CN107162717A - A kind of anthropogenic soil efficiently repairs complex micro organism fungicide - Google Patents
A kind of anthropogenic soil efficiently repairs complex micro organism fungicide Download PDFInfo
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- CN107162717A CN107162717A CN201710327929.9A CN201710327929A CN107162717A CN 107162717 A CN107162717 A CN 107162717A CN 201710327929 A CN201710327929 A CN 201710327929A CN 107162717 A CN107162717 A CN 107162717A
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- micro organism
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
A kind of anthropogenic soil efficiently repairs complex micro organism fungicide, including the bacterium solution after complex micro organism fungicide fermentation, and composite adsorption carrier, and the mass ratio of bacterium solution and composite adsorption carrier is 0.01 0.2: 12, and viable count is 2.2 8.6 hundred million/gram;Described microbial bacterial agent includes bacillus megaterium, bacillus cereus, germ oligotrophy unit cell and pseudomonas putida;Described composite adsorption carrier includes agricultural crop straw, activated carbon and diatomite.The present invention is by bacillus megaterium, bacillus cereus, the bacterium solution of germ oligotrophy unit cell is combined, four kinds of bacterium are mutually promoted, the reparation to soil can be more efficiently completed, for pollution can be eliminated faster by heavy metal pollution, Organic chemical products contaminated soil, increase fertility of land, improve remediation efficiency.The composite adsorption carrier that the present invention is used overcomes the limitation of single carrier, extends the life-span of strain, improves the usage cycles of the strain of the present invention.
Description
Technical field
Field is repaired the present invention relates to edaphon technology, more particularly to a kind of anthropogenic soil efficiently repairs composite microbial
Thing microbial inoculum, preparation method and purposes.
Background technology
Modern agriculture application of substantial amounts of industrial chemical fertilizer, cause anthropogenic soil soil fertility to decline, and caking is serious, under crop yield
Drop, this phenomenon is referred to as " avoid ground phenomenon " in Japan, and the U.S., Britain, Canada claim " replanting disease ", and China often claims that " continuous cropping is asked
Topic ".
Moreover, as industrial pollution, water pollution, atmosphere pollution increasingly sharpen, agricultural cultivating soil declines in soil fertility
Meanwhile, also suffered from different degrees of chemical industry Organic Pollution (such as Organic pesticide residues, petroleum substance pollution) and
Heavy metal pollution.For heavy metal pollution, according to the investigation of agricultural sector of China, China accounts for by the land area of heavy metal pollution
The 64.8% of the Irrigation District of Sewage gross area, seriously threatens agricultural production and human health.
Microorganism remediation method is to improve the effective ways of soil productivity, has the advantages that many protrusions, secondary dirt is not produced
Dye, does not increase surrounding environment burden, cost is low, easy to operate, security is good, it is easy to manage.But, agricultural fertilizer in the market mesh
Preceding microbial bacterial agent strain is single, can only be used for specific soil, it is impossible to provide one kind and be widely used in various differences
The bacteria agent of soil, and different microbial inoculum is also difficult to play coordinative role.Existing microbial bacterial agent repair ability is limited.
The content of the invention
The present invention efficiently repairs composite microbial bacteria for above-mentioned deficiency of the prior art there is provided a kind of anthropogenic soil
Agent, preparation method and purposes.
Technical scheme is as follows.
A kind of anthropogenic soil efficiently repairs the bacterium solution after complex micro organism fungicide, including complex micro organism fungicide fermentation, with
And composite adsorption carrier, the mass ratio of bacterium solution and composite adsorption carrier is 0.01-0.2: 1-5, viable count be 2.2-8.6 hundred million/gram.
