KR102186526B1 - Manufacturing method of feed additive for potentiating immune response using slaughter blood, feed additive for livestock manufactured therefrom, and feed comprising the same - Google Patents

Abstract

The present invention is a method for mass-producing feed additives with enhanced immune activity by effectively separating and concentrating immune enhancing proteins by treating plasma separated from livestock blood through an ultrafiltration process, and feed additives prepared accordingly and It relates to feed for livestock containing. To this end, the present invention is a method of preparing a feed additive for enhancing immunity using slaughter blood, comprising the steps of collecting the slaughter blood of pigs, adding an anticoagulant, and mixing (S1); Centrifuging the slaughtered blood to obtain plasma (S2); A first filtration step (S3) of ultrafiltration of the plasma to obtain a filtrate from which a material having a molecular weight exceeding 200 kilodaltons (kDa) is removed; Secondary filtration step obtained by ultrafiltration of the filtrate to separate a first fraction having a molecular weight of more than 100 kilodaltons (kDa) to 200 kilodaltons (kDa) or less and a second fraction having a molecular weight of 100 kilodaltons (kDa) or less (S4); And a step (S5) of separating and obtaining solids from the first and second fractions, respectively; and a method for preparing a feed additive for enhancing immunity using slaughtered blood, and a feed additive prepared by the method. Provide feed for livestock containing this. Accordingly, the present invention can reduce the cost of slaughter blood disposal, prevent environmental pollution, reduce the cost of feed antibiotics, prevent antibiotic abuse, and contribute to increase the productivity of livestock as a new protein feed resource and improve the quality of livestock products.

Description

Manufacturing method of feed additives for enhancing immunity using slaughter blood, feed additives prepared accordingly, and feed for livestock containing the same. THE SAME}

The present invention is a method for mass-producing feed additives with enhanced immune activation function by effectively separating and concentrating immune enhancing proteins by treating plasma separated from pig slaughter blood through an ultrafiltration process, and feed additives prepared accordingly, and It relates to feed for livestock containing this.

Animal feed, unlike vegetable feed, has high protein supply ability and excellent amino acid composition in terms of nutrition, making it an efficient feed resource for producing high-quality protein food.

Animal feeds currently produced and supplied in Korea include meat and bone meal, resin meal, feather meal, poultry slaughter by-products (chicken meal), blood meal, and single cell protein, as representative fish meal. Domestic production and consumption of feed are decreasing.

In addition, as the outbreak of mad cow disease expands negative views on animal diseases of animal protein feed, it is affecting not only ruminants but also non-ruminants in Europe, and the resource value of animal protein feed is seriously threatened.

Fortunately, because mad cow disease has not occurred in Korea, the impact on feeding non-ruminant animal feed has not yet been greatly affected, but as livestock consumers are not slowing down, it is becoming more difficult to secure protein resources.

Moreover, as the demand for vegetable protein resources is increasing rapidly, mainly in Europe, the price is also skyrocketing, and the demand for the development of new protein feed resources continues to increase.

It has been known for a long time that livestock with poor nutritional status generally suffer from diseases due to decreased resistance and high mortality, and that livestock with good nutritional status have strong resistance to pathogens.

Therefore, there are many clinical epidemiologic studies that believe that nutritional status affects the immune system and support it. The nutrition-immune-infection relationship is very complex, and the variables involved are very diverse.

Malnutrition livestock generally have a bad environment inside the barn, and infection with diseases is always an accompanying problem for these livestock. In other words, malnutrition, decreased immunity, and infection are affected by each other, and the environment inside the shed is thought to be the basic cause. Poorly malnourished livestock are susceptible to disease, and a vicious cycle continues to worsen nutritional status when diseased.

Commercially available polyclonal antibodies are usually obtained from the sera of mammals such as mice, rabbits, sheep, goats, horses, pigs, and cattle after immunization. However, since these antibodies cannot be manufactured on an industrial scale, they have a disadvantage in that they are not cost effective. This is because it is difficult to obtain blood, that is, serum, and a lot of attention must be paid to collecting blood.

