CN118177148A

CN118177148A - Method for cultivating multi-embryo mutton sheep suitable for agriculture and animal husbandry by using Xinjiang local ewes

Info

Publication number: CN118177148A
Application number: CN202410462275.0A
Authority: CN
Inventors: 杨华; 杨永林; 魏建军; 余乾; 张宾; 张文喆
Original assignee: Xinjiang Academy of Agricultural and Reclamation Sciences
Current assignee: Xinjiang Academy of Agricultural and Reclamation Sciences
Priority date: 2024-04-17
Filing date: 2024-04-17
Publication date: 2024-06-14

Abstract

The invention discloses a method for cultivating multi-embryo mutton sheep suitable for agriculture and animal husbandry by using Xinjiang local ewes. Belonging to the technical field of animal breeding. On the basis of genetic evaluation of growth and development traits and reproductive performance of multi-embryo sakura and evaluation of general disease resistance of the alemtai sheep, a conventional breeding method is combined with a molecular Marker Assisted Selection (MAS) technology to establish a method for breeding a new strain of Xinjiang local multi-embryo mutton sheep, and the new strain of multi-embryo mutton sheep is bred. The Xinjiang multi-embryo mutton sheep bred by the method has the advantages of high lamb production rate, good meat production performance, fast growth and development, strong adaptability and the like, is suitable for house feeding and fattening in the Xinjiang semi-farming semi-pasturing area, accords with the development trend of the Xinjiang sheep industry, and meets the requirements of the Xinjiang sheep breeding industry.

Description

Method for cultivating multi-embryo mutton sheep suitable for agriculture and animal husbandry by using Xinjiang local ewes

Technical Field

The invention relates to the technical field of animal breeding, in particular to a method for breeding multi-embryo mutton sheep suitable for agriculture and animal husbandry by using Xinjiang local ewes.

Background

The reproductive trait is an important economic trait of sheep, and the reproductive capacity can directly influence the development and economic benefit of sheep raised in a large-scale shed, and is closely related to the large-scale, industrialized and sustainable development of modern sheep raising industry. Most sheep have the characteristics of single-embryo, seasonal oestrus, one embryo in one year, one lamb in one embryo and low reproduction rate; only a few sheep have high fertility and have the characteristics of one-time multiple lambs, premature sexual maturity and perennial oestrus, so that the reproductive performance of sheep is greatly influenced. With the continuous development of intensive sheep raising, how to improve sheep reproductive performance is a key to obtaining better economic benefit for sheep raising industry.

Xinjiang is located on the abdomen of the sub-European continents, far from the ocean, spans 15 latitudes from north to south and 23 longitudes from east to west, and forms a drought and unique natural geographic environment. The interior topography is complex and the climate is changeable. The device has a mountain with a high mountain range, a vast desert and gobi with a vast area, and has an endless basin plains. The climates of Xinjiang are various in types, the difference between the south and north climates is obvious, the south and north climates are respectively warm temperate zone and warm temperate zone continental climate, the four seasons are clear, the winter is cold and summer hot, the drought is less rainy, and the climates are typical arid and semiarid regions, and the difference of precipitation space distribution is obvious. In recent years, special mutton sheep such as SAFUKE sheep, duPoisson sheep and the like with high growth performance are introduced from abroad and abroad, and Hu sheep with high fertility are poor in adaptability in Xinjiang, so that the morbidity and mortality of lambs are high, and the excellent production performance cannot be fully exerted.

In summary, how to provide a cultivation method of high-quality multi-embryo mutton sheep adapting to Xinjiang ecological environment and meeting market demands is a problem to be solved by those skilled in the art.

Disclosure of Invention

In view of the above, the invention provides a method for cultivating multi-embryo mutton sheep suitable for agriculture and animal husbandry by using Xinjiang local ewes.

