CN1255142C - Chinese traditional medicine mixer for preventing and treating infectious chicken bursa of Fabricius disease - Google Patents
Chinese traditional medicine mixer for preventing and treating infectious chicken bursa of Fabricius disease Download PDFInfo
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Abstract
The present invention relates to a traditional Chinese medicine mixture for preventing and treating chicken infectious Fabricius bursal diseases, which is named Qilankangduyin. The traditional Chinese medicine mixture mainly comprises the following components: astragalus, isatis root, indigowood leaf, dried rehmannia root, red peony root, etc. Comparison tests on the treatment of counteracting toxic substances prove that in six different processing groups, the mixture processing group has the best curative effect, the least pathological bleeding and the most obvious weight increment effect. Comparison tests on the prevention of counteracting toxic substances prove that the mixture can reduce mortality and can improve specific antibody levels whether the mixture is used alone or used together with vaccine. The treatment of groups of clinic sick chickens verifies that the protective rates all reach 90% or so. Moreover, the mixture overcomes many defects of other therapeutic medicines, has no toxic side effect or medicine residues, conforms to green medicines and guarantees the requirements of animal source food safety. The medicine passes through the evaluation of new veterinary medicines in Jiangsu province.
Description
One, technical field
A kind of herb mixture of preventing and treating infectious bursal disease of the present invention is named to the blue antitoxin drink of stilbene, belongs to the novel herbal medicine of preventing and treating infectious bursal disease.
Two, technical background
Infectious bursal disease (Infectious bursal disease, IBD), from nineteen fifty-seven since the Gan Buluo town of the Delaware State, east coast of the united states finds first, be worldwide popular.According to investigations, U.S. chicken group's seroprevalence is almost nearly 100%, and South America is about 90%, Holland 88%, and Italy 76%, Japan is about 75%, Germany 61.5%.Zhou Jiao in 1980 etc. were separated to the IBD-CJ801 strain from the chicken group of import in Beijing, thereby confirmed the existence of primary disease in China at Guangzhou discovery primary disease in 1979 in China.
IBD is acute, the height contagious disease of the young chicken of harm.The harm of its maximum is immune maincenter organ---the fabricius bursa that destroys chicken, make the bone-marrow-derived lymphocyte degeneration and the necrosis of carrying the SIgM sign, cause serious, secular immunosuppressant, make body reduce, the susceptibility of pathogen such as other antibacterial and virus is increased the immunoreation of other vaccine.Its morbidity is propagated rapidly suddenly, and is dead in infection beginning in the 3rd day usually, peaks, and stops very soon later in 5～7 days.Sick mortality rate is generally 15%～50%, and virulent infects mortality rate and can reach more than 70%.Popular some new features that occurred of IBD in recent years show as morbidity age in days expanded range, and the chicken that grows up from 3 Japanese instar chicklings to 180 ages in days all can be taken place, and atypical case, mixed infection or syndrome increase, and mortality rate Gao Zheke reaches 90%.
Because IBDV is comparatively stable in external environment, conventional sterilization and quarantine measures can not be controlled primary disease.Main preventive measure is vaccination.Because antigenic diversity between the appearance of superpower IBDV and various serotype and the variant makes vaccine immunity be difficult to obtain ideal effect, can cause other disease on the contrary sometimes in recent years, cause enormous economic loss to poultry husbandry.
IBD there is not specific western medicine so far.Someone uses the treatment of dexamethasone and Norethisterone Compound, but can only mitigation symptoms or mitigate the disease, can not reduce mortality rate.To early stage sick chicken, with high immunity yolk antibody or hyper-immune serum certain curative effect is arranged, but both all are vulnerable to condition effect such as preparation, storage and transport, the danger that effect is unstable or other disease is propagated in existence.
The preparation of high-immunity yolk and serum derives from commercial chicken and egg since immune chicken individuals difference or batch between antibody horizontal differ, make product antibody titer instability.Both are the high protein material, and are nutritious, in process of production polluted bacteria very easily; And may have other cause of disease, as egg drop syndrome, avian leukosis, mycoplasma etc., there is potential threat in aviculture.Yolk antibody contains antigenicity substances such as a large amount of vitellins, fat, and easily the immunity to sick chicken produces interference; The molecular weight of its protein and fat is all very big, and intramuscular injection not only is difficult to absorb, and also can cause local swelling, hemorrhage, downright bad, even cause physical stress and anaphylaxis.To Be Protected from Heat for antibody, must cryopreservation and transportation, and especially very easily putrid and deteriorated and can not use in summer.Therefore, a kind of new more efficiently medicine of searching is imperative from Chinese herbal medicine.
