CN1239150C - Nano-scale vitamin microemulsion and preparation method thereof - Google Patents
Nano-scale vitamin microemulsion and preparation method thereof Download PDFInfo
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- CN1239150C CN1239150C CNB2004100062729A CN200410006272A CN1239150C CN 1239150 C CN1239150 C CN 1239150C CN B2004100062729 A CNB2004100062729 A CN B2004100062729A CN 200410006272 A CN200410006272 A CN 200410006272A CN 1239150 C CN1239150 C CN 1239150C
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- vitamin
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- microemulsion
- emulsion
- nanoscale
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Abstract
The invention relates to a vitamin microemulsion with a nano-scale particle size, which is prepared by adding components such as vitamin, an emulsifier, isomaltooligosaccharide and the like into deionized water, crushing the mixture into the nano-vitamin microemulsion by using a high-pressure homogenizer of 70MPa and 150MPa, and measuring the granularity of the nano-vitamin microemulsion to be 19.7-24.8 nm by using a laser light scattering instrument. The nano-scale vitamin greatly improves the solubility, the dispersibility and the dialysis performance of the vitamin in water, changes the physiological and biochemical processes of the vitamin in vivo, eliminates the difference of absorption and utilization among individuals, and has the absorption rate of 100 percent and the bioavailability of more than 90 percent. The nano-scale vitamin microemulsion also solves the problem that the potency of the vitamin is reduced due to the instability of environmental factors, and greatly improves the stability of the vitamin. Can be widely applied to industries such as feed, cosmetics, medicine, beverage, health care products and the like.
Description
Affiliated technical field
The present invention relates to vitamin, more particularly, the present invention relates to a kind of vitamin microemulsion with nanoscale particle diameter.The invention still further relates to the preparation method of this vitamin microemulsion.
Background technology
Vitamin is the low-molecular-weight organic compound of a class biologically active, it neither animal body organize raw material, neither energy substance, but humans and animals is kept the requisite nutrient substance of normal physiological function.Vitamin generally can not synthesize in body, even if can synthesize a spot of water soluble vitamins in the large intestine of body, does not also satisfy the needs of organism metabolism.Most vitamin must be by providing in the food or other external arbitrary way supply.Lack some vitamin in food, when also not having the corresponding in vitro supply simultaneously, body will cause various corresponding avitaminosis.
For replenishing of vitamin, human body mainly is to carry out supply by wholefood, and as vegetable, fruit, meat, newborn class and egg etc., over nearly 20 years, the way of forced for vitamins in the also oriented food is promptly added vitamin in food.The capsule, pill or the oral liquid that have occurred a lot of compound vitamines in recent years again, this is for to the human body vitimin supplement, and the prevention avitaminosis all is useful undoubtedly.
For poultry, mostly all be by in feedstuff, add vitamin or in drinking-water, add support one's family usually satisfy each growth stage of poultry in the demand of vitamin and the livestock products to the requirement of vitamin.
Although these above-mentioned vitamin addition manners are effective, also not exclusively fully up to expectations, its main cause is as follows:
1, because vitamin is very responsive to the reaction of environment on every side such as temperature, humidity, oxidation, reduction, heavy metal ion, light and pH value etc., and between the vitamin, between vitamin and the trace element, between vitamin and other nutrient substance, antagonism in various degree all may take place between vitamin and the carrier, thereby make the reduction of tiring of vitamin.So, although in food and feedstuff, added the vitamin of capacity, clinically still avitaminosis be might show as, and vitaminized food and feedstuff added, because the sensitivity of its vitamin, shelf life is all very short.
2, in the course of processing of food and feedstuff, the loss of tiring of vitamin is very big.Because in process of manufacture, formed the disadvantageous physical environment of vitamin, as mechanicalness damage, high temperature, high pressure and high humidity, the capital makes tiring of vitamin have a greatly reduced quality, so in the production of food and feedstuff, must doubled amount add vitamin, to offset the loss that vitamin is tired in the production process, strengthened production cost.
3, the now domestic and international used vitamin of feedstuff industry all is micron-sized powdered granule, fatsoluble vitamin in the Aquavite also all is emulsive micron order oil droplet, affinity extreme difference with water and gastro-intestinal Fluid, so water solubility is also poor, influenced and fed and drink effect, tired and have only 7～25%, added glucose powder as carrier at Aquavite in addition, long-term drink is stopped up water fountain easily, causes a large amount of harmful microbes to grow.
The fatsoluble vitamin of being strengthened in food, major part are directly to add or be treated as emulsion, because poorly water-soluble causes the vitamin content in the unit product inhomogeneous, have influenced strengthening effect, have reduced product specification.
4, the diversity ratio that absorbs between individuality is bigger.Be each individual health status difference, the degree that absorbs of vitamin also is not quite similar.As fatsoluble vitamin, because its hydrophobicity, it absorbs the same with fat, must dissolve the surface that could see through the liquid environment in the enteric cavity and arrive absorptive cell,intestinal via the bile of hepatic secretion, and the ester of fatsoluble vitamin needs the decomposition through intestinal pancreatic lipase and intestinal mucosa esterase before absorption.Thus, have that the synthetic and combination of Chylomicron fat has the people of obstacle and the malabsorption that poultry can be caused fatsoluble vitamin in the patient of hepatic disease and poultry, the enterocyte, in a word, the pathological process of any infringement dietary fat digestion and absorption all can cause vitamin malabsorption and shortage.Human body and poultry under normal circumstances, the micron order fatsoluble vitamin has only 20～25% absorbance, and individual variation is very big.
In a word, the micron order vitamin is because its sensitivity to environmental factors, the particularity that in the organism physiology biochemical process, requires, thus cause its absorption rate low, biological value is not high, has influenced the application of vitamin in body-care and feed industry and aquaculture.
Nano material is familiar with by people just gradually as a kind of new state that material exists, and the scientific value of nanotechnology and nano material and application prospect are accepted by people gradually.The preparation of nano material and the theory and application research of correlated performance thereof among forming and developing, have been widely used in industries such as industry, agricultural, medical treatment and weaving as a new ambit at present.The utilization nanotechnology improves or changes water solublity, dispersibility and the absorbance of vitamin, improves between vitamin and the food processing, the compatibility between the feedstuff processing, is an effective way that addresses the above problem.
Summary of the invention
Purpose of the present invention just is to provide the vitamin microemulsion with nanoscale particle diameter of a kind of high dissolving, high absorption and high nutrition.
Another object of the present invention provides a kind of preparation method with nanoscale particle diameter vitamin microemulsion.
