CN118575813A - A highly effective attractant for broad-legged moth and its application - Google Patents

Abstract

The invention discloses an efficient attractant for broad-legged armyworm and its application. The efficient attractant composition for broad-legged armyworm includes sex pheromone for broad-legged armyworm and a synergist, wherein the sex pheromone component is cis-11-hexadecene-1-ol, and the synergist is one of cis-11-hexadecene-2-bromopropionate or cis-11-hexadecene-2-butenoate. The efficient attractant of the present invention can greatly increase the amount of male insects trapped by broad-legged armyworm, effectively interfere with mating between male and female insects, and reduce the probability of harm to offspring. The raw materials of the attractant composition of the present invention are easy to obtain, easy to use, and environmentally friendly, and can be accurately applied to insect monitoring, large-scale trapping, and mating interference, which is of great significance for green prevention and control of broad-legged armyworm.

Description

A highly effective attractant for broad-legged moth and its application

Technical Field

The invention belongs to the technical field of insect sex pheromone preparation, and particularly relates to a high-efficiency attractant composition for controlling broad-shanked armyworm.

Background Art

Protoschinia Scutosa , also known as the artemisia moth, belongs to the family Noctuidae in the order Lepidoptera and is an important agricultural pest. The larvae feed on the branches, leaves and buds of plants, and in severe cases can eat all the leaves of the plants. The adults are nocturnal and have strong phototaxis and the habit of laying eggs in densely growing plants. They have strong reproductive capacity and a single head can lay thousands of eggs. At present, there are no reports on efficient attractants for Protoschinia Scutosa at home and abroad. Insect pheromones stand out among a number of biological pesticides because of their advantages of trace efficiency, species specificity and environmental non-toxicity, providing a new strategy for green and efficient pest control.

Summary of the invention

The invention aims to provide a high-efficiency attractant composition for Spodoptera exigua and its application.

Based on the above purpose, the present invention provides a high-efficiency attractant composition for Spodoptera exigua, comprising a sex pheromone for Spodoptera exigua and a synergist, wherein the sex pheromone is cis-11-hexadecene-1-ol, and the synergist is one of cis-11-hexadecene-2-bromopropionate or cis-11-hexadecene-2-butenoate.

Optionally, the mass ratio of the sex pheromone of Spodoptera exigua to the synergist is 100:(1~10); the mass of the sex pheromone is 1000 μg, and the mass of the synergist is 1-1000 μg, and the synergist is composed of the following mass components: 1-100 μg of cis-11-hexadecene-2-bromopropionate or 100-1000 μg of cis-11-hexadecene-2-butenoate.

The sex pheromone of Spodoptera exigua is cis-11-hexadecene-1-ol with a mass of 1000 μg.

Preferably, the mass of the Spodoptera exigua sex pheromone is 1000 μg, the mass of the synergist is 10-100 μg, and it is composed of the following components: 10 μg of cis-11-hexadecene-2-bromopropionate or 100 μg of cis-11-hexadecene-2-butenoate.

The invention provides a high-efficiency attractant lure core for preventing and controlling broad-shanked armyworm, which contains the broad-shanked armyworm attractant composition.

The method for preparing a highly effective attractant core for controlling Spodoptera exigua is as follows: first, add a chlorinated paraffin solution containing the sex pheromone of Spodoptera exigua into a red polyethylene capillary, then add 10 μg of cis-11-hexadecene-2-bromopropionate or 100 μg of cis-11-hexadecene-2-butenoate to prepare a highly effective attractant core containing Spodoptera exigua, seal the outlets at both ends of the capillary with electric heating splints, put the capillary into an aluminum foil ziplock bag, seal it and store it in a -20°C refrigerator.

The present invention also provides an application of the high-efficiency attractant composition for broad-legged armyworm in green control of broad-legged armyworm. The attractant core containing the high-efficiency attractant for broad-legged armyworm is placed in a box-type trap, and the trap is placed in an area where broad-legged armyworm needs to be controlled.