Wherein, described microbial bacterial agent include bacillus megaterium, bacillus cereus, germ oligotrophy unit cell with
And pseudomonas putida;Wherein, described bacillus megaterium, bacillus cereus, germ oligotrophy unit cell and stench
The bacterium solution mass ratio of pseudomonad is 1-3: 0.1-0.6: 0.2-0.5: 0.6-1.3;
Described composite adsorption carrier includes agricultural crop straw, activated carbon and diatomite;The particle diameter of the complex carrier
For 0.6-10mm;Wherein, described agricultural crop straw, activated carbon, diatomaceous mass ratio are 100-200: 1-5: 6-13.
Preferably, described bacillus megaterium, bacillus cereus, germ oligotrophy unit cell and Pseudomonas putida
The bacterium solution mass ratio of bacterium is 2: 0.6: 0.4: 0.9.
Preferably, described agricultural crop straw, activated carbon, diatomaceous mass ratio are 160: 4: 9.
Preferably, described agricultural crop straw is maize straw, wheat stalk or broomcorn straw.
The present invention further discloses the preparation method that above-mentioned anthropogenic soil efficiently repairs complex micro organism fungicide, bag
Include:
Step 1: the fermentation of strain
Bacillus megaterium is utilized into Zymolysis Equipment fermenting and producing, the first bacterium solution after being fermented;By waxy gemma bar
Bacterium utilizes Zymolysis Equipment fermenting and producing, the second bacterium solution after being fermented;Germ oligotrophy unit cell is sent out using Zymolysis Equipment
Ferment is produced, the 3rd bacterium solution after being fermented;Pseudomonas putida is utilized into Zymolysis Equipment fermenting and producing, the after being fermented
Four bacterium solutions;Four kinds of bacterium solutions of gained are mixed according to mass ratio 1-3: 0.1-0.6: 0.2-0.5: 0.6-1.3, mixed bacteria liquid is obtained;
Step 2: the preparation of composite adsorption carrier
By agricultural crop straw using being added to after crusher in crushing in mixer, activated carbon and diatomite are then added, then
Water is added, stirs and granulates, after drying, using high-temp steam sterilizing, vapor (steam) temperature is 126 DEG C, and steam pressure is
0.145MPa, time of contact is standby after 5min sterilizes, and the particle diameter of the complex carrier of preparation is 0.6-10mm;
Step 3: the preparation of microbial inoculum
The mixed bacteria liquid of step one is mixed with the composite adsorption carrier of step 2, while adding appropriate urea, nitric acid
Potassium, calcium superphosphate, borax and amino-oligosacchride;Wherein the consumption of urea, potassium nitrate, calcium superphosphate, borax and amino-oligosacchride is distinguished
It is 10-20 times, 10-15 times, 3-10 times, 1-5 times and 3-12 times of mixed bacteria liquid quality;
It is prepared by step 4, finished product
The mixed microbial inoculum of step 3 is crushed to particle diameter for 0.6-10mm, finished product is obtained.
Preferably, the specific surface area of described activated carbon is 600-1100m2/ g, is existing product;Described is diatomaceous
Specific surface area is 50-75m2/ g, porosity, up to more than 90%, is existing product.
Complex micro organism fungicide is efficiently repaired in soil remediation the present invention further discloses above-mentioned anthropogenic soil
Using described complex micro organism fungicide and conventional fertilizer application are mixed and imposed in upper soll layer soil, repairing efficiency is plant growth
Cycle.
The present invention achieves significantly technological progress.
In the present invention, described bacillus megaterium, bacillus cereus, germ oligotrophy unit cell and stench are false single
The respective Zymolysis Equipment of born of the same parents bacterium and fermentation process are prior art, but present inventors have unexpectedly found that, by above-mentioned huge gemma
Bacillus, bacillus cereus, the bacterium solution of germ oligotrophy unit cell is combined, and while strengthening respective function, is generated
Similarly hereinafter effect, four kinds of bacterium are mutually promoted, and can more be efficiently completed the reparation to soil, for by heavy metal pollution, have
Machine chemical products contaminated soil can eliminate pollution faster, increase fertility of land, improve remediation efficiency.
