CN1214857A - Method for cultivating onion male sterile asexual line and conservation line - Google Patents

Method for cultivating onion male sterile asexual line and conservation line Download PDF

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Publication number
CN1214857A
CN1214857A CN 98119832 CN98119832A CN1214857A CN 1214857 A CN1214857 A CN 1214857A CN 98119832 CN98119832 CN 98119832 CN 98119832 A CN98119832 A CN 98119832A CN 1214857 A CN1214857 A CN 1214857A
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onion
male sterile
clone
maintenance line
bulb
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CN1108746C (en
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蒋钟仁
李春玲
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BEIJING HAIDIAN PLANT ORGANISM CULTURE TECHNIQUE LABAROTORY
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BEIJING HAIDIAN PLANT ORGANISM CULTURE TECHNIQUE LABAROTORY
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Abstract

The present invention relates to a method for preparing male sterile line and maintainer line of common onion by using plant tissue culture method. It is characterized by using plant tissue culture method to make common onion male sterile single plant propagation to obtain its male sterile clone, and using the obtained male sterile clone and the propagative bulb plant of common onion of same variety to make propagation to obtain the common onion sterile line and maintainer line. Its sterility rate is 93.5-97.5%.

Description

Set up the male sterile clone of onion and the method for maintenance line
The present invention relates to a kind ofly set up the clonal method of onion male sterile, and on the basis of this technology, set up the male sterile clone of onion and the method for maintenance line with the Plant Tissue Breeding method.
Onion is one of important vegetables.In China, because onion is easy to plantation, can be stored to vegetables supply the market in dull season, multiple eating method and medical care effect and obtain larger area and plant are widely arranged.Onion is to be product with the trophosome bulb, and its crossbreed ought to have than remarkable advantages, but up to the present, China does not still have the onion crossbreed to use aborning.Expect the onion crossbreed, must obtain the male sterile clone and the maintenance line corresponding of onion kind with it.China has numerous onion improved seeds, such as: Beijing Calusena lansium, the purple skin in Beijing, Nanjing Calusena lansium etc., almost all having the male sterile individual plant to exist in each onion kind, is very difficult and will set up male sterile line and find its corresponding maintenance line from sterile individual plant.
Purpose of the present invention is exactly to utilize the Plant Tissue Breeding method to set up the method for onion male sterile clone and maintenance line.
The technical scheme of this invention is: the method for tissue culture that utilizes plant, onion male sterile individual plant is expanded numerous male sterile clone that is, breed male sterile line and the maintenance line that obtains onion with the educated bulb ball strain of male sterile clone and same kind onion.
Concrete steps are as follows:
Choose the seed collecting strain of WUHUAFEN in the flower pesticide flowering stage in onion seed collecting field, its whole strain is pulled up get back to the laboratory.With aceto-camine flower pesticide is done microscopy, only examine under a microscope seed collecting strain, just can be defined as the male sterile individual plant less than live pollen or WUHUAFEN.
Will confirm as the bulb of seed collecting strain bottom of male sterile individual plant downcut, promptly from foundation portion upwards 10 to 15 centimeters bulb is downcut.Removing bulb multilayer crust rinses well with running water again, use 70% alcohol-pickled whole bulb 30 minutes then, soaked 1 hour with saturated bleaching powder supernatant again, with 1% potassium permanganate soak 15 minute thereafter, soaked 12 minutes with 0.3% mercuric chloride the back again, at last on superclean bench with aseptic water washing for several times, just can prepare to inoculate.
The bulb that disinfects is put into the culture dish of filter paper, bulb top layer moisture content is blotted by filter paper.To carry instrument sterilization and coolings such as sloping sheet, knife, tweezers, the first half and periphery with bulb cuts away then, the lateral bud of its inner band rhizome base portion is cut into each rectangle explant of 1 centimetre high 2 centimetres of length and width, cut the cross otch again from the top, subsequently explant is inoculated in onion fast breeding culture medium 1 or 2.(medium composition see Table 1 and table 2)
Through 20 to 40 days cultivation, lateral bud was transferred in the onion fast breeding culture medium 1 or 2 of new preparation subsequently by growing in the explant, can grow up to the bud of growing thickly through 20 to 30 days again under non-staining situation, just can expand once numerous in per thereafter about 20 days.
