CN1225998C - Cigarette additive extracted from natural plant, and extracting method and use in cigarette thereof - Google Patents
Cigarette additive extracted from natural plant, and extracting method and use in cigarette thereof Download PDFInfo
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- CN1225998C CN1225998C CN 03140229 CN03140229A CN1225998C CN 1225998 C CN1225998 C CN 1225998C CN 03140229 CN03140229 CN 03140229 CN 03140229 A CN03140229 A CN 03140229A CN 1225998 C CN1225998 C CN 1225998C
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- extract
- cigarette
- plant
- coumarin derivative
- general coumarin
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- 235000019504 cigarettes Nutrition 0.000 title claims abstract description 92
- 239000000654 additive Substances 0.000 title claims abstract description 49
- 238000000034 method Methods 0.000 title claims abstract description 28
- 230000000996 additive effect Effects 0.000 title claims abstract description 17
- 239000000284 extract Substances 0.000 claims abstract description 80
- CFNMUZCFSDMZPQ-GHXNOFRVSA-N 7-[(z)-3-methyl-4-(4-methyl-5-oxo-2h-furan-2-yl)but-2-enoxy]chromen-2-one Chemical compound C=1C=C2C=CC(=O)OC2=CC=1OC/C=C(/C)CC1OC(=O)C(C)=C1 CFNMUZCFSDMZPQ-GHXNOFRVSA-N 0.000 claims abstract description 39
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 24
- 241000196324 Embryophyta Species 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229940067866 dandelion extract Drugs 0.000 claims abstract description 16
- 235000020691 dandelion extract Nutrition 0.000 claims abstract description 16
- 238000000605 extraction Methods 0.000 claims abstract description 16
- 239000001845 taraxacum officinale leaf extract Substances 0.000 claims abstract description 16
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 12
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 12
- 239000003960 organic solvent Substances 0.000 claims abstract description 9
- 238000005325 percolation Methods 0.000 claims abstract description 6
- 238000001953 recrystallisation Methods 0.000 claims abstract description 6
- 238000002386 leaching Methods 0.000 claims abstract description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 31
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 27
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 15
- 239000013078 crystal Substances 0.000 claims description 14
- 238000001035 drying Methods 0.000 claims description 14
- 239000002904 solvent Substances 0.000 claims description 14
- 241000208173 Apiaceae Species 0.000 claims description 12
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 12
- 241001093501 Rutaceae Species 0.000 claims description 10
- 235000021374 legumes Nutrition 0.000 claims description 10
- 239000000243 solution Substances 0.000 claims description 9
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 8
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims description 8
- 238000010992 reflux Methods 0.000 claims description 7
- PNEYBMLMFCGWSK-UHFFFAOYSA-N Alumina Chemical compound [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 claims description 6
- 241001529246 Platymiscium Species 0.000 claims description 6
- 240000004274 Sarcandra glabra Species 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 235000012054 meals Nutrition 0.000 claims description 6
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 6
- 235000010842 Sarcandra glabra Nutrition 0.000 claims description 5
- 241000245665 Taraxacum Species 0.000 claims description 5
- 230000001476 alcoholic effect Effects 0.000 claims description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 4
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 claims description 4
- HOEVRHHMDJKUMZ-UHFFFAOYSA-N Isofraxidin Chemical compound C1=CC(=O)OC2=C1C=C(OC)C(O)=C2OC HOEVRHHMDJKUMZ-UHFFFAOYSA-N 0.000 claims description 4
- ANCHXLMTFNOVDK-UHFFFAOYSA-N Isofraxidin Natural products COC1=C(O)C(OC)=CC2=C1OC=CC2=O ANCHXLMTFNOVDK-UHFFFAOYSA-N 0.000 claims description 4
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N benzo-alpha-pyrone Natural products C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 claims description 4
- -1 coumarin lactone Chemical class 0.000 claims description 4
- 239000003480 eluent Substances 0.000 claims description 4
- 244000061520 Angelica archangelica Species 0.000 claims description 3
- 235000001287 Guettarda speciosa Nutrition 0.000 claims description 3
- 244000296102 Selinum monnieri Species 0.000 claims description 3
- 239000000039 congener Substances 0.000 claims description 3
- 229960000956 coumarin Drugs 0.000 claims description 3
- 235000001671 coumarin Nutrition 0.000 claims description 3
- 238000011084 recovery Methods 0.000 claims description 3
- 241001254604 Angelica pubescens Species 0.000 claims description 2
