CN1251702C - Externally applied medicine for treating women's inflammation and its prepn process - Google Patents
Externally applied medicine for treating women's inflammation and its prepn process Download PDFInfo
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Abstract
The present invention relates to externally used medicine for curing women's inflammation and a preparation method thereof, which relates to a medicinal preparation of plant materials and a preparation method thereof. The medicine of the present invention is composed of active ingredients and/or auxiliary materials accepted by the medicine, the active ingredients mainly comprise supercritical carbon dioxide fluid extract, or alcohol extract or aqueous extract of plant medicine of the following parts by weight: 1 to 3 parts of snow lotus, 2 to 4 parts of lightyellow sophora root, 1 to 3 parts of fructus cnidii and 0 to 3 parts of belvedere fruit. The externally used medicine prepared by the preparation method of the present invention has the excellent functions of bacteria resistance, inflammation resistance and trichomonas resistance. The medicine containing the active ingredients extracted by the supercritical carbon dioxide has the best cure effect; on the aspect of bacteria resistance, the alcohol extract or the aqueous extract is weaker than the supercritical extract.
Description
Technical field
The present invention relates to a kind of gynecological inflammation external used medicine and preparation method thereof, relate to the medicinal preparation and the preparation method of the vegetable material of originating.
Background technology
Gynecological inflammation is common vaginitis, urethritis, cervical erosion, pruritus vulvae, leucorrhea abnormal etc., great majority cause owing to various antibacterials or fungal infection, external used medicine chemical synthetic drug commonly used at present is based on the antibiotics class, though these drug effects are fast, but many medicines can be touched absorption and human body is caused certain toxic and side effects by vagina is sticking, and the medicine that has also has certain carcinogenesis through zoopery.Antibiotics class medicine is easy to make vaginal microbial flora to change when being used for vaginitis, causes dysbacteriosis, and then brings out superinfection.
Chinese medicine is material base with the natural plants, and toxic and side effects is less, is the product of developmental research under instruction of Chinese Medicine theory, and its existing antibacterial and anti-inflammation functions can help to keep the normal microecological environment of vagina again, is beneficial to the elimination of inflammation.The middle medicament of relevant Herba Saussureae Involueratae also has exploitation in the gynecological at present, as the Herba Saussureae Involueratae capsule, the easypro the moon of Herba Saussureae Involueratae is clean etc., these all are made up of many flavor medical materials, adopt water to carry or alcohol extraction or directly be ground into powder and use, this must cause the utilization rate of active components of medical material low, the plant resources waste, and curative effect can not be satisfactory.
Summary of the invention
Still there is demand in the Chinese medicine for outer use that people better treat gynecological inflammation to curative effect.
The external Chinese drugs agentia that the purpose of this invention is to provide a kind of effective treatment gynecological inflammation.
Another object of the present invention provides the preparation method of this external Chinese drugs agentia.
For implementing the technical scheme that purpose of the present invention takes be: medicine of the present invention is made up of acceptable auxiliary on active component and the medicament, its active component mainly is the plant amedica by following weight portion, through the extract of supercritical carbon dioxide extraction or ethanol extraction or water extraction gained: 1～3 part of Herba Saussureae Involueratae, 2～4 parts of Radix Sophorae Flavescentiss, 1～3 part of Fructus Cnidii, 1～3 part of the Fructus Kochiae.
In above-mentioned weight portion scope, through a large amount of tests with grope to sum up and draw, all has curative effect preferably.Herba Saussureae Involueratae, heat-clearing and toxic substances removing, antiinflammatory; Radix Sophorae Flavescentis, heat clearing and damp drying; Fructus Cnidii, the outer kidney yang of temperature; The Fructus Kochiae, wind dispelling insecticide is antipruritic.Preliminary Study on extraction is found: use supercritical CO
2Extract Fructus Cnidii, Herba Saussureae Involueratae etc. and have advantages such as effective ingredient enrichment.Further pharmacodynamics proves: these extracts can keep or improve the pharmacologically active of these Chinese medicines, wherein Fructus Cnidii and Herba Saussureae Involueratae have complementarity aspect anti-white thought and the infusorian, on this basis, the Radix Sophorae Flavescentis and the Fructus Kochiae have been increased, the composition of this medicine is made monarch drug with Herba Saussureae Involueratae, Radix Sophorae Flavescentis is made ministerial drug, and Fructus Cnidii, the Fructus Kochiae are adjuvant drug.
