CN1233261C - Fodder special for livestock and manufacturing method thereof - Google Patents

Fodder special for livestock and manufacturing method thereof Download PDF

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Publication number
CN1233261C
CN1233261C CNB2004100061919A CN200410006191A CN1233261C CN 1233261 C CN1233261 C CN 1233261C CN B2004100061919 A CNB2004100061919 A CN B2004100061919A CN 200410006191 A CN200410006191 A CN 200410006191A CN 1233261 C CN1233261 C CN 1233261C
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blood
lactic acid
feed
dry
acid bacteria
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CN1557183A (en
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汐見修一
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Guangzhou Zephyr Trading Co Ltd
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Abstract

The cattle feed is produced by the following process: admixing lactobacillus dried matter where a viable cell rate of the Lactobacillus fermentum is 1.1*10<SP>9</SP>to 1.2*10<SP>10</SP>cfu/g with blood of the livestock; fermenting the mixture; and making the fermented mixture absorb separation liquid obtained through solid-liquid separation.

Description

Domestic animal is used the feed manufacture method
Technical field
The invention belongs to domestic animal and use the feed manufacture method.
Technical background
Add antibiotics material (medicine) domestic animal in feed recently and bring negative effect to human body, the cattle breeding environmental pollution, traditional feed addictive can't keep the good health status of domestic animal, can not remove the feeding environment of stench, and meat can not be delicious more simultaneously.
Summary of the invention
Domestic animal of the present invention is to be 1.1 * 10 with density with the manufacture method of feed 9-1.2 * 10 10The lactobacillus fermenti of cfu/g is made the dry composition of lactic acid bacteria with the powder of average particulate diameter 1-9 μ m, after the mixing animal blood ferments, zymotic fluid is carried out Separation of Solid and Liquid and the dry thing of adsorbing separation liquid, uses feed thereby obtain dry domestic animal.
Utilize the present invention, domestic animal can be kept good health status, grows smoothly, and the body weight increase significantly improves.And delicious meat, best in quality.
This feed can keep off infection and other diseases, and can reduce the use of excessive medicines such as antibiotic input, improves meat.
Though utilized blood be not allow its corrupt but obtain can long preservation feed.
And the manufacture method of this feed is simple and easy, and feeding quality is stable.
Description of drawings
Fig. 1 is the process chart of lactic acid bacteria dry ingredient
Specific implementation method
The domestic animal that is proposed among the present invention is to be 1.1 * 10 with density with feed 9-1.2 * 10 10The lactobacillus fermenti of cfu/g is made the dry composition of lactic acid bacteria (the dry composition of high concentration lactic acid bacteria) with the powder of average particulate diameter 1-9 μ m, after the mixing animal blood ferments, by obtaining behind Separation of Solid and Liquid and the adsorbing separation liquid.
And this domestic animal is to be 1.1 * 10 with density with the manufacture method of feed 9-1.2 * 10 10The lactobacillus fermenti of cfu/g is made the dry composition of lactic acid bacteria (the dry composition of high concentration lactic acid bacteria) with the powder of average particulate diameter 1-9 μ m, has and mixes the operation that animal blood ferments.
Further describe, lactobacillus fermenti quantity density is 1.1 * 10 9-1.2 * 10 10The lactobacillus fermenti of cfu/g is made the dry composition of lactic acid bacteria (the dry composition of high concentration lactic acid bacteria) with the powder of average particulate diameter 1-9 μ m, after the mixing animal blood ferments (fermentation procedure), by Separation of Solid and Liquid (separation circuit) and adsorbing separation liquid, dry attachment (adsorption dry operation) thus obtaining domestic animal uses feed.
Before fermentation procedure, suitable blood with domestic animals such as ox, pigs carries out preliminary treatment by the initial stage operation.Because in the initial stage operation, heating can destroy the erythrocytic cell membrane that contains in the blood, thereby therefore the stripping hemoglobin at first carries out heat treated (hyperthermic treatment) to blood.
The heating-up temperature of blood is advisable with 50 ℃-75 ℃, and the lower limit special provision is to be because this temperature is fit to eliminate pathogen and corrupt mushroom more than 50 ℃.