- 244000146486 Glehnia littoralis Species 0.000 claims description 2
- 235000006662 Lansium Nutrition 0.000 claims description 2
- 241001156382 Lansium Species 0.000 claims description 2
- 244000303512 Peucedanum ostruthium Species 0.000 claims description 2
- 235000007155 Peucedanum ostruthium Nutrition 0.000 claims description 2
- 235000019084 Selinum monnieri Nutrition 0.000 claims description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical group O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 2
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 claims description 2
- 239000003463 adsorbent Substances 0.000 claims description 2
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 2
- 238000004587 chromatography analysis Methods 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 claims description 2
- 238000002425 crystallisation Methods 0.000 claims description 2
- 230000008025 crystallization Effects 0.000 claims description 2
- 235000013399 edible fruits Nutrition 0.000 claims description 2
- 229930003944 flavone Natural products 0.000 claims description 2
- 150000002213 flavones Chemical class 0.000 claims description 2
- 235000011949 flavones Nutrition 0.000 claims description 2
- 239000001530 fumaric acid Substances 0.000 claims description 2
- 239000011259 mixed solution Substances 0.000 claims description 2
- 150000007524 organic acids Chemical class 0.000 claims description 2
- 239000000741 silica gel Substances 0.000 claims description 2
- 229910002027 silica gel Inorganic materials 0.000 claims description 2
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims description 2
- 244000061176 Nicotiana tabacum Species 0.000 claims 2
- 235000004036 Glehnia littoralis Nutrition 0.000 claims 1
- 244000226566 Psoralea corylifolia Species 0.000 claims 1
- 235000005187 Taraxacum officinale ssp. officinale Nutrition 0.000 claims 1
- 230000006378 damage Effects 0.000 abstract description 10
- 241000736026 Sarcandra Species 0.000 abstract 2
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 241000208125 Nicotiana Species 0.000 description 13
- 230000036541 health Effects 0.000 description 12
- 230000000391 smoking effect Effects 0.000 description 8
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- 230000006837 decompression Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 210000004072 lung Anatomy 0.000 description 3
- 239000000779 smoke Substances 0.000 description 3
- 241000213006 Angelica dahurica Species 0.000 description 2
- FMMWHPNWAFZXNH-UHFFFAOYSA-N Benz[a]pyrene Chemical compound C1=C2C3=CC=CC=C3C=C(C=C3)C2=C2C3=CC=CC2=C1 FMMWHPNWAFZXNH-UHFFFAOYSA-N 0.000 description 2
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 2
- 244000241463 Cullen corylifolium Species 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 238000004166 bioassay Methods 0.000 description 2
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000003546 flue gas Substances 0.000 description 2
- 241000411851 herbal medicine Species 0.000 description 2
- XKLJHFLUAHKGGU-UHFFFAOYSA-N nitrous amide Chemical compound ON=N XKLJHFLUAHKGGU-UHFFFAOYSA-N 0.000 description 2
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- STGNLGBPLOVYMA-MAZDBSFSSA-N (E)-but-2-enedioic acid Chemical compound OC(=O)\C=C\C(O)=O.OC(=O)\C=C\C(O)=O STGNLGBPLOVYMA-MAZDBSFSSA-N 0.000 description 1
- GOLORTLGFDVFDW-UHFFFAOYSA-N 3-(1h-benzimidazol-2-yl)-7-(diethylamino)chromen-2-one Chemical compound C1=CC=C2NC(C3=CC4=CC=C(C=C4OC3=O)N(CC)CC)=NC2=C1 GOLORTLGFDVFDW-UHFFFAOYSA-N 0.000 description 1
- 241000510531 Angelica decursiva Species 0.000 description 1
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- 241001254606 Peucedanum praeruptorum Species 0.000 description 1
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Abstract
The present invention relates to a cigarette additive extracted from natural plants, an extractive method thereof, and the application thereof in cigarettes. The cigarette additive contains 5% to 100% of total coumarin derivative extract, 0 to 95% of sarcandra extract and 0 to 95% of dandelion extract, wherein the total coumarin derivative extract, the sarcandra extract and the dandelion extract are all extracted from natural plants. The total coumarin derivative extract can be obtained by extracting or leaching with water and organic solvent, or extracting by a percolation method and then recrystallization; or the total coumarin derivative extract can be obtained by extracting and separating with supercritical carbon dioxide after extraction, and then retreating or recrystallization; or the total coumarin derivative extract is directly extracted by supercritical carbon dioxide. The cigarette additive can be applied to cigarettes to manufacture health-care cigarettes, so that the harm of cigarettes can be significantly reduced, and the safety of cigarettes can be obviously enhanced.