So-called nanometer vitamin, be meant and vitamin pulverized in the 100nm, atom, molecule or atomic group and the micel of direct manipulation vitamin by certain fine machining method, utilize compounded technology that it is rearranged, form novel form vitamin with nanoscale.Nanometer vitamin is the vitamin nanoparticle by zero dimension, the nanoscale discontinuous phase liquid that two and three dimensions vitamin nanostructure is formed, have and be different from micron granularity (the different physical characteristics of vitamin of 10um～25um), be its optics, calorifics, electricity, magnetics, mechanics, the chemistry aspect, physiological and biochemical procedure and trophism aspect, all have different significantly with supporting one's family of micron granularity, in specific surface area, surface activity, dissolubility, absorbance, trophism and have huge difference in the intravital physiological and biochemical procedure of machine and between the micron granularity vitamin has its unique dissolubility, absorbance, Physiology and biochemistry characteristics and to the high nutrition immune of body.
In the nanoscale vitamin microemulsion provided by the present invention, include the following component (parts by weight) of particle diameter less than 50nm:
50～150 parts of vitamin,
50～100 parts of oligomeric isomaltoses,
5～10 parts of emulsifying agents,
1～3 part of C
2～C
4Polyhydric alcohol,
5～10 parts of dextrin,
1～2 part of tetrasodium pyrophosphate and
1000 parts of deionized waters.
At least include a kind of fatsoluble vitamin in the described vitamin,
Described emulsifying agent is at least a in sucrose fatty acid ester, tween 80, the lecithin.
In order to change the structure and the state on vitamin nano_scale particle surface, the characteristic of nanometer vitamin is improved, in nanoscale vitamin microemulsion, can also add the inorganic salt sodium chloride of 50～200 weight portions, so that it is carried out finishing.
The concrete preparation method of nanoscale vitamin microemulsion is:
A. deionized water is heated to 70～90 ℃, the abundant stirring and dissolving of all the other raw materials that adds except that vitamin is made emulsion;
B. be cooled to 20～40 ℃, add vitamin, fully stirring and dissolving;
C. use high pressure homogenizer, under 70Mpa pressure, emulsion is carried out homogenizing first, and filter;
D. filtrate uses high pressure homogenizer to carry out homogenizing once more under 150Mpa pressure, and after filtering, obtains nano level vitamin microemulsion.
Consider that vitamin is the temperature-sensitive nutrient substance, only strict control temperature could guarantee not lose tiring of vitamin in preparation process.Therefore, the present invention has used chiller in homogenizing process, makes the temperature of emulsion remain on 20～30 ℃.
If the emulsion behind the adding vitamin re-uses the high pressure homogenizer homogenizing after using colloid mill to carry out thick emulsifying earlier, can reach better homogenizing effect.Emulsion behind the homogenizing and microemulsion all also need to use Merlon cylinder hole micro-filtrate membrane filtration.
Can include various vitamin in the nanoscale vitamin microemulsion of the present invention's preparation, both can be water soluble vitamins, also can be fatsoluble vitamin; Both can be single vitamin, also can be compound vitamin.Simultaneously, in order to increase the function of nanoscale vitamin microemulsion, we can also add some other nutritional labelings such as aminoacid, trace element etc., to form the application prescription of various difference in functionalitys.
Preparing the method that nanometer vitamin adopts is emulsion method, its mechanism is to utilize water, two kinds of mutual exclusive solvents of oil under the effect of surfactant, adopt high pressure homogenizer that liquid is pulverized the back and form a uniform emulsion, then by the finishing effect, avoid the reunion again between the fatsoluble vitamin oil droplet.One of key of this method is that each fatsoluble vitamin oil droplet is surrounded by a continuous water, promptly forms the microemulsion of oil-in-water (O/W) type, the characteristics that this heterogeneous liquid phase synthesizing method has narrow particle size distribution and controls easily.
Transparent isotropic thermodynamic stable system that the microemulsion of nanometer vitamin is made up of fatsoluble vitamin and/or water soluble vitamins, surfactant, stabilizing agent, oligosaccharide, aminoacid etc. and deionized water.In the microemulsion after pulverizing through high pressure homogenizer, small fatsoluble vitamin " oil droplet " is surrounded by the monolayer interface of compositions such as surfactant and stabilizing agent, forms the microemulsion granule, and its particle diameter is between 20～25nm.Small fatsoluble vitamin " oil droplet " yardstick is little and separated from one another, thereby does not constitute oil phase, is referred to as " accurate phase " usually (pseduophase), this special microenvironment, and we are referred to as " microreactor " (microreactor).
Nanometer vitamin microemulsion granule is ceaselessly being done Brownian movement, difference is supported one's family crude granule when running foul of each other, forming the surfactant at interface and the hydrocarbon chain of stabilizing agent can infiltrate mutually, meanwhile, the ion in a kind of fatsoluble vitamin " oil droplet " also can pass in " oil droplet " that the interface enters another kind of fatsoluble vitamin.This infiltration in " oil droplet " can be carried out between fatsoluble vitamin " oil droplet ", also can carry out between water soluble vitamins granule and fatsoluble vitamin " oil droplet ", in addition, also has ion to intersect by hydrocarbon key between the water soluble vitamins.The character of this mass exchange of microemulsion, make and formed " function synergic structure " between the different ions that function is helped mutually in the nanometer vitamin, this also is nanometer vitamin has higher trophism and better health care than the vitamin of micron granularity reason place.
Forming the essential condition of stablizing microemulsion is that suitable surfactant, i.e. emulsifying agent will be arranged.The emulsifying agent of selecting should improve the stability of microemulsion, former fatsoluble vitamin that can not mix and water are dissolved each other be uniform emulsion, the particle that guarantees discontinuous phase again less than 1um promptly within 100nm, to guarantee also that simultaneously microemulsion is a clear, any precipitation can not be arranged, that is to say, the fatsoluble vitamin " oil droplet " in the microemulsion is not reunited or polymerization when doing Brownian movement, again can not emulsifying strong excessively, influence the transparent of liquid phase.The content of the emulsifying effectiveness of emulsifying agent and emulsifying agent kind and fatsoluble vitamin has confidential relation.Though the emulsifying effectiveness of lipophilic emulsifier is better, behind the homogenizing, the microemulsion granule is assembled when doing Brownian movement easily mutually, makes the particle of discontinuous phase add greatly 500nm, and liquid phase is blue white, and the water-soluble back of microemulsion is a translucent liquid.The emulsifying effectiveness of hydrophilic emulsifier was both more satisfactory, and behind the homogenizing, the microemulsion granule is not assembled when doing Brownian movement, and the discontinuous phase particle can be controlled in the 50nm, can form high stability transparency liquid, the water-soluble back of microemulsion is the fluorescent transparency liquid.According to the HLB theory, when being complementary, the HLB of the HLB of emulsifying agent value and system value possesses best emulsifying effectiveness.Therefore, the emulsifying agent kind that the present invention finally determines is that HLB value is about 15 hydrophilic emulsifier, and as sucrose fatty acid ester and tween 80, it is 3～4 lipophilic emulsifier such as soybean lecithin that the while can also add a spot of HLB value.
