CN103165009B - The method for making of the transparent embedding cross-section specimen of a kind of human acupoint - Google Patents

The method for making of the transparent embedding cross-section specimen of a kind of human acupoint Download PDF

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Publication number
CN103165009B
CN103165009B CN201310070913.6A CN201310070913A CN103165009B CN 103165009 B CN103165009 B CN 103165009B CN 201310070913 A CN201310070913 A CN 201310070913A CN 103165009 B CN103165009 B CN 103165009B
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acupuncture
human
nameplate
making
section specimen
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CN103165009A (en
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程明亮
赵顺
王爱芳
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HENAN ZHONGBO BIO-PLASTINATION TECHNIQUE Co Ltd
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HENAN ZHONGBO BIO-PLASTINATION TECHNIQUE Co Ltd
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Abstract

The present invention relates to the method for making of the transparent embedding cross-section specimen of a kind of human acupoint, its selection meets normal human's sample that Asian plants physiometry standard, after lavation, conventional preservative treatment, according to normal human's anatomy body surface symbol and Shu Points orientation related assays data, body surface location carried out to the acupuncture points on the human body that will show and penetrates colored plastic pin as mark at the particular location at corresponding acupuncture point, then carrying out freezing, sawing, bleaching, dehydration, infiltration, contact pin lay nameplate, die-filling, embedding and solidification.Adopt the acupuncture-moxibustion cross-section specimen that the method makes, tissue transparency is high; Inserting needle direction, depth of needle, acupuncture points on the human body dark face structure to be adjoined etc. very clear, nonpoisonous and tasteless, preserves to safeguard simple, farthest meets the needs of acupuncture teaching and the study of social crowd's popular science knowledge.