Change in onion root media 1 or 2 expanding numerous good onion set one clump a clump (every clump 4 to 5 strain).(the medium composition sees Table 3 and table 4.Under 25 ℃ of left and right sides conditions of temperature, just can take root through 20 to 30 days.
To and transplant and use the sandy soil sterilization flowerpot before transplanting.During transplanting onion set is taken out from blake bottle, put into warm water and the medium of root removed clean, onion set one Cong Cong is planted bury then, water sufficient water after planting, be placed on night 15 ℃ of left and right sides and cultivate under about 25 ℃ the condition daytime.Through 30 to 40 days cultivation, the onion set look is dark green and growing way is vigorous, had shown in sandy soil transplant survival.
Table 1 onion fast breeding culture medium 1 (mg/l)
??????????NH 4NO 3 ??????1650-1750
???????CaCL 2·2H 2O ??????400-520
???????MgSO 4·7H 2O ??????310-410
???????????KNO 3 ??????1400-2100
??????????KH 2PO 4 ??????140-210
???????ZnSO 4·7H 2O ??????9.5-11.5
??????????H 3BO 3 ??????8.0-11.0
???????MnSO 4·4H 2O ??????22-29
???????CuSO 4·5H 2O ??????0.02-0.03
??????Na 2MoO 4·2H 2O ??????0.2-0.3
????C 10H 14N 2O 8Na 2·2H 2O ??????36.5-38.5
???????FeSO 4·7H 2O ??????27.0-28.5
???????????VB 1 ??????0.4-0.8
???????VB 6 ??????0.3-1.0
???????VB 9 ??????0.4-0.8
Inositol ??????80-120
Nicotinic acid ??????3-7
Glycine ??????1-5
Vitamin h (H) ??????0.02-0.07
???Kinetin/6-BA ??????2-8
?????NAA/IAA ??????0.3-0.8
Sucrose ???????30000
Agar ???????8000
???????PH ???????5.8
Table 2 onion fast breeding culture medium 2 (mg/l)
???????KNO 3 ??????1400-2100
??????NH 4NO 3 ??????1550-1750
????CaCL 2·2H 2O ??????400-520
?????MgSO 4.7H 2O ??????310-410
???????KH 2PO 4 ??????140-210
????MnSO 4·4H 2O ??????22-29
????ZnSO 4·7H 2O ??????9.5-11.5
????CuSO 4·5H 2O ??????0.02-0.03
???????H 3BO 3 ??????5.6-8.0
???Na 2MoO 4·2H 2O ??????0.2-0.3
?????????KI ??????0.6-0.9
????COCL 2·6H 2O ??????0.02-0.03
?C 10H 14N 2O 8Na 2·2H 2O ??????36.5-38.5
????FeSO 4·7H 2O ??????27.0-28.5
?????????VB 1 ??????0.3-0.9
???????VB 6 ??????0.3-1.0
Nicotinic acid ??????0.5-5.0
Glycine ??????1.0-4.0
Inositol ??????80-120
???Kinetin/6-BA ??????2-8
?????NAA/IAA ??????0.3-0.8
Sucrose ??????30000
Agar ??????8000
???????PH ??????5.8
Table 3 onion root media 1 (mg/l)
????????NH 4NO 3 ?????1650-1750
?????CaCL 2·2H 2O ?????400-520
?????MgSO 4·7H 2O ?????310-410
?????????KNO 3 ?????1400-2100
????????KH 2PO 4 ?????140-210
?????ZnSO 4·7H 2O ?????9.5-11.5
????????H 3BO 3 ?????8.0-11.0
?????MnSO 4·4H 2O ?????22-29
?????CuSO 4·5H 2O ?????0.02-0.03
????Na 2MoO 4·2H 2O ?????0.2-0.3
?C 10H 14N 2O 8Na 2·2H 2O ?????36.5-38.5
?????FeSO 4·7H 2O ?????27.0-28.5
??????????VB 1 ?????0.4-0.8
??????????VB 6 ?????0.3-1.0
??????????VB 9 ?????0.4-0.8
Inositol ?????80-120
Nicotinic acid ?????3-7
Glycine ???????1-5
Vitamin h (H) ???????0.02-0.07
???Kinetin/6-BA ???????2-8
?????NAA/IAA ???????0.3-0.8
Activated carbon ???????2500-5000
Sucrose ???????30000
Agar ???????8000
???????PH ???????5.8
Table 4 onion root media 2 (mg/l)
?????????KNO 3 ?????1400-2100
????????NH 4NO 3 ?????1550-1750
?????CaCL 2·2H 2O ?????400-520
