A kind of microbial silicon magnesium calcium fertilizer and manufacture method thereof
Technical field
The present invention relates to silicon magnesium calcium fertilizer technical field, particularly microbial silicon magnesium calcium fertilizer and manufacture method thereof.
Background technology
The rural area wide geographic area all is single use inorganic fertilizer to farm crop for a long time, ignore the use of fertilizer, add rainwater and irrigation water leaching and wash away, elements such as the calcium in the soil, silicon are easy to be flushed away loss, and iron, aluminium are built up and increased P in soil H value slant acidity, the soil colloid that goes down for a long time is destroyed, cause soil compaction, lose the effect of preserve moisture and fertility, influence the farm crop normal growth.
Because population increase, the per capita cultivated land subtracts greatly, and the modern technologies reinforcement is asked for increasing agricultural-food to the soil, execute the three elements chemical fertilizer for asking to save trouble, and the comprehensive farm manure of nutrient is used less day by day more.If things go on like this, quite a few nutritive element is unable to make ends meet in the farmland, causes many plants to show all nutritional deficiency symptoms.Not only the essential micro-constant of plant lacks, and moderate-element and beneficial element magnesium, calcium, silicon also often wane, because of climatic zone soil parent material decision of living in, this performance that wanes of certain areas soil is more outstanding, many farm crop is improved the influence of yield improvement quality and more sees obviously.Magnesium (Mg) is chlorophyllous central metallic ions, and magnesium deficiency just can not form chlorophyll, and photosynthesis just can not produce.Magnesium still is the moiety of plurality of enzymes in the plant materials, and their physiological function is related to many significant process of carbohydrate, fat and proteinic metabolism and Conversion of energy.Calcium (Ca) is the important composition element of plant cell wall, and the transportation of calcium pair cell proper splitting, photosynthate, that meristematic tissue enlivens differential growth is indispensable, to promoting the growth of root and cauline leaf, and makes it to have certain mechanical strength and plays an important role.Competent calcium supply also can reduce the physiological disturbance of fruit, improves fruit quality and storage tolerance.People are relatively familiar to the effect of magnesium, calcium, but just fuzzyyer to the effect understanding of silicon element (Si).Silicon in the soil, the overwhelming majority exists with the crystalline mineral form, combines closely with other element, and water insoluble, the utmost point embarrasses plant absorbing.Crop mainly absorbs silicon ion and the molecular state silicic acid that is dissolved in the water, and minority available state silicon compound is then easily washed away loss by leaching in the soil.For southern sour earth, the validity of calcium, magnesium was sharply reduced with the reduction of pH value, and damp and hot weather condition make the matrix that forms soil be low silicon sexupara matter (SiO again
2/ R
2O
3<2) lack validity silicon.Even also there is the phenomenon that lacks effective silicon in calcareous soil.As solubility in acid fertilizer, the acidulous material that no doubt reliable plant root exudation goes out draw the silicon element from fertilizer, but solubleness is limited, absorb often inadequate, particularly more happiness silicon plant to the silicon cellulose content, as paddy rice gramineous, sugarcane, wheat class, corn, jowar, bamboo, and piece root tubers, watermelon, peanut or the like, plant under the ground condition in that effectively silicon is insufficient, strengthen supply intensity, further raising yield improvement quality is had great significance silicon.Under brute force that this production presses for promotes, utilize microorganism, be equipped with suitable material, act on the ore powder of siliceous, magnesium, calcium, the development work of biological activity silicon magnesium calcium fertilizer that discharges the available state silicon of volume just becomes developing direction from now on.
Summary of the invention
The technical problem to be solved in the present invention is how to make silicon magnesium calcium ion in the silicon magnesium calcium fertilizer easily by plant absorbing.
