CN106614759B - It is a kind of at green meat seat bacterium wettable powder and preparation method and application - Google Patents

It is a kind of at green meat seat bacterium wettable powder and preparation method and application Download PDF

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Publication number
CN106614759B
CN106614759B CN201611207591.5A CN201611207591A CN106614759B CN 106614759 B CN106614759 B CN 106614759B CN 201611207591 A CN201611207591 A CN 201611207591A CN 106614759 B CN106614759 B CN 106614759B
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green meat
meat seat
seat bacterium
wettable powder
parts
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CN106614759A (en
Inventor
石仕福
李肖宇
石峥
梁小文
王根豪
曾升华
严艺波
李英武
罗建辉
罗双燕
吴俊杰
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Jiangxi Tianxiang General Aviation Co Ltd
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Jiangxi Tianxiang General Aviation Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/12Powders or granules
    • A01N25/14Powders or granules wettable

Abstract

The present invention provides a kind of into green meat seat bacterium wettable powder, the component including following parts by weight: 2~10 parts of medicine activity component, the medicine activity component is into green meat seat bacterium;5.4~9.5 parts of auxiliary agent;78.5~94.6 parts of filler.In the present invention, efficient at green meat seat bacterium wettable powder, low toxicity, insecticidal effect are stablized, and to people and animals and crop safety, highly-safe.The experimental results showed that reaching as high as 80% or more at the effect of green meat seat bacterium wettable powder pest control.

Description

It is a kind of at green meat seat bacterium wettable powder and preparation method and application
Technical field
The present invention relates to technical field of pesticide, and in particular to arrives a kind of at green meat seat bacterium wettable powder and preparation method thereof With the application in prevention and treatment crop pests.
Background technique
China is large agricultural country, and for thousands of years always based on planting industry, agricultural is the source of the basic necessities of life of the mankind, survives it This, is that other departments are able to independent and development basis, is the basis of national economy, therefore the development of agricultural will directly affect The development of national economy.But in agricultural production, the generation of insect pest often seriously affects crop growth, make crops from A series of variation and destruction has occurred to institutional framework in physiological function, and then leads to a large amount of underproduction of crops, to grower with Huge economic loss is carried out.Therefore to crop pests effectively prevent be it is very urgent, to guarantee grain security and kind The economic interests of plant person.
At present there are many kinds of the types of crop pests, wherein nematode can be invaded and parasitic plant, by invasion and parasitic Plant influences normal growth and development because nematode absorbs endotrophic, and the secretion of nematode metabolism can stimulate host plant Cell and tissue, cause plant lopsided.Most plants nematode encroaches on the under ground portion of plant, such as root, stem tuber, root can It shows as that dross, necrosis, root is short and thick and grows thickly, leads to a large amount of underproduction of crops or quality degradation.Common Plant nematode Disease has root knot nematode disease, soybean cyst nematode, wheat anguina disease, sweep stem nematode, aphelenchoides besseyi disease, citrus half Puncture nematodiasis etc..
In the prior art, usually disease-resistant variety or chemical insecticide is selected to carry out pest control disease.Using chemical insecticide When being prevented and treated, although control efficiency is ideal, chemical insecticide has difficult to degrade, be easy to cause soil hardening, destroys life The balance of state system, while people and animals are endangered larger.
Summary of the invention
In view of this, it is an object of the invention to not allow problem of easy degradation for insecticide, and provide a kind of at green meat Seat bacterium wettable powder and preparation method thereof, while making the wettable powder that there is safe and non-toxic, preferable insecticidal effect It keeps soil from packing together, is conducive to improve crop yield.
The present invention provides a kind of into green meat seat bacterium wettable powder, the component including following parts by weight:
5~8 parts of active component, the active component is into green meat seat bacterium;
5.8~8.2 parts of auxiliary agent;
89.2~73.8 parts of filler.
Preferably, the component including following parts by weight:
6 parts of active component, the active component is into green meat seat bacterium;
6 parts of auxiliary agent;
88 parts of filler.
Preferably, effective spore amount at green meat seat bacterium wettable powder is 200~40,000,000,000 CFU/ grams.
Preferably, effective spore amount at green meat seat bacterium wettable powder is 30,000,000,000 CFU/ grams.
Preferably, work spore rate >=90% of the green meat seat bacterium at green meat seat bacterium wettable powder.
