CN100569079C - A kind of wettable powder for fungi spore - Google Patents

A kind of wettable powder for fungi spore Download PDF

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Publication number
CN100569079C
CN100569079C CNB2005100890875A CN200510089087A CN100569079C CN 100569079 C CN100569079 C CN 100569079C CN B2005100890875 A CNB2005100890875 A CN B2005100890875A CN 200510089087 A CN200510089087 A CN 200510089087A CN 100569079 C CN100569079 C CN 100569079C
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China
Prior art keywords
mould
powder
broom
sticking
spore
Prior art date
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Expired - Fee Related
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CNB2005100890875A
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Chinese (zh)
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CN1907036A (en
Inventor
张拥华
李世东
缪作清
郭荣君
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Institute of Environment and Sustainable Development in Agriculturem of CAAS
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Institute of Environment and Sustainable Development in Agriculturem of CAAS
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Application filed by Institute of Environment and Sustainable Development in Agriculturem of CAAS filed Critical Institute of Environment and Sustainable Development in Agriculturem of CAAS
Priority to CNB2005100890875A priority Critical patent/CN100569079C/en
Publication of CN1907036A publication Critical patent/CN1907036A/en
Application granted granted Critical
Publication of CN100569079C publication Critical patent/CN100569079C/en
Expired - Fee Related legal-status Critical Current
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention provides by diatomite 85-90%, lignin 1-5%, carboxymethyl cellulose 0.1-1%, SODLUM FULVATE 0.01-0.2% and wettable powder for fungi spore that sclerotium germ, ash arrhizus bacteria, sheath blight fungus, pine root fungus are made up of the mould fungal spore powder of the sticking broom of hyperparasitism.More than each percentages of ingredients all be weight percentage.Described wetting powder can guarantee the biologically active and the control efficiency of fungal spore in producing, store, using.

