CN1215175C - 蛋白质生产方法 - Google Patents
蛋白质生产方法 Download PDFInfo
- Publication number
- CN1215175C CN1215175C CNB98118992XA CN98118992A CN1215175C CN 1215175 C CN1215175 C CN 1215175C CN B98118992X A CNB98118992X A CN B98118992XA CN 98118992 A CN98118992 A CN 98118992A CN 1215175 C CN1215175 C CN 1215175C
- Authority
- CN
- China
- Prior art keywords
- enzyme
- yeast
- glucose
- carbon source
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000004519 manufacturing process Methods 0.000 title abstract description 10
- 230000014616 translation Effects 0.000 title 1
- 238000000034 method Methods 0.000 claims abstract description 38
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 25
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 25
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 21
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 20
- 238000012258 culturing Methods 0.000 claims abstract description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 30
- 239000008103 glucose Substances 0.000 claims description 28
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 27
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 21
- 102000004190 Enzymes Human genes 0.000 claims description 18
- 108090000790 Enzymes Proteins 0.000 claims description 18
- 229940088598 enzyme Drugs 0.000 claims description 18
- 108010011619 6-Phytase Proteins 0.000 claims description 15
- 229940085127 phytase Drugs 0.000 claims description 13
- 108010025188 Alcohol oxidase Proteins 0.000 claims description 8
- 241000235648 Pichia Species 0.000 claims description 8
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 7
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 6
- 239000011159 matrix material Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 229910052760 oxygen Inorganic materials 0.000 claims description 6
- 239000001301 oxygen Substances 0.000 claims description 6
- 241000235058 Komagataella pastoris Species 0.000 claims description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 5
- 229930006000 Sucrose Natural products 0.000 claims description 5
- 239000005720 sucrose Substances 0.000 claims description 5
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims description 4
- 108010059892 Cellulase Proteins 0.000 claims description 4
- 229920002472 Starch Polymers 0.000 claims description 4
- 229940106157 cellulase Drugs 0.000 claims description 4
- 239000003262 industrial enzyme Substances 0.000 claims description 4
- 239000008107 starch Substances 0.000 claims description 4
- 235000019698 starch Nutrition 0.000 claims description 4
- 229930091371 Fructose Natural products 0.000 claims description 3
- 239000005715 Fructose Substances 0.000 claims description 3
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 3
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 2
- 239000004382 Amylase Substances 0.000 claims description 2
- 108010065511 Amylases Proteins 0.000 claims description 2
- 102000013142 Amylases Human genes 0.000 claims description 2
- 102000016938 Catalase Human genes 0.000 claims description 2
- 108010053835 Catalase Proteins 0.000 claims description 2
- 102000029816 Collagenase Human genes 0.000 claims description 2
- 108060005980 Collagenase Proteins 0.000 claims description 2
- 229920002527 Glycogen Polymers 0.000 claims description 2
- 102000004882 Lipase Human genes 0.000 claims description 2
- 108090001060 Lipase Proteins 0.000 claims description 2
- 239000004367 Lipase Substances 0.000 claims description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 2
- 102000035195 Peptidases Human genes 0.000 claims description 2
- 108091005804 Peptidases Proteins 0.000 claims description 2
- 102000003992 Peroxidases Human genes 0.000 claims description 2
- 108010059820 Polygalacturonase Proteins 0.000 claims description 2
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical group Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 claims description 2
- 235000019418 amylase Nutrition 0.000 claims description 2
- 108010051210 beta-Fructofuranosidase Proteins 0.000 claims description 2