Description
Technical field
The present invention relates to a kind of cigarette additives and extracting method thereof and the application in cigarette thereof.
Background technology
The harmfulness of smoking health, in the smog of cigarette, contain multiple noxious material, can cause cancer, arterial thrombotic disease, immunologic function reduces, breathing problem, increasing of disease incidences such as hypogona dism, but for long-term smoker, smoking cessation is often very difficult, external researcher generally is devoted to improve the prescription of tobacco, reduce the wherein content of tar, the researcher of China then utilizes the treasure-house of traditional traditional Chinese medicine and pharmacy, the plant or its extract or its volatile oil that add Chinese herbal medicine in the pipe tobacco, so that the smoker can suck the effective medicinal components in the Chinese herbal medicine in smoking process, thus the harm of weakening or minimizing cigarette.This is the very important approach that tobacco business reduces cigarette harm, also is that development has the new measure of China's characteristic cigarette.
Summary of the invention
First purpose of the present invention is to provide a kind of cigarette additives from extracted form natural plant, and this additive can reduce the harm of cigarette to human body, thereby improves the security of cigarette.
Second purpose of the present invention is to provide the extracting method of above-mentioned cigarette additives.
The 3rd purpose of the present invention is above-mentioned cigarette additives from extracted form natural plant is applied to cigarette.
First purpose of the present invention is achieved in that
Cigarette additives of the present invention contains the general coumarin derivative extract of 5%-100%, the Herba Pileae Scriptae extract of 0-95%, the dandelion extract of 0-95%;
The extract of described general coumarin derivative is from the Carrot family plant, legume, extract in the plant of one or more of rutaceae, the plant part of being got is complete stool or its root of plant, stem, branch, leaf, flower, fruit, seed, the Carrot family plant is as glehnia littoralis Glehnia Littoralis Fr.Schmidt ex Miq, Imperatoria ostruthium Peucedanumosthru thium (L.) Koch, RADIX PEUCEDANI Peucedanum Praeruptorum Dunn, RADIX PEUCEDANI Peucedanum decursivum Maxim, Angelica pubescens Angelica pubescensMaxim, root of Dahurain angelica Angelica dahurica (Fisch.ex Hoffm..) Benth.et Hook.f., Radix angelicae dahuricae Angelica dahurica (Fisch..ex Hoffm.) Benth.et Hook.f.Varformosana (Boiss.) shan et Yuan, cnidium monnieri Cnidium monnieri (L.) Cuss., legume such as Psoralea corylifolia Psoralea Corylifolia L., rutaceae such as anistree Calusena lansium Clausena anisata Oliv., single leaf rue Haplophyllum bungei Trautv; The main component of general coumarin derivative extract is the derivative of general coumarin, and the derivative of general coumarin is one or more the compound in following formula (1), the formula (2):
Formula (1)
R
1＝H，OH，OCH
3；
Formula (2) R
1=H, OH, OCH
3,
R
2＝H，OH，OCH
3，
Described Herba Pileae Scriptae extract is to extract from the herb of golden grain Zhu Ke plant plait coral Sarcandra glabra (Thunb.) Nakai drying and get, and its main component is general coumarin lactone, lactone, flavones and organic acid, as isofraxidin isofraxidin
Fumaric acid (Fumaric acid)
Described dandelion extract is that the dry herb by feverfew dandelion Taraxacum mongolicumHand.-Mazz. and congener thereof extracts and gets.