If reaching lower limit, heating-up temperature can't not destroy red blood cell, and if surpass the upper limit then can cause rotten, the variation of blood.
Be to be advisable in 1-5 hour heat time heating time, and heating means can adopt methods known today such as being blown into steam not limit.
Thus, because can destroy blood platelet and leucocyte, cause cytoplasm to flow out the extracellular, so the fermentation process of blood is more easy.And the heat treated of blood can not only make the hemoglobin in the red blood cell separate out, and the effect of eliminating corrupt mushroom in the blood is arranged, and can cause lactic fermentation thereafter.
In the fermentation procedure, blood is cooled to 20 ℃-40 ℃ after finishing the initial stage operation, adding with density is 1.1 * 10 9-1.2 * 10 10The dry composition of lactic acid bacteria that the lactobacillus fermenti of cfu/g is made with the powder of average particulate diameter 1-9 μ m carries out the lactic fermentation operation.
Be advisable with weight ratio 0.03%-0.7% between high concentration lactic acid bacteria dry ingredient and the blood.As then fermenting insufficient less than lower limit.
And if surpassed the upper limit, then lactic acid bacteria could be too much, and be helpless to improve effect, the effect of fermentation.
Lactic acid fermented temperature is advisable with 30 ℃-43 ℃, the fermentation fate was limited in 48 hours-72 hours, was standard stuck fermentation engineering with concentration of lactic acid and pH value, if lactic fermentation is insufficient, then blood protein solidifies insufficiently, cause certain difficulty can for separation circuit thereafter.
Air supply that should be an amount of in the fermentation procedure is handled by fermentation, and labile organic matter is stablized the biochemical characteristic of blood in the decomposition blood.Thereby obtain the high protein of 55%-60%.
Contain sugar, fat, protein, nucleic acid, vitamin and mineral matter and other components in the blood, and the red blood cell red eggs contain a large amount of hemoglobinases in white, the words of throwing something and feeding as feed stripped can be grown up to domestic animal and be played very large effect.
But all the time, the protein that hemoglobinase in the blood is easy to be produced by corrupt thalline decomposes yeast and decomposes, and air supply on one side, thereby carry out the disappearance that is decomposed of hemoglobinase in organic feed stripped that the air fermentation makes, do not have the function of hemoglobinase.The way that the present invention proposes can be made organic feed stripped under the situation that does not lose the hemoglobinase function that contains in the blood.
That is to say, utilize above-mentioned lactic acid bacteria to carry out lactic fermentation, can under the situation that does not lose the hemoglobinase function, make organic feed stripped.
Lactic fermentation is to utilize lactic acid bacteria to carry out fermentation process, therefore needs sugar as nutrient.And produce metabolite lactic acid.Metabolism is mainly undertaken by decomposing sugar, and not decomposing protein and fat.That is to say that one-tenth that blood is separated out is divided into the nutrient source of lactic acid bacteria propagation, biochemistry decompose mainly to as if sugar.
Hemoglobinase in the blood in the lactic acid bacteria culture solution just can not be decomposed and together left behind with protein and fat as a result.
But along with lactic fermentation ground carrying out,, the lactic acid of generation causes solidifying of protein in the blood thereby will reducing the solution pH value.And then the water-fast calcium lactate of the reaction of the calcium ion in lactic acid and blood formation, thereby promote solidifying of protein.
And in the fermentation process, the symbiotic fermentation of lactic acid bacteria and phototrophic bacteria is more effective.Phototrophic bacteria breeds as nutrient source with ammonia, hydrogen sulfide, carbonic acid, hydrogen ion.If in zymotic fluid, add the phototrophy cell, thereby then can produce glucose as nutrient source with hydrogen ion and the carbonic acid that produces in the lactic fermentation process.This glucose becomes the nutrient source of lactic acid bacteria again, and lactic acid bacteria absorbs nutrition and produces lactic acid from the glucose that phototrophic bacteria produced.The symbiotic fermentation of lactic acid bacteria and phototrophic bacteria has increased the generation of lactic acid like this.
In the lactic fermentation process of blood, the growing amount of lactic acid increases and promotes solidifying of protein, and just separating of protein and hemoglobinase has been very easy to.Phototrophic bacteria will produce biochemical reaction must the energy light source, and the symbiotic fermentation of lactic acid bacteria and phototrophic bacteria needs the light irradiation.