Description

The method for making of the transparent embedding cross-section specimen of a kind of human acupoint
Technical field
The invention belongs to preparation of specimen's technical field, be specifically related to making and the identification method of the transparent embedding cross-section specimen of a kind of human acupoint.
Background technology
For a long time, anatomy personnel to traditional Chinese medicine be correlated with sample research less, particularly the related entities sample of science of acupuncture and moxibustion is rare especially.Everybody understands less to the mechanism of action of acupuncture-moxibustion, and just set forth from channels and collaterals aspect, this just needs a kind of sample intuitively can showing acupuncture point relevant knowledge and deep structure.
2004, Zhengzhou was defended religion experimental article development center and has been produced first set acupuncture-moxibustion acupuncture point section sample in kind.But its store method is still make antiseptic with formaldehyde, encapsulates with plexiglass box.There is following problem in this acupuncture-moxibustion sample: 1) formaldehyde is larger to human injury; 2) the liquid storage time is long rear variable mixed, and impact uses; 3) Acupuncture needle easy-to-rust; 4) carry inconvenience, can only use as display; 5) serviceable life is shorter.
2011, religion experimental article development center is defended again on the basis of plastifying technology in Zhengzhou, made human meridian point's entirety plasticizing sample, this type of sample can demonstrate body surface location and the traveling of collaterals of human acupuncture points on the human body clearly, but it lacks displaying in deep structure neighbouring relationship.
2012, Shanghai Univ. of Traditional Chinese Medicine's development acupuncture-moxibustion section embedding, but be all based on local, and be only applied to less section, and complete channels and collaterals traveling can not be shown on same section.
Summary of the invention
The object of the invention is the making and the identification method that provide the transparent embedding cross-section specimen of a kind of human acupoint, in order to meet the demand of acupuncture teaching and Popular Science Education.
For achieving the above object, the present invention adopts following technical scheme:
The method for making of the transparent embedding cross-section specimen of a kind of human acupoint, its selection meets normal human's sample that Asian plants physiometry standard, after lavation, conventional preservative treatment, according to normal human's anatomy body surface symbol and Shu Points orientation related assays data, body surface location carried out to the acupuncture points on the human body that will show and penetrates colored plastic pin as mark at the particular location at corresponding acupuncture point, then carrying out freezing, sawing, bleaching, dehydration, infiltration, contact pin lay nameplate, die-filling, embedding and solidification.
Concrete, according to anatomy and science of acupuncture and moxibustion teaching needs during sawing, sawing becomes different orientation and different thickness.
The H of mass concentration 10-15% is selected during bleaching 2o 2, slowly carry out, to guarantee dark face bleaching effect.
During infiltration, pressure aspirates from low to high, pressure limit-0.04 ~-0.08MPa, stops when tissue becomes translucent.
The pin that described contact pin laying is selected in nameplate process and nameplate are medical stainless steel material.
Nameplate can be embedded in resin plane according to demand, also can be free on around section.
Autopsy material should be fresh as far as possible, before anticorrosion, first carry out lavation, gets rid of extravasated blood.
After solidification terminates, through slightly repairing, displaying can be installed.Install and show that employing stainless steel runing rest is shown, the thick tempered glass clamping of double-deck 8mm, upper lower bayonet slot is fixed.
The innovative point of the inventive method is: on the basis of original epoxy resin embedding, changes its multiple link, the section transparency itself is increased, then is indicated by imbedding needle, shows around acupuncture points on the human body clearly and the dependency structure in dark face.Simultaneously because its transparency is higher, can clearly demonstrate inserting needle direction, depth of needle, pathway structure etc. again, particularly some dangerous acupuncture points, just can understand the reason wanting controlling depth, direction etc. in acupuncture treatment process after allowing people see sample.
The maximum advantage of the inventive method makes tissue become translucent, then coordinate acupuncture treatment to indicate, clear display acupuncture points on the human body dark face structure and adjoining and the inserting needle direction degree of depth etc.Achieve the perfect adaptation of normal human's anatomy and acupuncture teaching, exhibition method is practical flexibly, existing good display effect, for convenience detach again, greatly improves the utilization factor of sample.In addition, also can be used as popular science propaganda teaching material, allow social crowd understand the secret of Geng Duo motherland medical science.
With the acupuncture-moxibustion cross-section specimen that the present invention makes, both can do locally thinner, also can do the overall thicker section of whole body, local thickness 2mm, neck trunk lower limb sagittal, crown 7 mm.Nonpoisonous and tasteless, preserve and safeguard simply, farthest meet the needs of acupuncture teaching and the study of social crowd's popular science knowledge.
The present invention is based on normal human's anatomy, traditional Chinese medical science science of acupuncture and moxibustion related content is intuitively showed a kind of technical method of student and correlated crowd.With the cross-section specimen that the method makes, eliminate the harm of formaldehyde to human body, the holding time is longer, not fragile, easy to use.
Accompanying drawing explanation
Fig. 1 is the whole structure figure of the sample adopting the inventive method to make;
Fig. 2 is the partial result figure of the sample adopting the inventive method to make, and is specially trunk median sagittal tangent plane, display big vertebra, Taotao, Shenzhu.
Embodiment
Below by way of specific embodiment, technical scheme of the present invention is described, but protection scope of the present invention is not limited thereto.
embodiment 1
The making of the transparent embedding cross-section specimen of a kind of human acupoint and identification method, its selection meets normal human's sample that Asian plants physiometry standard, after lavation, conventional preservative treatment, according to normal human's anatomy body surface symbol and Shu Points orientation related assays data, body surface location carried out to the acupuncture points on the human body that will show and penetrates colored plastic pin as mark at the particular location at corresponding acupuncture point, then carrying out freezing, sawing, bleaching, dehydration, infiltration, contact pin lay nameplate, die-filling, embedding, solidification, finishing and install and show.Specific implementation method is as follows:
1, lavation:
Select the death time shorter, and the normal person's corpse meeting Asia body measurement standard is as sample.Before anticorrosion, first pour into from artery with physiological saline, by vena cava opening, allow extravasated blood be expelled to efflux and become clear.Slightly long to the death time, thrombolytics should be added in physiological saline.The object of process like this allows extravasated blood in body discharge completely in time, in order to avoid affect transparent effect.
2, anticorrosion fixing:
After lavation completes, do preservative treatment with 10% formalin.The anticorrosion corpse fixed should soak and can use for 60 days in formalin.
3, Shu Points orientation:
Taken out by material, surface clean is clean, according to normal human's anatomy body surface symbol and Shu Points orientation related assays result, positions the acupuncture points on the human body that need show, and penetrates colored plastic pin as mark at the particular location at corresponding acupuncture point.Finally according to the orientation of wanted sawing, will connect in the crayon setting-out of conplane acupuncture points on the human body.If what do is follow through section, corresponding channels and collaterals should be coupled together, as foundation during sawing.
4, freezing:
Shu Points orientation completes, and is fixed on horizontal shelf according to normal human's anatomy posture by sample, freezing at-20 degree temperature.
5, sawing:
Should carry out in strict accordance with mark setting-out during sawing, by THICKNESS CONTROL at zone of reasonableness, too thin easy damage, too thick affects transparent effect.Therefore, head level, sagittal, crown thickness is 3mm; Trunk horizontal section thickness is 5mm; Neck trunk lower limb sagittal plane, coronal-plane thickness are 7mm.After sawing has been repaired, the reply kerf on section top layer and the burr of surrounding carry out repairing clean.
6, bleach:
Separate with nylon wire between each section during bleaching and fix, preventing tissue from losing or displacement, then bleach.With the H of 12% during bleaching 2o 2bleach about one week, guarantee deep layer bleaching effect.Extravasated blood and stomach content are cleaned out after having bleached, clear water rinses two days.
7, dewater:
Strictly control dehydration temperaturre during dehydration-20 DEG C of dehydrations, acetone concentration, changes an acetone in 3 days from low to high, when acetone concentration reaches more than 98%, illustrates that dehydration completes.Should degreasing 1 week in the acetone of normal temperature.Dehydration, degreasing are not thorough, and finished product can be caused to occur floccus or water wave.
8, infiltrate:
During infiltration, pressure should carry out aspirating (pressure limit-0.04 ~-0.08MPa) from low to high, according to resin surface bubbles volume determination pressure, becomes translucent and surperficial bubble-free discharge and can stop to tissue.
9, contact pin lay nameplate
The rear section that infiltrated takes out and carries out acupuncture treatment sign.Pin and nameplate are medical stainless steel material, nameplate carries out paint baking at quarter before the title of acupuncture point, select the paint vehicle immiscible with resin, then engraving cutting is carried out, nameplate can comprise Chinese, international code etc., also as required nameplate can be made different colours, have an acupuncture treatment after acupuncture needle and nameplate have welded, the item (see figure 2) such as orientation of nameplate when strictly getting hold of inserting needle direction, the degree of depth and normally put.
10, die-filling, embedding:
Had an acupuncture treatment can be die-filling after confirmation, if nameplate is in resin, first section is put into mould time die-filling and add epoxy resin embedding agent, section can embed with epoxy resin and be as the criterion by embedding dosage completely that add.Again by each locations of structures confirmation, then mould is adjusted to desired thickness.If make nameplate around section, do not have an acupuncture treatment, to take out after waiting resin to become gel state before die-filling, acupuncture treatment indicates.
11, solidify:
The section that die-filling, embedding have been carried out is put into constant temperature roaster together with mould, temperature is set in 45 degrees Celsius, within 3 hours, solidifies completely.After having solidified, mould is taken out together with section, naturally cool to room temperature and can open mould taking-up section.
12, finishing and installation are shown:
The sample solidified takes out edge trimming is level and smooth and carries out polishing.The sample completed installs runing rest display (see figure 1).

