CN1190136C - Biologic pesticide and preparation method thereof - Google Patents
Biologic pesticide and preparation method thereof Download PDFInfo
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- CN1190136C CN1190136C CNB02156521XA CN02156521A CN1190136C CN 1190136 C CN1190136 C CN 1190136C CN B02156521X A CNB02156521X A CN B02156521XA CN 02156521 A CN02156521 A CN 02156521A CN 1190136 C CN1190136 C CN 1190136C
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- biological insecticides
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Abstract
The present invention discloses biological insecticide and a preparation method thereof. The insecticide comprises insecticidal microorganisms, microflow membranes wrapping the microorganisms, and carries, wherein the microorganisms are selected from fungi, bacteria, actinomycetes, viruses, eelworms and microsporidians.
Description
Technical field
The present invention relates to a kind of biological insecticides and preparation method thereof.
Background technology
In order to guarantee the shelf life of biopesticide, its active component is general to adopt the brood body that is in resting state, as conidium, blastopore, perhaps manually impels the trophosome of dormancy, as dried mycelia etc.Active component is subjected to environmental factor such as moisture, temperature, ultraviolet ray in the survival in field and in the interaction of interacting goals thing (being target), and the restriction of the character at both interfaces etc.
Arthropods body surface or plant surface all have the wax coat that prevents moisture loss, in the very long coevolution process of natural world, the natural enemy microbe of these arthropodss or plant also develops the spore that the surface coverage hydrophobe as infecting body, strengthens the affinity to target.But be the biopesticide of active component with living microorganism or its biological metabolic product at present, mainly continue to use the formulation technology of chemical pesticide.Be mainly a barrel water distribution agent (now with the current), wetting powder.All contain surfactant in bucket water distribution suspending agent and the wetting powder, guarantee in the water to spray evenly so that active component is suspended in equably.But surfactant have a negative effect: (1) changed active component for example fungi protect Surface Physical character, make it become hydrophilicly, reduced and the binding ability of target (promptly because arthropods body surface or plant surface all have hydrophobic wax coat) by hydrophobic; (2) influence active component and render a service, reduced the germination rate of fungal spore; (3) after active component entered water body in large, owing to lose the support of suspending agent and stabilizing agent in the former formulation, gravitate can sink under water, thereby arthropods and plant movable to the water surface or that float are ineffective.
Types such as present existing pulvis and finish are are mainly prevented and treated blade face insect and soil insect, adopt pulvis is mixed the method for imbedding with inert carriers such as seed, fertilizer or soil; The control forestry pest is based on direct sprinkling pulvis; The control locust adopts finish to carry out ultra low volume spray more.
Summary of the invention
In order to overcome the above-mentioned defective of prior art, the invention provides a kind of new biopesticide formulation.
The objective of the invention is by the surface of microbial pesticide active component parcel one deck hydrophobic, have protection active and promote the microfluidic film of preventive effect that a kind of novel biopesticide that keeps microbial activity is provided.
One embodiment of the invention provide a kind of biological insecticides, comprise the microfluidic film of living microorganism and the described living microorganism of parcel.Described biological insecticides also can comprise and are used for the carrier that mixes with described living microorganism.Described living microorganism is selected from fungi, bacterium, actinomycetes, virus, nematode and microsporidian.Be preferably selected from fungi, bacterium, actinomycetes, virus.More preferably be selected from fungi, bacterium, actinomycetes, or be selected from fungi, bacterium; Be selected from fungi, actinomycetes.The best is selected from fungi; Particularly be selected from insect pathogenic fungus, green muscardine fungus for example, for example IBCCM321.93 (this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on December 12nd, 2002, and preserving number is CGMCC No.0853), white muscardine fungi, paecilomycerol, thorn dish spore bacterium.
