CN103175717A - Human tissue acupoint section and production method thereof - Google Patents
Human tissue acupoint section and production method thereof Download PDFInfo
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- CN103175717A CN103175717A CN2012101039118A CN201210103911A CN103175717A CN 103175717 A CN103175717 A CN 103175717A CN 2012101039118 A CN2012101039118 A CN 2012101039118A CN 201210103911 A CN201210103911 A CN 201210103911A CN 103175717 A CN103175717 A CN 103175717A
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Abstract
The invention discloses a human tissue acupoint section and a production method thereof. The method comprises the following steps of: positioning a sample acupoint, and signing the acupoint, preparing the section, marking the acupoint, bleaching, dehydrating and degreasing the section; acupuncturing the acupoint; embedding and curing the section, and finally splitting the section to obtain the human tissue acupoint section. The tissue section with an acupuncture needle for acupuncturing to mark the name of the acupoint is placed between two glass clips, the resin is filled in the glass clips, the filled resin, the human tissue section infiltrated with the resin and the glass clips are placed in a water bath tank to be heated and cured, after the resin is polymerized and cured, the cured resin sheet and plasticized and integrated human tissue section with the acupuncture needle for marking name of the acupoint are taken out by opening the glass clips. A brand new sample for learning the acupoint positions and distribution relation and a sample manufacturing form are provided, and extreme high value is realized for the medical teaching and research.
Description
Technical field
The present invention relates to the preparation of specimen of corpse local organization, more particularly, relate to the section of tissue acupuncture points on the human body and making thereof.
Background technology
Conventional human acupuncture point model can only be showed the position distribution of different acupuncture points on the human bodys on human body, can not show the position relationship of Different Acupoints on same level.
Summary of the invention
Purpose of the present invention is exactly will solve how the position relationship of the Different Acupoints on same level to be showed intuitively, a kind of brand-new study acupuncture points on the human body position and the sample of distribution relation and the method for making thereof are provided, have had high value for medical teaching and research.
In order to achieve the above object, the production method of a kind of tissue acupuncture points on the human body section of the present invention comprises the steps:
Step 1, sample acupuncture points on the human body are located, and are marked acupuncture points on the human body;
Step 2, preparation section: through freezing sample sawing, the thickness 1-3mm of section guarantees that some acupuncture points on the human body remains on the section same level;
Step 3, indicate the acupuncture point: after the cleaning section, will indicate the label anchoring of acupuncture point title with sewing needle;
Step 4, section bleaching;
Step 5, section dehydration, degreasing;
Step 6, acupuncture points on the human body acupuncture, acupuncture needle are imbedded in the section myofilament, and wherein, needle point and the needle body expose;
Step 7, resin mixture liquor embedding: section is placed in two sheet glass intermediate plates, fills the colloidal resin that flows in the glass intermediate plate, vacuumize, make resin be impregnated into section fully;
Step 8, section are solidified: the acupuncture points on the human body section that will exhaust vacuum is put into water bath and is hardened;
Step 9, tear sheet open: open after curing that the glass intermediate plate takes out the resin sheet that solidifies and plasticizing is integral and with the tissue section that indicates acupuncture points on the human body title acupuncture needle.
Under the optimum seeking method mode, in Step 1, the method for mark acupuncture points on the human body is: rotate gently on skin with 5 millimeters hole making drills of round diameter and leave a trace, then coat color to mark with paintbrush.
The optimal way of Step 1-2: after marking acupuncture points on the human body, first sample is divided into head, trunk after the some parts of four limbs trick, more embedded through gas-development agent, is inserted the freezing above time in week in the refrigerator-freezer of-70 ℃, and then sawing.Can reduce like this requirement to the refrigerator-freezer volume, and be conducive to realize fast freezing.Herein, " some parts " be intended to illustrate and as required sample resolved into required part, as head, and trunk, four limbs trick, and other required parts.And " more than the freezing week ", freezing at least week in other words, the longer time also can, can guarantee the freezing effect of sample.And-70 ℃ of nonessential temperature according to this area experience, determine that freezing temperature gets final product.
And the meaning of " guaranteeing that some acupuncture points on the human body remains on the section same level " in Step 2 is: on the section same level, and for medical usage, a plurality of acupuncture points on the human bodys in section in should identifying local location.Various sections are different parts, therefore the acupuncture points on the human body title in a section is different, but in one plane should indicate a plurality of acupuncture points on the human bodys simultaneously.