Claims (4)

1. the method for making of the transparent embedding cross-section specimen of human acupoint, it is characterized in that, select to meet normal human's sample that Asian plants physiometry standard, after lavation, conventional preservative treatment, according to normal human's anatomy body surface symbol and Shu Points orientation related assays data, body surface location carried out to the acupuncture points on the human body that will show and penetrates colored plastic pin as mark at the particular location at corresponding acupuncture point, then carrying out freezing, sawing, bleaching, dehydration, infiltration, contact pin lay nameplate, die-filling, embedding and solidification;
According to anatomy and science of acupuncture and moxibustion teaching needs during sawing, sawing becomes different orientation and different thickness;
Pressure limit-0.04 ~-0.08MPa during infiltration, stops when tissue becomes translucent.
2. the method for making of the transparent embedding cross-section specimen of human acupoint according to claim 1, is characterized in that, select the H of mass concentration 10-15% during bleaching 2o 2.
3. the method for making of the transparent embedding cross-section specimen of human acupoint according to claim 1, is characterized in that, the pin that described contact pin laying is selected in nameplate process and nameplate are medical stainless steel material.
4. the method for making of the transparent embedding cross-section specimen of human acupoint according to claim 3, it is characterized in that, nameplate is embedded in resin plane according to demand or is free on around section.
CN201310070913.6A 2013-03-06 2013-03-06 The method for making of the transparent embedding cross-section specimen of a kind of human acupoint Active CN103165009B (en)

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CN104077948B (en) * 2014-07-23 2016-07-13 首都医科大学附属北京佑安医院 Three-dimension disclocation scientific research based on acquired immune deficiency syndrome (AIDS) and method for preparing teaching specimen
CN106157773A (en) * 2015-03-25 2016-11-23 青岛科艺生物技术有限公司 A kind of human body the fourteen meridians follows through Point anatomy section contact pin specimen embedding processing technology
CN108922356A (en) * 2018-07-13 2018-11-30 河南中博科技有限公司 Human body is plasticized nursing teaching sample and preparation method thereof
CN109523873B (en) * 2019-01-22 2021-06-29 河南中博科技有限公司 Soft-covered section specimen and manufacturing method thereof
CN114946827A (en) * 2021-03-08 2022-08-30 郑州卫生健康职业学院 Method for manufacturing animal meridian acupoint section display specimen
CN115005196A (en) * 2021-03-22 2022-09-06 河南中博科技有限公司 Manufacturing method of small-sized mammal meridian acupoint section embedding specimen

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