CN118243918A - Light path module for dry fluorescent immunodetection - Google Patents
Light path module for dry fluorescent immunodetection Download PDFInfo
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- CN118243918A CN118243918A CN202410670895.3A CN202410670895A CN118243918A CN 118243918 A CN118243918 A CN 118243918A CN 202410670895 A CN202410670895 A CN 202410670895A CN 118243918 A CN118243918 A CN 118243918A
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- 230000003287 optical effect Effects 0.000 claims abstract description 166
- 230000005284 excitation Effects 0.000 claims abstract description 26
- 238000006243 chemical reaction Methods 0.000 claims abstract description 25
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 13
- 230000008033 biological extinction Effects 0.000 claims abstract description 8
- 238000009434 installation Methods 0.000 claims description 6
- 210000001503 joint Anatomy 0.000 claims description 3
- 238000003825 pressing Methods 0.000 claims description 3
- 238000013461 design Methods 0.000 abstract description 5
- 238000001514 detection method Methods 0.000 description 3
- 238000003018 immunoassay Methods 0.000 description 3
- 238000012417 linear regression Methods 0.000 description 3
- 239000004005 microsphere Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 229910052693 Europium Inorganic materials 0.000 description 2
- 101100537532 Rattus norvegicus Tnni3 gene Proteins 0.000 description 2
- -1 europium ions Chemical class 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- 102000017011 Glycated Hemoglobin A Human genes 0.000 description 1
- 108010014663 Glycated Hemoglobin A Proteins 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229910052747 lanthanoid Inorganic materials 0.000 description 1
- 150000002602 lanthanoids Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
- G01N33/723—Glycosylated haemoglobin
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses an optical path module for dry fluorescent immunodetection, which comprises an excitation light optical path component, a plurality of optical lenses, a photoelectric conversion module, a diaphragm block and an extinction tail cone, wherein the optical lenses comprise a right optical lens, a middle optical lens, a left optical lens, a lens and a square optical lens, the excitation light optical path component and the photoelectric conversion module are horizontally placed after being vertical, the middle optical lens is arranged in the middle position between the excitation light optical path component and the photoelectric conversion module, the right optical lens is arranged at the corresponding position of the excitation light optical path component on one side of the middle optical lens, the diaphragm block and the left optical lens are arranged at the corresponding position of the photoelectric conversion module on the other side of the middle optical lens, the bottom of a lower cover of the optical path module is provided with the lens component consisting of the lens and the square optical lens, and a reagent strip is placed below the lens component. By improving the structure of the light path, the height and the volume of the whole light path module are reduced, and the stacking design of portable products is facilitated.
Description
Technical Field
The invention relates to an optical path module, in particular to an optical path module for dry fluorescent immunodetection.
Background
The light source component is used for dry fluorescent immunoassay and the principle of the dry fluorescent immunoassay: forming immune complex by the object to be detected in the sample and the fluorescent labeled antibody, and respectively solidifying in a detection area and a quality control area through a chromatography process; when the reagent card reaches the detection area, the light wave emitted by the excitation light source of the analyzer irradiates the detection area and the quality control area of the reagent card, the solidified fluorescent immune antigen-antibody complex is excited, the light wave emitted by the fluorescent material is collected by the signal acquisition board and converted into an electric signal, the intensity of the electric signal is closely related to the concentration and the quantity of fluorescent molecules, and the concentration of the analyte in the sample to be detected is calculated according to the intensity of the feedback electric signal.
The existing light source component is high in height and large in size due to the light path structural design, and finally the height and the size of the analyzer are increased, so that the light source component is not suitable for being used in portable handheld equipment.
Disclosure of Invention
In order to solve the problem of large volume caused by unreasonable optical path structural design, the invention provides an optical path module for dry fluorescent immunodetection, which has small volume and is convenient to hold.
The invention provides the following technical scheme:
the utility model provides a light path module for dry-type fluorescence immunoassay, including the excitation light optical path subassembly, a plurality of optical lenses, photoelectric conversion module, diaphragm piece and extinction tail cone, a plurality of optical lenses include right side optical lens, middle optical lens, left side optical lens, square optical lens, the level is placed behind the perpendicular excitation light optical path subassembly and the photoelectric conversion module, middle optical lens is installed to intermediate position between excitation light optical path subassembly and the photoelectric conversion module, the excitation light optical path subassembly of intermediate optical lens one side corresponds the department and sets up right side optical lens, the photoelectric conversion module of intermediate optical lens opposite side corresponds the department and sets up diaphragm piece and left side optical lens, right side optical lens, intermediate optical lens, left side optical lens is installed between light path module upper cover and light path module lower cover, the lens subassembly that comprises lens and square optical lens is installed to the bottom of light path module lower cover, reagent strip is placed to the corresponding position below the lens subassembly.
