CN201685400U - Special mould for preparing tissue engineering tubular supports - Google Patents
Special mould for preparing tissue engineering tubular supports Download PDFInfo
- Publication number
- CN201685400U CN201685400U CN2010201887749U CN201020188774U CN201685400U CN 201685400 U CN201685400 U CN 201685400U CN 2010201887749 U CN2010201887749 U CN 2010201887749U CN 201020188774 U CN201020188774 U CN 201020188774U CN 201685400 U CN201685400 U CN 201685400U
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- tissue engineering
- mould
- die ontology
- orifice plate
- tubular
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Abstract
The utility model discloses a special mould for preparing tissue engineering tubular supports, which comprises a tubular mould body. A filling port and an exhaust end are respectively arranged at two ends of the mould body; a filling port plug is arranged on the filling port; an extending section is arranged at the exhaust end in a covering manner; a vent hole is arranged on the extending section; a vent hole plug is arranged on the vent hole; at least two pore plates are arranged inside the mould body; a fixed hole is arranged in the middle of each pore plate; and a notch for materials to pass through is arranged at the edge in the middle of each pore plate. The mould has the advantages of simple structure, convenient manufacture and use and capability of preparing the tubular tissue engineering supports of various microscopic physical forms by the aid of combination with separation process.
Description
Technical field
The utility model relates to a kind of mould of tissue engineering bracket, particularly a kind of particular manufacturing craft for preparing tissue engineering tubular scaffold.
Background technology
Tissue engineering technique is the New Policy of treatment human body and all kinds of injuries of tissues and organs of animal, pathology.Tissue engineering technique is based on tissue engineering bracket, and complex gene treatment and cell technology are made artificial organ and organ, is used for substituting diseased tissue and organ.
Tissue engineering tubular scaffold is mainly used in the reparation and the reconstruction of tubular tissue and organ, as nerve, tendon, blood vessel, tracheae, urethra etc.The technology of injection or moulding by casting is generally adopted in the preparation of this tubular bracket, also has by the method for cutting, digging to make tubular bracket.Tissue engineering tubular scaffold is except the external morphology of tubulose, and its physical aspect feature also is an important parameter, and the effect of tissue engineering bracket is had fundamental influence.The tissue engineering bracket that preparation has specific physical arrangement need be reference frame with the morphosis of natural fabric, the morphosis parameter of control support on three yardsticks: macroscopic view (cm), submicroscopic (mm) and microcosmic (nm).Different applied environments has different requirements to the inner constructional form of support, and also is not quite similar in the requirement of different yardstick (macroscopical, submicroscopic, microcosmic) to structural form.For example, the tubular construction that cardiovascular tissue engineering bracket adopts typical single passage to connect on macroscopic view usually, tube wall approaches and is micropore closely knit or that have superfine (nm level) on microcosmic, this model has in the tubular tissue organ support of conveying function comparatively common at trachea bracket, urethra rack and other, in the application process, this wall construction closely knit or superfine hole produces when being unlikely to cause material to be carried and leaks.And be used for the support of nerve (on every side or cental system) tissue repair, multichannel conduit can obtain better effect, this conduit is arranged the passage of one or more vertical perforations at columniform body, macroscopic view form and lotus rhizome or honeycomb briquette are similar, and on the submicroscopic-microscopic pattern of the pars intramuralis of channel bracket, generally has micron-sized hole to increase porosity, permeability etc., studies have shown that when these conduit wall inside have the hierarchical porous structure of submicroscopic-microscopic dimensions, be of value to and in support, set up neuroid.
By cutting, dig the method for bulk material being made tubular bracket, the influence that the tubular bracket outer wall construction that makes is produced because of its machinery or Laser Processing means is big and be difficult to assessment, can not satisfy as necessary accurate, the stable requirement of tissue engineering bracket.Adopting cast or injection molding scheme, according to the tissue engineering bracket that designs and produces specific modality, is the most feasible, easy so far method on macro-scale.Yet, tissue engineering technique has very high requirement to the form submicroscopic, micro-scale of support, therefore design a cover technology and a mould, the technology and the injection molding scheme that support are prepared the formation of related submicroscopic, micro-scale form are united, and be very great to the meaning of accurate preparation tissue engineering bracket.
In recent years, some occur and be applied to cast or injection molding mould.But there is significant disadvantages in these moulds: (1) assembling is complicated, uses loaded down with trivial details; (2) the raw-material applicability that the making support is used is not high; (3) process is set up the compatible not enough of submicroscopic, micromorphologic technology to other; (4) external condition (as temperature and amplitude of variation trend and direction thereof etc.) narrow, the poor controllability of accommodation during preparing product, batch difference is difficult to control; (5) efficient of mould own is low, and extensibility is little.