In order to cultivate high-quality multi-embryo mutton sheep adapting to the ecological environment of Xinjiang and meeting the market demands, the invention fully exerts the production performances of growth and development, meat quality and high fertility, utilizes the strong adaptability advantage of the variety resources of the Xinjiang local aletai sheep, combines a sheep reproductive character FecB gene marker assisted selection method and a general disease resistance IDO1 gene marker assisted selection method to strengthen sheep fertility and adaptive breeding, and breeds new multi-embryo mutton sheep strains with high lamb production rate, good meat production performance, fast growth and development and strong adaptability so as to meet the demands of the Xinjiang vast agriculture and animal husbandry areas on the improved variety of the multi-embryo mutton sheep and the breeding of the multi-embryo mutton sheep in different breeding areas.

In order to achieve the above purpose, the present invention adopts the following technical scheme:

A method for cultivating multi-embryo mutton sheep suitable for agriculture and animal husbandry by using Xinjiang local ewes comprises the following steps:

(1) Taking an alexan sheep with FecB gene++ genotype and IDO1 gene AA genotype as female parents, taking a multi-embryo sakufick sheep with FecB gene BB genotype and IDO1 gene AA genotype as male parents, and hybridizing to obtain F1 multi-alalank sheep;

(2) Taking an F1 multiple-alae sheep with FecB gene B+ genotype and IDO1 gene AA genotype as female parents, taking a multiple-fetid SAFOG sheep with FecB gene BB genotype and IDO1 gene AA genotype as male parents, and hybridizing to obtain an F2 multiple-alae sheep;

(3) Taking F2 multiple-sheep with FecB gene BB and B+ genotype and IDO1 gene AA genotype as female parent, taking multiple-embryo SAFOX sheep with FecB gene BB genotype and IDO1 gene AA genotype as male parent, and hybridizing to obtain F3 multiple-sheep;

(4) And (3) transversely fixing at least four generations of F3 multiple-animal rams with FecB gene BB genotype and IDO1 gene AA genotype, wherein the generation of F3 multiple-animal rams with FecB gene BB and B+ genotype and IDO1 gene AA genotype is selected and remained, and a new strain of the multiple-animal mutton sheep is obtained.

Sheep reproductive traits are complex traits that are affected by a number of genetic and environmental factors that together affect and control the reproductive process, and since 1980 researchers have begun screening for major genes that control sheep ovulation rate and lambing rate, some of which have been identified as having a great effect on reproductive traits. Wherein the FecB gene is a major gene of sheep ovulation number and lambing number, and the gene is a dominant gene, and the phenotype accords with a Mendelian genetic separation mode. FecB gene is caused by autosomal mutation, has additive effect on ovulation rate of sheep, one copy gene of ewe can increase ovulation number by 1.5, two copy genes increase ovulation number by 3.0, and average number of lambs per fetus is increased by 1.0-1.5 due to increase of ovulation rate. Sheep FecB gene is actually bone morphogenetic protein IB receptor (BMPR-IB) gene, A/G single base mutation occurs in 746 position of the gene coding region, glutamine at 249 th position is mutated into arginine (Q-R), phenotype is changed, lambing number of sheep is increased, and 249R is proved to be FecB allele of sheep multiple embryo character. Through the detection of FecB genes, molecular breeding of high-fertility sheep can be realized, so as to promote the breeding process of the multi-sheep.

The multi-embryo safucus sheep is a new multi-embryo mutton sheep strain which is bred by adopting a conventional breeding technology and an FecB gene marker auxiliary selection technology at the national academy of agricultural reclamation in 2002, takes the safucus sheep with high production performance abroad as a terminal male parent, takes domestic high-fertility Hu sheep as a female parent, and is bred into the multi-embryo safucus sheep strain suitable for the production of agricultural and pastoral areas in China through seed selection and matching, cross fixing and breeding improvement for 12 years. The strain has the characteristics of high fertility, good early maturing performance, fast growth and development and high meat production performance. Compared with Hu sheep, the meat performance of the bred multiple-embryo safuck sheep is obviously improved, and the lambing rate reaches 180-200%. Through detection, three genotypes BB, B+ and++ of BMPR-IB gene A746G mutation exist in the multiple-fetopropion sheep population.