Further, an optical path is formed between the optical lenses.
Further, a first light path is formed between the right optical lens and the middle optical lens, a second light path is formed between the middle optical lens and the square optical lens, a third light path is formed among the square optical lens, the lens and the reagent strip window, and a fourth light path is formed between the square optical lens and the left optical lens.
Further, the left optical lens and the right optical lens are respectively arranged on the photoelectric conversion module and the excitation light path component through respective gaskets.
Further, the lens is mounted on the lower cover of the light path module through the elastic pad and the lens mounting pressing block.
Further, the square optical lens is arranged in the installation groove where the upper cover of the optical path module is in butt joint with the lower cover of the optical path module.
Further, the extinction tail cone is fixed in a groove of the lower cover of the light path module.
Compared with the prior art, the invention has the beneficial effects that: the excitation light path component and the photoelectric conversion module are horizontally placed after being vertical, the middle optical lens is arranged in the middle between the excitation light path component and the photoelectric conversion module, the left optical lens and the right optical lens are arranged on two sides of the middle optical lens, an optical path is formed between the optical lenses, and the height and the volume of the whole light path module are reduced by improving the structure of the optical path, so that the stacking design of portable products is facilitated.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention.
FIG. 2 is a schematic view of an optical path according to the present invention.
FIG. 3 is a graph showing the comparison of troponin correlation according to the present invention.
FIG. 4 is a graph showing the correlation of glycosylated hemoglobin according to the present invention.
In the figure: 1. the photoelectric conversion module, 2, the light path module upper cover, 3, the light path module lower cover, 4, right side optical lens, 5, middle optical lens, 6, left side optical lens, 7, lens, 8, square optical lens, 9, gasket, 10, spring pad, 11, lens installation briquetting, 12, excitation light path component, 13, diaphragm piece, 14, extinction tail cone, 15, mounting screw, 16, round hole gasket, 17, packing ring, 18, first light path, 19, second light path, 20, third light path, 21, reagent strip, 22, fourth light path.
Description of the embodiments
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-2, the optical path module for dry fluorescent immunodetection of the present invention includes an excitation light optical path component 12, a plurality of optical lenses, a photoelectric conversion module 1, a diaphragm block 13 and an extinction tail 14, wherein the plurality of optical lenses include a right optical lens 4, a middle optical lens 5, a left optical lens 6, a lens 7 and a square optical lens 8, the excitation light optical path component 12 and the photoelectric conversion module 1 are horizontally placed after being vertical, the middle optical lens 5 is installed at a middle position between the excitation light optical path component 12 and the photoelectric conversion module 1, the right optical lens 4 is disposed at a position corresponding to the excitation light optical path component 12 on one side of the middle optical lens 5, the diaphragm block 13 and the left optical lens 6 are disposed at a position corresponding to the photoelectric conversion module 1 on the other side of the middle optical lens 5, the right optical lens 4, the middle optical lens 5 and the left optical lens 6 are installed between the optical path module upper cover 2 and the optical path module lower cover 3, a lens component composed of the lens 7 and the square optical lens 8 is installed at a bottom of the optical path module lower cover 3, a reagent strip is placed at a corresponding position below the lens component, and an optical path is formed between the optical lenses.
A first light path 18 is formed between the right optical lens 4 and the intermediate optical lens 5, a second light path 19 is formed between the intermediate optical lens 5 and the square optical lens 8, a third light path 20 is formed between the square optical lens 8, the lens 7 and the reagent strip 21 window, and a fourth light path 22 is formed between the square optical lens 8 and the left optical lens 6.
The left optical lens 6 and the right optical lens 4 are respectively arranged on the photoelectric conversion module 1 and the excitation light path component 12 through respective gaskets 9.
The lens 7 is mounted on the optical path module lower cover 3 through the spring pad 10 and the lens mounting press block 11. The extinction tail cone 14 is fixed in the groove of the light path module lower cover 3.