?????MgSO 4·7H 2O ?????310-410
???????KH 2PO 4 ?????140-210
?????MnSO 4·4H 2O ?????22-29
?????ZnSO 4·7H 2O ?????9.5-11.5
?????CuSO 4·5H 2O ?????0.02-0.03
????????H 3BO 3 ?????5.6-8.0
????Na 2MoO 4·2H 2O ?????0.2-0.3
??????????KI ?????0.6-0.9
?????COCL 2·6H 2O ?????0.02-0.03
??C 10H 14N 2O 8Na 2·2H 2O ?????36.5-38.5
??????FeSO 4·7H 2O ?????27.0-28.5
??????????VB 1 ?????0.3-0.9
??????????VB 6 ?????0.3-1.0
Nicotinic acid ?????0.5-5.0
Glycine ?????1.0-4.0
Inositol ????80-120
???Kinetin/6-BA ????2-8
?????NAA/IAA ????0.3-0.8
Activated carbon ????2500-5000
Sucrose ????30000
Agar ????8000
???????PH ????5.8
No matter the onion tissue cultivating seedling that cultivates with the method is seedling or bulb ball, all shows as male sterile after blooming, and hence one can see that, and the male sterile clone is built up.
To collect with the clonal single bulb ball of onion male sterile that group training method obtains, obtain onion bulb stem ball (the bulb ball of promptly obtaining is wanted and the sterile material obtained is same kind) from the same place of choosing sterile individual plant at that time, quantity is decided according to sterile bulb ball, and ratio is 1: 1.This bulb ball of directly obtaining is for can educate strain.Sterile strain planted in twos with the bulb ball that can educate strain be in the same place, the cultivation management of planting ball is with generally onion seed collecting field is identical.
Plant the strain bolting phase, carry out gauze every pair of kind strain is covered on together, manufacture gauze and generally get final product with thinner window screening.When just spending, to carry out supplementary pollination.
Plucking seed must in time can not allow seed at will be scattered, because it is very important to next step seed selection to confirm that seed picks up from that strain.Must number take seed by the volume strain, for example the sterile strain of every pair of kind strain is compiled and is 1-A, and the educated strain of its correspondence then should be compiled and is 1-B.Second pair of corresponding volume of kind strain is 2-A and 2-B.The rest may be inferred.Because will add up and compare, so will all sow and the gained seedling all kept and plant to the seed that picks up from sterile strain to sterile rate.Also to all sow from the seed that can educate strain.
In flowering stage, statistics is from the sterile rate of sterile strain seedling in every pair of kind strain.With the highest several to staying of sterile rate, other pull out.For example, the sterile rate of the colony of 1-A is 75%, and the colony of 3-A is 71%, and the A colony of other strain number all is less than at 70% o'clock, can only stay 1-A and 3-A, and other paired colony is pulled out.Do following work subsequently in the colony of 1-A and 3-A: (1) with first strain of first strain among the 1-A and 1-B, promptly 1-A-1 and 1-B-1 (or other strain) isolate pollination in pairs.Equally, 1-A-2 and 1-B-1 (or other strain) can be isolated pollination or the like in pairs ... the rest may be inferred.(2) 1-B-1 and 1-B-2 etc. being done selfing reserves seed for planting.