Three, summary of the invention
Technical problem the present invention is directed to the problem of being badly in need of green medicine in the prior art, and a kind of pure Chinese medicinal preparation of preventing and treating infectious bursal disease is provided.It is reliable to reach drug effect, has no side effect no drug residue, the purpose that ensures food safety.
A kind of herb mixture of preventing and treating infectious bursal disease of technical scheme is characterized in that,
The recipe quantity medical material of every 1000ml medicinal liquid is: Radix Astragali 300g, Radix Isatidis 200g, Folium Isatidis 200g, Radix Rehmanniae 200g, Radix Paeoniae Rubra 100g.
The technological process of producing every 1000ml medicinal liquid is: use water extraction.With above five kinds of Chinese medicine, decoct with water twice, each 1 hour, filtration, merging filtrate is concentrated into 1000ml, adds the 4g sodium benzoate, leaves standstill after stirring evenly, and precipitation is removed in filtration or centrifugal, the aseptic subpackaged bottle of going into to sterilize.
Beneficial effect
2000～calendar year 2001, this research department under the subsidy of Jiangsu Province's " three pharmaceutical worker's journeys " project, the research of abduction treatment by Chinese herbs infectious bursal disease novel formulation.At first use the method for cell culture to filter out the stronger Radix Astragali of infections chicken cloacal bursa virus resisting effect as principal agent, the pathology of theoretical according to traditional Chinese veterinary medicine then and infectious bursal disease, take distinct principle to work out three prescriptions, by repetition test relatively, filter out a best prescription of effect at last, naming is the antitoxin drink of stilbene indigo plant.
Counteracting toxic substances treatment comparative test proves (table 1～table 3), the curative effect of the blue antitoxin drink of stilbene is better than other two prescriptions, is better than yolk antibody slightly, the mortality rate of this medicine processed group minimum (18.97%) is lower than yolk antibody matched group (21.03%), extremely significantly is lower than not treat matched group (46.7%); Sick chicken organ hemorrhage positive rate is the lightest, is lighter than the yolk antibody matched group, significantly (chest muscle, lower limb flesh) or extremely significantly (spleen, fabricius bursa) be lighter than and do not treat matched group; Gaining effect is the most remarkable, total augment weight and all the weightening finish be higher than the yolk antibody matched group, the utmost point is significantly higher than does not treat matched group.The counteracting toxic substances prophylactic tria proves (table 4～table 9), and no matter singly we's usefulness still are and the same usefulness of vaccine all can reduce sick chicken death rate, improve specific antibody level.By the treatment checking of several groups of clinical disease chickens, protective rate all reaches about 90%.
Compared with prior art also have following advantage:
1. the Western medicine therapy has and uses dexamethasone and Norethisterone Compound treatment, but can only mitigation symptoms or mitigate the disease, can not reduce mortality rate.To early stage sick chicken, with high immunity yolk antibody or hyper-immune serum certain curative effect is arranged, but both all are vulnerable to condition effect such as preparation, storage and transport, cause the danger that effect is unstable or other disease is propagated in existence, and the injection trouble, also easily cause the stress of disease chicken.This medicine is made by pure Chinese medicine, and drug effect is remarkable, and is safe and reliable, can overcome above drawback.
2. this medicament is according to the traditional Chinese veterinary medicine theory, add heat-clearing and toxic substances removing, removing heat from blood YIN nourishing, blood-activating and stasis-removing with the Radix Astragali of QI invigorating and make, modern pharmacological research shows, the Radix Astragali can raise immunity, improve the immunosuppressant of disease chicken, heat and toxic materials clearing away medicine has antivirus action in its effective ingredient astragalus polysaccharides and the side, and drugs for nourishing yin can improve the dewatering state of sick chicken, and drug for invigorating blood circulation and eliminating stasis can improve blood circulation, it is hemorrhage to alleviate pathology, all medicines are harmonious, QI invigorating poison holding, treating both the principal and secondary aspects of a disease, and prescription is new reasonable.
