CN1253780A - Method for extracting active component of sea cockroach - Google Patents
Method for extracting active component of sea cockroach Download PDFInfo
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- CN1253780A CN1253780A CN99124016A CN99124016A CN1253780A CN 1253780 A CN1253780 A CN 1253780A CN 99124016 A CN99124016 A CN 99124016A CN 99124016 A CN99124016 A CN 99124016A CN 1253780 A CN1253780 A CN 1253780A
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- blatta seu
- extra large
- seu periplaneta
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Abstract
The method for extracting medicinal active component from sea roach includes the following steps: making pretreatment of sea roach, including natural air-drying, vacuum drying and pulverizing, then extracting by warm water, vacuum concentration, preservative treatment and decolouring by active carbon so as to obtain raw medicine filtrate 25-35g/ml. The animal tests show that the above-mentioned extract possesses obvious inflammation-relieving and pain-stopping action, can be used for preparing natural inflammation-relieving pain-stopping medicine or making functional food.
Description
The present invention relates to from the Blatta seu periplaneta of marine organisms sea, extract the method for medical active composition.
Marine organisms sea Blatta seu periplanetaes [ligia exotica (roux)] have another name called HAIQU, seashore beach louse, are a kind of longshore land and water amphibians of living in all the year round, belong to Crustachia, Ligiidae.Among the people once with extra large Blatta seu periplaneta as drug use.According to record, be the effect that principal agent has the infantile malnutrition of disappearing, invigorates blood circulation, detoxifies with extra large Blatta seu periplaneta, can control infantile malnutrition, tinea, traumatic injury, cellulitis from the beginning of (Yu Chuanlong, Huang Taitang " Chinese medicine Ci hai " volumes 2,1993 years) such as diseases.
The application among the people of extra large Blatta seu periplaneta is mainly contained dual mode: one, extra large Blatta seu periplaneta is dried with new watt, be ground into the end then, be used for oral, can reducing swelling and alleviating pain; Its two, extra large Blatta seu periplaneta is smashed to pieces, be used for external application, can control tinea and the detumescence.Above-mentioned application mode is very original, and its drug effect and active ingredient are further studied and confirmed.
The objective of the invention is to change the raw mode of application among the people sea Blatta seu periplaneta, a kind of method of extracting extra large Blatta seu periplaneta medicament active composition is proposed, so that improve the efficient of extra large Blatta seu periplaneta drug utilization, and open up new from the natural antiinflammation pain-stopping pharmaceutical of ocean or the channel of functional food.
The present invention is a raw material with marine organisms sea Blatta seu periplaneta, and extra large Blatta seu periplaneta polypide is flat, is avette, and is pitch-dark glossy, and the big person of body is golden yellow.The extracting method of the medicament active composition of sea Blatta seu periplaneta is as follows:
1. pretreatment: be about to extra large Blatta seu periplaneta natural air drying, vacuum drying, be ground into powder.
After the sea Blatta seu periplaneta catches, scald extremely, clean natural air drying with boiled water.Be dried to constant weight then in a vacuum.Pulverize again, cross 80 mesh sieves, get powder.
2. the extraction of active ingredient: with 30-40 ℃ of warm water extraction 2-3 time of extra large Blatta seu periplaneta powder, each 15-30 hour, merge extract just ,-10～-20 ℃ of cryogenic vacuums concentrate; Add activated carbon, in order to decolouring, reheat kept 3-5 minute to little boiling, and filter is proposed in cooling then, and sucking filtration liquid is regulated concentration to 25-35g crude drug/ml; Add antiseptic, transfer to etc. and to ooze; Adjust pH is to neutral; Packing, sterilization gets final product.
In the said extracted process, the antiseptic of adding can be parabens, as methyl hydroxybenzoate, ethyl hydroxybenzoate, propylparaben etc.Addition reaches 0.03～0.08% for making final concentration.
In the said extracted process, the amount that adds activated carbon reaches 0.3～0.5% for making final concentration.
Through zoopery, find that above-mentioned extra large Blatta seu periplaneta extract has significant anti-inflammatory analgetic effect, experimental technique and result are as follows:
One, experiment medicine:
1. extra large Blatta seu periplaneta extract: as above configuration, use for lumbar injection.