Sowing from kind strains such as 1-A-1 and 1-B-1 adopt the numbering seed.All all kinds of arrangements such as its previous generation, i.e. that generation of 1-A and 1-B.
If add up the result of sterile rate be: the colony of 1-A-1 is 90.2%, and the colony of 3-A-5 is 97.5%, and other colony is lower than at 90% o'clock, can only keep the colony of 3-A-5, and other colony is eliminated.At this moment, 3-A-5 is a male sterile line, and 3-B-5 is its maintenance line.If also dissatisfied, can further look for the tool more male sterile line and the maintenance line of high infertility rate as stated above to this sterile rate.
Utilize method for plant tissue culture can obtain sterile rate and be 93.5-97.5% onion male sterile line and maintenance line, male sterile line of breeding and maintenance line be breeding male sterile lines in a large number, utilizes male sterile line and the male parent kind through filtering out can produce crossbreed.
Embodiment 1
Bear high mountain onion has fabulous storage endurance, and present embodiment selects for use bear high mountain onion to expand the breeding male male sterile line.In bear high mountain onion flowering stage, choose the seed collecting strain of WUHUAFEN in the flower pesticide in its seed collecting field, its whole strain is pulled up, get back to the laboratory, with aceto-camine flower pesticide is done microscopy, only examine under a microscope seed collecting strain, just can be confirmed as the male sterile individual plant less than live pollen or WUHUAFEN.Will confirm as the bulb of seed collecting strain bottom of male sterile individual plant downcut, promptly bulb is downcut from the 10cm that makes progress of foundation portion.Removing bulb multilayer crust rinses well with running water again, use 30 fens kinds of 70% alcohol-pickled whole bulb then, soaked 1 hour with saturated bleaching powder supernatant again, with 1% potassium permanganate soak 15 fen kind thereafter, soaked 12 minutes with 0.3% mercuric chloride the back again, at last on the superclean bench with aseptic water washing for several times after, prepare inoculation.The bulb that disinfects is put into the culture dish of filter paper, bulb top layer moisture content is blotted by filter paper.With sterilization of instruments such as slide, knife, tweezers and cooling, the first half and periphery with bulb cuts away then, the lateral bud of its inner band rhizome base portion is cut into each 1cm of length and width, high 2cm rectangle explant, cut the cross otch again from the top, then explant is inoculated into (the medium composition sees Table 1) in the onion fast breeding culture medium 1.Growing in through 20-40 days cultivation lateral bud under the non-staining situation by explant, transfer to subsequently in the onion fast breeding culture medium 1 of new preparation, grew up to the bud of growing thickly through 20-30 days again, expansion in per thereafter about 20 days is once numerous.Change (composition sees Table 3) in the onion root media 1 over to expanding numerous good onion set one clump a clump (every clump of 4-5 strain).Under 20 ℃ of left and right sides conditions of temperature, took root through 20-30 days.Then onion set is taken out from blake bottle, put into warm water the medium of root is removed totally, plant through in the sandy soil of sterilization.Water sufficient water after planting, be placed on 15 ℃ of nights, cultivate under 25 ℃ the condition daytime, through to cultivate the onion set look in 30-40 days be dark green and growing way is vigorous for having survived.No matter be seedling or bulb ball, all show as male sterile after blooming, hence one can see that, and the male sterile clone is built up.To obtain the onion bulb stem ball from the same place of choosing sterile individual plant at that time with the clonal single bulb ball collection of the onion male sterile that group training method obtains, quantity is decided according to sterile bulb ball, and ratio is 1: 1.Sterile strain planted in twos with the bulb ball that can educate strain be in the same place, the cultivation management of planting ball is with generally onion seed collecting field is identical.Plant the strain bolting phase, every pair of kind strain is covered on together, when just spending, in each screen covering, put into some artificial feeding flies, to carry out supplementary pollination with gauze.In time pluck behind the seed maturity and by the numbering seed of gathering.All sow by seed of numbering the sterile strain that all to gather and the seed that can educate strain then.Statistics is from the sterile rate of sterile strain seedling in every pair of kind strain, and high several to staying of sterile rate, all the other are pulled out.The paired isolation pollination that stays, seed selection so constantly is till choosing desirable sterile rate.Obtain sterile rate at last and be 93.5% bear high mountain onion male sterile line and maintenance line.Utilize the male sterile line of bear high mountain onion to do female parent, do male parent through Beijing yellow onion of screening and can produce than maternal and the better onion crossbreed of male parent varieties and characteristics, output is male parent volume increase 16%, than female parent volume increase 21%.