3. most refusing to eat happiness is drunk behind the chickling morbidity chicken, and other similar Chinese medicines mostly are powder greatly, and by the administration of spice mixed feeding method, not only the absorption of medicine can not get ensureing, even also digest and assimilate process after taking in, drug effect can not in time be brought into play; This medicine, can guarantee the intake of medicine, and bring into play curative effect as early as possible by mixing the administration of drink method for extracting the oral liquid formulation of preparation.
4. this medicine has no side effect, and no drug residue meets the green medicine requirement, can ensure animal-derived food safety; Production process does not have the three wastes, environmentally safe.
The invention provides a kind of pure Chinese medicinal preparation of preventing and treating infectious bursal disease, evaluate by the new veterinary drug in Jiangsu Province, reached the standard of desired novelty, science and practicality in the patent.
Four, the specific embodiment
The recipe quantity medical material of the every 1000ml medicinal liquid of the blue antitoxin drink of stilbene is: Radix Astragali 300g, Radix Isatidis 200g, Folium Isatidis 200g, Radix Rehmanniae 200g, Radix Paeoniae Rubra 100g.
The technological process of producing every 1000ml medicinal liquid is: use water extraction.With above five kinds of Chinese medicine, decoct with water twice, each 1 hour, filtration, merging filtrate is concentrated into 1000ml, adds the 4g sodium benzoate, leaves standstill after stirring evenly, and precipitation is removed in filtration or centrifugal, the aseptic subpackaged bottle of going into to sterilize.
(1) the blue antitoxin drink test of pesticide effectiveness of stilbene
Counteracting toxic substances of the present invention treatment comparative test proves, with do not treat matched group, two prescription treatment groups of yolk antibody treatment matched group and other are compared, the curative effect of our processed group is best, pathology hemorrhage the lightest, gaining effect is the most remarkable.Counteracting toxic substances prevention comparative test proves, we no matter be single with or with the same usefulness of vaccine, all can reduce mortality rate, raising specificity antibody horizontal; By the treatment checking of several groups of clinical disease chickens, protective rate all reaches about 90%.
1. the blue antitoxin drink therapeutic test of stilbene
4 batches of young cock 1043 plumages (Nanjing hot spring chicken house provides) of the non-immune Luo Man egg of shared 21 ages in days of test of test method.Observe before the experiment and raise 48h, 35 wing venous blood collections are extracted in every batch of test at random out, after agar diffusion method detection IBDV maternal antibody feminine gender, are divided into 7 groups at random.The 7th group of 104 plumages, any processing is not done in isolated rearing; All the other 6 groups (the chicken number sees Table 1) take by weighing gross weight respectively, calculate counterpoise; Then with brush anus method counteracting toxic substances.Dispensing treatment during IBD classical symptoms such as behind the 24h, lethargy appears in the chicken group, the hair that spreads is beaten heap, watery diarrhea, fragmentary death.
I～III group, Chinese medicine experimental group, drink-service side 1, side 2, side 3 (the blue antitoxin drink of stilbene) successively; IV group, single principal agent matched group, the drink-service Radix Astragali, more than 4 groups dosage be the 1ml/ plumage, administration every day 1 time, 3d continuously; The V group, yolk antibody matched group, chest muscle injection yolk antibody 1ml/ plumage, 1 time; The VI group is not treated matched group.
Observation index and date processing (1) mortality rate: every day, death toll respectively organized in record, calculates mortality rate when 7d stops death to counteracting toxic substances.(2) organ hemorrhage positive rate: dead chicken is by only cuing open inspection, statistics chest muscle, lower limb flesh, spleen and the hemorrhage percentage rate that accounts for death toll of fabricius bursa.(3) weightening finish: observe stop death, clinical basic rehabilitation to the chicken group after, take by weighing each group survival chicken TBW respectively, calculate total augment weight and every plumage and all increase weight.More than the data of 3 indexs with each group of t check and the diversity of not treating matched group.
