CN112825846A - Specimen display box and specimen display method - Google Patents
Specimen display box and specimen display method Download PDFInfo
- Publication number
- CN112825846A CN112825846A CN201911166260.5A CN201911166260A CN112825846A CN 112825846 A CN112825846 A CN 112825846A CN 201911166260 A CN201911166260 A CN 201911166260A CN 112825846 A CN112825846 A CN 112825846A
- Authority
- CN
- China
- Prior art keywords
- specimen
- sample
- accommodating
- solution
- receiving member
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
Abstract
The application relates to a specimen display box, include: a first receiving member; the second accommodating piece and the first accommodating piece form an accommodating cavity; a specimen holder provided in the first receiving member or the second receiving member; and the liquid injection hole is arranged in at least one of the first accommodating part and the second accommodating part. Adopt the sample show box that this disclosed embodiment provided to demonstrate the sample, improved the transparency to the sample show, solved the opaque problem of transparent sample solid seal in epoxy. The application also provides a specimen display method.
Description
Technical Field
The present application relates to the field of biological specimen display technologies, and for example, to a specimen display box and a specimen display method.
Background
A small animal transparent specimen is a specimen which is used for treating an animal body and enabling muscles of the small animal to become transparent by using a chemical reagent so as to show stained animal bones. The transparent specimen can directly show the shape and position of the skeleton of an animal body, and has higher scientific research and teaching values. Meanwhile, the transparent specimen is very beautiful and has certain market value.
At present, the specimen is displayed by adopting a method of fixedly sealing the specimen in epoxy resin.
In the process of implementing the embodiments of the present disclosure, it is found that at least the following problems exist in the related art: the specimen is sealed in the epoxy resin, so that the transparency of the specimen is influenced.
Disclosure of Invention
The following presents a simplified summary in order to provide a basic understanding of some aspects of the disclosed embodiments. This summary is not an extensive overview nor is intended to identify key/critical elements or to delineate the scope of such embodiments but rather as a prelude to the more detailed description that is presented later.
The embodiment of the disclosure provides a specimen display box and a specimen display method, which aim to solve the problem that the transparency of a specimen is influenced by fixedly sealing the specimen in epoxy resin.
In some embodiments, the specimen display cartridge comprises: a first receiving member; the second accommodating piece and the first accommodating piece form an accommodating cavity; a specimen holder provided in the first receiving member or the second receiving member; and the liquid injection hole is arranged in at least one of the first accommodating part and the second accommodating part.
In some embodiments, the specimen presentation method comprises: fixing a specimen to be displayed on a specimen fixing piece of the specimen display box; connecting a first accommodating piece and a second accommodating piece of the specimen display box to obtain an accommodating cavity; injecting a specimen preservation solution into the accommodating cavity through an injection hole on the specimen display box; and sealing the liquid injection hole.
The specimen display box provided by the embodiment of the disclosure can realize the following technical effects:
adopt the sample show box that this disclosed embodiment provided to demonstrate the sample, improved the transparency to the sample show, solved the sample and sealed the opaque problem in epoxy.
The foregoing general description and the following description are exemplary and explanatory only and are not restrictive of the application.
Detailed Description
So that the manner in which the features and technical content of the embodiments of the present disclosure can be understood in detail, a detailed description of the embodiments of the present disclosure will be provided below. In the following description of the technology, for purposes of explanation, numerous details are set forth in order to provide a thorough understanding of the disclosed embodiments. However, one or more embodiments may be practiced without these details.
In some optional embodiments, the preparation method of the transparent specimen comprises sequentially subjecting the sample to be processed to the following processes: placing a sample to be processed in a first gradient alcohol solution, and performing dehydration treatment; placing the dehydrated sample in a first staining agent for first staining treatment; placing the sample subjected to the first dyeing treatment in a second gradient alcohol solution for rehydration; and placing the rehydrated sample in a trypsin solution, wherein solutes in the trypsin solution comprise trypsin and sodium borate.
The sample is placed in the trypsin solution, so that the success rate of the transparent specimen preparation is improved. For example, when the length of the sample to be processed is more than 7cm, the sample is placed in a trypsin solution, so that the problem of bone falling caused by muscle expansion of the sample can be prevented, and the success rate of preparing the transparent specimen is improved; meanwhile, the sample is placed in the trypsin solution, so that the problem of yellowing of muscle of the sample can be solved, and the attractiveness of the transparent specimen is improved. It is understood that the preparation method of the transparent specimen provided by the embodiment of the present disclosure may also be applied to a sample to be processed with a length less than or equal to 7 cm. It is understood that the length of the sample to be treated may be the length of the sample to be treated, similar to the height of a person.