Active component after preferred is the plant amedica by following weight portion, through the extract of supercritical carbon dioxide extraction gained: 2～2.5 parts of Herba Saussureae Involueratae, 2～3 parts of Radix Sophorae Flavescentiss, 1.5～2.5 parts of Fructus Cnidiis, the Fructus Kochiae 2～3; Best active component is the plant amedica by following weight portion, through the extract of supercritical carbon dioxide extraction gained: 2 parts of Herba Saussureae Involueratae, 3 parts of Radix Sophorae Flavescentiss, 2 parts of Fructus Cnidiis, 3 parts of the Fructus Kochiae.
When active component adopts the second ethanol extract, extract by the plant amedica of following weight portion:
1～2 part of Herba Saussureae Involueratae, 2～3 parts of Radix Sophorae Flavescentiss, 2～3 parts of Fructus Cnidiis, 1～2 part of the Fructus Kochiae.
When active component adopts water extract, extract by the plant amedica of following weight portion:
1～1.5 part of Herba Saussureae Involueratae, 3～4 parts of Radix Sophorae Flavescentiss, 1～3 part of Fructus Cnidii, 1～2 part of the Fructus Kochiae.
The preparation method of medicine of the present invention, it comprises the following steps: that (1) is the preparation of active component; (2) be on the active component adding medicine acceptable auxiliary routinely the technology of external medicine preparation make the receptible exterior-applied formulation of gynecological, it is characterized in that the preparation of active component: method a. is that Herba Saussureae Involueratae, Radix Sophorae Flavescentis, Fructus Cnidii, the Fructus Kochiae by described weight proportion put in the supercritical carbon dioxide extraction still and extracted, condition is: extraction temperature is 30-70 ℃, and extracting pressure is 20-35Mpa; The temperature of separating still is 35-70 ℃, and separating still pressure is 6-18Mpa; With ethanol, chloroform, acetone is entrainer, uses CO
2Cycling extraction 2-4 hour, after extraction finished, in the separating still discharge hole for discharge, extract obtained extractum after concentrating, and for supercritical carbon dioxide extraction thing density is 0.90～1.05g/ml (25 ℃), loss on drying is＜5%; Or method b. is that Herba Saussureae Involueratae, Radix Sophorae Flavescentis, Fructus Cnidii, the Fructus Kochiae by described weight proportion put in the Chinese medicine extraction pot, adds alcohol heating reflux twice, adds amount of alcohol at every turn and is not advisable to have powder, merges backflow twice, filters, and filtrate is condensed into extractum, is ethanol extract; Or method c. is that Herba Saussureae Involueratae, Radix Sophorae Flavescentis, Fructus Cnidii, the Fructus Kochiae by described weight proportion put in the Chinese medicine extraction pot, decocts with water twice, and each amount of water was not advisable to have powder, merged decocting liquid twice, filtered, and filtrate is condensed into extractum.
The preparation method a. of preferred active component is that Herba Saussureae Involueratae, Radix Sophorae Flavescentis, Fructus Cnidii, the Fructus Kochiae by described weight proportion put in the supercritical carbon dioxide extraction still and extracted, and extraction conditions is: extraction temperature is 30～70 ℃, and extracting pressure is 20～35Mpa; The separation reactor I temperature is 45～70 ℃, and separation reactor I pressure is 6～18Mpa; Separation reactor I I temperature is 35～60 ℃, and separation reactor I I pressure is 6～14Mpa; With ethanol, acetone, chloroform is entrainer, uses CO
2Cycling extraction 2-4 hour, after extraction finishes, respectively in separation reactor I, separation reactor I I discharge hole for discharge, extract is after concentrating, obtaining extractum is the supercritical carbon dioxide extraction thing, and extractum density is 0.90～1.05g/ml (25 ℃), and loss on drying is＜5%.