In addition, the hydrogen peroxide with 5% in Step 4 is bleached, and bleaching time is 3 days.
And in Step 5, the method for section dehydration is: carry out twice dehydration in the acetone soln of section under-25 ℃ and-15 ℃ and process; The acetone concentration of dewatering for the first time is not too high, is advisable in 85% left and right, and refrigerator temperature is-25 ℃, and dewatering time stabilizes to 5 days.Dehydration for the second time, refrigerator temperature-15 ℃, acetone concentration is to be advisable in 90% left and right, dewatering time stabilizes to 5 days.
The method of section degreasing is: carry out degreasing in acetone soln at normal temperatures, divide 2-3 time with the variable concentrations degreasing; Degreasing for the first time, acetone concentration are to be advisable in 95% left and right, stablize 5 days.Degreasing for the second time, acetone concentration are to be advisable in 97% left and right, stablize 5 days.Degreasing for the third time, acetone concentration is 99.9%, stablizes a week, measures three times, last acetone concentration is not less than 99.7%.。
In addition, in Step 7, for larger section, as the section of torso portion and head, preferred investment: first section is placed on glassly, then makes the glass intermediate plate, at last resin is poured in the glass intermediate plate.And for little section, as the section of upper and lower extremities and hand, pin, preferred completion method: first make the glass intermediate plate, resin is poured in the glass intermediate plate, then the glass intermediate plate is put in section.And under optimum way, in Step 7, be evacuated to negative pressure to reach-during 0.1 MPa till, make resin be impregnated into section fully.
Under optimal way, Step 8 section curing are divided into preliminary sclerosis and harden two processes fully.During preliminary sclerosis, temperature is between 25 ℃-30 ℃; Sclerosis is that the water bath temperature is increased in 40 ℃ fully, hardens 72 hours.Can guarantee solidification effect like this.
The above-mentioned resin used of the present invention is the organic glass resin; During filling, organic glass resin raw material heated polymerizable is become to flow is aggregated to the colloidal that can flow under cooling after colloid or normal temperature naturally, then fills in mould.
According to said method, the present invention also provides a kind of tissue acupuncture points on the human body section, it constitutes: biological tissue's epidermis of histotomy is connected with organic glass sheet framework, the gap of organizing of histotomy is filled with the organic glass body, the histotomy both side surface is the organic glass smooth surface that the organic glass body is connected with organic glass frame, and accompanies the acupuncture needle that each acupuncture points on the human body acupuncture indicates the acupuncture points on the human body title in organic glass.Herein, organic glass sheet framework and organic glass body are all by consisting of after the organic glass resin solidification.
According to said method, the acupuncture points on the human body section of inventor soma, organic glass sheet framework outside is the framework of tissue section profile; And rounded or the oval or rectangle in described organic glass sheet framework outside.
The acupuncture points on the human body section of inventor soma and production method are that fixing resin is supported in immersion after the acupuncture needle of the section of the tissue after dehydration, degreasing acupuncture sign acupuncture points on the human body title, and form to keep the resin structure of the section of tissue and acupuncture points on the human body position, be convenient to the human acupoint position is determined and the medical matters studied or the method for teaching product and production thereof.
The method for making of the acupuncture points on the human body sample that the present invention is brand-new shows the position relationship of Different Acupoints on same tangent plane.The tissue section is anticipated, carry out the acupuncture of acupuncture points on the human body after dehydration, degreasing, the histotomy that acupuncture is indicated acupuncture points on the human body title acupuncture needle is put into two sheet glass intermediate plates, potting resin in the glass intermediate plate, the resin of filling and the acupuncture that infiltrated resin are indicated the tissue section of acupuncture points on the human body title acupuncture needle and glass intermediate plate put in water bath and be heating and curing, open after resin polymerization solidifies that glass clamp takes out the resin sheet that solidifies and plasticizing is integral and with the tissue section that indicates acupuncture points on the human body title acupuncture needle.This invention provides a kind of brand-new study acupuncture points on the human body position and sample and preparation of specimen's mode thereof of distribution relation, has high value for medical teaching and research.
Description of drawings
Fig. 1 is the left hand acupuncture points on the human body section picture in kind of making according to the inventive method;
Fig. 2 is the head acupuncture points on the human body section picture in kind of making according to the inventive method.