Recently, colostrum from mammals secreted a few days after birth is attracting attention from the viewpoint of antigen-specific antibodies. However, this method is also extremely limited in producing antibodies, since the production period of colostrum is only a few days and the pure separation process is very complicated.

The domestic pig slaughter volume is 12 million heads per year, and the amount of blood bleeding reaches 36,000 tons. To deal with this, an annual cost of 100 million won per slaughterhouse is required, which is pointed out as a major factor that adversely affects the net income of the slaughterhouse.

Slaughter blood is a major report of immune substances and can be used in various ways because it contains a large amount of physiologically active substances. If the supply of antibodies is convenient and large-scale production is possible, it can be said that it is desirable to produce a large amount of antibodies through special applications because it can promote the use of antibodies not only in pharmaceuticals, but also in food and cosmetics industries.

On the other hand, as a conventional technology to utilize livestock blood, which is a waste slaughter by-product, in Korean Patent Application Laid-Open No. 10-2008-0040296, the blood of livestock is sterilized and processed to mix organic and inorganic substances and microbial preparations. A technology related to fertilizer and feed for the production of agricultural and livestock products capable of preventing and treating iron deficiency and anemia in animals is disclosed.

In addition, Korean Patent Laid-Open No. 10-2013-0031402 includes a blood component-added fungus culture prepared by pulverizing a solid culture obtained by culturing fungi by adding blood meal prepared by sterilizing livestock blood to mushroom waste medium. A feed additive composition for enhancing immunity is disclosed, and Korean Patent Application Laid-Open No. 10-2014-0111759 includes a pretreatment step of decomposing the collected slaughter blood by ultrasonic treatment; An enzymatic decomposition step of injecting an enzymatic decomposition fermenting agent into the slaughtered blood degraded in the pretreatment step; And a solid-liquid separation step of separating the slaughtered blood that has undergone the enzymatic decomposition step, and a method for producing an amino acid liquid manure and protein dry feed using slaughtered blood is disclosed.

Furthermore, Korean Patent Registration No. 10-1573712 includes a fermentation step in which the collected slaughter blood is contained, stirred, and then heated to ferment the protein contained in the slaughtered blood to convert it into amino acids; A steaming step of agitating the fermented slaughtered blood and directly spraying hot steam to steam amino acids contained in the fermented slaughtered blood into an amorphous mass having multiple pores; A dehydration step of separating the steamed amorphous amino acid mass and residual moisture; Molding step of pressing the dehydrated amorphous amino acid mass into a granular state in the form of granules or pellets; And a drying step of drying the molded granular amino acid by irradiation with hot air and microwaves; a method for producing a granular amino acid feed and fertilizer using slaughtered blood is disclosed.

Republic of Korea Patent Publication No. 10-2008-0040296 (published on May 8, 2008) Republic of Korea Patent Publication No. 10-2013-0031402 (published on March 29, 2013) Republic of Korea Patent Publication No. 10-2014-0111759 (published on September 22, 2014) Korean Patent Registration No. 10-1573712 (announced on December 11, 2015)

The present invention prevents environmental pollution and increases livestock productivity by effectively separating and concentrating immune enhancing proteins from the blood of pigs that are discarded as slaughter by-products to produce feed additives with enhanced immune activity and feed for livestock in large quantities. It is an object of the present invention to provide a method of manufacturing a feed additive that can contribute to the improvement of the quality of livestock and livestock products, a feed additive prepared accordingly, and feed for livestock containing the same.

