CN118294190A - Detection system and analysis detection method for circulating tumor cells - Google Patents
Detection system and analysis detection method for circulating tumor cells Download PDFInfo
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- CN118294190A CN118294190A CN202410730850.0A CN202410730850A CN118294190A CN 118294190 A CN118294190 A CN 118294190A CN 202410730850 A CN202410730850 A CN 202410730850A CN 118294190 A CN118294190 A CN 118294190A
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Abstract
The invention relates to the technical field of cell detection, in particular to a detection system and an analysis detection method for circulating tumor cells, which comprises a tissue slice table, wherein a guide rail is fixedly arranged in the middle of the upper surface of the tissue slice table, a slice sampling assembly is arranged between the two guide rails, the slice sampling assembly comprises a sampling storage box, the middle of the sampling storage box is fixedly connected with a plurality of clapboards, one side of each clapboards is fixedly connected with a side baffle, and a cavity is formed by the side baffle, the clapboards and the inner wall of the sampling storage box; the inclined hole has been seted up to the side shield keeping away from sample case inner wall, can utilize the tension of water to expand the section through the setting of section sampling assembly, guarantees sliced integrality, and every section is deposited alone simultaneously, avoids the section to pile up, appears damaging when follow-up sample, and the liquid that slowly flows out from the side shield can drive the section and slowly move to the middle part that sample case and baffle formed the space, is favorable to follow-up section to take out, can also avoid the section to appear damaging simultaneously.
Description
Technical Field
The invention relates to the technical field of cell detection, in particular to a detection system and an analysis detection method for circulating tumor cells.
Background
Tumor cells refer to cells that proliferate abnormally and grow out of control in human tissue, and normally, the growth and division of the cells are strictly regulated to maintain the normal structure and function of the tissue, however, when certain genetic mutations or other abnormalities cause the cells to lose this regulatory mechanism, tumor cell formation occurs.
Tumor cell section detection is a common pathological technique for observing cell morphology, structure and expression of specific proteins in tumor tissues, and is a general procedure of tumor cell section detection:
drawing materials: the material is taken from a patient's tumor tissue sample, typically a tissue specimen obtained by surgery or biopsy.
Fixing: the obtained tumor tissue sample is subjected to fixation treatment, and common fixatives include formalin, acetaldehyde and the like, for the purpose of maintaining the cell morphology and structure.
Dehydration and embedding: the fixed tissue sample is dehydrated and then embedded in paraffin or other embedding agent to allow the tissue to retain its shape when it is fixed in sections.
Slicing: the embedded tissue sample is cut into very thin sections, typically a thickness of a few microns to tens of microns, using a tissue microtome.
Wax block treatment: the cut tissue sections are placed on a slide and the sections are then thermally secured to the slide.
Dyeing: the sections are stained, and common staining methods include hematology staining, immunohistochemical staining, etc., to observe the expression of intracellular nuclei, organelles, and proteins.
And (3) observing by a microscope: the stained sections were placed under a microscope for observation, and cell morphology and structure were observed by different staining methods and magnifications.
Analysis of results: the observed cell morphology, structure and staining results under the microscope were analyzed to diagnose tumor type, evaluate disease condition and guide treatment.
Tumor cell slice detection is one of the common techniques in pathological diagnosis, and can provide important pathological information to help doctors to accurately diagnose and treat tumors.
The patent of the bulletin number CN218994761U of authorizing discloses a prefabricated embedded tissue slicer of cell, including the slicer main part, the bottom of slicer main part is provided with the bottom plate, the collecting vat has been seted up to the inside of bottom plate, and this patent can improve pathology embedded tissue slicer's whole practicality through the design.
Above-mentioned patent is when carrying out the section many times, and the section can pile up at the blade platform, places the section in the liquid through the staff after the section is accomplished, utilizes the tension of liquid to spread, then puts the slide glass again and carry out the next step operation, because the wax stone appears breaking easily, when the staff places the section in water, the problem that the section damaged appears easily, secondly, above-mentioned patent is through manual control section, and sliced degree of consistency error is great, influences subsequent dyeing step after the section, and breakage damage appears easily to the too thin, inconvenient subsequent detection.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a detection system and an analysis detection method for circulating tumor cells, solves the problem that slices are accumulated easily to be damaged in the slicing process, and can also ensure the uniformity of the slices and the detection accuracy.
