranesystems.Symbiosis 1:259-267) adopt aeroponics to cultivate microbial inoculum, in airtight container, nutritive medium is become aerosol and be sprayed on and infect on the root system of plant that mycorrhizal fungi is arranged, obtain purified microbial inoculum with atomisation unit.The advantage of this method is to obtain very purified microbial inoculum, but the cost height, yields poorly.
It all is to adopt under greenhouse experiment host plant and 4～5 months mode of bush mycorrhizal fungi co-cultivation to be carried out that the bush mycorrhizal fungi microbial inoculum is commercially produced.Because bush mycorrhizal fungi can not leave the host plant single culture, so this microbial inoculum scale operation is very difficult; And the type of host plant, the prescription of culture medium, culture condition are the key of production technique.
Summary of the invention:
The technical problem to be solved in the present invention provides the method for commercially producing the bush mycorrhizal fungi microbial inoculum.
The technical solution used in the present invention is:
By 15～25 parts in careless carbon, vermiculite 15-20 part, 30～45 parts of perlites, in the culture medium that zeolite 15-25 part weight part is formed, add the sodium phytate that is equivalent to the total umber 0.01-0.1 of matrix, what inoculation was equivalent to matrix volume 5～10% contains Glomus mosseae, Glomus versiforme, three kinds of microbial inoculums that mix the propagulum of bush mycorrhizal fungi of Glomus intraradices, select jowar, corn, trifolium, 2～4 kinds in the onion as host plant, in temperature is 25-35 ℃, and relative humidity 50%-80% cultivates host plant and mycorrhizal fungi symbiote under the condition of intensity of illumination 10000-20000 lux; Watered one time of nutrition liquid in per 2～3 days between incubation period, the concentration of various compositions is in the nutritive medium: KH
2PO
43.0 * 10
-2Mmol/L, NH
4NO
34mmol/L, MgSO
42.6mmol/L, Ca (NO
3)
28.0mmol/L, sylvite 8-11mmol/L, boric acid or salt 1.0 * 10
-3Mmol/L, MnSO
41.6 * 10
-3Mmol/L, ZnSO
41.0 * 10
-3Mmol/L, CuSO
45.0 * 10
-4Mmol/L, (NH
4)
6MO
2O
45.0 * 10
-2Mmol/L, iron-retention agent 2.0 * 10
-2Mmol/L; , the pH value of nutritive medium is 6.2-6.5; Select multiple host plant mixed seeding to mix up mode; After cultivating for 17～18 weeks, collection culture medium and root system of plant.
Sylvite described in the nutritive medium is K
2SO
4, KCl or KNO
3In one or more.
Iron-retention agent described in the nutritive medium is Fe-EDTA or Fe-DTPA.
Borate described in the nutritive medium is Na
3BO
3
The present invention's beneficial effect against existing technologies is:
1, culture medium obtains easily, and equipment is simple, and is with low cost;
2, production technique advanced person;
3, environmental friendliness.
The mycorhiza biotechnological formulation that present technique is produced can replace phosphate fertilizer and micro-chemical fertilizer to a certain extent, improve plant is infected disease and arid to soil resistivity, thereby reduce the pollution of chemical fertilizer and agricultural chemicals, be applicable to and transplant class vegetables, medicinal material and other farm crop environment.
Embodiment:
The production of embodiment 1 bush mycorrhizal fungi microbial inoculum
(1) matrix preparation
Careless carbon was carried out sterilising treatment 1～2 hour in 121 ℃, the high-pressure sterilizing pot of 0.12MPa, dry; Vermiculite, perlite and zeolite be with 3% salt acid soak 0.5～1 hour, eluriates totally with clear water, dries then.
With careless carbon: vermiculite: perlite: zeolite 1: 1: 2: 1 volume ratio mixes, and adds the sodium phytate that is equivalent to the total umber 0.01-0.1 of matrix part, once more mixing.
(2) inoculation, plantation phytoparasite
In the greenhouse, build the high cement pit of 50cm or with polyethylene plastic film and brick build up can water leakage the pond.Above-mentioned mixed-matrix is packed in the pond, and the degree of depth is 30-40cm.
Glomus mosseae will be contained respectively, Glomus versiform, three kinds of Glomus intraradices mix mycorrhizal fungal spore, mycelial Inoculant (spore density of every bacterial classification all 〉=25/gram) and mix earlier, and then the Inoculant that will mix is layered on the matrix, total Inoculant consumption is equivalent to 7% of the total umber of matrix, is covered with the matrix of one deck 2cm again.Broadcast sorghum seeds by 20 * 20 spacing punching then, around it, broadcast trifolium, onion.1 young plant is stayed in the every cave of jowar after waiting to emerge, trifolium and not thinning of onion.
(3) cultivate
Temperature between incubation period is 25-35 ℃, relative humidity 50%-80%, intensity of illumination 10000-20000 lux.Watered one time of nutrition liquid in every 2-3 days in the plant back of emerging.
The concentration of the various compositions in the nutritive medium is: KH
2PO
43.0 * 10
-2Mmol/L, NH
4NO
34mmol/L, MgSO
42.6mmol/L, Ca (NO
3)
28.0mmol/L, K
2SO
43mmol/L, KCl 8mmol/L, H
3BO
31.0 * 10
-3Mmol/L, MnSO
41.6 * 10
-3Mmol/L, ZnSO
41.0 * 10
-3Mmol/L, CuSO
45.0 * 10
-4Mmol/L, (NH
4)
6MO
2O
45.0 * 10
-2Mmol/L, Fe-EDTA 2.0 * 10
-2Mmol/L.Nutritive medium pH is 6.2-6.5.
