CN1182241C - Microbial silicon magnesium calcium fertilizer and its production method - Google Patents

Microbial silicon magnesium calcium fertilizer and its production method Download PDF

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Publication number
CN1182241C
CN1182241C CNB021148775A CN02114877A CN1182241C CN 1182241 C CN1182241 C CN 1182241C CN B021148775 A CNB021148775 A CN B021148775A CN 02114877 A CN02114877 A CN 02114877A CN 1182241 C CN1182241 C CN 1182241C
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China
Prior art keywords
bacterial classification
magnesium calcium
silicon magnesium
microbial
fertilizer
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Expired - Lifetime
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CNB021148775A
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CN1367243A (en
Inventor
飚 何
何飚
黄恩雄
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Jinkuizi Environmental Biotechnology (International) Co., Ltd.
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Foshan Jinkuizi Plant Nutriment Co Ltd
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Priority to CNB021148775A priority Critical patent/CN1182241C/en
Publication of CN1367243A publication Critical patent/CN1367243A/en
Priority to PCT/CN2003/000103 priority patent/WO2003074680A1/en
Priority to AU2003208253A priority patent/AU2003208253A1/en
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Publication of CN1182241C publication Critical patent/CN1182241C/en
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D3/00Calcareous fertilisers

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Fertilizers (AREA)

Abstract

The present invention relates to a microbial silicon magnesium calcium fertilizer and a production method thereof. An organic substance is used as a life carrier of a microbial WH flora; after the organic substance, silex powder, and magnesium calcium stone powder are mixed and fermented, active silicon magnesium calcium fertilizer is manufactured. After being put in soil, the silicon magnesium calcium fertilizer becomes soluble salt because of a microbial function and is absorbed by a plant for generating the following obvious effects: (1), the neutralization of soil acidity and an improvement on a soil structure; (2), the enhancement of the haulm tissue of crops, the promotion of plant cell division, and the pachynsis of roots, stems and leaves; (3), chlorophyll increase; the activation of the activity of various enzymes in a plant body; the enhancement of photosynthesis and the capability of operating photosynthesis products; the promotion of plant growth; (4), the reinforcement of the capability of resisting diseases and insect pests; (5), the enhancement of the yield and the quality of crops.