The utility model content
In order to solve the above problems, the purpose of this utility model is to provide a kind of precision die simple and easy, easy to use, that the material range of choice is wide, preparation technology parameter is conveniently adjusted that assembles, they can be according to the needs of all kinds of tissues with conveying function or organ, tubulose outward appearance, all kinds of tissue engineering tubular scaffolds that the preparation macroscopic view has accurate physical arrangement to the microcosmic full size scope.
The technical scheme that the invention for solving the technical problem adopted is:
A kind of particular manufacturing craft that is used to prepare tissue engineering tubular scaffold, comprise die ontology in a tubular form, described die ontology two ends are respectively sprue and exhaust end, described sprue is provided with the sprue plug, described exhaust end is provided with cover extension thereon, described extension is provided with steam vent, described steam vent is provided with the exhaust stopple, be provided with at least two orifice plates in the described die ontology, the middle part of described orifice plate is provided with fixing hole, and the edge at the middle part of described orifice plate is provided with and is used for the breach that material passes through.
As improvement of the technical scheme, be provided with the draw-in groove that is used for fixing orifice plate in the described die ontology.
As further improvement in the technical proposal, described die ontology is fixedly formed by left mould and right die combination.
As further improvement in the technical proposal, described left mould one end is provided with left mould location buckle, and described right mould is provided with the right mould location buckle that matches with left mould location buckle.
As further improvement in the technical proposal, the exhaust end of described die ontology has constant slope, and the part that matches with exhaust end in described extension also has corresponding gradient.
The beneficial effects of the utility model are:
(1) mould structure of preparation tissue engineering tubular scaffold of the present utility model simple, be easy to make and use;
(2) cooperate technology such as be separated to produce to have macroscopic view, the tubular bracket of submicroscopic and microphysics structure;
(3) because the die cavity outside is the uniform tubular of wall thickness, die ontology has isotropism on cross section, to the direction of transfer of the amplitude of the selection of temperature, variations in temperature and trend, heat etc. have higher sensitivity and adaptability, controllability is good, batch difference is little;
(4) inside is provided with edge's orifice plate jaggy, be used for fixing in the distribution, orifice plate of the groove of orifice plate the quantity in hole and breach distributional pattern and orifice plate on the material of each assembly of mould and dimensional parameters, the inner chamber and all can adjust favorable expandability according to application requirements.
(5) mould of preparation tissue engineering tubular scaffold of the present utility model, when being used to prepare the controlled tissue engineering bracket of broad range scale (from cm to nm), structure, can adopt dissimilar pore-foaming agents, as solid phase or liquid phase, water-soluble or (being used different pore-foaming agent removal methods) such as oil-soluble, dissolving or volatilizations; Also can accurately control processing conditions, as the temperature range in injection-forming process, range of temperature and, the direction of transfer of heat etc.Therefore, adopt mould of the present utility model to prepare the tubular tissue engineering rack, in raw-material selection, have higher adaptability.
Description of drawings
The utility model is described in further detail below in conjunction with drawings and Examples:
Fig. 1 is the utility model structural representation;
Fig. 2 is that the utility model splits schematic diagram;
Fig. 3 is an AND DEWATERING FOR ORIFICE STRUCTURE schematic diagram of the present utility model;
Fig. 4 is the utility model extension cross-sectional view.
The specific embodiment
Referring to figs. 1 through Fig. 4, a kind of particular manufacturing craft that is used to prepare tissue engineering tubular scaffold of the present utility model, comprise die ontology 1 in a tubular form, described die ontology 1 two ends are respectively sprue 11 and exhaust end 12, described sprue 11 is provided with sprue plug 2, described exhaust end 12 is provided with cover extension 3 thereon, described extension 3 is provided with steam vent 31, described steam vent 31 is provided with exhaust stopple 4, be provided with at least two orifice plates 5 in the described die ontology 1, the fixing hole 51 that the middle part of described orifice plate 5 is provided with, the edge at the middle part of described orifice plate 5 are provided with and are used for the breach 52 that material passes through.Wherein, fixing hole 51 is used for fixing vertical fiber arranged silk (wire, plastics or inorfil), and its number can be determined according to actual needs, as the single pass tissue engineering bracket of needs, only needs a fixing hole 51 to get final product.And the number of orifice plate 5 and mutual distance also can be determined according to actual conditions, once prepare 3 supports as needs, totally 4 orifice plates then can be set, have between them that totally 3 forming cavities can produce three required supports, and can realize by the distance of regulating between the orifice plate 5 for the control of the length of support.