Preferably, the auxiliary agent includes one of iso-octyl sodium succinate, Tween-60, D-425, EFW and the wooden sodium or several Kind.
Preferably, the filler includes one or more of white carbon black, calcined diatomite and calcined kaolin.
The present invention provides the preparation methods at green meat seat bacterium wettable powder, comprising the following steps:
1) filler, active component and auxiliary agent are mixed, obtains mixture;
2) mixture that the step 1) obtains is crushed, obtains into green meat seat bacterium wettable powder.
The present invention provide it is described at green meat seat bacterium wettable powder or the method be prepared at green meat seat bacterium Application of the wettable powder in prevention and treatment crop pests.
Preferably, the crop pests are tomato root-knot eelworm, tomato trialeurodes vaporariorum, aphid and underground root-knot nematode.
The present invention provides a kind of into green meat seat bacterium wettable powder, the component including following parts by weight: active component 5 ~8 parts, the active component is into green meat seat bacterium;5.8~8.2 parts of auxiliary agent;89.2~73.8 parts of filler.In the present invention, at The active material that green meat seat bacterium wettable powder plays insecticidal action is to be reached at green meat seat bacterium by secreting metabolin at green meat seat bacterium To insecticidal action.And it is adapted in the soil fastly at green meat seat bacterium, steadily in the long term, therefore there is efficient, low toxicity, insecticidal effect is steady It is fixed, and to people and animals and crop safety, it is highly-safe.In addition at green meat seat bacterium as microorganism, growth metabolism process is conducive to soil Granule is formed, and soil hardening is effectively prevent.The experimental results showed that at the effect highest of green meat seat bacterium wettable powder pest control Up to 80% or more.
Biological deposits explanation
At green meat seat bacterium (Hypocrea virens), it is commonly micro- to be preserved in China Committee for Culture Collection of Microorganisms Bio-Centers (CGMCC), preservation address are as follows: the Chinese Beijing Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the Chinese Academy of Sciences Institute of Micro-biology, the deposit date is on 08 13rd, 2013, deposit number was CGMCC No.7995.
Specific embodiment
The present invention provides a kind of into green meat seat bacterium wettable powder, the component including following parts by weight:
5~8 parts of active component, the active component is into green meat seat bacterium;
5.8~8.2 parts of auxiliary agent;
89.2~73.8 parts of filler.
The active component provided by the invention that 5~8 parts by weight are preferably included at green meat seat bacterium wettable powder, preferably 6 Part.
In the present invention, described preferably to be lived at green meat seat bacterium with the strain that biological deposits number is CGMCC No.7994 Change culture to obtain.
The activation culture method at green meat seat bacterium, including the following steps: 1) will be inoculated at green meat seat bacterium actication of culture It is cultivated into first order seed liquid culture medium, obtains primary seed solution;2) primary seed solution for obtaining the step 1) is inoculated with Fermented and cultured is carried out to secondary liquid culture medium, obtains secondary seed solution;3) secondary seed solution for obtaining the step 2) with Solid medium mixed culture, obtains into green meat seat bacterium spore;The solid medium includes following mass percentage component, Wheat bran 65.00%~75.00%, dregs of beans 7.00%~12.00%, corn flour 7.60%~13.50%, husk 7.80%~ 13.40%.
The present invention provides a kind of fermentation method for producing at green meat seat bacterium, will be at green meat seat bacterium actication of culture, in level-one Seed fluid nutrient mediums of saccharomycete and secondary liquid culture medium, which are cultivated, obtains strong thallus and a certain amount of spore with expanding propagation Secondary seed solution is inoculated on the solid medium that compound carbon source and nitrogen source are formed, makes into the fast fast-growing of green meat seat bacterium by son Long and production spore.
The present invention will at green meat seat bacterium actication of culture, activated at green meat seat bacterium strain.The present invention is to the activation Method be not particularly limited, using the technical solution of activation well known to those skilled in the art.In implementation of the invention It is described to be provided at green meat seat bacterium strain for Jiangxi Tianren Ecology Co., Ltd. in example.
In the present invention, the culture medium when activation is preferably potato dextrose agar.
Activated at green meat seat bacterium, the present invention is inoculated into the training of first order seed liquid at green meat seat bacterium for the activation It supports and is cultivated in base, obtain primary seed solution.