Embodiment
Case making process below in conjunction with accompanying drawing further illustrates the present invention.
The production method of inventor soma acupuncture points on the human body section comprises the steps:
1, sample acupuncture points on the human body location.From lung meridian, press bone degree sense of propriety localization method, point location by natural landmarks, automoasurement principle of point selection acumoxioustion are rule and are decided the cave, rotate gently on skin with 5 millimeters hole making drills of round diameter and leave a trace, then coat color to mark with paintbrush.Whole body decide the cave complete after confirmation, sample is carried out office separates, be divided into head, trunk, several parts such as four limbs trick.
2, the sample that gas-development agent is embedded is put into freezing week above time, the then sawing of refrigerator-freezer of-70 ℃.
3, select large-scale band saw to cut.Sample is different, selects the saw blade of different model.As upper limbs, lower limb, first-class sample, can select the medium size saw blade; Torso portion can be selected the large size saw blade.The thickness of normal section is not more than 3mm.Observe at any time the acupuncture point in sawing process, guarantee that some acupuncture points on the human body is maintained at the same horizontal plane.
4, cleaning section is carried out the acupuncture point and is fastened board after cleaning up, one, an acupuncture point board, the acupuncture point is engraved on the plastics board, then with stitch bite of the needle on the acupuncture point of reserving.The left and right limb indicates clear on board.
5, bleaching.Hydrogen peroxide with 5% is bleached, and bleaching time is about 3 days, then prepares dewatering and defatting.
6, carry out processed in the acetone soln of section under-25 ℃ and-15 ℃.Dehydration for the first time, refrigerator temperature is-25 ℃, and acetone concentration is not too high, and dewatering time stabilizes to 5 days.Dehydration for the second time, refrigerator temperature-15 ℃, dewatering time stabilizes to 5 days.
7, carry out degreasing in section acetone soln at normal temperatures.Generally divide 2-3 variable concentrations degreasing.
8, the acupuncture points on the human body acupuncture is carried out in the section that will complete dehydration, ungrease treatment, the plastics acupuncture points board that contrasting marking is good is found the acupuncture points on the human body position accurately and is carried out acupuncture, the acupuncture needle that indicates the acupuncture points on the human body title is not embedded in the section myofilament entirely, and it is out exposed that needle point and the needle body are partly wanted, in order to watch.
9, the section embedding is divided into two kinds of investment and completion methods.Investment is first section to be placed on glassly, then makes the glass intermediate plate, at last the organic glass resin mixture liquor is poured in the glass intermediate plate.General this kind method is made larger section, as torso portion and head section.Completion method is first to make the glass intermediate plate, the organic glass resin mixture liquor is poured in the glass intermediate plate, then the glass intermediate plate is put in section.This kind method is general makes little section, as the section of upper and lower extremities and hand, pin.
10, section vacuumizes.The organic glass resin mixture liquor is infiltrated fully under negative pressure state, negative pressure reaches-during 0.1 MPa till.
The acupuncture points on the human body section that 11, will exhaust vacuum is put into water bath and is hardened, and 40 ℃ of water baths are put in section, and hardening after 72 hours is removable.
Aforementioned production method, the tissue section is anticipated, carry out the acupuncture of acupuncture points on the human body after dehydration, degreasing, the histotomy that acupuncture is indicated acupuncture points on the human body title acupuncture needle is put into two sheet glass intermediate plates, potting resin in the glass intermediate plate, the resin of filling and the acupuncture that infiltrated resin are indicated the tissue section of acupuncture points on the human body title acupuncture needle and glass intermediate plate put in water bath and be heating and curing, open after resin polymerization solidifies that glass clamp takes out the resin sheet that solidifies and plasticizing is integral and with the tissue section that indicates acupuncture points on the human body title acupuncture needle.Method for optimizing and characteristics:
(1) cut into slices each acupuncture points on the human body acupuncture indicates the acupuncture needle of acupuncture points on the human body title; And should indicate in each acupuncture points on the human body acupuncture of pretreated section the acupuncture needle of acupuncture points on the human body title
(2) resin is the organic glass resin, and organic glass resin raw material heated polymerizable is become to flow is aggregated to the colloidal that can flow under cooling after colloid or normal temperature naturally, then fills in mould;
(3) after potting resin, vacuumize in the glass intermediate plate;
The section that (4) will exhaust vacuum is put into water bath and is hardened, and sclerosis is divided into preliminary sclerosis and hardens two processes fully.During preliminary sclerosis temperature between 25 ℃-30 ℃ (different tissues position water temperature different) fully sclerosis be during the water bath temperature is increased to 40 ℃, hardening got final product after 72 hours.