Wherein, the effect of orifice plate 5 is the filament that fixedly runs through, and the arrangement of filament in cavity body of mould is by the distribution decision of fixing hole 51 on the orifice plate 5, and cross section shapes and size are by the form and the size decision of fixing hole 51 on the orifice plate 5.Macroscopical form of the product that the common decision of filament and die cavity is prepared.The breach 52 of orifice plate 5 edge designs is used for flowing or passing through of material.As when adopting the out-phase pore-foaming agent in conjunction with the submicroscopic morphosis of pore-filtering technology controlling and process conduit, these breach 52 designs can make the out-phase pore-foaming agent be entered each section of die cavity by sprue 11.The effect of extension 3 and steam vent 31 is in the exhaust, knockout course in the injection process removal to the column type pore-foaming agent.Exhaust stopple 4 and sprue plug 2 are used to guarantee the sealing of material injection processing procedure afterwards.
The mould structure of preparation tissue engineering tubular scaffold of the present utility model is simple, be easy to make and use; To raw-material wide adaptability; Be easy in conjunction with technology such as thermic is separated produce have macroscopic view simultaneously, the tubular bracket of submicroscopic and micro-scale hole shape looks structure; Secondly, because the die cavity outside is the uniform tubular of wall thickness, die ontology has isotropism on cross section, to the direction of transfer of the amplitude of the selection of temperature, variations in temperature and trend, heat etc. have higher sensitivity and adaptability, controllability is good, batch difference is little; In addition, inside is provided with edge's orifice plate jaggy, be used for fixing in the distribution, orifice plate of the groove of orifice plate the quantity in hole and breach distributional pattern and orifice plate on the material of each assembly of mould and dimensional parameters, the inner chamber and all can adjust favorable expandability according to application requirements.
For the ease of effective fixedly orifice plate 5,, be provided with the draw-in groove 17 that is used for fixing orifice plate 5 in the described die ontology 1 as preferred implementation.During installation, allow orifice plate 5 be positioned at this draw-in groove 17, can guarantee that in the mould use orifice plate can not be moved.
The easy demoulding of the product of producing in order to guarantee, as preferably, the die ontology 1 described in the present embodiment is fixedly formed by left mould 13 and 14 combinations of right mould.And, for preventing left and right sides mould 13, it is wrong relatively right to take place behind 14 matched moulds, guarantee carrying out smoothly of production, described left mould 13 1 ends are provided with left mould location buckle 15, and described right mould 14 is provided with the right mould location buckle 16 that matches with left mould location buckle 15, when left and right sides mould 13, behind 14 matched moulds, left mould location buckle 15 and right mould location buckle 16 tablings hinder mutual changing of the relative positions trend.In addition, unclamp behind left and right sides mould 13,14 matched moulds, can take some fixation, as can on die ontology 1, being inserted in clip behind its matched moulds in order to make.
In addition, should be noted that die ontology 1 also can be designed as three, or even the combination of polylith mould for the ease of the demoulding, not only be confined in this enforcement about the combination of two moulds.
In order to make that combination is more tight between extension 3 and the die ontology 1, the exhaust end 12 of described die ontology 1 has constant slope, and the part that matches with exhaust end 12 in described extension 3 also has corresponding gradient.Certainly, can also be between extension 3 and the die ontology 1 by being threaded.
Certainly, the utility model is created and is not limited to above-mentioned embodiment, those of ordinary skill in the art also can make equivalent variations or replacement under the prerequisite of the utility model spirit, modification that these are equal to or replacement all are included in the application's claim institute restricted portion.
The utility model is described in further detail below in conjunction with using method:
1, mould assembling
A, orifice plate 5 is individually fixed in the draw-in groove 17 on the die ontology 1, the diameter that inserts appropriate length in the fixing hole 51 of orifice plate 5 is 200 microns the nickel alloy wire with shape memory function, and nickel alloy wire forms in orifice plate 5 and runs through and fixed-site; Form circular tube structure, pipe is fixed, and will be fixed into the assembly insertion extension 3 of pipe, use exhaust stopple 4 to seal steam vent 31 with clip.
B, an amount of sodium acid carbonate microballoon (diameter 75-125 micron) is added in the above-mentioned pipe that assembles by sprue 11, vibration makes microballoon fill up cavity.The breach 52 at orifice plate 5 edges can make the sodium acid carbonate microballoon enter and be full of the cavity of one deck down.
C, (sprue up) just put in the combination of step C gained, to draw an amount of PLLA (PLLA)/DOXANE (dioxane) binary solution (Polymer:DOXANE=6%, wt/v) syringe inserts sprue, inverted die (sprue down), take off exhaust stopple 4, solution is pushed in the mould by syringe.
After D, injection finish, use exhaust stopple 4 seal vents 31, turn mould direction (sprue up) once more, take out syringe, use sprue plug 2 sealing sprues.