In the present invention, the time of the culture is preferably 35~50h, more preferably 40~45h.The temperature of the culture Preferably 25~32 DEG C, more preferably 30 DEG C of degree.The culture preferably carries out under illumination condition, and the intensity of illumination cultivation is 2000~3000lx.
In the present invention, the culture preferably carries out under stirring conditions, and the frequency of the stirring is 1~2 time/ 20min, the time stirred every time are 1~2min, and the frequency of the stirring is 150~300r/min.
In the present invention, the incubation is ventilated, and the program of the ventilation is particularly preferred as: entering culture In for 24 hours, ventilatory capacity is preferably 10~16m3/ h, more preferably 15m3/h;Into after culture for 24 hours, ventilatory capacity is preferably 15~ 24m3/ h, more preferably 20m3/h。
In the present invention, the inoculum concentration of the culture is preferably 1~5%.
In the present invention, the initial pH value of the level liquid culture medium is preferably 5.5~6.5, and more preferably 6.0.
In the present invention, the first order seed liquid culture medium preferably includes the component of following mass percentage: grape Sugar 0.50%~2.00%, white granulated sugar 0.50%~2.00%, soybean powder 0.10%~0.30%, ammonium sulfate 0.10%~ 0.30%, dipotassium hydrogen phosphate 0.05%~0.10%, potassium chloride 0.05%~0.10%, magnesium sulfate 0.01%~0.04%, sulphur Sour iron 0.00%~0.004%, surplus are water;It more preferably include following mass percentage component, glucose 1%, white granulated sugar 1%, soybean powder 1%, ammonium sulfate 0.2%, dipotassium hydrogen phosphate 0.2%, potassium chloride 0.08%, magnesium sulfate 0.02%, ferric sulfate 0.0002%, surplus is water.The present invention is not particularly limited the source of above-mentioned drug, and use is well known to those skilled in the art The drug for being used to prepare culture medium.
After obtaining primary seed solution, the primary seed solution is inoculated into secondary liquid culture medium by the present invention ferments Culture, obtains secondary seed solution.
The present invention is not particularly limited the method for the inoculation, using well known inoculation described in those skilled in the art Technical solution.
In the present invention, the inoculum concentration of the fermented and cultured is preferably 8~15%, and more preferably 10%.
In the present invention, the time of the fermented and cultured is preferably 55~70h, more preferably 60~64h.
In the present invention, the fermented and cultured temperature is 28~32 DEG C, more preferably 30 DEG C.
In the present invention, the fermented and cultured need to carry out under illumination condition, and the intensity of the illumination cultivation is 3000 ~4000lx.
In the present invention, the fermented and cultured preferably carries out under aeration condition, and the program of the ventilation is specifically preferred Are as follows: after being inoculated into secondary liquid culture medium for 24 hours within, ventilatory capacity be 100~180m3/ h, more preferably 120~160m3/ h, Most preferably 150m3/h;After culture for 24 hours, ventilatory capacity is 200~260m3/ h, more preferably 220~250m3/ h, most preferably 240m3/h。
In the present invention, the culture in the step 2) carries out under stirring conditions, and the frequency of the stirring is 1~2 Secondary/20min, the time stirred every time are 1~2min, and stirring frequency is 150~300r/min.
In the present invention, the pH value of fermentation liquid is without regulating and controlling during the fermented and cultured, natural ph.
In the present invention, the secondary seed liquid culture medium preferably includes following mass percentage component: grape Sugared 1%~3%, white granulated sugar 1%~3%, dehydrated potato powder 0.5%~1.5%, soybean powder 0.5%~1.5%, corn flour 0.3% ~1.0%, dipotassium hydrogen phosphate 0.01%~0.02%, surplus are water;It more preferably include following mass percentage component: grape Sugared 2%, white granulated sugar 2%, dehydrated potato powder 1%, soybean powder 1%, corn flour 0.5%, dipotassium hydrogen phosphate 0.01%, surplus is water.
In the present invention, effective spore amount at green meat seat bacterium wettable powder is preferably 200~40,000,000,000 CFU/ grams, More preferably 30,000,000,000 CFU/ grams.Work spore rate >=90% at green meat seat bacterium at green meat seat bacterium wettable powder, more preferably It is >=95%.