Description

A kind of microbial silicon magnesium calcium fertilizer
Technical field
The present invention relates to the silicon magnesium calcium fertilizer technical field, particularly a kind of microbial silicon magnesium calcium fertilizer.
Background technology
The rural area wide geographic area all is single use inorganic fertilizer to farm crop for a long time, ignore the use of fertilizer, add rainwater and irrigation water leaching and wash away, elements such as the calcium in the soil, silicon are easy to be flushed away loss, and iron, aluminium are built up and increased P in soil H value slant acidity, the soil colloid that goes down for a long time is destroyed, cause soil compaction, lose the effect of preserve moisture and fertility, influence the farm crop normal growth.
Because population increase, the per capita cultivated land subtracts greatly, and the modern technologies reinforcement is asked for increasing agricultural-food to the soil, execute the three elements chemical fertilizer for asking to save trouble, and the comprehensive farm manure of nutrient is used less day by day more.If things go on like this, quite a few nutritive element is unable to make ends meet in the farmland, causes many plants to show all nutritional deficiency symptoms.Not only the essential micro-constant of plant lacks, and moderate-element and beneficial element magnesium, calcium, silicon also often wane, because of climatic zone of living in and soil parent material decision, this performance that wanes of certain areas soil is more outstanding, many farm crop is improved the influence of yield improvement quality and more sees obviously.Magnesium (Mg) is chlorophyllous central metallic ions, and magnesium deficiency just can not form chlorophyll, and photosynthesis just can not produce.Magnesium still is the moiety of plurality of enzymes in the plant materials, and their physiological function is related to many significant process of carbohydrate, fat and proteinic metabolism and Conversion of energy.Calcium (Ca) is the important composition element of plant cell wall, and the transportation of calcium pair cell proper splitting, photosynthate, that meristematic tissue enlivens differential growth is indispensable, to promoting the growth of root and cauline leaf, and makes it to have certain mechanical strength and plays an important role.Competent calcium supply also can reduce the physiological disturbance of fruit, improves fruit quality and storage tolerance.People are relatively familiar to the effect of magnesium, calcium, but just fuzzyyer to the effect understanding of silicon element (Si).Silicon in the soil, the overwhelming majority exists with the crystalline mineral form, combines closely with other element, and water insoluble, the utmost point embarrasses plant absorbing.Crop mainly absorbs silicon ion and the molecular state silicic acid that is dissolved in the water, and minority available state silicon compound is then easily washed away loss by leaching in the soil.For southern sour earth, the validity of calcium, magnesium was sharply reduced with the reduction of pH value, and damp and hot weather condition make the matrix that forms soil be low silicon sexupara matter (SiO again 2/ R 2O 3<2) lack validity silicon.Even also there is the phenomenon that lacks effective silicon in calcareous soil.As solubility in acid fertilizer, the acidulous material that no doubt reliable plant root exudation goes out draw the silicon element from fertilizer, but solubleness is limited, absorb often inadequate, particularly more happiness silicon plant to the silicon cellulose content, as paddy rice gramineous, sugarcane, wheat class, corn, jowar, bamboo, and piece root tubers, watermelon, peanut or the like, plant under the ground condition in that effectively silicon is insufficient, strengthen supply intensity, further raising yield improvement quality is had great significance silicon.Under brute force that this production presses for promotes, utilize microorganism, be equipped with suitable material, act on the ore powder of siliceous, magnesium, calcium, the development work of biological activity silicon magnesium calcium fertilizer that discharges the available state silicon of volume just becomes developing direction from now on.
Summary of the invention
The technical problem to be solved in the present invention is how to make silicon magnesium calcium ion in the silicon magnesium calcium fertilizer easily by plant absorbing.