Compared with the prior art, the present invention has the following beneficial effects: after adding an appropriate amount of synergistic enhancer to the sex pheromone attractant core of Spodoptera exigua, the present invention can effectively increase the amount of male Spodoptera exigua trapped, effectively interfere with the mating between male and female insects, and reduce the probability of harm to offspring. At the same time, the attractant is environmentally friendly and easy to use, and can be accurately used for insect monitoring, large-scale trapping, and mating interference.

BRIEF DESCRIPTION OF THE DRAWINGS

Figures 1 and 2 show the average number of male insects trapped by different bait formulas, where different letters represent the number of male insects trapped at the 0.05 level.

DETAILED DESCRIPTION

The specific implementation modes of the present invention are described in detail below in conjunction with the accompanying drawings, but it should be understood that the protection scope of the present invention is not limited by the specific implementation modes.

Weigh 0.1 g of sex pheromone of Spodoptera exigua and dissolve it in 1 mL of chlorinated paraffin. Heat it in a water bath until it is completely dissolved to prepare a 100 μg/μL stock solution. Gradient dilutions are used to prepare 5 different concentrations of chlorinated paraffin solutions of sex pheromone, namely 0.001 μg/μL, 0.01 μg/μL, 0.1 μg/μL, 1 μg/μL, and 10 μg/μL. Use a pipette to draw 100 μL of 10 μg/μL chlorinated paraffin solution of sex pheromone and add it to a red polyethylene capillary to prepare a sex pheromone lure. According to a similar method, add cis-11-hexadecene-2-bromopropionate or cis-11-hexadecene-2-butenoate to the sex pheromone lure to prepare a highly efficient attractant lure. Seal the outlets of the capillary at both ends with an electric heating splint, seal it in an aluminum foil ziplock bag and store it in a -20℃ refrigerator.

In order to ensure the release effect, 10-1200 μg of high-efficiency attractant for broad-legged moth was placed in each bait core. In order to further illustrate the feasibility of this implementation, some formulas of the experiment are listed to determine the best formula, as shown in Table 1 and Table 2.

Table 1

formula Cis-11-hexadecene-1-ol (µg) Cis-11-hexadecene-2-bromopropionate (µg) K1 1000 1 K2 1000 10 K3 1000 50 K4 1000 70 K5 1000 100 K6 1000 200 K7 1000 0 K8 0 10

Table 2

formula Cis-11-hexadecene-1-ol (µg) Cis-11-hexadecene-2-butenoate (µg) J1 1000 1 J2 1000 10 J3 1000 50 J4 1000 70 J5 1000 100 J6 1000 200 J7 1000 0 J8 0 100

The experiment was conducted in areas where the broad-legged armyworm was seriously damaged. The traps were box-type traps with a trap spacing of 15 m. The traps were hung on trap poles 1.2-1.6 m high. Recording method: The number of traps in each trap was recorded every three days, and the position of the trap was randomly changed to eliminate the experimental error caused by the position effect. The investigation lasted for 30 days. The experimental results are shown in Tables 3 and 4.

Table 3 and Table 4: Results of trapping tests of different formulations on broad-legged moth

Table 3

Recipe Number Average catch (heads/trap/30 days) K1 15.67±2.85 df K2 62.00±2.52 a K3 40.67±5.49 b K4 29.00±1.15 bcd K5 23.67±2.33 cd K6 22.33±2.85 cd K7 32.67±1.45 bc K8 4.67±0.88 f

Table 4

Recipe Number Average catch (heads/trap/30 days) J1 17.67±1.45 cd J2 23.33±1.52 bc J3 26.67±2.03 bc J4 30.67±3.18 b J5 53.00±4.04 a J6 30.33±2.03 b J7 35.00±2.65 b J8 7.67±1.20 d