The preparation method a of best active component, the optimum condition of supercritical carbon dioxide extraction are that extraction temperature is 55 ℃, and extracting pressure is 30Mpa; The temperature of separation reactor I is 60 ℃, and separation reactor I pressure is 18Mpa; Separation reactor I I temperature is 40 ℃, and separation reactor I I pressure is 6Mpa; With 95% ethanol is entrainer, uses CO
2Cycling extraction 3 hours.Yield is 3.5%, and extractum density is 0.90～1.05g/ml (25 ℃), and loss on drying is＜5%.
Active component of the present invention can add conventional adjuvant required when preparing different dosage form, as glycerin gelatine commonly used, microcrystalline Cellulose etc. are made the receptible exterior-applied formulation of gynecological with the technology of the external medicine preparation of routine, as suppository, effervescent tablet, capsule, spray etc.
Suppository: adopt following proportioning raw materials, 1 part of the extractum of active component, 4 parts of glycerin gelatines.With glycerin gelatine heating for dissolving in water-bath, treat to dissolve fully evenly after, add extractum, stir, injection molding, cooling, packing promptly gets suppository.
Effervescent tablet: adopt following proportioning raw materials, 3～5 parts of the extractum of active component, 0.5 part of citric acid, 0.5 part of sodium bicarbonate, other excipient are an amount of, its preparation technology gets extractum, citric acid, other excipient of half amount approximately, makes acid particles A, drying with extractum as wetting agent, pellet moisture＜3%, get surplus extractum, 0.5 part of sodium bicarbonate, other excipient are made alkali grain B with extractum as wetting agent, drying, granule B moisture＜3%.With two kinds of granule mixings of A, B, tabletting behind adding lubricating substance, the fluidizer promptly gets effervescent tablet.
Capsule: adopt following proportioning raw materials, extractum 4-5 part of active component, with excipient such as microcrystalline Cellulose is made wetting agent with extractum, makes granule, and granule adds the lubricating substance mixing, and is promptly encapsulated.
The active component that obtains with preparation method provided by the invention carries out the test of pesticide effectiveness.
Test specimen: A, be supercritical CO
2Extract extractum, 1g extractum is equivalent to 34.4g crude drug amount.
B, be supercritical CO
2Extract extractum, 1g extractum is equivalent to 30.9g crude drug amount.
C, be supercritical CO
2Extract extractum, 1g extractum is equivalent to 32.8g crude drug amount.
D, for alcohol extraction extractum, 1g extractum is equivalent to 5.88g crude drug amount.
E, for alcohol extraction extractum, 1g extractum is equivalent to 6.70g crude drug amount.
F, be water extraction extractum, 1g extractum is equivalent to 4.10g crude drug amount.
G, be water extraction extractum, 1g extractum is equivalent to 4.35g crude drug amount.
Table sample A, B, C, D, E, F, G Chinese crude drug are formed
| Sample | Component | |||
| Herba Saussureae Involueratae (part) | Radix Sophorae Flavescentis (part) | Fructus Cnidii (part) | The Fructus Kochiae (part) | |
| A B C D E F G | 1 2 1.5 1 2 1 1.5 | 2 4 3 3 2 4 3 | 2 2 1.5 3 1 2 2 | 3 0 2 1 3 1 1.5 |
One, the vitro inhibition effect of trichomonas vaginitis is tested:
The method for making of infusorian culture medium (seeing " Human Parasite Science " second edition Zhao Weixian chief editor People's Health Publisher 1993)
Test method:
The preparation of infusorian liquid: getting that vagina that microscopic examination has the activity trichomonas vaginitis divides must thing, add in the above-mentioned culture fluid, (adding penicillin, each 1mg of streptomycin in every 10ml culture fluid) 37 ℃ of enrichment cultures 48 hours to suppress other bacterial growths, get culture fluid, count in microscopically with leukocytometer, it is made into that to contain borer population alive be 10 according to count results
5The suspension of individual/ml.