Technical solution is such: a kind of microorganism of making silicon magnesium calcium fertilizer, described microorganism comprise bacillus polymyxa (CGMCC0395.1) WH1 bacterial classification by name; (CGMCC0395.2 is called the WH2 kind to subtilis; Bacillus brevis (CGMCC0395.3) is called the WH3 bacterial classification; Bacillus licheniformis (CGMCC0395.4) is called the WH4 bacterial classification; Sulphur look bacillus (CGMCC0395.5) is called the WH5 bacterial classification; Bacillus polymyxa (CGMCC0395.6) is called the WH6 bacterial classification.A kind of microbial silicon magnesium calcium fertilizer, its main component is WH1 bacterial classification, WH2 bacterial classification, WH3 bacterial classification, WH4 bacterial classification, WH5 bacterial classification, WH6 bacterial classification and ground silica calcium, calcium magnesite powder, rice bran, the proportioning that this microbial silicon magnesium calcium fertilizer includes bacterial classification is (weight percent): (1) WH1 bacterial classification 14-24, (2) WH2 bacterial classification 6-24, (3) WH3 bacterial classification 8-25, (4) WH4 bacterial classification 7-23, (5) WH5 bacterial classification 7-23, (6) WH6 bacterial classification 13-30.A kind of manufacture method of microbial silicon magnesium calcium fertilizer comprises spawn culture and preparation:
(1) described WH1 bacterial classification, WH2 bacterial classification, WH3 bacterial classification, WH4 bacterial classification, WH5 bacterial classification, WH6 bacterial classification are mixed composition WH flora by proportioning;
(2) WH flora secondary medium prescription (weight percent):
Rice bran 80, soyflour 16, milk powder 2, sugar 1, water 1;
(3) second class inoculum is cultivated: the WH colony culture medium is mixed by above-mentioned prescription mix thoroughly earlier, normal temperature is inoculation WH flora down, 35-60 ℃ of substratum temperature, PH7.0, incubation time 7-12 days, cultivate and obtain the active chaff bacterium that ripe back obtains the WH flora, contain at least 2 hundred million of viable counts/gram;
(4) with ground silica, calcium magnesite powder, active chaff bacterium, by following prescription mixed fermentation 7-12 days active SiMgCa fertilizer (weight percent):
Ground silica 20-50, calcium magnesite powder 30-70, active chaff bacterium 10-20.
The present invention is a kind of biological activity silicon magnesium calcium fertilizer that utilizes biotechnology to be developed into, utilize the WH bacterial classification that insoluble silicon magnesium calcium constituent is resolved into the silicon magnesium calcium constituent of solubility, make it to become the active SiMgCa fertilizer that can directly utilize and can supply plant absorbing with the mixing with soil dissolving.
The present invention has following remarkable beneficial effect: the effect that (1) has significant volume increase and improves quality; (2) energy sweetening of the soil acidity, the structure of improving the soil; (3) strengthen stem stalk tissue, promote plant cell division, make the root, stem and leaf plumpness; (4) increase chlorophyll, the vigor of various enzymes in the activated plant body, the ability that improves photosynthesis and transport photosynthate; (5) strengthen disease-resistant, insect pest, lodging tolerance.
Embodiment
By following embodiment the present invention is further elaborated.
Embodiment 1:
The main component of a kind of microbial silicon magnesium calcium fertilizer of the present invention is:
WH1 bacterial classification, WH2 bacterial classification, WH3 bacterial classification, WH4 bacterial classification, WH5 bacterial classification, WH6 bacterial classification and ground silica, calcium magnesite powder, rice bran.
Mentioned microorganism, WH1 bacterial classification are bacillus polymyxa (Bacillus Po1ymyxa), are preserved in CGMCC, preserving number 0359.1; The WH2 bacterial classification is subtilis (BacillusSubtilis), is preserved in CGMCC, preserving number 0359.2; The WH3 bacterial classification is bacillus brevis (Bacillus Brevis), is preserved in CGMCC, preserving number 0359.3; The WH4 bacterial classification is Bacillus licheniformis (Bacillus Licheniformis), is preserved in CGMCC, preserving number 0359.4; The WH5 bacterial classification is sulphur look tyrothricin (Bacillus Sulfurenm), is preserved in CGMCC, preserving number 0359.5; The WH6 bacterial classification is bacillus polymyxa (Bacillus Polymyxa), is preserved in CGMCC, preserving number 0359.6;
The proportioning that this microbial silicon magnesium calcium fertilizer includes bacterial classification is (weight percent):
(1) WH1 bacterial classification: 24 (2) WH2 bacterial classifications: 24 (3) WH3 bacterial classifications: 8 (4) WH4 bacterial classifications: 7 (5) WH1 bacterial classifications: 16 (6) WH6 bacterial classifications: 21.
