CN119365607A - 具核梭杆菌的检测方法和具核梭杆菌具核亚种的检测方法 - Google Patents

具核梭杆菌的检测方法和具核梭杆菌具核亚种的检测方法 Download PDF

Info

Publication number
CN119365607A
CN119365607A CN202380049446.0A CN202380049446A CN119365607A CN 119365607 A CN119365607 A CN 119365607A CN 202380049446 A CN202380049446 A CN 202380049446A CN 119365607 A CN119365607 A CN 119365607A
Authority
CN
China
Prior art keywords
primer
seq
base sequence
region
primer set
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202380049446.0A
Other languages
English (en)
Chinese (zh)
Inventor
郭俊佑
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eiken Chemical Co Ltd
Original Assignee
Eiken Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eiken Chemical Co Ltd filed Critical Eiken Chemical Co Ltd
Publication of CN119365607A publication Critical patent/CN119365607A/zh
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y401/00Carbon-carbon lyases (4.1)
    • C12Y401/03Oxo-acid-lyases (4.1.3)
    • C12Y401/03006Citrate (pro-3S)-lyase (4.1.3.6)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202380049446.0A 2022-09-06 2023-09-05 具核梭杆菌的检测方法和具核梭杆菌具核亚种的检测方法 Pending CN119365607A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2022141680 2022-09-06
JP2022-141680 2022-09-06
PCT/JP2023/032374 WO2024053642A1 (ja) 2022-09-06 2023-09-05 フソバクテリウム・ヌクレアタムの検出方法及びフソバクテリウム・ヌクレアタム亜種ヌクレアタムの検出方法

Publications (1)

Publication Number Publication Date
CN119365607A true CN119365607A (zh) 2025-01-24

Family

ID=90191247

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202380049446.0A Pending CN119365607A (zh) 2022-09-06 2023-09-05 具核梭杆菌的检测方法和具核梭杆菌具核亚种的检测方法

Country Status (4)

Country Link
EP (1) EP4553165A1 (https=)
JP (1) JPWO2024053642A1 (https=)
CN (1) CN119365607A (https=)
WO (1) WO2024053642A1 (https=)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6299660B2 (ja) 2014-05-12 2018-03-28 三菱ケミカル株式会社 菌叢解析方法と菌叢解析用デバイス
JP6945294B2 (ja) 2016-11-15 2021-10-06 協同乳業株式会社 フソバクテリウム・ヌクレアタムの菌株識別方法
US20240382458A1 (en) * 2021-09-17 2024-11-21 National University Corporation Tokai National Higher Education And Research System Method for detecting bacteria of genus fusobacterium in order to diagnose endometriosis

Also Published As

Publication number Publication date
JPWO2024053642A1 (https=) 2024-03-14
WO2024053642A1 (ja) 2024-03-14
EP4553165A1 (en) 2025-05-14

Similar Documents

Publication Publication Date Title
RU2673733C2 (ru) Способ амплификации днк на основе внедрения в цепь
KR102470985B1 (ko) 브루셀라균 감별을 위한 단일염기다형성 마커 및 이를 검출하기 위한 프라이머 세트
JP2015536676A (ja) 糞便サンプル中のヘリコバクターピロリdnaを検出するための方法
US10174386B2 (en) Method of quantitatively analyzing microorganism targeting rRNA
CN114790490B (zh) 一种可区分牛种和羊种布鲁氏菌的分子标记和检测方法
US20110287965A1 (en) Methods and compositions to detect clostridium difficile
EP4130272A1 (en) Primer set and probe for detecting klebsiella bacteria
KR101509071B1 (ko) 마이크로시스티스속 균주의 유전자 증폭용 프라이머 및 이를 이용한 마이크로시스티스속 균주의 탐지방법
KR101288035B1 (ko) 브루셀라균 종별 감별용 프라이머 세트
CN1724686B (zh) 用于检测肺炎支原体的靶序列和试剂盒
EP3115466A1 (en) Listeria monocytogenes clonogrouping and assessment of infectivity
CN117467781B (zh) 与布鲁氏菌复方新诺明耐药性相关的snp标记、pcr引物对和探针对、试剂盒及其应用
JP2019062815A (ja) 虫由来dnaの断片を増幅するためのプライマーセット、及び、それを用いた虫種の同定方法
KR101395938B1 (ko) 장수풍뎅이 질병 유발 세균 진단용 프라이머 및 그 진단방법
KR102860454B1 (ko) 마이코플라스마 갈리셉티쿰 검출용 프라이머 세트 및 이를 이용한 검출방법
CN119365607A (zh) 具核梭杆菌的检测方法和具核梭杆菌具核亚种的检测方法
EP3438280B1 (en) Haemoplasma detection method
CN110894532A (zh) 细菌性败血症(fbs)的raa恒温荧光检测方法及试剂
KR102655121B1 (ko) 락토바실러스 사케이 k040706 균주 검출용 프라이머 세트 및 이의 용도
KR102502129B1 (ko) Pna 프로브를 이용한 돼지 흉막폐렴균의 혈청형 진단방법 및 키트
KR102520374B1 (ko) Pna 프로브를 이용한 돼지 흉막폐렴균의 혈청형 진단방법 및 키트
AU2006226873A1 (en) Nucleic acid detection
KR102291584B1 (ko) 반려동물 및 가축의 질병진단을 위한 분자진단 방법
KR102359838B1 (ko) 요네병균의 서브타입 판별용 바이오마커 및 이의 용도
EP3822370A1 (en) Method of determining the presence of a hyper-virulent clostridioides difficile strain of the b1/nap1/027 group in a sample

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination