CN1240276C - Embedding material biological sample and method for making specimen of human body - Google Patents
Embedding material biological sample and method for making specimen of human body Download PDFInfo
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- CN1240276C CN1240276C CN 03133280 CN03133280A CN1240276C CN 1240276 C CN1240276 C CN 1240276C CN 03133280 CN03133280 CN 03133280 CN 03133280 A CN03133280 A CN 03133280A CN 1240276 C CN1240276 C CN 1240276C
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Abstract
The present invention relates to an embedding agent for embedding biological specimens and a method for using the embedding agent for manufacturing a human body specimen, namely an embedding agent for biological specimens and a method for manufacturing a human body specimen. In order to solve the technical problem of poorer transparence of specimens manufactured by the existing embedding agents for biological specimens, the present invention adopts the following technical scheme that the embedding agent for biological specimens comprises the following components by portion in volume: 1 portion of epoxy resin, 0.01 to 0.05 portion of curing agent and 0.1 to 0.2 portion of diluting agent, wherein the epoxy resin is epoxy resin E40. In order to solve the technical problem that the embedding layer and the biological specimen of a specimen manufactured by the existing manufacture methods for biological specimens are separated from each other, the present invention adopts the following steps of slicing, cleaning, washing, dewatering, soaking, molding, embedding and demoulding. The present invention is mainly used for manufacturing biological specimens and human body specimens, the embedding layer has the advantages of no color, good transparence, etc., and the phenomenon that an embedding layer and a human body specimen are separated from each other is avoided.
Description
Technical field:
The present invention relates to a kind of method that biological sample is carried out the embedding medium of embedding and uses this embedding medium making human body specimen.
Background technology:
At present, mainly be to adopt traditional moist preservation method to the store method of biological sample, be about to biological sample and be immersed in the preservative and deposit.Relatively Chang Yong preservative is a formaldehyde, at first formaldehyde is mixed with 10% the aqueous solution, then biological sample is immersed in the formalin.Because formaldehyde has stronger penetrating odor, breathing, digestion, nerve and vision system to human body have severe impairment, and carcinogenesis arranged, reveal the damage human body in order to prevent formaldehyde, the biological sample that adopts formalin to make must be left in the container of sealing in use, it is bigger to take up room, carrying, mobile all inconvenient.In the process of preparation formalin and making biological sample, for avoiding contacting formaldehyde, the operator generally will take safeguard procedures, operates cumbersome equally.Owing to the easy polymerization reaction take place of formaldehyde precipitates, need often to change formalin, higher to the cost of biological sample long preservation.In addition,, changed the color of the original quality of sample with the biological sample that formaldehyde is made, second-rate.
In order to overcome the shortcoming that moist preservation method exists, an application for a patent for invention that is called " packaging method for making human-body specimen ", application number were disclosed on Gazette of Patent for Invention on December 22nd, 1993: 93106329.0, publication number: CN1079606A.The general steps that human body specimen is made in this patent application is, at first adopt alcohol and glacial acetic acid that human body specimen is dewatered, to be placed in the mould through the human body specimen of dehydration again, adopt the unsaturated polyester resin cast at last, after unsaturated polyester resin solidifies, human body specimen is embedded in wherein, at last product is taken out from mould, carry out grinding and buffing and handle.It is little, in light weight that the human body specimen that adopts this method to make has a volume, deposits, the advantage of carrying, conveniently moving.But also there is following shortcoming in the human body specimen that adopts this method to make: one, encapsulating layer presents yellow color in various degree, and transparency is relatively poor, is unfavorable for observer soma, influences its use value; Two, separative phenomenon between encapsulating layer and the human body specimen forms steam fog, easily so that do not see sample between encapsulating layer and the human body specimen; Three, because embedding medium can not be penetrated in the tissue, make between encapsulating layer and the human body specimen and produce segregation phenomenon, in order to guarantee the intensity of encapsulating layer, must increase the thickness of encapsulating layer, increased the usage amount of embedding medium, manufacturing cost is risen, weight increases the weight of; Four, owing to mould is not adopted specially treated, the outer surface of the encapsulating layer of sample is coarse, need carry out grinding and buffing to outer surface, complex process, and cost increases.