2. indometacin: Shanghai pharmaceuticals product, face with before, make 4mg/ml, use for lumbar injection.
Two, laboratory animal
Kunming mice, male, body weight 18 ± 2g is available from the The 2nd Army Medical College Experimental Animal Center.(regular grade, the certificate of competency: the moving word of doctor 02-25-1 number).
Three, experimental technique: with reference to the chief editor's of bureau of drug administration of Ministry of Health of the People's Republic of China " new drug preclinical study guideline compilation " mode.
2. mouse writhing experiment.
After the mice group, make negative control with normal saline, indometacin is made positive control, intraperitoneal injection, and after 40-60 minute, lumbar injection 0.6% acetic acid 0.2ml observed the mouse writhing number of times in 15 minutes thereafter.Significant difference between comparable group.
Illustrate: the mouse writhing reaction refers to the abdominal part indent that mice produces because of being subjected to pain stimulation, and trunk and back leg are upheld, the phenomenon of hips up.
2. the mice auricular concha causes scorching method
After the mice group, make negative control with normal saline, indometacin is made positive control, the drug injection lumbar injection.After the administration 30 minutes, dimethylbenzene: 0.03-0.05ml drips and is put on inside and outside the mouse right ear auricular concha.Put to death mice after 3 hours and take off the auris dextra shell, take off left auricular concha contrast simultaneously, after left and right ear was laid round auricle, weighing on analytical balance calculated the swelling degree to the card punch of 7mm respectively.(swelling degree=auris dextra auricular concha weight-left ear auricular concha weight).Significant difference between comparable group.
Four, experimental result
Table 1: extra large Blatta seu periplaneta extract Dichlorodiphenyl Acetate inducing mouse is turned round the inhibitory action of body
| Grouping | Number of animals | Dosage | Turn round the body number of times | Suppression ratio (%) |
| Normal saline | ??8 | ??16.1±6.6 | ||
| Indometacin | ??8 | ??60mg/kg×1 | ??1.2±1.4 ** | ?92.5 |
| The sea Blatta seu periplaneta extract | ??8 | ??0.5ml | ??0±0 ** | ?100 |
| ??8 | ??0.1ml | ??0.2±0.4 ** | ?98.8 | |
| ??8 | ??0.06ml | ??4.7±4.8 ** | ?70.8 |
* P＜005, * * P＜0.01 is compared with the normal saline group.
As known from Table 1, the inductive mouse writhing of extra large Blatta seu periplaneta Dichlorodiphenyl Acetate has significant inhibitory effect, and action intensity is relevant with dosage.2 dosage groups are better than indometacin.
The inhibitory action of the inductive mice auricular concha of the extra large Blatta seu periplaneta extract xylol of table 2 swelling
| Grouping | Number of animals | Dosage | Swelling degree (mg) | Suppression ratio (%) |
| Normal saline | ????5 | ?0.4±0.07 | ||
| Indometacin | ????6 | ???60mg/kg×1 | ?0.28±0.04 ** | ??30 |
| The sea Blatta seu periplaneta extract | ????6 | ???0.5ml | ?0.13±0.1 ** | ??67.5 |
| ????6 | ???0.1ml | ?0.28±0.08 ** | ??30 |
* P＜0.05, * * P＜0.01 is compared with the normal saline group.
As shown in Table 2, swelling has significant inhibitory effect to extra large Blatta seu periplaneta extract to the mice auricular concha, and inhibitory action increases with the increase of dosage.And action intensity is better than the indometacin group.
This experimental result shows that extra large Blatta seu periplaneta extract of the present invention has significant anti-inflammatory analgetic effect, and effect is better than indometacin.As seen have development and application as marine organisms and be worth, can be used to prepare new natural antiinflammation pain-stopping pharmaceutical or functional food from the ocean with pharmaceutically active.