The technical scheme adopted for solving the technical problems is as follows: the detection system of the circulating tumor cells comprises a tissue slice table, a guide rail is fixedly arranged in the middle of the upper surface of the tissue slice table, a slice sampling assembly is arranged between the two guide rails,
The slicing and sampling assembly comprises a sampling storage box, wherein the middle part of the sampling storage box is fixedly connected with a plurality of clapboards, one side of each clapboard is fixedly connected with a side baffle, and the side baffle, the clapboards and the inner wall of the sampling storage box form a cavity;
the side baffle is provided with an inclined hole far away from the inner wall of the sampling storage box, the inclined hole points to one side far away from the baffle plate, and the inclined hole is obliquely upwards arranged at 15 degrees;
The tissue slice table is characterized in that a rotating wheel is rotatably arranged in the middle of the tissue slice table, the top of the rotating wheel is in contact with the lower surface of the sampling storage box, and the outer wall of the rotating wheel is roughly arranged.
Specifically, the one end that the guide rail is close to tissue section platform middle part is provided with the mounting bracket, mounting bracket middle part fixedly connected with installation diaphragm, the upper surface middle part fixedly connected with telescopic cylinder of installation diaphragm, telescopic cylinder's flexible end slip runs through the installation diaphragm.
Specifically, flexible cylinder fixed pipe is connected with the carriage, the one end fixedly connected with connecting rod of flexible cylinder is kept away from to the carriage, the connecting rod is kept away from carriage one end fixedly connected with section blade.
Specifically, one side that the slicing blade kept away from the guide rail fixedly connected with arc branch pole, the one end fixedly connected with pressurized-water spare that the connecting rod was kept away from to arc branch pole.
Specifically, the water pressing piece comprises a water pressing plate and an outer rubber ring, wherein the water pressing plate is made of hard plastic, the outer rubber ring is fixedly connected to the outer wall of the water pressing plate, and the water pressing plate is matched with the side baffle.
Specifically, the guide rail is close to mounting bracket one end fixedly connected with leading truck, the leading truck is close to one side middle part fixedly connected with installation piece of guide rail, one side that the installation piece is close to the connecting rod rotates and is connected with the transmission shaft, the middle part of leading truck rotates and is connected with the threaded rod, the threaded rod passes through drive belt with the transmission shaft and is connected.
Specifically, the connecting rod is close to the one end middle part rotation that the transmission shaft is connected with one-way board of stirring, the lower fixed surface who stirs the board one-way is connected with reset spring, reset spring is the arc setting, reset spring keeps away from one end fixed connection that one-way stirred the board at the connecting rod lateral wall, the transmission shaft is close to one-way board one end outer wall fixedly connected with of stirring and stirs the gear, stir gear and one-way board cooperation setting of stirring.
Specifically, one side of the unidirectional poking plate is fixedly connected with a limit stop, a limit stop shaft is fixedly connected with the side wall of one end of the connecting rod, which is close to the limit stop, and the limit stop shaft is matched with the limit stop.
Specifically, the guide rail is internally provided with a moving block in a sliding manner, the moving block is in threaded sleeve connection with the outer wall of the threaded rod, the upper surface of the guide rail is provided with a fixing frame, and the moving block is fixedly connected with the lower surface of the fixing frame.