It is understood that the "sample to be processed" may be a sample that has not been processed by the preparation method provided by the embodiment of the present disclosure, and the "sample" may be an intermediate sample that has been processed by a part of the steps in the preparation method provided by the embodiment of the present disclosure and needs to be processed next. Optionally, the method provided by the embodiment of the present disclosure is used for manufacturing a transparent specimen, and a fresh sample can be directly processed without performing dissection processing on the sample to be processed. "fresh" here may be a dead, non-dissected sample.
Placing a sample to be treated in a first gradient alcohol solution, and performing dehydration treatment;
in this step, the sample to be treated is placed in the first gradient alcohol solution, so that the sample to be treated can be dehydrated and the protein of the sample to be treated can be denatured.
Placing the dehydrated sample in a first staining agent for first staining treatment;
placing the sample subjected to the first dyeing treatment in a second gradient alcohol solution, and performing rehydration treatment, wherein the rehydration treatment can be understood as that the sample subjected to the dehydration treatment and having a lower water content absorbs water again;
in the step, the sample subjected to the first dyeing treatment is placed in the second gradient alcohol solution, so that the osmotic pressure of the sample can be adjusted, the sample is rehydrated, and the subsequent steps are facilitated.
And step four, putting the sample subjected to the rehydration into a trypsin solution, wherein solutes in the trypsin solution comprise trypsin and sodium borate.
In the step, the sample subjected to rehydration treatment is placed in a trypsin solution, so that the problem of sample skeleton falling caused by sample muscle expansion can be prevented, the integrity of the sample skeleton in the manufacturing process is improved, and the success rate of preparing the transparent specimen is further improved; meanwhile, the problem that muscle tissues of the sample to be treated are yellowed is prevented, and the attractiveness of the prepared transparent specimen is improved.
Optionally, the trypsin solution comprises the following components in parts by mass: trypsin, sodium borate and water 1-3:1.5-3: 80-120.
Sodium borate may also be referred to as borax, molecular formula Na2B4O7·10H2And O. In the trypsin solution, the mass ratio of trypsin to sodium borate to water can be 1:1.5:80, 1:1:40 or 1:2.4:100, so that the expansion of muscle tissues of the sample in the treatment process is reduced, the falling of bones of the sample is prevented, the integrity of the bones of the sample in the preparation process is improved, and the success rate of the transparent specimen is improved.
Optionally, the sample is placed in the trypsin solution for a time of 8h or more.
The time for which the sample is placed in the trypsin solution may be any one of 8h, 10h, 12h, 15h, 20h, 24h, 26h, 30h, 32h, 37h, 40h, 42h, 46h, 48h, 50h, and values in a range of values consisting of any two of these values. By adjusting the time for placing the sample in the trypsin solution, the muscle tissue of the sample can be prevented from expanding, the success rate of preparing the transparent specimen is improved, the time for placing the sample in the trypsin solution is not too long, otherwise, the muscle tissue of the sample can be scattered, and the preparation of the transparent specimen fails.
Optionally, when the length of the sample to be treated is less than 10cm, the time for placing the sample in the trypsin solution is less than or equal to 10 hours; when the length of the sample to be treated is more than or equal to 10cm and less than or equal to 20cm, placing the sample in the trypsin solution for 24-48 h; when the length of the sample to be treated is more than 20cm, the time for placing the sample in the trypsin solution is more than 48 h.
The time for which the sample is placed in the trypsin solution can be determined according to the length of the sample to be treated. For example, when the length of the sample to be treated is 7cm, the time for which it is placed in the trypsin solution is 7h, when the length of the sample to be treated is 8cm, the time for which it is placed in the trypsin solution is 8h, and when the length of the sample to be treated is 9cm, the time for which it is placed in the trypsin solution is 9 h; the time for placing the sample to be treated in the trypsin solution is 24 to 48 hours when the length of the sample to be treated is 10 to 20cm, for example, the time for placing the sample to be treated in the trypsin solution is 24 hours when the length of the sample to be treated is 10cm, the time for placing the sample to be treated in the trypsin solution is 40 hours when the length of the sample to be treated is 15cm, and the time for placing the sample to be treated in the trypsin solution is 48 hours when the length of the sample to be treated is 20 cm; when the length of the sample to be treated is more than 20cm, the time for placing the sample in the trypsin solution is more than 48 h. Not only can prevent the problem of skeleton shedding caused by the expansion of the muscle tissue of the sample, but also can not scatter the muscle tissue, thereby improving the success rate of preparing the transparent specimen.