Technical solution is such: a kind of microbial silicon magnesium calcium fertilizer, described microorganism are bacillus polymyxa (CGMCC0395.1) WH1 bacterial classifications by name; Subtilis (CGMCC0395.2) is called the WH2 bacterial classification; Bacillus brevis (CGMCC0395.3) is called the WH3 bacterial classification; Bacillus licheniformis (CGMCC0395.4) is called the WH4 bacterial classification; Sulphur look bacillus (CGMCC0395.5) is called the WH5 bacterial classification; Bacillus polymyxa (CGMCC0395.6) is called the WH6 bacterial classification; It also contains ground silica, calcium magnesite powder and rice bran.The proportioning that described microbial silicon magnesium calcium fertilizer includes bacterial classification is (weight percent): (1) WH1 bacterial classification 14-24, (2) WH2 bacterial classification 6-24, (3) WH3 bacterial classification 8-25, (4) WH4 bacterial classification 7-23, (5) WH5 bacterial classification 7-23, (6) WH6 bacterial classification 13-30.The manufacture method of mentioned microorganism silicon magnesium calcium fertilizer comprises spawn culture and preparation:
(1) described WH1 bacterial classification, WH2 bacterial classification, WH3 bacterial classification, WH4 bacterial classification, WH5 bacterial classification, WH6 bacterial classification are mixed composition WH flora by proportioning;
(2) WH flora secondary medium prescription (weight percent):
Rice bran 80, soyflour 16, milk powder 2, sugar 1, water 1;
(3) second class inoculum is cultivated: the WH colony culture medium is mixed by above-mentioned prescription mix thoroughly earlier, normal temperature is inoculation WH flora down, 35-60 ℃ of substratum temperature, PH7.0, incubation time 7-12 days, cultivate and obtain the active chaff bacterium that ripe back obtains the WH flora, contain at least 2 hundred million of viable counts/gram;
(4) with ground silica, calcium magnesite powder, active chaff bacterium, by following prescription mixed fermentation 7-12 days active SiMgCa fertilizer (weight percent):
Ground silica 20-50, calcium magnesite powder 30-70, active chaff bacterium 10-20.
The present invention is a kind of biological activity silicon magnesium calcium fertilizer that utilizes biotechnology to be developed into, utilize the WH bacterial classification that insoluble silicon magnesium calcium constituent is resolved into the silicon magnesium calcium constituent of solubility, make it to become the active SiMgCa fertilizer that can directly utilize and can supply plant absorbing with the mixing with soil dissolving.
The present invention has following remarkable beneficial effect: the effect that (1) has significant volume increase and improves quality; (2) energy sweetening of the soil acidity, the structure of improving the soil; (3) strengthen stem stalk tissue, promote plant cell division, make the root, stem and leaf plumpness; (4) increase chlorophyll, the vigor of various enzymes in the activated plant body, the ability that improves photosynthesis and transport photosynthate; (5) strengthen disease-resistant, insect pest, lodging tolerance.
Embodiment
By following embodiment the present invention is further elaborated.
Embodiment 1:
The main component of a kind of microbial silicon magnesium calcium fertilizer of the present invention is:
WH1 bacterial classification, WH2 bacterial classification, WH3 bacterial classification, WH4 bacterial classification, WH5 bacterial classification, WH6 bacterial classification and ground silica, calcium magnesite powder, rice bran.
Mentioned microorganism, WH1 bacterial classification are bacillus polymyxa (Bacillus Polymyxa), are preserved in CGMCC, preserving number 0359.1; The WH2 bacterial classification is subtilis (BacillusSubtilis), is preserved in CGMCC, preserving number 0359.2; The WH3 bacterial classification is bacillus brevis (Bacillus Brevis), is preserved in CGMCC, preserving number 0359.3; The WH4 bacterial classification is Bacillus licheniformis (Bacillus Licheniformis), is preserved in CGMCC, preserving number 0359.4; The WH5 bacterial classification is sulphur look tyrothricin (Bacillus Sulfurenm), is preserved in CGMCC, preserving number 0359.5; The WH6 bacterial classification is bacillus polymyxa (Bacillus Polymyxa), is preserved in CGMCC, preserving number 0359.6;
The proportioning that this microbial silicon magnesium calcium fertilizer includes bacterial classification is (weight percent):
(1) WH1 bacterial classification: 24 (2) WH2 bacterial classifications: 24 (3) WH3 bacterial classifications: 8 (4) WH4 bacterial classifications: 7 (5) WH1 bacterial classifications: 16 (6) WH6 bacterial classifications: 21.