Be described as follows in this manufacture method with regard to employed above-mentioned high concentration lactic acid bacteria dry ingredient among the present invention.In the flow chart as shown in Figure 1, the A operation is the pure cultivation operation of lactobacillus fermenti.1 liter of pure water (Purified Water) glucose 5g for example wherein, yeast 5g starch 5g mix and put into autoclave, add lactobacillus fermenti 50ml, cultivate 48 hours down for 40 ℃ in incubator.Thereby obtain the pure nutrient solution of lactobacillus fermenti.
The B operation is to add the operation that skim milk, syrup and starch, defatted soy flour etc. mix in the resulting pure nutrient solution in Purified Water and A operation.
Concrete method is as follows, and lactobacillus fermenti is trained liquid 1.5%, skim milk 4%, natural salt 0.5-1.5%, (deceiving) syrup 1%, glutamic acid 0.5%, starch 2%-6%, defatted soy flour 3%-9%, Purified Water 100% purely.The B operation is a mixed processes.The percentage of Chu Xianing all refers to percentage by weight in the present invention.
In other words, in the water of 10 liters (10kg), mix above-mentioned pure nutrient solution 150ml (15g), skim milk 400g, natural salt 50g-150g, (deceiving) syrup 100g, glutamic acid 50g, starch 200-600g, defatted soy flour 300g-900g.
The C operation is the operation that this mixed liquor is stirred, and mixes fully.Ensuing D operation is that propagation is cultivated operation, for example, is incubated 48 hours down at 40 ℃.Carrying out the propagation of lactic acid bacteria handles.
In E operation thereafter, utilize mixer slurrying.(agitating procedure)
Thereafter F operation is an operation (efflorescence operation) of utilizing drying machine to spray and dry.Specify as followsly, drying machine is set 150 ℃-180 ℃ of inlet temperatures, 75 ℃-80 ℃ of outlet temperatures.The oven dry of spraying finally obtains the dry powder of lactic acid bacteria composition (dry powder of lactic acid bacteria composition) of average diameter 1 μ m-9 μ m.Use the micronize structure spray-drying installation that can form single micron spray droplet.
Enlarge the Unit Weight surface area of spray droplet with this, thereby effectively air is dried in contact, when removing moisture (the dry processing) rapidly, reduce as far as possible because the thalline that the temperature of oven dry air causes damages and be dead.And in the drying process of above-mentioned single micron spray droplet, protein and carbohydrate form diaphragm at outer surface, thereby the inner thalline of more effective protection prevents damage and dead.
Lactobacillus fermenti quantity reaches 1.1 * 10 in the lactic acid bacteria dried powder 9-1.2 * 10 10Cfu/g and powder average particulate diameter 1 μ m-9 μ m.Particle surface is covered by starch and carbohydrate diaphragm in addition.
Following table 1-table 6 has been showed the embodiment 1-embodiment 6 of lactic acid bacteria dried powder.Promptly change the weight ratio of each composition among Fig. 1 mixed processes B, thereby influence propagation is thereafter cultivated the different measurement result (thalline number) of step D generation.
