JP6684497B2 - 核酸検査のシステム、装置、及び方法 - Google Patents

核酸検査のシステム、装置、及び方法 Download PDF

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JP6684497B2
JP6684497B2 JP2016560950A JP2016560950A JP6684497B2 JP 6684497 B2 JP6684497 B2 JP 6684497B2 JP 2016560950 A JP2016560950 A JP 2016560950A JP 2016560950 A JP2016560950 A JP 2016560950A JP 6684497 B2 JP6684497 B2 JP 6684497B2
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fluid
amplification
sequencing
sensor
hybridization
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JP2017522858A (ja
JP2017522858A5 (enExample
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トウマゾウ，クリストファー
デビッドソン，デビッド
リード，サム
Original Assignee
ディーエヌエーイー グループ ホールディングス エルティーディー
ディーエヌエーイー グループ ホールディングス エルティーディー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4146—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS involving nanosized elements, e.g. nanotubes, nanowires

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Computer Hardware Design (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2016560950A 2014-04-25 2015-04-27 核酸検査のシステム、装置、及び方法 Active JP6684497B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1407334.0 2014-04-25
GBGB1407334.0A GB201407334D0 (en) 2014-04-25 2014-04-25 Integrated nucleic acid test system, instrument and method
PCT/EP2015/059097 WO2015162301A2 (en) 2014-04-25 2015-04-27 Nucleic acid test system, apparatus and method

Publications (3)

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JP2017522858A JP2017522858A (ja) 2017-08-17
JP2017522858A5 JP2017522858A5 (enExample) 2018-04-19
JP6684497B2 true JP6684497B2 (ja) 2020-04-22

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JP2016560950A Active JP6684497B2 (ja) 2014-04-25 2015-04-27 核酸検査のシステム、装置、及び方法

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US (2) US11312930B2 (enExample)
EP (1) EP3134544B1 (enExample)
JP (1) JP6684497B2 (enExample)
CN (2) CN120699752A (enExample)
ES (1) ES2870564T3 (enExample)
GB (1) GB201407334D0 (enExample)
WO (1) WO2015162301A2 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2017204294A1 (ja) * 2016-05-25 2019-02-21 株式会社ニコン 標的生体分子の特定方法、標的生体分子特定用ビーズ、ビーズのセット、及び標的生体分子特定装置
JP2017209051A (ja) * 2016-05-25 2017-11-30 株式会社ニコン 標的生体分子の検出方法、標的生体分子検出用基板、及び標的生体分子検出装置
GB2589159B (en) 2017-12-29 2023-04-05 Clear Labs Inc Nucleic acid sequencing apparatus
WO2019144966A1 (en) * 2018-01-29 2019-08-01 Coyote Bioscience Co., Ltd. Systems and methods for analyzing nucleic acids
US10467679B1 (en) 2019-04-15 2019-11-05 Dnanudge Limited Product recommendation device and method
US10811140B2 (en) 2019-03-19 2020-10-20 Dnanudge Limited Secure set-up of genetic related user account
US10699806B1 (en) 2019-04-15 2020-06-30 Dnanudge Limited Monitoring system, wearable monitoring device and method
WO2020211069A1 (en) * 2019-04-19 2020-10-22 Ardent Biomed Guangdong Co., Ltd Apparatus and method for high throughput parallel nucleic acid sequencing
CN119955908A (zh) * 2019-05-08 2025-05-09 Dnae诊断有限公司 组合的液相和固相dna扩增
US10942165B1 (en) 2019-12-20 2021-03-09 Dnae Group Holdings Ltd Methods for preparing cartridges for in vitro diagnostics and related systems
GB202014819D0 (en) * 2020-08-24 2020-11-04 Imperial College Innovations Ltd A method for determining a diagnostic outcome
CN112961768A (zh) * 2021-02-04 2021-06-15 海南微氪生物科技股份有限公司 一种Leeuwenhoek实时选择性微生物快速检测系统
US12009103B1 (en) * 2021-03-04 2024-06-11 Edward R Silansky Systems, devices, and/or methods for managing health
WO2024004917A1 (ja) * 2022-06-28 2024-01-04 京セラ株式会社 検出システム、カートリッジ、検出方法、核酸プローブの製造方法およびバイオセンサの製造方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6235471B1 (en) * 1997-04-04 2001-05-22 Caliper Technologies Corp. Closed-loop biochemical analyzers
US7071324B2 (en) * 1998-10-13 2006-07-04 Brown University Research Foundation Systems and methods for sequencing by hybridization
JP4395133B2 (ja) * 2002-12-20 2010-01-06 カリパー・ライフ・サイエンシズ・インク． Ｄｎａの単一分子増幅および検出
AU2007225038B2 (en) * 2006-03-15 2013-08-29 Revvity Health Sciences, Inc. Integrated nucleic acid assays
US8349167B2 (en) * 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US9096899B2 (en) 2010-10-27 2015-08-04 Illumina, Inc. Microdevices and biosensor cartridges for biological or chemical analysis and systems and methods for the same
GB201212775D0 (en) * 2012-07-18 2012-08-29 Dna Electronics Ltd Sensing apparatus and method

Also Published As

Publication number Publication date
ES2870564T3 (es) 2021-10-27
WO2015162301A2 (en) 2015-10-29
US11879116B2 (en) 2024-01-23
JP2017522858A (ja) 2017-08-17
US20170166848A1 (en) 2017-06-15
CN120699752A (zh) 2025-09-26
EP3134544A2 (en) 2017-03-01
US11312930B2 (en) 2022-04-26
GB201407334D0 (en) 2014-06-11
EP3134544B1 (en) 2021-02-17
CN106460043A (zh) 2017-02-22
WO2015162301A3 (en) 2016-02-04
US20220195355A1 (en) 2022-06-23

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