An analytical detection method of a detection system for circulating tumor cells as described above, the method comprising the steps of:
Step one: the agar block is fixed on a fixed frame, a rotating wheel is started, the rotating wheel drives a slice sampling assembly arranged above to move through intermittent motion controlled by a program, then the expansion end of an expansion cylinder is controlled to reciprocate to elongate and shorten, when the expansion end of the expansion cylinder is elongated, the expansion end pushes a sliding frame to move downwards, the sliding frame drives a connecting rod at the bottom to synchronously move, and therefore a slice blade is driven to move downwards to slice the agar block;
Step two: the bottom can enter into the sample case earlier when the section blade is to the section down in, put water in the sample case, because the section is thinner when the section blade enters into the water in the sample case, the section of one side can float on the surface of water, and the section is close to one side of section blade and arranges the below in, before the section contacts the water in the sample case, the arc branch pole of arranging the section blade both sides promotes the water pressing plate and enters into the side shield downwards and extrude the liquid in the side shield, the liquid that flows out slowly in the follow side shield can drive section slow movement to sample case and baffle formation space's middle part, take out the section and carry out analysis and detection.
The invention has the beneficial effects that:
(1) According to the detection system and the analysis detection method for the circulating tumor cells, the slices can be unfolded by using the tension of water through the arrangement of the slice sampling assembly, so that the integrity of the slices is ensured, meanwhile, each slice is stored independently, the accumulation of the slices is avoided, the damage during subsequent sampling is avoided, the slowly-flowing liquid flowing out of the side baffle can drive the slices to slowly move to the middle part of a space formed by the sampling storage box and the partition plate, the subsequent taking out of the slices is facilitated, meanwhile, the damage of the slices can be avoided, the inclined holes are inclined upwards by 15 degrees, and the slices can be slowly pushed to a position far away from the edge of the partition plate by using water flow, so that the slices are facilitated to be taken out by workers.
(2) According to the detection system and the analysis detection method for the circulating tumor cells, when the water pressing plate enters the cavity, pressure is firstly generated on the water surface to press out the water flushing cavity, when the water pressing plate moves too fast, water can press the edge of the water pressing plate to flow upwards when the pressure generated on the opposite side is too high, the water flowing out of the inclined holes is prevented from flowing too much, damage to the slices is avoided, the outer rubber ring can be prevented from moving out of the periphery of the water pressing plate when the water pressing plate descends slowly, the water can flow out of the inclined holes, deformation can occur when the pressure of the outer rubber ring close to one side of the water is too high, the water flows out to relieve pressure, and the slices are further prevented from being damaged due to the too large water flowing.
(3) According to the detection system and the analysis detection method for the circulating tumor cells, the threaded rod is driven to rotate through the transmission belt, and the threaded rod can drive the moving block to slide along the guide rail in the rotating process, so that the agar block of the fixing frame piece is pushed to the side close to the slicing blade, the pushing distance is the same every time, and the slicing uniformity is ensured.
Drawings
The invention will be further described with reference to the drawings and examples.
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is a position diagram of a rotating wheel of the present invention;
FIG. 3 is a schematic view of a guide frame structure according to the present invention;
FIG. 4 is a schematic view of the structure of FIG. 3A according to the present invention;
FIG. 5 is a schematic view of a guide frame structure according to the present invention;
FIG. 6 is a schematic view of the positions of the slicing blade and the connecting rod of the present invention;
FIG. 7 is a schematic view of a water pressing member according to the present invention;
FIG. 8 is a schematic diagram of the flow of water within the cavity of the present invention.
In the figure: 1. a tissue slice station; 12. a mounting frame; 13. mounting a transverse plate; 2. a slice sampling assembly; 21. a sampling storage box; 22. a partition plate; 23. side baffles; 24. an inclined hole; 3. a guide rail; 31. a guide frame; 32. a moving block; 321. a transmission shaft; 322. stirring the gear; 33. a drive belt; 34. a threaded rod; 35. a mounting block; 4. agar blocks; 5. a fixing frame; 61. a telescopic cylinder; 62. a carriage; 63. a connecting rod; 631. a unidirectional toggle plate; 632. a return spring; 633. a limit stop; 634. limiting the baffle shaft; 64. arc branch; 65. a water pressing member; 651. a water pressing plate; 652. an outer rubber ring; 66. a slicing blade; 7. and rotating the wheel.
Detailed Description
The invention is further described in connection with the following detailed description in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the invention easy to understand.