In order to achieve the above object, the present invention is a method of manufacturing a feed additive for enhancing immunity using slaughter blood, comprising the steps of collecting the slaughter blood of pigs, adding an anticoagulant, and mixing (S1); Centrifuging the slaughtered blood to obtain plasma (S2); A first filtration step (S3) of ultrafiltration of the plasma to obtain a filtrate from which a material having a molecular weight exceeding 200 kilodaltons (kDa) is removed; Secondary filtration step obtained by ultrafiltration of the filtrate to separate a first fraction having a molecular weight of more than 100 kilodaltons (kDa) to 200 kilodaltons (kDa) or less and a second fraction having a molecular weight of 100 kilodaltons (kDa) or less (S4); And a step (S5) of separating and obtaining a solid content from the first and second fractions, respectively, wherein the solid content of the second fraction is, in the second fraction obtained in the second filtration step (S4), acetone And 1.6 M hydrochloric acid in a ratio of 8:2 (v/v), acidic acetone was added to denature the protein, followed by centrifugation to obtain a precipitate, and distilled water was added to the precipitate. It provides a method for producing a feed additive for enhancing immunity using slaughtered blood, characterized in that obtained by removing residual acetone by distilling under reduced pressure after protein regeneration (renaturation).

Here, the manufacturing method is a step of preparing a liquid feed additive by adjusting the solid content of the first fraction to a concentration of 10% (w/v) using a 0.9% concentration (w/v) sodium chloride (NaCl) solution. (S6); may further include.

In addition, the manufacturing method is prepared by adjusting the solid content of the second fraction to a concentration of 25% (w/v) using a 0.9% concentration (w/v) sodium chloride (NaCl) solution to prepare a liquid feed additive. Step (S6'); may further include.

In addition, the present invention provides a feed additive prepared according to the method.

In addition, the present invention provides a feed for livestock containing the feed additive.

According to the present invention, by effectively recovering and concentrating immune proteins from slaughter blood that is generated and discarded in large quantities in the slaughtering process of livestock, it is possible to prepare a feed additive for enhancing immunity and feed for livestock containing the same, It has an excellent effect that can contribute to increase the productivity of livestock and improve the quality of livestock as a new protein feed resource, reducing the cost of slaughter blood disposal, preventing environmental pollution, reducing the cost of antibiotics for feed, and preventing antibiotic abuse.

1 is a flow chart illustrating a method of manufacturing a feed additive for enhancing immunity using slaughtered blood according to an embodiment of the present invention.
Figure 2 is a flow chart illustrating a method of manufacturing a feed additive for enhancing immunity using slaughtered blood according to another embodiment of the present invention.
3 is a photograph showing the form and conditions of a cage used in a broiler specification experiment.
Figure 4 is a graph showing the analysis of the solid content in pig plasma.
5 is a graph showing the analysis of constituents of pig plasma before and after drying.
Figure 6 is a graph showing the molecular weight analysis of the peptide present in pig plasma.
Figure 7 is a data measured by the weight of broiler breeding in the first half.
Figure 8 is data obtained by measuring the weight of broiler breeding in the second half.
Figure 9 is a data survey of feed requirements throughout broiler breeding.
10 is a photograph of the actual state of the broiler specification experiment.

The present invention may be implemented in various different forms and is not limited to the embodiments described herein. The terms used in the present invention are only used to describe specific embodiments, and are not intended to limit the present invention. In addition, unless otherwise defined, all terms, including technical or scientific terms, used herein have the same meaning as commonly understood by a person of ordinary skill in the art to which the present invention belongs. Hereinafter, an embodiment of a method for preparing a feed additive for enhancing immunity using slaughtered blood according to the present invention will be described in detail.

1 and 2 are flowcharts each illustrating a method of manufacturing a feed additive for enhancing immunity using slaughtered blood according to an embodiment of the present invention.

1 and 2, the method of manufacturing a feed additive for enhancing immunity using slaughtered blood according to the present invention includes a slaughter blood collection step (S1), a plasma acquisition step (S2), and a first ultrafiltration step (S3). , A second ultrafiltration step (S4), and a solid content obtaining step (S5).