In addition, said method is mentioned, " dewater in the acetone soln in the refrigerator-freezer that the diameter of hole making drill is 5 millimeters ,-70 ℃ under a freezing week above, 5% hydrogen peroxide bleaching approximately 3 days ,-25 ℃ and-15 ℃; the concentration of degreasing and acetone soln etc. in acetone soln ", these technological parameters are determined according to concrete situation of making, be not to necessarily require, these those skilled in the art can reach under each technique purpose prerequisite, arbitrarily the selection technological parameter of effects equivalent.
The tissue acupuncture points on the human body section of the present invention's preparation as illustrated in fig. 1 and 2, biological tissue's epidermis of histotomy is connected with organic glass sheet framework, the gap of organizing of histotomy is filled organic vitreum, the histotomy both side surface is the organic glass smooth surface that is connected with organic glass frame with the organic glass body, and accompanies the acupuncture needle that each acupuncture points on the human body acupuncture indicates the acupuncture points on the human body title in organic glass.The characteristics of inventor soma acupuncture points on the human body section are:
(1) organic glass sheet framework outside is circle or ellipse or rectangle;
(2) organic glass sheet framework outside is the framework of tissue section profile;
(3) each acupuncture points on the human body acupuncture indicates the acupuncture needle of acupuncture points on the human body title.
The above; only be the better embodiment of the present invention; but protection scope of the present invention is not limited to this; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to replacement or changed according to technical scheme of the present invention and inventive concept thereof, within all should being encompassed in protection scope of the present invention.
Claims (12)
1. the production method of a tissue acupuncture points on the human body section, is characterized in that, comprises the steps:
S1, sample acupuncture points on the human body are located, and are marked acupuncture points on the human body;
S2, preparation section: through freezing sample sawing, the thickness 1-3mm of section guarantees that some acupuncture points on the human body remains on the section same level;
S3, indicate the acupuncture point: after the cleaning section, will indicate the label anchoring of acupuncture point title with sewing needle;
S4, section bleaching;
S5, section dehydration, degreasing;
S6, acupuncture points on the human body acupuncture, acupuncture needle are imbedded in the section myofilament, and wherein, needle point and the needle body expose;
S7, resin mixture liquor embedding: section is placed in two sheet glass intermediate plates, fills the colloidal resin that flows in the glass intermediate plate, vacuumize, make resin be impregnated into section fully;
S8, section are solidified: the acupuncture points on the human body section that will exhaust vacuum is put into water bath and is hardened;
S9, tear sheet open: open after curing that the glass intermediate plate takes out the resin sheet that solidifies and plasticizing is integral and with the tissue section that indicates acupuncture points on the human body title acupuncture needle.
2. the production method of tissue acupuncture points on the human body section according to claim 1, is characterized in that, in S1, the method for mark acupuncture points on the human body is: rotate gently on skin with 5 millimeters hole making drills of round diameter and leave a trace, then coat color to mark with paintbrush.
3. the production method of tissue acupuncture points on the human body section according to claim 2, it is characterized in that, among step S1-S2, after marking acupuncture points on the human body, first sample is divided into head, trunk, after the some parts of four limbs trick, embedded through gas-development agent again, insert the freezing above time in week in the refrigerator-freezer of-70 ℃, and then sawing.
4. the production method of tissue acupuncture points on the human body section according to claim 3, is characterized in that, the hydrogen peroxide with 5% in S4 is bleached, and bleaching time is 3 days.
5. the production method of tissue acupuncture points on the human body section according to claim 4, is characterized in that, in S5, the method for section dehydration is: carry out twice dehydration in the acetone soln of section under-25 ℃ and-15 ℃ and process; Dehydration for the first time, refrigerator temperature is-25 ℃, and acetone concentration is 85%, and dewatering time stabilizes to 5 days; Dehydration for the second time, refrigerator temperature-15 ℃, acetone concentration is 90%, dewatering time stabilizes to 5 days;
The method of section degreasing is: carry out degreasing in acetone soln at normal temperatures, divide 2-3 time with the variable concentrations degreasing; Degreasing for the first time, acetone concentration is 95%, stablizes 5 days; Degreasing for the second time, acetone concentration is 97%, stablizes 5 days; Degreasing for the third time, acetone concentration is 99.7%-99.9%, stablizes a week.