As shown in fig. 1-8, the detection system of circulating tumor cells of the invention comprises a tissue slice table 1, a guide rail 3 is fixedly arranged in the middle of the upper surface of the tissue slice table 1, a slice sampling assembly 2 is arranged between the two guide rails 3,
The slicing and sampling assembly 2 comprises a sampling storage box 21, wherein the middle part of the sampling storage box 21 is fixedly connected with a plurality of baffle plates 22, one side of each baffle plate 22 is fixedly connected with a side baffle plate 23, and the side baffle plates 23, the baffle plates 22 and the inner wall of the sampling storage box 21 form a cavity;
the side baffle plate 23 is provided with an inclined hole 24 far away from the inner wall of the sampling storage box 21, the inclined hole 24 points to one side far away from the baffle plate 22, and the inclined hole 24 is arranged at an upward angle of 15 degrees;
the middle part of the tissue slice table 1 is rotatably provided with a rotating wheel 7, the top of the rotating wheel 7 is contacted with the lower surface of the sampling storage box 21, and the outer wall of the rotating wheel 7 is roughly provided.
The tension through section sampling assembly 2 can utilize the section to expand, guarantees sliced integrality, and every section is deposited alone simultaneously, avoids the section to pile up, appears damaging when follow-up sample.
The slowly flowing liquid flowing out of the side baffle plate 23 can drive the slices to slowly move to the middle part of the space formed by the sampling storage box 21 and the partition plate 22, so that the slices can be taken out later, and meanwhile, the slices can be prevented from being damaged. The inclined holes 24 are inclined upwards by 15 degrees, and the slices can be slowly pushed to a position far away from the edge of the partition plate 22 by utilizing water flow, so that the slices can be taken out by workers.
When cutting, firstly, the agar block is fixed on the fixed frame 5, the rotating wheel 7 is started, the rotating wheel 7 drives the slice sampling assembly 2 arranged above to move through intermittent motion controlled by a program, then the expansion end of the expansion cylinder 61 is controlled to reciprocate to extend and shorten, when the expansion end of the expansion cylinder 61 extends, the expansion end pushes the sliding frame 62 to move downwards, the sliding frame 62 drives the connecting rod 63 at the bottom to synchronously move, and accordingly the slice blade 66 is driven to move downwards to slice the agar block 4.
Specifically, the one end that guide rail 3 is close to tissue section platform 1 middle part is provided with mounting bracket 12, mounting bracket 12 middle part fixedly connected with installation diaphragm 13, the upper surface middle part fixedly connected with telescopic cylinder 61 of installation diaphragm 13, telescopic cylinder 61's flexible end slip runs through installation diaphragm 13.
The mounting frame 12 and the mounting transverse plate 13 play a role in supporting and mounting, and the telescopic cylinder 61 is used as a power source for providing power for continuous slicing.
Specifically, the telescopic cylinder 61 is fixedly connected with a sliding frame 62 through a pipe, one end, away from the telescopic cylinder 61, of the sliding frame 62 is fixedly connected with a connecting rod 63, and one end, away from the sliding frame 62, of the connecting rod 63 is fixedly connected with a slicing blade 66.
When the telescopic end of the telescopic cylinder 61 stretches, the telescopic end pushes the sliding frame 62 to move downwards, the sliding frame 62 drives the connecting rod 63 at the bottom to move synchronously, and accordingly the slicing blade 66 is driven to move downwards to slice the agar block 4.
Specifically, an arc branch 64 is fixedly connected to one side of the slicing blade 66 far away from the guide rail 3, and a water pressing member 65 is fixedly connected to one end of the arc branch 64 far away from the connecting rod 63.
Before the chip contacts the water in the sampling storage box 21, the arc-shaped branches 64 arranged at the two sides of the slicing blade 66 push the water pressing piece 5 downwards into the side baffle plate 23 and press the liquid in the side baffle plate 23 out to generate water flow.
Specifically, the water pressing member 65 includes a water pressing plate 651 and an outer rubber ring 652, the water pressing plate 651 is made of hard plastic, the outer rubber ring 652 is fixedly connected to the outer wall of the water pressing plate 651, and the water pressing plate 651 is adapted to the side baffle 23.