Second purpose of the present invention is achieved in that
The extracting method of general coumarin derivative extract has three kinds, and first kind of extracting method comprises the steps:
(1) gets one or more plant complete stool drying in Carrot family plant, legume, rutaceae and the equal platymiscium, pulverize, water or pure heating and refluxing extraction, or lixiviate, or, extract twice, each 1～24 hour with the percolation extraction, used alcohol is ethanol or methyl alcohol, or they and the formulated concentration of water are 50～100% alcoholic solution;
(2) extract is filtered, be condensed into paste after reclaiming solvent, this medicinal extract extracts with organic solvent again, the same or extraction of extracting method, used organic solvent is selected oxolane for use, hexane, benzinum, benzene, chloroform, ether, acetone, ethyl acetate, ethanol, methyl alcohol, extract reclaims solvent, as do not have crystallization and separate out, extract is handled with ammoniacal liquor or dilute alkaline soln again, washes with water to neutrality, reclaim solvent again, place about 0 ℃, separate out if any crystal, then the leaching crystal, use one or more recrystallizations of above-mentioned organic solvent again, promptly get general coumarin derivative extract; As do not have crystal and separate out, then carrying out chromatography, adsorbent is silica gel or aluminium oxide, eluant, eluent is the mixed solution of one or more different proportions of above-mentioned organic solvent, and eluent reclaims solvent, is placed with crystal about 0 ℃ and separates out, with above-mentioned method recrystallization, promptly.
The extracting method of second kind of general coumarin derivative extract comprises the steps:
(1) gets one or more plant complete stool drying in Carrot family plant, legume, rutaceae and the equal platymiscium, pulverize, water or pure heating and refluxing extraction, or lixiviate, or, extract twice, each 1～24 hour with the percolation extraction, used alcohol is ethanol or methyl alcohol, or they and the formulated concentration of water are 50～100% alcoholic solution;
(2) extract is filtered, be condensed into paste behind the recovery solvent, separate with supercritical carbon dioxide extracting again, obtain crystal and re-refine or be recrystallized, promptly.
The extracting method of the third general coumarin derivative extract comprise the steps: to get in Carrot family plant, legume, rutaceae and the equal platymiscium one or more plant drying, be ground into meal, extract with supercritical carbon dioxide, obtain crystal and re-refine or be recrystallized, promptly.
Herba Pileae Scriptae extract and dandelion extract can adopt following three kinds of methods to extract:
(1) Chloranthus glaber or dandelion plant herb drying, pulverizing, water or pure heating and refluxing extraction, or lixiviate, or percolation extracts, and extracts secondary, each 1～24 hour, used alcohol was the alcoholic solution of ethanol or methyl alcohol or 50～100% concentration that are made into water; Extract is filtered, and decompression or normal pressure reclaim solvent, and extract is condensed into paste, this paste water, alcohol or acetone, ethyl acetate repeated treatments, reclaim solvent, are concentrated into paste promptly.
(2) Chloranthus glaber or dandelion plant herb drying, be ground into meal, extract with supercritical carbon dioxide.
(3) in the aforementioned first method through the paste of water or alcohol extracting gained, promptly with supercritical carbon dioxide treatment.
Cigarette additives of the present invention is applied in the cigarette, be to be cigarette additives and concentration that the ethanolic solution of 50%-95% is mixed with cigarette additive and is added into and makes health cigarette in the cigarette, the adding proportion of this additive is that the 0.01%-10% of pipe tobacco weight is better.
Contain multiple beneficial such as general coumarin derivative, isofraxidin, fumaric acid in the active material of health in general coumarin derivative extract, Herba Pileae Scriptae extract, the dandelion extract, have physiology and pharmacological effect widely, can fully coordinate with pipe tobacco aspect color, smell and taste, wherein the easy gasification of active ingredient enters respiratory tract with main flume.Utilize the HPLC method that the general coumarin derivative in the cigarette additives of the present invention has been carried out assay determination, the result shows: the general coumarin derivative content is the cigarette that 18.55 μ g/ prop up, the content of general coumarin derivative is that 5.22 μ g/ prop up in its flue gas, and the rate of transform is more than 28.14%.Further experiment shows: the existence of general coumarin derivative is also arranged in the serum of chronic suction experiment cigarette mouse, illustrate that the main component general coumarin derivative of cigarette additives of the present invention can be absorbed in the blood by oral cavity etc.
The applicant has carried out comprehensive test from aspects such as active ingredient transfer, flue gas analysis, security biological assessment and effect detections to cigarette additives of the present invention, provides direct, objective and ample evidence cigarette additives of the present invention is applied to can reduce cigarette harm in the cigarette.
