CN101085096B - Method for extracting cordate houttuynia - Google Patents
Method for extracting cordate houttuynia Download PDFInfo
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- CN101085096B CN101085096B CN2007100234396A CN200710023439A CN101085096B CN 101085096 B CN101085096 B CN 101085096B CN 2007100234396 A CN2007100234396 A CN 2007100234396A CN 200710023439 A CN200710023439 A CN 200710023439A CN 101085096 B CN101085096 B CN 101085096B
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- herba houttuyniae
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- extractum
- ethyl acetate
- petroleum ether
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Abstract
The invention discloses a process for extracting houttuynia cordata which comprises an alcohol extraction step, a water extraction step and a purification step. Compared with the prior art, the invention is characterized in that the combination extraction method can be employed to effectively extract the medicinal portion of the houttuynia cordata to obtain cordate houttuynia injection, desinfactant detergent and health vinegar.
Description
Technical field:
The invention belongs to this technical field of extracting method of Chinese medicine, belong to this technical field of extracting method of Herba Houttuyniae especially.
Background technology:
Herba Houttuyniae claims Herba Houttuyniae, hog snout hole, Herba rutae, fish scale grass again, and its property of medicine suffering is slightly cold.Return lung meridian, have heat clearing away, detoxifcation, dampness removing, detumescence.Be used for pulmonary abscess, phlegm-heat cough, oedema due to nephritis, leucorrhea, urinary tract infection, the treatment of carbuncle furuncle.Its herb contains volatile oil, main component is a houttuynine sodium bisulfite in the oil, methylnonanone, laurel fat alkene, sad and lauryl aldehyde etc., have special foul smell, houttuynine sodium bisulfite is the main antimicrobial component of Herba Houttuyniae, to micrococcus catarrhalis, hemophilus influenza, streptococcus pneumoniae, staphylococcus aureuses etc. have obvious inhibitory action, contain quercitrin in the leaf, spica and fruit ear contain Isoquercitrin, and quercitrin has antiviral and diuresis, and Herba Houttuyniae can also the enhancing human body immunity function in addition, the leukocyte increasing phagocytic activity, has analgesia, cough-relieving, hemostasis promotes tissue regeneration, the expansion blood capillary, the effect of aspects such as blood flow increasing.
Herba Houttuyniae extracting method commonly used distills the liquid after the distillation once more for the water that earlier Herba Houttuyniae (dry product) is added its 50 times of volumes carries out steam distillation, and the Herba Houttuyniae extract that this kind method is prepared can not extract the effective ingredient in the Herba Houttuyniae fully.
Summary of the invention:
Technical problem to be solved by this invention provides a kind of new Herba Houttuyniae extracting method.
The technical scheme of technical solution problem of the present invention is: a kind of extracting method of Herba Houttuyniae, comprise the alcohol extraction operation, and water is carried operation, purification process:
Described alcohol extraction operation is for being ethanol extraction 0.5-5 hour of 50-80% with volumetric concentration with the dry product Herba Houttuyniae under room temperature, stirring condition, and Herba Houttuyniae and alcoholic acid by weight/volume are 1: 10-50; Filter, it is 1.05-1.1 gram/cm that filtrate is condensed into density
2Extractum;
Described water carry operation for the filtering residue of alcohol extraction operation under 40-50 ℃ condition, with water extraction 1-5 hour, the weight ratio of filtering residue and water was 1: 10-20, filtration, it is 1.05-1.1 gram/cm that filtrate is condensed into density
2Extractum;
Described purification process is carried the prepared extractum of operation for the extractum that the alcohol extraction operation is prepared with water and is separated with silicagel column, and silica gel and applied sample amount weight ratio are not less than 0.5; Eluent is the mixed solvent of petroleum ether and ethyl acetate, and the two blended volume ratio is petroleum ether: ethyl acetate=10-5: 1.
Put forward the degree of cordate houttuynia total flavones in the operation in order to improve water, carry in the operation, can also add cellulase and the protease of the 1-1.5% that accounts for the filtering residue gross weight at water.