When the water pressing plate 651 enters the cavity, pressure is generated on the water surface firstly to press out the water in the water flushing cavity, when the water pressing plate 651 moves too fast, water can flow upwards at the edge of the water pressing plate 651 when the pressure generated on the opposite side is too large, water flowing out of the inclined holes 24 is prevented from flowing through the water pressing plate, damage to the slices is avoided, the outer rubber ring 652 can be prevented from moving out of the periphery of the water pressing plate 651 when the water pressing plate 651 descends slowly, water can flow out of the inclined holes 24, deformation can occur when the pressure on one side of the outer rubber ring 652 close to the water is too large, and the water flows out to release pressure.
Specifically, guide rail 3 is close to mounting bracket 12 one end fixedly connected with leading truck 31, leading truck 31 is close to one side middle part fixedly connected with installation piece 35 of guide rail 3, one side that installation piece 32 is close to connecting rod 63 rotates and is connected with transmission shaft 321, the middle part of leading truck 31 rotates and is connected with threaded rod 34, threaded rod 34 passes through drive belt 33 with transmission shaft 321 and is connected.
The transmission shaft 321 can drive the threaded rod 34 to synchronously rotate through the transmission belt 33 in the rotating process.
Specifically, the middle part of one end of the connecting rod 63, which is close to the transmission shaft 321, is rotationally connected with a unidirectional stirring plate 631, the lower surface of the unidirectional stirring plate 631 is fixedly connected with a reset spring 632, the reset spring 632 is in an arc shape, one end of the reset spring 632, which is far away from the unidirectional stirring plate 631, is fixedly connected with the side wall of the connecting rod 63, the outer wall of one end of the transmission shaft 321, which is close to the unidirectional stirring plate 631, is fixedly connected with a stirring gear 322, and the stirring gear 322 is matched with the unidirectional stirring plate 631;
One side of the unidirectional stirring plate 631 is fixedly connected with a limit stop 633, a side wall of one end of the connecting rod 63, which is close to the limit stop 633, is fixedly connected with a limit stop shaft 634, and the limit stop shaft 634 is matched with the limit stop 633.
In the downward moving process of the connecting rod 63, the unidirectional stirring plate 631 arranged on one side is driven to move downward, the unidirectional stirring plate 631 contacts with the stirring gear 322 in the downward moving process, the unidirectional stirring plate 631 rotates upward to give way, the return spring 632 is stretched in the process, when the connecting rod 63 moves upward under the pulling of the telescopic cylinder 61 after slicing, the unidirectional stirring plate 631 contacts with the stirring gear 322 and drives the stirring gear 322 to rotate, the stirring gear 322 drives the transmission shaft 321 to synchronously rotate during rotation, the threaded rod 34 is driven to rotate through the transmission belt 33, and the threaded rod 34 can drive the moving block 32 to slide along the guide rail 3 in the rotating process.
Specifically, a moving block 32 is slidably arranged in the guide rail 3, the moving block 32 is in threaded sleeve connection with the outer wall of the threaded rod 34, a fixing frame 5 is arranged on the upper surface of the guide rail 3, and the moving block 32 is fixedly connected with the lower surface of the fixing frame 5.
When the connecting rod 63 is moved upwards under the pulling of the telescopic cylinder 61, the unidirectional stirring plate 631 contacts with the stirring gear 322 and drives the stirring gear 322 to rotate, the stirring gear 322 drives the transmission shaft 321 to synchronously rotate when rotating, the threaded rod 34 is driven to rotate through the transmission belt 33, the threaded rod 34 can drive the moving block 32 to slide along the guide rail 3 in the rotating process, so that the agar block 4 of the fixed frame 5 is pushed to the side close to the slicing blade 66, and the pushing distance is the same every time, thereby ensuring the slicing uniformity.
