CN1193770C - 一种鹿茸药用成分的水解提取方法及产品 - Google Patents

一种鹿茸药用成分的水解提取方法及产品 Download PDF

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CN1193770C
CN1193770C CNB031108237A CN03110823A CN1193770C CN 1193770 C CN1193770 C CN 1193770C CN B031108237 A CNB031108237 A CN B031108237A CN 03110823 A CN03110823 A CN 03110823A CN 1193770 C CN1193770 C CN 1193770C
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CN1425387A (zh
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崔建华
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Benxi Huaxia real estate development limited liability company
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Abstract

一种鹿茸药用成分的水解提取方法，取新鲜的鹿茸加入缓冲液，用组织绞碎机绞碎，调pH值为1.0-3.0，将溶液的温度调至20℃-30℃，加入复合的生物催化剂进行水解，然后离心或硅藻土过滤，得到澄清金黄色的鹿茸水解液。鲜鹿茸经过水解方法处理后，其平均水解率达70%左右(水解率根据鲜鹿茸的质量不同在60-75%之间)；水解后的残余物为鹿茸钙化的部分及部分皮毛，所有的有机质基本都变成溶液状态，变成了易于人体吸收的溶液状态。得到的产品可应用于制药、保健品、食品的诸多方面，应用极其方便，同时利用本技术对鹿茸产品进行开发可衍生很多新的开发途径，得到系列新产品。

Description

一种鹿茸药用成分的水解提取方法及产品
技术领域：
本发明涉及中药有效部位成分的提取方法，尤其是提供一种鹿茸药用成分的水解提取方法及产品，属于中药有效成分提取技术领域。
背景技术：
虽然鹿茸有较好的医用价值和较强的保健作用，但由于鹿茸的组织结构独特一通过鹿茸组织结构研究表明，鹿茸(除茸皮外)从顶部到基部是由发育程度不同的骨组织构成；因此鹿茸中含有大量构成骨及前骨的材料—硫酸软骨素和胶原纤维，因此对鹿茸并没有好的利用方法；在传统的中医医疗中，鹿茸作为中药配伍的一部分，只能进行水煎，在利用过程中利用率只有2％左右，绝大部分都废弃；而作为传统的补品，一般只能用于泡酒等，利用率则更低。因此，传统鹿茸的利用方式，对鹿茸资源是很大的浪费。
发明内容：
本发明提供一种鹿茸药用成分的水解提取方法及产品，充分利用宝贵的鹿茸资源，更好的发挥鹿茸的药用价值。
本发明的技术解决方案包括以下步骤：
取新鲜的鹿茸加入缓冲液，用组织绞碎机绞碎，调溶液的pH值为1.0-10.0，按鹿茸重量的0.005～0.01加入生物催化剂，包括：胃蛋白酶、HAP碱性蛋白酶、木瓜蛋白酶、胰酶、碱性内切蛋白酶(Alcalase)、风味酶(Flavourzyme)六种酶中的一种或多种，同时加入Ca2+、Mg2+、Zn2+、Mg2+等氯化物酶激活剂，在温度20℃-60℃，pH1.0-pH10.0条件下进行水解反应2小时-5小时，然后离心或硅藻土过滤，得到澄清金黄色的鹿茸水解液，水解率在60％-70％。
缓冲液：磷酸盐、柠檬酸盐、乳酸盐缓冲液。
生物催化剂包括：胃蛋白酶、HAP碱性蛋白酶、木瓜蛋白酶、胰酶、碱性内切蛋白酶(Alcalase)、风味酶(Flavourzyme)。
激活剂：Ca2+、Mg2+、Zn2+、Mg2+等氯化物。
水解液中含有多种有效物质：经过分析，鹿茸水解后的溶液中含有大量的小分子多肽、脂类(主要有磷脂、甾体化合物、脂肪酸、中性脂和前列腺素等)、游离氨基酸、微量元素、糖类等多种有效成分。
本发明的积极效果在于：鲜鹿茸经过水解方法处理后，其平均水解率达70％左右(水解率根据鲜鹿茸的质量不同在60-75％之间)；水解后的残余物为鹿茸钙化的部分及部分皮毛，所有的有机质基本都变成溶液状态，鹿茸中几乎所有的有效成分都变成了易于人体吸收的溶液状态。得到的产品可应用于制药、保健品、食品的诸多方面，应用极其方便，同时利用本技术对鹿茸产品进行开发可衍生很多新的开发途径，得到系列新产品。
具体实施方式：
实施例1
取一定量新鲜的鹿茸1kg，加入3000ml磷酸盐的缓冲液，用组织绞碎机绞碎，加入生物催化剂：胃蛋白酶10g、激活剂氯化锌0.1g，调pH值为1.5，将溶液的温度调至25℃，放入反应罐反应4小时，离心去残渣，收集透明澄清的液体3200ml。
实施例2：
取一定量新鲜的鹿茸1kg，加入3000ml柠檬酸盐的缓冲液，用组织绞碎机绞碎，加入生物催化剂：木瓜蛋白酶8g、激活剂氯化钙0.08g，调pH值为7.0，将溶液的温度调至37℃，放入反应罐反应5小时，离心去残渣，收集透明澄清的液体3300ml。
实施例3
取一定量新鲜的鹿茸1kg，加入3000ml乳酸盐的缓冲液，用组织绞碎机绞碎，加入生物催化剂：胰酶10g、激活剂氯化锰0.15g，调pH值为9.0，将溶液的温度调至52℃，放入反应罐反应3小时，离心去残渣，收集透明澄清的液体3340ml。

Claims (1)

1、一种鹿茸药用成分的水解提取方法，包括以下步骤完成的：取新鲜的鹿茸加入缓冲液，用组织绞碎机绞碎，按比例为鹿茸重量的0.005-0.01加入生物催化剂，再加入氯化物作为激活剂，在温度20℃-60℃，pH1.0-pH10.0的条件下进行水解反应2小时-5小时，然后离心或硅藻土过滤；
所述的缓冲液选自磷酸盐缓冲液、柠檬酸盐缓冲液、乳酸盐缓冲液中的一种或多种；
所述的生物催化剂选自胃蛋白酶、HAP碱性蛋白酶、木瓜蛋白酶、胰酶、碱性内切蛋白酶、风味酶中的一种或多种；
所述的激活剂选自Ca2+、Mg2+、Zn2+、Mn2+的氯化物。
CNB031108237A 2003-01-09 2003-01-09 一种鹿茸药用成分的水解提取方法及产品 Expired - Fee Related CN1193770C (zh)

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CN1300324C (zh) * 2003-12-12 2007-02-14 长春工业大学 一种从鹿茸中提取纯化多糖的方法
CN101084925B (zh) * 2007-06-22 2011-08-10 王蓓蕾 一种鹿茸母液的生产工艺
CN101647568B (zh) * 2009-09-03 2012-09-05 辽宁鹿源参茸饮片有限公司 一种梅花鹿鹿茸液及其制备方法
CN101948439B (zh) * 2010-08-17 2012-08-22 刘平 鹿茸活性生物碱类化合物的提取方法及在医药上的应用
CN102286561B (zh) * 2011-07-11 2015-05-13 长春工业大学 一种鲜鹿茸酶降解提取有效成分的方法

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