CN105230666A - Sustain-released bio-control agent, preparation method and applications thereof - Google Patents

Sustain-released bio-control agent, preparation method and applications thereof Download PDF

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Publication number
CN105230666A
CN105230666A CN201510628997.XA CN201510628997A CN105230666A CN 105230666 A CN105230666 A CN 105230666A CN 201510628997 A CN201510628997 A CN 201510628997A CN 105230666 A CN105230666 A CN 105230666A
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China
Prior art keywords
control agent
biological control
slow release
release type
type biological
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Pending
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CN201510628997.XA
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Chinese (zh)
Inventor
李丰伯
李晓毓
崔谱
方乐金
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Huangshan University
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Huangshan University
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Priority to CN201510628997.XA priority Critical patent/CN105230666A/en
Publication of CN105230666A publication Critical patent/CN105230666A/en
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Abstract

The invention discloses a sustain-released bio-control agent, which comprises the following raw materials: biodegradable solid waste, inoculated fungal spore powder, and a binding agent. The invention also discloses a preparation method of the sustain-released bio-control agent. The preparation method comprises the following steps: step one, grinding biodegradable solid waste into fragments with a particle size of 2 to 4 mm, and then evenly mixing the fragments with inoculated fungal spore powder to obtain a material (a); step two, artificially binding the material (a) obtained in the step one by the binding agent to prepare particles; step three, and painting colors on the surface of the particles to obtain the sustain-released bio-control agent. The invention also relates an application of the sustain-released bio-control agent, which can be evenly sprayed into farmland or woodland through human, machinery, display shell, or aviation. The preparation method has the advantage that the biodegradable waste is converted into valuable resources. Moreover, the spore powder can be slowly released, and thus the bio-control effect can last for a long time.