Used microfluidic film is by tool flowability under not hydrophilic, the normal temperature in the biological insecticides of the present invention, and is nontoxic or reduce its effect and can constitute with the affine naturally material of arthropods body surface or plant surface wax coat to described living microorganism.Described material can be selected from peanut oil, sunflower oil, Semen Benincasae oil, soybean oil, sesame oil or corn wet goods vegetable oil, perhaps inert mineral oil, for example paraffin oil, the senior fatty hydrocarbon ils of methyl naphthalene.Its effect is to form tempered oil film and can lure cockroach at spore surface.The preferred edible oil that uses in the shelf-life.The effect of described microfluidic film is the wetting action that (1) utilizes lipid hydrophobic membrane and arthropods or plant surface wax coat, strengthens under capillary effect or gives the ability that active component combines with target; (2) lipid film material itself has the effect that reduces exchange of moisture.Utilize the film of the different-thickness that process forms naturally that the active component of dormancy is recovered at different time, prolong the desinsection time, the thickness of described film is normal distribution, different according to active component itself size and surface characteristic, coated fertilizer, operating condition, equipment, the thickness of film is also different, is generally the 1-10 micron; (3) by the membrane material self character or add protectant, as ultra-violet absorber, pH buffer, alleviate or prevent that extraneous undesirable element from influencing active component, extends the expiration date; (4) for some particular dosage form, adding man-hour can be with active component with the carrier coating, improves the water repellency of composite, make its float the water surface and accumulate in water plants around be added to the touch opportunity of target, improve control efficiency.
The carrier proportion that mixes with active component is less than 2.5 greater than 0.1, and granularity is 0.02-10mm, on the institute's water surface that can float after carrier itself or the processing.Described carrier comprises vegetalitas carrier, mineral carrier, organic synthesis thing and soluble carrier.The vegetalitas carrier can be selected from: sawdust, plant species shell powder, maize cob meal, cork powder, the peat composed of rotten mosses, active carbon, lignin, furfural dregs, plant straw powder.The mineral carrier can be selected from diatomite, attapulgite, bentonite, sepiolite, zeolite, float stone, vermiculite, perlite, kaolin, white carbon, pumice, flyash.The organic synthesis thing is optional from foaming polyvinyl chloride, polystyrene, sodium alginate.Soluble carrier can be selected from gypsum, chemical fertilizer, for example urea, ammonium nitrate, potassium sulphate, dipotassium hydrogen phosphate etc.
Also can add uv-protector, for example cinoxate in the biological insecticides of the present invention.
The method for preparing biological insecticides of the present invention comprises puts into mixer with microorganism, and high speed dispersion limit, limit sprays into vegetable oil, forms oil film at spore surface; Add carrier then, be changed to stirring at low speed and spray into vegetable oil, to be mixed evenly after, above-mentioned material is made the particle of diameter 0.3-0.5 millimeter with comminutor.
The another kind of method of preparation biological insecticides of the present invention is that the dried mycelium of fungi is put into fluid bed coating machine (supplier joins the coater annex for Aeromatic-Fielder AG STREA-1), after the gas speed of adjusting fluid bed makes material suspended mixing, spray into vegetable oil or inert oil with end spray method, for example paraffin oil forms tunicle on dried mycelium powder surface.
The another kind of preparation method of biological insecticides of the present invention is concentrated into dry matter content with microbial fermentation solution to reach more than 10%, zymotic fluid is sprayed into attapulgite (fineness 300 orders, the density 2.2g/cm that loads identical weight in advance
3, apparent density 0.14-0.19g/cm
3) fluid-bed dryer (supplier is Aeromatic-Fielder AG STREA-1) in, 90 ℃ are dried to material moisture less than 5%; At the cooling section of fluid-bed dryer, spray into 1: 1 vegetable oil and inert oil in the ratio of 1-6% (v/w), for example the mixture of paraffin forms oil film on the material surface.
Embodiment
Embodiment 1 green muscardine fungus (Metarhizium spp.)