6. the production method of tissue acupuncture points on the human body section according to claim 5, is characterized in that, in S7, investment is selected in the embedding of section resin mixture liquor: first section is placed on glassly, then makes the glass intermediate plate, at last resin is poured in the glass intermediate plate.
7. the production method of tissue acupuncture points on the human body section according to claim 5, is characterized in that, in S7, completion method is selected in the embedding of section resin mixture liquor: first make the glass intermediate plate, resin is poured in the glass intermediate plate, then the glass intermediate plate is put in section.
8. the production method of according to claim 6 or 7 described tissue acupuncture points on the human bodys sections, is characterized in that, in S7, be evacuated to negative pressure to reach-during 0.1 MPa till, make resin be impregnated into section fully.
9. the production method of according to claim 1-7 arbitrary described tissue acupuncture points on the human body sections, is characterized in that, resin used is the organic glass resin; During filling, organic glass resin raw material heated polymerizable is become to flow is aggregated to the colloidal that can flow under cooling after colloid or normal temperature naturally, then fills in mould.
10. the production method of tissue acupuncture points on the human body section according to claim 9, is characterized in that, the S8 section is solidified and is divided into preliminary sclerosis and hardens two processes fully; During preliminary sclerosis, temperature is between 25 ℃ one 30 ℃; Sclerosis is that the water bath temperature is increased in 40 ℃ fully, hardens 72 hours.
11. tissue acupuncture points on the human body section, it is characterized in that, biological tissue's epidermis of histotomy is connected with organic glass sheet framework, the gap of organizing of histotomy is filled organic vitreum, the histotomy both side surface is the organic glass smooth surface that is connected with organic glass frame with the organic glass body, and accompanies the acupuncture needle that each acupuncture points on the human body acupuncture indicates the acupuncture points on the human body title in organic glass.
12. described tissue acupuncture points on the human body section, is characterized in that according to claim 11, is the framework of tissue section profile outside organic glass sheet framework; And rounded or the oval or rectangle in described organic glass sheet framework outside.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012101039118A CN103175717A (en) | 2011-12-22 | 2012-04-10 | Human tissue acupoint section and production method thereof |
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| CN201110435810.6 | 2011-12-22 | ||
| CN201110435810 | 2011-12-22 | ||
| CN2012101039118A CN103175717A (en) | 2011-12-22 | 2012-04-10 | Human tissue acupoint section and production method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103674677A (en) * | 2013-12-17 | 2014-03-26 | 哈尔滨医科大学 | Preparation method of brain tissue rapid freezing section |
| CN104269095A (en) * | 2014-10-23 | 2015-01-07 | 首都医科大学附属北京佑安医院 | Three-dimensional fault scientific research and teaching specimen preparation method based on HBV background hepatocellular carcinoma |
| CN106157773A (en) * | 2015-03-25 | 2016-11-23 | 青岛科艺生物技术有限公司 | A kind of human body the fourteen meridians follows through Point anatomy section contact pin specimen embedding processing technology |
| CN115005196A (en) * | 2021-03-22 | 2022-09-06 | 河南中博科技有限公司 | Manufacturing method of small-sized mammal meridian acupoint section embedding specimen |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103674677A (en) * | 2013-12-17 | 2014-03-26 | 哈尔滨医科大学 | Preparation method of brain tissue rapid freezing section |
| CN103674677B (en) * | 2013-12-17 | 2016-05-25 | 哈尔滨医科大学 | A kind of preparation method of brain tissue quick frozen-section |
| CN104269095A (en) * | 2014-10-23 | 2015-01-07 | 首都医科大学附属北京佑安医院 | Three-dimensional fault scientific research and teaching specimen preparation method based on HBV background hepatocellular carcinoma |
| CN106157773A (en) * | 2015-03-25 | 2016-11-23 | 青岛科艺生物技术有限公司 | A kind of human body the fourteen meridians follows through Point anatomy section contact pin specimen embedding processing technology |
| CN115005196A (en) * | 2021-03-22 | 2022-09-06 | 河南中博科技有限公司 | Manufacturing method of small-sized mammal meridian acupoint section embedding specimen |
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Application publication date: 20130626 |