Can free permeation in order to make between the different ions that function is helped mutually in the nanometer vitamin, form " function synergic structure ", also added micro-cosurfactant among the present invention, promoted the intergranular mass exchange of nanometer vitamin, the cosurfactant that the present invention adds is meant C
2～C
4Polyalcohols, as ethylene glycol, propylene glycol, glycerol etc.
The granular boundary intensity of nanoscale vitamin microemulsion all has very big influence to the forming process of nanoparticle and the quality of final product.If boundary strength is little, the interface is more open, and the mass exchange speed between the vitamin granule is excessive, and then the zonation of final products granular size can be very wide.The factor that influences boundary strength mainly contains the hydrocarbon chain length of the content and the alcohol of water content, interface alcohol.In microemulsion, water exists with associated water (or irreducible water boundwater) and two kinds of forms of Free water usually, and the former arranges closely the polar head of surfactant, and boundary strength increases, and the latter just in time in contrast.Along with the increase of water content in the system, associated water is saturated gradually, and the ratio of Free water increases, and the boundary strength of system is diminished.Low-carbon alcohols is as cosurfactant, be present between the surfactant molecule, usually the hydrocarbon chain of the hydrocarbon chain specific surface activating agent of low-carbon alcohols is short, therefore when the content of interface alcohol increases, space between the surfactant hydrocarbon chain can be strengthened, and when the vitamin granule collided mutually, infiltration easily also crossed one another between the interface, when as seen the content of interface alcohol increases, the boundary strength of system is descended.In the nanoscale vitamin microemulsion of the present invention, only containing the cosurfactant of trace, almost completely is the microemulsion that is formed by associated water, vitamin and surfactant, so the boundary strength of system is big, the particle size distribution of final products is narrower.
Consider that tiring of vitamin is very responsive for the reaction of pH value, the present invention has added a spot of tetrasodium pyrophosphate again as buffer agent in system, thereby guarantee that nanoscale vitamin microemulsion has a rational pH value scope, with the performance of tiring of abundant assurance vitamin.
So-called finishing, utilize method physics and chemistry to change the structure and the state of vitamin nano-particle surface exactly, thereby give the new function of vitamin nanoparticle and the characteristic of vitamin is improved, realize control the vitamin nano-particle surface.To the finishing of nanometer vitamin, expectation reaches following purpose: the dispersibility that improves and change the vitamin nanoparticle; Improve the surface activity of vitamin nanoparticle; Make the vitamin nanoparticle produce new physics, chemical characteristic and new trophic function; The non-covalent bond of sufficient amount is provided for the self assembly of vitamin nanostructured.
By van der waals force etc. emulsifying agent is adsorbed on the surface of vitamin nanoparticle, can prevents the reunion of fatsoluble vitamin nanoparticle.In the liquid crushing process of vitamin, add surfactant, exactly the nano grain surface of vitamin is modified.Surfactant has two kinds of basic physical chemical actions in nanometer vitamin, and the one, absorption and reduction surface activitv, the 2nd, micelle turns usefulness into.Contain the distinct functional group of two class character in the surfactant molecule, the one, polar group, possess hydrophilic property, another is a non-polar group, has lipophile.Fatsoluble vitamin nanoparticle in the nanometer vitamin is dispersed in the aqueous solution, the nonpolar lipophilic group of surfactant is adsorbed onto the surface of microgranule, and polarity hydrophilic group and water are molten, surface in the vitamin nanoparticle forms one deck molecular film, hinder contacting with each other between the granule, increased simultaneously the distance between the fatsoluble vitamin olesome again, make the olesome contact no longer tight, avoided the formation of bridge formation hydroxyl and real chemical bond, thereby realized finishing, the fatsoluble vitamin nano-particle can stably be dispersed in the aqueous solution the fatsoluble vitamin nanoparticle.
In nanometer vitamin, also added this nutritive sweetener of oligomeric isomaltose, the adding of oligomeric isomaltose, one side has been improved the trophic structure of vitamin, has also increased the viscosity of nanometer vitamin system on the other hand.According to the stocks formula, the increase of system viscosity can reduce ionic come-up of decentralized photo and subsidence velocity.Therefore, the adding of oligomeric isomaltose can improve the stability of nanometer vitamin system.
The present invention has also added inorganic salts such as sodium chloride in nanometer vitamin, these materials also can form nanoparticle and be distributed in the aqueous solution in the liquid crushing process.The nonpolar lipophilic group of surfactant is adsorbed onto microparticle surfaces, and hydrophilic group of polarity and water are molten, make the nanoparticle of inorganic salts such as sodium chloride in aqueous solution, can disperse well, significantly reduced the mutual collision opportunity of fatsoluble vitamin nanoparticle when doing Brownian movement, thereby reached finishing nanoparticle.
The process of surface treatment of vitamin nanoparticle is directly connected to the effect of finishing.Surface treatment can be carried out after liquid is pulverized, and also can carry out in liquid is pulverized, and experiment shows, it is best to carry out modification effect in liquid is pulverized.
The present invention uses high pressure homogenizer that the granule pulverizing of vitamin microemulsion is nanoscale.High pressure homogenizer is the powerful shearing force that is subjected to when utilizing liquid at high speed to flow through narrow gap, the impact force that produces during to the metal parts high speed impact, a series of resultant force effects that produced by high pressure such as hole explosive force because of the static pressure bust jumps and produces are ground into nano level stable microemulsion liquid with vitamin mixture.
All to produce lamination after the long-time placement of all food liquids.With fatsoluble vitamin of the present invention, water soluble vitamins, surfactant, stabilizing agent, oligosaccharide, aminoacid etc. with stir evenly after deionized water mixes, range estimation is more uniform emulsion in moment, yet after several minutes, the surface of emulsion can form the mixed layer of one deck fatsoluble vitamin, makes emulsion produce lamination.This is because when leaving standstill, oil droplet spheroidal particle come-up in the emulsion and the speed of sinking satisfy the stocks formula.If the density of olesome is bigger than the density of liquid medium, olesome just sinks, speed be on the occasion of; Otherwise olesome will float, and speed is negative value.The stocks formula shows that particulate sedimentation velocity and particle diameter d are proportional, and the reducing of particle diameter will significantly reduce sedimentation velocity, reduce the sedimentation velocity of olesome, can only reduce particulate diameter.But, when particulate diameter little of the branch period of the day from 11 p.m. to 1 a.m that approaches liquid medium, the stocks formula is just inapplicable, this moment is owing to formed bonding force between liquid medium molecule and molecule, make to separate and be difficult to take place, thereby form a homogeneous microemulsion, stable each other and do not disturb mutually between each component, greatly improved the stability of microemulsion.