Summary of the invention:
One of the technical problem to be solved in the present invention is that the sample transparency that prior biological sample embedding medium is made is relatively poor.For solving this technical problem, the present invention by the following technical solutions: the imbedding biological specimen agent, form by epoxy resin, curing agent and thinner; Its special character is: epoxy resin adopts epoxy resin E40, and the volumetric ratio of epoxy resin, curing agent and thinner is:
The epoxy curing agent thinner
1 0.01～0.05 0.1～0.2
Curing agent of the present invention can adopt polysulfide rubber.
The elasticity of the consumption decision embedding medium of polysulfide rubber can increase and decrease according to concrete requirement.
Curing agent of the present invention can also adopt the ATU addition product.
Thinner of the present invention adopts a kind of in single epoxy radicals, bis-epoxy base and the three epoxy radicals reactive diluents.
What of thinner consumption are influential to the color of embedding medium, added the color jaundice, should select in above-mentioned amount ranges.
Thinner of the present invention can adopt n-butyl glycidyl ether.
N-butyl glycidyl ether belongs to single epoxy radicals reactive diluent.
Thinner of the present invention can also adopt allyl glycidyl ether.
Allyl glycidyl ether belongs to bis-epoxy base reactive diluent.
Two of the technical problem to be solved in the present invention is that the sample that adopts prior biological preparation of specimen method to make produces segregation phenomenon between encapsulating layer and the biological sample.For solving this technical problem, the present invention by the following technical solutions: use above-mentioned imbedding biological specimen agent to make the method for human body specimen, its special character is to adopt following steps:
A. section:
Treated human body corpse is sawn into certain thickness section;
B. clear up, wash:
With the content in the section removal tube chamber that obtains among the step a, and rinse well;
C. dehydration:
Successively adopt alcohol and acetone that the section of handling through step b is dewatered;
D. soak:
Adopt glycerine to soak the section of handling through step c;
E. adorn mould:
To pack in the mould through the section that steps d was handled;
F. embedding:
Add thinner in epoxy resin, add curing agent again, stirring just forms embedding medium, embedding medium is filled in the mould of step e, embedding medium will be cut into slices cover fully;
G. the demoulding:
After embedding medium among the step f solidifies, remove mould, take out cured block.
In step a, generally at first to handle the human body corpse, for example: anticorrosion fixedly processing, preserved skin, cleaning, freezing etc., will be slit into certain thickness section then through freezing autopsy saw, two sections of section generally also will be planished.The thickness of section is generally 5～10mm.
In step b, generally the appearance of the section after thawing to be rinsed well, the tube chamber content is cleaned out.
In step c, adopt dehydration of alcohol and adopt the acetone dehydration to carry out respectively according to sequencing.Directly adopt the alcohol (for example absolute alcohol) of high concentration although the dehydration dehydrating effect is good, cause section to shrink easily, influence the quality of human body specimen.Employing gradually to the dehydration of alcohol in high concentration gradient, both can reach the effect of dehydration by low-concentration ethanol, can prevent the section contraction again.If directly adopt the acetone dehydration without dehydration of alcohol, though the effect of acetone dehydration is better, cause section to shrink equally easily, influence the quality of human body specimen.Because the price of acetone is more expensive, at first adopts dehydration of alcohol, adopt the acetone dehydration then, can reduce the consumption of acetone, reduce the cost of making human body specimen.Because acetone not only has dehydration, and there are degreasing, the present invention to adopt the main purpose of acetone dehydration to be,, help combining of in the step of back embedding medium and tissue, embedding medium is penetrated in the tissue through the section of acetone dehydration.
In steps d, after section was soaked through glycerine, because glycerine combines with embedding medium easily, glycerine can guide embedding medium to be penetrated in the tissue in the step of back, and embedding medium and human body specimen are become one.
In step e, the size of mould, shape and structure are suitable, guarantee that the thickness of embedding medium can not be too thick, and the perfusion embedding medium is convenient, and the demoulding is easy, and it is bright and clean that embedding medium solidifies the back outer surface.
