CN205322337U - Draw operating means of two shell shellfish hemolymphs - Google Patents

Draw operating means of two shell shellfish hemolymphs Download PDF

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Publication number
CN205322337U
CN205322337U CN201620009773.0U CN201620009773U CN205322337U CN 205322337 U CN205322337 U CN 205322337U CN 201620009773 U CN201620009773 U CN 201620009773U CN 205322337 U CN205322337 U CN 205322337U
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CN
China
Prior art keywords
capsule
hemolymph
shellfish
cuboid metal
operation device
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201620009773.0U
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Chinese (zh)
Inventor
李家乐
彭茂潇
牛东红
杜蕴超
卫侃韵
王飞
王泽�
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Shanghai Maritime University
Shanghai Ocean University
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Shanghai Maritime University
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Publication date
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Priority to CN201620009773.0U priority Critical patent/CN205322337U/en
Application granted granted Critical
Publication of CN205322337U publication Critical patent/CN205322337U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model relates to a draw operating means of two shell shellfish hemolymphs, this operating means comprises 2 detachable construction and fixed knot structure, fixed knot construct including a cuboid metal frame, detachable construction include a plastic plates and plastics system drawer form capsule, plastic plates arrange the top surface of cuboid metal frame in, be carved with a recess among them, recess bottom be equipped with cylindric aperture, plastics system drawer form capsule arrange in on the inside bottom surface of cuboid metal frame. Its advantage shows: the utility model discloses the device can promote two shell shellfish hemolymph total amounts and the purity of extracting to the device simple structure, operation process is simple effective during the use.

