CN116369521A - A composition with intestinal health promoting effect - Google Patents
A composition with intestinal health promoting effect Download PDFInfo
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- CN116369521A CN116369521A CN202310231897.8A CN202310231897A CN116369521A CN 116369521 A CN116369521 A CN 116369521A CN 202310231897 A CN202310231897 A CN 202310231897A CN 116369521 A CN116369521 A CN 116369521A
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- Prior art keywords
- sesamin
- attapulgite
- essential oil
- ginger essential
- composition
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- 239000000203 mixture Substances 0.000 title claims abstract description 21
- 230000007413 intestinal health Effects 0.000 title claims abstract description 18
- 230000001737 promoting effect Effects 0.000 title claims abstract description 17
- PEYUIKBAABKQKQ-AFHBHXEDSA-N (+)-sesamin Chemical compound C1=C2OCOC2=CC([C@H]2OC[C@H]3[C@@H]2CO[C@@H]3C2=CC=C3OCOC3=C2)=C1 PEYUIKBAABKQKQ-AFHBHXEDSA-N 0.000 claims abstract description 63
- PEYUIKBAABKQKQ-UHFFFAOYSA-N epiasarinin Natural products C1=C2OCOC2=CC(C2OCC3C2COC3C2=CC=C3OCOC3=C2)=C1 PEYUIKBAABKQKQ-UHFFFAOYSA-N 0.000 claims abstract description 63
- VRMHCMWQHAXTOR-CMOCDZPBSA-N sesamin Natural products C1=C2OCOC2=CC([C@@H]2OC[C@@]3(C)[C@H](C=4C=C5OCOC5=CC=4)OC[C@]32C)=C1 VRMHCMWQHAXTOR-CMOCDZPBSA-N 0.000 claims abstract description 63
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/357—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having two or more oxygen atoms in the same ring, e.g. crown ethers, guanadrel
- A61K31/36—Compounds containing methylenedioxyphenyl groups, e.g. sesamin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/06—Aluminium, calcium or magnesium; Compounds thereof, e.g. clay
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/906—Zingiberaceae (Ginger family)
- A61K36/9068—Zingiber, e.g. garden ginger
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention provides a composition with an effect of promoting intestinal health, and belongs to the field of biological medicines. The composition is composed of sesamin powder, ginger essential oil and attapulgite powder, has the functions of improving intestinal dysfunction by the sesamin and ginger essential oil, protecting and repairing intestinal mucosa by the attapulgite, and has the functions of synergism and promotion of intestinal health. Pharmacological tests show that the sesamin powder, ginger essential oil and attapulgite powder composition has the functions of regulating intestinal functions, protecting intestinal mucosa, resisting bacteria and inflammation and promoting intestinal health; the combination of sesamin, ginger essential oil and attapulgite is obviously superior to single use or the combination of the sesamin, ginger essential oil and attapulgite, and has synergistic effect. Toxicological tests have shown that the compositions of the invention are safe to use.
Description
Technical Field
The invention belongs to the field of biological medicines, and particularly relates to a composition with an effect of promoting intestinal health.
Background
Intestinal function directly affects the digestive absorption of nutrients in food by humans and is important to maintain normal function of other organs of the human body. Diarrhea, gastrointestinal dysfunction, chronic inflammation, immune dysfunction and other intestinal dysfunction can cause pain to patients, and damage intestinal health and human health. At present, most of medicines for preventing and treating diarrhea and other intestinal dysfunction are antidiarrheal medicines, antibacterial and anti-inflammatory medicines, and have the problems of large side effect, easiness in generating drug resistance and the like. In the prior art, a novel oral preparation which is natural in origin, obvious in effect and free from obvious adverse reactions and is used for improving the intestinal functions and promoting the intestinal health is lacked, so that the pain of patients is relieved.
Sesamin is a main functional component in sesame seeds, and belongs to lignans; sesamin has various physiological activity functions of resisting oxidation, reducing blood lipid (serum cholesterol and triglyceride), protecting liver and kidney functions, etc., and can be applied to the fields of medicines and health care functional foods.
Ginger is a traditional health-care product resource with homology of medicine and food, and has multiple functions of invigorating stomach, warming stomach, dispelling cold, eliminating dampness, relaxing tendons, promoting blood circulation, resisting inflammation, resisting bacteria and the like. The ginger essential oil is volatile matters extracted from ginger rhizome, is brown transparent oily liquid, and has the pharmacological effects of activating blood circulation to dissipate blood stasis, resisting bacteria, resisting inflammation and the like; the ginger essential oil contains various functional substances such as gingerol, gingerol and the like, and is widely applied to the fields of food, health-care food, cosmetics and medicines.