The present invention utilizes the such coupling power principle of high pressure homogenizer just, fatsoluble vitamin, water soluble vitamins, surfactant, stabilizing agent, oligosaccharide, aminoacid, trace element etc. are mixed in proportion with deionized water, successively in the high pressure homogenizer of 70Mpa and 150Mpa, carry out liquid and pulverize.In this process, when the vitamin granule in the liquid is ground into nanoparticle under the combined effect of hole, turbulent flow and shearing force, other composition such as surfactant also is ground into nanoparticle simultaneously, form the unimolecule interface, rapidly with the fatsoluble vitamin embedding, make it no longer to produce polymerization, flow out with certain pressure at last, finish the pulverizing of nanometer vitamin liquid.
The pulverizing of vitamin liquid particles divides two steps to carry out among the present invention, the first step uses the high pressure homogenizer of 70Mpa pressure that the vitamin liquid particles is crushed to 800nm earlier, and the high pressure homogenizer of the second step reuse 150Mpa pressure continues liquid particles to be crushed to below the 50nm.What deserves to be mentioned is that adopt this technology, last finished product does not need to adopt disinfecting steps, because when 130Mpa pressure, the cell wall of noxious bacteria as long as note not causing secondary pollution when filtering, can omit disinfecting steps just by complete breaking cellular wall.
Temperature when using high pressure homogenizer to pulverize liquid particles is still very big for the influence of crushing effect, because the temperature height, saturated vapour pressure is just high, and the hole during pulverizing also forms easily.So, in order to improve crushing effect, under guaranteeing that liquid material can the prerequisite of degeneration, improve suitably that to pulverize temperature be effective.But because vitamin is to belong to the temperature-sensitive nutrient substance, so very crucial for temperature controlling.The experiment proved that material temperature remains on 20～30 ℃ in the crushing process, what can guarantee that vitamin tires does not lose.
For with in the nanoscale vitamin microemulsion in homogenizing process the antibacterial of breaking cellular wall death and remove greater than the particle of 50nm, it is the secondary filter isolation technics of material that the present invention has adopted with Merlon (PC) cylinder hole micro-filtration membrane.Through experiment confirm, adopt the micro-filtration membrane isolation technics that nanoscale vitamin microemulsion has been filtered following advantage:
1, the composition of vitamin is difficult for losing, and makes the nutrition in the nanometer vitamin keep good stable easily.
2, the micro-filtration membrane aperture is even, filtering accuracy height, good reliability.No medium comes off in the filter process, can not produce secondary pollution.
3, micro-filtration membrane belongs to absolute filter medium, and all protons greater than the micro-filtration membrane aperture, antibacterial all are trapped filtering, filters thoroughly.
4, there is not phase transition process in filtering process, but conserve energy.
We adopt laser scattering technology, and the DLS-700 type laser light scattering instrument that uses the big tomb electronics corporation of Japan to produce is measured the particle diameter of nanoscale vitamin microemulsion, and experimental temperature is 25 ± 4.5 ℃ during measurement, optical maser wavelength 632.8nm.The testing result granularity is 19.7～24.8nm, particle size distribution 100%.
With nanoscale vitamin microemulsion of the present invention water-soluble after, the liquid clear, do not have precipitation, deposit 1 year under the room temperature after, the vitamin rate of retaining is 99.6%.Nanoscale vitamin microemulsion is added in the mineral water by 1% amount, and add 0.05% sodium benzoate, after the capping 70 ℃ of following pasteurizations 20 minutes, deposited under the room temperature 1 year, the vitamin rate of retaining is to deposit under 97%, 5 ℃ 1 year, and the rate of retaining is 105%, and mineral water does not produce muddiness and precipitation, still clear because of vitamin enrichment.
The discontinuous phase liquid that nanometer vitamin is made up of water soluble vitamins nano-particle, fatsoluble vitamin nano-particle, aminoacid nano-particle, trace element nano-particle etc., these nanoparticles are referred to as " reaction tank ".Nanoparticle is ceaselessly done Brownian movement in liquid, different nanoparticles is when collision, owing to form the surfactant at interface, the hydrocarbon key of cosurfactant and stabilizing agent infiltrates mutually, ion in one " reaction tank " can enter into another " reaction tank ", makes these nanoparticles that multiple physical reactions and chemical reaction take place.This special microenvironment is referred to as " microreactor ", is the perfect medium of various health substances and immune material generation catalytic reaction, complexation reaction and complex reaction.By studies confirm that, the nanoscale compound vitamin that harsh output is come, its abnormal smells from the patient and mouthfeel are general, after placement fortnight, strong the assailing the nostrils of vitamin flavor that will become, fragrance is good to eat, to the anti-stress effect and the also raising greatly of supply effect of body, so the nanoscale compound vitamin has the derivatization process of dynamic " function synergic structure ".
In fact the derivatization process of " the function synergic structure " of nanoscale compound vitamin is exactly the forming process of the self-assembly system or the molecule self-assembly system of vitamin nanostructured.
The self-assembly system of nanostructured is meant that the synergism as hydrogen bond and Fan Deer gas key links together atom, ion and molecule, is built into the style (pattern) of a nanostructured or nanostructured by weak and non-covalent bond less direction.The key of self assembling process is not the simple superposition of a large amount of atoms, ion and intermolecular weak force, but a kind of synergism of system of integral body.The self-assembly system of nanostructured be formed with two important conditions: the one, have the non-covalent bond of sufficient amount or hydrogen bond to exist, this is because non-covalent bonds very weak (0.15kcal/mol) such as hydrogen bond and Fan Deer gas keys, have only the weak bond of q.s to exist, could pass through synergism, be built into stabilized nano structural system; The 2nd, the self-assembly system energy is lower, otherwise, be difficult to form stable self-assembly system.
The molecule self-assembly system be molecule and molecule under equilibrium condition, rely on intermolecular non-covalent bonding force, the spontaneous process that is combined into stable molecule aggregation body (aggregates).Build the molecule self-assembly system and will be divided into 3 levels: the first, orderly covalent bond at first is combined into baroque, complete intermediate section daughter; The second, by the synergism of intermediate section daughter, form constitutionally stable big molecule aggregate by weak hydrogen bond, Fan Deer gas key and other non-covalent bond; The 3rd, make construction unit by one or several molecule aggregate, repeatedly repeat self assembly, be arranged in the nanostructured system.This that is to say, nanostructure that the nanoscale compound vitamin is made up of many vitamin and vitamin, vitamin and other nutrient substance and big molecule aggregate are formed.For example; " the function synergic structure " that vitamin E and vitamin C form, its anti-oxidation function can be strengthened greatly, this is after having shortened vitamin E and radical reaction, obtains due to the regenerated path by vitamin C, because these processes can directly be carried out on " function synergic structure ".Again for example, vitamin A and vitamin B
6" the function synergic structure " that forms, its function that prevents seborrheic alopecia is strengthened greatly.Also has vitamin B
2, vitamin B
6" function synergic structure " with zinc is formed can prevent the dandruff better.