Result of study shows: cigarette additives of the present invention has an obvious blocking effect to volatile nitrosamine and the distinctive nitrosamine of tobacco are synthetic; Mutagenicity to benzo [a] pyrene has significant inhibitory effect.Cigarette additive is being higher than when using 1000 times of concentration, and toxic and side effect is not all observed in animal and cell in vitro test, shows cigarette additives safety non-toxic of the present invention.
On this basis, the applicant studies the effect that cigarette additives of the present invention is applied to can to reduce in the cigarette cigarette harm, the cigarette that has added additive of the present invention has been carried out the smoke chemistry analysis and the biological assessment of system.The result shows: compare with the contrast cigarette that does not add additive of the present invention, can significantly alleviate bad reactions such as caused by smoking cough, asthma, stasis of blood phlegm, obviously reduce the incidence of disease of breathing problem; The free radical level reduces more than 20%; Make the time and the deadly smoking capacity of mouse and rat smoking acute death increase about 60% and 30% respectively; Particularly when smoking control group cigarette treated animal was all dead, additive cigarette treated animal survival rate was up to more than 85%; The acute toxic reaction of mouse obviously alleviates, and the prolonged survival period that shows animal is more than 1 times, and deadly smoking capacity significantly increases; Lung tissue pathology observed result shows: be all the acute or chronic lethal animal of smoking, experiment cigarette group lung tissue degree of injury obviously alleviates, and shows that lymphocytic infiltration, alveolus wall thicken, hemorrhage, tracheaectasy, epithelium attenuation, lung sac bubble and manage multinomial pathology damage index such as all oedema all significantly better than contrast cigarette group; Significantly sister chromosome exchanges and the marrow microkernel incidence due to the inhibition cigarette condensation product; Make that the dosage of SHE and the death of BEAS-2B cell half is increased to 1.7 and 4 times respectively due to the cigarette smoke, the level of bringing out the ROS that cell produces obviously reduces, and shows H in cell
2O
2And O
2 -Level reduces for work; With with quality contrast cigarette relatively, cytotoxicity has reduced 2-4 doubly, the cellular oxidation that cigarette smoke brings out, cell membrane damage, chromogene sudden change and cell transformation effect obviously reduce.
Above result of study shows: the cigarette additives of using the present inventor can significantly reduce the harm of cigarette, the security that obviously improves cigarette.
The invention will be further described below in conjunction with embodiment.
The specific embodiment
Embodiment one:
The preparation of general coumarin derivative extract:
Get the Psoralea corylifolia seed, drying is ground into meal, mix wetly with 60% ethanol, well-established law is packed in the diacolation cylinder, and lixiviate is diacolation after 12 hours, collect the percolate of 10 times of amounts, decompression recycling ethanol leaves standstill, the collecting precipitation thing, sediment is handled with methyl alcohol, left standstill, promptly have crystal to separate out, collect, being recrystallized routinely with methyl alcohol promptly gets general coumarin derivative extract.
Getting above gained general coumarin derivative extract 1400 μ g, to dissolve in 1ml concentration as cigarette additives be 90% ethanolic solution, is mixed with the cigarette additive that cigarette additives concentration is 1400 μ g/ml.Cigarette additive is added in the pipe tobacco with table perfume (or spice), and the addition of cigarette additives is 0.2% of a pipe tobacco weight, and cigarette process is made a health care cigarette routinely, the heavy 0.7g of Linear Leaf Speedwell cigarette, and every health care cigarette contains additive 1400 μ g.
Embodiment two:
The preparation of general coumarin derivative extract:
(1) get the seed of Carrot family plant cnidium monnieri, drying is pulverized, and extracts secondary with 95% alcohol reflux earlier, and each 2 hours, extract merged and filters, and is condensed into paste behind the recovery solvent;
(2) paste is separated with supercritical carbon dioxide extracting, obtain crystal and re-refine, promptly get general coumarin derivative extract.
The general coumarin derivative extract 70 μ g that get gained make cigarette additive by the method for embodiment one and are added in the pipe tobacco, make a health care cigarette, the heavy 0.7g of Linear Leaf Speedwell cigarette, adopting embodiment one cigarette additive 90% ethanolic solution to change concentration into is 70% ethanolic solution, and the adding proportion of cigarette additives is 0.01% of a pipe tobacco weight.