The manufacture method of this microbial silicon magnesium calcium fertilizer comprises the preparation of spawn culture, and its processing step is:
(1) above-mentioned WH1 bacterial classification, WH2 bacterial classification, WH3 bacterial classification, WH4 bacterial classification, WH5 bacterial classification, WH6 bacterial classification are formed the WH flora by the said ratio uniform mixing;
(2) WH flora secondary medium prescription is (weight percent):
Rice bran 80, soyflour 16, milk powder 2, sugar 1, water 1;
(3) second class inoculum is cultivated: WH flora secondary medium is mixed by above-mentioned prescription mix thoroughly earlier, inoculate the WH flora at normal temperatures, 60 ℃ of substratum temperature, PH7.0, incubation time 7 days is cultivated the active chaff bacterium that ripe back obtains the WH flora, contains viable count and can reach 2,000,000,000/gram;
(4) ground silica, calcium magnesite powder, active chaff bacterium were pressed following prescription mixing stack retting 7 days, packing becomes active SiMgCa fertilizer:
Ground silica 50, calcium magnesite powder 30, active chaff bacterium 20.
Embodiment 2:
The proportioning of each composition of microbial silicon magnesium calcium fertilizer of present embodiment and each parameter and example one difference are:
1, this microbial silicon magnesium calcium fertilizer proportioning of including bacterial classification is (weight percent):
WH1 bacterial classification 8 WH2 bacterial classifications 14
WH3 bacterial classification 25 WH4 bacterial classifications 23
WH1 bacterial classification 7 WH6 bacterial classifications 13.
2, second class inoculum substratum: temperature is 47 ℃, and incubation time 9 days contains viable count and can reach 1,500,000,000/gram.
3, ground silica, calcium magnesite powder, active chaff bacterium were mixed pile fermentation 9 days by following prescription (weight percent), packing becomes microbial silicon magnesium calcium fertilizer:
Ground silica 20, calcium magnesite powder 70, active chaff bacterium 10.
Each parameter of the proportioning of all the other each compositions and processing step are with example one.
Embodiment 3:
The proportioning of each composition of microbial silicon magnesium calcium fertilizer of present embodiment and each parameter and example one difference are:
1, this microbial silicon magnesium calcium fertilizer proportioning of including bacterial classification is (weight percent):
(1) WH1 bacterial classification 14 (2) WH2 bacterial classifications 6
(3) WH3 bacterial classification 14 (5) WH4 bacterial classifications 13
(4) WH1 bacterial classification 23 (6) WH6 bacterial classifications 30.
2, second class inoculum substratum: temperature is 47 ℃, and incubation time 9 days contains viable count and can reach 1,500,000,000/gram.
3, ground silica, calcium magnesite powder, active chaff bacterium were mixed pile fermentation 12 days by following prescription (weight percent), packing becomes microbial silicon magnesium calcium fertilizer:
Ground silica 35, calcium magnesite powder 50, active chaff bacterium 15.
The proportioning parameter of all the other each compositions and processing step are with example one.
Use the actual effect of this biologically active silicon magnesium calcium fertilizer:
1, Shaoguan City of Guangdong Province Lechang County Beixiang town had four peasant households to use brilliant golden certain herbaceous plants with big flowers to plant in 1999 This microbial silicon magnesium calcium fertilizer that thing nutrition Co., Ltd provides topdresses as secondary to water chestnut, and primes is increased Add 34.2%, mu increases income 202.2 yuan.
2, Fu Ru Shan of brilliant city, Guangdong Province Xian District peasant household, year February in December, 1998-99 is to potato Enrich active SiMgCa fertilizer 30kg/ mu of the present invention on the conventional fertilizer application basis, as a result potato piece per unit area yield comparison According to increasing production more than 50%.
3, the Li Da of Heshui town, brilliant city, Guangdong Province peasant household is strong, and Pachyrhizua angulatus test in 1999 is executed at conventional mu On the basis that composite fertilizer is 50 kilograms, mu enriches active SiMgCa fertilizer of the present invention, as a result the contrast root of kudzu vine Volume increase 6.4%, wherein primes increases by 9.7%.
4, clay fertilizer master station in Guangdong Province's used activated silica of the present invention to paddy rice, peanut test in 1999 Magnesium calcium fertilizer reduces rice sheath blight disease 4.7-28.3%, and alleviates the peanut iron rust.