Optionally, the first gradient alcohol solution comprises, in order by mass concentration: 70-80% alcohol solution, 90-98% alcohol solution; or, the second gradient alcohol solution comprises in sequence: 90-98% alcohol solution, 45-55% alcohol solution.
The mass concentration of the 70-80% alcohol solution in the first gradient alcohol solution can be any one of 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79% and 80%, and the range value of any two values, and the mass concentration of the 90-98% alcohol solution in the first gradient alcohol solution can be any one of 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97% and 98%, and the range value of any two values; the mass concentration of the 90-98% alcohol solution in the second gradient alcohol solution may be any one of 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, and 98%, and any two values of the range, and the mass concentration of the 45-55% alcohol solution in the second gradient alcohol solution may be any one of 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, and 55%, and any two values of the range.
Optionally, the method for performing dehydration on the sample to be processed by using the first gradient alcohol solution may be: the time for dehydration treatment can be reduced by placing the sample to be treated in 70-80% alcohol solution and 90-98% alcohol solution in sequence. At present, the sample to be processed is placed in 95% alcohol solution for dehydration treatment, the time of the dehydration treatment is long, which needs about 168 hours, the time of the dehydration treatment provided by the embodiment of the disclosure can be 70-74 hours, and when the temperature of the alcohol solution in the dehydration treatment process is 35 ℃, the time of the dehydration treatment can be 50 hours. For example, the dehydration treatment may be: the sample to be treated is placed in 75% alcohol solution for 24h, 95% alcohol solution for 24h, new 95% alcohol solution is replaced, and the sample is placed in new alcohol solution for 24 h.
Optionally, the method for rehydrating the sample with the second gradient alcohol solution may be: and (3) sequentially placing the sample in 90-98% alcohol solution, 45-55% alcohol solution and water, and adjusting the osmotic pressure of the sample to enable the sample to be rehydrated, thereby being more beneficial to the implementation of the subsequent steps. For example, the rehydration process may be: the sample was placed in 95% alcohol solution for 2h, 50% alcohol solution for 2h and water for 2h in this order. Optionally, the rehydration treatment may be performed under conditions of continuous shaking at a temperature of 35 ℃ to reduce the rehydration treatment time.
Optionally, the first stain is aliskiren.
The sample was stained with alisin blue for the first time. Alternatively, the first coloring agent may comprise aliskiren 1 mg: 50ml of acetic acid: 450g of water, i.e. the first colouring agent consisting of 1mg of aliskiren blue: 50ml of acetic acid: 450g of water is prepared, and the first coloring agent with the proportion is adopted, so that the concentration of acetic acid in the first coloring agent is reduced, the muscle tissue of the sample is prevented from falling off in the dyeing process, the success rate of preparing the transparent sample is improved, meanwhile, the muscle tissue of the sample is prevented from falling into the first coloring agent, the first coloring agent is not polluted, and the use frequency of the first coloring agent is improved.
Optionally, between the dehydration treatment and the first dyeing treatment, the method further comprises: and (4) placing the dehydrated sample in an acetic acid solution for peeling treatment.
The acetic acid solution is adopted to treat the sample after dehydration, the epidermis of the sample can be corroded, the epidermis of the sample can fall off in a short time, the step of manually peeling the sample is replaced, compared with the step of manually peeling, the peeling treatment provided by the embodiment of the disclosure is short in time, the tissues such as the muscle of the sample are not easily damaged, and the success rate of preparing the transparent specimen is improved.
Optionally, between the dehydration treatment and the peeling treatment, the method further comprises: and (4) placing the dehydrated sample in an enol solution for degreasing treatment. Wherein the enol solution can be replaced by gasoline, xylene, acetone, isoamyl acetate, etc.
When the fat content of the sample to be treated is high and the degreasing treatment is required, the degreasing treatment can be added between the dehydration treatment and the peeling treatment, and the degreasing treatment is understood as the treatment for removing the fat of the sample. Alternatively, when the temperature of the enol solution is 30 to 35 ℃, the time for which the sample is placed in the enol solution may be 24 hours.