In the present invention, the absorption carrier used overcomes the limitation of single carrier, especially, uses mostly in the prior art
Common crop material is as carrier, and the strain life-span prepared by this carrier is shorter, and the survival rate of strain is not high, and the present invention makes
With composite adsorption carrier, activated carbon and diatomite with Different Pore Structures are have selected, strain can be adsorbed on diatomite
Space in, and nutriment can be adsorbed in the hole of activated carbon, coordinated above-mentioned specific nutriment, extended strain
Life-span, improve the present invention strain usage cycles, compared with single use agricultural crop straw, the life of strain
50%-260%, substantially increases economy.
The anthropogenic soil of the present invention efficiently repairs complex micro organism fungicide, is imposed in upper soll layer soil, shallow plowing makes it
Mixed with soil, the nutrition in carrier can be made full use of as carbon source and soil by giving the microorganism in appropriate water source, microbial inoculum
Nitrate in earth has reached good repairing effect as nitrogen source progress vital movement.
Brief description of the drawings
Fig. 1 efficiently repairs the flow chart of the preparation method of complex micro organism fungicide for the anthropogenic soil of the present invention.
Embodiment
Technical scheme is described in further detail with specific embodiment below in conjunction with the accompanying drawings.
Embodiment 1
A kind of anthropogenic soil efficiently repairs the preparation method of complex micro organism fungicide, including:
Step 1: the fermentation of strain
Bacillus megaterium is utilized into Zymolysis Equipment fermenting and producing, the first bacterium solution after being fermented;By waxy gemma bar
Bacterium utilizes Zymolysis Equipment fermenting and producing, the second bacterium solution after being fermented;Germ oligotrophy unit cell is sent out using Zymolysis Equipment
Ferment is produced, the 3rd bacterium solution after being fermented;Pseudomonas putida is utilized into Zymolysis Equipment fermenting and producing, the after being fermented
Four bacterium solutions;Four kinds of bacterium solutions of gained are mixed according to mass ratio 1: 0.1: 0.2: 0.6, mixed bacteria liquid is obtained;
Step 2: the preparation of composite adsorption carrier
By agricultural crop straw using being added to after crusher in crushing in mixer, activated carbon and diatomite are then added, then
Water is added, stirs and granulates, after drying, using high-temp steam sterilizing, vapor (steam) temperature is 126 DEG C, and steam pressure is
0.145MPa, time of contact is standby after 5min sterilizes, and the particle diameter of the complex carrier of preparation is 2mm;Described activated carbon
Specific surface area be 600m2/g;Described diatomaceous specific surface area is 55m2/ g, porosity is 91%;Described crops straw
Stalk, activated carbon, diatomaceous mass ratio are 100: 1: 6;
Step 3: the preparation of microbial inoculum
The mixed bacteria liquid of step one is mixed with the composite adsorption carrier of step 2, while adding appropriate urea, nitric acid
Potassium, calcium superphosphate, borax and amino-oligosacchride;Wherein the consumption of urea, potassium nitrate, calcium superphosphate, borax and amino-oligosacchride is distinguished
It is 10 times, 10 times, 3 times, 1 times and 3 times of mixed bacteria liquid quality;
It is prepared by step 4, finished product
The mixed microbial inoculum of step 3 is crushed to particle diameter for 0.6mm, finished product is obtained, detection viable count is 2.3
Hundred million/gram.