In step f, embedding medium adopts the above-mentioned embedding medium of being made up of epoxy resin, curing agent and thinner, epoxy resin adopts epoxy resin E40, curing agent can be selected above-mentioned polysulfide rubber, ATU addition product etc. as required, and thinner can be selected a kind of in above-mentioned single epoxy radicals, bis-epoxy base and the three epoxy radicals reactive diluents as required.The concrete ratio of epoxy resin, curing agent and thinner should be selected in the aforementioned proportion scope.
In the dehydration of the step c of technique scheme of the present invention, at first, will cut into slices and successively immerse 65～75%, 75～85%, 85～95%, carry out the gradient dehydration in 95～absolute alcohol, the time of every grade of dehydration is 2～4 days; Then, will successively immerse in the acetone for three times through the section after the dehydration of alcohol gradient and dewater, the time of each dehydration is 2～4 days.
In the steps d of technique scheme of the present invention, will cut into slices to immerse in the glycerine and soak 3～7 days.
Among the step e of technique scheme of the present invention, mould adopts two safety glass and one to have certain flexible sealing strip, and the width of sealing strip is slightly larger than the thickness of section; Stick mylar respectively on the surface of each sheet safety glass, to be placed on through the section that steps d was handled between two surfaces of posting mylar of two safety glass, two safety glass are parallel to each other, adopt the sealing strip sealing around between two safety glass, sealing strip is trapped among section wherein, leave certain space between sealing strip and the section, the two ends of sealing strip stay an osculum as perfusing hole.
Because embedding medium will discharge a large amount of heats in solidification process, the temperature of mould is raise, because safety glass has resistance and high temperature resistance property, can prevent that die deformation from damaging.Adopting safety glass is not unique a kind of selection of the present invention, adopts the sheet material of other material to replace safety glass, if satisfy high temperature resistant, have requirements such as enough intensity, any surface finish, all in protection scope of the present invention.The main effect of mylar is to be convenient to the embedding medium curing and demolding, and mylar has resistance and high temperature resistance property equally, can prevent that the heat that is discharged in the embedding medium solidification process from damaging.Adopt the mylar neither unique a kind of selection of the present invention, adopt the film of other material to replace mylar, as long as satisfy requirement such as high temperature resistant, any surface finish, all in protection scope of the present invention.Sealing strip can adopt plastic flexible pipe, and it has certain elasticity, has resistance and high temperature resistance property again.The diameter of the outer surface of plastic flexible pipe is exactly the width of foregoing sealing strip, and this diameter is generally than the big 2～4mm of thickness that cuts into slices.The general clamp with suitable chucking power that adopts makes two safety glass that sealing strip is compressed on the outer surface of two safety glass.Because sealing strip has certain elasticity, thereby sealing strip will be sealed between two safety glass.Two sections of section respectively and the distance between the inner surface of two safety glass (being the thickness of encapsulating layer) by the control of the width of sealing strip, sealing strip is compressed the back by two safety glass certain elastic deformation takes place, and the distance of section between the inner surface of safety glass of section is controlled at about 1mm.
Compare with No. 93106329.0 patent applications, the biological sample that adopts imbedding biological specimen agent of the present invention to make, advantage such as that its encapsulating layer has is colourless, transparency is good is convenient to observe biological sample outward appearance and tissue, has improved the use value of biological sample.Compare with No. 93106329.0 patent applications, the method that the present invention makes human body specimen can make embedding medium be penetrated in the tissue, on the one hand, make embedding medium and human body specimen one integrated mass, prevented the phenomenon that encapsulating layer is separated with human body specimen, avoid between encapsulating layer and human body specimen, producing steam fog, be convenient to observer soma, improved the quality of human body specimen; On the other hand, can reduce the thickness of encapsulating layer, reduce the consumption of embedding medium, reduce cost of manufacture.Because the present invention makes the method for human body specimen and adopts special mould, make the outer surface up and down of encapsulating layer of human body specimen of making bright and clean, do not need that grinding and buffing is carried out on these two surfaces and handle, compare with No. 93106329.0 patent applications, technology of the present invention is simple, and manufacturing cost reduces.
