CN1271928C - Plasticized section of biological tissue and production method thereof - Google Patents
Plasticized section of biological tissue and production method thereof Download PDFInfo
- Publication number
- CN1271928C CN1271928C CN 03134109 CN03134109A CN1271928C CN 1271928 C CN1271928 C CN 1271928C CN 03134109 CN03134109 CN 03134109 CN 03134109 A CN03134109 A CN 03134109A CN 1271928 C CN1271928 C CN 1271928C
- Authority
- CN
- China
- Prior art keywords
- biological tissue
- resin
- section
- lucite
- mould
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a biological tissue plasticized slice and a production method. A biological tissue slice is preprocessed, put in resin liquid or colloid and soaked, the biological tissue slice soaked with resin is put in a mold with an elastic U-shaped cushion clamped between two flat plates, and resin is filled in the mold; the biological tissue slice soaked with resin and the mold filled with resin are put in a water chamber, heated and solidified, and after the resin is polymerized and solidified, the mold is opened; the solidified resin slice is taken out and plasticized into the integrated biological tissue slice; in this way, the transparent high-quality plasticized slice as a finished product fixing the periphery and both sides and tissue of the biological tissue slice can be produced at high speed. The advantages of no constriction, no deformation and no damage are maintained between the biological tissue slice and the tissue.
Description
Technical field: biological tissue section and production method are the resins that the biological tissue section after the dehydration is immersed support fixation, and formation keeps the resin structure of biological tissue section, be the preservation of biological tissue section, be convenient to the medical matters of biological tissue's in-house research observation or the method for teaching product and production.
Background technology: biological tissue section is preserved, all be to cut into slices after biological tissue such as rabbit or the cryonics, again section is put into dipped into formalin or is dried to stem body, also have biological tissue section is immersed resin, behind the resin solidification biological tissue section is fixed.Such section is not supported and is easy to distortion, is not easy to the understanding of histologic section is observed and research; Also there is section one side to be bonded at cardboard with above-mentioned processing, formation is to the localized immobilization of section, but such fixed structure also has the macrobending distortion, and can only observe a side of section, this side tissue that can observe is not owing to fix distortion and damage between can occurring organizing.The biological tissue section of resin solidification, solidify inhomogeneous in ultraviolet irradiation or electricity baking heating process, cause that easily biological tissue and resin each several part color and transparency differ, cause the distortion of biological tissue section easily, also occur the implosion or the local implosion of polymerizing curable process easily, yield rate is low.
Summary of the invention: purpose of the present invention is exactly the technical problem that will solve the fixation of tissue structure of biological tissue section and effectively preserve, provide a kind of fixed biological tissue section indeformable, be convenient to the biological tissue section of organization internal observational study analysis again and be convenient to the plasticizing biological tissue section production method of preserving and using.
Biological tissue of the present invention plasticizing section production method, be with the biological tissue section preliminary treatment, putting into resin liquid or colloid soaks into, the biological tissue section that has soaked into resin is put into the mould that two flat boards clip the resilient U-shaped pad, potting resin in mould, the resin of filling and the biological tissue section that has soaked into resin and mould put in the water tank be heating and curing, open mould after resin polymerization solidifies and take out resin sheet and the plasticizing all-in-one-piece biological tissue section that solidifies.So just produced transparent plasticized section finished product fixing between around the biological tissue section and both sides and the tissue.The resin of filling and the biological tissue section that has soaked into resin and mould put in the water tank be heating and curing, not only can Fast Heating, the heat exchange efficiency height, the uniformity that assurance is heating and curing, and can effectively control the process of polymerizing curable, the heat of polymerization in time can also be discharged and prevent implode, improve the quality of producing product effectively, quality problems can therefore not occur.Resin can obtain uniform heating with the biological tissue section and the mould that have soaked into resin in water tank, can also be in the other direction after polymerization begins the heat of polymerization be transmitted in the water, utilize the polymerization that is heating and curing of the fast heat of polymerization slow, automatically the speed of control polymerizing curable has effectively prevented implode.Resin is preferably lucite resin (methymethacrylate), is aggregated to the colloid shape that can flow naturally under cooling or the normal temperature behind the colloid shape that preferably lucite resin raw material heated polymerizable become can flow, and fills in mould again.Can shorten like this and make the production time, lucite resin transparent degree height, main is that lucite is difficult for reacting the destruction that causes biological tissue with biological tissue.In mould, behind the potting resin, be heating and curing, effectively prevent the monomer volatilization of raw material, help so arbitrarily preventing and the random water that is heated mould and stirs heating that moves filling uncovered sealing on the mould, putting in the water tank again; Preferably stick with glue paper or gluing plastic tape with filling uncovered sealing on the mould, convenient to operation.Place 50 ± 3 ℃ of water baths (pot) to be cured in 2-5 hour on mould, more help can not destroying mechanics of biological tissue again, can also in time discharge the polymerization heat reaching the temperature of polymerizing curable; After the polymerization typing, again water bath (pot) temperature is risen to 90-100 ℃ of heating after 1-3 hour like this, make resin solidify fully fast, just can cool off gradually, can obtain best lucite plasticizing biologic slice product.Structure Biological Tissue SectionKept of the present invention, the biological tissue section that dehydration is arranged, it is characterized in that: biological tissue's epidermis of biological tissue section is connected with the lucite sheet, the gap is filled with the lucite body between the tissue of biological tissue section, and the biological tissue section both side surface is the lucite smooth surface.Be connected with the lucite sheet at biological tissue's epidermis like this and be equivalent to framework, with around the biological tissue section fixedly maintenance do not shrink, indeformable.Be filled in lucite body between tissue supporting indeformable between the tissue, do not damage.The lucite smooth surface of section both side surface and the machine vitreum of tissue space fuse, and be connected with the lucite sheet with the fabric texture epidermis and fuse, promptly be convenient to observe directly the interior of section, can keep the both side surface of cutting into slices again by smooth lucite surface.