Attapulgite is a clay mineral with a layered chain structure of hydrous magnesium aluminum silicate, has unique microstructure and charge property, has various excellent properties such as adsorptivity, colloid property and the like, and is widely applied to the fields of medicine, chemical industry, agriculture and environmental protection.
In the prior art, no report on the use of combination compatibility of sesamin, ginger essential oil and attapulgite for improving intestinal dysfunction and promoting intestinal health is found.
Disclosure of Invention
The invention aims to provide a composition with an effect of promoting intestinal health, which is a composition composed of sesamin powder, ginger essential oil and attapulgite powder, has the functions of improving intestinal dysfunction by the sesamin and the ginger essential oil, and protecting and repairing intestinal mucosa by the attapulgite, and has the effects of promoting intestinal health by synergistic interaction.
The mass ratio of sesamin to ginger essential oil to attapulgite powder is 0.1-2:0.1-2:10.
the sesamin is extracted from sesame seed meal, wherein the sesamin content is more than 50%; the ginger essential oil is extracted from ginger, wherein the gingerol content is more than 20%; the attapulgite powder is prepared by selecting attapulgite clay ore type with attapulgite crystal content of more than 50% and carbonate content of less than 10%, and grinding the attapulgite clay ore to 100-320 meshes. The composition can be processed into oral preparation such as granule, tablet, capsule, soft capsule, etc. by well known process.
Pharmacological tests show that the sesamin powder, ginger essential oil and attapulgite powder composition has the functions of regulating intestinal functions, protecting intestinal mucosa, resisting bacteria and inflammation and promoting intestinal health; the combination of sesamin, ginger essential oil and attapulgite is obviously superior to single use or the combination of the sesamin, ginger essential oil and attapulgite, and has synergistic effect. Toxicological tests have shown that the compositions of the invention are safe to use.
Detailed Description
The invention is further described below with reference to examples.
Example 1
A composition with intestinal health promoting effect comprises sesamin powder extracted from sesame seed cake, ginger essential oil extracted from rhizoma Zingiberis recens, and attapulgite powder, wherein the ratio of the sesamin powder to the ginger essential oil to the attapulgite powder is 1:1:10.
(1) Preparation of sesamin extract
The preparation method of sesamin extract comprises the following steps: grinding sesame seed meal into powder, adding n-hexane with the volume being 2 times of that of the sesame seed meal powder, stirring for 3 hours to degrease, filtering, degreasing for the 2 nd time by adopting the same method, filtering, and air-drying to obtain the degreased sesame seed meal. Adding 93% ethanol water solution into defatted sesame seed meal according to the proportion of adding 8.5ml 93% ethanol water solution into each gram of defatted sesame seed meal, stirring in a water bath at 55 ℃ for 2.5h, filtering, separating the extract and the defatted sesame seed meal, and repeatedly extracting the defatted sesame seed meal once by adopting the same method. Mixing the two extractive solutions, filtering, evaporating part of ethanol from the filtrate on rotary evaporator, concentrating under reduced pressure, and vacuum drying at 60deg.C to obtain sesamin extract with sesamin content of 698.07mg/g. The characters of the product are as follows: light brown powder, odorless, tasteless, hygroscopic, alcohol-soluble, and water-insoluble.
The sesamin content determination method comprises the following steps: HPLC was used. The sesamin standard substance solution is between 20 and 100 mug/ml, the corresponding peak area has obvious linear relation with the sesamin mass concentration, and a standard curve is prepared. Dissolving sesamin extract with chromatographic pure absolute ethanol, and filtering. Column temperature: room temperature; mobile phase: methanol-water (8:2, v/v); the flow rate is 0.8ml/min; sample injection amount: 20 μl; the detection wavelength is 290nm; analysis time: 15min. And (5) carrying out qualitative analysis according to the retention time of the standard sample, and calculating the sesamin content by an external standard method.
(2) Preparation of ginger essential oil
The preparation method of the ginger essential oil comprises the following steps: peeling rhizoma Zingiberis recens, cleaning, slicing, drying, and pulverizing to obtain rhizoma Zingiberis recens powder. Reflux-extracting rhizoma Zingiberis recens powder with 70% ethanol solution for 2 times, adding 70% ethanol solution 10 times of rhizoma Zingiberis recens powder, and extracting for 90min; adding 70% ethanol solution with volume 8 times of that of rhizoma Zingiberis recens powder into the second extraction, extracting for 60min, mixing ethanol extractive solutions, filtering, concentrating the filtrate under reduced pressure, and evaporating ethanol to obtain rhizoma Zingiberis recens essential oil. The characters of the product are as follows: pale yellow oily substance, has pungent taste, is soluble in alcohol, and is insoluble in water. The gingerol content in the ginger essential oil is 246.25mg/g.