The manufacture method of this microbial silicon magnesium calcium fertilizer comprises the preparation of spawn culture, and its processing step is:
(1) above-mentioned WH1 bacterial classification, WH2 bacterial classification, WH3 bacterial classification, WH4 bacterial classification, WH5 bacterial classification, WH6 bacterial classification are formed the WH flora by the said ratio uniform mixing;
(2) WH flora secondary medium prescription is (weight percent):
Rice bran 80, soyflour 16, milk powder 2, sugar 1, water 1;
(3) second class inoculum is cultivated: WH flora secondary medium is mixed by above-mentioned prescription mix thoroughly earlier, inoculate the WH flora at normal temperatures, 60 ℃ of substratum temperature, PH7.0, incubation time 7 days is cultivated the active chaff bacterium that ripe back obtains the WH flora, contains viable count and can reach 2,000,000,000/gram;
(4) ground silica, calcium magnesite powder, active chaff bacterium were pressed following prescription mixing stack retting 7 days, packing becomes active SiMgCa fertilizer:
Ground silica 50, calcium magnesite powder 30, active chaff bacterium 20.
Embodiment 2:
The proportioning of each composition of microbial silicon magnesium calcium fertilizer of present embodiment and each parameter and example one difference are:
1, this microbial silicon magnesium calcium fertilizer proportioning of including bacterial classification is (weight percent):
WH1 bacterial classification 8 WH2 bacterial classifications 14
WH3 bacterial classification 25 WH4 bacterial classifications 23
WH1 bacterial classification 7 WH6 bacterial classifications 13.
2, second class inoculum substratum: temperature is 47 ℃, and incubation time 9 days contains viable count and can reach 1,500,000,000/gram.
3, ground silica, calcium magnesite powder, active chaff bacterium were mixed pile fermentation 9 days by following prescription (weight percent), packing becomes microbial silicon magnesium calcium fertilizer:
Ground silica 20, calcium magnesite powder 70, active chaff bacterium 10.
Each parameter of the proportioning of all the other each compositions and processing step are with example one.
Embodiment 3:
The proportioning of each composition of microbial silicon magnesium calcium fertilizer of present embodiment and each parameter and example one difference are:
1, this microbial silicon magnesium calcium fertilizer proportioning of including bacterial classification is (weight percent):
(1) WH1 bacterial classification 14 (2) WH2 bacterial classifications 6
(3) WH3 bacterial classification 14 (5) WH4 bacterial classifications 13
(4) WH1 bacterial classification 23 (6) WH6 bacterial classifications 30.
2, second class inoculum substratum: temperature is 47 ℃, and incubation time 9 days contains viable count and can reach 1,500,000,000/gram.
3, ground silica, calcium magnesite powder, active chaff bacterium were mixed pile fermentation 12 days by following prescription (weight percent), packing becomes microbial silicon magnesium calcium fertilizer:
Ground silica 35, calcium magnesite powder 50, active chaff bacterium 15.
The proportioning parameter of all the other each compositions and processing step are with example one.
Use the actual effect of this biological activity silicon magnesium calcium fertilizer:
1, Beixiang, county, Lechang, Shaoguan, Guangdong Province town, this microbial silicon magnesium calcium fertilizer that had four peasant households to use Gaoming Jinkuizi Plant Nutrient Co. Ltd. to provide in 1999 topdresses as secondary to water chestnut, makes first grade increase by 34.2%, and mu increases income 202.2 yuan.
2, Fu Ru Shan of brilliant city, Guangdong Province Xian District peasant household, on conventional fertilizer application basis enrich of the present invention active SiMgCa fertilizer 30kg/ mu to potato year February in December, 1998-99, and potato piece per unit area yield comparison as a result is according to increasing production more than 50%.
3, the Li Da of Heshui town, brilliant city, Guangdong Province peasant household is strong, Pachyrhizua angulatus test in 1999, and on the basis of 50 kilograms of conventional mu sealing fertilizers, mu enriches active SiMgCa fertilizer of the present invention, and the result compares according to root of kudzu vine volume increase 6.4%, and wherein first grade increases by 9.7%.
4, clay fertilizer master station in Guangdong Province's used active SiMgCa fertilizer of the present invention to paddy rice, peanut test in 1999, reduced rice sheath blight disease 4.7-28.3%, and alleviated peanut iron rust disease.