The invention also provides an analysis and detection method of the detection system of the circulating tumor cells, which comprises the following steps: when slicing, firstly, agar blocks are fixed on the fixing frame 5, the rotating wheel 7 is started, the rotating wheel 7 drives the slicing sampling assembly 2 arranged above to move through intermittent motion controlled by a program, then the telescopic ends of the telescopic cylinders 61 are controlled to reciprocate to extend and shorten, when the telescopic ends of the telescopic cylinders 61 extend, the telescopic ends push the sliding frame 62 to move downwards, the sliding frame 62 drives the connecting rods 63 at the bottom to synchronously move, and accordingly the slicing blades 66 are driven to move downwards to slice the agar blocks 4.
The bottom can enter into the sample case 21 earlier when the section blade 66 is cut into slices downwards, water is put in the sample case 21, because the section is thinner when the section blade 66 enters into the water in the sample case 21, the section of one side can float on the surface of water, and the section is close to one side of section blade 66 and is arranged in the below, before the section contacts the water in the sample case 21, the arc branch bars 64 of placing in the section blade 66 both sides promote the water pressing plate 651 to enter into the side shield 23 downwards and extrude the liquid in the side shield 23, the slow liquid that flows out from the side shield 23 can drive the section to slowly move to the middle part in the sample case 21 and baffle 22 formation space, be favorable to follow-up section take out, can also avoid the section to appear damaging simultaneously.
In the downward moving process of the connecting rod 63, the unidirectional stirring plate 631 arranged on one side is driven to move downward, the unidirectional stirring plate 631 contacts with the stirring gear 322 in the downward moving process, the unidirectional stirring plate 631 rotates upward to give way, the return spring 632 is stretched in the process, when the connecting rod 63 moves upward under the pulling of the telescopic cylinder 61 after slicing is completed, the unidirectional stirring plate 631 contacts with the stirring gear 322 and drives the stirring gear 322 to rotate, the stirring gear 322 drives the transmission shaft 321 to synchronously rotate during rotation, the threaded rod 34 is driven to rotate through the transmission belt 33, the threaded rod 34 can drive the moving block 32 to slide along the guide rail 3 in the rotating process, thereby pushing the fixed frame 5 agar blocks 4 to push to the side close to the slicing blade 66, and the pushing distance is the same every time, so that the slicing uniformity is ensured.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the foregoing examples, and that the foregoing description and description are merely illustrative of the principles of this invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. The detection system of circulating tumor cells, its characterized in that: comprises a tissue slice table (1), a guide rail (3) is fixedly arranged in the middle of the upper surface of the tissue slice table (1), a slice sampling assembly (2) is arranged between the two guide rails (3),
The slicing and sampling assembly (2) comprises a sampling storage box (21), wherein the middle part of the sampling storage box (21) is fixedly connected with a plurality of partition boards (22), one side of each partition board (22) is fixedly connected with a side baffle (23), and the side baffles (23), the partition boards (22) and the inner wall of the sampling storage box (21) form a cavity;
The side baffle (23) is far away from the inner wall of the sampling storage box (21) and is provided with an inclined hole (24), the inclined hole (24) points to one side far away from the baffle plate (22), and the inclined hole (24) is obliquely upwards arranged at 15 degrees;
the tissue slice table is characterized in that a rotating wheel (7) is rotatably arranged in the middle of the tissue slice table (1), the top of the rotating wheel (7) is in contact with the lower surface of the sampling storage box (21), and the outer wall of the rotating wheel (7) is roughly arranged.
2. The system for detecting circulating tumor cells according to claim 1, wherein: one end that guide rail (3) is close to tissue section platform (1) middle part is provided with mounting bracket (12), mounting bracket (12) middle part fixedly connected with installation diaphragm (13), the upper surface middle part fixedly connected with telescopic cylinder (61) of installation diaphragm (13), the flexible end slip of telescopic cylinder (61) runs through installation diaphragm (13).
3. The system for detecting circulating tumor cells according to claim 2, wherein: the telescopic cylinder (61) is fixedly connected with a sliding frame (62) through a pipe, one end, away from the telescopic cylinder (61), of the sliding frame (62) is fixedly connected with a connecting rod (63), and one end, away from the sliding frame (62), of the connecting rod (63) is fixedly connected with a slicing blade (66).