Embodiment 2
A kind of anthropogenic soil efficiently repairs the preparation method of complex micro organism fungicide, including:
Step 1: the fermentation of strain
Bacillus megaterium is utilized into Zymolysis Equipment fermenting and producing, the first bacterium solution after being fermented;By waxy gemma bar
Bacterium utilizes Zymolysis Equipment fermenting and producing, the second bacterium solution after being fermented;Germ oligotrophy unit cell is sent out using Zymolysis Equipment
Ferment is produced, the 3rd bacterium solution after being fermented;Pseudomonas putida is utilized into Zymolysis Equipment fermenting and producing, the after being fermented
Four bacterium solutions;Four kinds of bacterium solutions of gained are mixed according to mass ratio 3: 0.6: 0.5: 1.3, mixed bacteria liquid is obtained;
Step 2: the preparation of composite adsorption carrier
By agricultural crop straw using being added to after crusher in crushing in mixer, activated carbon and diatomite are then added, then
Water is added, stirs and granulates, after drying, using high-temp steam sterilizing, vapor (steam) temperature is 126 DEG C, and steam pressure is
0.145MPa, time of contact is standby after 5min sterilizes, and the particle diameter of the complex carrier of preparation is 8mm;Described activated carbon
Specific surface area be 1100m2/g;Described diatomaceous specific surface area is 75m2/ g, porosity is 91%;Described crops
Stalk, activated carbon, diatomaceous mass ratio are 200: 5: 13
Step 3: the preparation of microbial inoculum
The mixed bacteria liquid of step one is mixed with the composite adsorption carrier of step 2, while adding appropriate urea, nitric acid
Potassium, calcium superphosphate, borax and amino-oligosacchride;Wherein the consumption of urea, potassium nitrate, calcium superphosphate, borax and amino-oligosacchride is distinguished
It is 20 times, 15 times, 10 times, 5 times and 12 times of mixed bacteria liquid quality;
It is prepared by step 4, finished product
The mixed microbial inoculum of step 3 is crushed to particle diameter for 2mm, finished product is obtained.Detect viable count be 4.3 hundred million/
Gram.
Embodiment 3
A kind of anthropogenic soil efficiently repairs the preparation method of complex micro organism fungicide, including:
Step 1: the fermentation of strain
Bacillus megaterium is utilized into Zymolysis Equipment fermenting and producing, the first bacterium solution after being fermented;By waxy gemma bar
Bacterium utilizes Zymolysis Equipment fermenting and producing, the second bacterium solution after being fermented;Germ oligotrophy unit cell is sent out using Zymolysis Equipment
Ferment is produced, the 3rd bacterium solution after being fermented;Pseudomonas putida is utilized into Zymolysis Equipment fermenting and producing, the after being fermented
Four bacterium solutions;Four kinds of bacterium solutions of gained are mixed according to mass ratio 2: 0.6: 0.4: 0.9, mixed bacteria liquid is obtained;
Step 2: the preparation of composite adsorption carrier
By agricultural crop straw using being added to after crusher in crushing in mixer, activated carbon and diatomite are then added, then
Water is added, stirs and granulates, after drying, using high-temp steam sterilizing, vapor (steam) temperature is 126 DEG C, and steam pressure is
0.145MPa, time of contact is standby after 5min sterilizes, and the particle diameter of the complex carrier of preparation is 3mm;Described activated carbon
Specific surface area be 800m2/g;Described diatomaceous specific surface area is 60m2/ g, porosity is 91%;Described crops straw
Stalk, activated carbon, diatomaceous mass ratio are 160: 4: 9;
Step 3: the preparation of microbial inoculum
The mixed bacteria liquid of step one is mixed with the composite adsorption carrier of step 2, while adding appropriate urea, nitric acid
Potassium, calcium superphosphate, borax and amino-oligosacchride;Wherein the consumption of urea, potassium nitrate, calcium superphosphate, borax and amino-oligosacchride is distinguished
It is 10-20 times, 10-15 times, 3-10 times, 1-5 times and 3-12 times of mixed bacteria liquid quality;
It is prepared by step 4, finished product
The mixed microbial inoculum of step 3 is crushed to particle diameter for 2mm, finished product is obtained, detection viable count is 6.1 hundred million/
Gram.