The right optical lens 4, the middle optical lens 5, the left optical lens 6, the diaphragm block 13 and the placing gasket 9 are respectively placed in the installation area of the lower cover 3 of the optical path module, the upper cover 2 of the optical path module is covered, and the upper cover 2 of the optical path module and the lower cover 3 of the optical path module are locked through the installation screw 15 at the top of the upper cover;
The excitation light path component 12 and the photoelectric conversion module 1 are arranged on the upper cover and the lower cover of the assembled light path module; the lens 7 and the spring pad 10 are placed in the lower light path module cover 3, the lens 7 is fixed by using the lens mounting pressing block 11, the square optical lens 8 is placed in a mounting groove where the upper light path module cover 2 and the lower light path module cover 3 are in butt joint, and the square optical lens 8 is locked by using the mounting screw 15 and the gasket 17.
Working principle:
Excitation light is emitted through the excitation light path component 12, passes through the first light path 18, passes through the right optical lens 4, is reflected to the second light path 19 through the middle optical lens 5, is reflected to the third light path 20 through the square optical lens 8, is incident to the reagent strip 21 through the lens 7, the excited light generated by fluorescent microspheres (the fluorescent microspheres are prepared by wrapping europium ions with polystyrene microspheres, the europium ions are lanthanoids) on the reagent strip 21 is incident to the third light path 20, is reflected to the fourth light path 22 through the lens 7 by the square optical lens 8, enters the photoelectric conversion module 1 after passing through the middle optical lens 5, the left optical lens 6 and the diaphragm block 13, and is processed by the photoelectric conversion module 1 to obtain the concentration of an item to be detected in a sample. The structure of the optical path is improved, so that the height and the volume of the whole optical path module are reduced, and the optical path module is suitable for manufacturing portable handheld instruments.
Linear regression analysis: the cTnI, HAB1c project fits a linear regression equation, a linear regression scatter plot, with the base egg Getein1100 as y, to the instrument (BK 120) test results x of the present invention, as shown in fig. 3-4.
Table 1 cTnI item data sheet
TABLE 2 HAB1c project data sheet
Summary of the verification:
The results of the tests of the present invention and the bid comparison meet the reagent performance requirements as shown in tables 1 and 2.
Therefore, in order to solve the problem of larger volume caused by unreasonable optical path structural design, the invention reduces the whole volume of the optical path module by reasonably arranging a plurality of optical paths, is convenient to hold, and does not influence the test result of the invention.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. An optical path module for dry fluorescent immunodetection, characterized in that: including excitation light optical path component (12), a plurality of optical lenses, photoelectric conversion module (1), diaphragm piece (13) and extinction tail cone (14), a plurality of optical lenses include right side optical lens (4), middle optical lens (5), left side optical lens (6), lens (7), square optical lens (8), the perpendicular back level of excitation light optical path component (12) and photoelectric conversion module (1) is placed, middle optical lens (5) are installed to intermediate position between excitation light optical path component (12) and photoelectric conversion module (1), the excitation light optical path component (12) of middle optical lens (5) one side corresponds the department and sets up right side optical lens (4), the photoelectric conversion module (1) of middle optical lens (5) opposite side corresponds the department and sets up diaphragm piece (13) and left side optical lens (6), right side optical lens (4), middle optical lens (5), left side optical lens (6) are installed between optical path module upper cover (2) and optical path module lower cover (3), the bottom installation of optical path module lower cover (3) comprises lens (7) and optical lens component (8), the reagent strip is placed to the corresponding position below the lens component.
2. The optical path module for dry fluorescent immunodetection of claim 1, wherein: an optical path is formed between the optical lenses.
3. The optical path module for dry fluorescent immunodetection of claim 2, wherein: a first light path (18) is formed between the right optical lens (4) and the middle optical lens (5), a second light path (19) is formed between the middle optical lens (5) and the square optical lens (8), a third light path (20) is formed between the square optical lens (8), the lens (7) and the window of the reagent strip (21), and a fourth light path (22) is formed between the square optical lens (8) and the left optical lens (6).
4. The optical path module for dry fluorescent immunodetection of claim 1, wherein: the left optical lens (6) and the right optical lens (4) are respectively arranged on the photoelectric conversion module (1) and the excitation light path component (12) through respective gaskets (9).
5. The optical path module for dry fluorescent immunodetection of claim 1, wherein: the lens (7) is arranged on the lower cover (3) of the light path module through the elastic pad (10) and the lens installation pressing block (11).
6. The optical path module for dry fluorescent immunodetection of claim 1, wherein: the square optical lens (8) is arranged in a mounting groove where the upper cover (2) of the optical path module and the lower cover (3) of the optical path module are in butt joint.