Embodiment, the extra large Blatta seu periplaneta in that gather in area, Zhoushan, Chinese Zhejiang Province through aforementioned pretreatment, obtains extra large Blatta seu periplaneta powder.Take by weighing the 3g powder, extract 2 times down at 37 ℃ with water for injection, when carrying 24 hours at every turn, merge extractive liquid.At-15 ℃ of following vacuum concentration.It is 0.3% that the adding injection makes final concentration with activated carbon, is heated to little boiling, kept 4 minutes, and cooling then, sucking filtration, its sucking filtration liquid concentration is 30g sea Blatta seu periplaneta medicated powder/ml; Add the antiseptic propylparaben, transfer to etc. and to ooze.Add water for injection, adjust pH to 7.2, last packing, sterilization promptly get required extra large Blatta seu periplaneta active ingredient extract.The effect of the anti-inflammatory analgetic of this extract sees Table 1 and table 2.
Claims (3)
1. the extracting method of an extra large Blatta seu periplaneta active ingredient, with extra large Blatta seu periplaneta is raw material, through natural air drying, vacuum drying, pulverizing pre-treatment step, it is characterized in that 30-40 ℃ of warm water extraction 2-3 time of extra large Blatta seu periplaneta powder, each 15-30 hour, merge extract just, concentrate at-10～-20 ℃ of cryogenic vacuums; Add activated carbon, be heated to little boiling, kept 3-5 minute, cooling then, sucking filtration, sucking filtration liquid are regulated concentration to 25-35g crude drug/ml; Add antiseptic, transfer to and wait thoroughly; Adjust pH is to neutral, and packing is sterilized.
2. extra large Blatta seu periplaneta active ingredient extracting method according to claim 1 is characterized in that the antiseptic that adds is a parabens, and addition reaches 0.03～0.08% for making final concentration.
3. extra large Blatta seu periplaneta active ingredient extracting method according to claim 1 is characterized in that the addition of activated carbon reaches 0.3～0.5% for making final concentration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99124016A CN1089242C (en) | 1999-11-11 | 1999-11-11 | Method for extracting active component of sea cockroach |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99124016A CN1089242C (en) | 1999-11-11 | 1999-11-11 | Method for extracting active component of sea cockroach |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1253780A true CN1253780A (en) | 2000-05-24 |
| CN1089242C CN1089242C (en) | 2002-08-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN99124016A Expired - Fee Related CN1089242C (en) | 1999-11-11 | 1999-11-11 | Method for extracting active component of sea cockroach |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106937959A (en) * | 2017-05-03 | 2017-07-11 | 杭州医学院 | A kind of antitumor extra large Blatta seu periplaneta extract and its preparation technology and detection method and purposes |
| CN111214493A (en) * | 2020-03-03 | 2020-06-02 | 广东晟研新材料科技有限公司 | Analgesic liquid and preparation method thereof |
| CN111714519A (en) * | 2019-03-22 | 2020-09-29 | 中国科学院海洋研究所 | Application of organic solvent extract of sea cockroach and composition |
| CN115232228A (en) * | 2022-08-04 | 2022-10-25 | 中国科学院海洋研究所 | Method for extracting chitin from sea cockroach |
| CN115227723A (en) * | 2022-08-04 | 2022-10-25 | 中国科学院海洋研究所 | Preparation method and application of sea cockroach oligomer |
-
1999
- 1999-11-11 CN CN99124016A patent/CN1089242C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106937959A (en) * | 2017-05-03 | 2017-07-11 | 杭州医学院 | A kind of antitumor extra large Blatta seu periplaneta extract and its preparation technology and detection method and purposes |
| CN106937959B (en) * | 2017-05-03 | 2018-02-16 | 杭州医学院 | A kind of antitumor extra large Blatta seu periplaneta extract and its preparation technology and detection method and purposes |
| CN111714519A (en) * | 2019-03-22 | 2020-09-29 | 中国科学院海洋研究所 | Application of organic solvent extract of sea cockroach and composition |
| CN111714519B (en) * | 2019-03-22 | 2023-11-24 | 中国科学院海洋研究所 | Application and composition of organic solvent extract of sea cockroach |
| CN111214493A (en) * | 2020-03-03 | 2020-06-02 | 广东晟研新材料科技有限公司 | Analgesic liquid and preparation method thereof |
| CN115232228A (en) * | 2022-08-04 | 2022-10-25 | 中国科学院海洋研究所 | Method for extracting chitin from sea cockroach |
| CN115227723A (en) * | 2022-08-04 | 2022-10-25 | 中国科学院海洋研究所 | Preparation method and application of sea cockroach oligomer |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1089242C (en) | 2002-08-21 |
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