[table 1]
(embodiment 1)
Pure nutrient solution 150ml(1.5%)
Skimmed milk 400g(4%)
Mineral salt 50g(0.5%)
Black honey 100g(1%)
Glutamic acid soda 50g(0.5%)
Starch 200g(2%)
Defatted soy flour 300g(3%)
Measurement result 1.1×10 9cfu/g
[table 2]
(embodiment 2)
Pure nutrient solution 150ml(1.5%)
Skimmed milk 400g(4%)
Mineral salt 100g(1%)
Black honey 100g(1%)
Glutamic acid soda 50g(0.5%)
Starch 400g(4%)
Defatted soy flour 300g(3%)
Measurement result 8.9×10 9cfu/g
[table 3]
(embodiment 3)
Pure nutrient solution 150ml(1.5%)
Skimmed milk 400g(4%)
Mineral salt 150g(1.5%)
Black honey 100g(1%)
Glutamic acid soda 50g(0.5%)
Starch 600g(6%)
Defatted soy flour 300g(3%)
Measurement result 1.4×10 9cfu/g
[table 4]
(embodiment 4)
Pure nutrient solution 150ml(1.5%)
Skimmed milk 400g(4%)
Mineral salt 150g(1.5%)
Black honey 100g(1%)
Glutamic acid soda 50g(0.5%)
Starch 600g(6%)
Defatted soy flour 500g(5%)
Measurement result 8.9×10 9cfu/g
[table 5]
(embodiment 5)
Pure nutrient solution 150ml(1.5%)
Skimmed milk 400g(4%)
Mineral salt 150g(1.5%)
Black honey 100g(1%)
Glutamic acid soda 50g(0.5%)
Starch 600g(6%)
Defatted soy flour 700g(7%)
Measurement result 1.2×10 9cfu/g
[table 6]
(embodiment 6)
Pure nutrient solution 150ml(1.5%)
Skimmed milk 400g(4%)
Mineral salt 150g(1.5%)
Black honey 100g(1%)
Glutamic acid soda 50g(0.5%)
Starch 600g(6%)
Defatted soy flour 900g(9%)
Measurement result 8.9×10 9cfu/g
In table 1-table 6, measurement result (thalline number) test method is as follows.
At first test material 0.1g and 0.2g are dissolved in the physiological saline of 10ml.After 10 times of stoste dilutions, hybrid standard agar medium 1ml.Cultivated 16-24 hour down at 37 ℃.Thereby the colony of measuring appearance obtains the thalline quantity in the 1ml test material.
Among the foregoing description 1 (table 1) and embodiment 2 (table 2) and the embodiment 3 (table 3), the addition of natural salt and starch is different.Wherein the thalline quantity among the embodiment 2 is maximum, and highly stable.
In addition among the foregoing description 4 (table 4) and embodiment 5 (table 5) and the embodiment 6 (table 6), defatted soy flour go into the amount difference, the comparative measurements result is as can be known among embodiment 5 (table 5) and the embodiment 6 (table 6), the addition difference of defatted soy flour, comparative measurements result embodiment 5 as can be known obtains maximum thalline numbers, reaches 1.2 * 10 10Cfu/g.Thalline number among embodiment 4 and the embodiment 6 is 8.9 * 10 9Cfu/g, quantity also belongs to well.
Among the foregoing description 1-6, lactobacillus fermenti quantity has all reached 1.1 * 10 9-1.2 * 10 10 Cfu/gStandard.
By the foregoing description 1-6 as can be known, though lactic acid bacteria and thermo-labile is not suitable for powder process.But, can stand the temperature of spray-drying installation owing to be subjected to the protection of the diaphragm of starch and carbohydrate formation.And thalline quantity has reached 1.1 * 10 9-1.2 * 10 10 Cfu/gThereby high-purity make single thalline lactic acid bacteria powder.(this method also is applicable to the mushroom that other hear resistances are low).In addition owing to be thereby that powder can stop thalline activity long preservation.The hear resistance of powder and acid resistance, alkali resistance all are improved behind the covered with protective film, particularly can stablize to keep highly purified thalline quantity.
The spray-drying installation inlet temperature is set at 150 ℃-180 ℃, and it is in order to obtain abundant thalline quantity and to carry out abundant drying at short notice that outlet temperature is set at 75 ℃-87 ℃.If inlet temperature less than 75 ℃, can cause drying efficiency low less than 150 ℃, outlet temperature, the index of aridity surpasses 7%.If instead inlet temperature surpasses 180 ℃, outlet temperature surpasses 87 ℃, can cause thalline survival rate low (being lower than 1/10th).
Above-mentioned manufacture process can fully reduce death and the damage of lactic acid bacteria and keep high-survival rate.Manufacturing process is simple in addition, and can stablize the dry powder of lactic acid bacteria that obtains abundant drying.
Can access lactobacillus fermenti thalline several 1.1 * 10 9-1.2 * 10 10The high-survival rate lactic acid bacteria dry ingredient of cfu/g.Make, technology is also very easy, does not need main equipment.
And powder is covered by starchiness and carbohydrate diaphragm, and is heat-resisting, acidproof, alkaline resistance properties is good.