The Arietai sheep is used as one of Xinjiang coarse wool sheep varieties and is mainly distributed in places such as northern foot of Tianshan in Xinjiang, southern foot of Arietai mountain, tacheng and the like, and is suitable for living in northern south Xinjiang through a long breeding process due to special natural conditions of local climate. The alemta sheep has the advantages of coarse feeding resistance, strong adaptability and disease resistance, but has the defects of slow growth and development, low fertility and unsatisfactory meat performance, and can not adapt to modern large-scale and standardized housing-raising modes. Through detection, BMPR-IB gene A746G mutation is not existed in the Altai sheep population, namely, only++ genotype exists.

Indoleamine 2,3 dioxygenase (indoleamine-2, 3-diogenase, IDO) is an enzyme with heme in cells, is the only enzyme outside the liver that can catalyze the epoxidation cleavage of indole in tryptophan molecules, and is the rate-limiting enzyme for catabolism along the canine uric acid pathway. IDO is widely distributed in extrahepatic tissues of humans and other mammals and expressed at low levels in the normal state, with a significant increase in IDO expression during inflammation or infection. Early team studies demonstrated that genotype effects caused by mutations in the G1072A site of the coding region of the IDO1 gene are very significantly correlated with IFN- α, IL-2 and IL-12 levels in sheep peripheral blood (P < 0.01); multiple comparison results show that the IFN-alpha and IL-2 levels of the AA and AG genotype individuals are extremely higher than those of the GG genotype individuals (P < 0.01), and the IL-12 levels of the AA genotype individuals are extremely higher than those of the AG and GG genotype individuals (P < 0.01). By detecting IDO1 gene G1072A mutation sites of multiple sheep groups such as SAFOG sheep, multiple-embryo SAFOG sheep, kazakhstan sheep, alemtai sheep, hu sheep and the like, the sites have three genotypes of AA, AG and GG in different sheep groups, and by detecting IDO1 gene G1072A mutation sites, a breeding group carrying the AA genotype is constructed, the group shows good adaptability and viability in grazing and house feeding in the alemtai area of Xinjiang, and shows better resistance than individuals with AG and GG genotypes in resisting infection of mycoplasma pneumoniae pathogens of sheep, and the infection positive rate and death rate of the AA genotype lamb are respectively 21.88% and 15.63% lower than those of AG genotype and 36.46% and 31.25% lower than those of GG genotype. Therefore, in sheep adaptability or general disease resistance molecular breeding, sheep populations are divided into three types according to IDO1 genotypes, AA genotype individuals are selected for grouping, and therefore sheep adaptability or general disease resistance molecular marker assisted selection breeding is achieved.

According to the multi-gene polymerization breeding of the BMPR-IB gene A746G mutation and the IDO1 gene G1072A mutation sites, BB and B+ genotypes of the BMPR-IB gene A746G mutation sites and AA genotype individuals of the IDO1 gene G1072A mutation sites are selected for grouping, so that sheep individuals with high fertility and good adaptability can be selected.

In the steps (1), (2) and (3), a male goat which has no genetic defect, is healthy, has an average daily gain of more than 250g in the growing period, has an adult body weight of more than 120kg, has a slaughter rate of more than 50% and has a offspring-female goat lambing rate of more than 200% is selected as a male parent.

Further, in the step (1), a female sheep which has no genetic defect, is healthy, has a lambing rate of more than 105%, has an adult body weight of more than 60kg and has a lamb survival rate of more than 98% is selected as a female parent.

In the step (2), a ewe which has no genetic defect, is healthy, has a lambing rate of more than 140%, has a weaning body weight of more than 28kg, has a periyears body weight of more than 55kg, has a tail length of less than 13cm and has a tail circumference of less than 50cm is selected as a female parent.

In the step (3), a ewe which has no genetic defect, is healthy, has a lambing rate of more than 170%, has a weaning body weight of more than 28kg, has a perigee body weight of more than 55kg, has a tail length of less than 8cm and has a tail circumference of less than 21cm is selected as a female parent.

Further, in the step (4), a ram which has no genetic defect, is healthy, has no horn, has a birth weight of more than 3.2kg, has a weaning weight of more than 30kg, has a year of life of more than 75kg, has an adult weight of more than 110kg, has a slaughter rate of more than 50%, and has a offspring-to-ewe lambing rate of more than 200% is selected as a male parent.