Optionally, a dealkenizing treatment is also included. And (3) placing the degreased sample in an alcohol solution, and carrying out dealenol treatment to remove enol in the sample. Alternatively, when the temperature of the alcohol solution is 30-35 ℃, the dealcoholization time may be 30 min.
Optionally, after the sample is treated with the trypsin solution, the method further comprises:
placing the sample treated by the trypsin solution in a KOH solution with the mass concentration of 2%, and carrying out transparent treatment on the muscle tissue of the sample for 4 hours;
placing the transparent sample in a second staining reagent, and staining the bone of the sample, wherein the second staining reagent may optionally comprise 0.5% KOH and a saturated alizarin red alcohol solution, and the staining time may optionally be 48h when the temperature of the second staining reagent is 25 ℃;
placing the sample subjected to the second dyeing treatment in a hydrogen peroxide solution for decoloring, wherein the sample subjected to the second dyeing treatment is dark and dark purple, and can be decolored by using the hydrogen peroxide solution, optionally, the mass concentration of the hydrogen peroxide solution can be 1%, optionally, if the decoloring treatment time is long, a pink bone specimen can be obtained, and if the decoloring treatment time is short, a dark purple bone specimen can be obtained;
placing the decolored sample in a gradient glycerol solution to obtain a transparent sample, wherein optionally, the gradient glycerol solution comprises 20% glycerol solution, 50% glycerol solution and 100% glycerol solution by mass concentration, and placing the decolored sample in the 20% glycerol solution, the 50% glycerol solution and the 100% glycerol solution in sequence to obtain the transparent sample.
According to the preparation method of the transparent specimen, provided by the embodiment of the disclosure, the success rate of the preparation of the transparent specimen is improved, the muscle tissue of the prepared transparent specimen does not generate a yellowing phenomenon, and the attractiveness of the transparent specimen is improved. Simultaneously, the transparent specimen that this disclosed embodiment provided is non-deformable, has improved the save time of transparent specimen.
Optionally, the sample to be processed comprises: teleost, shellfish, shrimp, crab or insect. For example, the sample to be treated may be cartilaginous fishes such as shark, teleostearic fishes such as sturgeon, teleostearic fishes such as whitebait, and shellfish such as cephalopod such as cuttlefish, bivalve shellfish such as mactra philippinarum, and the insect may be dragonfly larva. The shrimp can be Mysidacea, Penaeus orientalis, Penaeus vannamei, Penaeus monodon, etc. The crab can be eriocheir sinensis, flat back, etc. In addition, the preparation method of the transparent specimen provided by the embodiment of the disclosure can also be applied to mammals and reptiles, wherein the mammals can be American minks, mice, rats, hamsters and the like, and the reptiles can be turtles, lizards, snakes and the like.
The present disclosure also provides a transparent specimen.
In some alternative embodiments, the transparent specimen is obtained by the preparation method described previously.
The transparent specimen provided by the embodiment of the disclosure is very beautiful and can be used for teaching of deplaning and scientific popularization and the like.
The present disclosure simultaneously provides a specimen display box, include: a first receiving member; the second accommodating part and the first accommodating part form an accommodating cavity; the specimen fixing piece is arranged on the first accommodating piece or the second accommodating piece; and the liquid injection hole is arranged in at least one of the first accommodating part and the second accommodating part.
The first accommodating part and the second accommodating part form an accommodating cavity, and it can be understood that when the first accommodating part and the second accommodating part need to form the accommodating cavity, the accommodating cavity can be obtained by buckling the first accommodating part and the second accommodating part and the like, and when the first accommodating part and the second accommodating part do not need to form the accommodating cavity, the first accommodating part and the second accommodating part are of split structures. The size of the first receiving member and the second receiving member is not limited too much, and may be determined according to the size of a specimen to be displayed, for example. The first receiving member is fastened to the second receiving member to form a receiving cavity, and it is understood that the first receiving member serves as a bottom and the second receiving member serves as a cover, and the second receiving member is fastened to the first receiving member to form a receiving cavity, such as a bottom and a cover of a culture dish.
The sample mounting is used for fixing the sample of treating fixed, annotates the liquid hole and is used for holding the intracavity and pour into sample retaining fluid into.