The present invention compared with the prior art, have biological tissue section and plastify evenly rapid shaping again, guaranteed the quality of the biological tissue section of plasticizing effectively, make that biological tissue inside is indeformable at the solidification process that is heating and curing, be not destroyed, solidify and implosion or local implosion can not occur, particularly lucite resin transparent degree is good to have guaranteed plasticized resin, is convenient to observe the organization internal structure.The plasticizing biological tissue section that forms not only to biological tissue section profile support fixation, can also carry out support fixation between the institutional framework to section, and form the smooth observation surface of section both sides, is more convenient for observing measuring and researchs and analyses.
Description of drawings: accompanying drawing has represented that a kind of structural representation and manufacturing process that has plasticizing storage configuration brain plasticizing section puts into die perfusion lucite colloid schematic diagram, wherein Fig. 1 cuts into slices for cerebral tissue and puts into the schematic diagram of die perfusion lucite colloid process, and Fig. 2 is for having the brain plasticizing piece cutting structure schematic diagram of storage configuration after plastifying.Case making process and structure below in conjunction with accompanying drawing further specify the present invention.
Concrete fact Example:
Embodiment one, a kind of brain plasticizing section and production method that plastifies storage configuration that have.
The preliminary treatment of brain sections comprises: section, fixing, color keeps or dyeing, dehydration.The brain sample will be cut into slices under freezing state, the best when being lower than-20 ℃.Biological tissue with uniform rigidity uses rotary microtome to cut into slices under freezing state or under the stationary state.Section can use existing treatment fluid (as formalin, glutaraldehyde) to handle.Dyeing: the natural true qualities of section can be preserved by adding sodium nitrate or ascorbic acid in fixer.Can use existing organic material staining technique during dyeing.Therefore the dehydration of brain sections, because the operable lucite of this invention is not miscible with water, the moisture in the section must be with cementing out with the miscible intermediate medium of lucite before the plasticizing.Dehydration can be used gradient alcohol dehydration (concentration gradient is 50%--96%), puts into acetone at last.The not lucite solution of plasticizing is put in the cerebral tissue section of dehydration.Get two flat boards, preferably clean firm sheet glass.Put into the U-shaped packing ring that elastic material is made between two sheet glass, use clip to clamp two sheet glass.So just become a smooth perfusion compartment, formed mould.Packing ring is preferably made by smooth polyvinyl chloride, and its thickness is identical with the thickness of histologic section.Then treated brain sections is put between two sheet glass, the lucite body that is contained between the brain sections tissue is supporting biopsy tissues, solidifies the back and just forms the supporter that is connected with the section both sides; Equally, glue multiple lucite and just formed continuous smooth surface on the section two sides.Put into the funnel of a particular design in the groove above perfusion compartment.The erectility that keeps perfusion compartment is annotated uncured lucite liquid in the groove of perfusion compartment, mainly be the frame body that forms the brain sections periphery, has just formed the rectangle solid frame 2 that is connected with section appearance and both sides after the curing.Opening with elastic material bar closed mould upper end.The lucite resin of filling and the biological tissue section that has soaked into the lucite resin and mould put in the water tank be heating and curing.Place 53 ℃ of water tanks (pot) to carry out 2.3 hours curing on mould, the water heating with water tank makes temperature rise to 95 ℃ again, keeps the cooling gradually after 3 hours of corresponding temperature, just can obtain best lucite plasticizing biologic slice product.Open mould after resin polymerization solidifies and take out resin sheet and the plasticizing all-in-one-piece biological tissue section that solidifies.Remove clip, open perfusion compartment.A brain plasticizing section storage configuration has just been made.Brain sections 1 is positioned at the middle part, lucite body 3 is being filled in gap between the brain sections tissue, the lucite body of filling all is connected with the smooth surface of brain sections both sides, the brain sections exocuticle is connected with organic glass frame 2 around it, and organic glass frame also fuses with the smooth surface 4 of brain sections both sides.Also can repair the frame shape of plasticizing section as required, or in section, add mark for teaching purpose.