Described cellulase is Trichoderma spp. CGMCC 3.3008.
In separation circuit, preferred silica gel and applied sample amount weight ratio are 0.8-1.3: 1; Eluent is petroleum ether, ethyl acetate: the mixed solvent of acetone, the blended volume ratio of three is petroleum ether: ethyl acetate=10-5: 1: 0.5.
The present invention compared with prior art by using the mixed extraction method, can effectively extract the medicinal part in the Herba Houttuyniae, and the Herba Houttuyniae extract prepared with the present invention can be used for Herba Houttuyniae injectio, desinfactant detergent, health promoting vinegar.
The specific embodiment:
The present invention will be described in detail below in conjunction with embodiment.
The detection method of total flavones of the present invention is that reference substance is measured content of total flavone in the Herba Houttuyniae with the rutin for foundation, add aluminium ion reagent, the suitable pH value of control simultaneously makes chromocor compound and aluminum salt formation complex, can obtain the stable characteristics absworption peak at visible region.
Embodiment 1:
1, alcohol extraction operation: is 50% 5 ton ethanol extractions 5 hour with volumetric concentration with dry product Herba Houttuyniae 100kg under room temperature, stirring condition, and it is 1.05-1.1 gram/cm that filtrate is condensed into density
2Extractum, reclaim ethanol;
2, water is put forward operation: the 100kg filtering residue of getting the alcohol extraction operation adds 2 tons in water under 40 ℃ condition, extracts 5 hours, filters, and it is 1.05-1.1 gram/cm that filtrate is condensed into density
2Extractum;
3, purification process, extractum that the alcohol extraction operation is prepared and water are put forward the prepared extractum mix homogeneously of operation, silica gel and fluid extract weight ratio 1: 1; Eluent is the mixed solvent of petroleum ether and ethyl acetate, and the two blended volume ratio is a petroleum ether: ethyl acetate=10: 1, eluent flow rate are 3ml/min, collect the total flavones in the Herba Houttuyniae, get final product.
The total flavones of embodiment 1 counts 6.5% with the dry product Herba Houttuyniae
Embodiment 2:
1, alcohol extraction operation: is 80% 1 ton ethanol extraction 5 hour with volumetric concentration with dry product Herba Houttuyniae 100kg under room temperature, stirring condition, and it is 1.05-1.1 gram/cm that filtrate is condensed into density
2Extractum, reclaim ethanol;
2, water is put forward operation: the 100kg filtering residue of getting the alcohol extraction operation adds 1 ton in water under 50 ℃ condition, extracts 0.5 hour, filters, and it is 1.05-1.1 gram/cm that filtrate is condensed into density
2Extractum;
3, purification process, extractum that the alcohol extraction operation is prepared and water are put forward the prepared extractum mix homogeneously of operation, silica gel and fluid extract weight ratio 1: 1.2; Eluent is the mixed solvent of petroleum ether and ethyl acetate, and the two blended volume ratio is a petroleum ether: ethyl acetate=5: 1, eluent flow rate are 3ml/min, collect the total flavones in the Herba Houttuyniae, get final product.
The total flavones of embodiment 2 counts 6.65% with the dry product Herba Houttuyniae
Embodiment 3:
Add 1% the Trichoderma spp. CGMCC 3.3008 and protease that accounts for the filtering residue gross weight except that carrying at water in the operation, all the other are identical with embodiment 2.
The total flavones of embodiment 3 counts 6.8% with the dry product Herba Houttuyniae
Embodiment 4:
Remove in separation circuit, eluent is petroleum ether and ethyl acetate: the mixed solvent of acetone, the blended volume ratio of three is a petroleum ether: ethyl acetate: acetone=outside 8: 1: 0.5, all the other are identical with embodiment 3.
The total flavones of embodiment 4 counts 6.75% with the dry product Herba Houttuyniae.