Further, in the step (4), the female sheep which has no genetic defect, is healthy, has no horn, has a birth weight of more than 3kg, has a weaning weight of more than 28kg, has a perigee weight of more than 55kg, has an adult weight of more than 60kg, has a lamb production rate of more than 185% and has a slaughter rate of more than 48.5% is selected as the female parent.

The breeding aim is to breed the hornless multi-embryo mutton sheep. Because the male parent multiple-embryo safuck sheep has no horns, the female parent Altai sheep has horns in part, and the filial generation has horns in part. The sheep in long angle is fighting and is put up, and the occupied feeding space is large and difficult to manage. Therefore, the breeding of the mutton sheep is mainly to breed the polled variety.

Further, in the step (4), the average lambing rate of the new strain of the multi-sheep is greater than 200%, the average primary weights of the male lambs and the female lambs are 3.28kg and 3.11kg respectively, the average weights of the male lambs and the female lambs at the age of 4 months are 36.52kg and 35.37kg respectively, the average weights of the male lambs and the female lambs at the age of 12 months are 76.25kg and 56.58kg respectively, and the average weights of the adult ram and the female sheep are 111.52kg and 60.16kg respectively.

Compared with the prior art, the invention has the beneficial effects that: on the basis of genetic evaluation of growth and development traits, reproductive performance and adaptability of individuals, the method utilizes a conventional breeding method in combination with a molecular marker auxiliary selection technology, adopts a polygene polymerization breeding technology system, selects a breeding strain (line) of multiple-embryo safuck with healthy constitution, rapid growth and development, high carcass meat production performance, high fertility and good adaptability to form a male parent basic group, and selects a Xinjiang local ewe aletai sheep with healthy constitution, good multiple-embryo performance and good adaptability to form a female parent basic group, so that a new strain of the multiple-embryo mutton sheep with high meat production performance, high fertility and strong adaptability can be cultivated. The average lambing rate of the new strain of the warp-born ewes is more than 200 percent, the average birth weight of the lambs is 3.2kg, the average daily gain of the lambs of 0 to 90 days old is increased by 42.87 percent compared with that of the aletai lambs, the average weight of the lambs of 4 months old is 35.94kg, the average weight of the rams of 12 months old is 76.25kg, and the average weight of the adult rams is 111.52kg. The carcass quality is good, the slaughtering rate of the male lambs of 4 months old is 50.85%, the muscle color is dark red, and the fat is milky white. The cultivated new line has strong environmental adaptability to northern and southern Xinjiang, and is suitable for grazing, semi-grazing and housing breeding in pasture areas and agriculture and grazing staggered areas.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present invention, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic view of the cultivation route of the present invention.

Detailed Description

The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

The required medicament is a conventional experimental medicament and is purchased from a commercial channel;

the test methods not mentioned are conventional test methods and will not be described in detail herein.

In the following examples, the detection of FecB gene refers to "Taqman-MGB probe-based kit and method for detecting sheep BMPR-IB gene A746G mutation, patent number: ZL201710444692.2, yang Hua, yang Yonglin, li Liangyuan, lu Shouliang, yang Hanyu celluloid. Detection of IDO1 gene refers to "a molecular marker method for predicting general disease resistance of sheep", patent of invention, patent number: zl201410498852. X, yang Hua, yang Yonglin, zhong Fagang, zhao Wenjuan, kang Lichao).

Example 1

The cultivation site is a Xinjiang agricultural reclamation academy of sciences, and the sheep farm is located between 44 DEG 15 '43' -44 DEG 19 '13' of North latitude, 85 DEG 59 '12' -86 DEG 08 '13' of east longitude, the middle section of the northern foot of the mountain, the Golgi, the Guerban and the Goodyear desert south margin. The altitude is 450.8 meters, which belongs to the continental climate in the temperate zone, the precipitation is little, the drying is long and severe cold in winter, and the summer is short and hot. The frost-free period is 168-171 days, the average air temperature is 25.1-26.1 ℃, the highest temperature is 42.1 ℃, the lowest temperature is-41.8 ℃, the average annual precipitation is 213 mm, and the annual evaporation capacity is 1537 mm.

