CN120829942A - 酶促rna合成 - Google Patents

酶促rna合成

Info

Publication number
CN120829942A
CN120829942A CN202510727733.3A CN202510727733A CN120829942A CN 120829942 A CN120829942 A CN 120829942A CN 202510727733 A CN202510727733 A CN 202510727733A CN 120829942 A CN120829942 A CN 120829942A
Authority
CN
China
Prior art keywords
polymerase
poly
oligonucleotide
certain embodiments
rna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202510727733.3A
Other languages
English (en)
Chinese (zh)
Inventor
G·M·彻奇
D·J·维甘德
R·E·科曼
E·库鲁
J·里蒂奇尔
N·康维
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard University
Original Assignee
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard University filed Critical Harvard University
Publication of CN120829942A publication Critical patent/CN120829942A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1282—RNA uridylyltransferase (2.7.7.52)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/322—2'-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/323—Chemical structure of the sugar modified ring structure
    • C12N2310/3231—Chemical structure of the sugar modified ring structure having an additional ring, e.g. LNA, ENA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07052—RNA uridylyltransferase (2.7.7.52)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
CN202510727733.3A 2018-10-12 2019-10-11 酶促rna合成 Pending CN120829942A (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201862745136P 2018-10-12 2018-10-12
US62/745,136 2018-10-12
CN201980082542.9A CN113195720B (zh) 2018-10-12 2019-10-11 酶促rna合成
PCT/US2019/055870 WO2020077227A2 (en) 2018-10-12 2019-10-11 Enzymatic rna synthesis

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201980082542.9A Division CN113195720B (zh) 2018-10-12 2019-10-11 酶促rna合成

Publications (1)

Publication Number Publication Date
CN120829942A true CN120829942A (zh) 2025-10-24

Family

ID=69232884

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202510727733.3A Pending CN120829942A (zh) 2018-10-12 2019-10-11 酶促rna合成
CN201980082542.9A Active CN113195720B (zh) 2018-10-12 2019-10-11 酶促rna合成

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201980082542.9A Active CN113195720B (zh) 2018-10-12 2019-10-11 酶促rna合成

Country Status (6)

Country Link
US (1) US20220145295A1 (https=)
EP (1) EP3864151A2 (https=)
JP (1) JP7646209B2 (https=)
KR (2) KR20260047628A (https=)
CN (2) CN120829942A (https=)
WO (1) WO2020077227A2 (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3826762A1 (en) 2018-07-23 2021-06-02 DNA Script Massively parallel enzymatic synthesis of nucleic acid strands
WO2020165137A1 (en) 2019-02-12 2020-08-20 Dna Script Efficient product cleavage in template-free enzymatic synthesis of polynucleotides.
WO2021018919A1 (en) * 2019-07-30 2021-02-04 Dna Script Template-free enzymatic synthesis of polynucleotides using poly(a) and poly(u) polymerases
WO2022013094A1 (en) 2020-07-15 2022-01-20 Dna Script Massively parallel enzymatic synthesis of polynucleotides
EP4334330A1 (en) 2021-05-07 2024-03-13 Helix Nanotechnologies, Inc. Modified ribonucleic acids and uses thereof
CA3234383A1 (en) 2021-10-15 2023-04-27 Codexis, Inc. Engineered terminal deoxynucleotidyl transferase variants
CN116732020A (zh) * 2022-03-02 2023-09-12 中国科学院分子细胞科学卓越创新中心 提高rna分子的胞内翻译效率和/或稳定性的方法
US20250207163A1 (en) * 2022-03-25 2025-06-26 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
EP4630540A1 (en) * 2022-12-05 2025-10-15 DNA Script Variants of poly(a) polymerase and uses thereof
WO2024141628A1 (en) 2022-12-31 2024-07-04 Dna Script Variable viscosity inks for inkjet delivery of enzyme reagents
WO2024153643A1 (en) 2023-01-16 2024-07-25 Dna Script Inkjet-assisted enzymatic nucleic acid synthesis
EP4731762A2 (en) * 2023-06-21 2026-04-29 Ansa Biotechnologies, Inc. De novo stepwise template-independent synthesis of long polynucleotides
CN118109539A (zh) * 2024-04-08 2024-05-31 华南理工大学 一种无模板酶法合成含非天然碱基的核糖核酸链的方法及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2290073A3 (en) * 2004-05-28 2011-08-31 Asuragen, Inc. Methods and compositions involving microRNA
WO2011038241A1 (en) 2009-09-25 2011-03-31 President And Fellows Of Harvard College Nucleic acid amplification and sequencing by synthesis with fluorogenic nucleotides
EP2551354A1 (en) * 2011-07-25 2013-01-30 Universität Heidelberg Functionalization of RNA oligonucleotides
US10683536B2 (en) * 2013-04-02 2020-06-16 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US9771613B2 (en) * 2013-04-02 2017-09-26 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acid

Also Published As

Publication number Publication date
WO2020077227A2 (en) 2020-04-16
CN113195720B (zh) 2025-06-20
JP2022504712A (ja) 2022-01-13
EP3864151A2 (en) 2021-08-18
US20220145295A1 (en) 2022-05-12
KR20210091153A (ko) 2021-07-21
JP7646209B2 (ja) 2025-03-17
KR102942644B1 (ko) 2026-03-24
WO2020077227A3 (en) 2020-08-27
KR20260047628A (ko) 2026-04-08
CN113195720A (zh) 2021-07-30

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