The mensuration of medicine vitro inhibition infusorian concentration: medicinal liquid 0.2ml is added 1.8ml culture medium (contain Chinese medicine 2560ug/ml, contain metronidazole 128ug/ml) doubling dilution.Adding concentration in medicinal liquid is 10
5Individual/ml infusorian liquid 0.1ml also makes blank and does not have the infusorian negative control of medicine with 2% dehydrated alcohol, cultivated 24 hours for 37 ℃, get culture fluid in the microscopically observed result, suppress the concentration of infusorian for it with the minimum drug level of no activity infusorian in the culture fluid.
Result of the test:
Table suppresses the lowest concentration of drug (ug/ml) of infusorian growth
Two, extracorporeal bacteria inhibitor test
During test by crude drug content with 50 ℃ the M-H agar culture medium that melts or sabouraud culture medium from 1000mg/ml carry out doubling dilution become 1000mg/ml, 500mg/ml, 125mg/ml, 62.5mg/ml, _ the serial pastille plate of ._ _ ._2.0mg/ml is stand-by.
Test strain: 18 strains of clinical separation pathogenic bacterium staphylococcus aureus, staphylococcus epidermidis 18 strains, escherichia coli 5 strains, Candida albicans 30 strains amount to 71 strains.
Standard Quality Control bacterial strain staphylococcus aureus ATCC25923, staphylococcus aureus 29213, escherichia coli ATCC25922
Test method: adopt the agar doubling dilution to measure the minimal inhibitory concentration (MIC value) of samples such as A-D.With multiple spot inoculation instrument with microbionation in the agar plate surface that contains different pharmaceutical concentration, every some bacteria containing amount is about 10
5CFU/ml was hatched 18 ~ 20 hours for 37 ℃, was the minimum inhibitory concentration (MIC value) of medicine to this bacterium with the least concentration of contained drug in the no bacterial growth plate culture medium.
Result of the test:
Vitro antibacterial activity (X ± SD) (mg/ml) of 7 kinds of (71 strain) bacterial strains of 7 Chinese medicines such as Table A-G
| Antibacterial (strain number) | MIC( X±SD) | ||||||
| A | B | C | D | E | F | G | |
| Staphylococcus aureus (18) | 0.5±0 | 0.55±0.35 | 0.75±0.65 | 15.6±6.01 | 15.6±8.13 | 25.21±11.76 | 51.25±21.91 |
| Table Portugal coccus (18) | 0.92±0.71 | 0.92±0.67 | 1.25±0.78 | 15.6±8.31 | 15.8±9.32 | 27.13±19.23 | 50.11±26.13 |
| Escherichia coli (5) | 21.8±13.6 | 25±0 | 50±0 | 125±0 | 125±17.34 | 250±76.13 | 250±81.01 |
| Candida albicans (30) | 0.5±0 | 0.5±0.32 | 1.0±0.57 | 12.9±37.1 | 57.98±68 | 98.32±106.1 | 79.62±81.95 |
Three, antiinflammatory test
Test reagent and animal: proinflammatory agent Oleum Tiglii is brown oil liquid, pharmaceutical grade, and lot number 000309 is produced by Jishui County, Jiangxi China precious natural medicinal oil factory, and with the preceding ethanol of using: water: it is standby that ether, 25: 2: 70 mixed solvents are mixed with 1% concentration.
Being diluted to concentration with 10% Tween 80 liquid before extractum uses is 80% liquid.
Healthy Kunming mouse, male, body weight 25-30 gram is provided feeding environment by Sichuan Industrial Institute of Antibiotics's animal center: temperature 21-22 ℃, humidity 56-65%.