Claims (3)

1, a kind of microbial silicon magnesium calcium fertilizer is characterized in that: described microorganism is bacillus polymyxa CGMCC0395.1, WH1 bacterial classification by name; Subtilis CGMCC0395.2, WH2 bacterial classification by name; Bacillus brevis CGMCC0395.3, WH3 bacterial classification by name; Bacillus licheniformis CGMCC0395.4, WH4 bacterial classification by name; Sulphur look tyrothricin CGMCC0395.5, WH5 bacterial classification by name; Bacillus polymyxa CGMCC0395.6, WH6 bacterial classification by name; It also contains ground silica, calcium magnesite powder and rice bran.
2, microbial silicon magnesium calcium fertilizer according to claim 1 is characterized in that: the proportioning that described microbial silicon magnesium calcium fertilizer includes bacterial classification is (weight percent):
(1) WH1 bacterial classification 14-24 (2) WH2 bacterial classification 6-24
(3) WH3 bacterial classification 8-25 (4) WH4 bacterial classification 7-23
(5) WH5 bacterial classification 7-23 (6) WH6 bacterial classification 13-30.
3, the manufacture method of microbial silicon magnesium calcium fertilizer according to claim 1 comprises spawn culture and preparation, it is characterized in that:
(1) the described WH1 bacterial classification of claim 1, WH2 bacterial classification, WH3 bacterial classification, WH4 bacterial classification, WH5 bacterial classification, WH6 bacterial classification are mixed composition WH flora by proportioning;
(2) WH flora secondary medium prescription (weight percent):
Rice bran 80, soyflour 16, milk powder 2, sugar 1, water 1;
(3) second class inoculum is cultivated: the WH colony culture medium is mixed by above-mentioned prescription mix thoroughly earlier, normal temperature is inoculation WH flora down, 35-60 ℃ of substratum temperature, PH7.0, incubation time 7-12 days, cultivate and obtain the active chaff bacterium that ripe back obtains the WH flora, contain at least 2 hundred million of viable counts/gram;
(4) with ground silica, calcium magnesite powder, active chaff bacterium, by following prescription mixed fermentation 7-12 days active SiMgCa fertilizer (weight percent):
Ground silica 20-50, calcium magnesite powder 30-70, active chaff bacterium 10-20.
CNB021148775A 2002-02-09 2002-02-09 Microbial silicon magnesium calcium fertilizer and its production method Expired - Lifetime CN1182241C (en)

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CNB021148775A CN1182241C (en) 2002-02-09 2002-02-09 Microbial silicon magnesium calcium fertilizer and its production method
PCT/CN2003/000103 WO2003074680A1 (en) 2002-02-09 2003-01-30 Microbial silicon magnesium calcium fertilizer and its production method
AU2003208253A AU2003208253A1 (en) 2002-02-09 2003-01-30 Microbial silicon magnesium calcium fertilizer and its production method

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Application Number Priority Date Filing Date Title
CNB021148775A CN1182241C (en) 2002-02-09 2002-02-09 Microbial silicon magnesium calcium fertilizer and its production method

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Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1182241C (en) * 2002-02-09 2004-12-29 佛山金葵子植物营养有限公司 Microbial silicon magnesium calcium fertilizer and its production method
CN100361937C (en) * 2003-09-26 2008-01-16 中国科学院沈阳应用生态研究所 Method for producing multifunctional organic fertilizer of microbe
CN101759500B (en) * 2010-01-05 2013-03-13 爱沃(北京)农业技术有限公司 Mineral fertilizer containing complex microbial community and preparation method thereof
CN103408352B (en) * 2013-07-08 2015-04-22 陕西雅枫菌业科技有限公司 Low-carbon composite biological organosilicon magnesium potassium fertilizer
CN104628473A (en) * 2015-01-30 2015-05-20 烟台恩倍得化工科技有限公司 Silicon type organic acid soil conditioner and preparation thereof
CN111302853A (en) * 2020-02-16 2020-06-19 金葵子环保生物科技(国际)有限公司 Microbial phosphorus-potassium fertilizer and preparation method thereof

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* Cited by examiner, † Cited by third party
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CN1191854A (en) * 1998-02-25 1998-09-02 北京乐泰然经济技术咨询有限责任公司 Composite microbial fertilizer and its production process
CN1203900A (en) * 1998-06-10 1999-01-06 北京农乐肥料有限责任公司 Method for industrial production of organic fertilizer
CN1099393C (en) * 1999-11-08 2003-01-22 高明金葵子植物营养有限公司 Microbe preparation for decaying straw or stalk of crops and its preparing process
CN1182241C (en) * 2002-02-09 2004-12-29 佛山金葵子植物营养有限公司 Microbial silicon magnesium calcium fertilizer and its production method

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AU2003208253A1 (en) 2003-09-16
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Granted publication date: 20041229