Alternatively, the specimen herein may be a transparent specimen prepared as described above. According to the preparation method of the transparent specimen, the damage to the skeleton of the sample is small, the hard and hardly damaged transparent specimen is obtained, when the specimen fixing piece of the specimen display box provided by the embodiment of the disclosure is used for fixing, the skeleton of the transparent specimen is not easy to fall off, and the transparent specimen is easily and completely displayed. Adopt this specimen exhibition box that this disclosed embodiment provided to demonstrate the specimen, improved the transparency to the specimen show.
Optionally, at least one of the first receiving member or the second receiving member is a transparent member.
The transparent first and/or second receiving members enhance the light transmission of the specimen to be displayed. Optionally, the first accommodating part and the second accommodating part are transparent parts, and the sample to be displayed can be comprehensively displayed.
Optionally, the first receptacle comprises: a first bottom portion; the first side wall, constitute first recess form with first bottom, and the second receiver includes: a second bottom; the second side wall and the second side wall form a second groove shape, and the diameter of the first bottom is smaller than that of the second bottom.
Optionally, the first sidewall is perpendicular to the first bottom, and the second sidewall is perpendicular to the second bottom. The diameter of the first bottom is smaller than that of the second bottom, and the second accommodating part can be buckled on the first accommodating part to form an accommodating cavity. Optionally, the difference between the diameters of the second bottom and the first bottom is 0.01-0.1cm, which is beneficial to improving the sealing performance of the obtained accommodating cavity. The shape of the first bottom and the second bottom can be round, polygonal or other irregular shapes, so as to improve the aesthetic property of the specimen display box. When the first bottom and the second bottom are circular, the first accommodating part is similar to the bottom of a culture dish, the second accommodating part is similar to the cover of the culture dish, and the cover of the culture dish is covered on the bottom of the culture dish to form the accommodating cavity.
Optionally, the specimen holder is fixedly disposed on the first bottom portion or the second bottom portion.
The specimen fixing piece can be fixed on the first bottom or the second bottom through fixed connection modes such as gluing and threaded connection. Optionally, the specimen fixing member is integrally formed with the first accommodating member or the second accommodating member, so that the connection stability of the specimen fixing member with the first accommodating member or the second accommodating member is improved.
Optionally, the specimen holder is one or more fixation posts.
When the sample mounting is more than one fixed column, more than one fixed column can all set up in the first bottom of first holding, also can all set up in the second bottom that the second held. Optionally, the fixing column is perpendicular to the first bottom or the second bottom, or the fixing column forms an acute angle or an obtuse angle with the first bottom or the second bottom. Optionally, more than one specimen mount is disposed on the first base portion having the smaller diameter. When the sample mounting is more than one fixed column, a plurality of fixed columns can be arranged for equidistance or not, can set up the relative position of a plurality of fixed columns according to the size and the skeleton distribution characteristics of the sample of waiting to demonstrate.
Optionally, the fixed posts are 1-10mm in diameter.
The diameter of the fixing column should not be too large, otherwise the bone of the sample to be displayed is damaged. For example, the diameter of the fixation post may be defined according to the size of the gap between two adjacent bones of the specimen to be displayed. Optionally, the fixing column includes a first end fixed to the first accommodating part or the second accommodating part and a second end opposite to the first end, a vertical distance from the first end to the second end is defined as a height of the fixing column, and the height of the fixing column is smaller than a width of the accommodating cavity, so as to improve the sealing performance of the accommodating cavity.
Optionally, the pour hole is provided in at least one of the first side wall or the second side wall.
Optionally, the first side wall is provided with a first liquid injection hole, and the second side wall is provided with a second liquid injection hole matched with the first liquid injection hole. The matching of the first pour hole and the second pour hole herein can be understood as follows: when first holding is detained mutually with the second holding and is obtained holding the chamber, first lateral wall overlaps with the second lateral wall, and the hole also overlaps are annotated with the second to first notes liquid hole, and the accessible is first annotate liquid hole and second and annotate liquid hole and to holding intracavity injection specimen preservation liquid. Alternatively, a syringe may be used to inject the specimen preservation solution into the first injection hole. Optionally, the inner diameter of the liquid injection hole is 0.3-1 cm.
The embodiment of the disclosure also provides a specimen display method, which includes:
s101: fixing a specimen to be displayed on a specimen fixing piece of the specimen display box;
the specimen display kit herein may be the specimen display kit described above. Alternatively, the specimen may be secured by passing the space between the two connected bones of the specimen through a specimen mount.