Test method: each test will be with batch mice random packet, causing inflammation with 1% Oleum Tiglii makes and touches (30ul/ auris dextra), make to touch and on the swelling ear, smeared medication in back 1 hour, every ear is smeared 20ul sample liquid, make to touch and appended a drug in back 2 hours, make to touch and put to death animal in back 4 hours, get two ears, with residual medicinal residues on 37 ℃ of clean auricular conchas of normal saline rinsing, the filter paper suck dry moisture, reuse diameter 8mm card punch sweeps away two ear disks, and precision is weighed, about the difference of two auricle weight be swelling degree (mg), formula calculates swelling percentage rate (%) and inhibitory rate of intumesce (%) under the reuse:
Result of the test:
The extracorporeal anti-inflammatory effect of 7 Chinese medicines such as Table A-G
| Sample number | Number of animals (only) | Swelling degree (mg) | Swelling rate (%) | Inhibitory rate of intumesce (%) |
| A B C D E F G | 16 16 16 16 16 16 16 | 61.2±15.0 51.5±15.6 60.8±25.3 77.7±21.6 13.5±3.74 14.6±4.97 17.0±6.15 | 10.1±2.5 ** 9.1±2.8 ** 10.3±4.0 ** 12.6±2.8 0.87±0.23 0.93±0.32 1.12±0.42 | 35.9 42.0 38.7 19.7 17.5 9.1 1.4 |
Test of pesticide effectiveness result: compound saussurea involucrata sample A, B, C extractum all have good effect aspect antibiotic, antiinflammatory, the anti-trichomonal; Get sample product F, G extractum of alcohol extraction sample D, E extractum or water has good effect but effect is weaker than supercritical get sample product A, B, C at antibiosis.
Beneficial effect of the present invention: from above-mentioned evidence with the resulting external used medicine of the preparation method that the present invention carried all having good effect aspect antibiotic, antiinflammatory, the anti-trichomonal, be supercritical CO with active component
2The sample best results of extracting, alcohol extraction sample or the water product that get sample have good effect at antibiosis but act on the supercritical product that get sample that are weaker than.
Come further to set forth technical scheme of the present invention by the following examples.
Embodiment 1 takes by weighing Herba Saussureae Involueratae 1Kg, Radix Sophorae Flavescentis 4Kg, Fructus Cnidii 2Kg, Fructus Kochiae 1Kg put in the 5LHA-9805 type supercritical extraction reactor and extract, extraction kettle, separation reactor I, separation reactor I I, storage tank are heated respectively or cool off, when extraction kettle reaches temperature is 45 ℃, the separation reactor I temperature is 60 ℃, when separation reactor I I temperature is 40 ℃, open CO
2Steel cylinder pressurizes to system by high-pressure pump, and squeezing into an amount of ethanol is entrainer, and extraction kettle pressure reaches 35Mpa, and separation reactor I pressure is 14Mpa; When separation reactor I I pressure is 6Mpa, cycling extraction 2 hours, after extraction finishes, in separation reactor I, separation reactor I I discharge hole for discharge, extract concentrates through reduced vacuum drying (below the vacuum 0.1MPa, temperature is below 70 ℃) and obtains compound saussurea involucrata extractum 240g respectively, yield 3.0%, loss on drying 4.2%.
Embodiment 2 takes by weighing Herba Saussureae Involueratae 1.5Kg, Radix Sophorae Flavescentis 3Kg, Fructus Cnidii 2Kg, Fructus Kochiae 1.5Kg put in the Chinese medicine extraction pot, adding 10 times of water gagings decocted 2 hours, filter, medicinal residues add 5 times of water gagings again and decocted 1 hour, filter, merge twice and fry in shallow oil filtrate, the filtrate decompression vacuum drying is (below the vacuum 0.1MPa, temperature is below 70 ℃) be condensed into extractum 2.6Kg, extract dry weightlessness is 45%.
Embodiment 3, take by weighing Herba Saussureae Involueratae 2Kg, Radix Sophorae Flavescentis 2Kg, Fructus Cnidii 1Kg, Fructus Kochiae 3Kg, put in the Chinese medicine extraction pot, add 10 times of amount alcohol refluxs hour, filter, medicinal residues added alcohol reflux 1 hour again, filter, merge filtrate twice, filtrate decompression vacuum drying (below the vacuum 0.1MPa, temperature is below 70 ℃) is condensed into extractum, get 1.7kg, extract dry weightlessness is 46%.