1. selection of male parent:

The method comprises the steps of identifying and carrying out genetic evaluation on the body type appearance, growth development and reproductive performance of a multi-embryo saxifrage sheep, adopting FecB gene marker auxiliary selection, IDO1 gene marker auxiliary selection, optimal linear unbiased prediction (BLUP) and genetic evaluation method of the growth performance to select seeds, wherein the selected rams are required to carry FecB gene BB genotype and IDO1 gene AA genotype, have no genetic defects, have a healthy and growing period average daily gain of more than 250g, have an adult body weight of more than 120kg, have a slaughter rate of more than 50%, have a offspring parent lambing rate of more than 200% and are used for constructing a male ram with a BLUP breeding value calculated by an optimal linear unbiased estimation method and ranked 10.

Body shape and body weight selection criteria for multiple-carcass sajoram: healthy, without genetic defects and history of major disease onset. The head and the four limbs are black, and the trunk is white heterogeneous hair. Middle physique, firm constitution, symmetrical structure, big ear, big eyes with spirit, long neck, deep and wide thick, wide and flat fat, wide and deep chest, long and thin tail. The front body is plump, the back body has developed muscles, the back waist is long, wide and straight, the ribs are well opened, the limbs are strong, the hoofs are firm, the body is in a long barrel shape, and the back driver is in an inverted U shape. The muscles above the wrist joint and the flying joint of the hind limb of the ram are well attached. The testis has good development and moderate size, and is bilateral symmetry. The average daily gain in the breeding period is greater than 250g, the adult weight is more than 120kg, the height is more than 85cm, the body length is more than 110cm, the chest circumference is more than 120cm, the tube circumference is more than 11cm, the slaughter rate (5 rams of the same age and weight are selected for sampling slaughter, and the sampling sample reflects the slaughter rate of the whole ram; the slaughter rate is one of indexes of carcass meat production performance) is more than 50%, and the offspring lamb production rate of the offspring and the parent ram is more than 200%.

2. Selection of female parent:

The method comprises the steps of carrying out body type appearance, growth development and reproductive performance identification and genetic evaluation on the alexan ewe, adopting FecB gene marker auxiliary selection, IDO1 gene marker auxiliary selection, optimal linear unbiased prediction (BLUP) and genetic evaluation method of the growth performance to carry out seed selection, wherein 500 selected ewes are FecB gene++ genotype, carry IDO1 gene AA genotype, have no genetic defect, are healthy, have lambing rate of more than 105%, have adult body weight of more than 60kg, have lambing survival rate of more than 98%, and are ranked in front by using BLUP breeding value calculated by the optimal linear unbiased estimation method to form the alexan female parent basic group.

Body shape and body weight selection criteria for the alentai ewes: the meat and fat dual purpose has obvious body type, big build and equal length in the neck. The head is brown yellow, the ear is greatly drooped, the nose bridge is slightly raised, no angle is formed, and the body hair is brown red. The cross part of the nail is flat and wide, the fat buttocks are wide and thick, flat and straight or slightly sagged, the center of the lower edge is provided with a shallow longitudinal groove, the appearance is square and round, the legs are high and firm, and the hoofs are firm. The adult body weight is greater than 60kg, the height is more than 70cm, the body length is more than 72cm, the chest circumference is more than 90cm, and the tube circumference is more than 8 cm.

3. Establishing a breeding core group:

Basic core groups are established through initial selection of sheep, production performance measurement, body type appearance assessment, optimal linear unbiased prediction (BLUP), fecB gene marker assisted selection and IDO1 gene marker assisted selection (step 1 and step 2), and under normal selection intensity, the breeding core groups of each generation are determined, and seed selection and matching are carried out.

4. First generation hybridization combinations and hybridization modes:

Selecting 10 male parent multiple-embryo SAFUKE ram, performing binary hybridization with 500 standard Arletai ewes, and performing artificial insemination method for breeding at sex ratio of 1:50 to obtain 525F 1 multiple-Arrower. According to the selection and retention standard of the F1 multiple-atto sheep, 250F 1 multiple-atto sheep with standard quality are selected for seed retention.