Result of the test (1) mortality rate (table 1): with the side is minimum for 3 groups, the secondly side of being 1, side 2, yolk antibody, single principal agent group, each group all with do not treat matched group (P＜0.05) or extremely remarkable (P＜0.01) difference arranged significantly.3 groups of sides are with the difference of not treating matched group the most remarkable (P＜0.005), greater than the yolk antibody group.The curative effect that shows the blue antitoxin drink of stilbene is best, is better than yolk antibody slightly, and the effect of compound recipe is better than the single principal agent.
(2) dead chicken organ hemorrhage positive rate (table 2): still minimum with 3 groups of sides, the hemorrhage percentage rate that accounts for death toll of chest muscle, lower limb flesh, spleen and fabricius bursa is respectively: 15.70%; 42.66%; 22.15%; 50.51%, with the significant difference of not treating matched group (P＜0.05, chest muscle, lower limb flesh) or extremely significantly (P＜0.01, spleen, fabricius bursa).The histoorgan that the side of showing 3 can significantly alleviate the disease chicken is hemorrhage, and effect is better than yolk antibody, single principal agent and other compound recipe.
(3) weightening finish changes (table 3): total augment weight is with 3 groups the highest of sides, the total augment weight 4.9kg 75.5g that all increases weight, and single principal agent matched group takes second place, and all the utmost point is significantly higher than and does not treat matched group (P＜0.001); All the other are respectively organized total augment weight and are negative.All still with 3 groups the highest of sides, single principal agent group is taken second place in weightening finish, and the utmost point is significantly higher than does not treat matched group (P＜0.001).The output capacity of the side of showing 3 is the highest, better economic benefit.
2. the blue antitoxin drink prophylactic tria of stilbene
Young cock 222 plumages of the non-immune Luo Man egg of test method 29 ages in days are divided into 5 groups at random.The I group, Chinese medicine (the blue antitoxin drink of stilbene) group, 37 plumages; The II group, vaccine group, 38 plumages; The III group, Chinese medicine adds 1 group of vaccine, 38 plumages; The IV group, Chinese medicine adds 2 groups of vaccines, 41 plumages; The V group, virus-positive matched group, 40 plumages; Other establishes blank group 28 plumages, and isolated rearing is done observation and had or not other infectious disease infection and normal utricule than usefulness.
Each group is weighed after observing raising 48h, the test 1 together of IBDV maternal antibody detection method.II～IV group vaccine eye dripping, collunarium immunity; III group is at vaccine immunity same day, I group and IV group 4d drink-service Chinese medicine before counteracting toxic substances, and dosage is every plumage 1g every day, continuously 4d; I～V group is 14d (45 age in days) brush anus counteracting toxic substances behind vaccine immunity, the average 0.2ml of every plumage; Behind the counteracting toxic substances same day and counteracting toxic substances 7,14,21,28,35d randomly draws the blood sampling of 10 plumages for every group, the monitoring antibody titer.
Observation index and date processing (1) mortality rate, cut open inspection and change: observed and recorded is with test 1.(2) rate of body weight gain: 35d takes by weighing survival chicken body weight behind the counteracting toxic substances, calculates the total augment weight rate of respectively organizing.Above data are checked with t, relatively the significance of difference between each treatment group and virus-positive matched group, Chinese herb on the prevention group and the vaccine group.(3) serum I BDV antibody titer monitoring method: use the AGP method.(4) capsule index: when the chicken that dies of illness is cutd open inspection, weigh in the balance simultaneously and get body weight, and separate fabricius bursa and weigh.And randomly draw chicken 3 plumages from the blank group and take by weighing its body weight and fabricius bursa weight.Press document then and calculate capsule exponential sum BBIX.Computing formula is as follows: B/W (capsule index)=fabricius bursa weight (g) ÷ body weight (g) * 1000; BBIX=test group capsule index ÷ blank group capsule index.If BBIX less than 0.7, then is judged to the fabricius bursa atrophy.
Result of the test (1) mortality rate: single I group mortality rate with Chinese medicine is minimum, compares significant difference (P＜0.05=with vaccine group and virus-positive matched group; The mortality rate that Chinese medicine adds III, the IV group of vaccine also is lower than vaccine group.Show that the blue antitoxin drink of stilbene has prevention IBDV effect, also be better than simple use vaccine (table 4) with the protective rate of vaccine use in conjunction.