S102: connecting a first accommodating piece and a second accommodating piece of the specimen display box to obtain an accommodating cavity;
after the first containing part is connected with the second containing part, the containing cavity can be communicated with the outside through the liquid injection hole. When the first accommodating part is in a first groove shape formed by the first bottom and the first side wall and the second accommodating part is in a second groove shape formed by the second bottom and the second side wall, the first accommodating part and the second accommodating part can be buckled with each other, and a gap between the first side wall and the second side wall is glued to obtain the accommodating cavity.
S103: injecting a specimen preservation solution into the accommodating cavity through an injection hole on the specimen display box;
alternatively, an injector can be used to inject the specimen preservation solution into the containing cavity through the injection hole, and the specimen preservation solution can be glycerol.
S104: and sealing the liquid injection hole.
After the containing cavity is filled with the specimen preserving fluid, the fluid injection hole is sealed, and the specimen displaying step is completed.
The specimen display method provided by the embodiment of the disclosure improves the transparency of specimen display, and solves the problems that the transparent specimen is fixedly sealed in the epoxy resin and the epoxy resin is not transparent and the epoxy resin is atomized; meanwhile, the problem that the transparent specimens of flat and side flat organisms are difficult to display is solved.
Optionally, the first receiving member and the second receiving member are connected by a first sealant.
The first sealant can be ultraviolet light curing adhesive or AB adhesive.
Optionally, the liquid injection hole is sealed by a second sealant.
The first sealant can be ultraviolet light curing glue or hot melt glue, and can enable the liquid injection hole to be sealed quickly.
The ranges of the mass fraction of each component described in the present application include not only two end values of each range, but also all values between the two end values and a range value consisting of any two values between the two end values. For example, a 70% -80% alcohol solution, where 70% -80% includes not only the two endpoints 70%, 80%, but also all values between 70% -80%, and any two values between 70% -80% (e.g., 72%, 77%) inclusive of the range value (e.g., 72% -77%).
Claims (10)
1. A specimen display box, comprising:
a first receiving member;
the second accommodating piece and the first accommodating piece form an accommodating cavity;
a specimen holder provided in the first receiving member or the second receiving member;
and the liquid injection hole is arranged in at least one of the first accommodating part and the second accommodating part.
2. The specimen display cassette according to claim 1,
at least one of the first accommodating member or the second accommodating member is a transparent member.
3. The specimen display cassette according to claim 1,
the first receiving member includes:
a first bottom portion;
a first side wall forming a first groove shape with the first bottom,
the second receiving member includes:
a second bottom;
a second side wall forming a second groove shape with the second side wall,
the diameter of the first bottom is smaller than the diameter of the second bottom.
4. The specimen display cassette according to claim 3,
the specimen fixing piece is fixedly arranged at the first bottom or the second bottom.
5. The specimen display cassette according to claim 1 or 4,
the specimen fixing piece is one or more than one fixing column.
6. The specimen display cassette according to claim 5,
the diameter of the fixed column is 1-10 mm.
7. The specimen display cassette according to claim 3,
the liquid injection hole is arranged on at least one of the first side wall or the second side wall.
8. A specimen presentation method, comprising:
fixing a specimen to be displayed on a specimen fixing piece of the specimen display box;
connecting a first accommodating piece and a second accommodating piece of the specimen display box to obtain an accommodating cavity;
injecting a specimen preservation solution into the accommodating cavity through an injection hole on the specimen display box;
and sealing the liquid injection hole.
9. The specimen presentation method according to claim 8,
and connecting the first accommodating part and the second accommodating part by adopting first sealant.