At this, go on to say the manufacture method of the feed stripped that proposes among the present invention.At first describe with regard to separation circuit.In this operation, hemoglobinase is included in the solution in the lactic fermentation liquid of blood, thereby by zymotic fluid is carried out the aqueous solution that Separation of Solid and Liquid obtains containing hemoglobinase.
Owing to the cytoplasm that contains blood in the blood after the lactic fermentation, solidifying protein, calcium lactate, fat etc. are changed the shape material, for removing solid content in the zymotic fluid by centrifugation, membrane filter, filter membrane etc. carries out that Separation of Solid and Liquid is handled rather than with the method for natural sedimentation.
Particularly membrane filter is selected the different character that sees through liquid with filter membrane by the distinguishing characteristic of filter membrane.The solidifying protein that zymotic fluid obtains after by centrifugation and the cytoplasm of blood separate, and contain mineral matter, hemoglobinase, fat and water miscible organic matter in parting liquid.And in the liquid that zymotic fluid process membrane filter obtains, fat constituent is removed, contain mineral matter, hemoglobinase and water miscible organic matter, the solution that obtains in the processing through filter membrane contains low molecule organic matters such as mineral matter, hemoglobinase and organic acid.
Next just adhere to drying process and describe, this operation is divided two parts, and a part is the operation of adhering to operation and the dry material that is adsorbed onto from parting liquid that resulting parting liquid in the above-mentioned separation circuit is worked by material (attachment) adhewsive action.
Because the lactic fermentation liquid in the blood after the Separation of Solid and Liquid, unstable on biochemistry, it is very easily putrid and deteriorated to run into extraneous air.
Therefore adhere in the operation, for making the parting liquid (zymotic fluid) of removing solid content more stable aspect the physical property.Use porous adsorbate-zeolite-adsorb.Parting liquid after the absorption has physics stability thus, and the weight ratio of attachment and parting liquid is advisable with 30%--60%.
The size of attachment particle (average particulate diameter) 1mm-5mm.If when not reaching lower limit, surface area is more for a short time, and to adhere to the adhesive rate of parting liquid low, and it is just too big, not too suitable as feed to surpass the words of the upper limit.
Therefore the composition of the parting liquid that obtains after the attachment absorption in drying process has physical stability.Owing to separate also easy spoiling attack thalline, be easy to corruption.For preventing this situation, dry attachment.
Use drying machine in the drying process, can adopt air dry and heated drying dual mode.Adsorb in the attachment, become the stable in properties of hemoglobinase aspect physics, biochemistry of drying regime, thereby become the feed that to bring into play hemoglobinase for a long time.
The concrete grammar that adheres to dry engineering is installed attachment at first in the plane, utilizes air dry by sprayer ejection parting liquid.Obtain the attachment in the parting liquid thus, and utilize attachment to absorb naturally.(on the weight) can obtain the uptake more than 2 times.
And the words uptake of utilizing aerosol type pneumatic drier drying 4 times when being air dry.
Next just utilize the dry thing of above-mentioned high concentration lactic acid bacteria to obtain domestic animal and use the concrete of feed
Embodiment describes
The pig blood that will obtain from the slaughterhouse heated about 55 ℃ about 4 hours, was cooled to then that lactobacillus fermenti quantity reaches 1.1 * 10 in 40 ℃ of lactic acid bacteria dried powders 9-1.2 * 10 10Cfu/g and powder average particulate diameter 1 μ m-9 μ m.It is kept at 40 ℃ carried out lactic fermentation in 72 hours.Zymotic fluid is filtered by membrane filter, in filtrate, add weight ratio and make an appointment with the synthetic zeolite of half to use air drier to carry out drying.Thus, zeolite 1g can obtain the zeolite adsorption thing that 88mg contains hemoglobinase, thereby obtains feed stripped.
As mentioned above, according to the present invention, think the blood of the pig ox that always all discards in the slaughterhouse always, carry out symbiotic fermentation by adding lactic acid sclerotium phototrophic bacteria, according to same-action not is that it extract from blood, so will be from the fermentate to the solidifying protein etc. solid content carry out Separation of Solid and Liquid.This parting liquid obtains the high-quality feed stripped attached on the zeolite by drying.The animal blood of always all discarding thus determines final processing method after being necessary to handle again.