Claims (4)
1. the extracting method of a Herba Houttuyniae comprises the alcohol extraction operation, and water is carried operation, purification process:
Described alcohol extraction operation is for being ethanol extraction 0.5-5 hour of 50-80% with volumetric concentration with the dry product Herba Houttuyniae under room temperature, stirring condition, and Herba Houttuyniae and alcoholic acid by weight/volume are 1: 10-50; Filter, it is 1.05-1.1 gram/cm that filtrate is condensed into density
2Extractum;
Described water carry operation for the filtering residue of alcohol extraction operation under 40-50 ℃ condition, with water extraction 1-5 hour, the weight ratio of filtering residue and water was 1: 10-20, filtration, it is 1.05-1.1 gram/cm that filtrate is condensed into density
2Extractum;
Described purification process is carried the prepared extractum of operation for the extractum that the alcohol extraction operation is prepared with water and is separated with silicagel column, and silica gel and applied sample amount weight ratio are not less than 0.5; Eluent is the mixed solvent of petroleum ether and ethyl acetate, and the two blended volume ratio is petroleum ether: ethyl acetate=10-5: 1.
2. the extracting method of a kind of Herba Houttuyniae according to claim 1 is characterized in that: carry in the operation at water, add cellulase and the protease of the 1-1.5% that accounts for the filtering residue gross weight.
3. the extracting method of a kind of Herba Houttuyniae according to claim 2, it is characterized in that: described cellulase is Trichoderma spp. CGMCC 3.3008.
4. the extracting method of a kind of Herba Houttuyniae according to claim 1, it is characterized in that: in purification process, silica gel and applied sample amount weight ratio are 0.8-1.3: 1; Eluent replaces with the mixed solvent of petroleum ether, ethyl acetate, acetone, and the blended volume ratio of three is a petroleum ether: ethyl acetate: acetone=10-5: 1: 0.5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100234396A CN101085096B (en) | 2007-06-04 | 2007-06-04 | Method for extracting cordate houttuynia |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100234396A CN101085096B (en) | 2007-06-04 | 2007-06-04 | Method for extracting cordate houttuynia |
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| Publication Number | Publication Date |
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| CN101085096A CN101085096A (en) | 2007-12-12 |
| CN101085096B true CN101085096B (en) | 2011-04-13 |
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| CN2007100234396A Expired - Fee Related CN101085096B (en) | 2007-06-04 | 2007-06-04 | Method for extracting cordate houttuynia |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103181973B (en) * | 2011-12-31 | 2016-03-02 | 新昌县冠阳技术开发有限公司 | A kind of manufacture method of Herba Houttuyniae oil |
| CN103055006B (en) * | 2013-02-05 | 2014-04-23 | 河南省医药科学研究院 | Effective part of Houttuynia cordata as well as extracting method and application thereof |
| CN105802275A (en) * | 2016-03-25 | 2016-07-27 | 赤峰昭乌达羊绒制品有限责任公司 | Preparation method and application of herba houttuyniae natural dye |
| CN106074821A (en) * | 2016-07-27 | 2016-11-09 | 南京正宽医药科技有限公司 | The process of preparing Chinese medicine extracting method of a kind of Herba Houttuyniae decoction pieces and Herba Houttuyniae compositions |
| CN110538246A (en) * | 2019-08-26 | 2019-12-06 | 苏州市天灵中药饮片有限公司 | High-efficiency extraction process of compound traditional Chinese medicine |
| CN111528455A (en) * | 2020-06-03 | 2020-08-14 | 安徽省味香居食品有限公司 | Production process of instant stewed beef with hericium erinaceus |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1480179A (en) * | 2003-07-21 | 2004-03-10 | 洪彰福 | Preparation of houttuynia for treating peptic ulcer as well as acute and chronic gastritis |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1480179A (en) * | 2003-07-21 | 2004-03-10 | 洪彰福 | Preparation of houttuynia for treating peptic ulcer as well as acute and chronic gastritis |
Non-Patent Citations (2)
| Title |
|---|
| 戴伟锋等.鱼腥草中总黄酮提取工艺考察.云南化工33 1.2006,33(1),11-14. |
| 戴伟锋等.鱼腥草中总黄酮提取工艺考察.云南化工33 1.2006,33(1),11-14. * |
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