JPH09187248A - Antiallergic food - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an antiallergic food composed of a natural product, having safety, easily absorbed, effective in prophylaxis and treatment of allergosis such as atopic dermatitis and free from side effect by compounding a thyme extract and an oil and fat. SOLUTION: Seeds of thyme are crushed in a ball mile, the crushed material is charged into a Soxhlet extractor together with ether, and the solvent is heated to reflux for 20hr. The solvent is removed from the obtained extraction liquid under reduced pressure to dry up the extract. The obtained thyme extract is mixed and homogenized under heating on a hot water bath at 60 deg.C with an oil and fat such as perilla oil, docosahexaenoic acid(DHA) oil, borage oil or evening primrose oil to obtain the objective antiallergic food. The obtained food is composed of a natural product, accordingly safe and easily absorbed, effective in prophylaxis and treatment of allergosis such as atopic dermatitis and free from side effect.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an antiallergic food. More specifically, the present invention relates to an anti-allergic food containing thyme extract as an active ingredient and effective in preventing and treating allergic symptoms.

[0002]

2. Description of the Related Art In recent years, the number of patients with allergic symptoms tends to increase. Is it the direct cause of the increase in allergens contained in fungi, mites, pollen, etc., or is it due to an increase in biological reaction? Has not been elucidated. An allergic symptom occurs when an antigen-antibody reaction that occurs in the body against an allergen produces immunoglobulin E (IgE), and as a result, the cell membrane of mast cells is stimulated and histamine and leukotriene are released. These released substances have the action of promoting vascular permeability, the action of contracting smooth muscle, etc.,
Leukocytes and proteins leak from blood vessels and induce inflammation,
Bronchoconstriction causes asthma. Regarding the antiallergic component, a technique utilizing borneol's ability to stabilize mast cell membrane (Japanese Patent Laid-Open No. 6-2111713) and the anti-inflammatory action of actinomycete culture (Japanese Patent Laid-Open No. 5-25053) is known. On the other hand, it has been reported by Okuyama et al. That by ingesting α-linolenic acid in the form of oil and fat as a food, it has an effect of suppressing the production of leukotriene which causes an allergic reaction (Prostaglan).
din, Vol. 36, No. 3, 1988). Further, a technique utilizing the antiallergic properties of oils and fats containing docosahexaenoic acid (DHA) and α-linolenic acid (Japanese Patent Laid-Open No. 2-290).
No. 812) is known. However, although these natural antiallergic agents have few side effects, none of them are sufficiently effective. In order to reduce allergic symptoms, conventionally, antihistamines such as chlorpheniramine and diphenhydramine, mast cell stabilizers such as cromolyn and tranilast, and corticosteroids have been used orally or as an ointment. However, antihistamines have side effects such as drowsiness and dry mouth, and mast cell stabilizers cannot be expected to have the effect of reducing already occurring symptoms. Corticosteroids
It is accompanied by side effects such as gastrointestinal disorders, liver disorders, diabetes and high blood pressure. Moreover, all of these drugs are used for temporary treatment, and there is a problem that symptoms are reappeared when the drug is discontinued. Thyme is a dried leaf and bud of a Lamiaceae plant native to Spain, and is known as a highly antiseptic spice, and is widely used in meat dishes. As prior art utilizing the pharmacological action of thyme, insect repellents for clothes pests (JP-A-5-97618), whitening cosmetics (JP-A-6-199647), and skin external preparations (JP-A-7-76).
No. 61915 gazette) is known, but thyme's anti-allergic property has not been known.

[0003]

DISCLOSURE OF THE INVENTION In order to solve the above-mentioned problems of treatment with drugs, the present invention aims to provide an antiallergic food consisting of natural products, which is safe, easy to ingest, and highly effective. It was made as.

[0004]

As a result of intensive studies to solve the above problems, the present inventors have found that thyme extract is effective as an antiallergic food, and that thyme extract is used in combination with fats and oils. By doing so, it was found that a completely unexpected synergistic effect was developed, and the present invention was completed based on this finding. That is, the present invention provides (1)
An anti-allergic food characterized by containing a thyme extract, (2) an anti-allergic food characterized by containing a thyme extract and fats and oils, (3) the fat and oil is perilla oil (2). Anti-allergic food, (4) fat and oil is DH
The anti-allergic food according to item (2), which is oil A, (5) the anti-allergic food according to item (2), wherein the fat and oil is borage oil, and (6) the item (2), which is evening primrose oil. An anti-allergic food according to the above item is provided.