Description of drawings:
Fig. 1 makes the structural representation of mould in the method for human body specimen for the present invention;
Fig. 2 is the enlarged drawing of the cross section of mould among Fig. 1.
Embodiment:
Embodiments of the present invention is described in detail below in conjunction with accompanying drawing.
Embodiment 1:
Present embodiment is a kind of imbedding biological specimen agent, and it comprises epoxy resin, curing agent and thinner.Wherein, epoxy resin adopts epoxy resin E40, and curing agent adopts polysulfide rubber, and thinner adopts n-butyl glycidyl ether.The volumetric ratio of epoxy resin, curing agent and thinner is:
The epoxy curing agent thinner
1 0.01 0.1
Embodiment 2:
Present embodiment is a kind of imbedding biological specimen agent, and it comprises epoxy resin, curing agent and thinner.Wherein, epoxy resin adopts epoxy resin E40, and curing agent adopts the ATU addition product, and thinner adopts allyl glycidyl ether.The volumetric ratio of epoxy resin, curing agent and thinner is:
The epoxy curing agent thinner
1 0.01 0.1
Embodiment 3:
Present embodiment is a kind of method that adopts the imbedding biological specimen agent to make human body specimen, and this method adopts following steps:
A. section:
At first adopt formalin to carry out anticorrosion fixedly processing,, again corpse is rinsed well through anticorrosion fixing processing back whole body preserved skin to the human body corpse; Freezing below-25 ℃ temperature to corpse then, after the deep colling, corpse is sawn into the section of desired thickness; At last, with two section planings of section, the thickness that makes section is 10mm;
B. clear up, wash:
The section that obtains among the step a is thawed, remove the content in the tube chambers such as enteric cavity, blood vessel, and rinse well;
C. dehydration:
At first, the section that step b was handled successively immerses 70%, 80%, 90%, carry out 4 grades of gradients dehydrations in the absolute alcohol, and the time of every grade of dehydration is 3 days; Then, will successively immerse in the acetone for three times through the section after the dehydration of alcohol gradient and dewater, the time of each dehydration is 3 days;
D. soak:
Adopt glycerine to soak the section of handling through step c, soak time is 5 days;
E. adorn mould:
As depicted in figs. 1 and 2, mould comprises safety glass 1, safety glass 2 and a sealing strip 3.Sealing strip 3 adopts has certain flexible plastic flexible pipe, about the thickness big 3mm of the diameter of the outer surface of this plastic flexible pipe than section.At first on a surface of safety glass 1, stick mylar 4, on a surface of safety glass 2, stick mylar 5.Secondly, to be placed on surface and the safety glass 2 that safety glass 1 posts mylar 4 through the section 6 that steps d was handled posts between the surface of mylar 5, two safety glass are parallel to each other, adopt sealing strip 3 sealings around between two safety glass, sealing strip 3 will be cut into slices and 6 will be trapped among wherein, leave certain space between sealing strip 3 and the section 6, the perfusing hole 7 of an osculum as embedding medium stayed at the two ends of sealing strip 3.At last, on the outer surface of safety glass 1 and safety glass 2, adopt clip 8 to clamp, two safety glass are compressed sealing strip 3.Sealing strip 3 is compressed the back by two safety glass certain elastic deformation takes place, and the distance that two section branches of section 6 are taken leave of between the inner surface of two safety glass is controlled at about 1mm.
F. embedding:
At first dispose the imbedding biological specimen agent, this embedding medium comprises epoxy resin, curing agent and thinner.Wherein, epoxy resin adopts epoxy resin E40, and curing agent adopts polysulfide rubber, and thinner adopts n-butyl glycidyl ether.The volumetric ratio of epoxy resin, curing agent and thinner is:
The epoxy curing agent thinner
1 0.01 0.1
According to aforementioned proportion, in epoxy resin, add thinner, add curing agent again, stirring just forms embedding medium, by perfusing hole 7 embedding medium is filled in the mould of step e, embedding medium will be cut into slices 6 cover fully, with perfusing hole 7 sealings;
G. the demoulding:
Embedding medium among the step f is opened clip 8 after solidifying, and removes safety glass 1, safety glass 2, sealing strip 3, mylar 4 and mylar 5, takes out cured block.