Just because of so characteristic is arranged, so the health care of nanometer vitamin and the formation of immunologic function, it is not the simple superposition of the health care of several vitamin and immunologic function and compound, but all have between the health care and the material of immunologic function by nanostructured and intermolecular self assembly form a kind of novel fresh and alive, synergism is stronger, the compound vitamin that " function synergic structure " existence of unique nourishing healthy immunologic function is arranged, a kind of like this compound vitamin of configuration state, nature may exist, and also may be the still non-existent new nutrient substance of nature.It is comparatively outstanding that the derivatization process of this " function synergic structure " shows in the compound vitamin product, and what then show in the product of individual event vitamin is not obvious.
Skin effect is meant that the ratio of nano-particle surface atomic number and total atom number diminishes with particle diameter and sharply increases the caused qualitative change in back.We know, square being directly proportional of the surface area of spheroidal particle and radius, and cube being directly proportional of volume and radius is so its specific surface area (surface area/volume) is inversely proportional to radius.Along with reducing of nanoparticle particle diameter, surface area sharply increases, and surface atom percent also increases sharply.The particle diameter of nanometer vitamin is 20～25nm, and specific surface area is 70nm
2/ g, such specific surface area makes the atomic number that is in the surface more and more, and simultaneously, surface energy also increases sharply.Because nanometer vitamin surface atom number increases, specific surface area increases, and causes the Atomic coordinate deficiency, produce a large amount of dangling bonds and unsaturated bond, surface energy increases in addition, causes these surfaces to have high activity, extremely unstable, be easy to combine with other atom.The activity of this surface atom not only causes the variation of nanoparticle atom transport and configuration, also can cause the variation of surface electronic structure picture and electron spectrum simultaneously.
Because nanometer vitamin has skin effect, so also possessed following physical characteristic:
1, diffusibility is good.Diffusing phenomenon are meant when having concentration difference, the material transport phenomena that causes owing to the microgranule warm-up movement.Because skin effect makes nanometer vitamin have higher chemism, the diffusion coefficient of vitamin increases, a large amount of interfaces provides highdensity short distance fast the evolving path for the atom diffusion, even make nanometer vitamin in cold water, also can moment instant, be uniformly dispersed.The particle diameter of adding the nanometer vitamin microgranule approaches hydrone, and is extremely strong with the bonding force of water, causes to have produced high dissolving diffusibility, can unrestrictedly close with water-soluble, can not produce the oil bloom of fatsoluble vitamin on the surface of water layer.
2, dialysis performance is good.After nanometer vitamin is water-soluble, can not change the transparency of water, make vitamin have very good dialysis performance.Nanometer vitamin is coated in skin surface, just can enters blood by transdermal in 3～5 minutes, this has crucial meaning for the forced for vitamins in the cosmetics.During culinary art vitamin food, this specific character of nanometer vitamin makes its easier cooking food interior tissue that enters, nanometer vitamin Surface binding energy height in addition, can make the distribution of fatsoluble vitamin in food more even, mouthfeel is purer, mellow, this also has crucial meaning for the reinforcement of vitamin in the food industry.This specific character of nanometer vitamin makes when being added on it in the drinking-water of poultry, all poultry of drinking-water can both drink the vitamin of equivalent, and in drinking trough, pond or barrel cage, do not precipitate, also can not stop up the drinking-water nipple, this has crucial Economics and Management meaning for aquaculture.
3, absorbing property height.Fatsoluble vitamin is hydrophilic in the nanometer vitamin, is in the colloid dispersity again, thereby is a kind of thermodynamic stable system.The more important thing is that the fatsoluble vitamin of 20～25nm has improved fatsoluble vitamin at the intravital pharmacokinetic properties of people, mainly shows three aspects: the first, the nanoscale fatsoluble vitamin can make the absorption of fatsoluble vitamin obviously increase.Nanoscale fatsoluble vitamin liquid can make fatsoluble vitamin discharge rapidly in gastrointestinal, and excellent contact is arranged with the gastrointestinal tract epithelial layer, by matter between gastrointestinal tract epithelial cell, pass intestinal and enter blood circulation, the result absorbs fatsoluble vitamin easily, and bioavailability obviously improves.The average bioavailability of common fatsoluble vitamin is about 30%, and the average bioavailability of nanoscale fatsoluble vitamin can reach 90%.The second, the absorption of nanoscale fatsoluble vitamin is rapider, and the average out to peak time in advance.The 3rd, the nanoscale fatsoluble vitamin can make interior medicine dynamics stability improve, and the individual variation of replenishing between the fatsoluble vitamin person diminishes.The absorption of nanoscale fatsoluble vitamin is subjected to the influence (as the influence of fat content in bile, secretion, feed and the food) of gastrointestinal tract physiological status smaller, can just not arrive the surface of enterocyte by the bile dissolving, by non-carrier mediated passive diffusion, enter the intestinal mucosa cell.And suffer from the patient or the animal of hepatic disease and fat malabsorption, and can not influence the absorption of fatsoluble vitamin, make the absorption of nanoscale fatsoluble vitamin reach 100%.This has crucial meaning in pharmaceuticals industry, also provides example for nanotechnology in the application of nourishing healthy and field of medicaments simultaneously.
The absorption of water soluble vitamins and aminoacid etc., be to enter small intestinal mucosa by facilitation diffusion, passive transport and the active transport by sodium pump, and the active transport of sodium pump also has only the concentration Gao Shicai when aminoacid and water soluble vitamins to work, thus, the granule size of aminoacid and water soluble vitamins, dissolution degree and with the effective contact area of gastrointestinal, be the key that aminoacid and water soluble vitamins absorb.Nanoscale water soluble vitamins and amino acid whose microgranule have strengthened effective contact area of water soluble vitamins and aminoacid and gastrointestinal tract cell with its unique skin effect, interfacial effect and small-size effect, thereby have improved absorbance and bioavailability.This has crucial physiological significance in time replenishing of human body and animal limiting amino acid and water soluble vitamins.
Nanometer vitamin is compared with the vitamin of micron order granularity, because its skin effect, greatly improve dissolubility, dispersibility and the dialysis performance of vitamin in water, changed vitamin physiological and biochemical procedure in vivo, and eliminated the difference that absorbs between individuality.