1. Slaughter Blood Collection Step (S1)

This is the step of collecting slaughter blood from pigs that are discarded from the slaughterhouse. As soon as the slaughtered blood is collected, EDTA is added as an anticoagulant and then slowly mixed. It is desirable that the final concentration of EDTA added to slaughtered blood is 0.5% (w/v). Slaughter blood mixed with anticoagulants can be frozen, refrigerated or stored at room temperature as needed, such as transport, until the next stage of processing. However, in order to prevent the propagation of microorganisms or denaturation of proteins, it is preferable to store them frozen or refrigerated, but in view of the economical aspect, it is preferable to store them refrigerated in consideration of the cost of freezing and thawing.

2. Plasma obtaining step (S2)

This is a step of separating and obtaining plasma from the slaughtered blood prepared in step (S1). When the slaughtered blood is centrifuged, plasma and blood cells are separated in a volume ratio of approximately 5:5, of which only plasma is obtained separately. A continuous tube type centrifuge may be used for centrifugation, and it is preferable to perform continuous centrifugation at about 12,000 rpm.

3. The first ultrafiltration step (S3)

This is a step of obtaining a filtrate from which substances exceeding a certain molecular weight are removed by ultrafiltration of the plasma obtained in step (S2). The first ultrafiltration membrane was obtained by separating a filtrate from which a material having a molecular weight exceeding 200 kDa was removed by setting the Molecular Weight of Cut-off to 200 kilodaltons (kDa).

4. Second ultrafiltration step (S4)

In this step, the filtrate obtained in step (S3) is secondarily ultra-filtered to separate and obtain a substance having a specific molecular weight range. The fractional molecular weight of the secondary ultrafiltration membrane is 100 kDa, and each is obtained by separating into a material having a molecular weight of more than 100 kDa to 200 kDa (first fraction) and a material having a molecular weight of 100 kDa or less (second fraction) (second fraction).

5. Solid content acquisition step (S5)

This is a step of separating and obtaining solids from the first fraction (molecular weight of more than 100 kDa to 200 kDa or less) and the second fraction (molecular weight of 100 kDa or less) obtained in step (S4). Methods such as precipitation, centrifugation, concentration, and drying may be used to separate the solids.

For example, the solid content of the second fraction is acetone and 1.6 M hydrochloric acid in a ratio of 8:2 (v/v) to the second fraction (molecular weight 100 kilodalton or less) obtained in the second ultrafiltration step (S4). After denaturing the protein by adding the mixed acidic acetone, continuous centrifugation at 12,000.rpm to obtain a precipitate, and a small amount of distilled water was added to the precipitate to regenerate the protein, and then reduced pressure. It can be obtained by distilling in a still to remove residual acetone.

6. Feed additive manufacturing step (S6, S6')

On the other hand, the method of manufacturing a feed additive for enhancing immunity using slaughter blood according to the present invention may further include a feed additive manufacturing step (S6, S6').

For example, the solid content of the first fraction can be prepared as a liquid feed additive by adjusting the concentration to 10% (w/v) using a 0.9% concentration (w/v) sodium chloride (NaCl) solution (S6). .

In the case of the solid content of the second fraction, it can be prepared as a liquid feed additive by adjusting the concentration to 25% (w/v) using a 0.9% concentration (w/v) sodium chloride (NaCl) solution (S6'). .

The method of manufacturing a feed additive for enhancing immunity using slaughter blood according to the present invention further includes a product packaging step of dividing the feed additive prepared through the feed additive manufacturing step (S6, S6') into a predetermined weight unit and packaging it. I can.

In addition, according to an embodiment of the present invention, it is possible to prepare a feed for livestock containing the feed additive by mixing the feed additive prepared by the above-described method into a blended feed for livestock.

Hereinafter, examples of the feed additive for enhancing immunity using slaughter blood according to the present invention will be described in more detail. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited thereto.