Cured block is brighter and cleaner with mylar 4 contacts formation with mylar 5 two surface ratios, does not need grinding and buffing, contacts the periphery that forms with sealing strip 3 and need be polished into square or other shape, carries out polishing then.
By the human body specimen that above-mentioned steps is made, its encapsulating layer water white transparency, embedding medium is penetrated in the tissue, makes encapsulating layer and tissue one integrated mass.
Claims (10)
1. imbedding biological specimen agent is made up of epoxy resin, curing agent and thinner; It is characterized in that: epoxy resin adopts epoxy resin E40, and the volumetric ratio of epoxy resin, curing agent and thinner is:
The epoxy curing agent thinner
1 0.01～0.05 0.1～0.2
2. imbedding biological specimen agent according to claim 1 is characterized in that: curing agent adopts polysulfide rubber.
3. imbedding biological specimen agent according to claim 1 is characterized in that: curing agent adopts the ATU addition product.
4. according to claim 1,2 or 3 described imbedding biological specimen agent, it is characterized in that: thinner adopts a kind of in single epoxy radicals, bis-epoxy base and the three epoxy radicals reactive diluents.
5. imbedding biological specimen agent according to claim 4 is characterized in that: thinner adopts n-butyl glycidyl ether.
6. imbedding biological specimen agent according to claim 4 is characterized in that: thinner adopts allyl glycidyl ether.
7. the method for using claim 1,2,3,4,5 or 6 described imbedding biological specimen agent to make human body specimen is characterized in that, adopts following steps:
A. section:
Treated human body corpse is sawn into certain thickness section;
B. clear up, wash:
With the content in the section removal tube chamber that obtains among the step a, and rinse well:
C. dehydration:
Successively adopt alcohol and acetone that the section of handling through step b is dewatered;
D. soak:
Adopt glycerine to soak the section of handling through step c;
E. adorn mould:
To pack in the mould through the section that steps d was handled;
F. embedding:
Add thinner in epoxy resin, add curing agent again, stirring just forms embedding medium, embedding medium is filled in the mould of step e, embedding medium will be cut into slices cover fully;
G. the demoulding:
After embedding medium among the step f solidifies, remove mould, take out cured block.
8. method according to claim 7 is characterized in that: in the dehydration of step c, at first, will cut into slices and successively immerse 65～75%, 75～85%, 85～95%, carry out the gradient dehydration in 95～absolute alcohol, the time of every grade of dehydration is 2～4 days; Then, will successively immerse in the acetone for three times through the section after the dehydration of alcohol gradient and dewater, the time of each dehydration is 2～4 days.
9. method according to claim 7 is characterized in that: in the steps d, will cut into slices to immerse in the glycerine and soak 3～7 days.
10. method according to claim 7 is characterized in that: among the step e, mould adopts two safety glass and one to have certain flexible sealing strip, and the width of sealing strip is slightly larger than the thickness of section; Stick mylar respectively on the surface of each sheet safety glass, to be placed on through the section that steps d was handled between two surfaces of posting mylar of two safety glass, two safety glass are parallel to each other, adopt the sealing strip sealing around between two safety glass, sealing strip is trapped among section wherein, leave certain space between sealing strip and the section, the two ends of sealing strip stay an osculum as perfusing hole.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 03133280 CN1240276C (en) | 2003-07-28 | 2003-07-28 | Embedding material biological sample and method for making specimen of human body |
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| CN 03133280 CN1240276C (en) | 2003-07-28 | 2003-07-28 | Embedding material biological sample and method for making specimen of human body |
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| CN1480038A CN1480038A (en) | 2004-03-10 |
| CN1240276C true CN1240276C (en) | 2006-02-08 |
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2003
- 2003-07-28 CN CN 03133280 patent/CN1240276C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107136051A (en) * | 2017-07-17 | 2017-09-08 | 广州越城丰茂农林科技有限公司 | A kind of preparation method of amber sample |
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| CN1480038A (en) | 2004-03-10 |
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