The method for measuring the gingerol content comprises the following steps: adopting an HPLC method; dissolving rhizoma Zingiberis recens essential oil with chromatographic pure anhydrous ethanol, and filtering. Column temperature: room temperature; mobile phase: acetonitrile-methanol-water (40:5:55); the flow rate is 0.8ml/min; sample injection amount: 20 μl; the detection wavelength was 280nm. And (5) performing qualitative analysis according to the retention time of the standard sample, and calculating the content of gingerol by an external standard method.
(3) Activation of attapulgite
An attapulgite clay ore type having an attapulgite crystal content of greater than 50% and a carbonate content of less than 10% is selected. The attapulgite powder ground to 200 meshes is taken and added into a beaker filled with hydrochloric acid with the mass percentage concentration of 20%, a plastic film is sealed, and the mixture is magnetically stirred for 10 hours in a water bath at 60 ℃. Then, the supernatant was removed by centrifugation at 10000rpm, and the precipitate was washed with deionized water multiple times to give a supernatant having a neutral pH and a transparent color. Adding the washed precipitate into 10 percent (mass percentage concentration) sodium chloride aqueous solution, and performing ultrasonic treatment to form a suspension system; then magnetically stirring in a water bath at 60 ℃ for 10 hours; centrifuging at 10000rpm, collecting upper layer light gray solid, and drying at 70deg.C in electrothermal constant temperature air drying oven to constant weight to obtain activated attapulgite.
Example 2
In this example, animal experiments were performed using sesamin extract, ginger essential oil and activated attapulgite prepared in example 1 to examine the effects of sesamin extract, ginger essential oil and activated attapulgite in regulating intestinal function, protecting intestinal mucosa, resisting bacteria and inflammation, and promoting intestinal health. The sesamin used in this example was added as a sesamin extract, and the mass of the sesamin extract used was calculated based on the sesamin content of 698.07mg/g in the sesamin extract.
Preparation of E.coli bacterial suspension: inoculating Escherichia coli into LB medium, shake culturing at 37deg.C for 12 hr, centrifuging to obtain bacterial precipitate, washing bacterial precipitate with sterilized normal saline for 2 times, centrifuging, and making into 2×10 with sterilized normal saline 10 The CFU/mL E.coli suspension was stored at 4℃for further use.
Establishing a diarrhea model of an E.coli infected animal: c (C) 57 BL/6J mice were fasted and free-drinking water was performed 12 hours prior to the experiment. Each of whichE.coli liquid is injected into the abdominal cavity of the mice by 0.5mL, and when the mice have obvious diarrhea symptoms, watery stool, listlessness, slow action and other symptoms, the establishment of the diarrhea model of the animals infected by E.coli is successful; each mouse of the control group was intraperitoneally injected with an equal volume of sterilized normal saline.
And (3) test design: SPF class C6 weeks old 57 BL/6J mice 108 (male and female halves). All mice were acclimatized in a constant temperature and humidity SFP grade animal house for 3 days, controlled to 12h light/dark, with an ambient temperature of 25%, during which time the mice were free to eat and feed. After the adaptation, the mice were divided into 9 groups (12 groups each, each half of male and female) by a random method, which were respectively a normal control group, a model control group, and sesamin (ZMS, 60 mg. Kg) -1 ·d -1 ) Group ginger essential oil (GEO, 60 mg.kg) -1 ·d -1 ) Group, activated attapulgite (PGS, 60 mg.kg) -1 ·d -1 ) Group ZMS+GEO (30+30mg.kg) -1 ·d -1 ) Group ZMS+PGS (30+30mg.kg) -1 ·d -1 ) Group GEO+PGS (30+30mg.kg) -1 ·d -1 ) Group ZMS+GEO+PGS (20+20+20mg.kg) -1 ·d -1 ) A group. The administration was continued for 6 days.
The detection indexes comprise: the feed was weighed once a day and replaced and the average mouse feed intake was calculated. Mice body weight was recorded daily. The mice were observed for mental state, gastrointestinal tract response, motor and reactive capacity, etc. during the experiment. Diarrhea index: placing the mice into a squirrel cage paved with filter paper, and observing diarrhea condition of the mice; the diarrhea rate was calculated.
The results of the animal experiments are shown in tables 1-3 below.