(2) dead chicken organ hemorrhage positive rate: compare single lower limb flesh hemorrhage significantly (P＜0.05) minimizing that adds 1 group of vaccine with Chinese drug-treated group and Chinese medicine with vaccine group; The fabricius bursa of Chinese drug-treated group goes out significantly (P＜0.01) minimizing of bood exhaustion.Show that the blue antitoxin drink of stilbene can significantly alleviate lower limb flesh and the fabricius bursa hemorrhage (table 5) of disease chicken.
(3) weightening finish changes: the total augment weight variation is followed successively by IV group＞I group＞III group＞II group＞V group in proper order.Preceding 3 group utmost points are significantly higher than the II group, V organizes (P＜0.01).Show the blue antitoxin drink of stilbene single with or share all with vaccine and can obviously improve rate of body weight gain, acquisition favorable economic benefit (table 6).
(4) capsule index variation: 48h behind counteracting toxic substances, the BBIX that Chinese drug-treated group and Chinese medicine add 2 groups of vaccines is higher than 0.7, all the other times, the BBIX of each group all was lower than 0.7, it is described to press document, Chinese drug-treated group adds 1 group of vaccine at 72h～120h, vaccine group, Chinese medicine and adds 2 groups of vaccines at 72h～144h at 48h～120h, Chinese medicine, and fabricius bursa all is in atrophing state.Prompting has only the Chinese medicine of Chinese drug-treated group and the preceding 4d administration of counteracting toxic substances to add vaccine can resist the fabricius bursa atrophy for 2 groups in 48h, alleviate virogenetic damage (table 7).
Can find out also that from the result except that Chinese medicine added 2 groups of vaccines, the BBIX during all the other 3 groups of 120h raise during than 96h, shows that the damage of fabricius bursa has begun to recover, wherein the blue antitoxin drink of stilbene adds 1 group of rising amplitude of vaccine maximum, illustrates that the fabricius bursa injury recovery is the fastest.
(5) antibody changes: 1. antibody positive rate: 7d behind counteracting toxic substances, and Chinese drug-treated group is the highest, is 90%, and vaccine group is 62.5%, and Chinese medicine adds 1 group of vaccine and Chinese medicine, and to add 2 groups of vaccines be 87.5%; 14d is except that Chinese medicine adds 2 groups of vaccines, and all the other 3 groups are 100% (table 8).
2. antibody horizontal: the 7d Chinese drug-treated group is the highest behind counteracting toxic substances, is higher than 1 titre of vaccine group, and Chinese medicine adds 1 group of vaccine and Chinese medicine and adds 2 groups of vaccines and be higher than 0.88 titre of vaccine group.Chinese medicine adds 1 group of vaccine 21d behind counteracting toxic substances and reaches peak value, and all the other 4 groups behind counteracting toxic substances 28d just reached peak value; 35d behind counteracting toxic substances, the antibody horizontal of each group begin to descend, with the fastest (table 9) of Chinese medicine matched group decline.Above result shows that the speed of the blue antitoxin drink group antibody response of stilbene is fast, makes serum antibody reach a higher level in the shortest time.
(2) area validation
In the chickling of plumage 15～30 ages in days morbidity surplus totally 6 groups 5000 of poplar market town, Guanyun County priority, the classical symptom of infectious bursal disease all appears, and cut open inspection and be diagnosed as infectious bursal disease, after treating with the blue antitoxin drink of stilbene, protective rate is all about 90%.
(3) the blue antitoxin drink drug effect of stilbene is summed up
Prove through counteracting toxic substances treatment comparative test, in six kinds of different disposal groups, the curative effect of the blue antitoxin drink processed group of stilbene is best, pathology hemorrhage the lightest, gaining effect is the most remarkable.The counteracting toxic substances prophylactic tria proves that no matter singly we's usefulness still are and the same usefulness of vaccine all can reduce mortality rate, improve specific antibody level; By the treatment checking of several groups of clinical disease chickens, protective rate all reaches about 90%.
