CN120699752A - 核酸检测系统、装置和方法 - Google Patents

核酸检测系统、装置和方法

Info

Publication number
CN120699752A
CN120699752A CN202510817026.3A CN202510817026A CN120699752A CN 120699752 A CN120699752 A CN 120699752A CN 202510817026 A CN202510817026 A CN 202510817026A CN 120699752 A CN120699752 A CN 120699752A
Authority
CN
China
Prior art keywords
hybridization
sequencing
amplification
fluid delivery
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202510817026.3A
Other languages
English (en)
Chinese (zh)
Inventor
克里斯特弗·图马佐
戴维·戴维森
山姆·里德
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DNAE Group Holdings Ltd
Original Assignee
DNAE Group Holdings Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DNAE Group Holdings Ltd filed Critical DNAE Group Holdings Ltd
Publication of CN120699752A publication Critical patent/CN120699752A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4146—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS involving nanosized elements, e.g. nanotubes, nanowires

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Computer Hardware Design (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202510817026.3A 2014-04-25 2015-04-27 核酸检测系统、装置和方法 Pending CN120699752A (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB1407334.0 2014-04-25
GBGB1407334.0A GB201407334D0 (en) 2014-04-25 2014-04-25 Integrated nucleic acid test system, instrument and method
CN201580021281.1A CN106460043A (zh) 2014-04-25 2015-04-27 核酸检测系统、装置和方法
PCT/EP2015/059097 WO2015162301A2 (en) 2014-04-25 2015-04-27 Nucleic acid test system, apparatus and method

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201580021281.1A Division CN106460043A (zh) 2014-04-25 2015-04-27 核酸检测系统、装置和方法

Publications (1)

Publication Number Publication Date
CN120699752A true CN120699752A (zh) 2025-09-26

Family

ID=50971895

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202510817026.3A Pending CN120699752A (zh) 2014-04-25 2015-04-27 核酸检测系统、装置和方法
CN201580021281.1A Pending CN106460043A (zh) 2014-04-25 2015-04-27 核酸检测系统、装置和方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201580021281.1A Pending CN106460043A (zh) 2014-04-25 2015-04-27 核酸检测系统、装置和方法

Country Status (7)

Country Link
US (2) US11312930B2 (enExample)
EP (1) EP3134544B1 (enExample)
JP (1) JP6684497B2 (enExample)
CN (2) CN120699752A (enExample)
ES (1) ES2870564T3 (enExample)
GB (1) GB201407334D0 (enExample)
WO (1) WO2015162301A2 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2017204294A1 (ja) * 2016-05-25 2019-02-21 株式会社ニコン 標的生体分子の特定方法、標的生体分子特定用ビーズ、ビーズのセット、及び標的生体分子特定装置
JP2017209051A (ja) * 2016-05-25 2017-11-30 株式会社ニコン 標的生体分子の検出方法、標的生体分子検出用基板、及び標的生体分子検出装置
GB2589159B (en) 2017-12-29 2023-04-05 Clear Labs Inc Nucleic acid sequencing apparatus
WO2019144966A1 (en) * 2018-01-29 2019-08-01 Coyote Bioscience Co., Ltd. Systems and methods for analyzing nucleic acids
US10467679B1 (en) 2019-04-15 2019-11-05 Dnanudge Limited Product recommendation device and method
US10811140B2 (en) 2019-03-19 2020-10-20 Dnanudge Limited Secure set-up of genetic related user account
US10699806B1 (en) 2019-04-15 2020-06-30 Dnanudge Limited Monitoring system, wearable monitoring device and method
WO2020211069A1 (en) * 2019-04-19 2020-10-22 Ardent Biomed Guangdong Co., Ltd Apparatus and method for high throughput parallel nucleic acid sequencing
CN119955908A (zh) * 2019-05-08 2025-05-09 Dnae诊断有限公司 组合的液相和固相dna扩增
US10942165B1 (en) 2019-12-20 2021-03-09 Dnae Group Holdings Ltd Methods for preparing cartridges for in vitro diagnostics and related systems
GB202014819D0 (en) * 2020-08-24 2020-11-04 Imperial College Innovations Ltd A method for determining a diagnostic outcome
CN112961768A (zh) * 2021-02-04 2021-06-15 海南微氪生物科技股份有限公司 一种Leeuwenhoek实时选择性微生物快速检测系统
US12009103B1 (en) * 2021-03-04 2024-06-11 Edward R Silansky Systems, devices, and/or methods for managing health
WO2024004917A1 (ja) * 2022-06-28 2024-01-04 京セラ株式会社 検出システム、カートリッジ、検出方法、核酸プローブの製造方法およびバイオセンサの製造方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6235471B1 (en) * 1997-04-04 2001-05-22 Caliper Technologies Corp. Closed-loop biochemical analyzers
US7071324B2 (en) * 1998-10-13 2006-07-04 Brown University Research Foundation Systems and methods for sequencing by hybridization
JP4395133B2 (ja) * 2002-12-20 2010-01-06 カリパー・ライフ・サイエンシズ・インク． Ｄｎａの単一分子増幅および検出
AU2007225038B2 (en) * 2006-03-15 2013-08-29 Revvity Health Sciences, Inc. Integrated nucleic acid assays
US8349167B2 (en) * 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US9096899B2 (en) 2010-10-27 2015-08-04 Illumina, Inc. Microdevices and biosensor cartridges for biological or chemical analysis and systems and methods for the same
GB201212775D0 (en) * 2012-07-18 2012-08-29 Dna Electronics Ltd Sensing apparatus and method

Also Published As

Publication number Publication date
ES2870564T3 (es) 2021-10-27
WO2015162301A2 (en) 2015-10-29
US11879116B2 (en) 2024-01-23
JP2017522858A (ja) 2017-08-17
US20170166848A1 (en) 2017-06-15
EP3134544A2 (en) 2017-03-01
US11312930B2 (en) 2022-04-26
JP6684497B2 (ja) 2020-04-22
GB201407334D0 (en) 2014-06-11
EP3134544B1 (en) 2021-02-17
CN106460043A (zh) 2017-02-22
WO2015162301A3 (en) 2016-02-04
US20220195355A1 (en) 2022-06-23

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