Embodiment three:
The preparation of general coumarin derivative extract: get the root of the Carrot family plant root of Dahurain angelica, drying is ground into meal, extracts with supercritical carbon dioxide, leaves standstill, and gets its precipitate and gets general coumarin derivative extract, re-refines promptly.
The preparation of Herba Pileae Scriptae extract and dandelion extract: Chloranthus glaber or dandelion plant herb drying, pulverizing, elder generation's water heating and refluxing extraction 2 times, each 2 hours, merging extract filters, decompression is condensed into rare paste, this paste Ethanol Treatment, handles with ethyl acetate again, reclaim solvent, be concentrated into paste and promptly get Herba Pileae Scriptae extract or dandelion extract.
Get above gained general coumarin derivative extract 800 μ g, Herba Pileae Scriptae extract 2000 μ g, dandelion extract 5200 μ g, method by embodiment one is added in the pipe tobacco, make a health care cigarette, the weight percentage of cigarette additives is 1.14% in this health care cigarette.
Embodiment four:
The preparation of Herba Pileae Scriptae extract: get Chloranthus glaber plant herb, dry, be ground into meal, extract with supercritical carbon dioxide, promptly get Herba Pileae Scriptae extract.
The preparation of general coumarin derivative extract is with embodiment one.
Get above-mentioned gained Herba Pileae Scriptae extract 66.5mg and general coumarin derivative extract 3.5mg, method by embodiment one is made cigarette additives, be added into then in the pipe tobacco, make a health care cigarette, the weight percentage of cigarette additives is 10% in this health care cigarette.
Embodiment five:
The preparation of dandelion extract: preceding step is with embodiment three, and the paste of water, alcohol extract gained promptly gets dandelion extract with supercritical carbon dioxide treatment.
Get above-mentioned gained dandelion extract 6.65mg and general coumarin derivative extract 0.35mg, method by embodiment one is made cigarette additives, be added into then in the pipe tobacco, make a health care cigarette, the weight percentage of cigarette additives is 1% in this health care cigarette.
Dandelion extract also can adopt the extracting method of Herba Pileae Scriptae extract among the embodiment four and gets in the present embodiment, and Herba Pileae Scriptae extract can adopt the extracting method of dandelion extract in the present embodiment and gets among the embodiment four.
Claims (7)
1. the cigarette additives from extracted form natural plant is characterized in that: contain the general coumarin derivative extract of 5%-100%, the Herba Pileae Scriptae extract of 0-95%, the dandelion extract of 0-95%;
The extract of described general coumarin derivative is to extract from one or more plant of Carrot family plant, legume, rutaceae, the plant part of being got is the complete stool of plant or its root, stem, branch, leaf, flower, fruit, seed, its main component is the general coumarin derivative, and the general coumarin derivative is one or more the compound in formula (1), the formula (2):
R
1＝H，OH，OCH
3；
Formula (2)
Described Herba Pileae Scriptae extract is to extract from the herb of Chloranthus glaber drying and get, and its main component is general coumarin lactone, flavones and organic acid, comprises isofraxidin, fumaric acid;
Described dandelion extract is that the dry herb by dandelion and congener thereof extracts and gets.
2. the cigarette additives from extracted form natural plant according to claim 1, it is characterized in that: described Carrot family plant comprises glehnia littoralis, Imperatoria ostruthium, RADIX PEUCEDANI, RADIX PEUCEDANI, Angelica pubescens, the root of Dahurain angelica, Radix angelicae dahuricae, cnidium monnieri, described legume comprises Psoralea corylifolia, described rutaceae comprises anistree Calusena lansium, single leaf rue, and congener.
3. the extracting method of the described cigarette additives of claim 1 is characterized in that the extraction step of described general coumarin derivative extract is as follows:
(1) gets one or more plant drying in Carrot family plant, legume, rutaceae and the equal platymiscium, pulverize, water or pure heating and refluxing extraction, or lixiviate, or, extract twice, each 1～24 hour with the percolation extraction, used alcohol is ethanol or methyl alcohol, or they and the formulated concentration of water are 50～100% alcoholic solution;
(2) extract is filtered, be condensed into paste after reclaiming solvent, this medicinal extract extracts with organic solvent again, the same or extraction of extracting method, used organic solvent is selected oxolane for use, hexane, benzinum, benzene, chloroform, ether, acetone, ethyl acetate, ethanol, methyl alcohol, extract reclaims solvent, as do not have crystallization and separate out, extract is handled with ammoniacal liquor or dilute alkaline soln again, washes with water to neutrality, reclaim solvent again, place about 0 ℃, separate out if any crystal, then the leaching crystal, use one or more recrystallizations of above-mentioned organic solvent again, promptly get general coumarin derivative extract; As do not have crystal and separate out, then carrying out chromatography, adsorbent is silica gel or aluminium oxide, eluant, eluent is the mixed solution of one or more different proportions of above-mentioned organic solvent, and eluent reclaims solvent, is placed with crystal about 0 ℃ and separates out, with above-mentioned method recrystallization, promptly.