<Example 1> Preparation of feed additive for enhancing immunity using the first fraction

EDTA was added to the collected pig slaughter blood so that the final concentration was 0.5% (w/v). The slaughtered blood was centrifuged at 12,000 rpm using a continuous tube type centrifuge, separated into plasma and blood cells, and then obtained by separating only plasma. The obtained plasma was first ultra-filtered to obtain only the filtrate from which a substance having a molecular weight exceeding 200 kilodalton was removed. The obtained filtrate was separated into a material having a molecular weight of more than 100 kilodaltons and less than 200 kilodaltons (the first fraction) and a material having a molecular weight of 100 kilodaltons or less (the second fraction) using an ultrafiltration membrane having a molecular weight cutoff of 100 kilodaltons. Obtained. Thereafter, the first fraction was concentrated and dried to separate and obtain a solid. The solid content was adjusted to a concentration of 10% (w/v) using a 0.9% concentration (w/v) sodium chloride solution to prepare a feed additive in a liquid form.

<Example 2> Preparation of a feed additive for enhancing immunity using the second fraction

Acetone and 1.6 M hydrochloric acid in a ratio of 8:2 (v/v) were added to the material (second fraction) having a molecular weight of 100 kilodaltons or less obtained in Example 1, followed by continuous centrifugation to precipitate a precipitate. And distilled water was added to the precipitate, followed by distillation under reduced pressure to remove residual acetone to obtain a solid content. The solid content was adjusted to a concentration of 25% (w/v) using a 0.9% concentration (w/v) sodium chloride solution to prepare a feed additive in a liquid form.

<Experimental Example>

1. Test content and method

end. Determination of molecular weight

Protein molecular weight was determined using MALDI-TOF.

I. Pig specification experiment

This experiment was conducted at a batch of 2,000 pigs breeding farm in Juksan, Anseong. Eight sows were delivered per week, and about 90 piglets weaned from sows were disclosed per week, and a total of 30 control animals were tested in 3 replicates. Thus, this experiment was carried out with a total of 270 weaned piglets with 90 control heads, 90 heads for feed additive administration using the first fraction, and 90 heads for feed additive administration using the first fraction.

(1) Salary for piglets

As for the administration of piglets, 3ml was given within 1 hour after the piglets were born, and 3ml was forcibly administered by syringe every day until 7 days of age, and diarrhea frequency and weight were measured until weaning at 25 days of age.

(2) Salary for sows

For the administration of sows, 2 kg per ton of feed was fed from 2 weeks before delivery, and 4 kg per ton of feed was fed until lactation after delivery.

All. Broiler specification experiment

Commercially available feed was fed, and this experiment was performed on a total of 300 broiler chicks. The feed additive administration group using the first fraction, the feed additive administration group using the second fraction, and the control group were separated and reared in 20 numbers, repeated 5 times, and reared up to 32 days of age to investigate the weight and feed efficiency. The cage environment was carried out at an experimental farm attached to SCI Co., Ltd. in Seosan City, Chungcheongnam-do as a house cage, and the form and conditions of the cage are as shown in FIG. 3. The actual state of the broiler specification experiment is shown in the photograph of FIG. 10.

2. Test result

end. Distribution of the composition of pig blood

Slaughtered pig blood was provided from Hongju Meat (Hongseong-gun, Chungcheongnam-do), and in the process of processing, EDTA was added to a final concentration of 0.5% (w/v) after receiving blood directly from the neck of the slaughtered pig, and then slowly mixed and iceboxed. And transferred to the laboratory for analysis. Porcine blood was centrifuged at 4,000 rpm for 5 minutes in a batch type, separated into plasma and blood cells, and analyzed. As a result, blood cells and plasma were almost 5:5 by volume.

FIG. 4 is data obtained by analyzing the solid content in pig plasma, and FIG. 5 is data obtained by analyzing components of pig plasma before and after drying. The solid content in plasma was 8.9% by weight and the water content was 91.1% by weight. And the protein content was 7.59% by weight.

6 is data obtained by examining the molecular weight of peptides present in pig plasma. By weight, less than 5 kDa was 2.538%, 5 to 10 kDa is 0.054%, 10 to 30 kDa is 0.019%, 30 to 50 kDa is 0.054%, 50 to 100 kDa is 90.393%, and 100 to 200 kDa is 4.03% , 200 kDa or more was 2.365%.