Description

A kind of slow release type biological control agent and its preparation method and application
Technical field
The present invention relates to biological control plant disease field, particularly relate to a kind of slow release type biological control agent and its preparation method and application.
Background technology
Crops and plant pest can make plant leaf blade generation variable color, downright bad, deformity, wilt, perforation, the symptom such as caducous, serious constrains crops, economic crops, the high yield of medicinal plant, stable yields and high-quality, Prevention Technique conventional at present mainly contains the anti-therapy of chemical method and measure of biotic control increasingly mature in recent years, but along with people are to pollution-free, the raising day by day of pollution-free green food cry, owing to there is the accumulation of agricultural chemicals in chemical control method, contaminated environment, the defect such as the harmful and health requirements of people can not be met, biological control mainly realizes the control of damage by disease and insect by the allelopathy between biology, progressively replacing traditional chemical control method and becoming a kind of important extermination of disease and insect pest means, but measure of biotic control ubiquity biocontrol microorganisms growth time is short, be subject to the unfavorable factors such as external environment condition variable effect, seriously constrain its control agriculture, the effect of forestry pests & diseases, so be difficult to the continuation realizing the extermination of disease and insect pest.Therefore, while ensureing biological control effect stability, how realize the continuation of the extermination of disease and insect pest, meet the extermination of disease and insect pest in plant growing cycle for most important biological prevention.
Along with human industry's civilization, the raising of agricultural civilization and daily life level, the solid waste kind that industrial or agricultural activity and everyday life produce and quantity unprecedentedly increase, and along with the progress of science and technology and being rooted in the hearts of the people of environmental protection concept, biodegradable material has become the chief component of all kinds of solid waste, although all kinds of biodegradable solid refuse can disintegrate in the environment, but easily can cause visual pollution and landscape pollution owing to discharging arbitrarily and being scattered, therefore, current processing mode is still concentrated and is cleared, sanitary landfills or burning disposal are done in unification.Landfill disposal is mainly based on the fermentation of anaerobe, therefore poisonous and hazardous percolate can be produced, percolate can under the seepage effect of earth's surface direct polluted underground water, also can polluted surface water further if disposal options is improper, and the burning process of solid waste can produce as toxic and harmful contaminated airs such as bioxin, if and biodegradable solid refuse aerobic degradation in natural environment, then product is mostly as CO 2, H 2the Small molecular that O, ethanol etc. are nontoxic, limited to the pollution capacity of environment.
According to the inventors knowledge, there is no document or patent at present and openly report that utilizing biodegradable solid refuse to wrap up fungal spore powder implants farmland, forest land for persistent prevention and elimination of disease and pests.
Summary of the invention
For solving background technology Problems existing, a kind of slow release type biological control agent that the present invention proposes.
The present invention proposes a kind of slow release type biological control agent, and its raw material comprises by weight: fungal spore powder 20-40 part of biodegradable solid refuse 80-120 part, inoculation, adhesion agent 10-30 part.
Preferably, its raw material comprises by weight: fungal spore powder 25-35 part of biodegradable solid refuse 85-115 part, inoculation, adhesion agent 15-25 part.
Preferably, described biodegradable solid refuse is one or more compositions in polyurethane foam material, improvement PE material, agricultural stalk, agricultural film, disposable snack box and waste paper;
Preferably, the fungal spore powder of described inoculation be that bacillus thuringiensis, Ma Lian are mould, beauveria bassiana, chafer green reins bacterium and rootworm pest mould in one or more compositions.
Preferably, described adhesion agent is one or more compositions in polyesteramide, PHA and polylactide type biodegradation type PUR.
The preparation method of a kind of slow release type biological control agent that the present invention proposes, comprises the steps:
S1, biodegradable solid refuse is ground into the fragment that particle diameter is 2-4mm, then mixes with the fungal spore powder of inoculation and obtain material a;
S2, the material a obtained by S1 adopt adhesion agent to be manually prepared into granular material;
S3, color is coated on granular material surface obtain slow release type biological control agent.
Preferably, in S2, the particle diameter of described granular material is 0.05-2.00cm.
Preferably, in S3, described color is red, blue or yellow.
The application of a kind of slow release type biological control agent that the present invention proposes, slow release type biological control agent is for manually to throw solarization, and machinery is shed, and the mode that display shell or aviation are shed evenly implants farmland or forest land.
Preferably, the release duration of the fungal spore powder of the inoculation in described slow release type biological control agent is 1-15 month.
Beneficial effect of the present invention comprises:
1, the method utilizes biodegradable solid waste to wrap up the slow releasing that entomogenous fungi conidial powder implants farmland, forest land achieves biocontrol fungi, has filled up the blank of measure of biotic control slow releasing technology to a certain extent;