Among the present invention, be 1.1 * 10 as mentioned above with density 9-1.2 * 10 10The lactobacillus fermenti of cfu/g is made the dry composition of lactic acid bacteria with the powder of average particulate diameter 1-9 μ m, mixes after animal blood ferments, and obtains dry domestic animal and uses feed thereby zymotic fluid is carried out Separation of Solid and Liquid and the dry attachment of adsorbing separation liquid.Make easyly, feeding quality is stable, and domestic animal can be kept good health status behind the feed that obtains of throwing something and feeding, and grows smoothly, and the body weight increase significantly improves.And delicious meat, best in quality.
This feed can keep off infection and other diseases, and can reduce the use of excessive medicines such as antibiotic input, improves meat.
Though sharp and blood but be not allow its corrupt but obtain can long preservation feed.
The feed stripped manufacture method that proposes according to the present invention is 1.1 * 10 with density 9-1.2 * 10 10The lactobacillus fermenti of cfu/g is made the dry composition of lactic acid bacteria with the powder of average particulate diameter 1-9 μ m, carry out fermentation procedure for mixing animal blood, supply with an amount of air and carry out the interior organic matter of fermentation process decomposition blood, keep the biochemistry stability of blood, though utilized blood be not allow its corrupt but obtain can long preservation feed.
And with density is 1.1 * 10 9-1.2 * 10 10The lactobacillus fermenti of cfu/g is made the dry composition of lactic acid bacteria with the powder of average particulate diameter 1-9 μ m, mixes after animal blood ferments, and obtains dry domestic animal and uses feed thereby zymotic fluid is carried out Separation of Solid and Liquid and the dry attachment of adsorbing separation liquid.Feed is made easy, steady quality.The domestic animal of throwing something and feeding can be kept good health status, grows smoothly, and the body weight increase significantly improves.And delicious meat, best in quality.And can obtain to treat the infectious disease of domestic animal, improve the feed of meat function.

Claims (2)

1, a kind of domestic animal is used the feed manufacture method, it is characterized in that: with density is 1.1 * 10 9-1.2 * 10 10The lactobacillus fermenti of cfu/g is made the dry composition of lactic acid bacteria with the powder of average particulate diameter 1-9 μ m, after the mixing animal blood ferments, zymotic fluid is carried out Separation of Solid and Liquid and the dry thing of adsorbing separation liquid, uses feed thereby obtain dry domestic animal.
2, use the feed manufacture method by the described domestic animal of claim 1, it is characterized in that:
(1) blood of domestic animal carries out heat treated earlier, and heating-up temperature is 50 ℃-75 ℃, and be 1-5 hour heat time heating time;
(2) weight ratio between dry composition of fermentation procedure middle and high concentration lactic acid bacteria and blood is 0.03%-0.7%, fermentation temperature is 30 ℃-43 ℃, fermentation time is 48-72 hour, zymotic fluid is filtered by membrane filter, adding half synthetic zeolite of weight ratio in filtrate uses air drier to carry out drying, thus, the 1g zeolite obtains the zeolite adsorption thing that 88mg contains hemoglobinase, thereby obtains feed stripped.
CNB2004100061919A 2004-01-14 2004-03-08 Fodder special for livestock and manufacturing method thereof Expired - Fee Related CN1233261C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2004006907A JP4101769B2 (en) 2004-01-14 2004-01-14 Livestock feed and method for producing the same
JP2004006907 2004-01-14

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CN1557183A CN1557183A (en) 2004-12-29
CN1233261C true CN1233261C (en) 2005-12-28

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105580987B (en) * 2014-10-23 2019-08-30 麦闪石G.M.株式会社 Feed organic coating Porous particle and preparation method thereof
KR102186526B1 (en) * 2019-01-11 2020-12-03 주식회사 에스씨아이 Manufacturing method of feed additive for potentiating immune response using slaughter blood, feed additive for livestock manufactured therefrom, and feed comprising the same
KR102187522B1 (en) * 2020-05-18 2020-12-08 주식회사 삼다 Method for Preparing Fermented Porcine Blood and a Feed Composition for poultry Using the Same

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