Embodiment 2
Beijing yellow onion has quality and yielding ability preferably, and present embodiment selects for use the male sterile individual plant of Beijing yellow onion to expand the sterile clone of breeding male.Choose the male sterile individual plant with method similarly to Example 1.And the bulb of confirmed male sterile individual plant bottom downcut, promptly bulb is downcut from the 15cm that makes progress of foundation portion.With similarly to Example 1 method to the bulb disinfection of downcutting with carry out explant and be inoculated in the onion fast breeding culture medium 2.(the medium composition sees Table 2)
Through nearly 20-40 days cultivation, lateral bud was transferred in the onion fast breeding culture medium 2 of new preparation subsequently by growing in the explant, grew up to the bud of growing thickly through 20-30 days again, and expansion in per thereafter about 20 days is once numerous.Change (composition sees Table 4) in the onion root media 2 over to expanding one clump of the every 4-5 strain of numerous good onion set.Under 25 ℃ of left and right sides conditions of temperature, took root through 20-30 days.Onion set after taking root is planted in the sandy soil.Water after planting, be placed on about 15 ℃ of nights, cultivate under 25 ℃ the condition daytime, and it is dark green that process was cultivated the onion set look in 30-40 days and growing way is vigorous, shows transplant survival.No matter be seedling or bulb ball, all show as male sterile after blooming.The clonal single bulb ball of onion male sterile that will obtain with group training method and the educated bulb ball of same kind be planted together in twos, use the method identical with embodiment 1 to obtain male sterile line and the maintenance line that sterile rate is Beijing yellow onion of 97.5%.Utilize the male sterile line of Beijing yellow onion to do female parent, do male parent through the purple skin onion in Beijing of screening and can produce than maternal and the better onion crossbreed of male parent varieties and characteristics, the production ratio male parent increases 24%, increases 11% than female parent.
The method of embodiment 3-embodiment 4 usefulness embodiment 1, from purple skin in Beijing and Nanjing Calusena lansium, select the male sterile individual plant respectively, use method identical and condition that its individual plant is carried out the plant division cultivation then and root system is cultivated, set up the male sterile clone with embodiment 1.Use the method identical with embodiment 1 to select for use educated strain separately to do male parent then, pairing in twos obtains male sterile line and the maintenance line of the purple skin onion in Beijing of sterile rate 95.5% respectively, and sterile rate is the male sterile line and the maintenance line of 94.5% Nanjing yellow onion.Utilize purple skin onion male sterile line in Beijing and Nanjing yellow onion male sterile line to do female parent respectively, bear high mountain onion through screening is cooked male parent, can produce than maternal and the better onion crossbreed of male parent varieties and characteristics, output increases 19% and 22% than male parent respectively, increases 17% and 26% than female parent.