1 ton of blood was centrifuged to obtain a protein having a molecular weight of 100 to 200 kDa in plasma, and as a result, a dry amount of 1.78 kg could be obtained, and a protein having a molecular weight of 100 kDa or less could obtain 40.46 kg.

Most of the peptides with a molecular weight of 100 kDa or less were of Albumin ([Table 1]), and most of the peptides with a molecular weight of 100 to 200 kDa were of the γ-Globulin family of Ig G, Ig A, Ig D, and Ig E. Was ([Table 2]).

group Protein type Molecular Weight function Albumins Transthyretin 55~65 Transport route of Thyroxin and Triidothyronin Albumin 67 Osmotic pressure maintenance: transport path of fatty acids, bilirubin, bile acids, steroid hormones, pharmaceutical ingredients and inorganic ions

α1-Globulins Antitrypsin 51 Inhibition of trypsin and other proteolytic enzymes Antichymotrypsin 58~68 Inhibition of chymotrypsin Prothrombin 72 Thrombin precursor, aggregation factor II Transcortin 51 Cortisol, Corticosterone, and Progesterone transport Thytoxin-binding globulin 54 Transfer of Iodothyronins
α2-Globulins Antithrombin III 58 Inhibit blood clotting Plasminogen 90 Plasmin precursor, destroying blood clots Vitamin D-binding protein 52~59 Calciaol (Vitamin D3) transfer
β-Globulins Transferrin 80 Iron transfer Sex hormone binding globulin 65 Testoterone and Estradiol transfer Transcobalamin 38 Vitamin B12 transfer

group Protein type Molecular Weight function
γ-Globulins Ig G 150 Acquired antibodies Ig A 162 Mucosal protective antibody Ig D 172 β-lymphocyte receptors Ig E 196 Reagins (immediate reaction antibodies) α2-Globulins Ceruloplamin 135 Copper conveying β-Globulins C-reactive protein 110 Complementation

Table 3 is the data showing the results of feeding 3ml to sows within 1 hour after birth while feeding sows with additives from 2 weeks before delivery to weaning, and feeding 3ml daily with a syringe until 7 days of age.

Administration Control 1st fraction feed additive Second fraction feed additive Fresh Bean Count 30±0.6 30±0.6 30±0.5 Raw weight (kg) 1.43±0.13 1.42±0.12 1.42±0.13 Number of dead heads 0.5±0.2 1.2±0.8 2±1.3 Number of reasons 29.5±0.5 28.6±0.41 27±1.3 Weaning weight (kg) 11.4±1.08 10.24±1.28 9.83±1.3 Diarrhea 1.2±0.5 1.8±0.8 3±1.2

As a result of measuring the initial weight after adjusting the number of start feeding heads to about 30 heads for each treatment group, the feed additive administration group using the first fraction was 1.43±0.13kg, the feed additive administration group using the second fraction 1.42±0.12kg, the control group 1.42 It was ±0.13 kg. The number of dead heads until weaning was in the order of feed additive administration group using the first fraction (0.5±0.2), feed additive administration group using the second fraction (1.2±0.8), and control (2±1.3). Weaning weight was also 11.4±1.08kg, 10.24±1.28kg, 9.83±1.3kg respectively in the order of the feed additive administration group using the first fraction, the feed additive administration group using the second fraction, and the number of diarrhea heads respectively 1.2±0.5 , 1.8±0.8, 3±1.2 in order.

The mortality rate of the feed additive-administered group using the first fraction was lower than that of the other administration groups, the weaning weight was also higher than that of the other administration groups, and the number of diarrhea was significantly less.

7 is data obtained by measuring the weight of broiler breeding in the first half. The weight of broilers was measured for 2 weeks after the intestine. The feed additive administration group using the first fraction, the feed additive administration group using the second fraction, and the 14-day-old weight of the control group, respectively, were 352.31 g. It appeared in the order of 316.42g and 307.75g.