2, phase separation
The aforementioned mould that assembles is placed-40 ℃ of permanent low temperature oil bath submergences, and the temperature-fall period polymer solution is separated and finishes.Keep 1h, treat that the mould inside temperature evenly after, take out.
3, the removal of solvent
Remove the grinding tool assembly successively: sprue plug 2, exhaust stopple 4, extension 3; Nickel alloy wire is extracted one by one, freeze drying 72 hours obtains the polymer pipe that inside comprises pore-foaming agent (sodium acid carbonate microballoon) again.
4, open die cavity, remove polymer pipe, place 4 ℃ water 4 days (changing water once in per 6 hours), water is removed DOXANE and the sodium acid carbonate of remnants fully.Freeze drying once more can obtain multichannel tubulose support.
Claims (5)
1. particular manufacturing craft that is used to prepare tissue engineering tubular scaffold, it is characterized in that: comprise die ontology (1) in a tubular form, described die ontology (1) two ends are respectively sprue (11) and exhaust end (12), described sprue (11) is provided with sprue plug (2), described exhaust end (12) is provided with cover extension (3) thereon, described extension (3) is provided with steam vent (31), described steam vent (31) is provided with exhaust stopple (4), be provided with at least two orifice plates (5) in the described die ontology (1), the middle part of described orifice plate (5) is provided with fixing hole (51), and the edge at the middle part of described orifice plate (5) is provided with the breach (52) that is used for material and passes through.
2. the particular manufacturing craft that is used to prepare tissue engineering tubular scaffold according to claim 1 is characterized in that: be provided with the draw-in groove (17) that is used for fixing orifice plate (5) in the described die ontology (1).
3. the particular manufacturing craft that is used to prepare tissue engineering tubular scaffold according to claim 1 and 2 is characterized in that: described die ontology (1) is fixedly formed by left mould (13) and right mould (14) combination.
4. the particular manufacturing craft that is used to prepare tissue engineering tubular scaffold according to claim 3, it is characterized in that: described left mould (13) one ends are provided with left mould location buckle (15), and described right mould (14) is provided with the right mould location buckle (16) that matches with left mould location buckle (15).
5. the particular manufacturing craft that is used to prepare tissue engineering tubular scaffold according to claim 1, it is characterized in that: the exhaust end (12) of described die ontology (1) has constant slope, and described extension (3) also have corresponding gradient with the part that exhaust end (12) matches.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010201887749U CN201685400U (en) | 2010-05-14 | 2010-05-14 | Special mould for preparing tissue engineering tubular supports |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010201887749U CN201685400U (en) | 2010-05-14 | 2010-05-14 | Special mould for preparing tissue engineering tubular supports |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201685400U true CN201685400U (en) | 2010-12-29 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010201887749U Expired - Lifetime CN201685400U (en) | 2010-05-14 | 2010-05-14 | Special mould for preparing tissue engineering tubular supports |
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| Country | Link |
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| CN (1) | CN201685400U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101850600A (en) * | 2010-05-14 | 2010-10-06 | 中山大学 | Manufacturing method and mould of tissue engineering tubular scaffold with hierarchical porous structure |
| CN104382672A (en) * | 2014-11-14 | 2015-03-04 | 东华大学 | Double-layer multichannel nerve conduit and preparation method thereof |
| CN104943042A (en) * | 2015-05-25 | 2015-09-30 | 广州新诚生物科技有限公司 | Method and die for making multichannel nerve conduit |
| CN114393773A (en) * | 2022-01-13 | 2022-04-26 | 辽宁工业大学 | Portable injection mold for manufacturing intravascular stent |
-
2010
- 2010-05-14 CN CN2010201887749U patent/CN201685400U/en not_active Expired - Lifetime
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101850600A (en) * | 2010-05-14 | 2010-10-06 | 中山大学 | Manufacturing method and mould of tissue engineering tubular scaffold with hierarchical porous structure |
| CN101850600B (en) * | 2010-05-14 | 2012-05-23 | 中山大学 | Manufacturing method and mould of tissue engineering tubular scaffold with hierarchical porous structure |
| CN104382672A (en) * | 2014-11-14 | 2015-03-04 | 东华大学 | Double-layer multichannel nerve conduit and preparation method thereof |
| CN104943042A (en) * | 2015-05-25 | 2015-09-30 | 广州新诚生物科技有限公司 | Method and die for making multichannel nerve conduit |
| CN114393773A (en) * | 2022-01-13 | 2022-04-26 | 辽宁工业大学 | Portable injection mold for manufacturing intravascular stent |
| CN114393773B (en) * | 2022-01-13 | 2023-12-12 | 辽宁工业大学 | Portable injection mold for manufacturing vascular stent |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20101229 Effective date of abandoning: 20120523 |