Embodiment 4, take by weighing Herba Saussureae Involueratae 1.5Kg, Radix Sophorae Flavescentis 3Kg, Fructus Cnidii 1.5Kg, Fructus Kochiae 2Kg put in the 5LHA-9805 type supercritical extraction reactor and extract, extraction kettle, separation reactor I, separation reactor I I, storage tank are heated respectively or cool off, when extraction kettle reaches temperature is 45 ℃, the separation reactor I temperature is 60 ℃, separation reactor I I temperature is 40 ℃, the time, open CO
2Steel cylinder pressurizes to system by high-pressure pump, and squeezing into an amount of ethanol is entrainer, and extraction kettle pressure reaches 35Mpa, and separation reactor I pressure is 14Mpa; When separation reactor I I pressure is 6Mpa, cycling extraction 2 hours, after extraction finishes, in separation reactor I, separation reactor I I discharge hole for discharge, extract concentrates through reduced vacuum drying (below the vacuum 0.1MPa, temperature is below 70 ℃) and obtains compound saussurea involucrata extractum 233g respectively, yield 2.91%, loss on drying 3.2%.
Embodiment 5, take by weighing Herba Saussureae Involueratae 2Kg, Radix Sophorae Flavescentis 4Kg, Fructus Cnidii 2Kg puts in the 5LHA-9805 type supercritical extraction reactor and extracts, extraction kettle, separation reactor I, separation reactor I I, storage tank are heated respectively or cool off, when extraction kettle reaches temperature is 45 ℃, the separation reactor I temperature is 60 ℃, separation reactor I I temperature is 40 ℃, the time, open CO
2Steel cylinder pressurizes to system by high-pressure pump, and squeezing into an amount of ethanol is entrainer, and extraction kettle pressure reaches 35Mpa, and separation reactor I pressure is 14Mpa; When separation reactor I I pressure is 6Mpa, cycling extraction 2 hours, after extraction finishes, in separation reactor I, separation reactor I I discharge hole for discharge, extract concentrates through reduced vacuum drying (below the vacuum 0.1MPa, temperature is below 70 ℃) and obtains compound saussurea involucrata extractum 237g respectively, yield 2.96%, loss on drying 2.94%.
The preparation of the suppository of medicine of the present invention
With glycerin gelatine 2Kg heating for dissolving in water-bath, treat to dissolve fully evenly after, add extractum 500g, stir, injection molding, cooling, the demoulding, packing promptly gets suppository, makes 1000, every contains extractum 500mg.
The preparation of the effervescent tablet of medicine of the present invention
Get 200g extractum, citric acid 180g microcrystalline Cellulose 100g pregelatinized Starch 200g makes granule A with extractum as wetting agent, in 60 ℃ of dryings, and granule A moisture＜3%.
Other gets residue 300g extractum, and sodium bicarbonate 100g dibastic sodium phosphate 250g microcrystalline Cellulose 50g pregelatinized Starch 100g makes granule B with extractum as wetting agent, in 60 ℃ of dryings, and granule B moisture＜3%.With two kinds of granules of A, B and gas phase silica gel mix homogeneously, add magnesium stearate again, mixed 5 minutes, last machine tabletting promptly gets effervescent tablet.The tabletting condition is a temperature below 20 ℃, and relative humidity is below 50%.Make 1000, every contains extractum 500mg
The preparation of the capsule of medicine of the present invention:
Get compound saussurea involucrata extractum 200g microcrystalline Cellulose 50g lactose 100g pregelatinized Starch 139g, mix homogeneously is made wetting agent with ethanol, makes granule, and is dry under 60 ℃, pellet moisture＜3%.20 order granulate add an amount of magnesium stearate, gas phase silica gel, mix homogeneously, and last machine is encapsulated, makes 1000, and every contains extractum 200mg.