Description

A kind of operation device extracting bivalve shellfish hemolymph
Technical field
This utility model relates to bivalve shellfish hemolymph extraction element technical field, specifically, is a kind of operation device extracting bivalve shellfish hemolymph。
Background technology
Nowadays being the epoch of ocean, culture fishery develops rapidly。Bivalve shellfish is as important economy aquatic animals, and in production practices, disease is always up the key factor of high yield, and studied person is paid close attention to always。And hemolymph is as the important immuning tissue of shellfish, there is high researching value。
Due to bivalve generic open pipe formula blood circulation, the collection of its hemolymph is always up difficult point。At present, researcher multi injector and syringe needle, but traditional to take the method syringe needle of blood with syringe and syringe needle meticulous, easily blocks during absorption, the amount often leading to draw is inadequate, and is easily extracted into body fluid and impure。
Based on above-mentioned reason, in the extraction process of hemolymph, the problem that purity is not high, amount is always up puzzlement researcher less。Accordingly it is desirable to the method finding a large amount of and pure shellfish hemolymph of a kind of simple and effective extraction。
Chinese patent application: CN2712307Y, application number is 200420016495.9, date of application is on July 26th, 2004, one utility model patent discloses the device of a kind of extracting pulmonary alveolus irrigating solution from animal lungs, the bottom of this device is a support platform, foam plastics in order to fixing animal is placed above, top is an injector operations platform, support with four pillars and fix between two platforms, there is an aperture platform center, plug in the hard plastic tube of a hollow, the equal sleeving plastic flexible pipe of upper and lower side of hard plastic tube, the upper end of upper end plastic flexible pipe connects syringe, the lower end connecting insertion of lower end plastic flexible pipe, intubate for being connected with animal trachea。The design of this utility model device is simple, it is adaptable to the extraction of bronchoalveolar lavage fluid in animal lung, but the extraction for bivalve shellfish hemolymph does not provide coherent reference in fact。So the device about the shellfish hemolymph extracting lot of pure yet there are no report。
In sum, bivalve shellfish economy aquatic animals research field is needed badly a kind of easy and simple to handle, and effective use can extract the operation device of enough pure hemolymphes simultaneously。
Summary of the invention
The purpose of this utility model is for the extracted amount and the DNA purity that improve shellfish hemolymph, it is provided that a kind of operation device extracting bivalve shellfish hemolymph。
For achieving the above object, this utility model adopts the technical scheme that: a kind of operation device extracting bivalve shellfish hemolymph, this operation device is made up of 2 demountable structures and fixed structure, described fixed structure includes a cuboid metal framework, described demountable structure includes one piece of plastic board and a plastics drawer-like capsule, described plastic board is placed in the end face of cuboid metal framework, a groove it is carved with in the middle of it, described bottom portion of groove is provided with cylindric aperture, described plastics drawer-like capsule is placed on cuboid metal framework inner bottom surface。
The described bottom surface of cuboid metal framework, the back side and two sides are wood based panel front cover。
The described little box top of plastics drawer-like is not closed, for receiving and remove the liquid flowed down from groove。
The outer surface in described plastics drawer-like capsule front is provided with a handle, it is simple to take out by capsule。
This utility model advantage is in that:
1, this utility model device can promote the bivalve shellfish hemolymph total amount and purity thereof extracted。
2, this utility model apparatus structure is simple, and during use, operating process is simply effective。
Accompanying drawing explanation
Accompanying drawing 1 is the structural representation of this utility model operation device。
Accompanying drawing 2 is the plastic board structural representation of this utility model operation device。
Accompanying drawing 3 is pipettor gun head cut place schematic diagram。
Detailed description of the invention
Also with reference to accompanying drawing, this utility model is further described below in conjunction with embodiment。
The accompanying drawing labelling related in accompanying drawing and ingredient are as follows:
1. metal framework 2. plastic board 3. drawer-like capsule 4. handle
5. groove 6. aperture 7. rifle head cut place。
As shown in Figure 1, a kind of operation device extracting bivalve shellfish hemolymph of this utility model, this operation device is made up of 2 demountable structures and fixed structure。Fixed structure includes a cuboid metal framework 1, and demountable structure includes one piece of plastic board 2 being placed in cuboid metal framework 1 end face and a plastics drawer-like capsule 3。Described plastics drawer-like capsule 3 is placed on cuboid metal framework 1 inner bottom surface。The front of drawer-like capsule 3 is provided with a little handle 4, it is simple to extract drawer-like capsule 3, removes liquid。The end face of drawer-like capsule 3 does not have front cover to be opening shape, in order to receive the liquid flowed down in groove 5。General, the length of drawer-like capsule 3 respectively 18cm, 10cm, 6cm so that it is can just be positioned in metal framework 1。
As shown in Figure 2: be carved with a groove 5 in the middle of described plastic board 2, bottom groove 5, be provided with cylindric aperture 6。General, the length of plastic board 2 is 20cm, and wide for 12cm, thickness is about about 3cm, and for bearing the body weight of bivalve shellfish, its thickness can increase and decrease as required。The groove 5 of plastic board 2 mid portion is to be about as 6cm, width is about 1.3cm, height is about 1.2cm, groove 5 is for placing blood bivalve shellfish to be taken, so the value of its length, width and height can be adjusted according to practical operation, it should be noted that the numerical value in this explanation is for general bivalve shellfish。The numerical value of groove 5 length, width and height is chosen and should be met: the value of length should be greater than 2 times that blood bivalve shellfish shell to be taken is wide, the shell that the value of width is about blood bivalve shellfish to be taken is high, the value of height can be stood and not affect at plastic board with it and histioid operation of bivalve is advisable, but can not more than the thickness of plastic board 2, in order to avoid the body weight of bivalve shellfish cannot be born。The cylindric aperture 6 of groove 5 base plate spills body fluid in the drawer-like capsule 3 below device for arranging, and aperture 6 diameter is 2~3mm, and generally arranging little hole number is 20~40。
The specific implementation process of this utility model device is as follows:
First blood bivalve shellfish medical cotton ball to be taken is blotted sea surface, with alcohol swab wiping surface。By shellfish front (the morphologic front of shellfish) down, in the groove 5 standing on this device is vertically placed, towards observer, by back of the body side (the morphologic back of shellfish), from back of the body side, hinge is forwards drawn disconnected with No. 23 dissecting knife, and front portion closed shell flesh is drawn disconnected。Draw and have no progeny to go out having substantial amounts of body of shellfish liquid stream, after body fluid drains off, shellfish it is inverted in this device groove 5 and slightly struts (can not overexert, the boundary differentiated between outer embrane and shell can be known) with hands or pliers (big individual bivalve shellfish uses)。Then prolong the edge between outer embrane and shell gently down (i.e. the morphologic rear of shellfish) with dissecting knife and scratch (near pricardial coelom, never scratching pricardial coelom), until cutting off rear closed shell flesh。Then side shell is slowly opened, make that bivalve is open and flat to be stood within groove 5, scratching body cavity with dissecting knife makes the coelomic fluid of internal residual flow out (body fluid flows in the plastic drawer shape capsule 3 of lower section along the aperture 6 of groove 5) and with cotton balls, the body fluid of Sinonovacula constricta tissue surface be blotted, and pads appropriate cotton balls with aspirates tissue transudate below abdominal foot and water pipe。Being taken off by moveable plastic board 2 lies against in laboratory table, by open and flat for Sinonovacula constricta thereon, with tweezers (9cm fine dissection tip tweezers) prolong auricular edge needle the outer embrane above pricardial coelom and pricardial coelom film and it is dialled gently from, make heart come out。With 1000ul pipettor gun head (as shown in Figure 3, remove about 3-4mm tip), stimulate abdominal foot to make it shrink the purpose reaching congestive heart, when treating that heart is heaved, draw rapidly with pipettor and make film quality rupture of heart and draw hemolymph。After having operated, if the long-pending handle 4 that completely can pass through of plastic drawer shape capsule 3 liquid take out drawer-like capsule 3 and clears up body fluid。
The above is only preferred implementation of the present utility model; it should be pointed out that, for those skilled in the art, under the premise without departing from this utility model principle; can also making some improvement and supplement, these improve and supplement and also should be regarded as protection domain of the present utility model。