4. the extracting method of the described cigarette additives of claim 1 is characterized in that the extraction step of described general coumarin derivative extract is as follows:
(1) gets one or more plant drying in Carrot family plant, legume, rutaceae and the equal platymiscium, pulverize, water or pure heating and refluxing extraction, or lixiviate, or, extract twice, each 1～24 hour with the percolation extraction, used alcohol is ethanol or methyl alcohol, or they and the formulated concentration of water are 50～100% alcoholic solution;
(2) extract is filtered, be condensed into paste behind the recovery solvent, separate with supercritical carbon dioxide extracting again, obtain crystal and re-refine or be recrystallized, promptly.
5. the extracting method of the described cigarette additives of claim 1, the extraction step that it is characterized in that described general coumarin derivative extract is as follows: get in Carrot family plant, legume, rutaceae and the equal platymiscium one or more plant drying, be ground into meal, extract with supercritical carbon dioxide, obtain crystal and re-refine or be recrystallized, promptly.
6. the application of the described cigarette additives of claim 1 in cigarette.
7. the application of cigarette additives according to claim 6 in cigarette is characterized in that: the 0.01%-10% ratio of this additive in pipe tobacco weight is added in the pipe tobacco.
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| CN 03140229 CN1225998C (en) | 2003-08-19 | 2003-08-19 | Cigarette additive extracted from natural plant, and extracting method and use in cigarette thereof |
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| CN 03140229 CN1225998C (en) | 2003-08-19 | 2003-08-19 | Cigarette additive extracted from natural plant, and extracting method and use in cigarette thereof |
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| CN1225998C true CN1225998C (en) | 2005-11-09 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102754911A (en) * | 2012-07-02 | 2012-10-31 | 广西中烟工业有限责任公司 | Harm reducing natural plant additive for cigarettes, and preparation method and application thereof |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090028898A1 (en) * | 2005-03-14 | 2009-01-29 | Xixian Qiu | Instant Additive Solution for the Filter of Cigarette, Its Production Method and Use |
| KR100833652B1 (en) * | 2006-12-28 | 2008-05-29 | 한국화학연구원 | Composition for preventing or treating a neurodegenerative disease comprising an extract from a seed of psoralea corylifolia inhibiting the activity of bace-1 or active ingredients thereof |
| CN101669674A (en) * | 2008-09-10 | 2010-03-17 | 江西中烟工业公司 | Pilocarpus plant extract for reducing smoke dry sensation of cigarettes and preparation method thereof |
| CN101863896B (en) * | 2010-06-22 | 2012-07-18 | 浙江大学 | Composite extraction method for extracting coumarin from radix angelicae dahuricae |
| CN102090712B (en) * | 2010-12-29 | 2013-01-23 | 华宝食用香精香料（上海）有限公司 | Method for preparing coarse extract of peanut shell total flavones and application thereof in cigarettes |
| CN104905405B (en) * | 2015-06-17 | 2018-05-25 | 湖北中烟工业有限责任公司 | The cigarette of the essence containing plant flavone mouth stick preparation method and application |
| CN112515237B (en) * | 2020-12-04 | 2021-11-23 | 新火智造(深圳)有限公司 | Manufacturing method of environment-friendly wrapping-free heating non-combustion naked cigarette bomb |
| CN112471590B (en) * | 2020-12-04 | 2021-11-02 | 新火智造(深圳)有限公司 | Environment-friendly wrapping-free heating non-combustion naked cigarette bomb |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102754911A (en) * | 2012-07-02 | 2012-10-31 | 广西中烟工业有限责任公司 | Harm reducing natural plant additive for cigarettes, and preparation method and application thereof |
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