It is provided by the invention to include 5.8 at green meat seat bacterium wettable powder on the basis of the active component of 5~8 parts by weight ~8.2 parts of auxiliary agents, preferably 6 parts.The present invention is not particularly limited the type of the auxiliary agent and source, using art technology The commercial goods of the acceptable auxiliary agent of agricultural wettable pulvis known to personnel.In the present invention, the auxiliary agent is preferably One or more of iso-octyl sodium succinate, Tween-60, D-425, EFW and the wooden sodium, when the auxiliary agent is that various ingredients combine When, specially two kinds, three kinds, four kinds or five kinds.In the present invention, it when the auxiliary agent is four kinds of group subassemblys, preferably spits Temperature -60, D-425, EFW and the wooden sodium;The weight ratio of the Tween-60, D-425, EFW and the wooden sodium is preferably (3.0~5.0): (2.0~3.0): (0.4~1.5): (1~: 4), more preferably (3.4~4.5): (1.5~2.5): (0.8~1.2): (1.5~ 3.5), most preferably 4:2:1:2.In the present invention, when the auxiliary agent is three kinds of group subassemblys, preferably Tween-60, D- 425 and EFW;The Tween-60, D-425, EFW weight ratio be preferably (3.0~5.0): (2.0~3.0): (0.4~1.5), More preferably (3.4~4.5): (1.5~2.5): (0.8~1.2), most preferably 4:2:1.
In the present invention, the wetting agent EFW is preferably alkylnaphthalene sulfonate and anion humectant mixture, the present invention The source of the wetting agent EFW is not particularly limited, using commercial goods well known to those skilled in the art.In this hair In bright, the auxiliary agent stablizes drug character, improves drug effect.
It is provided by the invention to include 73.8~89.2 at green meat seat bacterium wettable powder on the basis of 5~8 parts of active components The filler of part, preferably 88 parts.In the present invention, the filler is preferably in white carbon black, calcined diatomite and calcined kaolin One or more.The present invention does not have special restriction to the source of the filler, using commercial goods.In the present invention, The filler, can arbitrary proportion mixing specifically preferably to be any several in above-mentioned substance.In the present invention, it is filled out when described When material is two kinds of group subassemblys, it can be white carbon black and calcined diatomite, be particularly preferred as 2.0~4.0 parts of white carbon blacks and 76.5 ~90.6 parts of calcined diatomites, more preferably 2.5~3.5 parts of white carbon blacks and 80.3~88.8 parts of calcined diatomites, most preferably 3 Part white carbon black and 85 parts of calcined diatomites;
It can also be white carbon black and calcined kaolin when the filler is two kinds of group subassemblys, it is particularly preferred as 2.0~ 4.0 parts of white carbon blacks and 76.5~90.6 parts of calcined kaolins, more preferably 2.5~3.5 parts of white carbon blacks and 80.3~88.8 parts are forged Burn kaolin, most preferably 3 parts of white carbon blacks and 85 parts of kaolin.
Filler of the present invention has good medicine stability, and the drug effect of drug also can be improved.
The present invention provides described in above-mentioned technical proposal at the preparation method of green meat seat bacterium wettable powder, including following step It is rapid:
1) filler, active component and auxiliary agent are mixed, obtains mixture;
2) mixture that the step 1) obtains is crushed, obtains into green meat seat bacterium wettable powder.
In the present invention, the mixing of the filler, active component and auxiliary agent is preferably by filler, active component and auxiliary agent point It is not divided into 3~5 parts, more preferably 4 parts, after the 1st part of filler is mixed with the 1st part of active component, then is successively helped with the 1st part Agent, the 2nd part of filler, the 2nd part of active component and the mixing of the 2nd part of auxiliary agent, and so on, until having added all raw materials, then it is excellent Choosing continuess to mix 20~40min, more preferably 25~35min, most preferably 30min.The present invention does not have the mixed equipment There is special limitation, is filled using mixing apparatus well known to those skilled in the art, such as mixing.
In the present invention, the granularity of the filler is preferably 150~300 mesh, more preferably 200 mesh.The present invention will be described Filler preferably carries out grinding and sieving.The present invention does not have special restriction to the method for the crushing, using those skilled in the art The technical solution crushed known to member.In the present invention, described crush specific is preferably air-flowing type crushing, air-flowing type The sealing pressure of crushing is preferably 0.1~0.15MPa, more preferably 0.1~0.15MPa, most preferably 0.13MPa;Air-flow The discharging sealing gland pressure that formula crushes is preferably 0.1~0.2MPa, more preferably 0.15MPa;The pulse that air-flowing type crushes is excellent It is selected as 0.4~0.6MPa, more preferably 0.5MPa;The feed pressure of air-flowing type is preferably 0.75~0.85MPa, more preferably 0.8MPa.The present invention does not have special restriction to the equipment of the crushing, using disintegrating apparatus well known to those skilled in the art ?.The present invention is not particularly limited the method for sieving, using screen method well-known to those skilled in the art.