Above example is used for illustrative purposes only, rather than limitation of the present invention, about the technology people of technical field
Member, without departing from the spirit and scope of the present invention, can also make various conversion or modification, therefore all equivalent
Technical scheme should also belong to scope of the invention, should be limited by each claim.
Claims (7)
1. a kind of anthropogenic soil efficiently repairs complex micro organism fungicide, it is characterised in that after complex micro organism fungicide fermentation
Bacterium solution, and composite adsorption carrier, the mass ratio of bacterium solution and composite adsorption carrier is 0.01-0.2: 1-5, and viable count is 2.2-
8.6 hundred million/gram;
Wherein, described microbial bacterial agent includes bacillus megaterium, bacillus cereus, germ oligotrophy unit cell and evil
Smelly pseudomonad;Wherein, described bacillus megaterium, bacillus cereus, germ oligotrophy unit cell and stench are false single
The bacterium solution mass ratio of born of the same parents bacterium is 1-3: 0.1-0.6: 0.2-0.5: 0.6-1.3;
Described composite adsorption carrier includes agricultural crop straw, activated carbon and diatomite;The particle diameter of the complex carrier is
0.6-10mm;Wherein, described agricultural crop straw, activated carbon, diatomaceous mass ratio are 100-200: 1-5: 6-13.
2. a kind of anthropogenic soil according to claim 1 efficiently repairs complex micro organism fungicide, it is characterised in that described
The bacterium solution mass ratio of bacillus megaterium, bacillus cereus, germ oligotrophy unit cell and pseudomonas putida is 2: 0.6
∶0.4∶0.9。
3. a kind of anthropogenic soil according to claim 1 efficiently repairs complex micro organism fungicide, it is characterised in that described
Agricultural crop straw, activated carbon, diatomaceous mass ratio are 160: 4: 9.
4. a kind of anthropogenic soil according to claim 1 efficiently repairs complex micro organism fungicide, it is characterised in that described
Agricultural crop straw is maize straw, wheat stalk or broomcorn straw.
5. a kind of anthropogenic soil described in claim 1 efficiently repairs the preparation method of complex micro organism fungicide, it is characterised in that
Including:
Step 1: the fermentation of strain
Bacillus megaterium is utilized into Zymolysis Equipment fermenting and producing, the first bacterium solution after being fermented;By bacillus cereus profit
Zymolysis Equipment fermenting and producing is used, the second bacterium solution after being fermented;Germ oligotrophy unit cell is fermented using Zymolysis Equipment and given birth to
Production, the 3rd bacterium solution after being fermented;Pseudomonas putida is utilized into Zymolysis Equipment fermenting and producing, the 4th bacterium after being fermented
Liquid;Four kinds of bacterium solutions of gained are mixed according to mass ratio 1-3: 0.1-0.6: 0.2-0.5: 0.6-1.3, mixed bacteria liquid is obtained;
Step 2: the preparation of composite adsorption carrier
By agricultural crop straw using being added to after crusher in crushing in mixer, activated carbon and diatomite are then added, is added
Water, stirs and granulates, after drying, using high-temp steam sterilizing, and vapor (steam) temperature is 126 DEG C, and steam pressure is 0.145MPa,
Time of contact is 5min, standby after sterilizing, and the particle diameter of the complex carrier of preparation is 0.6-10mm;
Step 3: the preparation of microbial inoculum
The mixed bacteria liquid of step one is mixed with the composite adsorption carrier of step 2, while adding appropriate urea, potassium nitrate, mistake
Calcium phosphate, borax and amino-oligosacchride;Wherein the consumption of urea, potassium nitrate, calcium superphosphate, borax and amino-oligosacchride is mixing respectively
10-20 times, 10-15 times, 3-10 times, 1-5 times and 3-12 times of bacterium solution quality;
Step 4, the preparation of finished product
The mixed microbial inoculum of step 3 is crushed to particle diameter for 0.6-10mm, finished product is obtained.