Claims (9)

1. method of setting up onion male sterile clone and maintenance line, it is characterized in that: utilize method for plant tissue culture that onion male sterile individual plant is expanded numerous male sterile clone that is, breed male sterile line and the maintenance line that obtains onion with the educated bulb ball strain of male sterile clone and same kind onion;
2. a kind of method of setting up onion male sterile clone and maintenance line according to claim 1, it is characterized in that: choosing of male sterile individual plant is the kind strain of choosing WUHUAFEN in the flower pesticide, with aceto-camine flower pesticide is dyeed then, less than pollen or the WUHUAFEN of living, confirm to have found the male sterile individual plant with microscopic examination thus;
3. a kind of method of setting up onion male sterile clone and maintenance line according to claim 1, it is characterized in that: the bulb of male sterile individual plant bottom is downcut, remove dephosphorization stem multilayer crust under aseptic condition with 70% alcohol-pickled, soak with saturated bleaching powder supernatant then, with 1% potassium permanganate soak, again with being inoculated on the onion fast breeding culture medium behind the 0.3% mercuric chloride liquid disinfectant thereafter;
4. a kind of method of setting up onion male sterile clone and maintenance line according to claim 3, it is characterized in that: the bulb before the sterilization should be from the make progress 10-15cm cutting-out of foundation portion, bulb after the sterilization cuts away the first half and periphery, length and width 1cm is cut into the lateral bud of rhizome base portion in inside, the rectangle explant of high 2cm is cut a cross otch again from the top;
5. according to the described a kind of method of setting up onion male sterile clone and maintenance line of claim 3, it is characterized in that: the onion fast breeding culture medium is to constitute (mg/l) NH by following composition 4NO 31650-1750
CaCL 2·2H 2O????????????400-520
MgSO 4·7H 2O????????????310-410
KNO 3????????????????????1400-2100
KH 2PO 4??????????????140-210
ZnSO 4·7H 2O????????????9.5-11.5
H 3BO 3??????????????????8.0-11.0
MnSO 4·4H 2O????????????22-29
CuSO 4·5H 2O????????????0.02-0.03
Na 2MoO 4·2H 2O???????0.2-0.3C 10H 14N 2O 8Na 2·2H 2O????36.5-38.5
FeSO 4·7H 2O????????????27.0-28.5
VB 1?????????????????0.4-0.8
VB 6?????????????????0.3-1.0
VB 9?????????????????0.4-0.8
Inositol 80-120
Nicotinic acid 3-7
Glycine 1-5
Vitamin h (H) 0.02-0.07 Kinetin/6-BA 2-8
NAA/IAA????????????????0.3-0.8
Sucrose 30000
Agar 8000
PH???????????????????5.8
6. a kind of method of setting up onion male sterile clone and maintenance line according to claim 3 is characterized in that: the onion fast breeding culture medium is to constitute (mg/l) by following composition
KNO 3??????????????????1400-2100
NH 4NO 3????????????????1550-1750?CaCL 2·2H 2O??????????????400-520?MgSO 4·7H 2O??????????????310-410
KH 2PO 4????????????????140-210?MnSO 4·4H 2O??????????????22-29?ZnSO 4·7H 2O??????????????9.5-11.5?CuSO 4·5H 2O??????????????0.02-0.03
H 3BO 3?????????????????5.6-8.0Na 2MoO 4·2H 2O???????????0.2-0.3?????KI???????????????????0.6-0.9
COCL 2·6H 2O???????????0.02-0.03C 10H 14N 2O 8Na 2·2H 2O??36.5-38.5
FeSO 4·7H 2O???????????27.0-28.5
VB 1???????????????0.3-0.9
VB 6???????????????0.3-1.0
Nicotinic acid 0.5-5.0
Glycine 1.0-4.0
Inositol 80-120
Kinetin/6-BA??????????2-8
NAA/IAA?????????????0.3-0.8
Sucrose 30000
Agar 8000
PH????????????????5.8
7. a kind of method of setting up onion male sterile clone and maintenance line according to claim 3 is characterized in that: the onion root media is to constitute (mg/l) by following composition