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION The antiallergic food of the present invention contains thyme extract as an active ingredient. Time
me) is a plant of the Labiatae family, Thymus serpyll.
um L. , Thymus vulgaris L .; , T
hymus purecox Opiz. , Thymu
s pannonocs L.S. , Thymus doe
rflert Ronn. , Thymus caesp
ittius Brot. It is a small shrub that has been cultivated as a raw material for spices since ancient times. The method for obtaining the thyme extract used in the present invention is not particularly limited, and any known extraction method can be used. For example, the dried thyme, sprouts, leaves, stems, roots, etc. of the raw material thyme are crushed using a ball mill or the like, and then water, ethanol, n
-Extract with a solvent such as hexane, ether, acetone,
The thyme extract can be obtained by removing the solvent by heating the extract or the like. Also, if necessary,
It can be further purified by chromatography or the like. The anti-allergic food of the present invention is not particularly limited in form as long as it can contain thyme extract, for example, a capsule containing thyme extract as it is, a fat suspension containing thyme extract. Capsules, powder obtained by mixing thyme extract and oil-absorbing starch, candy, drops, confectionery,
It can be chewing gum, gelatin capsule tablets, beverages and the like. The antiallergic food of the present invention containing a thyme extract can be easily ingested by forming a capsule tablet. In addition, thyme extract is mixed with glycerin fatty acid ester, propylene glycol fatty acid ester, sorbitan fatty acid ester, sucrose fatty acid ester, lecithin, glycerin, etc. in advance to obtain a uniform emulsified state, and these mixtures are generally used in foods. It can be mixed and dispersed in a specific raw material and processed into a desired form. In the antiallergic food of the present invention,
The content of the thyme extract is preferably 0.1 to 100% by weight. If the content of the thyme extract is less than 0.1% by weight, the antiallergic property may not be sufficiently exhibited.

In the antiallergic food of the present invention, the antiallergic property is exerted more strongly by combining thyme extract and fats and oils together. When the antiallergic food of the present invention contains oil and fat, the content of oil and fat is preferably 1 to 99% by weight, more preferably 10 to 50% by weight. When the content of the fats and oils is less than 1% by weight, the antiallergic enhancing effect may not be remarkably exhibited. On the other hand, when the content of fats and oils exceeds 99% by weight, the content of thyme extract becomes relatively small, and as a result, the antiallergic property may be lowered. There is no particular limitation on the fats and oils used in the present invention, for example, linseed oil, perilla oil, kaya oil, safflower oil, soybean oil and other drying oils, sesame oil, corn oil, rapeseed oil, cottonseed oil, lemon oil and other semi-oils. Non-drying oils such as drying oil, fennel oil, olive oil, kapok oil, camellia oil, peanut oil, cocoa butter,
Shea butter, palm oil, vegetable oil such as coconut oil, land animal oil such as beef butter, pork and horse fat, sardine oil, bonito oil, fish oil such as saury oil, herring oil and tuna oil, sharks, rays Examples thereof include liver oil, whale oil, sea animal oil such as dolphin oil, and the like. Among these fats and oils, perilla oil containing α-linolenic acid, perilla oil, borage oil containing γ-linolenic acid, evening primrose oil, docosahexaenoic acid (DH
A), purified fish oil containing eicosapentaenoic acid (EPA) and the like are preferable, and especially sesame oil containing α-linolenic acid,
DHA oil containing docosahexaenoic acid, borage oil containing γ-linolenic acid, and evening primrose oil containing γ-linolenic acid can be preferably used. All of these fats and oils are commercially available and can be easily obtained and used.