10. The specimen presentation method according to claim 8,
and sealing the liquid injection hole by adopting a second sealant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911166260.5A CN112825846A (en) | 2019-11-25 | 2019-11-25 | Specimen display box and specimen display method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911166260.5A CN112825846A (en) | 2019-11-25 | 2019-11-25 | Specimen display box and specimen display method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112825846A true CN112825846A (en) | 2021-05-25 |
Family
ID=75922970
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911166260.5A Pending CN112825846A (en) | 2019-11-25 | 2019-11-25 | Specimen display box and specimen display method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112825846A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1480038A (en) * | 2003-07-28 | 2004-03-10 | 王孔宝 | Embedding material biological sample and method for making specimen of human body |
| CN203562118U (en) * | 2013-11-25 | 2014-04-23 | 乃村工艺建筑装饰（北京）有限公司 | Specimen box |
| CN206804381U (en) * | 2017-06-06 | 2017-12-26 | 广州艺灵教学用标本有限公司 | A kind of resin sample forming and hardening equipment |
| CN108839892A (en) * | 2018-08-10 | 2018-11-20 | 中国科学院古脊椎动物与古人类研究所 | A kind of sample method for preserving and multi-functional sample case assembly and Riker mount |
| CN110278939A (en) * | 2019-07-03 | 2019-09-27 | 秦家哲 | A kind of production method and its application of transparent stained preparation |
| TWM584263U (en) * | 2019-07-04 | 2019-10-01 | 鄭艾蒙 | Cultural and creative objects including transparent biological specimens |
-
2019
- 2019-11-25 CN CN201911166260.5A patent/CN112825846A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1480038A (en) * | 2003-07-28 | 2004-03-10 | 王孔宝 | Embedding material biological sample and method for making specimen of human body |
| CN203562118U (en) * | 2013-11-25 | 2014-04-23 | 乃村工艺建筑装饰（北京）有限公司 | Specimen box |
| CN206804381U (en) * | 2017-06-06 | 2017-12-26 | 广州艺灵教学用标本有限公司 | A kind of resin sample forming and hardening equipment |
| CN108839892A (en) * | 2018-08-10 | 2018-11-20 | 中国科学院古脊椎动物与古人类研究所 | A kind of sample method for preserving and multi-functional sample case assembly and Riker mount |
| CN110278939A (en) * | 2019-07-03 | 2019-09-27 | 秦家哲 | A kind of production method and its application of transparent stained preparation |
| TWM584263U (en) * | 2019-07-04 | 2019-10-01 | 鄭艾蒙 | Cultural and creative objects including transparent biological specimens |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Takayanagi et al. | An ovary‐stimulating factor in the shrimp, Paratya compressa | |
| Yao et al. | Biochemical compositions and digestive enzyme activities during the embryonic development of prawn, Macrobrachium rosenbergii | |
| CN106667982A (en) | Method for preparing zebrafish thrombus model | |
| CN112825846A (en) | Specimen display box and specimen display method | |
| CN112825847A (en) | Transparent specimen and preparation method thereof | |
| Hardege et al. | Spawning synchrony in Arenicola marina: evidence for sex pheromonal control | |
| Bruce et al. | Lipid class and fatty acid composition of eggs of Atlantic halibut, Hippoglossus hippoglossus (L.), in relation to egg quality in captive broodstock | |
| CN104977194A (en) | Method of accelerating sample treatment with addition of graphene | |
| CN112825845A (en) | Specimen display method | |
| Watson et al. | Rapid isolation of ecdysteroids from crustacean tissues and culture media using Sep-Pak C18 cartridges | |
| CN103524772A (en) | Anti-liver cell aging co-fixation electric biomaterial and application thereof | |
| Hasumi | Seasonal fluctuations of female reproductive organs in the salamander Hynobius nigrescens | |
| CN113812393A (en) | Method for manufacturing mouse double-dyeing transparent skeleton plastic package embedding specimen | |
| Rozenfeld et al. | Hydroxyurea: an inhibitor of the differentiation of choanocytes in fresh‐water sponges and a possible agent for the isolation of embryonic cells | |
| Kalthoff et al. | Mitochondria and polarity in the egg of Smittia spec.(Diptera, Chironomidae): UV irradiation, respiration measurements, ATP determination and application of inhibitors | |
| CN103409370A (en) | Culture medium for lymphocyte culture and application thereof | |
| CN103076283A (en) | Method for observing developmental process of salmonidae roes | |
| Wodzicki et al. | Maturing pine tracheids: Organization of intravacuolar cytoplasm | |
| Strecker et al. | Permeabilization, staining and culture of living Drosophila embryos | |
| CN205322337U (en) | Draw operating means of two shell shellfish hemolymphs | |
| Kuhn et al. | A modified polyester embedding medium for sectioning | |
| CN112393957A (en) | Simple preparation method of fish chromosome karyotype | |
| CN1428419A (en) | Method for preparing fertilized ovum chromosome | |
| Gagnon | Fungus culture on cellophane membrane for cytochemical tests | |
| CN101612051B (en) | Method for checking crab ovary development by living body puncture technology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210525 |