Description

A kind of wettable powder for fungi spore
Technical field:
The present invention relates to microbial technology field, specifically a kind of wettable powder for fungi spore.
Background technology:
Compare with chemical pesticide, the formulation processing of living microorganism agricultural chemicals is more difficult.At first, microorganism is water-fast organism, and the hydrophobicity of its particle directly influences the physical properties such as wetability, dispersiveness and suspension of preparation.Secondly, as organism, microorganism environmental factor such as temperature, humidity and illumination etc. to external world is relatively more responsive, the preparation stored poor stability, and speed of action is slow, and the field lasting period is short; So when selecting auxiliary agent, except that need are considered the requirement of preparation physicochemical property, also will consider to select some special auxiliary agent, as preventing light agent, synergist etc.Moreover microorganism is as live body, with the compatibility of various auxiliary agents generally than chemical pesticide difference, some auxiliary agent may can not use fully, must select the auxiliary agent compatible with living microorganism.Therefore, the selection of auxiliary agent is vital to the formulation processing of living microorganism agricultural chemicals.
In the living microorganism agricultural chemicals, fungal spore can be made into pulvis, granule or wetting powder etc., but pulvis generally uses duster, can not convert water spray, requires to use under calm condition, and the shortcoming of drift contaminated environment is arranged; Granule is the product forms of using in the soil, and by manually spreading fertilizer over the fields or mixing soil and use, general consumption is big.Bring into play from active ingredient distributing homogeneity and drug effect, both are not as liquid preparation, and wetting powder is the good selection of fungal spore preparation formulation processing.
Summary of the invention:
At the problems referred to above that prior art exists, the present invention has finally developed a kind of wettable powder for fungi spore that can keep the sticking mould spore activity of broom.Therefore, the purpose of this invention is to provide the mould spore wetting powder of a kind of sticking broom, described pulvis can guarantee the biologically active and the control efficiency of spore in producing, store, using.
One embodiment of the invention provide the mould spore wetting powder of a kind of sticking broom, and described pulvis is made up of diatomite, lignin, carboxymethyl cellulose, SODLUM FULVATE and the mould conidial powder of sticking broom.
One embodiment of the invention provide the mould spore wetting powder of a kind of sticking broom, described pulvis is made up of diatomite 85-90%, lignin 1-5%, carboxymethyl cellulose 0.1-1%, SODLUM FULVATE 0.01-0.2% and the mould conidial powder of sticking broom, and each percentages of ingredients all is weight percentage.
Another embodiment of the present invention provides by diatomite 85-90%, lignin 1-5%, carboxymethyl cellulose 0.1-1%, SODLUM FULVATE 0.01-0.2% and the mould spore wetting powder of sticking broom that sclerotium germ, ash arrhizus bacteria, sheath blight fungus, pine root fungus are made up of the mould conidial powder of sticking broom of hyperparasitism.
Another embodiment of the present invention provides the mould spore wetting powder of being made up of diatomite 85-90%, lignin 1-5%, carboxymethyl cellulose 0.1-1%, SODLUM FULVATE 0.01-0.2% and the mould HLD-1 bacterial strain of sticking broom conidial powder of sticking broom; Biomaterial glues the mould HLD-1 strain classification of broom called after Gliocladium sp., China Committee for Culture Collection of Microorganisms common micro-organisms center (deposit number is CGMCC No.1037), this depositary institution address: No. 13, North No.1 Row, Zhongguancun, Haidian District, Beijing City have been preserved in on November 21st, 2003.
The used mould conidial powder of sticking broom can be the mould fungal spore powder of any sticking broom that above-mentioned plant pathogenic fungi is had hyperparasitism among the present invention.
Used diatomite is carrier in the sticking mould spore wetting powder of broom of the present invention, requires purity greater than 95%; Lignin is also referred to as M-11 or lignosulfonates, and as wetting dispersing agent, purity requirement is greater than 94%; Carboxymethyl cellulose requires purity greater than 95% as stabilizing agent; SODLUM FULVATE is as uv-protector, and requirement is a black powder, and is soluble in water, and purity is greater than 95%.
The mould conidial powder of sticking broom used in the wetting powder of the present invention can be pure conidial powder, and purity is greater than 90%, and water content is less than 10%.
The preparation method of wetting powder of the present invention adds mixer with each component successively, for example in separation of CC-II type fungal spore and the mixer, stirs while adding, and mixes fully until each component.
The using method of wetting powder of the present invention is that described pulvis is converted water spray, and the pure conidial powder of 5 grams is used on general every mu of ground.
Embodiment
Embodiment 1: the preparation of the sticking mould spore wetting powder of broom
Component Content (weight %)
Diatomite 87.3%
Lignin 2%
Carboxymethyl cellulose 0.5%
SODLUM FULVATE 0.2%
Sticking broom mould (Gliocladium sp.) fungal spore powder 10%
Diatomite, lignin, carboxymethyl cellulose, SODLUM FULVATE and the mould fungal spore powder of pure sticking broom added successively that CC-II type fungal spore separates and mixer in, stir while adding, mix fully until each component.
Embodiment 2: the preparation of the sticking mould spore wetting powder of broom
Component Content (weight %)
Diatomite 80.8%
Lignin 3%
Carboxymethyl cellulose 1%
SODLUM FULVATE 0.2%
Pink sticking broom mould (Gliocladium roseum) fungal spore powder 15%
Diatomite, lignin, carboxymethyl cellulose, SODLUM FULVATE and the mould fungal spore powder of pink sticking broom added successively that CC-II type fungal spore separates and mixer in, stir while adding, mix fully until each component.
Embodiment 3: the preparation of the mould spore wetting powder of sticking broom of control wheat sharp eyespot
Component Content (weight %)
Diatomite 85.65%
Lignin 4%
Carboxymethyl cellulose 0.3%
SODLUM FULVATE 0.05%
The sticking mould HLD-1 bacterial strain of broom fungal spore powder 10%
Diatomite, lignin, carboxymethyl cellulose, SODLUM FULVATE and the mould HLD-1 bacterial strain of sticking broom fungal spore powder added successively that CC-II type fungal spore separates and mixer in, stir while adding, mix fully until each component.