The spore of 1 kilogram of Metarhizium anisopliae (Metarhizium anisopliae) is put into mixer (supplier NARA MACHINERY CO.LTD NMG-15), high speed dispersion limit, limit sprays into the 0.05 liter of corn oil that contains 10% cinoxate, forms the oil film of 3-3.5 micron thickness at spore surface; Add 99 kilograms of diatomite (fineness 300 orders, density 2.0g/cm then
3, apparent density 0.1-0.2g/cm
3), be changed to stirring at low speed then and spray into 9.55 liters of corn oils, to be mixed evenly after, above-mentioned material is made the particle of diameter 0.3-0.5 millimeter with comminutor.The product of making can be separately or with after carrier of the present invention mixes, and manually spreads fertilizer over the fields control paddy field insect, is sprinkling upon ground or the control soil insect or the insect of soil life stage is arranged of placing into the soil.
Embodiment 2 white muscardine fungis (Beauveria spp.) preparation
With 20 kilograms of fineness is that the dried mycelium of 20 purpose muscardines (Beauveria brongniatii) is put into fluid bed coating machine (supplier joins the coater annex for Aeromatic-Fielder AG STREA-1), after the gas speed of adjusting fluid bed makes material suspended mixing, spray into 1 liter of paraffin oil with end spray method, form tunicle on dried mycelium powder surface.The good product of coating can be separately or with after carrier of the present invention mixes, and manually spreads fertilizer over the fields control paddy field insect, is sprinkling upon ground or the control soil insect or the insect of soil life stage is arranged of placing into the soil.
Embodiment 3 white muscardine fungis (Beauveria spp.) preparation-desinsection fertilising complexing agent
In roller coating machine (supplier: 10 kilograms in coated slow-release type urea putting into particle diameter 6-8 millimeter the BY400 of Tianxiang Jiantai Pharmaceutical Machinery Co., Ltd., Shanghai dressing machine), add 60 gram beauveria bassiana (Beauveria bassiana) conidial powder, rolling limit, limit sprays into 20 milliliters of soybean oils, be rolled into all spores powder parcel chemical fertilizer surface, because the lipophile of beauveria bassiana spore, vegetable oil-soybean oil forms coating at spore surface.Add the gesso of 140 grams, 400 order fineness then, rolling limit, limit sprays into 64 ml waters, solidifies owing to plaster of paris chance water is reduced to gypsum, shows the formation dressing at sustained release fertilizer.Make Tian Houhui disintegration gradually when the granule of making is sprinkled into, discharge the beauveria bassiana spore and the fertilizer nutrient of oil film parcel, the purpose that reaches not only desinsection but also apply fertilizer.
Embodiment 4 paecilomycerols (Paecilomyces spp.) preparation
After the solid culture of 5 kilograms of powder Paecilomyces variotis (Paecilomyces) is crushed to 20 orders (particle diameter 0.85mm), put into mixer, spray into 0.25 liter of Semen Benincasae oil and two wires oil (intermediate product of oil plant is the by product of kerosene) mixture while stirring, fully stir by mixing in 2: 1; Add 40 kilograms, length straw crushed material (apparent density 0.1-0.18g/cm then less than 10mm
3, apparent density is the bulk density after the carrier processing), continue to spray into 2.45 liters of Semen Benincasae oils while stirring to mixing.The product of making can be separately or with after carrier of the present invention mixes, and manually spreads fertilizer over the fields control paddy field insect, is sprinkling upon ground or the control soil insect or the insect of soil life stage is arranged of placing into the soil.
Embodiment 5 thorn dish spore bacterium (Colletotricum spp.) preparation-biological weed killers
Orange thorn dish spore bacterium (Colletotricum gleosporioides) zymotic fluid is concentrated into dry matter content and reaches more than 10%, zymotic fluid is sprayed into attapulgite (fineness 300 orders, the density 2.2g/cm that loads identical weight in advance
3, apparent density 0.14-0.19g/cm
3) fluid-bed dryer (supplier is Aeromatic-Fielder AG STREA-1) in, 120 ℃ are dried to material moisture less than 5%; At the cooling section of fluid-bed dryer, spray into 1: 1 soybean oil and paraffin oil in the ratio of 1-6% (v/w), form oil film on the material surface.The good product of coating can be separately or with after carrier of the present invention mixes, and manually spreads fertilizer over the fields control paddy field insect, is sprinkling upon ground or the control soil insect or the insect of soil life stage is arranged of placing into the soil.