6. preparation method according to claim 5, it is characterised in that the specific surface area of described activated carbon is 600-
1100m2/g;Described diatomaceous specific surface area is 50-75m2/ g, porosity is up to more than 90%.
7. a kind of anthropogenic soil described in claim 1 efficiently repairs application of the complex micro organism fungicide in soil remediation, its
It is characterised by, described complex micro organism fungicide and conventional fertilizer application is mixed and imposed in upper soll layer soil, repairing efficiency is plant
Growth cycle.
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Cited By (8)
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CN109651016A (en) * | 2018-12-28 | 2019-04-19 | 深圳合民生物科技有限公司 | A kind of microbial organic fertilizer and preparation method thereof |
CN110950709A (en) * | 2019-11-11 | 2020-04-03 | 江西普瑞丰生态科技有限公司 | Conditioner for repairing heavy metal soil and preparation method thereof |
CN112552109A (en) * | 2020-12-08 | 2021-03-26 | 河北邢化生态环境工程有限公司 | Diatom carbon microbial compound microbial agent and preparation and application thereof |
CN113004084A (en) * | 2021-03-16 | 2021-06-22 | 吉林省盛达生物质科技有限公司 | Biological bacterial fertilizer and preparation method thereof |
CN113122530A (en) * | 2021-04-19 | 2021-07-16 | 青岛理工大学 | Diatomite-loaded biological iron-manganese oxide material and preparation method and application thereof |
CN113861997A (en) * | 2021-11-02 | 2021-12-31 | 创想未来生物工程(北京)有限公司 | Heavy metal passivation and soil remediation type microbial agent and preparation method thereof |
CN115532816A (en) * | 2022-09-22 | 2022-12-30 | 江西省红壤及种质资源研究所 | Method for promoting quick degradation of organochlorine and organophosphorus pesticides in soil |
CN116948878A (en) * | 2023-06-15 | 2023-10-27 | 青岛农业大学 | Pseudomonas putida and application thereof in straw decomposition |
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Cited By (11)
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CN109651016A (en) * | 2018-12-28 | 2019-04-19 | 深圳合民生物科技有限公司 | A kind of microbial organic fertilizer and preparation method thereof |
CN110950709A (en) * | 2019-11-11 | 2020-04-03 | 江西普瑞丰生态科技有限公司 | Conditioner for repairing heavy metal soil and preparation method thereof |
CN112552109A (en) * | 2020-12-08 | 2021-03-26 | 河北邢化生态环境工程有限公司 | Diatom carbon microbial compound microbial agent and preparation and application thereof |
CN113004084A (en) * | 2021-03-16 | 2021-06-22 | 吉林省盛达生物质科技有限公司 | Biological bacterial fertilizer and preparation method thereof |
CN113122530A (en) * | 2021-04-19 | 2021-07-16 | 青岛理工大学 | Diatomite-loaded biological iron-manganese oxide material and preparation method and application thereof |
CN113122530B (en) * | 2021-04-19 | 2022-11-04 | 青岛理工大学 | Diatomite-loaded biological iron-manganese oxide material and preparation method and application thereof |
CN113861997A (en) * | 2021-11-02 | 2021-12-31 | 创想未来生物工程(北京)有限公司 | Heavy metal passivation and soil remediation type microbial agent and preparation method thereof |
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CN115532816A (en) * | 2022-09-22 | 2022-12-30 | 江西省红壤及种质资源研究所 | Method for promoting quick degradation of organochlorine and organophosphorus pesticides in soil |
CN115532816B (en) * | 2022-09-22 | 2023-12-12 | 江西省红壤及种质资源研究所 | Method for promoting quick degradation of organic chloride and organic phosphorus pesticide in soil |
CN116948878A (en) * | 2023-06-15 | 2023-10-27 | 青岛农业大学 | Pseudomonas putida and application thereof in straw decomposition |
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Application publication date: 20170915 |