(fatsoluble vitamin in the powdery compound vitamin of 150um～500um) is that the reuse gelatin wraps quilt after adopting adsorbents adsorb to general micron order.And the fatsoluble vitamin in the emulsifying compound vitamin is after adopting surfactant, glucose and dextrin emulsifying, to obtain through homogenizing, oven dry, particle.The experiment proved that the fatsoluble vitamin absorbance in the powdery compound vitamin is 20～25%, bioavailability is 30%; Fatsoluble vitamin absorbance in the emulsifying compound vitamin is 40～45%, and bioavailability is 55%; And the absorbance of the fatsoluble vitamin in the nanoscale compound vitamin can reach 100%, and bioavailability reaches more than 90%.
Studies show that applying nano level vitamin can reduce dosage 20～30%, integrated cost reduces about 30%.
Nanoscale vitamin microemulsion of the present invention is owing to adopted advanced interface technology, make full use of surface tension different between vitamin, with vitamin moment superfine powder be broken into nano-scale particle, and on molecular level moment carry out the profound embedding of biomembrane, make different pH values, different thermal sensitivities, vitamin and other nutrient substance of different light sensitivities and different oxidoreduction degree are co-located in the liquid of same discontinuous phase, stable each other and do not disturb mutually, solved well because the envirment factor instability, the problem that makes vitamin tire and reduce, greatly improved vitamins stability, shelf life can reach more than 2 years.
In the processing process of nanometer vitamin, adopt cryogenic high pressure technology that the cell wall of noxious bacteria is broken fully, make its inactivation, pass through micro-filtrate membrane filtration technology again with its elimination.Thus, nanometer vitamin is real safety asepsis.
Nanoscale vitamin microemulsion of the present invention can be widely used in different industries.
Feedstuff industry
Nanometer vitamin, aminoacid and other nutritional labeling are treated as the nanoscale color grains, learn principle according to nano nutrient and be re-dubbed premix material, but both spice, also can drink water, the bioavailability of body is high, and can judge the mixing homogeneity of feedstuff by the distribution of color grains in feedstuff.
Nanoscale liquid vitamin can be widely used in the raising of young stock poult and the usefulness of anti-stress, also can be made into the back spray coating liquor with other heat-sensitive materials such as microbial ecological agent etc., is applied in the production of expanded pellet diet.
Add modified starch in the homogenizing process of nanometer vitamin, by high-pressure spray-drying, can also obtain the nanometer vitamin powder, the particle diameter of nanometer vitamin powder ratio nano level vitamin microemulsion is wanted big 10～15nm.
Cosmetic industry
Nanometer vitamin can add in the cosmetics and goes.As fruit cosmetic is optically transparent, and effect is better when adding product of the present invention.These cosmetics comprise any Related product that is used for skin, hair, but will note adding nanometer vitamin at the appropriate time of production process, to keep tiring and fully mixing with cosmetics of vitamin.
Pharmaceuticals industry
Nanometer vitamin can be widely used in pharmaceuticals industry, as makes injection, transfusion liquid, tablet etc., is used for the treatment of the auxiliary treatment of avitaminosis and relevant disease.
Beverage industry
Nanometer vitamin can be widely used in beverage industry.Here said beverage comprises soda pop, mineral water, various fruit beverage, chocolate, coffee, milk, beverage made of fruits or vegetables, sports drink and bottled water etc.Nanometer vitamin is best as the additive effect of sports drink, because the skin effect of nanometer vitamin can be replenished fast because of excited and the required nutritional labeling of vigorous exercise.
Health products trade
Nanometer vitamin can be widely used in health products trade.According to human body every day required various vitamin, essential amino acids, trace element, electrolytic ion etc. form prescription, strengthen again and advance bifidus factor and immune factor, handle by nanorize then and make oral liquid, no matter from the composition of prescription, still from absorbance, bioavailability and the potency ratio aspect of nutrient, its incomparable advantage is arranged all.
The specific embodiment
Embodiment 1
1000 ml deionized water are heated to 80 ℃, add 5 gram sucrose fatty acid ester, 5 gram dextrin, 1 gram propylene glycol, 1 gram tetrasodium pyrophosphate and 50 gram oligomeric isomaltoses, stirring and dissolving; Be cooled to 30 ℃, slowly add 55 gram Vitamin E acetate; After carrying out emulsifying by colloid mill, thick emulsion enters homogenizing in the 70Mpa high pressure homogenizer, and use micro-filtrate membrane filtration, filtrate enters the high pressure homogenizer of 150Mpa pressure, homogenizing circulates, after reaching about 25nm to the granule of emulsion, use micro-filtrate membrane filtration, obtain about 5% vitamin E transparent micro emulsion.In homogenizing process, be maintained at about about 30 ℃ with the temperature of cooling system with emulsion.
This product is mainly used in cosmetic industry and beverage industry.
Embodiment 2
1000 ml deionized water are heated to 75 ℃, add 5 gram sucrose fatty acid ester, 5 gram dextrin, 1 gram propylene glycol, 1 gram tetrasodium pyrophosphate and 50 gram oligomeric isomaltoses, stirring and dissolving; Be cooled to 30 ℃, add 55 gram beta-carotene; After emulsion is carried out emulsifying by colloid mill, thick emulsion enters homogenizing in the 70Mpa high pressure homogenizer, and use micro-filtrate membrane filtration, filtrate enters the high pressure homogenizer of 150Mpa pressure, homogenizing circulates, after reaching about 25nm to the granule of emulsion, use micro-filtrate membrane filtration, can obtain about 5% beta-carotene transparent micro emulsion.In homogenizing process, be maintained at about about 30 ℃ with the temperature of cooling system with emulsion.
This product is mainly used in beverage industry.
Embodiment 3
1000 ml deionized water are heated to 85 ℃, add 5 gram sucrose fatty acid ester then, 1 gram lecithin, 8 gram dextrin, 2 gram glycerol, 1 gram tetrasodium pyrophosphate, 60 gram oligomeric isomaltoses stir and are cooled to 30 ℃, add 55 gram vitamin A acetate, 20 gram vitamin D
3, after emulsion was carried out emulsifying by colloid mill, thick emulsion entered homogenizing in the 70Mpa high pressure homogenizer, and the use micro-filtrate membrane filtration, filtrate enters the high pressure homogenizer of 150Mpa pressure, and homogenizing circulates, after reaching about 25nm to the granule of emulsion, use micro-filtrate membrane filtration, can obtain vitamin AD
3Transparent micro emulsion.In homogenizing process, be maintained at about about 30 ℃ with the temperature of cooling system with emulsion.
This product is mainly used in beverage industry, feedstuff industry.