Figure 8 is the data of measuring the weight of broiler breeding in the second half, at 35 days of age, the feed additive administration group using the first fraction, the feed additive administration group using the second fraction, and the control group were respectively 1,697.51g, 1,698.08g and 1,432.63g.

Figure 9 is the data of the survey of feed requirements throughout the broiler breeding. The feed additive administration group using the first fraction, the feed additive administration group using the second fraction, and the control group showed 1.528kg, 1.52kg, and 1.705kg, respectively.

According to the above results, it is judged that the feed additive using the first fraction containing a large amount of immunoglobulin exhibits a lot of effects when the broiler is initially incubated, due to the high risk of exposure to diseases due to stress on the environment.

However, in the latter half of the year, there was no difference at all between the feed additive administration group using the second fraction and the feed additive administration group using the first fraction, but there was a remarkable difference from the control group.

Accordingly, it can be seen that it is desirable to feed the feed additive using the first fraction in the initial breeding of broilers and feed the feed additive using the second fraction in the second half.

As described above, the present invention effectively recovers and concentrates protein from slaughter blood that is generated and discarded in a large amount during the slaughter process of livestock, thereby producing a feed additive for enhancing immunity and a feed for livestock including the same, Not only can it reduce the cost of slaughter blood disposal, prevent environmental pollution, reduce the cost of antibiotics for feed, and prevent antibiotic abuse, but also contribute to increase the productivity of livestock and improve the quality of livestock products as a new protein feed resource.

In the above, preferred embodiments of the present invention have been described, but those skilled in the art to which the present invention pertains can be modified in that they can be implemented in other specific forms without changing the technical spirit or essential features of the present invention. You will see that there is. Therefore, the scope of the present invention is not limited to the described embodiments, and various modifications and variations can be made without departing from the spirit and scope of the present invention, and such modifications or variations are to be said to belong to the scope of the present invention. .

Claims (6)

As a method of manufacturing a feed additive for enhancing immunity using slaughter blood,
Collecting pig slaughter blood, adding an anticoagulant, and mixing (S1);
Centrifuging the slaughtered blood to obtain plasma (S2);
A first filtration step (S3) of ultrafiltration of the plasma to obtain a filtrate from which a material having a molecular weight exceeding 200 kilodaltons (kDa) is removed;
Secondary filtration step obtained by ultrafiltration of the filtrate to separate a first fraction having a molecular weight of more than 100 kilodaltons (kDa) to 200 kilodaltons (kDa) and a second fraction having a molecular weight of 100 kilodaltons (kDa) or less (S4); And
Including; separating and obtaining solids from the first and second fractions, respectively (S5),
The solid content of the second fraction,
To the second fraction obtained in the second filtration step (S4), acidic acetone obtained by mixing acetone and 1.6 M hydrochloric acid in a ratio of 8:2 (v/v) was added to denature the protein. Preparation of a feed additive for immunity enhancement using slaughter blood, characterized in that obtained by centrifugation to obtain a precipitate, and distilling under reduced pressure to remove residual acetone after adding distilled water to the precipitate to regenerate protein. Way. The method according to claim 1,
The solid content of the first fraction,
Slaughter blood, characterized in that it further comprises: preparing a feed additive in a liquid form by adjusting the concentration to 10% (w/v) using a 0.9% concentration (w/v) sodium chloride (NaCl) solution (S6) Method for producing a feed additive for enhancing immunity using. The method according to claim 1,
The solid content of the second fraction,
Slaughter, characterized in that it further comprises: preparing a feed additive in a liquid form by adjusting the concentration to 25% (w/v) using a 0.9% concentration (w/v) sodium chloride (NaCl) solution (S6'); A method of manufacturing a feed additive for enhancing immunity using blood. delete Feed additive prepared according to the manufacturing method of any one of claims 1 to 3. Animal feed comprising the feed additive of claim 5.

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