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 claims description 2
- 229920002678 cellulose Polymers 0.000 claims description 2
- 239000001913 cellulose Substances 0.000 claims description 2
- 229960002424 collagenase Drugs 0.000 claims description 2
- 239000008121 dextrose Substances 0.000 claims description 2
- RXKJFZQQPQGTFL-UHFFFAOYSA-N dihydroxyacetone Chemical compound OCC(=O)CO RXKJFZQQPQGTFL-UHFFFAOYSA-N 0.000 claims description 2
- 150000002016 disaccharides Chemical class 0.000 claims description 2
- 235000021433 fructose syrup Nutrition 0.000 claims description 2
- 235000019420 glucose oxidase Nutrition 0.000 claims description 2
- 150000004676 glycans Chemical class 0.000 claims description 2
- 229940096919 glycogen Drugs 0.000 claims description 2
- 235000011073 invertase Nutrition 0.000 claims description 2
- 235000019421 lipase Nutrition 0.000 claims description 2
- 235000013379 molasses Nutrition 0.000 claims description 2
- 108010001078 naringinase Proteins 0.000 claims description 2
- 229920001542 oligosaccharide Polymers 0.000 claims description 2
- 150000002482 oligosaccharides Chemical class 0.000 claims description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 2
- 229920001282 polysaccharide Polymers 0.000 claims description 2
- 239000005017 polysaccharide Substances 0.000 claims description 2
- 108090000698 Formate Dehydrogenases Proteins 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 235000001727 glucose Nutrition 0.000 claims 1
- 230000037353 metabolic pathway Effects 0.000 claims 1
- 210000003527 eukaryotic cell Anatomy 0.000 abstract description 2
- 230000001718 repressive effect Effects 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 54
- 235000011187 glycerol Nutrition 0.000 description 27
- 230000001580 bacterial effect Effects 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 7
- 239000002054 inoculum Substances 0.000 description 7
- 241001225321 Aspergillus fumigatus Species 0.000 description 5
- 229940091771 aspergillus fumigatus Drugs 0.000 description 5
- 230000014509 gene expression Effects 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- 229910019931 (NH4)2Fe(SO4)2 Inorganic materials 0.000 description 4
- 101100226950 Pichia angusta FMDH gene Proteins 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 229930003756 Vitamin B7 Natural products 0.000 description 4
- 230000003405 preventing effect Effects 0.000 description 4
- 235000013343 vitamin Nutrition 0.000 description 4
- 229930003231 vitamin Natural products 0.000 description 4
- 239000011782 vitamin Substances 0.000 description 4
- 229940088594 vitamin Drugs 0.000 description 4
- 239000011735 vitamin B7 Substances 0.000 description 4
- 235000011912 vitamin B7 Nutrition 0.000 description 4
- 150000003722 vitamin derivatives Chemical class 0.000 description 4
- 238000012366 Fed-batch cultivation Methods 0.000 description 3
- 241000080590 Niso Species 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 230000002503 metabolic effect Effects 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 238000009010 Bradford assay Methods 0.000 description 2
- 108010020056 Hydrogenase Proteins 0.000 description 2
- 230000003698 anagen phase Effects 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 238000004445 quantitative analysis Methods 0.000 description 2
- 238000012807 shake-flask culturing Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 101710093617 Dihydroxyacetone synthase Proteins 0.000 description 1
- 241000320412 Ogataea angusta Species 0.000 description 1
- 102000012607 Thrombomodulin Human genes 0.000 description 1
- 108010079274 Thrombomodulin Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 239000013530 defoamer Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229940006607 hirudin Drugs 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- PVFDPMYXCZLHKY-MLLWLMKGSA-M sodium [(1R,2R,4aR,8aS)-2-hydroxy-5-[(2E)-2-[(4S)-4-hydroxy-2-oxooxolan-3-ylidene]ethyl]-1,4a,6-trimethyl-2,3,4,7,8,8a-hexahydronaphthalen-1-yl]methyl sulfate Chemical compound [Na+].C([C@@H]1[C@](C)(COS([O-])(=O)=O)[C@H](O)CC[C@]11C)CC(C)=C1C\C=C1/[C@H](O)COC1=O PVFDPMYXCZLHKY-MLLWLMKGSA-M 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000013595 supernatant sample Substances 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP97117021.2 | 1997-10-01 | ||