Embodiment 4
1000 ml deionized water are heated to 85 ℃, add 5 gram sucrose fatty acid ester then, 1 gram lecithin, 8 gram dextrin, 1 gram propylene glycol, 1 gram tetrasodium pyrophosphate, 60 gram oligomeric isomaltoses stir and are cooled to 30 ℃, add 55 gram vitamin A acetate, 20 gram vitamin D
340 gram vitamin Cs, after emulsion is carried out emulsifying by colloid mill, thick emulsion enters homogenizing in the 70Mpa high pressure homogenizer, and uses micro-filtrate membrane filtration, and filtrate enters the high pressure homogenizer of 150Mpa pressure, homogenizing circulates, after reaching about 25nm to the granule of emulsion, use micro-filtrate membrane filtration, can obtain the compound vitamin transparent micro emulsion.In homogenizing process, be maintained at about about 30 ℃ with the temperature of cooling system with emulsion.
This product is mainly used in beverage industry, feedstuff industry.
Embodiment 5
1000 ml deionized water are heated to 80 ℃, add 5 gram sucrose fatty acid ester then, 1 gram lecithin, 8 gram dextrin, 1 gram propylene glycol, 1 gram tetrasodium pyrophosphate, 60 gram oligomeric isomaltoses stir and are cooled to 30 ℃, add 55 gram vitamin A acetate, 20 gram vitamin Es, 40 gram vitamin Cs; After carrying out emulsifying by colloid mill, thick emulsion enters homogenizing in the 70Mpa high pressure homogenizer, and use micro-filtrate membrane filtration, filtrate enters the high pressure homogenizer of 150Mpa pressure, homogenizing circulates, after reaching about 25nm to the granule of emulsion, use micro-filtrate membrane filtration, can obtain the compound vitamin transparent micro emulsion.In homogenizing process, be maintained at about about 30 ℃ with the temperature of cooling system with emulsion.
This product is mainly used in beverage industry.
Embodiment 6
1000 ml deionized water are heated to 80 ℃, add 5 gram sucrose fatty acid ester then, 2 gram tween 80s, 8 gram dextrin, 1 gram propylene glycol, 2 gram tetrasodium pyrophosphates, 50 gram oligomeric isomaltoses, 50 gram sodium chloride, stirring and dissolving; Be cooled to 30 ℃, slowly add 10 gram Vitamin E acetate, 25 gram vitamin A acetate, 10 gram vitamin D
3Oil, 7 gram vitamin Ks
3Oil, 1 gram vitamin B
1, 3 gram vitamin Bs
2, 1 gram vitamin B
6, 1 gram vitamin B
12(1%), 10 gram nicotinic acid, 25 gram pantothenic acid, 0.3 gram folic acid, 20 gram vitamin Cs, 15 gram choline chlorides; After carrying out emulsifying by colloid mill, thick emulsion enters homogenizing in the 70Mpa high pressure homogenizer, and use micro-filtrate membrane filtration, filtrate enters the high pressure homogenizer of 150Mpa pressure, homogenizing circulates, after reaching about 25nm to the granule of emulsion, use micro-filtrate membrane filtration, can obtain the compound vitamin transparent micro emulsion.In homogenizing process, be maintained at about about 30 ℃ with the temperature of cooling system with emulsion.
This product is mainly used in feedstuff industry.
Embodiment 7
1000 ml deionized water are heated to 85 ℃, add 5 gram sucrose fatty acid ester then, 5 gram dextrin, 2 gram glycerol, 1 gram tetrasodium pyrophosphate, 100 gram oligomeric isomaltoses, 50 gram sodium chloride, stirring and dissolving; Be cooled to 30 ℃, slowly add 10 gram Vitamin E acetate, 25 gram vitamin A acetate, 10 gram vitamin D
3Oil, 7 gram vitamin Ks
3Oil, 1 gram vitamin B
1, 3 gram vitamin Bs
2, 1 gram vitamin B
6, 1 gram vitamin B
12(1%), 10 gram nicotinic acid, 25 gram pantothenic acid, 0.3 gram folic acid, 20 gram vitamin Cs, 15 gram choline chlorides add 40 gram methionine again, 40 gram lysines; After carrying out emulsifying by colloid mill, thick emulsion enters homogenizing in the 70Mpa high pressure homogenizer, and use micro-filtrate membrane filtration, filtrate enters the high pressure homogenizer of 150Mpa pressure, homogenizing circulates, after reaching about 25nm to the granule of emulsion, use micro-filtrate membrane filtration, can obtain the compound vitamin transparent micro emulsion.In homogenizing process, be maintained at about about 30 ℃ with the temperature of cooling system with emulsion.
This product is mainly used in feedstuff industry.
Embodiment 8
1000 ml deionized water are heated to 85 ℃, add 5 gram sucrose fatty acid ester then, 3 gram tween 80s, 5 gram dextrin, 2 gram propylene glycol, 1 gram tetrasodium pyrophosphate, 100 gram oligomeric isomaltoses, 50 gram sodium chloride, stirring and dissolving; Be cooled to 30 ℃, slowly add 10 gram Vitamin E acetate, 25 gram vitamin A acetate, 10 gram vitamin D
3Oil, 7 gram vitamin Ks
3Oil, 1 gram vitamin B
1, 3 gram vitamin Bs
2, 1 gram vitamin B
6, 1 gram vitamin B
12(1%), 10 gram nicotinic acid, 25 gram pantothenic acid, 0.3 gram folic acid, 20 gram vitamin Cs, 15 gram choline chlorides add 40 gram methionine again, 40 gram lysines, 40 ml milk colostrum extracts; After carrying out emulsifying by colloid mill, thick emulsion enters homogenizing in the 70Mpa high pressure homogenizer, and use micro-filtrate membrane filtration, filtrate enters the high pressure homogenizer of 150Mpa pressure, homogenizing circulates, after reaching about 25nm to the granule of emulsion, use micro-filtrate membrane filtration, can obtain the compound vitamin transparent micro emulsion.In homogenizing process, be maintained at about about 30 ℃ with the temperature of cooling system with emulsion.
This product is mainly used in feedstuff industry.
Embodiment 9
1000 ml deionized water are heated to 85 ℃, add 5 gram sucrose fatty acid ester then, 3 gram tween 80s, 5 gram dextrin, 2 gram glycerol, 2 gram tetrasodium pyrophosphates, 100 gram oligomeric isomaltoses, 50 gram sodium chloride, stirring and dissolving; Be cooled to 35 ℃, slowly add 10 gram Vitamin E acetate, 25 gram vitamin A acetate, 10 gram vitamin D
3Oil, 7 gram vitamin Ks
3Oil, 1 gram vitamin B
1, 3 gram vitamin Bs
2, 1 gram vitamin B
6, 1 gram vitamin B
12(1%), 10 gram nicotinic acid, 25 gram pantothenic acid, 0.3 gram folic acid, 20 gram vitamin Cs, 15 gram choline chlorides add 40 gram methionine again, 40 gram lysines, 40 ml milk colostrum extracts, 40 gram sorbitols; 40 gram ammonium chloride, 20 gram sodium sulfate, 20 gram magnesium sulfate, 30 gram potassium chloride, 30 gram zinc sulfate, carry out emulsifying by colloid mill after, thick emulsion enters homogenizing in the 70Mpa high pressure homogenizer, and use micro-filtrate membrane filtration, filtrate enters the high pressure homogenizer of 150Mpa pressure, the homogenizing that circulates, reach about 25nm to the granule of emulsion after, use micro-filtrate membrane filtration, can obtain the compound vitamin transparent micro emulsion.In homogenizing process, be maintained at about about 30 ℃ with the temperature of cooling system with emulsion.