Embodiment two, a kind of human body shank knee joint portion of tissue plasticizing section and preparation method that has storage configuration.Useful uncured lucite raw material soaks the human body shank knee joint portion of tissue section of dehydration.The lucite colloid that is in solidification process is filled in gap between this knee joint portion of tissue biopsy tissues.The knee joint portion of tissue section of filling organic glass cement body is clipped between two blocks of glass, accompanies between two glass plate bottoms and the dual-side
The rubber strip of shape.Between two blocks of glass the skin of knee joint portion of tissue section and up and down the section outside pour into the lucite colloid that is in solidification process.In mould, behind the potting resin, stick with glue paper or gluing plastic tape and will fill uncovered sealing on the mould.Place 50 ℃ of water tanks (pot) to carry out 3.5 hours curing on mould, make temperature rise to 90-100 ℃ the heating of the water of water tank again, keep corresponding temperature water temperature of cooling water tank gradually after 2 hours, just can obtain best lucite and plastify the biologic slice product.After the heating cure, be connected with the lucite sheet around the section of knee joint portion of tissue, the lucite of knee joint portion of tissue section both side surface and the gap positive lucite colloid surface of filling between the tissue solidify to form at the glass face and are the lucite smooth surface.The lucite smooth surface of section both side surface and the machine vitreum of tissue space fuse, and with skin, block the outer lucite sheet of layer and fuse, the lucite that is connected with skin is equivalent to framework like this, with knee joint portion of tissue section periphery fixedly maintenance do not shrink, indeformable.Be filled in lucite body between tissue supporting indeformable between the tissue, do not damage.Promptly be convenient to observe directly the interior of knee joint portion of tissue section, can keep knee joint portion of tissue section both side surface not damage again by smooth lucite surface.Be more convenient for observation understanding, test analysis and the research of knee joint portion of tissue structure are judged.
Claims (7)
1, the production method of a kind of biological tissue plasticizing section, with the biological tissue section preliminary treatment, liquid or the colloid of putting into resin soak into, the biological tissue section that has soaked into resin is put into the mould that two flat boards clip the resilient U-shaped pad, potting resin in mould, the resin of filling and the biological tissue section that has soaked into resin and mould put in the water tank be heating and curing, open mould after resin polymerization solidifies and take out resin sheet and the plasticizing all-in-one-piece biological tissue section that solidifies, it is characterized in that: resin is the lucite resin, lucite resin raw material heated polymerizable become to flow behind the colloid shape is aggregated to the colloid shape that can flow under cooling or the normal temperature naturally, fills in mould again.
2, as the production method of the said biological tissue of claim 1 plasticizing section, it is characterized in that: in mould, behind the potting resin, will fill uncovered sealing on the mould.
3, as the production method of the said biological tissue of claim 1 plasticizing section, it is characterized in that: stick with glue paper or gluing plastic tape and will fill uncovered sealing on the mould.
4, as the production method of the said biological tissue of claim 1 plasticizing section, it is characterized in that: mould is placed be cured in 50 ± 3 ℃ of water bath 2-5 hours, again the water bath temperature is risen to 90-100 ℃ of heating cooling gradually after 1-3 hour.
5, a kind of biological tissue plasticizing section, the biological tissue section that dehydration is arranged, it is characterized in that: biological tissue's epidermis of biological tissue section is connected with lucite sheet framework, the gap is filled with the lucite body between the tissue of biological tissue section, and the biological tissue section both side surface is the lucite smooth surface that is connected with organic glass frame with the lucite body.
6, as the plasticizing section of the said biological tissue of claim 5, it is characterized in that: lucite sheet framework outside is circle or ellipse or rhombus or rectangle.