In the antiallergic food of the present invention, the combined use of thyme extract and sesame oil has a synergistic effect on the antiallergic properties of thyme extract and sesame oil, which is compared with the case where thyme extract is used alone. Then, an antiallergic effect equivalent to or higher than that is exhibited. When thyme extract and perilla oil are used in combination, the ratio is not particularly limited, but 10 to 40 wt% of thyme extract and perilla oil 90 can be used.
˜60% by weight, thyme extract 15
More preferably, the content is ˜30 wt% and the sesame oil is 85 to 70 wt%. Perilla oil
Is an oil containing linoleic acid and α-linolenic acid as main components, which is obtained from the seeds of the plant Lamiaceae Pellia ocymoides. The method of squeezing the sesame oil is not particularly limited, and it can be obtained, for example, by heating seeds, then crushing them and squeezing them. In the antiallergic food of the present invention, by adding a small amount of thyme extract to DHA oil, an antiallergic effect is strongly expressed, and an excellent antiallergic effect equivalent to or higher than the case where only thyme extract is used. Is obtained. When the thyme extract is added to DHA oil, the proportion thereof is not particularly limited, but it is preferable that the thyme extract is 0.1 to 20% by weight and the DHA oil is 99.9 to 80% by weight. More preferably, it is 0.5 to 10% by weight and DHA oil is 99.5 to 90% by weight. DHA oil is an oil and fat containing docosahexaenoic acid (DHA), which is an ω-3 fatty acid, and can be obtained from bonito oil, tuna oil, refined fish oil and the like.

In the antiallergic food of the present invention, by adding a small amount of thyme extract to borage oil, the antiallergic effect is strongly expressed, and it is as excellent as or better than the case where only thyme extract is used. Anti-allergic effect is obtained. When thyme extract is added to borage oil, the ratio is not particularly limited, but thyme extract 0.1%.
1 to 20 wt% and borage oil 99.9 to 80 wt% are preferable, and thyme extract 0.5 to 10 wt% and borage oil 99.5 to 90 wt% are more preferable. Borage oil is a plant of the family Musaceae Borago
officinalis L. Γ obtained from the seeds of
An oil that is rich in linolenic acid. After oiling, acid treatment,
A refined oil can be obtained through the steps of molecular distillation and deodorization. In the anti-allergic food of the present invention, by using thyme extract and evening primrose oil in combination, a synergistic effect appears in the anti-allergic properties of thyme extract and evening primrose oil, compared to the case of using thyme extract alone. An equivalent or higher anti-allergic effect is developed. When using thyme extract and evening primrose oil in combination, the ratio is not particularly limited,
The thyme extract is preferably 10 to 40% by weight and the evening primrose oil is 90 to 60% by weight, and more preferably the thyme extract is 15 to 30% by weight and the evening primrose oil is 85 to 70% by weight. Evening primrose oil is a plant of the family Acabbaceae Oenothe
It is an oil containing γ-linolenic acid obtained from the seeds of ratetraptera. The anti-allergic food of the present invention can be orally ingested as it is, but can be ingested as a capsule, tablet, emulsion or the like. The intake of the antiallergic food of the present invention is preferably 300 to 10,000 mg, more preferably 1,000 to 5,000 mg, and more preferably 2,000 to 3 as an amount of thyme extract per day for an adult. More preferably, it is 1,000 mg. Usually, adults take 2,000 per day
A sufficient effect is recognized at ~ 3,000 mg. The antiallergic food of the present invention is safe because it is composed of natural products, and is extremely easy to manufacture. Further, the anti-allergic food of the present invention can be naturally taken in a normal diet, especially when the thyme extract and fats and oils are used in combination, synergistically exhibit a remarkable inhibitory effect on allergic symptoms. Show.

[0009]