Claims (4)

1. the operation device extracting bivalve shellfish hemolymph, it is characterized in that, described operation device is made up of 2 demountable structures and fixed structure, described fixed structure includes a cuboid metal framework, described demountable structure includes one piece of plastic board and a plastics drawer-like capsule, described plastic board is placed in the end face of cuboid metal framework, a groove it is carved with in the middle of it, described bottom portion of groove is provided with cylindric aperture, and described plastics drawer-like capsule is placed on cuboid metal framework inner bottom surface。
2. a kind of operation device extracting bivalve shellfish hemolymph according to claim 1, it is characterised in that the described bottom surface of cuboid metal framework, the back side and two sides are wood based panel front cover。
3. a kind of operation device extracting bivalve shellfish hemolymph according to claim 1, it is characterised in that the described little box top of plastics drawer-like is not closed, for receiving and remove the liquid flowed down from groove。
4. a kind of operation device extracting bivalve shellfish hemolymph according to claim 1, it is characterised in that the outer surface in described plastics drawer-like capsule front is provided with a handle, it is simple to take out by capsule。
CN201620009773.0U 2016-01-06 2016-01-06 Draw operating means of two shell shellfish hemolymphs Expired - Fee Related CN205322337U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201620009773.0U CN205322337U (en) 2016-01-06 2016-01-06 Draw operating means of two shell shellfish hemolymphs

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201620009773.0U CN205322337U (en) 2016-01-06 2016-01-06 Draw operating means of two shell shellfish hemolymphs

Publications (1)

Publication Number Publication Date
CN205322337U true CN205322337U (en) 2016-06-22

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CN201620009773.0U Expired - Fee Related CN205322337U (en) 2016-01-06 2016-01-06 Draw operating means of two shell shellfish hemolymphs

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111481315A (en) * 2020-02-13 2020-08-04 中国水产科学研究院黄海水产研究所 Shellfish dissecting device

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111481315A (en) * 2020-02-13 2020-08-04 中国水产科学研究院黄海水产研究所 Shellfish dissecting device

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C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20160622

Termination date: 20180106

CF01 Termination of patent right due to non-payment of annual fee