Death toll and mortality rate are respectively organized in table 1. counteracting toxic substances treatment experiment
| Index | Group | |||||
| I | II | III | IV | V | VI | |
| Sample number death toll mortality rate (%) | 117 23 19.67±5.51 B※ | 100 20 20.00±0 B | 195 37 18.97±3.31 C | 118 33 27.97±7.78 A | 195 41 21.03±8.96 B | 199 93 46.70±5.84 |
Annotate: with do not treat group and compare A, P＜0.05; B.P＜0.01; C, P＜0.005; D, P＜0.001.2 tables together down.
The organ hemorrhage positive rate (%) of dead chicken is respectively organized in table 2. counteracting toxic substances treatment experiment
| Organ | Group | |||||
| I | II | III | IV | V | VI | |
| Chest muscle lower limb flesh spleen fabricius bursa | 26.45±28.80 86.36±17.37 81.82±21.43 86.36±24.74 | 33.64±3.71 90.46±0.32 62.73±16.72 76.27±10.93 | 15.70 A±6.64 42.66 A±7.96 22.15 B±11.39 50.51 B±3.99 | 30.24±8.10 75.76±24.31 60.66±34.31 84.85±5.95 | 44.64±22.94 83.33±23.57 62.08±16.68 73.69±10.41 | 42.78±13.31 75.60±22.87 55.60±29.55 82.75±15.66 |
Administration front and back body weight and weightening finish are respectively organized in table 3. counteracting toxic substances treatment experiment
| Index | Group | |||||
| I | II | III | IV | V | VI | |
| Counterpoise (g) treatment back counterpoise (g) all increase weight (g) before gross weight (kg) treatment back gross weight (kg) total augment weight (kg) treatment before the treatment | 19.41 18.70 -0.71±0.70 C 165.9 198.9 33.0±36.3 | 20.73 20.23 -0.50±0.07 c 207.3 252.8 45.5±21.2 | 37.03 41.93 4.9±2.03 D 189.9 265.4 75.5±57.1 B | 19.46 20.01 0.55±0.63 D 164.9 235.4 70.5±1.6 A | 36.34 35.42 -0.92±1.40 C 186.3 230.0 43.7±20.5 | 36.86 24.31 -12.55±1.90 185.2 229.3 44.1±14.3 |
Death toll and mortality rate (%) are respectively organized in table 4. counteracting toxic substances prevention experiment
| Index | Group | ||||
| I | II | III | IV | V | |
| Sample number death toll mortality rate | 37 11 22.81 Aa | 38 17 44.74 | 38 13 34.21 | 41 15 36.59 | 40 19 47.50 |
Annotate: 1. compare A, P＜0.05 with the virus-positive matched group; B, P＜0.01; C, P＜0.005; D.P＜0.001.
2. compare a, P＜0.05 with vaccine group; B, P＜0.01; C, P＜0.05; D, P＜0.001.As follows.
The organ hemorrhage positive rate (%) of dead chicken is respectively organized in table 5. counteracting toxic substances prevention experiment
| Organ | Group | ||||
| I | II | III | IV | V | |
| Chest muscle lower limb flesh spleen fabricius bursa | 32.00 60.00 A 56.00 56.00 Bb | 36.36 84.85 54.55 87.88 | 23.33 56.67 A 76.67 83.33 | 33.33 75.76 63.64 90.91 | 45.16 70.97 58.06 83.87 |
Administration front and back body weight and total augment weight are respectively organized in table 6. counteracting toxic substances prevention experiment
| Index | Group | ||||
| I | II | III | IV | V | |
| Gross weight (kg) total augment weight (kg) total augment weight divides rate (%) in vain after preceding gross weight (kg) administration of administration | 11.73 51.95 40.22 342.88 Bb | 12.30 43.26 30.96 251.71 | 11.85 50.63 38.78 327.26 Bb | 12.25 58.83 46.58 380.24 Bb | 11.85 41.35 29.50 248.95 |
The fabricius bursa index variation is respectively organized in table 7. counteracting toxic substances prevention experiment
| Group | Time behind the counteracting toxic substances (h) | Index | |||
| Body weight (g) | Fabricius bursa heavy (g) | B/W | BBIX | ||