F1 multiple atto selection criteria: fecB gene is B+ genotype, IDO1 gene is AA genotype, and BLUP breeding value (EBV) calculated by applying the optimal linear unbiased estimation method is ranked at the top. Half of the ewes have angles, and the body trunk is white or variegated; the hair of the head and limbs is brown and black, and a few are variegated. The health is firm, the nose bridge is raised, the ear is large, the neck is long, deep, wide and thick, the chest is wide and deep, the limbs are strong, and the hoof is firm. The front body is plump, and the back body has developed muscles. The tail is wider, the tail fat deposition is more, the average tail length of adult sheep is less than 13cm, the average tail circumference is less than 50cm, short hairs are externally attached, the inner surface is smooth and has no hairs, and the adult sheep is square and round. The average lambing rate is more than 140%, the weaning weight is more than 28kg, and the perillaseed weight is more than 55kg.

5. Progressive hybridization for second generation:

Continuously selecting 10 male parent multiple-embryo safock rams which reach the standard, hybridizing with 250F 1 multiple-Aja rams which reach the standard, wherein the sex ratio is 1:25, and the breeding adopts an artificial insemination method to obtain 350F 2 multiple-Aja rams. According to the F2 multiple-attomonas selection and retention standard, selecting 170F 2 multiple-attomonas selection and retention seeds reaching standards.

F2 multiple atto selection criteria: fecB gene is BB and B+ genotype, IDO1 gene is AA genotype, and BLUP breeding value (EBV) calculated by applying the optimal linear unbiased estimation method is ranked at the top. A small number of ewes have angles, the body trunk is white, and a small number of ewes are variegated; the hair of the head and the limbs is blackish brown. The body constitution is firm, the structure is symmetrical, the nose bridge is raised, the ear is large, the neck is long, deep and wide, the chest is wide and deep, the rib is well opened, the limbs are strong, the hoof is firm, and the body trunk is in a long barrel shape. The front body is plump, and the back body has developed muscles. The tail is in a chamfer shape, the upper part is wide, the lower part is narrow, the length of the tail of an adult sheep is less than 8cm, the circumference of the tail is less than 21cm, and short wool is externally attached. The lambing rate is more than 170%, the weaning body weight of the lambs is more than 28kg, and the body weight of the lambs is more than 55kg.

6. Progressive hybridization for three generations:

Continuously selecting 10 male parent multiple-embryo safock rams which reach the standard, hybridizing with 170F 2 multiple-Ayu rams which reach the standard, wherein the sex ratio is 1:17, and the breeding adopts an artificial insemination method to obtain 280F 3 multiple-Ayu rams, namely the zero generation of new variety breeding of the multiple-embryo mutton sheep.

7. And (3) transverse fixation:

Cross-fixed F3 doa standard: the ram selected FecB gene is BB genotype, the IDO1 gene is AA genotype, the ram selected FecB gene is BB and B+ genotype, the IDO1 gene is AA genotype, and BLUP breeding values (EBV) calculated by applying the optimal linear unbiased estimation method are ranked at the top. The male sheep and the female sheep have no corners, white body, and black brown head and limbs or brown. The body constitution is firm, the structure is symmetrical, the nose bridge is raised, the ear is big, the neck is long, deep and wide, the fat nail is wide and flat, and the chest is wide and deep. The back and waist are long, wide and straight, the abdomen is large and compact, the ribs are well opened, the limbs are strong, the body trunk is in a long barrel shape, and the tail is slender. The average birth weight of the male lambs is more than 3.2kg, and the average birth weight of the female lambs is more than 3kg; the average weaning weight of the male lambs is more than 30kg, and the average weaning weight of the female lambs is more than 28 kg; the average body weight of the aged ram is greater than 75kg, and the average body weight of the aged ram is greater than 55kg. The adult ram body weight is greater than 110kg, the adult ram body weight is greater than 60kg, the lambing rate is 185-220%, and the slaughtering rate is 48.5-52%.