EXAMPLES The present invention will be described in more detail with reference to the following Examples, which should not be construed as limiting the present invention. Production Example 1 (Production of thyme extract) 1 kg of thyme was crushed with a ball mill to give ether 5.0
It was charged into a Soxhlet extractor together with 00 ml and heated under reflux for 20 hours. The obtained extract was dried under reduced pressure to obtain 45.3 g of a light brown thyme extract. Production Example 2 (Production of thyme extract) 1 kg of dried thyme leaves was crushed with a ball mill, 5,000 ml of methanol was added, and the mixture was left for 10 days. The obtained extract was dried under reduced pressure to give 38.3 g of dark green thyme extract.
I got Production Example 3 (Production of thyme extract) 1 kg of dried thyme leaf was crushed with a ball mill, placed in a Soxhlet extractor with 5,000 ml of ethanol, and heated under reflux for 20 hours. The obtained extract was dried under reduced pressure to obtain 43.2 g of a dark green thyme extract. Production Example 4 (Production of thyme extract) 1 kg of dried thyme leaves was ground with a ball mill, 10 liters of a 3 wt% sodium hydroxide aqueous solution was added, and the mixture was allowed to stand for 10 days. The pH of the extract obtained by filtration through Nutsche
Was adjusted to 7.0 with 1N hydrochloric acid and then freeze-dried under vacuum to obtain a brown thyme extract containing sodium chloride 5
11.9 g was obtained. Example 1 (capsule) Seamless capsule manufacturing machine [Freund Industrial Co., Ltd. product]
240 g of the thyme extract of Production Example 1 per
Capsules containing mg were prepared. Example 2 (capsule) Perilla oil [α-linolenic acid content 55% by weight: NOF
[Products] 80 parts by weight and 20 parts by weight of the thyme extract of Production Example 1 were mixed while heating to 60 ° C. in a hot water bath to homogenize to obtain an antiallergic food. Furthermore, a capsule containing 240 mg of the above-mentioned antiallergic food per grain was prepared using a seamless capsule manufacturing machine [Freund Industrial Co., Ltd.]. Example 3 (powder) 50 parts by weight of the thyme extract of Production Example 2 and 50 parts by weight of oil-absorbing starch [trade name Pineflow: Matsutani Chemical Co., Ltd. product] were mixed with a ribbon mixer to prepare a powder, and antiallergic. Got food. Example 4 (powder) 98 parts by weight of DHA oil [DHA content: 27% by weight: NOF Corporation product] and 2 parts by weight of the thyme extract of Production Example 2 were mixed while heating to 60 ° C. in a hot water bath. Later, Microfluidizer [Microfluid's product: homogenizing pressure 5
00kg / cm ² ] and homogenized using DH of thyme extract
An A oil suspension was obtained. Furthermore, DHA of this thyme extract
50 parts by weight of the suspension and 50 parts by weight of oil-absorbing starch [trade name Pineflow: product of Matsutani Chemical Co., Ltd.] were mixed with a ribbon mixer to prepare a powder to obtain an antiallergic food. Example 5 (Capsule) Borage oil [γ-linolenic acid content 25% by weight: NOF Corporation product] 99 parts by weight and 1 part by weight of the thyme extract of Production Example 3 were heated to 60 ° C. in a water bath. While mixing, homogenize to obtain a borage oil suspension of thyme extract. Furthermore, a capsule containing 240 mg of borage oil suspension of this thyme extract was prepared using a seamless capsule manufacturing machine [Freund Industrial Co., Ltd.]. Example 6 (powder) 90 parts by weight of DHA oil [DHA content 27% by weight: NOF Corporation product] and 10 parts by weight of the thyme extract of Production Example 4 were mixed while heating to 60 ° C. in a hot water bath. After that, Microfluidizer [Microfluid: Homogenization pressure 50
0 kg / cm ² ] and homogenized with DHA of thyme extract
A suspension was obtained. Further, 50 parts by weight of a DHA suspension of this thyme extract and 50 parts by weight of oil-absorbing starch [trade name Pineflow: a product of Matsutani Chemical Co., Ltd.] were mixed with a ribbon mixer to prepare a powder. Example 7 (capsule) Perilla oil [α-linolenic acid content 55% by weight: NOF
Products Co., Ltd.] 80 parts by weight, 15 parts by weight of the thyme extract of Production Example 3 and 5 parts by weight of carnauba wax were mixed while heating to 60 ° C. in a hot water bath, and then mixed with Microfluidizer [Microfluid Company: Homogenization pressure]. 500 kg / cm ² ] to obtain a suspension of thyme extract in perilla oil. Capsules containing 240 mg of the perilla oil suspension of this thyme extract were prepared using a seamless capsule manufacturing machine [Freund Industrial Co., Ltd.]. Comparative Example 1 (perilla oil-containing capsule) Seamless capsule manufacturing machine [Freund Industrial Co., Ltd. product]
Was used to prepare a capsule containing 240 mg of perilla oil [α-linolenic acid content 55% by weight: manufactured by NOF CORPORATION]. Comparative Example 2 (DHA oil-containing powder) 50 parts by weight of DHA oil [DHA content: 27% by weight: NOF Corporation product] and oil-absorbing starch [trade name: Pineflow: Matsutani Chemical Co., Ltd. product] Powder was prepared by mixing with a ribbon mixer to obtain a food product. Example 8 (Clinical test for allergic rhinitis) 30 patients (male 14) with allergic rhinitis (hay fever) patients
First name, 16 women, 22 to 43 years old), 10 people each in A group, B
The clinical trial was conducted for 2 months by dividing into 3 groups of a group and a group C. Patients in group A were administered capsules containing the thyme extract prepared in Example 1, patients in group B were administered capsules containing perilla oil prepared in comparative example 1, patients in group C were administered. Contains thyme extract 2 prepared in Example 2.
Capsules containing a mixture of 0% by weight and 80% by weight of sesame oil were administered. As a test method, 10 capsules were orally administered to the test subject every day after pollen symptoms appeared in the patient. On the other hand, daily activities such as eating were not limited. Diagnosis was performed 3 times in total, 2 weeks, 1 month and 2 months after the test start date. The effect was evaluated on the basis of the subjective symptoms of the test subject, and evaluated in four grades of excellent, effective, ineffective, and worse. In addition, the improvement rate was obtained by dividing the total number of patients who were markedly effective and effective by the number of subjects. Patients in group A were effective 2 after 2 weeks and ineffective 8 and had an improvement rate of 20% and effective 4 months later.
The improvement rate was 40% for 6 subjects and 6 invalid subjects, and the improvement rate was 80% for 4 subjects, 4 effective subjects and 2 invalid subjects after 2 months. After 2 weeks, patients in group B were effective 2 and ineffective 8 and the improvement rate was 20%, 1
After 3 months, the improvement rate was 30% in 3 effective patients and 7 ineffective patients, and the improvement rate was 40% in 4 effective patients and 6 ineffective patients after 2 months. After 2 weeks, the patients in group C were 4 effective and 6 ineffective, and the improvement rate was 40.
%, Improvement rate of 80% for 2 respondents, 6 effective and 2 invalid after 1 month, 100 improvement for 6 effective and 4 effective after 2 months
%Met. The results of the clinical trials are summarized in Table 1.