Claims (18)
1. biological insecticides, it is characterized in that, the microfluidic film that comprises microorganism and the described microorganism of parcel, described microfluidic film is by tool flowability under not hydrophilic, the normal temperature, nontoxic or reduce its effect and can constitute with the affine naturally material of arthropods body surface or plant surface wax coat to described microorganism, the material that constitutes the microfluidic film can be selected from peanut oil, sunflower oil, Semen Benincasae oil, soybean oil, sesame oil, corn oil, paraffin oil, the senior fatty hydrocarbon ils of methyl naphthalene.
2. biological insecticides as claimed in claim 1 is characterized in that described microorganism is selected from fungi, bacterium, actinomycetes, virus, nematode, microsporidian.
3. biological insecticides as claimed in claim 1 is characterized in that described microorganism is selected from fungi, bacterium, actinomycetes, virus.
4. biological insecticides as claimed in claim 1 is characterized in that described microorganism is selected from fungi, bacterium, actinomycetes.
5. biological insecticides as claimed in claim 1 is characterized in that described microorganism is selected from fungi, bacterium.
6. biological insecticides as claimed in claim 1 is characterized in that described microorganism is selected from fungi, actinomycetes.
7. biological insecticides as claimed in claim 1 is characterized in that described microorganism is selected from fungi.
8. biological insecticides as claimed in claim 1 is characterized in that described microorganism is selected from insect pathogenic fungus.
9. as the arbitrary described biological insecticides of claim 1-8, it is characterized in that, also comprise being used for the carrier that mixes with described microorganism, described carrier can be selected from sawdust, plant species shell powder, maize cob meal, cork powder, the peat composed of rotten mosses, active carbon, lignin, furfural dregs, plant straw powder, diatomite, attapulgite, bentonite, sepiolite, zeolite, float stone, vermiculite, perlite, kaolin, white carbon, pumice, flyash, foamed polyvinyl chloride, polystyrene, sodium alginate, gypsum, chemical fertilizer.
10. biological insecticides as claimed in claim 9 is characterized in that described chemical fertilizer is selected from urea, ammonium nitrate, potassium sulphate, dipotassium hydrogen phosphate.
11. as the arbitrary described biological insecticides of claim 1-8, it is characterized in that, also can comprise uv-protector.
12. biological insecticides as claimed in claim 9 is characterized in that, also can comprise uv-protector.
13. biological insecticides as claimed in claim 10 is characterized in that, also can comprise uv-protector.
14. biological insecticides as claimed in claim 13 is characterized in that, the described carrier proportion that mixes with active component is less than 2.5 greater than 0.1, and granularity is 0.02-10mm, on the institute's water surface that can float after carrier itself or the processing.
15. biological insecticides as claimed in claim 14 is characterized in that, described carrier comprises vegetalitas carrier, mineral carrier, organic synthesis thing and soluble carrier; Described vegetalitas carrier can be selected from: sawdust, plant species shell powder, maize cob meal, cork powder, the peat composed of rotten mosses, active carbon, lignin, furfural dregs, plant straw powder; Described mineral carrier can be selected from diatomite, attapulgite, bentonite, sepiolite, zeolite, float stone, vermiculite, perlite, kaolin, white carbon, pumice, flyash.The organic synthesis thing is optional from foaming polyvinyl chloride, polystyrene, sodium alginate; Described soluble carrier can be selected from gypsum, urea, ammonium nitrate, potassium sulphate, dipotassium hydrogen phosphate.
16. one kind prepares the method for biological insecticides according to claim 1, it is characterized in that, comprises microorganism is put into mixer, high speed dispersion limit, limit sprays into vegetable oil, forms oil film on the microorganism surface; Add carrier then, be changed to stirring at low speed and spray into vegetable oil, to be mixed evenly after, above-mentioned material is made the particle of diameter 0.3-0.5 millimeter with comminutor.