NH 4NO 3????????????????????1650-1750
CaCL 2·2H 2O??????????????????400-520
MgSO 4·7H 2O?????????????????310-410
KNO 3??????????????????????1400-2100
KH 2PO 4????????????????????140-210
ZnSO 4·7H 2O?????????????????9.5-11.5
H 3BO 3?????????????????????8.0-11.0
MnSO 4·4H 2O??????????????????22-29
CuSO 4·5H 2O??????????????????0.02-0.03???Na 2MoO 4·2H 2O????????????????0.2-0.3C 10H 14N 2O 8Na 2·2H 2O?????????36.5-38.5
FeSO 4·7H 2O??????????????????27.0-28.5
VB 1???????????????0.4-0.8
VB 6???????????????0.3-1.0
VB 9???????????????0.4-0.8
Inositol 80-120
Nicotinic acid 3-7
Glycine 1-5
Vitamin h (H) 0.02-0.07
Kinetin/6-BA??????????2-8
NAA/IAA?????????????0.3-0.8
Activated carbon 2500-5000
Sucrose 30000
Agar 8000
PH????????????????5.8
8. a kind of method of setting up onion male sterile clone and maintenance line according to claim 3 is characterized in that: the onion root media is to constitute (mg/l) by following composition
KNO 3????????????????1400-2100
NH 4NO 3???????????????1550-1750
CaCL 2·2H 2O?????????????400-520
MgSO 4·7H 2O?????????????310-410
KH 2PO 4???????????????140-210
MnSO 4·4H 2O?????????????22-29
ZnSO 4·7H 2O?????????????9.5-11.5
CuSO 4·5H 2O?????????????0.02-0.03
H 3BO 3????????????????5.6-8.0???Na 2MoO 4·2H 2O???????????0.2-0.3
KI???????????????????0.6-0.9
COCL 2·6H 2O?????????????0.02-0.03C 10H 14N 2O 8Na 2·2H 2O????36.5-38.5
FeSO 4·7H 2O????27.0-28.5
VB 1????????????0.3-0.9
VB 60.3-1.0 nicotinic acid 0.5-5.0 glycine 1.0-4.0 inositol 80-120Kinetin/6-BA 2-8 NAA/IAA 0.3-0.8 activated carbon 2500-5000
Sucrose 30000
Agar 8000
PH????????????5.8
9. a kind of method of setting up onion male sterile clone and maintenance line according to claim 1, it is characterized in that: the single bulb strain of male sterile clone that will cultivate and the educated bulb ball of same kind are planted together in twos, isolate the pollination breed, with the paired planting seed of gathering, select high the staying of sterile rate to continue to isolate the pollination breed then, push away according to this and plant till choosing satisfied sterile rate seed, breed male sterile line and the maintenance line of onion with the method.
CN 98119832 1998-09-18 1998-09-18 Method for cultivating onion male sterile asexual line and conservation line Expired - Lifetime CN1108746C (en)

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Publication number Priority date Publication date Assignee Title
CN101960991A (en) * 2010-11-15 2011-02-02 南京农业大学 Onion callus induction method and special culture medium thereof
CN102726284A (en) * 2011-04-08 2012-10-17 北京市农林科学院 Breeding method for onion petaloid-stamen type male sterile line
CN102870674A (en) * 2012-09-05 2013-01-16 广州白云华南生物科技有限公司 Method for quickly propagating red onion through tissue culture
CN106417000A (en) * 2016-09-27 2017-02-22 云南省农业科学院花卉研究所 Pollen-free lily variety breeding method

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101960991A (en) * 2010-11-15 2011-02-02 南京农业大学 Onion callus induction method and special culture medium thereof
CN102726284A (en) * 2011-04-08 2012-10-17 北京市农林科学院 Breeding method for onion petaloid-stamen type male sterile line
CN102870674A (en) * 2012-09-05 2013-01-16 广州白云华南生物科技有限公司 Method for quickly propagating red onion through tissue culture
CN102870674B (en) * 2012-09-05 2013-12-25 广州白云华南生物科技有限公司 Method for quickly propagating red onion through tissue culture
CN106417000A (en) * 2016-09-27 2017-02-22 云南省农业科学院花卉研究所 Pollen-free lily variety breeding method
CN106417000B (en) * 2016-09-27 2018-07-06 云南省农业科学院花卉研究所 A kind of breeding method of no pollen lily cultivar

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