[0010]

[Table 1]

From the results shown in Table 1, the improvement rate of the group A patients who received the thyme extract was higher than that of the group B patients who received the sesame oil, and the thyme extract was more allergenic than the sesame oil. It was found that the rhinitis has a strong antiallergic property. Furthermore, unexpectedly, the improvement rate of the group C patients receiving the mixture of thyme extract and sesame oil was higher than that of the group A patients receiving only the thyme extract. The effect is shown early in patients with
Since many patients in the group showed remarkable effects, it was found that the combined use of thyme extract and perilla oil produced a remarkable synergistic effect. Example 9 (Clinical test for atopic dermatitis) 30 patients with atopic dermatitis (18 male, 12 female, 5)
(~ 32 years old) was divided into 3 groups of A group, B group and C group by 10 persons each, and a clinical study was conducted for 3 months. Patients in Group A were administered with the anti-allergic food containing the thyme extract prepared in Example 3, patients in Group B were administered with the food containing DHA oil prepared in Comparative Example 2, and Group C was administered. 2% by weight of thyme extract and DH prepared in Example 4
An antiallergic food containing a mixture of 98% by weight of oil A was administered. As the test method, 5 g of thyme extract, DHA oil or a food containing thyme extract and DHA oil was orally administered to each patient at a rate of 5 g each day. On the other hand, there were no particular restrictions on bathing and meals during the test. Diagnosis is 1 month after the start of the test, 2
The test was carried out three times after and three months later. Judgment of the effect
Based on the opinions of the subject and the tester, excellent, valid, invalid,
The evaluation was made in four stages of deterioration. In addition, the improvement rate was obtained by dividing the total number of patients who were markedly effective and effective by the number of subjects. After 1 month, patients in group A were 4 effective and 6 ineffective, and the improvement rate was 40.
%, Improvement rate 50% after 5 months with 5 effective and 5 ineffective 3
After 8 months, the improvement rate was 80% with 8 effective and 2 ineffective.
After 1 month, patients in group B had an improvement rate of 10% with 9 effective cases and an improvement rate of 20%, and 2 months later with 2 effective cases and 8 invalid cases showing an improvement rate of 20.
%, 3 months later, the effective rate was 4 and the invalid rate was 6 and the improvement rate was 40%. Patients in Group C had 4 effective and 6 ineffective after 1 month, 40% improvement rate after 2 months, 5 effective 50% after 2 months, 50% improvement rate after 3 months, 1 excellent response, 8 effective response, The improvement rate was 90% for one invalid person. The results of the clinical trials are summarized in Table 2.