7, as the plasticizing section of the said biological tissue of claim 5, it is characterized in that: lucite sheet framework outside is the framework of biological tissue section profile.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03134109 CN1271928C (en) | 2003-08-15 | 2003-08-15 | Plasticized section of biological tissue and production method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03134109 CN1271928C (en) | 2003-08-15 | 2003-08-15 | Plasticized section of biological tissue and production method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1579155A CN1579155A (en) | 2005-02-16 |
| CN1271928C true CN1271928C (en) | 2006-08-30 |
Family
ID=34579064
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03134109 Expired - Lifetime CN1271928C (en) | 2003-08-15 | 2003-08-15 | Plasticized section of biological tissue and production method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1271928C (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103175717A (en) * | 2011-12-22 | 2013-06-26 | 大连鸿峰生物科技有限公司 | Human tissue acupoint section and production method thereof |
| CN102798540B (en) * | 2012-07-20 | 2014-03-05 | 同济大学 | Method for quickly and stably picking fragile tissue of animal for experiments |
| CN111238911A (en) * | 2020-02-10 | 2020-06-05 | 大连医科大学 | Special perpendicular embedding case of fault plastify |
| CN111337319A (en) * | 2020-02-13 | 2020-06-26 | 大连医科大学 | Water bath device for fault plasticized specimen and water bath hardening method thereof |
-
2003
- 2003-08-15 CN CN 03134109 patent/CN1271928C/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CN1579155A (en) | 2005-02-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4320157A (en) | Method for preserving large sections of biological tissue with polymers | |
| CN1100256C (en) | Method of fixedly supporting biopsy specimen, fixedly supporting agent, and embedding cassette | |
| CN105797216B (en) | A method of porous material is prepared with silk fibroin protein and carboxymethyl chitosan | |
| CN1271928C (en) | Plasticized section of biological tissue and production method thereof | |
| CN107398183A (en) | A kind of glue sealing method of curtain hollow-fibre membrane | |
| JPH02174930A (en) | Preparation of polyvinyl alcohol hydrogel and mri phantom | |
| CN105666801A (en) | Sandwich tablet sample preparing die for epoxy resin and method | |
| DE60216438D1 (en) | A method of molding a composite article and apparatus for carrying out this method | |
| CN103175717A (en) | Human tissue acupoint section and production method thereof | |
| CN211415975U (en) | Silicon rubber filling and sealing mold for sealing and isolating | |
| CN103053510A (en) | Simple manufacturing method for long-term preserving softened human anatomy viscous specimen | |
| CN210061732U (en) | Curing tool for polymer adhesive film | |
| CN202878610U (en) | Plate slide specimen wax-impregnation reverse mold | |
| CN2782023Y (en) | Storage structure for biotic tissure section | |
| WO2022017482A1 (en) | Tissue slicing method and forming device for tissue slicing | |
| CN205021938U (en) | Medical treatment and health protection medicine bottle injection molding machine layering cooling device | |
| ES8601003A1 (en) | Method for making plastified wood | |
| CN111951654A (en) | Method and device for manufacturing skin specimen applied to marine mammal | |
| CN219391532U (en) | Embedding mould for manufacturing pathological paraffin section | |
| CN110006721A (en) | The slice preparation method of kadsura root | |
| GB2082041A (en) | Method for preserving large sections of biological tissue with polymers | |
| RU2689950C1 (en) | Method for preserving bone anatomical preparations | |
| CN217803161U (en) | Injection mold with external cooling system | |
| CN217916617U (en) | Quick fashioned liquid silica gel rubber coating mould | |
| CN116171975A (en) | Manufacturing method of insect crystal glue dripping specimen |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: DALIAN HOFFEN BIO-TECHNIQUE Co.,Ltd. Assignor: Sui Hongjin Contract fulfillment period: 2008.11.27 to 2023.8.14 Contract record no.: 2008210000076 Denomination of invention: Plasticized section of biological tissue and production method thereof Granted publication date: 20060830 License type: Exclusive license Record date: 20081128 |
|
| LIC | Patent licence contract for exploitation submitted for record |
Free format text: EXCLUSIVE LICENSE; TIME LIMIT OF IMPLEMENTING CONTACT: 2008.11.27 TO 2023.8.14; CHANGE OF CONTRACT Name of requester: DALIAN HONGFENG BIOLOGY SCIENCE CO., LTD. Effective date: 20081128 |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20221116 Address after: No. 36, Guangyuan Street, Lvshun Economic Development Zone, Dalian, Liaoning 116000 Patentee after: DALIAN HOFFEN BIO-TECHNIQUE Co.,Ltd. Address before: 465 Department of anatomy, Zhongshan Road 116027, Shahekou District, Liaoning, Dalian Patentee before: Sui Hongjin |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20060830 |