Claims (5)

1, the mould spore wetting powder of a kind of sticking broom, it is characterized in that, described pulvis is made up of diatomite 85-90%, lignin 1-5%, carboxymethyl cellulose 0.1-1%, SODLUM FULVATE 0.01-0.2% and the mould conidial powder of sticking broom, and each percentages of ingredients all is weight percentage.
2, the mould spore wetting powder of sticking broom as claimed in claim 1 is characterized in that, the mould conidial powder of described sticking broom is the sticking mould pure conidial powder of broom, and purity is greater than 90%, and water content is less than 10%.
3, the mould spore wetting powder of sticking broom as claimed in claim 1 or 2 is characterized in that used diatomite is carrier, requires purity greater than 95%; Lignin is also referred to as M-11 or lignosulfonates, and as wetting dispersing agent, purity requirement is greater than 94%; Carboxymethyl cellulose requires purity greater than 95% as stabilizing agent; SODLUM FULVATE is as uv-protector, and requirement is a black powder, and is soluble in water, and purity is greater than 95%.
4, the mould spore wetting powder of sticking broom as claimed in claim 1 is characterized in that, the mould conidial powder of described sticking broom is the mould conidial powder of sticking broom that sclerotium germ, ash arrhizus bacteria, sheath blight fungus, pine root fungus is had hyperparasitism.
5, the mould spore wetting powder of sticking broom as claimed in claim 1 is characterized in that, the mould conidial powder of described sticking broom is the sticking mould HLD-1 conidial powder of broom, and preserving number is CGMCC No.1037.
CNB2005100890875A 2005-08-05 2005-08-05 A kind of wettable powder for fungi spore Expired - Fee Related CN100569079C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CNB2005100890875A CN100569079C (en) 2005-08-05 2005-08-05 A kind of wettable powder for fungi spore

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Application Number Priority Date Filing Date Title
CNB2005100890875A CN100569079C (en) 2005-08-05 2005-08-05 A kind of wettable powder for fungi spore

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CN1907036A CN1907036A (en) 2007-02-07
CN100569079C true CN100569079C (en) 2009-12-16

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101451107B (en) * 2007-12-07 2011-11-23 中国农业科学院植物保护研究所 Method for large scale preparing Gliocladium chlamydospore

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102174460B (en) * 2011-01-23 2012-07-18 青岛科技大学 Gliocladium roseum chlamydospore and method for producing wettable powder thereof
CN102391952B (en) * 2011-11-11 2013-07-17 中国农业科学院烟草研究所 In vitro conservation method of puccinia cnici
CN103484421B (en) * 2013-04-03 2016-03-23 中国农业科学院植物保护研究所 A kind of chlamydosporic method of liquid fermenting scale up test Gliocladium roseum
CN104304328A (en) * 2014-09-27 2015-01-28 江苏天象生物科技有限公司 A preparing method of a hyperparasitic fungal inoculant for sclerotinia sclerotiorum
CN104663731B (en) * 2015-02-09 2017-11-10 河北农业大学 A kind of bactericidal composition and its application
CN106614759B (en) * 2016-12-23 2019-05-03 江西天祥通用航空股份有限公司 It is a kind of at green meat seat bacterium wettable powder and preparation method and application
CN115413677A (en) * 2022-10-21 2022-12-02 北京启高生物科技有限公司 Gliocladium roseum preparation for spraying of unmanned aerial vehicle and application method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000018241A1 (en) * 1998-09-30 2000-04-06 Her Majesty The Queen In Right Of Agriculture Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada Gliocladium roseum strains useful for the control of fungal pathogens in plants
CN1156223C (en) * 2002-12-16 2004-07-07 张泽华 Wettability powder of fungi spore

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000018241A1 (en) * 1998-09-30 2000-04-06 Her Majesty The Queen In Right Of Agriculture Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada Gliocladium roseum strains useful for the control of fungal pathogens in plants
CN1156223C (en) * 2002-12-16 2004-07-07 张泽华 Wettability powder of fungi spore

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101451107B (en) * 2007-12-07 2011-11-23 中国农业科学院植物保护研究所 Method for large scale preparing Gliocladium chlamydospore

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