F3 multiple sheep breeds from the flock, carries out cross fixation for more than 4 generations, comprehensively utilizes the body type appearance phenotype selection, fecB gene marker auxiliary selection, IDO1 gene marker auxiliary selection, the optimal linear unbiased estimation method (BLUP) and the genetic evaluation method of growth performance to continuously develop the breeding improvement of new strains, and continuously improves the body weight and the meat body type of the flock. The average lambing rate of the new strain of the multi-embryo mutton sheep is 201.5 percent, the average primary weights of the male lambs and the female lambs are 3.28kg and 3.11kg respectively, the average weights of the male lambs and the female lambs of 4 months of age are 36.52kg and 35.37kg respectively, the average weights of the male lambs and the female lambs of 12 months of age are 76.25kg and 56.58kg respectively, and the average weights of the adult ram and the female ram are 111.52kg and 60.16kg respectively. The rapid propagation of the core group is realized by combining Artificial Insemination (AI), synchronous estrus, superovulation and embryo transplantation (MOET) technologies, the proportion of excellent genes in the group is rapidly enlarged, and the breeding efficiency is improved. Obtaining a new strain of Xinjiang multi-embryo meat sheep, wherein the population scale of the core group of the strain reaches more than 2000.

Comparison with female parent aletai sheep: the average daily gain of the new strain of 0-90-day-old lambs is 256.97g, which is 89.19g higher than the average daily gain of the aletai sheep (167.78 g); the carcass weight of the 4-month-old male lamb is 18.57kg, which is increased by 0.85kg compared with that of the Altai male lamb (17.72 kg); the slaughtering rate of the male lambs at the age of 4 months is 50.85 percent, which is improved by 2.75 percent compared with that of the lambs at the age of alenta (48.1 percent); the eye muscle area is 17.90cm ², which is improved by 3.60cm ² compared with the eye muscle area (14.30 cm ²) of the Altai sheep (the eye muscle area refers to the area of the cross section of the back longest muscle of the livestock), and the eye muscle area is in strong correlation with the meat production performance of the livestock, so that the eye muscle area is particularly important in breeding. The weights of the ram and the ewe of 12 months old are 76.25kg and 56.58kg, respectively, which are 10.65kg and 5.98kg higher than those of the alentai ram (65.6 kg) and the ewe (50.6 kg). The tail fat weight is 0.22kg, which is reduced by 3.93kg (generally including tail fat (i.e. fat deposited at the tail) of Xinjiang local sheep such as the Oletaea sheep) compared with the tail fat weight (4.15 kg), and the tail fat weight is 8-10 kg. In conclusion, all properties are better than that of the female parent.

Comparing with male parent multiple-embryo safuck sheep: the average daily gain (250.42 g) of the sheep is improved by 6.55g compared with that of multiple-embryo safock sheep; the slaughtering rate of the male lambs of 4 months old is 50.85 percent, which is improved by 2.45 percent compared with the slaughtering rate of multiple samfick sheep (48.4 percent). The weight of the ram aged 12 months is 76.25kg, which is 6.25kg higher than that of the multiple-embryo safock sheep (70 kg). In conclusion, the average daily gain, the slaughtering rate of male lambs of 4 months old and the weight of male sheep of 12 months old are superior to those of male parents, and the other performance indexes are equivalent to those of male parents.