2, the conidial powder of biodegradable solid refuse to biological and ecological methods to prevent plant disease, pests, and erosion agent is utilized to wrap up, lapping itself is to spore nonhazardous effect, and conidial powder can be slowly released along with the last decomposition of lapping and enters in external environment, the persistence of agricultural field damage by disease and insect biological control can be realized;
3, conidial powder light weight, adhesion are strong, just can be elegant under the effect of the external conditions such as wind-force, cross-ventilation, plant swing in external environment once be released into, and be attached to more phytopathy affected part, so be more conducive to this biocontrol agent to play biological control effect uniformly;
4, this slow release type biological control agent with biodegradable solid refuse for lapping, effectively achieve the resource of all kinds of biodegradable solid refuse, decrease the cost that this part solid waste centrally disposes, solve the percolate that produces in this fraction solids refuse conventional process mode or the secondary environmental pollution problem caused by all kinds of air pollution factor simultaneously;
5, biocontrol agent particle exterior surface smears various painted color, can effectively preventing birds and animal eat;
6, this slow release type biological control agent can be wrapped up according to the single conidial powder of feature selecting of agricultural field damage by disease and insect or multiple conidial powder, to realize the specific aim of biological control;
7, the outer covering material of slow release type biological control agent can produce N, P, S, inorganic salt and other material in the process of slowly degraded, therefore its degradation process can provide certain nutritive element for the growth of plant, can reduce the amount of application of all kinds of chemical fertilizer in a way.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail; should understand; embodiment is only for illustration of the present invention, instead of for limiting the present invention, any amendment, equivalent replacement etc. made on basis of the present invention is all in protection scope of the present invention.
Embodiment 1
A kind of slow release type biological control agent that the present invention proposes, its raw material comprises by weight: polyurethane foam material 100 parts, bacillus thuringiensis 30 parts, polyesteramide 20 parts.
The preparation method of a kind of slow release type biological control agent that the present invention proposes, comprises the steps:
S1, by proportioning, polyurethane foam material is ground into the fragment that particle diameter is 2-4mm, then mixes with bacillus thuringiensis and obtain material a;
S2, the material a obtained by S1 adopt polyesteramide to be manually prepared into the granular material that particle diameter is 0.05-2.00cm;
S3, color is coated on granular material surface obtain slow release type biological control agent, color is red.
The application of a kind of slow release type biological control agent that the present invention proposes, slow release type biological control agent is that the artificial mode of shining of throwing evenly implants farmland or forest land, and the release duration of the fungal spore powder of the inoculation in described slow release type biological control agent is 8 months.
Embodiment 2
A kind of slow release type biological control agent that the present invention proposes, its raw material comprises by weight: improvement PE material 80 parts, mould 40 parts of Ma Lian, PHA 10 parts.
The preparation method of a kind of slow release type biological control agent that the present invention proposes, comprises the steps:
S1, the fragment being become by improvement PE material disintegrating particle diameter to be 2-4mm, then mix mould with Ma Lian obtains material a;
S2, the material a obtained by S1 adopt PHA to be manually prepared into the granular material that particle diameter is 0.05-2.00cm;
S3, color is coated on granular material surface obtain slow release type biological control agent, color is blue.
The application of a kind of slow release type biological control agent that the present invention proposes, slow release type biological control agent is that the mode that machinery is shed evenly implants farmland or forest land, and the release duration of the fungal spore powder of the inoculation in described slow release type biological control agent is 1 month.
Embodiment 3
A kind of slow release type biological control agent that the present invention proposes, its raw material comprises by weight: agricultural stalk 120 parts, beauveria bassiana 20 parts, polylactide type biodegradation type PUR 30 parts.
The preparation method of a kind of slow release type biological control agent that the present invention proposes, comprises the steps:
S1, agricultural stalk is ground into the fragment that particle diameter is 2mm, then mixes with the beauveria bassiana of inoculation and obtain material a;
S2, the material a obtained by S1 adopt polylactide type biodegradation type PUR to be manually prepared into the granular material that particle diameter is 2.00cm;
S3, color is coated on granular material surface obtain slow release type biological control agent, color is yellow.
The application of a kind of slow release type biological control agent that the present invention proposes, slow release type biological control agent is that the mode of display shell evenly implants farmland or forest land, and the release duration of the fungal spore powder of the inoculation in described slow release type biological control agent is 15 months.
The above; be only the present invention's preferably embodiment; but protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to according to technical scheme of the present invention and inventive concept thereof and replace or change, all should be encompassed within protection scope of the present invention.