Claims (6)
1, a kind of gynecological inflammation external used medicine, it is made up of acceptable auxiliary on active component and the medicament, it is characterized in that described active component is the plant amedica by following weight portion, through the extract of supercritical carbon dioxide extraction or ethanol extraction or water extraction gained:
1～3 part of Herba Saussureae Involueratae, 2～4 parts of Radix Sophorae Flavescentiss, 1～3 part of Fructus Cnidii, 1～3 part of the Fructus Kochiae.
2, according to the said external used medicine of claim 1, it is characterized in that active component is the plant amedica by following weight portion, through the extract of supercritical carbon dioxide extraction gained:
2～2.5 parts of Herba Saussureae Involueratae, 2～3 parts of Radix Sophorae Flavescentiss, 1.5～2.5 parts of Fructus Cnidiis, 2～3 parts of the Fructus Kochiae.
3, according to the said external used medicine of claim 1, it is characterized in that active component is the plant amedica by following weight portion, through the extract of supercritical carbon dioxide extraction gained:
2 parts of Herba Saussureae Involueratae, 3 parts of Radix Sophorae Flavescentiss, 2 parts of Fructus Cnidiis, 3 parts of the Fructus Kochiae.
4, according to the said external used medicine of claim 1, it is characterized in that active component mainly is the plant amedica by following weight portion, through the extract of ethanol extraction gained:
1～2 part of Herba Saussureae Involueratae, 2～3 parts of Radix Sophorae Flavescentiss, 2～3 parts of Fructus Cnidiis, 1～2 part of the Fructus Kochiae.
5, according to the said external used medicine of claim 1, it is characterized in that active component is the plant amedica by following weight portion, through the extract of water extraction gained:
1～1.5 part of Herba Saussureae Involueratae, 3～4 parts of Radix Sophorae Flavescentiss, 1～3 part of Fructus Cnidii, 1～2 part of the Fructus Kochiae.
6, a kind of preparation is as the method for the said external used medicine of claim 1, and it comprises the following steps: that (1) is the preparation of active component; (2) be on the active component adding medicine acceptable auxiliary routinely the technology of external medicine preparation make the receptible exterior-applied formulation of gynecological, it is characterized in that the preparation of active component: method a. is that Herba Saussureae Involueratae, Radix Sophorae Flavescentis, Fructus Cnidii, the Fructus Kochiae by described weight portion put in the supercritical carbon dioxide extraction still and extracted, condition is: extraction temperature is 45 ℃, and extracting pressure is 35Mpa; The temperature of separating still is 40-60 ℃, and separating still pressure is 6-14Mpa; With ethanol is entrainer, uses CO
2Cycling extraction 2 hours, after extraction finished, in the separating still discharge hole for discharge, extract obtained extractum after concentrating, and for the survey 25 ℃ time of supercritical carbon dioxide extraction thing density is 0.90～1.05g/ml, loss on drying is＜5%; Or method b. is that Herba Saussureae Involueratae, Radix Sophorae Flavescentis, Fructus Cnidii, the Fructus Kochiae by described weight portion put in the Chinese medicine extraction pot, adds alcohol heating reflux twice, adds amount of alcohol at every turn and is not advisable to have powder, merges backflow twice, filters, and filtrate is condensed into extractum, is ethanol extract; Or method c. is that Herba Saussureae Involueratae, Radix Sophorae Flavescentis, Fructus Cnidii, the Fructus Kochiae by described weight portion put in the Chinese medicine extraction pot, decocts with water twice, and each amount of water was not advisable to have powder, merged decocting liquid twice, filtered, and filtrate is condensed into extractum.
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| CN103041007B (en) * | 2012-09-28 | 2015-04-22 | 陈政秀 | Externally-applied composite medicament for treating gynecologic inflammation |
| CN105727172A (en) * | 2016-02-16 | 2016-07-06 | 周燕燕 | Medicinal composition for treating pelvic inflammatory disease and preparation method thereof |
| CN107714845A (en) * | 2017-11-30 | 2018-02-23 | 康凤岐 | A kind of pharmaceutical composition and its preparation and purposes |
| CN108938784A (en) * | 2018-09-14 | 2018-12-07 | 高军起 | A kind of medicated pad improving gynecological disease |
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