[0012]

[Table 2]

From the results shown in Table 2, the improvement rate of the group A patients who received the thyme extract was higher than that of the group B patients who received the DHA oil.
It was found that the atopic dermatitis has a strong antiallergic property. Moreover, quite unexpectedly, patients in group A who received only thyme extract and 2% by weight of thyme extract.
Compared with patients in group C who received a mixture of 98% by weight and DHA oil, the improvement rate was similar or slightly higher in patients in group C, and adding a small amount of thyme extract to DHA oil was not effective. According to the above, it was found that an effect equivalent to or higher than the case where 100% thyme extract was used was exhibited. Example 10 (Anti-allergic test) 10 5-week-old mice (DDY: female) were divided into groups A and B, 5 mice each, and a powder feed [CE-
2, a product of CLEA Japan Co., Ltd.], mice of group B were allowed to freely ingest a mixture of 98% by weight of this powdered feed and 2% by weight of the thyme extract of Production Example 1 for 4 weeks. Three weeks later, 100 μl of a 0.5 wt% ethanol solution of dinitrophenylbenzene was applied to the abdomen of the mouse. In addition, 4
During the week, 20 μl of a 0.2% by weight solution of dinitrophenylbenzene in olive oil was applied to both ears of the mouse, and swelling of the earlobe caused by allergy was measured with a caliper after and 24 hours after the application, and the average value for each group was measured. And the standard deviation was calculated.

[0014]

[Table 3]

From the results shown in Table 3, the swelling of the earlobe of the mice ingested with the antiallergic food of the present invention was significantly smaller than that in the group fed with the powdered feed alone. It was confirmed that the product has sex. Example 11 (Anti-allergenicity test) Forty five-week-old mice (DDY: female), each including five mice, C
Group D, group E, group F, group G, group H, group I and group J, powdered feed [CE-2, CLEA Japan,
[Products Co., Ltd.]
% By weight and 5% by weight of the thyme extract of Preparation Example 3,
To the mice of group H, a mixture of 99% by weight of this powdered feed and 1% by weight of a suspension of thyme extract of Example 5 in borage oil,
For Group I mice, a mixture of 95% by weight of this powdered feed and 5% by weight of a DHA oil suspension of the thyme extract of Example 6 was used.
A group of mice was allowed to freely ingest a mixture of 90% by weight of this powdered feed and 10% by weight of a suspension of thyme extract of Example 7 in perilla oil for 4 weeks. The mice of groups E, F, and G were allowed to freely ingest the mixture of the powdered feed and the oil and fat shown in Table 4 for 4 weeks. In the third week,
100 μl of a 0.5 wt% ethanol solution of dinitrophenylbenzene was applied to the abdomen of the mouse. In addition, 4
During the week, 20 μl of a 0.2% by weight solution of dinitrophenylbenzene in olive oil was applied to both ears of the mouse. Immediately after and 24 hours after the application, the swelling of the earlobe caused by allergy was measured with a caliper, and the average for each group was measured. Values and standard deviations were determined. The swelling of the earlobe of the mice of group C not ingesting the thyme extract was 0.42 mm on average. Also,
The swelling of the earlobe of the mice of group D receiving the thyme extract was 0.29 mm on average. Group E, F who took oils and fats
The swelling of the earlobe of the mice of group G and group G was 0.3, respectively.
It was 2 mm, 0.27 mm and 0.28 mm. Further, the swelling of the earlobe of the mice of the H group, the I group and the J group, which ingested the mixture of the thyme extract and the fat and oil, was 0.15 mm and 0.1, respectively.
It was 6 mm and 0.16 mm. The average value and standard deviation of the administered feed, earlobe swelling are summarized in Table 4.