7. The optical path module for dry fluorescent immunodetection of claim 1, wherein: the extinction tail cone (14) is fixed in a groove of the lower cover (3) of the light path module.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410670895.3A CN118243918B (en) | 2024-05-28 | 2024-05-28 | Light path module for dry fluorescent immunodetection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410670895.3A CN118243918B (en) | 2024-05-28 | 2024-05-28 | Light path module for dry fluorescent immunodetection |
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| Publication Number | Publication Date |
|---|---|
| CN118243918A true CN118243918A (en) | 2024-06-25 |
| CN118243918B CN118243918B (en) | 2024-08-16 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410670895.3A Active CN118243918B (en) | 2024-05-28 | 2024-05-28 | Light path module for dry fluorescent immunodetection |
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0067963A2 (en) * | 1981-05-27 | 1982-12-29 | Boehringer Ingelheim International GmbH | Object holder as part of a photometric or fluorimetric device |
| EP1998167A2 (en) * | 2007-06-01 | 2008-12-03 | Samsung Electronics Co., Ltd. | Fluorescence detecting module for microreaction and fluorescence detecting system having the same |
| CN105759025A (en) * | 2016-03-01 | 2016-07-13 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Photoelectric detection system and time-resolved fluorescence immunoassay analyzer |
| CN206684041U (en) * | 2017-02-21 | 2017-11-28 | 安迈斯（北京）科技有限公司 | The burnt low veiling glare fluoroscopic examination optical system of one kind copolymerization |
| CN207396349U (en) * | 2017-07-11 | 2018-05-22 | 扬州千代科技有限公司 | A kind of colloidal gold chromatographic card interpretoscope |
| CN109085352A (en) * | 2018-09-26 | 2018-12-25 | 北京乐普医疗科技有限责任公司 | A kind of Optical devices of immunochromatographiassays assays instrument |
| CN215116301U (en) * | 2020-12-24 | 2021-12-10 | 中元汇吉生物技术股份有限公司 | Optical detection system and analysis device of immunofluorescence analyzer |
| CN215448994U (en) * | 2021-08-20 | 2022-01-07 | 四川新健康成生物股份有限公司 | Fluorescence detection optical system |
| CN219302274U (en) * | 2022-12-14 | 2023-07-04 | 广州万孚生物技术股份有限公司 | Fluorescence detection device |
| CN118050505A (en) * | 2024-04-15 | 2024-05-17 | 南京佰抗生物科技有限公司 | Multiple combined inspection fluorescence immunochromatography detection card |
-
2024
- 2024-05-28 CN CN202410670895.3A patent/CN118243918B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0067963A2 (en) * | 1981-05-27 | 1982-12-29 | Boehringer Ingelheim International GmbH | Object holder as part of a photometric or fluorimetric device |
| EP1998167A2 (en) * | 2007-06-01 | 2008-12-03 | Samsung Electronics Co., Ltd. | Fluorescence detecting module for microreaction and fluorescence detecting system having the same |
| CN105759025A (en) * | 2016-03-01 | 2016-07-13 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Photoelectric detection system and time-resolved fluorescence immunoassay analyzer |
| CN206684041U (en) * | 2017-02-21 | 2017-11-28 | 安迈斯（北京）科技有限公司 | The burnt low veiling glare fluoroscopic examination optical system of one kind copolymerization |
| CN207396349U (en) * | 2017-07-11 | 2018-05-22 | 扬州千代科技有限公司 | A kind of colloidal gold chromatographic card interpretoscope |
| CN109085352A (en) * | 2018-09-26 | 2018-12-25 | 北京乐普医疗科技有限责任公司 | A kind of Optical devices of immunochromatographiassays assays instrument |
| CN215116301U (en) * | 2020-12-24 | 2021-12-10 | 中元汇吉生物技术股份有限公司 | Optical detection system and analysis device of immunofluorescence analyzer |
| CN215448994U (en) * | 2021-08-20 | 2022-01-07 | 四川新健康成生物股份有限公司 | Fluorescence detection optical system |
| CN219302274U (en) * | 2022-12-14 | 2023-07-04 | 广州万孚生物技术股份有限公司 | Fluorescence detection device |
| CN118050505A (en) * | 2024-04-15 | 2024-05-17 | 南京佰抗生物科技有限公司 | Multiple combined inspection fluorescence immunochromatography detection card |
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| CN118243918B (en) | 2024-08-16 |
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