17. one kind prepares the method for biological insecticides according to claim 1, it is characterized in that, the dried mycelium of fungi is put into the fluid bed coating machine, after the gas speed of adjusting fluid bed makes material suspended mixing, spray into vegetable oil or paraffin oil with end spray method, form tunicle on dried mycelium powder surface.
18. one kind prepares the method for biological insecticides according to claim 1, it is characterized in that, microbial fermentation solution is concentrated into dry matter content to be reached more than 10%, zymotic fluid is sprayed in the fluid-bed dryer of the attapulgite that loads identical weight in advance, the attapulgite fineness is 300 orders, and density is 2.2g/cm
3, apparent density is 0.14-0.19g/cm
3, 90 ℃ are dried to material moisture less than 5%; At the cooling section of fluid-bed dryer, spray into 1: 1 the vegetable oil and the mixture of paraffin oil or paraffin in the ratio of 1-6% (v/w), form oil film on the material surface.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB02156521XA CN1190136C (en) | 2002-12-16 | 2002-12-16 | Biologic pesticide and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB02156521XA CN1190136C (en) | 2002-12-16 | 2002-12-16 | Biologic pesticide and preparation method thereof |
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| CN1415212A CN1415212A (en) | 2003-05-07 |
| CN1190136C true CN1190136C (en) | 2005-02-23 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103451100A (en) * | 2013-08-28 | 2013-12-18 | 河北晟普生物科技有限公司 | Locust microspore chorthippus strain and applications thereof |
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| CN100381557C (en) * | 2004-08-26 | 2008-04-16 | 华南理工大学 | Cultivating method for scarab green muscardine fungus solid state fermentation |
| CN101564047B (en) * | 2009-05-27 | 2012-02-15 | 重庆大学 | Disinsection fungi emulsifiable powder |
| CN102199589B (en) * | 2010-12-27 | 2015-01-14 | 北京师范大学 | Immobilized microspheres for remediation of petroleum contaminated soil, preparation method thereof and application thereof |
| CN104041519A (en) * | 2013-03-13 | 2014-09-17 | 河北农业大学 | Streptomyces roseoflavus microbial fungicide particulate agent |
| CN103548798A (en) * | 2013-10-29 | 2014-02-05 | 武汉大学 | Method and device for microbial pest control based on light luring |
| CN105076216A (en) * | 2014-05-20 | 2015-11-25 | 段一皋 | Microbial combined insecticide and preparation method thereof |
| CN104642394B (en) * | 2015-02-11 | 2017-06-20 | 中国农业科学院植物保护研究所 | Prevent and treat green muscardine fungus granule of thrips and preparation method and application |
| CN105594750A (en) * | 2015-09-14 | 2016-05-25 | 北京晨奥润泽科技股份有限公司 | Microbial insecticide and preparation method thereof |
| CN105360167A (en) * | 2015-09-26 | 2016-03-02 | 黄山学院 | Density-board-based slow-released biological prevention and control agent and preparing method and application thereof |
| CN105230665A (en) * | 2015-09-26 | 2016-01-13 | 黄山学院 | Wax paper based sustain-released bio-control agent, preparation method and applications thereof |
| CN105230666A (en) * | 2015-09-26 | 2016-01-13 | 黄山学院 | Sustain-released bio-control agent, preparation method and applications thereof |
| CN106747804A (en) * | 2016-12-15 | 2017-05-31 | 中国热带农业科学院环境与植物保护研究所 | A kind of Methylotrophic gemma bacillus agent and preparation method thereof |
| CN107162717A (en) * | 2017-05-11 | 2017-09-15 | 北京中农富源生物工程技术有限公司 | A kind of anthropogenic soil efficiently repairs complex micro organism fungicide |
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2002
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103451100A (en) * | 2013-08-28 | 2013-12-18 | 河北晟普生物科技有限公司 | Locust microspore chorthippus strain and applications thereof |
| CN103451100B (en) * | 2013-08-28 | 2015-06-17 | 河北晟普生物科技有限公司 | Locust microspore chorthippus strain and applications thereof |
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| CN1415212A (en) | 2003-05-07 |
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