This product is mainly used in feedstuff industry.
Embodiment 10
1000 ml deionized water are heated to 85 ℃, add 8 gram sucrose fatty acid ester then, 10 gram dextrin, 2 gram propylene glycol, 2 gram tetrasodium pyrophosphates, 100 gram oligomeric isomaltoses and 50 gram sodium chloride, stirring and dissolving; Be cooled to 35 ℃, slowly add 8 gram Vitamin E acetate, 20 gram vitamin A acetate, 10 gram vitamin D
3Oil, 7 gram vitamin Ks
3Oil, 1 gram vitamin B
1, 3 gram vitamin Bs
2, 1 gram vitamin B
6, 1 gram vitamin B
12(1%), 10 gram nicotinic acid, 25 gram pantothenic acid, 0.3 gram folic acid, 20 gram vitamin Cs, 15 gram choline chlorides add 40 gram methionine again, 40 gram lysines, 60 ml milk colostrum extracts, 40 gram sorbitols; After carrying out emulsifying by colloid mill, thick emulsion enters homogenizing in the 70Mpa high pressure homogenizer, and use micro-filtrate membrane filtration, filtrate enters the high pressure homogenizer of 150Mpa pressure, homogenizing circulates, after reaching about 25nm to the granule of emulsion, use micro-filtrate membrane filtration, can obtain the compound vitamin transparent micro emulsion.In homogenizing process, be maintained at about about 30 ℃ with the temperature of cooling system with emulsion.
This product is mainly used in health products trade.
Claims (6)
1, nanoscale vitamin microemulsion is characterized in that adding in 1000 parts of deionized waters:
50～150 parts of vitamin
50～100 parts of oligomeric isomaltoses
5～10 parts of emulsifying agents
1～3 part of C
2～C
4Polyhydric alcohol
5～10 parts of dextrin
1～2 part of tetrasodium pyrophosphate
Form the homogeneous phase microemulsion of liquid particle diameter less than 50nm,
At least include a kind of fatsoluble vitamin in the described vitamin,
Described emulsifying agent is made of at least a in sucrose fatty acid ester, tween 80, the lecithin,
Above umber is parts by weight.
2, nanoscale vitamin microemulsion according to claim 1 is characterized in that also being added with the sodium chloride of 50～200 weight portions in microemulsion.
3, the preparation method of the described nanoscale vitamin microemulsion of claim 1 is characterized in that may further comprise the steps:
A. deionized water is heated to 70～90 ℃, adds the abundant stirring and dissolving of all the other raw materials except that vitamin;
B. emulsion is cooled to 20～40 ℃, adds vitamin, fully stirring and dissolving;
C. use high pressure homogenizer under 70Mpa pressure to the emulsion homogenizing, and filter;
D. filtrate is used high pressure homogenizer homogenizing once more under 150Mpa pressure, and after filtering, obtains nano level vitamin microemulsion.
4, the preparation method of nanoscale vitamin microemulsion according to claim 3 is characterized in that in homogenizing process, makes the temperature of emulsion remain on 20～30 ℃ by cooling.
5, the preparation method of nanoscale vitamin microemulsion according to claim 3, the emulsion that it is characterized in that adding behind the vitamin uses colloid mill to carry out thick emulsifying earlier, re-uses the high pressure homogenizer homogenizing.
6, the preparation method of nanoscale vitamin microemulsion according to claim 3 is characterized in that emulsion and the microemulsion behind the homogenizing uses Merlon cylinder hole micro-filtrate membrane filtration.
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| CN101028240B (en) * | 2007-03-29 | 2010-06-09 | 中国科学院上海药物研究所 | Micro-emulsion/submicro-emulsion in-situ gel preparation for eyes and its making method |
| CN101297680B (en) * | 2008-04-25 | 2010-11-03 | 天津生机集团股份有限公司 | Composite vitamin emulsion and preparation thereof |
| CN101766261B (en) * | 2010-01-11 | 2011-09-28 | 广东大华农动物保健品股份有限公司 | Nano nutrient microemulsion and preparation method thereof |
| CN101828645A (en) * | 2010-05-19 | 2010-09-15 | 格特生物制药（天津）有限公司 | Nano-scale multifunctional liquid premixed feed and preparation method thereof |
| CN101978851B (en) * | 2010-08-17 | 2013-01-30 | 格特生物制药（天津）有限公司 | Three-in-one multifunctional premixed liquid feed and preparation method thereof |
| CN102626190A (en) * | 2012-04-28 | 2012-08-08 | 陕西金冠牧业有限公司 | Nanometer vitamin complex for breeding laying hens, preparing method therefor and usage thereof |
| CN104434968A (en) * | 2013-09-22 | 2015-03-25 | 天津市肾友达医疗设备技术开发有限公司 | Dialysis concentrate supplementing manganese element |
| CN104434969A (en) * | 2013-09-22 | 2015-03-25 | 天津市肾友达医疗设备技术开发有限公司 | Dialysis concentrate for supplementing copper elements |
| CN104434959A (en) * | 2013-09-22 | 2015-03-25 | 天津市肾友达医疗设备技术开发有限公司 | Dialysis concentrate containing vitamins |
| CN104434962A (en) * | 2013-09-22 | 2015-03-25 | 天津市肾友达医疗设备技术开发有限公司 | Dialysis concentrate for supplementing molybdenum elements |
| CN104304797B (en) * | 2014-10-30 | 2017-10-13 | 四川中农致远生物科技有限公司 | A kind of manufacturing process of livestock and poultry B B-complex nano-emulsion |
| CN104397702A (en) * | 2014-12-11 | 2015-03-11 | 福州乾正药业有限公司 | Lecithin-containing aqueous emulsifying agent as well as preparation method and application thereof |
| CN104522323B (en) * | 2014-12-19 | 2017-06-20 | 杨凌益佳泰生物科技有限公司 | A kind of bicontinuous nano-emulsion multidimensional and preparation method thereof |
| CN112088990A (en) * | 2019-06-17 | 2020-12-18 | 中国农业大学 | Nano-composite vitamin oral liquid and preparation method and application thereof |
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