| EP97117021 | 1997-10-01 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1218112A CN1218112A (zh) | 1999-06-02 |
| CN1215175C true CN1215175C (zh) | 2005-08-17 |
Family
ID=8227420
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB98118992XA Expired - Lifetime CN1215175C (zh) | 1997-10-01 | 1998-09-30 | 蛋白质生产方法 |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US6204012B1 (enExample) |
| EP (2) | EP0911416B1 (enExample) |
| JP (1) | JP4162774B2 (enExample) |
| KR (1) | KR100572426B1 (enExample) |
| CN (1) | CN1215175C (enExample) |
| AU (1) | AU748187B2 (enExample) |
| BR (1) | BR9803807B1 (enExample) |
| CA (1) | CA2249061C (enExample) |
| DE (1) | DE69834533T2 (enExample) |
| DK (1) | DK0911416T3 (enExample) |
| ES (1) | ES2264181T3 (enExample) |
| IN (1) | IN188151B (enExample) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6451572B1 (en) | 1998-06-25 | 2002-09-17 | Cornell Research Foundation, Inc. | Overexpression of phytase genes in yeast systems |
| ATE301198T1 (de) * | 1999-03-31 | 2005-08-15 | Cornell Res Foundation Inc | Phospahatasen mit verbesserter phytase-aktivität |
| US6841370B1 (en) | 1999-11-18 | 2005-01-11 | Cornell Research Foundation, Inc. | Site-directed mutagenesis of Escherichia coli phytase |
| ES2597503T3 (es) * | 2001-10-31 | 2017-01-19 | Huvepharma Eood | Alimento para animales que contiene fitasa y método |
| KR100429331B1 (ko) * | 2002-01-29 | 2004-04-29 | 재단법인 목암생명공학연구소 | 재조합 효모를 이용한 에스에이치엘케이엔-1 단백질의대량생산방법 |
| PL376381A1 (en) * | 2002-07-15 | 2005-12-27 | Immunex Corporation | Methods and media for controlling sialylation of proteins produced by mammalian cells |
| DE60335497D1 (de) | 2002-09-13 | 2011-02-03 | Cornell Res Foundation Inc | Ergillus-phytasen |
| CA2534352A1 (en) * | 2003-08-08 | 2005-02-17 | Arriva Pharmaceuticals, Inc. | Methods of protein production in yeast |
| PL1684719T3 (pl) * | 2003-11-14 | 2012-11-30 | Baxalta Inc | Kompozycje alfa 1-antytrypsyny i sposoby leczenia z zastosowaniem takich kompozycji |
| WO2006036678A2 (en) * | 2004-09-22 | 2006-04-06 | Bioworks, Inc. | Transgenic strains of trichoderma and their use in biocontrol |
| US20070105768A1 (en) * | 2004-11-10 | 2007-05-10 | Rajiv Nayar | Dry recombinant human alpha 1-antitrypsin formulation |
| KR101283288B1 (ko) | 2005-03-24 | 2013-07-11 | 디에스엠 아이피 어셋츠 비.브이. | 유용한 화합물의 미생물적 제조 방법 |
| US7919297B2 (en) * | 2006-02-21 | 2011-04-05 | Cornell Research Foundation, Inc. | Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase |
| AU2007244753B2 (en) * | 2006-04-28 | 2012-05-17 | C5-6 Technologies, Inc. | Thermostable cellulase and methods of use |
| WO2008017066A2 (en) * | 2006-08-03 | 2008-02-07 | Cornell Research Foundation, Inc. | Phytases with improved thermal stability |
| EP2106447B1 (en) | 2007-01-26 | 2013-04-17 | Novozymes A/S | Method for methanol independent induction from methanol inducible promoters in pichia |
| US8192734B2 (en) | 2007-07-09 | 2012-06-05 | Cornell University | Compositions and methods for bone strengthening |
| CN103060216B (zh) * | 2008-05-06 | 2016-07-06 | 阿彻丹尼尔斯米德兰德公司 | 通过淀粉和木聚糖的降解酶的表达开发能进行淀粉和木聚糖的醇发酵的耐热酵母多形汉逊酵母菌株 |
| GB201308828D0 (en) | 2013-03-12 | 2013-07-03 | Verenium Corp | Phytase |
| GB201308843D0 (en) | 2013-03-14 | 2013-07-03 | Verenium Corp | Phytase formulation |
| CN103159531A (zh) * | 2013-04-17 | 2013-06-19 | 广西新方向化学工业有限公司 | 一种含多种植物醇和中微量元素螯合的液体肥料及其制备方法 |
| US10093909B2 (en) | 2013-07-25 | 2018-10-09 | Basf Enzymes Llc | Phytase |
| WO2016077457A1 (en) | 2014-11-11 | 2016-05-19 | Clara Foods Co. | Methods and compositions for egg white protein production |
| CN104928271A (zh) * | 2015-07-06 | 2015-09-23 | 唐亚军 | 一种木聚糖酶的制备方法 |
| CN105200101B (zh) * | 2015-10-19 | 2019-04-16 | 华中农业大学 | 一种毕赤酵母菌体重复发酵表达外源蛋白的方法 |
| DK3997118T3 (da) | 2019-07-11 | 2025-03-24 | Clara Foods Co | Drikkevaresammensætning, der indeholder rekombinant ovomucoid-protein |