From the results in Table 3 and Table 4, it can be seen that the high-efficiency attractant of the broad-legged armyworm of the present invention has a good trapping effect on the broad-legged armyworm, and adding a single type of synergist to the sex pheromone of the broad-legged armyworm can increase the amount of male insects trapped, which is significantly different from the sex pheromone. When a synergist is added, when the content of cis-11-hexadecene-2-bromopropionate is 10 μg, the average trapping amount is significantly higher than the trapping amount of only adding sex pheromone (P < 0.05), and when the content of cis-11-hexadecene-2-butenoate is 100 μg, the trapping amount is significantly higher than the formula of only adding sex pheromone. The results showed that the addition of cis-11-hexadecene-2-bromopropionate or cis-11-hexadecene-2-butenoate had a synergistic effect on the trapping activity of the sex pheromone of Spodoptera exigua. Among them, the trapping effect of formula K2 (containing 1000 μg sex pheromone and 10 μg cis-11-hexadecene-2-bromopropionate) was the best, with an average trapping amount of 62.00±2.52 heads/trap/30 days, and the trapping effect of formula J5 (containing 1000 μg sex pheromone and 100 μg cis-11-hexadecene-2-butenoate) was the best, with an average trapping amount of 53.00±4.04 heads/trap/30 days.

The raw materials of the synergist of broad-legged armyworm provided by the invention are easy to obtain; when a certain amount of the synergist is added, it has an obvious synergistic effect on the sex pheromone of broad-legged armyworm, and can be used to trap broad-legged armyworm in large quantities; at the same time, the prepared high-efficiency attractant core has good stability, the control effect can be maintained for more than 30 days, the specificity is strong, the use is convenient, and the environment is friendly, and it can be accurately applied to insect monitoring, large-scale trapping, and mating interference. It is of great significance for green prevention and control of broad-legged armyworm.

The above disclosure is only a specific embodiment of the present invention, but the present invention is not limited thereto, and any changes that can be conceived by those skilled in the art should fall within the protection scope of the present invention.

Claims (7)

1. A highly effective attractant for Spodoptera exigua, characterized in that the attractant composition comprises a sex pheromone of Spodoptera exigua and a synergist, wherein the sex pheromone is cis-11-hexadecene-1-ol, and the synergist is one of cis-11-hexadecene-2-bromopropionate or cis-11-hexadecene-2-butenoate. 2. An efficient attractant composition for Spodoptera exigua according to claim 1, characterized in that the mass of the sex pheromone cis-11-hexadecene-1-ol of Spodoptera exigua is 1000 μg, the mass of the synergist is 1-1000 μg, and the composition is composed of the following components in mass: 1-100 μg of cis-11-hexadecene-2-bromopropionate or 100-1000 μg of cis-11-hexadecene-2-butenoate. 3. An efficient attractant composition for Spodoptera exigua according to claim 2, characterized in that the mass of the synergist of the Spodoptera exigua is 10-100 μg, and is composed of the following components in mass: 10 μg of cis-11-hexadecene-2-bromopropionate or 100 μg of cis-11-hexadecene-2-butenoate. 4. An attractant core for controlling Spodoptera exigua, characterized in that it contains the high-efficiency attractant composition of Spodoptera exigua according to any one of claims 1 to 3. 5. The method for preparing an attractant for controlling Spodoptera exigua according to claim 4 is characterized in that a chlorinated paraffin solution containing sex pheromone of Spodoptera exigua is first added to a red polyethylene capillary, and then 10 μg of cis-11-hexadecene-2-bromopropionate or 100 μg of cis-11-hexadecene-2-butenoate is added to prepare an attractant containing an efficient attractant for Spodoptera exigua, the outlets at both ends of the capillary are sealed with electric heating splints, and the capillary is placed in an aluminum foil ziplock bag and sealed and stored in a -20°C refrigerator. 6. Use of the high-efficiency attractant for broad-shanked armyworm according to any one of claims 1 to 5 in green control of broad-shanked armyworm. 7. The use of the efficient attractant for broad-shanked armyworm in green control of broad-shanked armyworm according to claim 6 is characterized in that an attractant core containing the efficient attractant for broad-shanked armyworm is placed in a box-type trap, and the trap is placed in an area that needs control.