The aspect provided by the invention that can be applied to crops pest control at green meat seat bacterium wettable powder, provides one The kind application at green meat seat bacterium wettable powder in terms of preventing and treating crop pests.Specifically, applicable farming species Class includes cereal crops, industrial crops, vegetable crop, orchard crop, forage crop etc..It is of the present invention wettable at green meat seat bacterium Property pulvis can prevent and treat tomato root-knot eelworm, tomato trialeurodes vaporariorum, aphid and underground root-knot nematode.
The present invention is not particularly limited the application method at green meat seat bacterium wettable powder, using this field skill Application method known to art personnel.In the present invention, the formulation rate at green meat seat bacterium wettable powder is preferably 25~ 50g/ mus, more preferably 30g/ mus.The application frequency at green meat seat bacterium wettable powder is preferably 5~7 days/time.
Below in conjunction with the embodiment in the present invention, the technical solution in the present invention is clearly and completely described.It is aobvious So, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on the reality in the present invention Example is applied, every other embodiment obtained by those of ordinary skill in the art without making creative efforts all belongs to In the scope of protection of the invention.
Embodiment 1
At the preparation of green meat seat bacterium wettable powder: based on parts by weight, by 3 parts of white carbon blacks, 85 parts of calcined kaolins, 5 Part is divided into 4 at green meat seat bacterium, 4 parts of iso-octyl sodium succinates, 2 parts of naphthalene sulphonates and 1 part of wetting agent EFW respectively Heap after mixing the 1st heap white carbon black with the 1st heap calcined kaolin, then piles green meat seat bacterium with the 1st and mixes, then different pungent with the 1st heap The mixing of base sodium succinate, then mixed with the 1st heap naphthalene sulphonate, then mixed with the 1st heap wetting agent EFW, then white with the 2nd heap Carbon black mixing, and so on, it has been mixed until all raw materials are whole, has been further continued for mixing 30min, obtains mixture;Mixture warp Air-flowing type crushes, and obtains 25 μm of powder to get at green meat seat bacterium wettable powder.
The quality control index at green meat seat bacterium wettable powder of 1 embodiment 2 of table preparation
Embodiment 2
At the preparation of green meat seat bacterium wettable powder: based on parts by weight, by 3 parts of white carbon blacks, 85 parts of calcined diatomites, 5 Part is at green meat seat bacterium, 4 parts of fatty alcohols and ethylene oxide condensate, 2 parts of sodium metnylene bis-naphthalene sulfonates, 1 part of lauryl sodium sulfate It is divided into 4 heaps respectively, after the 1st heap white carbon black is mixed with the 1st heap calcined diatomite, then piles green meat seat bacterium with the 1st and mixes, Mix with the 1st heap fatty alcohol with ethylene oxide condensate, then mixed with the 1st heap sodium metnylene bis-naphthalene sulfonate again, then with the 1st heap ten Sodium dialkyl sulfate mixing, then mixed with the 2nd heap white carbon black, and so on, it has been mixed until all raw materials are whole, has been further continued for mixing 20min is closed, mixture is obtained;Mixture is crushed through air-flowing type, obtains 20 μm of powder to get green meat seat bacterium wettable powder is arrived Agent.
Embodiment 3
The preparation of green meat seat bacterium wettable powder: based on parts by weight, by 3.5 parts of white carbon blacks, 80.3 parts of calcined kaolins, 8 parts average respectively at green meat seat bacterium, 4.5 parts of iso-octyl sodium succinates, 2.5 parts of naphthalene sulphonates and 1.2 parts of wetting agent EFW It is divided into 4 heaps, after the 1st heap white carbon black is mixed with the 1st heap calcined kaolin, then piles green meat seat bacterium with the 1st and mix, then with the 1 The mixing of heap iso-octyl sodium succinate, then mixed with the 1st heap naphthalene sulphonate, then mixed with the 1st heap wetting agent EFW, then with The mixing of 2nd heap white carbon black, and so on, it has been mixed until all raw materials are whole, has been further continued for mixing 30min, obtains mixture;It is mixed It closes material to crush through air-flowing type, obtains 20 μm of powder to get green meat seat bacterium wettable powder is arrived.