[0016]

[Table 4]

From the results shown in Table 4, the swelling of the earlobe of the mice of the group D receiving the thyme extract, the group E receiving the oil and fat,
The swelling of the ear lobes of the mice of the F group and the G group was significantly smaller than that of the mice of the C group receiving only the powdered feed, and thyme extract, borage oil, DHA oil, and perilla oil were all Was also confirmed to have anti-allergic properties. Furthermore, the swelling of the earlobe of the mice of the H group, the I group and the J group, which ingested the mixture of thyme extract and these oils and fats, was observed in the D group and the E group, which ingested only the thyme extract or the oils and fats.
It was found that the thyme extract and the oil / fat exert synergistic antiallergic properties, which are significantly smaller than the swelling of the earlobe of the mice of the F group, the F group and the G group. Example 12 (Measurement of anti-complement activity) Regarding thyme extract, mixture of thyme extract and fats and oils and fats and oils, a conventional method [Miyuki Shindo et al., Japanese and Chinese medicine, Volume 16, 76.
Page (1983)], the anti-complement activity was measured. As a comparative control, the measurement was performed using purified water and anti-allergic Chinese herbal medicine Saibokuto. In the test solution, the group A is pure water only, the group B is shibokuto, the group C is thyme extract, the group D is borage oil, the group E is DHA oil, the group F is sesame oil, and the group G is thyme extract. A mixture of 20% by weight of the product and 80% by weight of sesame oil, a group H a mixture of 10% by weight of the thyme extract and 90% by weight of borage oil, and a group I of a mixture of 1% by weight of the thyme extract and 99% by weight of DHA oil, Both are solutions in which 1% by weight is added to purified water and uniformly dispersed by ultrasonic waves. Veronal buffer p containing human serum and 0.15 mM calcium chloride, 0.5 mM magnesium chloride, 0.1% gelatin
50 μl each of H7.5 (GVB ⁺⁺ ) and the test solution were mixed and allowed to react at 37 ° C. for 30 minutes, and then GVB ⁺⁺
Was serially diluted with, sheep sensitized blood cells were added, and TCH5O was measured. Instead of the test solution, PBS ^- T when reacted with
CH5O was set to 100 and the decrease rate of TCH5O by the test solution was used as the anti-complement activity. The anti-complementary activity was 0.0% in the group A using pure water, 59.2% in the group B using Saiboku-to, and 54.4 in the group C using thyme extract.
1%. In addition, group D, group E and group F using borage oil, DHA oil and perilla oil were 36.3 each.
%, 35.0% and 30.3%. In addition, G group using sesame oil, H group using borage oil and I group using DHA oil together with thyme extract were 79.2%, 76.2% and 73.6%, respectively. . The composition and anti-complement activity of the samples used in the test are summarized in Table 5.

[0018]

[Table 5]

From the results shown in Table 5, it can be seen that the thyme extract has an antiallergic effect which is slightly lower than that of Saibokuto known as an antiallergic Chinese medicine. In addition, borage oil, DHA oil, and perilla oil each have considerably lower antiallergic properties than shibokuto. However, the combined use of these oils and fats with thyme extract increases the value of anti-complement activity,
It was found that the synergistic effect exerts excellent antiallergic properties.

[0020]

Although pollinosis is increasing in adults and atopic dermatitis is increasing in children, the preventive and therapeutic methods for these have not been established yet. The anti-allergic food containing the thyme extract of the present invention is safe because it is composed of natural products, and unlike conventional therapeutic methods based on anti-inflammatory agents, antihistamines, etc.,
It is possible to effectively prevent and treat anti-allergic symptoms without causing side effects.

Claims (6)

[Claims] 1. An anti-allergic food containing a thyme extract. 2. An anti-allergic food containing thyme extract and fats and oils. 3. The antiallergic food according to claim 2, wherein the fat or oil is perilla oil. 4. The antiallergic food according to claim 2, wherein the fat or oil is DHA oil. 5. The antiallergic food according to claim 2, wherein the fat or oil is borage oil. 6. The antiallergic food according to claim 2, wherein the oil and fat is evening primrose oil.