(4) results
Gather in the crops after plant strain growth 17-18 week.During results, cut off overground part earlier, scrape off matrix top layer 2cm, the matrix of lower floor is dried together with root system of plant, again root system is ground into broken section less than 0.5cm, with the abundant mixing of matrix, pack.
Detect propagulum quantity with the MPN method, reach more than 10/ gram; Detect the propagulum activity with psyllium (Plantagolanceolata) for host plant, the infection rate of inoculating 21 days reaches more than 30%.Reach these standards and be qualified product.
It should be noted last that: above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although the present invention is had been described in detail with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can make amendment or be equal to replacement technical scheme of the present invention, and not breaking away from the spirit and scope of technical solution of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.
Claims (4)
1, a kind of method of producing the bush mycorrhizal fungi microbial inoculum, it is characterized in that: by 15～25 parts in careless carbon, 15～20 parts of vermiculites, 30～45 parts of perlites, in the culture medium that 15～25 parts of weight parts of zeolite are formed, add the sodium phytate that is equivalent to the total umber 0.01-0.1 of matrix part, what inoculation was equivalent to matrix volume 5～10% contains Glomus mosseae, Glomus versiforme, three kinds of microbial inoculums that mix the propagulum of bush mycorrhizal fungi of Glomus intraradices, select jowar, corn, trifolium, 2～4 kinds in the onion as host plant, in temperature is 25-35 ℃, relative humidity 50%-80% cultivates host plant and mycorrhizal fungi symbiote under the condition of intensity of illumination 10000-20000 lux; Watered one time of nutrition liquid in per 2～3 days between incubation period, the concentration of various compositions is in the nutritive medium: KH
2PO
43.0 * 10
-2Mmol/L, NH
4NO
34mmol/L, MgSO
42.6mmol/L, Ca (NO
3)
28.0mmol/L, sylvite 8-11mmol/L, boric acid or salt 1.0 * 10
-3Mmol/L, MnSO
41.6 * 10
-3Mmol/L, ZnSO
41.0 * 10
-3Mmol/L, CuSO
45.0 * 10
-2Mmol/L, (NH
4)
6MO
2O
45.0 * 10
-2Mmol/L, iron-retention agent 2.0 * 10
-2Mmol/L, the pH value of nutritive medium is 6.2-6.5; Select multiple host plant mixed seeding to mix up mode; After cultivating for 17～18 weeks, collection culture medium and root system of plant.
2, the method for production bush mycorrhizal fungi microbial inoculum according to claim 1, described sylvite is K
2SO
4, KCl or KNO
3In one or more.
3, the method for production bush mycorrhizal fungi microbial inoculum according to claim 1 and 2, described iron-retention agent is Fe-EDTA or Fe-DTPA.
4, the method for production bush mycorrhizal fungi microbial inoculum according to claim 1 and 2, described borate is Na
3BO
3
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02159722 CN1255528C (en) | 2002-12-30 | 2002-12-30 | Method for producing bush mycorrhizal fungi preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02159722 CN1255528C (en) | 2002-12-30 | 2002-12-30 | Method for producing bush mycorrhizal fungi preparation |
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|---|---|
| CN1511944A CN1511944A (en) | 2004-07-14 |
| CN1255528C true CN1255528C (en) | 2006-05-10 |
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| CN 02159722 Expired - Fee Related CN1255528C (en) | 2002-12-30 | 2002-12-30 | Method for producing bush mycorrhizal fungi preparation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9169439B2 (en) | 2012-08-29 | 2015-10-27 | Suncoke Technology And Development Llc | Method and apparatus for testing coal coking properties |
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| CN101990825B (en) * | 2010-08-24 | 2012-04-18 | 上海弘升科技发展有限公司 | Method for preparing mycorrhiza industrial products |
| CN102224787B (en) * | 2011-04-29 | 2013-01-09 | 云南省烟草农业科学研究院 | Method for producing mycorrhizal tobacco seedings by utilizing moist and float seedling culturing manner |
| CN102229893B (en) * | 2011-06-02 | 2012-09-05 | 刘方一 | High-efficiency high-yield production method of mycorrhizal fungi |
| CN102523863A (en) * | 2011-11-18 | 2012-07-04 | 中国中医科学院中药研究所 | Method for cultivating Panax notoginseng by arbuscular mycorrhizal fungi technique |
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| CN103229667B (en) * | 2013-04-27 | 2015-06-10 | 北京林业大学 | Mycorrhizal inoculum and preparing method and use thereof |
| LU92274B1 (en) * | 2013-08-30 | 2015-03-02 | Symplanta Gmbh & Co Kg | System and methods for continuous propagation and mass production of arbuscular mycorrhizal fungi |
| CN104041393A (en) * | 2014-06-13 | 2014-09-17 | 兰州大学 | Fast expanding propagation method for mycorrhiza fungus strain |
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| CN108419605B (en) * | 2018-02-13 | 2019-08-20 | 河南科技大学 | A kind of bush mycorrhizal fungi preparation and the preparation method and application thereof |
| CN109197385B (en) * | 2018-09-20 | 2021-07-02 | 浙江世佳科技股份有限公司 | Arbuscular mycorrhizal fungi culture medium |
| CN112385507B (en) * | 2020-11-16 | 2021-07-23 | 南京农业大学 | Seedling culture medium containing arbuscular mycorrhiza and preparation method and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9169439B2 (en) | 2012-08-29 | 2015-10-27 | Suncoke Technology And Development Llc | Method and apparatus for testing coal coking properties |
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| CN1511944A (en) | 2004-07-14 |
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