Claims (10)

1. a slow release type biological control agent, is characterized in that, its raw material comprises by weight: fungal spore powder 20-40 part of biodegradable solid refuse 80-120 part, inoculation, adhesion agent 10-30 part.
2. slow release type biological control agent according to claim 1, is characterized in that, its raw material comprises by weight: fungal spore powder 25-35 part of biodegradable solid refuse 85-115 part, inoculation, adhesion agent 15-25 part.
3. slow release type biological control agent according to claim 1 and 2, it is characterized in that, described biodegradable solid refuse is one or more compositions in polyurethane foam material, improvement PE material, agricultural stalk, agricultural film, disposable snack box and waste paper.
4. the slow release type biological control agent according to any one of claim 1-3, it is characterized in that, the fungal spore powder of described inoculation is that bacillus thuringiensis, Ma Lian are mould, beauveria bassiana, chafer green reins bacterium and rootworm pest mould in one or more compositions.
5. the slow release type biological control agent according to any one of claim 1-4, is characterized in that, described adhesion agent is one or more compositions in polyesteramide, PHA and polylactide type biodegradation type PUR.
6. a preparation method for the slow release type biological control agent according to any one of claim 1-5, is characterized in that, comprise the steps:
S1, biodegradable solid refuse is ground into the fragment that particle diameter is 2-4mm, then mixes with the fungal spore powder of inoculation and obtain material a;
S2, the material a obtained by S1 adopt adhesion agent to be manually prepared into granular material;
S3, color is coated on granular material surface obtain slow release type biological control agent.
7. the preparation method of slow release type biological control agent according to claim 6, is characterized in that, in S2, the particle diameter of described granular material is 0.05-2.00cm.
8. the preparation method of the slow release type biological control agent according to claim 6 or 7, is characterized in that, in S3, described color is red, blue or yellow.
9. an application for the slow release type biological control agent according to any one of claim 1-5, is characterized in that, slow release type biological control agent is for manually to throw solarization, and machinery is shed, and the mode that display shell or aviation are shed evenly implants farmland or forest land.
10. the application of slow release type biological control agent according to claim 9, is characterized in that, the release duration of the fungal spore powder of the inoculation in described slow release type biological control agent is 1-15 month.
CN201510628997.XA 2015-09-26 2015-09-26 Sustain-released bio-control agent, preparation method and applications thereof Pending CN105230666A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106106441A (en) * 2016-06-05 2016-11-16 河南科技学院 A kind of slow release type biological control agent and its preparation method and application
CN106316659A (en) * 2016-07-28 2017-01-11 安徽尚善生物科技股份有限公司 Insect-resistant fertilizer filler with purple cabbage waste residues as main material and preparation method thereof
CN109042640A (en) * 2018-07-19 2018-12-21 青岛正利纸业有限公司 A kind of slow-release carrier granular and preparation method thereof
CN109762744A (en) * 2019-03-01 2019-05-17 中国科学院昆明植物研究所 Fungal bacterial strain and its cultural method and purposes for degradable polyurethane plastics

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4925663A (en) * 1987-12-31 1990-05-15 University Of Florida Biological control of imported fire ants with a fungal pathogen
CN1415212A (en) * 2002-12-16 2003-05-07 高松 Biologic pesticide and preparation method thereof
CN101411338A (en) * 2008-12-03 2009-04-22 安徽农业大学 Beauveria bassiana granular formulation and preparation method thereof
CN101502271A (en) * 2009-03-25 2009-08-12 中国农业科学院植物保护研究所 Method for producing fungal granular formulation for preventing and treating pest
CN103004888A (en) * 2012-05-16 2013-04-03 中国农业科学院植物保护研究所 Metarrhizium anisopliae granular preparation and preparation method thereof
CN104642394A (en) * 2015-02-11 2015-05-27 中国农业科学院植物保护研究所 Green muscardine fungus granules for controlling thrips as well as preparation method and application thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4925663A (en) * 1987-12-31 1990-05-15 University Of Florida Biological control of imported fire ants with a fungal pathogen
CN1415212A (en) * 2002-12-16 2003-05-07 高松 Biologic pesticide and preparation method thereof
CN101411338A (en) * 2008-12-03 2009-04-22 安徽农业大学 Beauveria bassiana granular formulation and preparation method thereof
CN101502271A (en) * 2009-03-25 2009-08-12 中国农业科学院植物保护研究所 Method for producing fungal granular formulation for preventing and treating pest
CN103004888A (en) * 2012-05-16 2013-04-03 中国农业科学院植物保护研究所 Metarrhizium anisopliae granular preparation and preparation method thereof
CN104642394A (en) * 2015-02-11 2015-05-27 中国农业科学院植物保护研究所 Green muscardine fungus granules for controlling thrips as well as preparation method and application thereof

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106106441A (en) * 2016-06-05 2016-11-16 河南科技学院 A kind of slow release type biological control agent and its preparation method and application
CN106316659A (en) * 2016-07-28 2017-01-11 安徽尚善生物科技股份有限公司 Insect-resistant fertilizer filler with purple cabbage waste residues as main material and preparation method thereof
CN109042640A (en) * 2018-07-19 2018-12-21 青岛正利纸业有限公司 A kind of slow-release carrier granular and preparation method thereof
CN109762744A (en) * 2019-03-01 2019-05-17 中国科学院昆明植物研究所 Fungal bacterial strain and its cultural method and purposes for degradable polyurethane plastics

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Application publication date: 20160113