Embodiment 4
Pharmacodynamic test: this is tested carries out in Shandong Province, and prevention and treatment kind is tomato, and management is good, in root-knot nematode generation Deng weighting.Experimental field is divided into three regions by this test, and region 1 is control group, only sprays clear water;Region 2 sprays implementation Prepared in example 2 at green 2000 times of dilutions of meat seat bacterium wettable powder;Region 3 sprays 2000 times of 50% phoxim emulsifiable concentrate dilutions The concentration of liquid, active component is identical as region 2;Trizonal dosage is identical, and 1 day after application, 3 days, each investigation in 7 days was primary Borer population living, calculates Revision insect recluced rate.Concrete outcome is as shown in table 2, by the data of table 2 it can be concluded that, the Revision insect recluced rate in region 2 Highest shows that the insecticidal effect prepared by the present invention at green meat seat bacterium wettable powder is best.
Table 2 at green meat seat bacterium wettable powder desinsection (root-knot nematode) effect
From the test data of table 2 it can be concluded that, it is prepared by the present invention at 7 days insect populations of green meat seat bacterium wettable powder decline Rate may be up to 80%, and insecticidal effect is higher than 50% phoxim emulsifiable concentrate.
As seen from the above embodiment, prepared by the present invention at green meat seat bacterium wettable powder, to the control rate of root-knot nematode Up to 80 or more, insecticidal effect is higher than effective cypermethrin.
Embodiment 5
Green meat seat bacterium wettable powder prepared by embodiment 2 is administered to tomato Tanaka, after application 1 month, to tomato field The microbial population number of soil, the content of organic matter and crop yield are counted, and wherein Experimental Area 1 is with being administered to blank Negative control is done, test area 2 is the average value of Examples 1 to 3, and test area 3 applies 50% phoxim emulsifiable concentrate as positive Control group.Statistical result is as shown in table 3 after three Experimental Area application fertilizer.
The green meat seat bacterium wettable powder of table 3 and control group application effect list
The tomato Tanaka's microbial population applied it can be seen from the experimental result of table 3 through green meat seat bacterium wettable powder Quantity is substantially better than other two control groups, and the content of organic matter also has conspicuousness raising, while crop yield reaches It 920kg/ mus, has a clear superiority compared with other two control groups.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered It is considered as protection scope of the present invention.

Claims (9)

1. at green meat seat bacterium wettable powder in the application for preventing and treating crop pests;The crop pests are root-knot nematode;
It is described at green meat seat bacterium wettable powder, the component including following parts by weight:
5~8 parts of active component, the active component is into green meat seat bacterium;
5.8~8.2 parts of auxiliary agent;
73.8~89.2 parts of filler;
The deposit number at green meat seat bacterium is CGMCC No.7995.
2. application according to claim 1, which is characterized in that described at green meat seat bacterium wettable powder includes following weight The component of number:
6 parts of active component, the active component is into green meat seat bacterium;
6 parts of auxiliary agent;
88 parts of filler.
3. application according to claim 1 or claim 2, which is characterized in that effective spore at green meat seat bacterium wettable powder Amount is 200~40,000,000,000 CFU/ grams.
4. applying according to claim 3, which is characterized in that effective spore amount at green meat seat bacterium wettable powder is 30000000000 CFU/ grams.
5. applying according to claim 4, which is characterized in that it is described at green meat seat bacterium wettable powder at green meat seat bacterium Spore rate >=90% living.
6. applying according to claim 1, which is characterized in that the auxiliary agent includes iso-octyl sodium succinate, Tween-60, D- 425, one or more of EFW and the wooden sodium.
7. application according to claim 1 or 6, which is characterized in that the filler includes white carbon black, calcined diatomite and calcining One or more of kaolin.
8. being applied described in claim 1, which is characterized in that the preparation method at green meat seat bacterium wettable powder, including with Lower step:
1) filler, active component and auxiliary agent are mixed, obtains mixture;
2) mixture that the step 1) obtains is crushed, obtains into green meat seat bacterium wettable powder.
9. application according to claim 1, which is characterized in that the root-knot nematode is tomato root-knot eelworm.
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