TABLE 1 influence on mouse body weight
Note that: in Table 1, when a plurality of drugs are administered in a certain group, the numbers in the corresponding dose columns are added, wherein the positions of the numbers correspond to the drugs in the dose columns, for example ZMS+GEO group, and "30+30" in the dose columns indicates that sesamin 30 mg.kg.d. is administered -1 He Jiang (He Jiang)30 mg/kg/d essential oil -1 The same applies below; in comparison with the normal control group' * "P < 0.05" ** "means P < 0.01; in comparison with the model control group' # "P < 0.05" ## "means P < 0.01.
TABLE 2 influence on the food intake of mice
Note that: in Table 2, "compared with the normal control group" * "P < 0.05" ** "means P < 0.01; in comparison with the model control group' # "P < 0.05" ## "means P < 0.01.
TABLE 3 effect on diarrhea rate in mice
The results show that compared with the normal control group, the weight and the feeding amount of the mice in the model control group are obviously reduced, and the diarrhea rate is obviously increased. Compared with a model control group, the sesamin (ZMS), ginger Essential Oil (GEO) and attapulgite (PGS) intervention can obviously restore the weight and the food intake of diarrhea mice, reduce the diarrhea rate and promote the rehabilitation of the mice. The sesamin (ZMS), ginger Essential Oil (GEO) and attapulgite (PGS) have the effects of regulating intestinal functions, resisting bacteria and inflammation, protecting intestinal mucosa and promoting intestinal health; the combination of sesamin, ginger essential oil and attapulgite is obviously better than single use or the combination of the sesamin, ginger essential oil and attapulgite, and has synergistic effect. The above results show that the combination of sesamin, ginger essential oil and attapulgite has the effect of synergistically enhancing the drug effect.
The acute toxicity test result shows that the maximum dosage (MTD) of sesamin (ZMS) +ginger essential oil (GEO) +attapulgite (PGS) for gastric lavage of the mice is 22.9g/kg, which is equivalent to 70 times of the effective dosage; the animals did not show any adverse reactions. The product of the invention is safe and has almost no toxic or side effect.
Example 3
In this example, sesamin extract, ginger essential oil and activated attapulgite prepared in example 1 were used to prepare various preparations of drugs for promoting intestinal health. The sesamin used in this example was added as a sesamin extract, and the mass of the sesamin extract used was calculated based on the sesamin content of 698.07mg/g in the sesamin extract.
A tablet contains sesamin extract, ginger essential oil and activated attapulgite, each tablet contains 60mg sesamin, 120mg ginger essential oil and 600mg attapulgite. Mixing sesamin, ginger essential oil and attapulgite uniformly, and preparing tablets according to a conventional method.
A powder containing sesamin extract, ginger essential oil and activated attapulgite is provided, wherein each bag contains 120mg sesamin, 60mg ginger essential oil and 600mg attapulgite. Mixing sesamin, ginger essential oil and attapulgite uniformly, and preparing powder according to a conventional method.
A capsule contains sesamin extract, ginger essential oil and activated attapulgite, and each capsule contains 60mg sesamin, 30mg ginger essential oil and 600mg attapulgite. Mixing sesamin, ginger essential oil and attapulgite uniformly, and preparing capsules according to a conventional method.
The total mass of sesamin, ginger essential oil and attapulgite is about 700mg in terms of the effective part composition, and the oral dosage is used for regulating and improving intestinal functions and promoting intestinal health (such as intestinal dysfunction caused by chronic intestinal inflammation, functional dyspepsia, irritable bowel syndrome and the like), and the powder administration method is 2 times per day, 1 bag per time and warm boiled water for administration; the tablet is administered by 1-2 tablets each time, 2 times daily; the capsule is administered by 1-2 capsules each time, 2 times daily. 1 month is a treatment course.
Claims (6)
1. The composition with the effect of promoting intestinal health is characterized by comprising sesamin, ginger essential oil and attapulgite, wherein the mass ratio of sesamin to ginger essential oil to attapulgite is 0.1-2:0.1-2: 10-20.
2. The composition of claim 1, wherein the sesamin is extracted from sesame seed meal, wherein the sesamin content is greater than 50%.
3. Composition according to claim 1 or 2, wherein the ginger essential oil is extracted from ginger with a gingerol content of more than 20%.
4. A composition according to claim 3, wherein the attapulgite has a rod crystal content of greater than 50% and a carbonate content of less than 10%.
5. The composition of claim 4, wherein the attapulgite is ground to a powder of 100-320 mesh.
6. The composition of claim 5, wherein sesamin, ginger essential oil and attapulgite are mixed uniformly, and mixed with excipient to make powder, granule, tablet or capsule.
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