Body weight of the new strain was compared to high-yielding, well-known Hu sheep: the average daily gain of the new strain of 0-90-day-old lambs is 256.97g, which is improved by 56.53g compared with the average daily gain of Hu sheep (200.44 g); the carcass weight of the 4-month-old male lamb is 18.57kg, which is 0.57kg higher than that of the Hu sheep (18 kg); the average birth weight of the male lamb and the female lamb is 3.28kg and 3.11kg respectively, which are respectively increased by 0.18kg and 0.26kg compared with the average birth weight of the male lamb (3.1 kg) and the average birth weight of the female lamb (2.85 kg) of Hu sheep; average weights of male and female lambs at 4 months of age are 36.52kg and 35.37kg respectively, which are 20.74kg and 20.75kg higher than average weights of male and female lambs (15.78 kg) and (14.62 kg) of Hu sheep respectively; the average weights of the ram and the ewe of 12 months old are 76.25kg and 56.58kg respectively, which are 21.25kg and 16.58kg respectively higher than those of the ram (55 kg) and the ewe (40 kg) of Hu sheep; the average weights of adult rams and ewes were 111.52kg and 60.16kg, respectively, which were increased by 46.52kg and 15.16kg, respectively, over the average weights of Hu sheep rams (65 kg) and ewes (45 kg). In conclusion, the average daily gain, the carcass weight of the male lamb of 4 months of age, the birth weight of the lamb, the body weight of the male lamb of 4 months of age, the body weight of the male lamb of 12 months of age and the body weight of the adult lamb are all superior to those of the Hu sheep, and the breeding rate is similar to that of the Hu sheep and is higher than 200%.

The new strain lambs can be better adapted to the climate conditions of south and north Xinjiang, and the survival rate of weaned lambs is 98% and the death rate of lambs is 2% under the condition of raising and breeding in a house. The survival rate of the weaned lamb of the Arletai sheep of the Xinjiang local variety is 99.1 percent, and the death rate of the lamb is 0.9 percent. Because the new strain has high reproduction rate, the death rate of the lamb is higher than that of the aletai sheep, but the difference is not obvious and is in the normal range of 2-5% of the death rate of the lamb. Compared with the Hu sheep which is known as high-yield, the survival rate of the weaned lambs of the Hu sheep is 85 percent, the death rate of the lambs is 15 percent, and the survival rate and the death rate of the lambs of the new strain are superior to those of the Hu sheep.

In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for cultivating multi-embryo mutton sheep suitable for agriculture and animal husbandry by using Xinjiang local ewes is characterized by comprising the following steps:

2. The method of claim 1, wherein in the steps (1), (2) and (3), the male sheep which has no genetic defect, is healthy, has an average daily gain in the breeding period of more than 250g, has an adult body weight of more than 120kg, has a slaughter rate of more than 50% and has a offspring-female sheep lambing rate of more than 200% is selected as the male parent.

3. The method of claim 1, wherein in step (1), a female sheep having no genetic defect, health, a lambing rate of greater than 105%, an adult body weight of greater than 60kg, and a lambing survival rate of greater than 98% is selected as the female parent.

4. The method of claim 1, wherein in step (2), a female sheep having no genetic defect, health, a lambing rate of greater than 140%, a weaning body weight of greater than 28kg, a perigeic body weight of greater than 55kg, a tail length of less than 13cm, and a tail circumference of less than 50cm is selected as the female parent.

5. The method of claim 1, wherein in step (3), a female sheep having no genetic defect, health, a lambing rate of greater than 170%, a weaning body weight of greater than 28kg, a perigeic body weight of greater than 55kg, a tail length of less than 8cm, and a tail circumference of less than 21cm is selected as the female parent.

6. The method of claim 1, wherein in step (4), a ram with no genetic defect, health, no horn, birth weight of greater than 3.2kg, weaning weight of greater than 30kg, age of greater than 75kg, adult weight of greater than 110kg, slaughter rate of greater than 50%, and offspring lambing rate of greater than 200% of the offspring is selected as the male parent.

7. The method of claim 1, wherein in step (4), the female sheep having no genetic defect, health, no angle, birth weight of more than 3kg, weaning weight of more than 28kg, age of more than 55kg, adult weight of more than 60kg, lambing rate of more than 185%, and slaughter rate of more than 48.5% is selected as female parent.

8. The method of claim 1, wherein in step (4), the average lamb production rate of the new strain of multi-carcass ewes is greater than 200%, the average primary weights of the lambs and the ewes are 3.28kg and 3.11kg, the average weights of the 4-month-old lambs and the ewes are 36.52kg and 35.37kg, respectively, the average weights of the 12-month-old rams and the ewes are 76.25kg and 56.58kg, respectively, and the average weights of the adult rams and the ewes are 111.52kg and 60.16kg, respectively.