| I | 48 72 96 120 | 390.7±28.51 378.7±35.40 321.6±28.61 310.5±0 | 0.99±0.36 0.91±0.19 0.88±0.17 0.70±0 | 4.06 2.40 2.74 2.25 | 1.04 0.51 0.48 0.50 |
| II | 48 72 96 120 | 390.1 ± 48.74 384.5 ± 35.36 388.1 ± 22.71 337.3 ninth of the ten Heavenly Stems 20.91 | 0.84±0.17 0.85±0.28 1.07±0.22 0.95±0.06 | 2.15 2.20 2.75 2.82 | 0.61 0.47 0.48 0.63 |
| III | 48 72 96 120 | 389.8±27.93 372.8±50.46 337.3±16.56 318.3±29.86 | 0.65±0.20 0.88±0.19 0.70±0.10 0.95±0.26 | 1.67 2.36 2.08 2.98 | 0.47 0.50 0.36 0.67 |
| IV | 48 72 96 120 | 432.0±28.69 387.7±37.20 327.9±54.66 371.0±0 304.0±0 | 1.17±0.38 0.95±0.27 0.93±0.17 0.80±0 0.70±0 | 2.70 2.46 2.82 2.16 2.30 | 0.77 0.52 0.49 0.48 0.42 |
| V | 48 72 96 120 | 341.0±6.36 348.1±65.64 356.9±39.94 346.5±50.67 | 0.53±0.04 0.78±0.24 0.82±0.14 0.97±0.31 | 155 2.24 2.28 2.79 | 0.44 0.47 0.40 0.62 |
| Negative control group | 48 72 96 120 144 168 | 436.7±70.53 487.0±100.80 529.2±46.46 506.5±27.83 490.7±59.55 532.3±64.57 | 1.63±0.51 2.30±0.36 3.03±0.90 2.27±0.55 2.70±0.26 1.82±0.40 | 3.52 4.72 5.73 4.48 5.50 3.41 | - - - - - - |
Table 8 counteracting toxic substances prevention experiment is respectively organized the AGP antibody positive rate and is changed (%)
| Group | Time behind the counteracting toxic substances (d) | ||||
| 7 | 14 | 21 | 28 | 35 | |
| I II III IV V | 90 62.5 87.5 87.5 77.78 | 100 100 100 87.5 100 | 100 100 100 100 100 | 100 100 100 100 100 | 100 100 100 100 100 |
Table 9. counteracting toxic substances prevention experiment is respectively organized antibody titer and is changed (log 2)
| Group | Time behind the counteracting toxic substances (d) | ||||
| 7 | 14 | 21 | 28 | 35 | |
| I II III IV V | 2.50 1.50 2.38 2.38 2.22 | 8.50 8.20 8.50 6.25 8.63 | 9.70 8.80 10.13 8.50 8.75 | 10.7 11.00 9.75 9.88 10.25 | 8.56 1080 9.00 9.38 9.63 |
Claims (2)
1, a kind of herb mixture of preventing and treating infectious bursal disease, the medicinal liquid that it is characterized in that every 1000ml is to be made by following crude drug: Radix Astragali 300g, Radix Isatidis 200g, Folium Isatidis 200g, Radix Rehmanniae 200g, Radix Paeoniae Rubra 100g.
2. the described a kind of preparation method of preventing and treating the herb mixture of infectious bursal disease of claim 1 is characterized in that, with above five kinds of Chinese medicine, decoct with water twice, each 1 hour, filter merging filtrate, be concentrated into 1000ml, add 4 gram sodium benzoate, leave standstill filtration or centrifugal after stirring evenly, remove precipitation, the aseptic subpackaged bottle of going into to sterilize.
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| CN101332256B (en) * | 2007-06-28 | 2011-03-16 | 保定冀中药业有限公司 | Traditional Chinese medicine composition for infectious bursal disease and preparation method thereof |
| CN101690821B (en) * | 2009-10-21 | 2011-07-27 | 辽宁益康生物股份有限公司 | Immune protective agent of attenuated living vaccine against infectious bursal disease and preparation method thereof |
| CN111135251B (en) * | 2020-01-09 | 2021-11-26 | 太原市恩禾动物药业有限公司 | Veterinary drug for preventing and treating chicken bursal disease as well as preparation method and application thereof |
| CN116832096A (en) * | 2023-08-09 | 2023-10-03 | 南京农业大学 | Formula, preparation and application of traditional Chinese medicine emulsifiable paste for treating pet infection wound |
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2003
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