| US12096784B2 (en) | 2019-07-11 | 2024-09-24 | Clara Foods Co. | Protein compositions and consumable products thereof |
| US10927360B1 (en) | 2019-08-07 | 2021-02-23 | Clara Foods Co. | Compositions comprising digestive enzymes |
| WO2025077804A1 (en) * | 2023-10-12 | 2025-04-17 | Chengdu Kanghua Biological Products Co., Ltd. | Methanol monitoring method for the fermentation process of methanotrophic yeast |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0173378B1 (en) | 1984-07-27 | 1991-06-12 | Unilever N.V. | Process for preparing a polypeptide by culturing a transformed microorganism, a transformed microorganism suitable therefor and dna sequences suitable for preparing such microorganism |
| US4855231A (en) | 1984-10-30 | 1989-08-08 | Phillips Petroleum Company | Regulatory region for heterologous gene expression in yeast |
| NL8601454A (nl) * | 1986-06-05 | 1988-01-04 | Unilever Nv | Werkwijze voor het bereiden van een catalase-vrij oxidoreductase en van een catalase-vrije oxidoreductase bevattende gist, en gebruik daarvan. |
| EP0242007B1 (en) * | 1986-11-24 | 1990-11-07 | Unilever N.V. | Process for preparing a catalase-free oxidase and a catalase-free oxidase-containing yeast, and use thereof |
| EP0299108B1 (en) | 1987-07-17 | 1994-05-18 | Rhein Biotech Gesellschaft für biotechnologische Prozesse und Produkte mbH | DNA-molecules coding for FMDH control regions and structured gene for a protein having FMDH-activity and their uses |
| IL91765A0 (en) * | 1988-09-26 | 1990-06-10 | Salk Inst Biotech Ind | Mixed feed recombinant yeast fermentation |
| US5667986A (en) * | 1989-10-18 | 1997-09-16 | Delta Biotechnology Limited | Yeast promoter for expressing heterologous polypeptides |
| AU657168B2 (en) * | 1991-09-18 | 1995-03-02 | Amgen, Inc. | Hepatitis B vaccine with bile salt adjuvant |
| DE69535097T2 (de) | 1994-04-25 | 2006-12-28 | Dsm Ip Assets B.V. | Polypeptide mit phytase Wirkung |
| US6068995A (en) * | 1994-10-25 | 2000-05-30 | Yoshitomi Pharmaceutical Industries, Ltd. | Method for producing protein |
| KR0161148B1 (ko) * | 1995-10-11 | 1998-11-16 | 김은영 | 재조합 메탄올 자화 효모를 사용한 이종 단백질의 생산방법 |
| CA2231948C (en) | 1997-03-25 | 2010-05-18 | F. Hoffmann-La Roche Ag | Modified phytases |
-
1998
- 1998-09-25 EP EP98118241A patent/EP0911416B1/en not_active Expired - Lifetime
- 1998-09-25 EP EP06001709A patent/EP1659173A1/en not_active Withdrawn
- 1998-09-25 ES ES98118241T patent/ES2264181T3/es not_active Expired - Lifetime
- 1998-09-25 DE DE69834533T patent/DE69834533T2/de not_active Expired - Lifetime
- 1998-09-25 DK DK98118241T patent/DK0911416T3/da active
- 1998-09-29 CA CA002249061A patent/CA2249061C/en not_active Expired - Lifetime
- 1998-09-29 IN IN2187MA1998 patent/IN188151B/en unknown
- 1998-09-29 JP JP27410498A patent/JP4162774B2/ja not_active Expired - Lifetime
- 1998-09-29 US US09/163,052 patent/US6204012B1/en not_active Expired - Lifetime
- 1998-09-30 BR BRPI9803807-9A patent/BR9803807B1/pt not_active IP Right Cessation
- 1998-09-30 CN CNB98118992XA patent/CN1215175C/zh not_active Expired - Lifetime
- 1998-09-30 KR KR1019980041127A patent/KR100572426B1/ko not_active Expired - Lifetime
- 1998-09-30 AU AU87157/98A patent/AU748187B2/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| US6204012B1 (en) | 2001-03-20 |
| IN188151B (enExample) | 2002-08-24 |
| JP4162774B2 (ja) | 2008-10-08 |
| BR9803807B1 (pt) | 2010-11-03 |
| JPH11169173A (ja) | 1999-06-29 |
| EP0911416B1 (en) | 2006-05-17 |
| EP0911416A2 (en) | 1999-04-28 |
| AU748187B2 (en) | 2002-05-30 |
| KR19990036734A (ko) | 1999-05-25 |
| BR9803807A (pt) | 2000-03-28 |
| EP0911416A3 (en) | 2000-01-19 |
| DE69834533D1 (de) | 2006-06-22 |
| CA2249061A1 (en) | 1999-04-01 |
| ES2264181T3 (es) | 2006-12-16 |
| CN1218112A (zh) | 1999-06-02 |
| CA2249061C (en) | 2009-02-24 |
| EP1659173A1 (en) | 2006-05-24 |
| KR100572426B1 (ko) | 2006-06-21 |
| DK0911416T3 (da) | 2006-08-14 |
| DE69834533T2 (de) | 2006-10-12 |
| AU8715798A (en) | 1999-04-22 |
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