4.A system for detection of circulating tumor cells according to claim 3, wherein: one side of the slicing blade (66) far away from the guide rail (3) is fixedly connected with an arc branch rod (64), and one end of the arc branch rod (64) far away from the connecting rod (63) is fixedly connected with a water pressing piece (65).
5. The system for detecting circulating tumor cells of claim 4, wherein: the water pressing piece (65) comprises a water pressing plate (651) and an outer rubber ring (652), the water pressing plate (651) is made of hard plastic, the outer rubber ring (652) is fixedly connected to the outer wall of the water pressing plate (651), and the water pressing plate (651) is matched with the side baffle plate (23).
6. The system for detecting circulating tumor cells of claim 5, wherein: guide rail (3) are close to mounting bracket (12) one end fixedly connected with leading truck (31), leading truck (31) are close to one side middle part fixedly connected with installation piece (35) of guide rail (3), one side rotation that installation piece (35) is close to connecting rod (63) is connected with transmission shaft (321), the middle part rotation of leading truck (31) is connected with threaded rod (34), threaded rod (34) are connected through driving belt (33) with transmission shaft (321).
7. The system for detecting circulating tumor cells of claim 6, wherein: the one-way toggle plate (631) is rotationally connected to the middle part of one end that connecting rod (63) is close to transmission shaft (321), the lower fixed surface who stirs board (631) is connected with reset spring (632), reset spring (632) are the arc setting, one end fixed connection that one-way toggle plate (631) was kept away from to reset spring (632) is in connecting rod (63) lateral wall, transmission shaft (321) is close to one-way toggle plate (631) one end outer wall fixedly connected with toggle gear (322), toggle gear (322) and one-way toggle plate (631) cooperation setting.
8. The system for detecting circulating tumor cells of claim 7, wherein: one side of the unidirectional stirring plate (631) is fixedly connected with a limit stop (633), one end side wall of the connecting rod (63) close to the limit stop (633) is fixedly connected with a limit stop shaft (634), and the limit stop shaft (634) is matched with the limit stop (633).
9. The system for detecting circulating tumor cells of claim 8, wherein: the guide rail (3) is provided with a moving block (32) in a sliding mode, the moving block (32) is in threaded sleeve connection with the outer wall of the threaded rod (34), the upper surface of the guide rail (3) is provided with a fixing frame (5), and the moving block (32) is fixedly connected with the lower surface of the fixing frame (5).
10. The analytical detection method of a detection system for circulating tumor cells according to claim 9, wherein: the method comprises the following steps:
Step one: the agar block is fixed on a fixed frame (5), a rotating wheel (7) is started, the rotating wheel (7) drives a slice sampling assembly (2) arranged above to move through intermittent motion controlled by a program, then the expansion end of an expansion cylinder (61) is controlled to reciprocate to extend and shorten, when the expansion end of the expansion cylinder (61) extends, the expansion end pushes a sliding frame (62) to move downwards, the sliding frame (62) drives a connecting rod (63) at the bottom to synchronously move, so that a slice blade (66) is driven to move downwards, and the agar block (4) is sliced;
Step two: the bottom can enter into in sample case (21) earlier when section blade (66) cut into slices downwards, put water in sample case (21), because the section is thinner when section blade (66) enters into the water in sample case (21), the section of one side can float on the surface of water, and the section is close to one side of section blade (66) and is arranged in the below, before the section contacts the water in sample case (21), arc branch pole (64) of arranging the section blade (66) both sides promote water pressing board (651) and enter into in side shield (23) downwards and extrude the liquid in side shield (23), the slow outflow liquid that flows out in from side shield (23) can drive section and slowly move to the middle part in sample case (21) and baffle (22) formation space, take out the section and carry out analysis and detection.
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| CN202410730850.0A CN118294190A (en) | 2024-06-06 | 2024-06-06 | Detection system and analysis detection method for circulating tumor cells |
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| CN202410730850.0A CN118294190A (en) | 2024-06-06 | 2024-06-06 | Detection system and analysis detection method for circulating tumor cells |
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